CN202599807U - Sperm motility detection device - Google Patents
Sperm motility detection device Download PDFInfo
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- CN202599807U CN202599807U CN 201220297928 CN201220297928U CN202599807U CN 202599807 U CN202599807 U CN 202599807U CN 201220297928 CN201220297928 CN 201220297928 CN 201220297928 U CN201220297928 U CN 201220297928U CN 202599807 U CN202599807 U CN 202599807U
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- sperm motility
- objective table
- driving governor
- pick
- control computer
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- 230000019100 sperm motility Effects 0.000 title claims abstract description 43
- 238000001514 detection method Methods 0.000 title abstract description 17
- 238000005070 sampling Methods 0.000 claims description 4
- 102100021935 C-C motif chemokine 26 Human genes 0.000 claims description 3
- 101000897493 Homo sapiens C-C motif chemokine 26 Proteins 0.000 claims description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical group [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 3
- 229910052753 mercury Inorganic materials 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000003287 optical effect Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 5
- 230000002950 deficient Effects 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000004445 quantitative analysis Methods 0.000 description 3
- 238000001000 micrograph Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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Abstract
The utility model provides a sperm motility detection device, which comprises a microscope, a microscope camera, a control computer and an objective table used for supporting sperm slides; an objective of the microscope is placed above the objective table; the microscope camera is arranged at an output optical path of the microscope; and an output end of the microscope camera is connected with the control computer. According to the utility model, a computer technology is adopted to analyze the sperm motility in an auxiliary way, so that the sperm motility detection device has the advantages of fast detection speed, good repeatability, high data reliability and comprehensive detection indexes.
Description
Technical field
The utility model belongs to field of computer technology, is specifically related to a kind of sperm motility pick-up unit.
Background technology
Along with the continuous development of society, become human research's a hot fields already about the research of prenatal and postnatal care aspect, wherein, occupy bigger proportion in the research of the detection of mankind spermatozoon quality aspect prenatal and postnatal care.
Medical research shows that two core technology indexs weighing the mankind spermatozoon quality are sperm motility and sperm morphology.At present; The mankind spermatozoon quality determining method that hospital generally adopts is: at first sperm is made into test slides, then test slides is placed on the objective table central position, with microscopical objective lens alignment test slide; Experienced doctor is through the microscopical eyepiece motion conditions of Direct observation sperm with the naked eye; Information such as movement rate with doctor's experience record sperm contrast relevant criterion then, confirm whether the vigor performance of sperm is qualified.
Mainly there is following defective in above-mentioned sperm motility detection method: (one) needs doctor's observation of rich experiences, and usable range has certain limitation; And testing result depends on doctor's subjective judgement, has the shortcoming of poor repeatability; (2) detection time is long: detect the motion conditions that 1 sample need be observed 400 sperms usually, detection time was above 30 minutes; (3) testing result is a qualitative results, can only draw move about qualitative results such as speed is fast, slow or motionless of sperm, can not be quantitative, so have the low defective of accuracy of detection; (4) technical indicator that detects is single, can not analyze the sperm motility situation comprehensively.
The utility model content
To the defective that prior art exists, the utility model provides a kind of sperm motility pick-up unit, adopts computer technology assistant analysis sperm motility, but has that detection speed is fast, good reproducibility, data property degree is high and detect the comprehensive advantage of index.
The technical scheme that the utility model adopts is following:
The utility model provides a kind of sperm motility pick-up unit, comprising: microscope, microimaging machine, control computer and be used to support the objective table of sperm slide; Said microscopical object lens are positioned at the top of said objective table, and said microimaging machine is arranged on said microscopical output light path place, and the output terminal of said microimaging machine is connected with said control computer.
Preferably, said microimaging machine is that ccd video camera or cmos camera and/or said microscope are that Olympus CX41 phase microscope and/or said control computer are apple IMAC integral type computing machine.
Preferably, said microimaging machine for the image resolution ratio of gathering more than 782 * 582, and the microimaging machine of sampling frame per second more than 30fps.
Preferably, said microimaging machine is connected through the IEEE1394b line with said control computer.
Preferably, also comprise: objective table mobile controller and driving governor; The output terminal of said objective table mobile controller is connected with said objective table, and the input end of said objective table mobile controller is connected with the output terminal of said driving governor, and the input end of said driving governor is connected with the output terminal of said control computer.
Preferably, said objective table mobile controller comprises X shaft step motor and y-axis stepper motor; Said driving governor comprises X axle driving governor and Y axle driving governor; Said X shaft step motor is connected with said control computer through said X axle driving governor, and said y-axis stepper motor is connected with said control computer through said Y axle driving governor.
Preferably, said objective table mobile controller comprises the Z shaft step motor; Said driving governor comprises Z axle driving governor; Said Z shaft step motor is connected with said control computer through said Z axle driving governor.
Preferably, also comprise: be used for steady light source to said objective table irradiates light.
Preferably, said steady light source is mercury lamp light source or infrared steady light source or the ultraviolet steady light source of power greater than 30w.
Preferably, also comprise: green filter and rotating disk condenser with annular diaphragm; Said green filter is arranged on said steady light source in the light path of said sperm slide, and said rotating disk condenser is arranged on said green filter in the light path of said sperm slide.
The beneficial effect of the utility model is following:
The sperm motility pick-up unit that the utility model provides; The micro-enlarged and displayed sperm motility of microscope image at first; The sperm motility image that shows of camera acquisition microscope then, and this sperm motility image is transferred to computing machine, through preset software the sperm motility image is carried out quantitative analysis by computing machine; Obtain the sperm motility situation, but have that detection speed is fast, good reproducibility, data property degree is high and detect the comprehensive advantage of index.
Description of drawings
The structural representation of the sperm motility pick-up unit that Fig. 1 provides for the utility model;
Wherein, 1---microscope; 2---microimaging machine; The 3---control computer; 4---sperm slide; The microscopical object lens of 5---.
Embodiment
Below in conjunction with accompanying drawing the utility model is elaborated:
As shown in Figure 1, the utility model provides a kind of sperm motility pick-up unit, comprising: microscope, microimaging machine, control computer and be used to support the objective table of sperm slide; Said microscopical object lens are positioned at the top of said objective table, and said microimaging machine is arranged on said microscopical output light path place, and the output terminal of said microimaging machine is connected with said control computer.For improving accuracy of detection, the microimaging machine can be ccd video camera or cmos camera, and the image resolution ratio that more specifically is collection is more than 782 * 582, and the sampling frame per second is at the above microimaging machine of 30fps (frames per second, frame/second).Microscope is an Olympus CX41 phase microscope, and control computer is an apple IMAC integral type computing machine.The microimaging machine can be connected through the IEEE1394b line with control computer, thereby realizes communicating by letter between microimaging machine and the control computer.
In addition, for improving accuracy of detection, in the utility model, moving of objective table can be controlled by control computer, and be concrete: as also to comprise: objective table mobile controller and driving governor; The output terminal of said objective table mobile controller is connected with said objective table, and the input end of said objective table mobile controller is connected with the output terminal of said driving governor, and the input end of said driving governor is connected with the output terminal of said control computer.The objective table mobile controller can be realized objective table moving along X axle and Y direction; Also can realize objective table along the moving of Z-direction, below introduce both of these case respectively: (one) realizes objective table mobile along X axle and Y direction: the objective table mobile controller comprises X shaft step motor and y-axis stepper motor; Said driving governor comprises X axle driving governor and Y axle driving governor; Said X shaft step motor is connected with said control computer through said X axle driving governor, and said y-axis stepper motor is connected with said control computer through said Y axle driving governor.(2) realize objective table moving along Z-direction: the objective table mobile controller comprises the Z shaft step motor; Said driving governor comprises Z axle driving governor; Said Z shaft step motor is connected with said control computer through said Z axle driving governor.Certainly, also can above-mentioned (one) (two) be combined, realize objective table moving simultaneously along X axle, Y axle and three directions of Z axle.
In addition, in the utility model, can also comprise the steady light source that is used for to said objective table irradiates light, thereby improve accuracy of detection.Concrete, the type of steady light source can be the mercury lamp light source of power greater than 30w, or infrared steady light source or the steady light source of ultraviolet.Also comprise: green filter and rotating disk condenser with annular diaphragm; Said green filter is arranged on said steady light source in the light path of said sperm slide, and said rotating disk condenser is arranged on said green filter in the light path of said sperm slide.Therefore, after the light that sends from steady light source at first filters through green filter, through the rotating disk condenser light is converged the back according to the sperm slide again.
The principle of work of the sperm motility pick-up unit that the utility model provides is: the sperm slide is processed with sperm to be detected in (1), and is placed on the objective table central position; (2) open steady light source, to sperm slide irradiates light; (3) through the sperm motility image in the microscope enlarged and displayed sperm slide, wherein, enlargement factor is 200～400 times; (4) the sperm motility image that shows of camera acquisition microscope, and this sperm motility image is transferred to computing machine; Wherein, the image resolution ratio of camera acquisition is more than 782 * 582, more than the sampling frame per second 30fps; (5) computing machine carries out quantitative analysis through preset software to the sperm motility image, obtains sperm motility correlation technique desired value; (6) printing step (5) detects the sperm motility correlation technique desired value that obtains, and accomplishes and detects.
In sum; The sperm motility pick-up unit that the utility model provides, the at first micro-enlarged and displayed sperm motility of microscope image, the sperm motility image of camera acquisition microscope demonstration then; And this sperm motility image is transferred to computing machine; Through preset software the sperm motility image is carried out quantitative analysis by computing machine, obtain the sperm motility situation, but have that detection speed is fast, detection efficiency is high, good reproducibility, data property degree is high and detect the comprehensive advantage of index.
The above only is the preferred implementation of the utility model; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the utility model principle; Can also make some improvement and retouching, these improvement and retouching also should be looked the protection domain of the utility model.
Claims (10)
1. a sperm motility pick-up unit is characterized in that, comprising: microscope, microimaging machine, control computer and be used to support the objective table of sperm slide; Said microscopical object lens are positioned at the top of said objective table, and said microimaging machine is arranged on said microscopical output light path place, and the output terminal of said microimaging machine is connected with said control computer.
2. sperm motility pick-up unit according to claim 1; It is characterized in that said microimaging machine is that ccd video camera or cmos camera and/or said microscope are that Olympus CX41 phase microscope and/or said control computer are apple IMAC integral type computing machine.
3. sperm motility pick-up unit according to claim 2 is characterized in that, said microimaging machine for the image resolution ratio of gathering more than 782 * 582, and the microimaging machine of sampling frame per second more than 30fps.
4. sperm motility pick-up unit according to claim 1 is characterized in that, said microimaging machine is connected through the IEEE1394b line with said control computer.
5. sperm motility pick-up unit according to claim 1 is characterized in that, also comprises: objective table mobile controller and driving governor; The output terminal of said objective table mobile controller is connected with said objective table, and the input end of said objective table mobile controller is connected with the output terminal of said driving governor, and the input end of said driving governor is connected with the output terminal of said control computer.
6. sperm motility pick-up unit according to claim 5 is characterized in that, said objective table mobile controller comprises X shaft step motor and y-axis stepper motor; Said driving governor comprises X axle driving governor and Y axle driving governor; Said X shaft step motor is connected with said control computer through said X axle driving governor, and said y-axis stepper motor is connected with said control computer through said Y axle driving governor.
7. sperm motility pick-up unit according to claim 5 is characterized in that, said objective table mobile controller comprises the Z shaft step motor; Said driving governor comprises Z axle driving governor; Said Z shaft step motor is connected with said control computer through said Z axle driving governor.
8. sperm motility pick-up unit according to claim 1 is characterized in that, also comprises: be used for the steady light source to said objective table irradiates light.
9. sperm motility pick-up unit according to claim 8 is characterized in that, said steady light source is mercury lamp light source or infrared steady light source or the ultraviolet steady light source of power greater than 30w.
10. sperm motility pick-up unit according to claim 8 is characterized in that, also comprises: green filter and the rotating disk condenser with annular diaphragm; Said green filter is arranged on said steady light source in the light path of said sperm slide, and said rotating disk condenser is arranged on said green filter in the light path of said sperm slide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220297928 CN202599807U (en) | 2012-06-19 | 2012-06-19 | Sperm motility detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220297928 CN202599807U (en) | 2012-06-19 | 2012-06-19 | Sperm motility detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202599807U true CN202599807U (en) | 2012-12-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220297928 Expired - Fee Related CN202599807U (en) | 2012-06-19 | 2012-06-19 | Sperm motility detection device |
Country Status (1)
| Country | Link |
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| CN (1) | CN202599807U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104237121A (en) * | 2014-09-18 | 2014-12-24 | 山东大学 | Sperm motility performance detection method |
-
2012
- 2012-06-19 CN CN 201220297928 patent/CN202599807U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104237121A (en) * | 2014-09-18 | 2014-12-24 | 山东大学 | Sperm motility performance detection method |
| CN104237121B (en) * | 2014-09-18 | 2016-06-01 | 山东大学 | A kind of motility of sperm method for testing performance |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121212 Termination date: 20190619 |