CN212059886U

CN212059886U - Mueller matrix polarization imaging system for pathology detection

Info

Publication number: CN212059886U
Application number: CN202020515063.1U
Authority: CN
Inventors: 翟石磊; 何轶兵; 杜慧林; 孙安玉; 解青鹏
Original assignee: Wuhan Wanping Electronic Technology Co ltd
Current assignee: Wuhan Wanping Electronic Technology Co ltd
Priority date: 2020-04-10
Filing date: 2020-04-10
Publication date: 2020-12-01
Anticipated expiration: 2030-04-10

Abstract

The utility model discloses a muller matrix polarization imaging system for pathology testing. The device comprises a camera imaging module, a light source module, a polarizing module, a polarization analyzing module, an objective table module and a reflector module. The camera imaging module comprises a camera, a lens and a microscope objective. The polarizing module comprises a polarizing plate, a wave plate and an annular ultrasonic motor. The polarization analyzing module comprises a polarizing plate, a wave plate and an annular ultrasonic motor. The light source generates light which sequentially passes through the polarizing module, the reflector module, the objective table module, the polarization analyzing module and the camera imaging module, and finally the camera module photographs and images the slice at different angles of rotation of the wave plate. The utility model discloses have compact small and exquisite and handy mechanism, and polarized light modulation is accurate, and the scene that is applicable to pathological examination uses.

Description

Mueller matrix polarization imaging system for pathology detection

Technical Field

The utility model belongs to the pathology testing field, concretely relates to muller matrix polarization imaging system for pathology testing. The utility model is used for the method of muller matrix polarization imaging carries out pathological section and detects, can provide polarization information for biological tissue.

Background

In the current clinical diagnosis of cancer, doctors detect cancer by observing the cell morphology, the cell nucleus size and other pathological manifestations related to canceration of stained tissue sections under a microscope, but cannot acquire the polarization information of pathological tissue sections. When the tissue is cancerated, the cells can generate specific pathological changes, such as the morphological change of the cancerated cells, the cell nucleus becomes larger, the cancer cells are denser, and the physiological and pathological changes of the microstructure can cause the change of the polarization information of the tissue. Since the scattered light of the tissue carries a large amount of polarization information, the structure of the tissue can be inverted by detecting the cancerous tissue by using a polarization imaging method and measuring and analyzing the polarization state of the scattered light of the corresponding tissue.

The development of a polarization imaging detection device based on the mueller matrix provides a feasible path for solving the problems. For the polarization characteristic of an object, the most complete expression mode is characterized by adopting a Mueller matrix, the Mueller matrix is a mathematical description method which covers all polarization information of the target, and the Mueller matrix contains abundant microstructure and optical information of a sample and can fully express the polarization matrix characteristic of the target. Therefore, the polarization imaging technology is used for detecting the cancerous tissues, the Mueller matrix measurement is carried out on the pathological tissues, the polarization characteristics of the pathological tissues before and after canceration are detected, the frequently ignored pathological information is obtained, the detection of the cancerous tissues is very meaningful, and a new reference index can be provided for clinical pathological diagnosis. Although research on the Mueller matrix polarization imaging technology has been conducted by relevant research and development teams at home and abroad as early as a decade ago, mature equipment for pathological detection has not been on the market.

Disclosure of Invention

The utility model aims at providing a muller matrix polarization imaging system for pathological examination to pathological examination's application demand among the medical institution.

In order to achieve the above purpose, the utility model adopts the following technical scheme to solve:

the utility model comprises a camera imaging module, a light source module, a polarizing module, a polarization detecting module, an objective table and a reflector module; the camera imaging module comprises a camera, a lens and a microscope objective; the camera is arranged at one side far away from the polarization analysis module, the microscope objective is arranged at one side close to the polarization analysis module, and the lens is arranged between the camera and the microscope objective; the polarizing module comprises a polarizer I, a wave plate I and an annular ultrasonic motor I; the polaroid I is arranged on one side close to the light source module, and the wave plate I is arranged on one side far away from the light source module, is carried by the annular ultrasonic motor I arranged in the middle and rotates at a fixed angle; the polarization analyzing module comprises a polarizing film II, a wave plate II and an annular ultrasonic motor II; the polaroid II is arranged on one side close to the camera imaging module, and the wave plate II is arranged on one side far away from the camera imaging module and is carried by an annular ultrasonic motor II arranged in the middle and rotates at a fixed angle;

the light source in the light source module generates illumination which sequentially passes through the polarizing module, the reflector module, the objective table, the polarization analyzing module and the camera imaging module, and finally the camera imaging module photographs and images the slice under the rotation of the wave plate at different angles.

The utility model controls the annular ultrasonic motor I and the annular ultrasonic motor II in the polarization module and the polarization detection module to respectively rotate the wave plate I and the wave plate II controlled by the two motors by a fixed angle; and after the wave plate rotates to a set angle, the wave plate stops rotating to wait for the camera imaging module to shoot and image, and after the camera imaging module shoots, the annular ultrasonic motor continues rotating.

The rotation angle of the two wave plates can be set to be 5:1 or 10: 1.

The reflector module consists of a reflector and a reflector rotating shaft, and the light path reflection path can be adjusted by rotating and adjusting the angle of the reflector.

The objective table is used for fixing pathological sections, and the objective table can be finely adjusted along the three directions of an X axis, a Y axis and a Z axis.

The utility model discloses beneficial effect as follows:

based on the above scheme, the utility model discloses an optimize light path, refine motion, perfect integrated design, the imaging system based on the mueller matrix polarization imaging principle invention carries out the mueller matrix to pathological mechanism and measures, surveys its polarization characteristic in order to realize the detection purpose of supplementary canceration tissue, and equipment structure is succinct, polarization modulation is accurate stable, be fit for the advantage that multiple pathological section detected, is fit for carrying out pathological examination in medical institution.

Drawings

Fig. 1 is a block diagram of a mueller matrix polarization imaging system for pathology testing according to the present invention;

FIG. 2 is a schematic diagram of the polarization module of the present invention (the same applies to the polarization detection module);

the reference numbers in the figures illustrate: 1-light source module, 2-polarizing module, 3-reflector module, 4-camera imaging module, 5-polarization analysis module, 6-objective table, 101-light source heat dissipation shell, 201-polarizer I, 202-annular ultrasonic motor I, 203-wave plate I, 301-reflector, 302-reflector rotating shaft, 401-camera, 402-lens, 403-objective lens, 501-polarizer II, 502-annular ultrasonic motor II, 503-wave plate II, 601-pathological section

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and examples.

The utility model aims at providing a muller matrix polarization imaging system for pathological examination to pathological examination's application demand among the medical institution, the utility model discloses utensil equipment structure is succinct, the accurate stability of polarization modulation, be fit for the advantage that multiple pathological section detected, is fit for carrying out the pathological examination requirement in medical institution.

As shown in fig. 1, a mueller matrix polarization imaging system for pathology detection includes a camera imaging module 4, a light source module 1, a polarizing module 2, an analyzing module 5, an object stage 6, and a mirror module 3. The camera imaging module 4 includes a camera 401, a lens 402, and a microscope objective 403. The polarizing module 2 comprises a polarizing plate 201, a wave plate 203 and a ring-shaped ultrasonic motor 202. The polarization analyzing module 5 comprises a polarization plate 501, a wave plate 503 and a ring-shaped ultrasonic motor 502. The light source 1 generates light which sequentially passes through the polarizing module 2, the reflector module 3, the objective table 6, the polarization analyzing module 5 and the camera imaging module 4, and finally the camera imaging module 4 photographs and images the slice 601 under the condition that the wave plate 203 and the wave plate 503 rotate to different angles.

As shown in fig. 2, the polarization module 2 includes a polarizer 201, a wave plate 203, and a ring-shaped ultrasonic motor 202, the polarizer 201 is disposed on a side close to the light source module 1, and the wave plate 203 is disposed on a side far from the light source module 1 and is carried by the ring-shaped ultrasonic motor 202 and rotates at a fixed angle. The polarization analysis module 5 comprises a polarizer 501, a wave plate 503 and an annular ultrasonic motor 502, wherein the polarizer 501 is arranged on one side close to the camera module 4, and the wave plate 503 is arranged on one side far away from the camera module 4, is carried by the annular ultrasonic motor 502 and rotates at a fixed angle.

As shown in fig. 1, the two wave plates 203 and 503 are rotated by a fixed angle by controlling the annular

ultrasonic motors

202 and 502 in the polarization module 2 and the polarization analyzing module 5, the rotation angles of the two wave plates 203 and 503 can be set to 5:1 or 10:1, the rotation of the wave plates is suspended after the rotation of the wave plates reaches the set angle, the camera module 4 is waited for imaging by taking a picture, and the annular

ultrasonic motors

202 and 502 continue to rotate after the camera imaging module 4 takes a picture.

The light of the imaging system is provided by the light source module 1, and a light source heat dissipation shell 101 is arranged outside the light source module 1; the light is reflected by the reflector module 3, and the reflector module 3 can rotate around the reflector rotating shaft 302 and fix the angle to adjust the reflection path of the light path. The object stage 6 fixes the pathological section 601, and the object stage 6 has fine adjustment along three directions of an X axis, a Y axis and a Z axis.

The above-mentioned embodiments are merely a preferred embodiment of the present invention, but it is not intended to limit the present invention. Various changes and modifications can be made by one of ordinary skill in the pertinent art without departing from the spirit and scope of the present invention. Therefore, all the technical schemes obtained by adopting the mode of equivalent replacement or equivalent transformation fall within the protection scope of the utility model.

Claims

1. The Mueller matrix polarization imaging system for pathological detection comprises a camera imaging module, a light source module, a polarizing module, an analyzing module, an objective table and a reflector module; the camera imaging module comprises a camera, a lens and a microscope objective; the camera is arranged at one side far away from the polarization analysis module, the microscope objective is arranged at one side close to the polarization analysis module, and the lens is arranged between the camera and the microscope objective; the polarizing module comprises a polarizer I, a wave plate I and an annular ultrasonic motor I; the polaroid I is arranged on one side close to the light source module, and the wave plate I is arranged on one side far away from the light source module, is carried by the annular ultrasonic motor I arranged in the middle and rotates at a fixed angle; the polarization analyzing module comprises a polarizing film II, a wave plate II and an annular ultrasonic motor II; the polaroid II is arranged on one side close to the camera imaging module, and the wave plate II is arranged on one side far away from the camera imaging module and is carried by an annular ultrasonic motor II arranged in the middle and rotates at a fixed angle;

2. The mueller matrix polarization imaging system for pathology inspection according to claim 1, wherein by controlling an annular ultrasonic motor i and an annular ultrasonic motor ii in the polarization module and the polarization analyzing module, the wave plate i and the wave plate ii controlled by the two motors are respectively rotated by a fixed angle; and after the wave plate rotates to a set angle, the wave plate stops rotating to wait for the camera imaging module to shoot and image, and after the camera imaging module shoots, the annular ultrasonic motor continues rotating.

3. The mueller matrix polarization imaging system for pathology detection according to claim 1 or 2, wherein the rotation angles of the two wave plates are set to 5:1 or 10: 1.

4. The mueller matrix polarization imaging system for pathology detection according to claim 3, wherein the mirror module comprises a mirror and a mirror rotating shaft, and the light path reflection path can be adjusted by rotating and adjusting the angle of the mirror.

5. The mueller matrix polarization imaging system for pathology detection of claim 4, wherein the stage is configured to hold a pathological section, and wherein the stage is capable of fine adjustment along three directions, namely, X-axis, Y-axis, and Z-axis.