CN212247053U - Edible fungus fermentation production device with near infrared spectrum detector - Google Patents

Edible fungus fermentation production device with near infrared spectrum detector Download PDF

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Publication number
CN212247053U
CN212247053U CN201922189831.9U CN201922189831U CN212247053U CN 212247053 U CN212247053 U CN 212247053U CN 201922189831 U CN201922189831 U CN 201922189831U CN 212247053 U CN212247053 U CN 212247053U
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China
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near infrared
infrared spectrum
sample
edible fungus
splitting system
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CN201922189831.9U
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马玉涵
黄青
何华奇
孙玉军
李坤
赵岩
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Anhui University of Science and Technology
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Anhui University of Science and Technology
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Abstract

The utility model discloses an edible fungus fermentation production device provided with a near infrared spectrum detector, which comprises a fermentation tank body, wherein a sampling device and a near infrared spectrum detection device are arranged on the side wall of the fermentation tank body; the sampling device comprises a sample conveying pipe and a self-sucking pump; the near infrared spectrum detection device comprises an upper computer and a spectrometer, the spectrometer comprises a first shell, a light source system, a sample analysis system and a light splitting system are fixed in the first shell through a support, a slit is formed in the left side wall of the light splitting system, close to the sample analysis system, and a power supply and a single chip microcomputer are mounted on the upper portion of the left inner wall of the first shell; the optical splitting system is internally and fixedly provided with a grating at the upper left corner through a bracket, the lower part of the optical splitting system is provided with a first spherical reflector, the right side of the optical splitting system is provided with a second spherical reflector, and the upper part of the optical splitting system is provided with a signal acquisition module and a CCD sensor. The utility model discloses need not carry out the preliminary treatment to the sample, solved the difficult problem of quality witnessed inspections analysis in the domestic fungus fermentation production process.

Description

Edible fungus fermentation production device with near infrared spectrum detector
Technical Field
The utility model relates to a fermentation equipment technical field specifically is an edible mushroom fermentation apparatus for producing with near infrared spectrum detector.
Background
The edible fungi in China are abundant in resources and are one of the earliest countries for cultivating and utilizing the edible fungi. The edible fungus extract contains rich protein and amino acid, and the content of the edible fungus extract is several times to dozens of times of that of common vegetables and fruits; the polysaccharide is characterized by biological response modifier, and can be used as immunopotentiator and immune activator, and the active polysaccharide has a structure, i.e. its main chain is formed from beta-D (1-3) connected glucosyl, and the glucosyl connected with beta-D (1-3) is randomly distributed along the main chain and formed into comb-like structure, and the size of organism can be changed according to the fine structure and conformation of polysaccharide.
According to the research, the active polysaccharide component beta-D (1-3) glucose of the medicinal bacteria has changes to heterogenous, homogenous and even hereditary tumors. In addition, it also has antibacterial, antiviral and anti-coagulation effects, and has effects of improving liver function and detoxication, improving animal anoxia resistance and oxygen utilization rate, reducing blood viscosity, increasing myocardial contractility, improving heart rhythm, lowering blood sugar, tranquilizing, relieving pain, relieving asthma, relieving cough, and eliminating phlegm.
The content of the polysaccharide in the edible fungi is directly related to the quality of a final product, so that the content of the polysaccharide needs to be detected in the production process. Currently, many different methods are used for polysaccharide detection, such as: the chemical method comprises the following steps: phenol sulfuric acid process, anthrone sulfuric acid process and 3, 5-dinitrosalicylic acid (DNS) process. Chromatography: gas chromatography, liquid chromatography, high performance anion exchange chromatography, and volume exclusion chromatography and thin layer chromatography. Capillary electrophoresis. These common conventional analytical methods are time consuming, labor intensive, material consuming and complex to operate and cause damage and contamination to the sample.
The near infrared spectroscopy is a novel spectral analysis method which is developed rapidly in the field of analytical chemistry in recent years, and has the characteristics of rapidness, simultaneous measurement of various physicochemical properties, environmental friendliness, convenience in operation and the like. The near infrared spectrum is the electromagnetic spectrum between the visible and mid-infrared, with wave numbers of about: 12500-4000 cm-1. The near infrared spectrum method is to utilize the stretching vibration frequency doubling and frequency combining of chemical bonds (X-H) containing hydrogen groups (X-H, X is C, O, N, S and the like), to correlate the near infrared absorption spectrum of a corrected sample with the component concentration or property data by selecting a proper chemometrics multivariate correction method in the absorption spectrum of a near infrared region, and to establish a relation-correction model between the absorption spectrum of the corrected sample and the component concentration or property of the corrected sample. When the unknown sample is predicted, the component concentration or the property of the unknown sample can be quantitatively predicted by applying the established correction model and the absorption spectrum of the unknown sample. In addition, by selecting a proper chemometrics pattern recognition method, the near infrared absorption spectrum characteristic information of the sample can be separated and extracted, and a corresponding model is established. When the classification of the unknown sample is carried out, the attribution of the unknown sample can be qualitatively judged by applying the established model and the absorption spectrum of the unknown sample.
If the infrared spectrum detection can be combined with the traditional edible fungus fermentation production device, great convenience can be brought to production.
SUMMERY OF THE UTILITY MODEL
In order to solve the existing problems, the utility model provides an edible fungus fermentation production device with a near infrared spectrum detector. The utility model discloses a following technical scheme realizes.
An edible fungus fermentation production device provided with a near infrared spectrum detector comprises a fermentation tank body, wherein a sampling device and a near infrared spectrum detection device are arranged on the side wall of the fermentation tank body;
the sampling device comprises a sample conveying pipe and a self-sucking pump; the near infrared spectrum detection device comprises an upper computer and a spectrometer, the spectrometer comprises a first shell, a light source system, a sample analysis system and a light splitting system are fixed in the first shell through a support, a slit is formed in the left side wall of the light splitting system, close to the sample analysis system, and a power supply and a single chip microcomputer are mounted on the upper portion of the left inner wall of the first shell;
the self-priming pump is connected with the sample analysis system through a liquid conveying pipe.
Preferably, a grating is fixedly arranged in the upper left corner of the light splitting system through a support, a first spherical reflector is arranged at the lower part of the light splitting system, a second spherical reflector is arranged at the right side of the light splitting system, and a signal acquisition module and a CCD sensor are arranged at the upper part of the light splitting system.
Preferably, the light source system is composed of a tungsten halogen lamp and a lens.
Preferably, the sample analysis system is provided with a stepper motor at the lower part of the rotary sample cell.
Preferably, the halogen tungsten lamp, the lens, the slit and the center point of the second spherical reflector are on the same straight line.
Preferably, the optical axes of the first spherical reflector and the second spherical reflector are symmetrical to the central normal of the grating.
Preferably, sample conveyer pipe and self priming pump outside are equipped with the second casing, the second casing passes through the fastener rigid coupling and is in the inside wall of fermentation cylinder body.
More preferably, the first casing side wall is fixedly connected to the outer side wall of the fermenter body by a fastener.
The utility model has the advantages that:
the utility model relates to an edible mushroom fermentation apparatus for producing with near infrared spectrum detector includes the fermentation cylinder body, fermentation cylinder body inside wall rigid coupling has sampling device, the rigid coupling has near infrared spectrum detection device on the lateral wall, the automatic sample of accomplishing of entire system, detect, can realize the spectral signal of its diffuse transmission of same sample at different angular measurements, can eliminate the measuring error that the sample loading is inhomogeneous to arouse, can be accurate carry out quantitative determination to compositions such as polysaccharide in the edible mushroom fermentation production, the degree of accuracy of the speed and the data that have improved the near infrared spectrum of sample and detect, need not carry out the preliminary treatment to the sample, the difficult problem of quality on-the-spot detection analysis in the edible mushroom fermentation production process has been solved.
Drawings
FIG. 1 is a schematic structural diagram of an edible fungus fermentation production device provided with a near infrared spectrum detector;
FIG. 2 is a partial structure diagram of the edible fungus fermentation production device provided with the near infrared spectrum detector of the utility model;
fig. 3 is the schematic block diagram of the signal acquisition system of the present invention.
Wherein: 1. a fermenter body; 2. a sampling device; 201. a sample delivery tube; 202. a self-priming pump; 203. a second housing; 3. a near infrared spectrum detection device; 4. an upper computer; 5. a spectrometer; 6. a first housing; 7. a light source system; 701. a halogen tungsten lamp; 702. a lens; 8. a sample analysis system; 801. a sample rotating pool; 802. a stepping motor; 9. a light splitting system; 10. a power source; 11. a single chip microcomputer; 12. a grating; 13. a first spherical mirror; 14. a second spherical mirror; 15. a signal acquisition module; a CCD sensor; 17. a timing control module; 18. a signal amplification module; 19. a digital-to-analog conversion module; 20 a data buffer module; 21. a slit.
Detailed Description
The technical solution of the present invention will be described in more detail and fully with reference to the accompanying drawings.
An edible fungus fermentation production device provided with a near infrared spectrum detector comprises a fermentation tank body 1, wherein a sampling device 2 and a near infrared spectrum detection device 3 are arranged on the side wall of the fermentation tank body 1; the sampling device 2 comprises a sample conveying pipe 201 and a self-priming pump 202; the near infrared spectrum detection device 3 comprises an upper computer 4 and a spectrometer 5, the spectrometer 5 comprises a first shell 6, a light source system 7, a sample analysis system 8 and a light splitting system 9 are fixed in the first shell 6 through a support, a slit 21 is formed in the left side wall of the light splitting system 9 close to the sample analysis system 8, and a power supply 10 and a single chip microcomputer 11 are mounted on the upper portion of the left inner wall of the first shell 6; the self-priming pump 202 is connected to the sample analysis system 8 by a fluid transfer line.
Further optimally, a grating 12 is fixedly arranged in the light splitting system 9 at the upper left corner through a bracket, a first spherical reflector 13 is arranged at the lower part, a second spherical reflector 14 is arranged at the right side, and a signal acquisition module 15 and a CCD sensor 16 are arranged at the upper part; the light source system 7 is composed of a halogen tungsten lamp 701 and a lens 702; the sample analysis system 8 is equipped with a stepping motor 802 at the lower part of the rotary sample cell 801.
In a further optimized scheme, the halogen tungsten lamp 701, the lens 702, the slit 21 and the central point of the second spherical reflector 14 are on the same straight line; the optical axes of the first spherical reflector 13 and the second spherical reflector 14 are symmetrical to the central normal of the grating 12.
And finally, further optimization is carried out, a second shell 203 is arranged outside the sample conveying pipe 201 and the self-sucking pump 202, the second shell 203 is fixedly connected to the inner side wall of the fermentation tank body 1 through a fastener, and the side wall of the first shell 6 is fixedly connected to the outer side wall of the fermentation tank body through a fastener.
Detailed description of the preferred embodiment 1
The utility model provides an edible mushroom fermentation apparatus for producing with near infrared spectrum detector, includes conventional edible mushroom fermentation production's fermentation cylinder body 1, has through the fastener rigid coupling on the lateral wall of fermentation cylinder body 1 to be equipped with sampling device 2 and near infrared spectrum detection device 3. The sampling device 2 comprises a sample conveying pipe 201 and a self-priming pump 202; sample conveyer pipe 201 and self priming pump 202 outside are equipped with second casing 203, and second casing 203 is in through the fastener rigid coupling the inside wall of fermentation cylinder body 1. The sample conveying pipe 201 is a telescopic pipeline and can be adjusted according to the height of the liquid level in the fermentation tank; the second housing 203, which is external to the sample delivery tube, protects the self-primer pump 202 and the sample delivery tube from external fermentation broth.
The near infrared spectrum detection device 3 comprises an upper computer 4 and a spectrometer 5, the spectrometer 5 comprises a first shell 6, and the side wall of the first shell 6 is fixedly connected to the outer side wall of the fermentation tank body through a fastener; a light source system 7, a sample analysis system 8 and a light splitting system 9 are fixed in the first shell 6 through a support, a slit 21 is formed in the left side wall of the light splitting system 9 close to the sample analysis system 8, and a power supply 10 and a single chip microcomputer 11 are mounted on the upper portion of the left inner wall of the first shell 6; the self-priming pump 202 is connected to the sample analysis system 8 by a fluid transfer line.
A grating 12 is fixedly arranged in the light splitting system 9 at the upper left corner through a bracket, a first spherical reflector 13 is arranged at the lower part, a second spherical reflector 14 is arranged at the right side, and a signal acquisition module 15 and a CCD sensor 16 are arranged at the upper part; the light source system 7 is composed of a tungsten halogen lamp 701 and a lens 702. The halogen tungsten lamp 701, the lens 702, the slit 21 and the center point of the second spherical reflector 14 are on the same straight line. The optical axes of the first spherical reflector 13 and the second spherical reflector 14 are symmetrical to the central normal of the grating 12, so as to form a diffuse transmission measuring device for the solid sample.
Further, the sample analysis system 8 is provided with a stepping motor 802 at the lower part of the rotary sample cell 801.
The fermentation sample of the edible fungi is placed in the sample rotating pool 801, the composite light emitted by the light source system 7 is diffused and transmitted by the sample and then enters the light splitting system 9, the single chip microcomputer 11 controls the stepping motor 802 to rotate according to the sampling times N set by a user to drive the sample rotating pool 801 to rotate by corresponding angles of 360 degrees/N in sequence, and meanwhile, the signal acquisition module 15 samples and uploads the samples to the upper computer 4. And taking the average value of the N times of data as a detection signal of the sample, thereby eliminating the measurement error caused by uneven loading of the components of the edible fungus fermentation liquid sample. And the upper computer 4 obtains the absorbance spectrum data of the sample according to the detection signals of the sample, the blank and the background, displays the spectrogram, and analyzes the components of the edible fungus fermentation liquid sample through an analysis module.
The blank detection signal is obtained by emptying the sample rotating tank 801, passing the composite light emitted by the light source system 7 through the empty sample rotating tank 801 and then entering the light splitting system 9, and sampling the obtained signal by the signal acquisition module 15 to obtain the blank detection signal.
The background detection signal is obtained by covering the entrance slit 21 of the light splitting system 9, so that the external light does not enter the light splitting system 9, and the signal obtained by sampling by the signal acquisition module 15 is the background detection signal.
The single chip microcomputer 11 adopts a single chip microcomputer integrated with a USB2.0 controller, is fixed on a bottom plate of a first shell 16 of the instrument, controls modules such as a time sequence control module 17, a signal amplification processing module 18, a digital-to-analog conversion module 20, a data cache 21 and the like and control signals generated by a stepping motor 802, is connected with the modules through data or control lines, and is in instruction and data communication with the upper computer 4 through a USB interface.
The power supply 8 is fixed on the left side wall of the first shell 16 of the instrument, the power supply 8 can be alternating current or a storage battery, a voltage-stabilized power supply is converted into direct current 24V through a DA-DA module, power lines are connected with all systems to provide required voltage for the light source system 7, the signal acquisition module 15 and the single chip microcomputer 11, the power supply is connected with an external power supply through a power socket 22 and a series switch 23, and the on-off of the power supply of the instrument is controlled by a switch button.
The method comprises the steps that sample components of edible fungus fermentation liquid in a sample rotating pool 801 are transmitted by composite light diffuse emitted by a light source system 7 and then are emitted into a light splitting system 9 through a slit 21 to be split to obtain near-infrared monochromatic light with a specified waveband, a series of monochromatic spectral lines with the wavelength of 700-plus-1100 nm are formed on the receiving surface of a CCD sensor 16, photoelectric conversion is carried out on the spectral lines by a signal acquisition module 15, and then the spectral lines are sent to a single chip microcomputer 7, wherein the signal acquisition module 15 is composed of a time sequence control module 17, the CCD sensor 15, a signal amplification processing module 19, a digital-to-analog conversion module 19 and a data. The timing control block 17 generates a CCD driving signal, an a/D conversion signal, and a writing signal of the data storage block 21. The electric signal generated by the CCD sensor 16 is driven by the timing control module 17 and then output, amplified by the signal amplification processing module 18, and then enters the data buffer module 20 through the analog-to-digital conversion module 19 for buffering, and then is transmitted to the upper computer 4 through the USB port.
The single chip microcomputer 11 controls the stepping motor 802 to drive the sample rotating tank 801 to rotate, so that the diffuse transmission spectrum signals of the same sample are measured at different angles, the average value of the diffuse transmission spectrum signals is taken as the detection signal of the sample, and the measurement error caused by uneven loading of the components of the edible fungus fermentation liquid sample is eliminated. And the upper computer 4 obtains the absorbance spectrum data of the sample according to the detection signals of the sample, the blank and the background, displays the spectrum diagram of the absorbance spectrum data and analyzes the components of the edible fungus fermentation liquid sample.
It is to be understood that the described embodiments are merely individual embodiments of the invention, rather than all embodiments. Based on the embodiments in the present invention, all other implementations obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.

Claims (8)

1. The utility model provides an edible mushroom fermentation apparatus for producing with near infrared spectrum detector, includes fermentation cylinder body (1), its characterized in that:
a sampling device (2) and a near infrared spectrum detection device (3) are arranged on the side wall of the fermentation tank body (1);
the sampling device (2) comprises a sample conveying pipe (201) and a self-sucking pump (202);
the near infrared spectrum detection device (3) comprises an upper computer (4) and a spectrometer (5), the spectrometer (5) comprises a first shell (6), a light source system (7), a sample analysis system (8) and a light splitting system (9) are fixed in the first shell (6) through a support, a slit (21) is formed in the left side wall of the light splitting system (9) close to the sample analysis system (8), and a power supply (10) and a single chip microcomputer (11) are mounted on the upper portion of the left inner wall of the first shell (6);
the self-priming pump (202) is connected with the sample analysis system (8) through a liquid conveying pipe.
2. The edible fungus fermentation production device provided with the near infrared spectrum detector according to claim 1, wherein: a grating (12) is fixedly arranged in the upper left corner of the light splitting system (9) through a bracket, a first spherical reflector (13) is arranged at the lower part of the light splitting system, a second spherical reflector (14) is arranged at the right side of the light splitting system, and a signal acquisition module (15) and a CCD sensor (16) are arranged at the upper part of the light splitting system.
3. The edible fungus fermentation production device provided with the near infrared spectrum detector according to claim 2, characterized in that: the light source system (7) is composed of a halogen tungsten lamp (701) and a lens (702).
4. The edible fungus fermentation production device provided with the near infrared spectrum detector according to claim 1, wherein: the sample analysis system (8) is provided with a stepping motor (802) at the lower part of a rotary sample cell (801).
5. The edible fungus fermentation production device provided with the near infrared spectrum detector according to claim 3, wherein the edible fungus fermentation production device comprises: the halogen tungsten lamp (701), the lens (702), the slit (21) and the center point of the second spherical reflector (14) are on the same straight line.
6. The edible fungus fermentation production device provided with the near infrared spectrum detector according to claim 2, characterized in that: and the optical axes of the first spherical reflector (13) and the second spherical reflector (14) are symmetrical to the central normal of the grating (12).
7. The edible fungus fermentation production device provided with the near infrared spectrum detector according to claim 1, wherein: sample conveyer pipe (201) and self priming pump (202) outside are equipped with second casing (203), second casing (203) are in through the fastener rigid coupling the inside wall of fermentation cylinder body (1).
8. The edible fungus fermentation production device provided with the near infrared spectrum detector according to claim 1, wherein: the side wall of the first shell (6) is fixedly connected to the outer side wall of the fermentation tank body through a fastener.
CN201922189831.9U 2019-12-10 2019-12-10 Edible fungus fermentation production device with near infrared spectrum detector Expired - Fee Related CN212247053U (en)

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