CN108982627A - A kind of detection method of enzyme-free glucose optical electro-chemistry sensor and concentration of glucose - Google Patents
A kind of detection method of enzyme-free glucose optical electro-chemistry sensor and concentration of glucose Download PDFInfo
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Abstract
The present invention relates to electrochemical analysis testing fields, in particular to the detection method of a kind of enzyme-free glucose optical electro-chemistry sensor and concentration of glucose.A kind of enzyme-free glucose optical electro-chemistry sensor, with TiO2Nano-stick array thin film electrode is working electrode, and platinum filament is used as to electrode, and saturated calomel electrode is as reference electrode.Enzyme-free glucose optical electro-chemistry sensor provided by the invention, which is that one kind is environmental-friendly, prepares simple, low in cost, high sensitivity one kind, is based on TiO2The enzyme-free glucose optical electro-chemistry sensor of nano-stick array thin film electrode, based on the photoelectric current detection under light excitation, with mutually independent excitaton source and electrochemical signals detection system, detection signal background is low, signal is strong, without expensive instrument and equipment, just have higher detection sensitivity, and selectively good and stability is good, provides a simplicity, cheap new way for glucose detection.
Description
Technical field
The present invention relates to electrochemical analysis testing fields, sense in particular to a kind of enzyme-free glucose optical electro-chemistry
The detection method of device and concentration of glucose.
Background technique
The quantitative analysis of glucose has extremely heavy in fields such as clinical medicine, biochemistry, environmental monitoring, Food Sciences
The effect wanted, exploitation glucose sensor are the emphasis of sensor field development all the time.Currently, the measuring method of glucose
Mainly there are infra-red sepectrometry, capillary electrophoresis, fluorescent spectrometry, photocaustic spectroscopy, chromatmetry, surface plasma body resonant vibration raw
Object sensor and electrogenerated chemiluminescence method.But these methods usually require more expensive instrument and equipment and more complex sample
Processing, is unfavorable for promoting.
In contrast, electrochemica biological sensor by its higher sensitivity, expense is lower, easy to operate, sample disappears
The advantages that small is consumed, there is biggish application potential.However, the glucose oxidase electrochemical sensor that limitation is studied extensively, by
It is limited in the physicochemical properties by enzyme, makes glucose oxidase be not easy to fix on the electrode for a long time, while being easy by temperature
The interference of the factors such as degree and chemical environment, has an impact the testing result of glucose.In addition, in recent years, enzyme-free glucose passes
Though sensor causes extensive research, sensors with auxiliary electrode is mostly based on the nanoparticles such as gold, platinum, copper and its precious metal alloys,
Have at high cost, poor selectivity and it is unstable the defects of, be not suitable for actual sample detection.
Optical electro-chemistry sensor is based on photoelectrochemistrpool pool, using detected biomass molecule as substrate, when light sun
Pole semiconductor light-receiving aoxidizes substrate molecule according to excitation, the photohole of photoanode surface, passes through the collection realization pair of photoelectric current
The detection of biomolecule.Compared to electrochemical sensor, optical electro-chemistry sensor has higher sensitivity, because it is by separating
Light source and electrochemical workstation be composed, have the characteristics that excitaton source and electrochemical detection signal are mutually indepedent, make its survey
The photoelectric current of amount has the characteristics that signal background is low.Recently, it is sensitive without enzyme-oxygen to construct cuprous oxide film base for Li Hong wave group
Glucose optical electro-chemistry sensor (patent 201510062291.1), this sensor is to pass through detection cathode dissolution oxygen concentration
Decline carrys out the concentration of glucose in test sample, and mode is more indirect.Dong seminar has developed nanometer Au/Pt-TiO2Base non-enzymatic
Optical electro-chemistry sensor detects glucose, however, this sensor electrode preparation process is complicated and needs expensive gold/platinum nanometer
Particle.
In view of this, the present invention is specifically proposed.
Summary of the invention
The purpose of the present invention is to provide it is a kind of it is environmental-friendly, prepare simple, low in cost, high sensitivity one kind and be based on
TiO2The enzyme-free glucose optical electro-chemistry sensor of nano-stick array thin film electrode, detects the concentration of glucose.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
A kind of enzyme-free glucose optical electro-chemistry sensor, with TiO2Nano-stick array thin film electrode is working electrode, platinum filament
As to electrode, saturated calomel electrode is as reference electrode.
Enzyme-free glucose optical electro-chemistry sensor provided by the invention is that one kind is environmental-friendly, it is simple, at low cost to prepare
Honest and clean, high sensitivity one kind is based on TiO2The enzyme-free glucose optical electro-chemistry sensor of nano-stick array thin film electrode is based on light
Photoelectric current detection under excitation has mutually independent excitaton source and electrochemical signals detection system, and detection signal background is low, believes
It is number strong, without expensive instrument and equipment, just have higher detection sensitivity, and selectively good and stability is good, is Portugal
Grape sugar detection provides a simplicity, cheap new way.
Further, the TiO2Nano-stick array thin film electrode is prepared by the following method:
After conductive substrates pretreatment, butyl titanate is added and carries out hydro-thermal reaction, then annealed processing, is covered
TiO2Nano-stick array thin film electrode.
Further, the conductive substrates include FTO electro-conductive glass or ITO electro-conductive glass.
Further, the conductive substrates pretreatment are as follows: the conductive substrates are cleaned by ultrasonic, are rinsed, and in room
It is dried under temperature.
Preferably, the ultrasonic cleaning are as follows: successively use acetone, ethyl alcohol and ultrapure water to carry out ultrasound the conductive substrates
Cleaning.
Sufficiently to handle conductive substrates, good basis is provided for subsequent hydro-thermal reaction, so that obtained TiO2It receives
Rice rod array film is evenly distributed.
Further, each reagent in the hydro-thermal reaction are as follows: the volume of deionized water, hydrochloric acid solution and butyl titanate
Ratio is 15:15:0.1-1, wherein the mass concentration of the hydrochloric acid solution is 36.5%~38%.
As in various embodiments, the volume ratio of deionized water, hydrochloric acid solution and butyl titanate can be 15:
15:0.1,15:15:0.2,15:15:0.3,15:15:0.5,15:15:0.8,15:15:1 etc..
Further, it removes ionized water and hydrochloric acid solution stirs into homogeneous solution, it is anti-that butyl titanate progress hydro-thermal is added
It answers.
Specifically, the size of the conductive substrates of use is generally 2.3-3.0 centimetres of 1.8-2.5 cm x.
Each reagent in hydro-thermal reaction are as follows: take the hydrochloric acid that 15ml deionized water and 15ml mass concentration are 36.5%~38%
Homogeneous solution is stirred into, the butyl titanate that 0.1~1mL is added carries out hydro-thermal reaction.
Hydro-thermal reaction carries out in a high pressure reaction kettle, i.e., above-mentioned solution is transferred to the autoclave of 50ml, then will
Cleaned conductive substrates are inclined in reaction kettle inner wall, and then carry out hydro-thermal reaction under certain condition.
Further, the hydro-thermal reaction are as follows: reacted 120~240 minutes at 150~220 DEG C.Such as in different implementation
It can be anti-at being reacted 150 minutes, 220 DEG C at being reacted 200 minutes, 200 DEG C at being reacted 240 minutes, 180 DEG C at 150 DEG C in example
It answers 120 minutes etc..
Further, after the completion of the hydro-thermal reaction between the annealing further include: the conductive substrates are taken out,
Deionized water rinses substrate surface film, dry.
Dry purpose is the moisture for removing film surface.Room temperature naturally dry such as can be used, it can also be at 50 DEG C or so
It is dry, general dry 1h or more.
Further, the annealing are as follows: dry membrane electrode is also heat-treated 1~6h in 400~550 DEG C of temperature,
The TiO is obtained after natural cooling2Nano-stick array thin film electrode.
As in various embodiments, dry membrane electrode can be heat-treated 6h, 450 DEG C of temperature heat in 400 DEG C of temperature
Handle 3h, 500 DEG C of temperature heat treatment 3h, 550 DEG C of temperature heat treatment 1h, 500 DEG C of temperature heat treatment 2h etc..
The present invention also provides a kind of detection methods of concentration of glucose, and above-mentioned enzyme-free glucose optical electro-chemistry is sensed
Device is inserted into the electrolyte solution containing glucose, and by the enzyme-free glucose optical electro-chemistry sensor and electrochemical workstation
Connection；
Using current-vs-time measuring technology, under illumination condition, the glycoxidative photosignal of detection grape obtains grape
Sugared concentration.
The detection method of concentration of glucose provided by the invention, based on the current detecting that light excitation is lower, have excitaton source with
Electrochemical detection signal is mutually indepedent, and detection signal background is low, does not need more expensive instrument and equipment and more complex sample pretreating,
Detection sensitivity with higher provides a simplicity, cheap new way for glucose detection.
Further, the electrolyte solution includes that sulfuric acid solution, sodium hydroxide solution, potassium hydroxide solution, phosphoric acid are slow
Rush salting liquid, metabisulfite solution.
It is 0.1~1mol/L that concentration of electrolyte solutions, which generallys use concentration,.
Different electrolyte solutions uses different inclined potentiometric detections, if the concentration of NaOH solution is 1mol/L, partially electric
Position is to carry out the glycoxidative photosignal detection of grape under -0.8~-0.5V vs.SCE.
Compared with prior art, the invention has the benefit that
(1) TiO provided by the invention2The glucose optical electro-chemistry sensor of membrane electrode, based on the photoelectricity under light excitation
Stream detection has mutually independent excitaton source and electrochemical signals detection system, and detection signal background is low, signal is strong, does not have to high
Expensive instrument and equipment has higher detection sensitivity.
(2) sensor is not necessarily to the addition of enzyme preparation, greatly reduces cost, simplifies sample pretreating, enhances stability.
(3) TiO of the invention2The enzyme-free glucose optical electro-chemistry sensor and non-enzymatic noble metal base glucose of membrane electrode
Electrochemical sensor is such as: being compared, is had based on platinum, gold, copper, platinum-lead, platinum-tellurium nanoparticle enzyme-free glucose sensor
The features such as at low cost, selectivity is well and stability is good.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described.
Fig. 1 is TiO obtained in the embodiment of the present invention 12The scanning electron microscope (SEM) photograph of nano-stick array thin film electrode；
Fig. 2 is TiO obtained in the embodiment of the present invention 12The X-ray diffraction (a) and purple of nano-stick array thin film electrode
Outside-visible the spectrogram (b) that diffuses；
Fig. 3 is TiO obtained in the embodiment of the present invention 52The line of the photoelectricity oxidizing glucose of nano-stick array thin film electrode
Property scanning curve figure；
Fig. 4 is TiO obtained in the embodiment of the present invention 62The glucose optical electro-chemistry of nano-stick array thin film electrode senses
The photocurrent curve figure (a) and its calibration graph (b) that device changes with concentration of glucose；
Fig. 5 is TiO obtained in the embodiment of the present invention 72The enzyme-free glucose optical electro-chemistry of nano-stick array thin film electrode
The electrode reaction schematic diagram of sensor.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
Embodiment 1
TiO2The preparation of nano-stick array thin film electrode, includes the following steps:
(1) FTO electro-conductive glass is cut into 2 cm x, 2.5 cm size, successively using acetone, ethyl alcohol and ultrapure water to FTO
It is cleaned by ultrasonic, is then rinsed, and is dried at room temperature；
(2) it takes 15ml deionized water and 15ml hydrochloric acid (36.5%~38%) to stir into homogeneous solution, the titanium of 0.2mL is added
Solution after ten minutes, is transferred to the autoclave of 50ml, is then inclined in cleaned conductive substrates by sour four butyl esters, stirring
Conductive substrates are taken out after having reacted in reaction kettle inner wall, hydro-thermal reaction 120 minutes at 180 DEG C, and deionized water rinses substrate
Surface film, and in 50 DEG C or so dry 1h；
(3) membrane electrode of the drying obtained in step (2) is heat-treated 2h in 450 DEG C of temperature, is after natural cooling
Obtain TiO2Nano-stick array thin film electrode.
Fig. 1 is TiO in the embodiment of the present invention2Uniform nanometer rods are presented in the surface microscopic topographic of nano-stick array thin film
Array structure.
Fig. 2 is TiO obtained in the embodiment of the present invention2The X-ray diffraction (a) of nano-stick array thin film electrode and ultraviolet-
It can be seen that the spectrogram (b) that diffuses.XRD spectrum described in Fig. 2 a shows TiO obtained2Film is red schorl phase titanium dioxide.Figure
The bright TiO obtained of ultraviolet-visible diffuse reflectance spectrum chart described in 2b2Film mainly absorbs 400nm light below.
Embodiment 2
TiO2The preparation of nano-stick array thin film electrode, includes the following steps:
(1) ITO electro-conductive glass is cut into 2 cm x, 2.5 cm size, successively using acetone, ethyl alcohol and ultrapure water to ITO
It is cleaned by ultrasonic, is then rinsed, and is dried at room temperature；
(2) it takes 15ml deionized water and 15ml hydrochloric acid (36.5%~38%) to stir into homogeneous solution, the titanium of 0.1mL is added
Solution after five minutes, is transferred to the autoclave of 50ml, is then inclined in cleaned conductive substrates by sour four butyl esters, stirring
Conductive substrates are taken out after having reacted in reaction kettle inner wall, hydro-thermal reaction 240 minutes at 150 DEG C, and deionized water rinses substrate
Surface film, and in 50 DEG C ± 5 DEG C dry 1h；
(3) membrane electrode of the drying obtained in step (2) is heat-treated 6h in 400 DEG C of temperature, is after natural cooling
Obtain TiO2Nano-stick array thin film electrode.
Through detecting, TiO in the embodiment of the present invention2The surface microscopic topographic of nano-stick array thin film electrode is presented uniform
Nanometer stick array structure.
It is detected through X-ray diffraction and UV-Vis DRS, shows TiO obtained2Film is Rutile Type titanium dioxide
Titanium；The bright TiO obtained of ultraviolet-visible diffuse reflectance spectrum chart2Film mainly absorbs 400nm light below.
Embodiment 3
TiO2The preparation of nano-stick array thin film electrode, includes the following steps:
(1) FTO electro-conductive glass is cut into 2 cm x, 2.5 cm size, successively using acetone, ethyl alcohol and ultrapure water to FTO
It is cleaned by ultrasonic, is then rinsed, and is dried at room temperature；
(2) it takes 15ml deionized water and 15ml hydrochloric acid (36.5%~38%) to stir into homogeneous solution, the metatitanic acid of 1mL is added
Solution is transferred to the autoclave of 50ml, is then inclined in cleaned conductive substrates instead by four butyl esters after stirring 15 minutes
Kettle inner wall is answered, conductive substrates are taken out after having reacted in hydro-thermal reaction 120 minutes at 220 DEG C, and deionized water rinses substrate table
Face film, and in 50 DEG C or so dry 1h；
(3) membrane electrode of the drying obtained in step (2) is heat-treated 1h in 550 DEG C of temperature, is after natural cooling
Obtain TiO2Nano-stick array thin film electrode.
Through detecting, TiO in the embodiment of the present invention2The surface microscopic topographic of nano-stick array thin film electrode is presented uniform
Nanometer stick array structure.
It is detected through X-ray diffraction and UV-Vis DRS, shows TiO obtained2Film is Rutile Type titanium dioxide
Titanium；The bright TiO obtained of ultraviolet-visible diffuse reflectance spectrum chart2Film mainly absorbs 400nm light below.
Embodiment 4
TiO2The preparation of nano-stick array thin film electrode, includes the following steps:
(1) FTO electro-conductive glass is cut into 2 cm x, 2.5 cm size, successively using acetone, ethyl alcohol and ultrapure water to FTO
It is cleaned by ultrasonic, is then rinsed, and is dried at room temperature；
(2) it takes 15ml deionized water and 15ml hydrochloric acid (36.5%~38%) to stir into homogeneous solution, the titanium of 0.5mL is added
Solution after ten minutes, is transferred to the autoclave of 50ml, is then inclined in cleaned conductive substrates by sour four butyl esters, stirring
Conductive substrates are taken out after having reacted in reaction kettle inner wall, hydro-thermal reaction 150 minutes at 180 DEG C, and deionized water rinses substrate
Surface film, and in 50 DEG C or so dry 2h；
(3) membrane electrode of the drying obtained in step (2) is heat-treated 3h in 500 DEG C of temperature, is after natural cooling
Obtain TiO2Nano-stick array thin film electrode.
Through detecting, TiO in the embodiment of the present invention2The surface microscopic topographic of nano-stick array thin film electrode is presented uniform
Nanometer stick array structure.
It is detected through X-ray diffraction and UV-Vis DRS, shows TiO obtained2Film is Rutile Type titanium dioxide
Titanium；The bright TiO obtained of ultraviolet-visible diffuse reflectance spectrum chart2Film mainly absorbs 400nm light below.
Embodiment 5
By TiO made from embodiment 12Nano-stick array thin film electrode is sweet to electrode, saturation as working electrode, Pt piece
Mercury electrode SCE is reference electrode, and three-electrode system is connect with electrochemical workstation；Using AM1.5G solar simulator as light
Source (light intensity 100mW/cm2), in the NaOH electrolyte solution of 1mol/L, linearly sweeping when test is added or is added without glucose
Curve is retouched, test result is shown in Fig. 3.
Fig. 3 shows to be added the photoelectric current after low-down glucose (concentration 0.4mmol/L) and is apparently higher than in blank
The photoelectric current surveyed in NaOH electrolyte, illustrates TiO2Ability with photoelectrocatalysioxidization oxidization glucose.
Similarly, TiO made from embodiment 2-42Nano-stick array thin film electrode is examined in the same way with embodiment 1
It surveys, TiO made from embodiment 2-42Nano-stick array thin film electrode is to electrode, saturation calomel electricity as working electrode, Pt piece
Pole SCE is reference electrode, and the photoelectric current after the low-down glucose (concentration 0.4mmol/L) of detection addition is apparently higher than in sky
The photoelectric current surveyed in white NaOH electrolyte, equally illustrates TiO2Ability with photoelectrocatalysioxidization oxidization glucose.
Embodiment 6
The detection method of concentration of glucose, steps are as follows:
(1) by TiO made from embodiment 12Nano-stick array thin film electrode is used as working electrode, platinum filament to electrode,
Calomel is saturated as reference electrode, establishes three-electrode system, i.e., enzyme-free glucose optical electro-chemistry sensor provided by the invention will
Three electrodes are inserted into the NaOH solution of the 1mol/L containing a certain concentration glucose, and by three-electrode system and electrochemical workstation
Connection；
(2) using the three-electrode system in step (1), using current-vs-time measuring technology, inclined current potential is -0.65V
Under vs.SCE, the glycoxidative photosignal detection of grape is carried out under the irradiation of simulated solar irradiation.
(3) according to the calibration curve of glucose substrate concentration and detection photoelectric current, containing for glucose in solutions to be measured is measured
Amount.
Fig. 4 a is TiO of the present invention2The glucose optical electro-chemistry sensor of nano-stick array thin film electrode is with concentration of glucose
The photocurrent curve figure of variation.In Fig. 4 a, the lines that the curve on the left side is indicated with the arrow on the right are corresponding step by step.
Fig. 4 a testing result shows that, with the increase of concentration of glucose, photoelectric current is obviously at increase tendency.
Fig. 4 b is photoelectric current-concentration calibration curve, the results showed that photoelectric current is 0mM~0.2mM model in concentration of glucose
Enclose interior linear, corresponding detection sensitivity is respectively 201.5uAcm-2·mM-1.Prove the optical electro-chemistry sensor
There is higher sensitivity.
In addition, TiO made from embodiment 2-42Nano-stick array thin film has consistent detection glucose dense with embodiment 1
The effect of degree.
Embodiment 7
TiO provided by the invention2The detection mechanism of the glucose optical electro-chemistry sensor of nano-stick array thin film electrode is such as
Shown in Fig. 5.
Fig. 5 is TiO of the present invention2The electrode reaction of the glucose optical electro-chemistry sensor of nano-stick array thin film electrode is illustrated
Figure, the glucose optical electro-chemistry sensor is with TiO2Membrane electrode is used as working electrode, platinum filament to electrode, is saturated calomel and is made
For reference electrode.Light excites TiO2Semiconductor generates electrons and holes, and hole migration to electrode surface is glycoxidative by grape, electronics
It is flowed to via external circuits and current loop is formed to electrode, photo-signal is obtained, according to the calibration of photoelectric current-concentration of glucose
Curve detects concentration of glucose.
In summary, enzyme-free glucose optical electro-chemistry sensor provided by the invention, based on the photoelectric current inspection under light excitation
It surveys, there is mutually independent excitaton source and electrochemical signals detection system, detection signal background is low, signal is strong, without valuableness
Instrument and equipment just has higher detection sensitivity；Sensor is not necessarily to the addition of enzyme preparation, greatly reduces cost, simplifies
Sample pretreating, enhances stability；Compared with the enzyme-free glucose sensor of other nanoparticles, have it is at low cost,
The features such as selectivity is good and stability is good.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from of the invention
Many other change and modification can be made in the case where spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of enzyme-free glucose optical electro-chemistry sensor, which is characterized in that with TiO2Nano-stick array thin film electrode is work electricity
Pole, platinum filament are used as to electrode, and saturated calomel electrode is as reference electrode.
2. enzyme-free glucose optical electro-chemistry sensor according to claim 1, which is characterized in that the TiO2Nanometer rods battle array
Column membrane electrode is prepared by the following method:
After conductive substrates pretreatment, butyl titanate is added and carries out hydro-thermal reaction, then annealed processing obtains covering TiO2It receives
Rice rod array film electrode.
3. enzyme-free glucose optical electro-chemistry sensor according to claim 2, which is characterized in that the conductive substrates include
FTO electro-conductive glass or ITO electro-conductive glass.
4. enzyme-free glucose optical electro-chemistry sensor according to claim 2, which is characterized in that the conductive substrates are located in advance
Reason are as follows: the conductive substrates are cleaned by ultrasonic, are rinsed, and are dried at room temperature；
Preferably, the ultrasonic cleaning are as follows: successively use acetone, ethyl alcohol and ultrapure water to carry out ultrasound the conductive substrates clear
It washes.
5. enzyme-free glucose optical electro-chemistry sensor according to claim 2, which is characterized in that in the hydro-thermal reaction
Each reagent are as follows: the volume ratio of deionized water, hydrochloric acid solution and butyl titanate is 15:15:0.1-1, wherein the hydrochloric acid is molten
The mass concentration of liquid is 36.5%~38%；
Further, it removes ionized water and hydrochloric acid solution stirs into homogeneous solution, butyl titanate is added and carries out hydro-thermal reaction.
6. enzyme-free glucose optical electro-chemistry sensor according to claim 5, which is characterized in that the hydro-thermal reaction are as follows:
It is reacted 120~240 minutes at 150~220 DEG C.
7. according to the described in any item enzyme-free glucose optical electro-chemistry sensors of claim 2-6, which is characterized in that the hydro-thermal
After the reaction was completed between the annealing further include: the conductive substrates are taken out, deionized water rinses substrate surface film,
It is dry.
8. enzyme-free glucose optical electro-chemistry sensor according to claim 7, which is characterized in that the annealing are as follows:
Dry membrane electrode is also heat-treated 1~6h in 400~550 DEG C of temperature, obtains the TiO after natural cooling2Nanometer rods
Array film electrode.
9. a kind of detection method of concentration of glucose, which is characterized in that by the described in any item enzyme-free glucoses of claim 1-8
Optical electro-chemistry sensor be inserted into the electrolyte solution containing glucose in, and by the enzyme-free glucose optical electro-chemistry sensor with
Electrochemical workstation connection；
Using current-vs-time measuring technology, under illumination condition, it is dense to obtain glucose for the glycoxidative photosignal of detection grape
Degree.
10. detection method according to claim 9, which is characterized in that the electrolyte solution includes sulfuric acid solution, hydrogen-oxygen
Change sodium solution, potassium hydroxide solution, phosphate buffered saline solution, metabisulfite solution.
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| CN110823973A (en) * | 2019-11-21 | 2020-02-21 | 湖北工程学院 | Self-powered photoelectrochemistry enzyme-free glucose sensor and preparation method and detection method thereof |
| CN110823972A (en) * | 2019-11-21 | 2020-02-21 | 湖北工程学院 | Based on Fe2O3Enzyme-free glucose photoelectrochemical sensor of electrode and preparation method and detection method thereof |
| CN112763424A (en) * | 2020-12-09 | 2021-05-07 | 上海大学 | Enzyme-free glucose detection sensor and manufacturing method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110823973A (en) * | 2019-11-21 | 2020-02-21 | 湖北工程学院 | Self-powered photoelectrochemistry enzyme-free glucose sensor and preparation method and detection method thereof |
| CN110823972A (en) * | 2019-11-21 | 2020-02-21 | 湖北工程学院 | Based on Fe2O3Enzyme-free glucose photoelectrochemical sensor of electrode and preparation method and detection method thereof |
| CN110823972B (en) * | 2019-11-21 | 2022-08-09 | 湖北工程学院 | Based on Fe 2 O 3 Enzyme-free glucose photoelectrochemical sensor of electrode and preparation method and detection method thereof |
| CN110823973B (en) * | 2019-11-21 | 2022-08-09 | 湖北工程学院 | Self-powered photoelectrochemistry enzyme-free glucose sensor and preparation method and detection method thereof |
| CN112763424A (en) * | 2020-12-09 | 2021-05-07 | 上海大学 | Enzyme-free glucose detection sensor and manufacturing method thereof |
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