CN212655789U

CN212655789U - Novel intelligence bacterial examination autosegregation device

Info

Publication number: CN212655789U
Application number: CN202021923437.XU
Authority: CN
Inventors: 刘清香; 潘兴民; 张宝
Original assignee: Beijing Ainong Zhilian Technology Co ltd
Current assignee: Beijing Ainong Zhilian Technology Co ltd
Priority date: 2020-09-06
Filing date: 2020-09-06
Publication date: 2021-03-05
Anticipated expiration: 2030-09-06

Abstract

The utility model discloses a novel intelligent automatic bacteria inspection separation device, which belongs to the technical field of bacteria inspection separation, and comprises an un-inoculated culture dish storage box, a bacteria separation and inoculation working box and an inoculated culture dish storage box, wherein a cross mechanical arm and a mechanical arm are arranged on an operation platform in the bacteria separation and inoculation working box; the culture dish is automatically transported in a programmed mode, the cover of the culture dish is opened and closed, the inoculating ring is replaced, the sample is loaded through detection, the bacteria is automatically separated and scribed, the bacteria is separated and scribed on the culture medium, and the bacteria is separated and scribed in a closed space under the condition that no manual work is involved and the machine is controlled by a computer. The workload of personnel can be reduced, the bacteria separation process is more precise, the pollution to samples and environment is reduced, and the infection risk of personnel is prevented.

Description

Novel intelligence bacterial examination autosegregation device

Technical Field

The invention relates to the technical field of bacteria inspection and separation, in particular to a novel intelligent automatic bacteria inspection and separation device.

Background

In the work of bacterial detection, different bacteria in a detected solution need to be separated so as to facilitate detection of the different bacteria, and a detection sample needs to be diluted and coated on a solid culture medium; so that a single colony is isolated. When the same solution is subjected to multiple times of fractionation, the workload in the separation process is high, and the separation process is manually operated at present, so that the automation degree of equipment is low. The working efficiency is low. And some pathogenic bacteria are harmful to human bodies, if the operation is not standard, operators have infection risks, and mechanical equipment is urgently needed to replace manual operation.

Disclosure of Invention

The invention aims to provide a novel intelligent automatic bacteria inspection separating device which has the advantages that a culture medium cover is opened and covered, a sample is taken, a used dip rod is discarded, a writing robot trocar is used for marking in a culture medium to inoculate bacteria and separate the bacteria, the trocar is transported to a sterilizer to be sterilized, a bacteria storage tube is in a sealed state through arrangement, pollution is avoided, and a plurality of instruments are matched with each other to use the automatic inspection separating device, so that the problems in the background technology are solved.

In order to achieve the purpose, the invention provides the following technical scheme: a novel intelligent automatic separation device for bacterial inspection comprises a non-inoculated culture dish storage box, a bacterial separation and inoculation working box and an inoculated culture dish storage box, wherein a cross mechanical arm and a mechanical arm are arranged on an operation platform in the bacterial separation and inoculation working box;

the operation platform is provided with a conveyor belt, the conveyor belt is connected with the un-inoculated culture dish storage box and the inoculated culture dish storage box, and the conveyor belt is controlled by a computer and stops at a specified position;

the operation platform of transmission band one side is gone up the installation clean transfering loop and is stored box and abandonment transfering loop and collect the box, and the sample is examined in the opposite side installation and is examined the sample and place the district, is examining the sample and places district upper portion and be furnished with image acquisition device.

The manipulator comprises a base, a turntable, a main arm, an auxiliary arm, a machine head, a inoculating loop installer and a bottle opening claw, wherein the turntable is arranged on the base and rotates around the base, the bottom end of the main arm is sleeved on a rotating shaft of the turntable, the top end of the main arm is connected with the auxiliary arm, the other end of the auxiliary arm is connected with the machine head, the main arm, the auxiliary arm and the machine head independently rotate, the inoculating loop installer and the bottle opening claw are arranged on the machine head side by side, the inoculating loop installer is connected with a negative pressure pipe wound on the manipulator, the bottle opening claw is a mechanical claw capable of being freely opened and closed, and the inoculating loop installer is positively rotated or negatively rotated around the machine head and matched with the inoculating loop installer for opening a culture medium cover, covering the culture medium cover and installing a clean inoculating loop in the test process; for streaking media in petri dishes after taking samples, discarding the used loop.

Furthermore, an inductor and a chuck are arranged in the inoculating loop installer, a horn mouth is sleeved on the edge of the inoculating loop installer, the inductor is connected with the chuck, the inoculating loop is induced to be clamped and loosened after use.

Further, the cross arm includes the slide rail, the crossbeam, riser and transfering loop erector, the slide rail sets up the both sides at the transmission band respectively, and be parallel to each other rather than, the both ends of crossbeam are inserted in the slide rail, and slide along the slide rail, the gliding riser along length direction is gone into to the card on the crossbeam, transfering loop erector installs on the riser, and slide along vertical direction, transmission band transportation culture dish to cross arm below, transfering loop marking line is used for inoculating the bacterium in the culture medium, the separation bacterium action, be used for drawing the sample, marking line on the culture medium in the culture dish, and abandon the transfering loop that uses up.

Further, the clean inoculating loop storage box is installed at the lower part of the operation platform, a spring steel sheet, a gear and an inoculating loop are installed in the clean inoculating loop storage box, one end of the spring steel sheet is abutted to the inner wall of the clean inoculating loop storage box, the other end of the spring steel sheet is connected with one end of the inoculating loops which are stacked in a pile, the gear is connected with the other end of the inoculating loop, the gear rotates to convey the clean inoculating loop to the operation platform, and the clean inoculating loop is clamped by an inoculating loop installer of the cross-shaped mechanical arm or an inoculating loop installer of the mechanical arm.

Furthermore, an induction sealing door is installed on an upper opening in the top end of the waste inoculating loop collecting box, and after the cross-shaped mechanical arm or the mechanical arm finishes inoculation, the induction sealing door is automatically opened to receive the waste inoculating loop.

Further, the conveyer belt transports the culture dish to stay in the appointed position of operation platform in the bacteria separation inoculation work box.

Furthermore, the image acquisition device acquires the image information of the sample after the cover of the culture dish of the sample to be detected is opened by the mechanical arm.

Further, examine the sample and place the district including left drum, right drum, tray and motor setting in left drum and right drum, the motor meets with the one end of tray, places the liquid on the tray and waits to examine the solid medium of sample or after the separation.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a novel intelligent automatic bacteria inspection separation device, which is characterized in that a liquid sample inspection program and a solid culture flat plate inspection program are respectively used for clamping a fixed inoculating ring through an inoculating ring installer of a mechanical arm or a cross mechanical arm, the fixed inoculating ring is transferred to a culture dish to carry out bacteria separation and lineation according to the program, the mechanical arm is covered with a culture dish cover, the mechanical arm or the cross mechanical arm is transferred to a waste inoculating ring collecting box to discard the inoculating ring, the culture dish which is detected by the mechanical arm cover is collected into the collecting box, next detection is carried out in a circulating mode, the culture dish is automatically transported in a programmed mode, the opening and closing of the culture dish cover are carried out, the inoculating ring is replaced, sample loading is carried out, bacteria separation and lineation are carried out on a culture medium, and under the condition that no manual intervention exists, a machine is controlled. The workload of personnel can be reduced, the bacteria separation process is more precise, the pollution to samples and environment is reduced, and the infection risk of personnel is prevented.

Drawings

FIG. 1 is an overall perspective view of the present invention;

FIG. 2 is a perspective view of the robot of the present invention;

FIG. 3 is a perspective view of an inoculating loop installer of the present invention;

FIG. 4 is a cross-sectional view of a clean inoculating loop storing box of the present invention;

FIG. 5 is a view showing the structure of a waste loop collecting cassette according to the present invention;

FIG. 6 is a view showing the structure of a sample placement area to be examined according to the present invention;

FIG. 7 is a top view of a sample placement area to be examined according to the present invention;

FIG. 8 is a first inoculation procedure according to the present invention;

FIG. 9 shows a second inoculation procedure of the present invention.

In the figure: 1. a non-inoculated culture dish storage box; 2. a bacteria separation and inoculation work box; 3. a culture dish storage box after inoculation; 4. an operating platform; 5. a cross-shaped mechanical arm; 51. a slide rail; 52. a cross beam; 53. a vertical plate; 6. a manipulator; 61. a base; 62. a turntable; 63. a main arm; 64. an auxiliary arm; 65. a machine head; 66. an inoculating loop installer; 661. an inductor; 662. a chuck; 663. a bell mouth; 67. a bottle opening claw; 7. a conveyor belt; 8. Clean inoculating loop storage box; 81. a spring steel sheet; 82. a gear; 9. a waste inoculating loop collecting box; 91. an induction closing door; 10. a sample placement area to be detected; 101. a left cylinder; 102. a right cylinder; 103. a tray; 104. A motor; 110. an image acquisition device.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, a novel intelligent automatic bacteria inspection separation device comprises an un-inoculated culture dish storage box 1, a bacteria separation and inoculation work box 2 and an inoculated culture dish storage box 3, wherein a cross-shaped mechanical arm 5 and a mechanical arm 6 are arranged on an operation platform 4 in the bacteria separation and inoculation work box 2;

the operating platform 4 is provided with a conveyor belt 7, the conveyor belt 7 is connected with the un-inoculated culture dish storage box 1 and the inoculated culture dish storage box 3, and the conveyor belt 7 is controlled by a computer and stops at a specified position;

the operation platform 4 of transmission band 7 one side goes up the installation clean transfering loop and stores box 8 and abandonment transfering loop and collect box 9, and the sample is examined in the opposite side installation and is examined sample and place district 10, is equipped with image acquisition device 110 on examining sample and place district 10 upper portion.

The cross mechanical arm 5 comprises slide rails 51, a cross beam 52, a vertical plate 53 and a inoculating loop installer 66, wherein the slide rails 51 are respectively arranged on two sides of the conveying belt 7 and are parallel to each other, two ends of the cross beam 52 are inserted into the slide rails 51 and slide along the slide rails 51, the vertical plate 53 sliding along the length direction is clamped on the cross beam 52, the inoculating loop installer 66 is installed on the vertical plate 53 and slides along the vertical direction, the conveying belt 7 conveys a culture dish to the lower side of the cross mechanical arm 5, and the inoculating loop is scribed in the culture medium for inoculating bacteria and separating bacteria and is used for scribing on the culture medium in the culture dish when a sample is extracted and used up, and the used inoculating loop is discarded.

The conveyor belt 7 transports the culture dish to stay at a designated position of the operation platform 4 in the bacteria separation and inoculation work box 2.

The image acquisition device 110 acquires image information of a sample after the cover of the culture dish of the sample to be detected is opened by the manipulator 6.

Referring to fig. 2, the manipulator 6 includes a base 61, a turntable 62, a main arm 63, an auxiliary arm 64, a machine head 65, a inoculating loop mounting device 66 and a bottle opening claw 67, the turntable 62 is disposed on the base 61 and rotates around the base 61, the bottom end of the main arm 63 is sleeved on the rotating shaft of the turntable 62, the top end of the main arm 63 is connected with the auxiliary arm 64, the other end of the auxiliary arm 64 is connected with the machine head 65, the main arm 63, the auxiliary arm 64 and the machine head 65 rotate independently, the inoculating loop mounting device 66 and the bottle opening claw 67 are disposed on the machine head 65 side by side, the inoculating loop mounting device 66 is connected with a negative pressure pipe wound on the manipulator 6, the bottle opening claw 67 is a mechanical claw capable of freely opening and closing, and rotates forwards or backwards around the machine head 65 and is matched with the inoculating loop mounting device 66, and is used for opening a culture medium cover, covering and mounting a clean inoculating loop in; for streaking media in petri dishes after taking samples, discarding the used loop.

Referring to fig. 3, the inoculating loop mounter 66 is provided with a built-in sensor 661 and a chuck 662, and a bell mouth 663 is sleeved on the edge of the inoculating loop mounter 66, the sensor 661 is connected with the chuck 662 to sense the clamping of the inoculating loop and then release the inoculating loop after use, when the inoculating loop touches the sensor 661, the chuck 662 contracts to clamp and fix the inoculating loop, the bell mouth 663 is used for pouring the inoculating loop into the chuck 662, and when the robot 6 or the cross robot 5 moves onto the waste inoculating loop collecting box 9, the sensed chuck 662 automatically opens to discard the waste.

Referring to fig. 4, the clean inoculating loop storing box 8 is installed at the lower part of the operation platform 4, a spring steel sheet 81, a gear 82 and an inoculating loop are installed in the clean inoculating loop storing box 8, one end of the spring steel sheet 81 is abutted against the inner wall of the clean inoculating loop storing box 8, the other end is connected with one end of the inoculating loops stacked in a pile, the gear 82 is connected with the other end of the inoculating loop, and the gear 82 rotates to convey the clean inoculating loop onto the operation platform 4 and is clamped by the inoculating loop mounting device 66 of the cross robot 5 or the inoculating loop mounting device 66 of the robot 6.

Referring to fig. 5, an induction closing door 91 is installed on an upper opening of the top end of the waste inoculating loop collecting box 9, and after the cross-shaped mechanical arm 5 or the mechanical arm 6 finishes inoculating, the induction closing door 91 is automatically opened to receive the waste inoculating loop.

Referring to fig. 6, the sample placing area 10 includes a left cylinder 101, a right cylinder 102, a tray 103 and a motor 104, the tray 103 and the motor 104 are disposed in the left cylinder 101 and the right cylinder 102, the motor 104 is connected to one end of the tray 103, a liquid sample to be tested or a separated solid culture medium is placed on the tray 103, a groove for placing a test tube is formed in the liquid sample to be tested, meanwhile, a culture dish is disposed in the right cylinder 102, the two trays 103 are alternately pushed upwards in sequence during movement, after collection is completed, the left cylinder 101 is pushed by the manipulator 6 to receive the culture dish for sampling, and the tray 103 is driven by rotation of the motor 104 to move up and down in the left cylinder 101 and the right cylinder 102 respectively.

The first embodiment is as follows:

liquid sample testing procedure:

opening a novel intelligent automatic bacteria inspection separating device and computer equipment, selecting a corresponding program, stirring and uniformly mixing samples such as tissues to be inspected, secretions, bacterial colonies, environments and the like, adding the samples into liquid sample loading equipment, pushing the novel intelligent automatic bacteria inspection separating device, transporting a clean culture dish to a fixed position in a bacteria separation and inoculation working box 2 from a non-inoculated culture dish storage box 1, opening a cover of the culture dish by a manipulator 6, rotating the clean inoculating loop storage box 8 to a gear 82 of the bacteria separation and inoculation working box 2, and pushing an inoculating loop out of an operating platform 4; the inoculating loop is clamped and fixed by an inoculating loop mounting device 66 of the manipulator 6 or the cross-shaped mechanical arm 5, the inoculating loop is moved to a culture dish to carry out bacteria separation and scribing according to the program, and the manipulator 6 covers the culture dish. The manipulator 6 or the cross-shaped mechanical arm 5 operates to the waste inoculating loop collecting box 9 to discard the inoculating loop, and the next detection is carried out in a circulating mode.

Example two:

solid culture plate detection procedure:

opening a novel intelligent automatic bacteria inspection separating device and computer equipment, selecting a corresponding program, pushing a culture dish to be detected upwards from a sample placing area 10 to be detected, opening the cover of the culture dish by a manipulator 6, collecting bacterial colony photos by an image collecting device 110, transmitting the bacterial colony photos to a computer, selecting selected bacterial colonies by an operator (or a computer program) according to the characteristics of the bacterial colony forms, colors and the like, conveying a clean culture dish by a conveying belt 7, opening the cover, and pushing a inoculating loop out of a bacteria separation and inoculation working box 2 by a clean inoculating loop storage box 8; the fixed inoculating loop is clamped by an inoculating loop installer 66 of a manipulator 6 or a cross-shaped mechanical arm 5, bacteria are separated and scribed according to the program on the culture dish in an operation mode, the manipulator 6 covers the cover of the culture dish, the manipulator 6 or the cross-shaped mechanical arm 5 operates the abandoned inoculating loop collecting box 9 to discard the inoculating loop, the culture dish which is detected by the manipulator 6 covers is collected into the collecting box, and the next detection is carried out in a circulating mode.

Through utilizing the computer to cross arm 5, manipulator 6, transmission band 7, clean transfering loop storage box 8, abandonment transfering loop collection box 9 and wait to examine equipment control operation such as sample placement area 10, the transportation of automatic programming completion culture dish, culture dish lid switch, change transfering loop, the sample that detects is gone up appearance, automatic bacteria separation is rule, the completion is drawn line the separation on the culture medium bacterium, under the condition that does not have artifical participation, by computer control machinery in the confined space, accomplish the bacteria separation and draw line. The workload of personnel can be reduced, the bacteria separation process is more precise, the pollution to samples and environment is reduced, and the infection risk of personnel is prevented.

In summary, the following steps: this novel intelligence bacterial examination autosegregation device, through liquid sample inspection procedure and solid culture flat board detection procedure, get fixed transfering loop through transfering loop erector 66 clamp of manipulator 6 or cross arm 5 respectively, move on to the culture dish and rule according to procedure bacterial separation, manipulator 6 covers the culture dish lid, manipulator 6 or cross arm 5 move abandonment transfering loop collection box 9 and abandon the transfering loop, the culture dish that manipulator 6 lid detected is collected and is hidden the box, the circulation carries out next and detects, the transportation of automatic programming completion culture dish, culture dish lid switch, change transfering loop, the sample is gone up to the testing sample, automatic bacterial separation is rule, accomplish the bacterial separation and rule on the culture medium, under the condition that does not have artifical participation, by computer control machinery in the confined space, accomplish the bacterial separation and rule. The workload of personnel can be reduced, the bacteria separation process is more precise, the pollution to samples and environment is reduced, and the infection risk of personnel is prevented.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.

Claims

1. A novel intelligent automatic bacteria inspection separation device is characterized by comprising an un-inoculated culture dish storage box (1), a bacteria separation and inoculation work box (2) and an inoculated culture dish storage box (3), wherein a cross mechanical arm (5) and a mechanical arm (6) are arranged on an operation platform (4) in the bacteria separation and inoculation work box (2);

the operation platform (4) is provided with a conveyor belt (7), the conveyor belt (7) is connected with the un-inoculated culture dish storage box (1) and the inoculated culture dish storage box (3), and the conveyor belt (7) is controlled by a computer and stops at a specified position;

the operation platform (4) of transmission band (7) one side is gone up the installation clean transfering loop and is stored box (8) and abandonment transfering loop and collect box (9), and the sample is examined in the opposite side installation and is examined the sample and place district (10), is being examined sample and is placed district (10) upper portion and be furnished with image acquisition device (110).

2. The novel intelligent automatic bacteria inspection separating device as claimed in claim 1, wherein the manipulator (6) comprises a base (61), a turntable (62), a main arm (63), an auxiliary arm (64), a machine head (65), a inoculating loop installer (66) and a bottle opening claw (67), the turntable (62) is arranged on the base (61) and rotates around the base (61), the bottom end of the main arm (63) is sleeved on a rotating shaft of the turntable (62), the top end of the main arm (63) is connected with the auxiliary arm (64), the other end of the auxiliary arm (64) is connected with the machine head (65), the main arm (63), the auxiliary arm (64) and the machine head (65) independently rotate, the inoculating loop installer (66) and the bottle opening claw (67) are arranged on the machine head (65) side by side, the inoculating loop installer (66) is connected with a negative pressure pipe wound on the manipulator (6), and the bottle opening claw (67) is a mechanical claw capable of freely opening and closing, the winding machine head (65) rotates forwards or backwards and is matched with an inoculating loop installer (66) for opening a culture medium cover, covering the culture medium cover and installing a clean inoculating loop in the test process; for streaking media in petri dishes after taking samples, discarding the used loop.

3. The novel intelligent automatic bacteria inspection separating device as claimed in claim 2, wherein the inoculating loop mounting device (66) is internally provided with a sensor (661) and a chuck (662), a bell mouth (663) is sleeved on the edge of the inoculating loop mounting device (66), the sensor (661) is connected with the chuck (662), and the inoculating loop is sensed and clamped and loosened after use.

4. The novel intelligent automatic bacteria inspection separating device as claimed in claim 1, wherein the cross mechanical arm (5) comprises a slide rail (51), a cross beam (52), a vertical plate (53) and an inoculating loop mounting device (66), the slide rail (51) is respectively arranged at two sides of the conveying belt (7), and are parallel to each other, two ends of the beam (52) are inserted into the slide rails (51) and slide along the slide rails (51), the beam (52) is clamped into a vertical plate (53) sliding along the length direction, the inoculating loop mounting device (66) is mounted on the vertical plate (53), and slides along the vertical direction, the transmission belt (7) transports the culture dish to the lower part of the cross mechanical arm (5), the inoculating ring (54) is lined in the culture medium for inoculating bacteria and separating bacteria, for streaking on media in a petri dish after taking a sample, and discarding the used loop.

5. The novel intelligent automatic bacteria inspection separating device as claimed in claim 1, wherein the clean inoculating loop storage box (8) is installed at the lower part of the operating platform (4), the spring steel sheet (81) and the gear (82) are installed in the clean inoculating loop storage box (8), one end of the spring steel sheet (81) is abutted against the inner wall of the clean inoculating loop storage box (8), the other end of the spring steel sheet is connected with one end of the inoculating loops stacked in a pile, the gear (82) is connected with the other end of the inoculating loops, the gear (82) rotates to convey the clean inoculating loops to the operating platform (4), and the clean inoculating loops are clamped by the inoculating loop installer (66) of the cross mechanical arm (5) or the inoculating loop installer (66) of the mechanical arm (6).

6. The novel intelligent automatic bacteria test separation device as claimed in claim 1, wherein an induction closing door (91) is installed on an upper opening at the top end of the waste inoculating loop collecting box (9), and after the cross-shaped mechanical arm (5) or the mechanical arm (6) finishes inoculation, the induction closing door (91) is automatically opened to receive the waste inoculating loop.

7. A novel intelligent automatic bacteria inspection separating device as claimed in claim 1, characterized in that the conveyor belt (7) transports the culture dish to stay at the designated position of the operating platform (4) in the bacteria separation and inoculation work box (2).

8. The novel intelligent automatic bacteria test separation device as claimed in claim 1, wherein the image acquisition device (110) acquires image information of the sample after the cover of the culture dish of the sample to be tested is opened by the mechanical arm (6).

9. The novel intelligent automatic bacteria test separation device as claimed in claim 1, wherein the sample placement area (10) to be tested comprises a left cylinder (101), a right cylinder (102), a tray (103) and a motor (104), the tray (103) and the motor (104) are arranged in the left cylinder (101) and the right cylinder (102), the motor (104) is connected with one end of the tray (103), and the liquid sample to be tested or the separated solid culture medium is placed on the tray (103).