CN203894149U - Supermicron fluorescence sperm morphology multifunctional detection and analysis system - Google Patents
Supermicron fluorescence sperm morphology multifunctional detection and analysis system Download PDFInfo
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- CN203894149U CN203894149U CN201420185062.XU CN201420185062U CN203894149U CN 203894149 U CN203894149 U CN 203894149U CN 201420185062 U CN201420185062 U CN 201420185062U CN 203894149 U CN203894149 U CN 203894149U
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Abstract
The utility model relates to a supermicron fluorescence sperm morphology multifunctional detection and analysis system, which belongs to the technical field of microorganism analysis and detection. The supermicron fluorescence sperm morphology multifunctional detection and analysis system comprises a main body, a microcomputer controller and a computer; the main body comprises a high-definition camera, an optical microscope and a fluorescence device, the high-definition camera consists of a camera and an optical amplification zoom lens, the optical microscope consists of an objective lens, an ocular lens, a movable worktable, a sperm counting tank, a two-dimensional adjusting mechanism of the worktable and a foundation support, and the fluorescence device consists of a fluorescence light box, a fluorescence splitter and a fluorescence power source. The supermicron fluorescence sperm morphology multifunctional detection and analysis system is strong in function and convenient to use and has the advantages of simple structure, high integration level, small size, strong function, easiness in operation and the like.
Description
Technical field
The utility model belongs to microbiological analysis detection technique field, particularly TH-ultra micro, fluorescence sperm morphology multifunctional examining cls analysis system.
Technical background
Up-to-date World Health Organization's statistics demonstration, in married couple, unwillingly the average originating rate of property barrenness is 7%--46%, and is the trend increasing year by year.In China, along with the raising of industrialization degree, the people's work that increases the weight of year by year of atmosphere (haze) artificial environment pollution increases continuous increase negative, stress day by day, infertile patients is inevitable progressively to be increased, therefore, how can the infertile cause of disease be made rationally, to diagnose accurately, select the suitable pregnant means that help to there is great realistic meaning.
In andrology's semen analysis clinical examination, conventionally use at present " CASA " automatic detection analysis system, the problem existing for the general male sex can also be dealt with, for clinical the detection means that provides stronger of applying, for the analyzing and testing of seminal fluid provide accurately, science, method fast, particularly provide accurate data and detailed statistical description for sperm dynamic characteristic measuring, this causes attention and the favorable comment of the height of relevant expert, professor and the department head of government concerned thereof, medical workers.
But along with medical science and technology development, clinical requirement improve constantly, find through scholar's research both domestic and external, male sterility patient's " DNA fragment with spermatozoon meeting showed increased ", the percentage of head rice of DNA fragment with spermatozoon is to judge the effective foundation of sperm quality.DNA fragment with spermatozoon percentage of head rice not only can have influence on the fertility of sperm, even and if can be conceived, the formation of after fertilization protokaryon, also may cause miscarriage, offspring's congenital abnormality or suffer from some genetic disease.So just meet " CASA " the gained all technical that detects and be can not judge completely a male sex can pregnancy, can aristogenesis? or in-problem.The sperm quantity that detects due to the method using at present, concentration, vigor etc., each parameter fluctuation scope is large, and stability is not high, and then can not form unified standard, and then can't make prediction accurately and effectively to sperm quality and pregnancy outcome.And DNA fragment with spermatozoon rate helps pregnant patient greatly to affect and help one of pregnant successful unfavorable factor on intrauterine insemination (IUI).Therefore, the integrality of clinical examination sperm DNA has great realistic price to the assessment of infertile patients sperm quality.
Utility model content
The purpose of this utility model is the deficiency in order to overcome existed system function, design the many merits of a kind of ultra micro fluorescence sperm morphology and detect analytic system, for clinical provide a set of with better function, use more convenient system, and there is simple in structure, the advantage such as integrated level is high, volume is little, function is strong, processing ease.
A kind of ultra micro, the fluorescence sperm morphology multifunctional examining cls analysis system of the utility model design, is characterized in that, this system comprises main body, microcomputerized controller and the computing machine of system; This main body comprises the high power video camera being made up of camera and optical amplifier zoom camera lens, by the two-dimensional adjustment mechanism of object lens, eyepiece, movable working platform, sperm count pond, worktable and the optical microscope that pedestal forms, the fluorescent apparatus being formed by fluorescence lamp box, fluorescence spectrophotometer device, fluorescence power supply; The annexation of each parts is: high power video camera is arranged on the upper end of optical microscope by mechanical fastener, and the eyepiece of camera (CCD), optical amplifier zoom camera lens and optical microscope in high power video camera is coaxial; The optical splitter of described fluorescent apparatus is arranged in a framework, and this framework is fixed between the microscopical eyepiece and object lens of optical microscope; Level one side of this framework is connected with the connecting hole of fluorescence lamp box by mechanical fastener, and optical splitter is divided into the fluorescence receiving the blue streak illuminating vertically downward on worktable; The casing of this fluorescent lamp power supply is arranged on the pedestal of optical microscope main body, and the power supply of fluorescent light is connected with the fluorescence power supply of fluorescence lamp box by electric wire; Described microcomputerized controller, computing machine are connected with microscope body by circuit respectively.
The utility model system also comprises a stay-warm case with heating plate, and described main body is arranged in this stay-warm case, makes to detect to guarantee to work in the environment of 37 DEG C of left and right.
Also comprise a sleeve, the camera of described high power video camera and optical amplifier zoom camera lens are arranged in sleeve.
In described computing machine, store the collection of illustrative plates of known multiple different sperm concentrations, for simulating sperm template, improve the sperm concentration precision of test sample.
The utlity model has following characteristics and effect:
Native system is scientifically integrated optical microscope (Olypus-Bx41 or Bx51) and photoelectric amplification system, realizes stepless Free-scaling and amplifies 160 times of-30,000 times of adjustable Quality Controls, Chlamydia and mycoplasma that sperm tail is accompanying can clearly be detected.
Native system has solved initial trace demonstration of thermostat, density indeterminacy and quality control, sperm survival rate, detailed data list, sperm sequence number layout, sperm motility etc.In the time carrying out sperm detection, only detect sperm, all impurity are excluded, and have so just greatly improved the accuracy rate of sperm concentration.
The fluorescent apparatus of native system is cold light source system, and the cell (spermatoblast) exciting at fluorescence is to be beautiful complete form.
The utility model for clinical provide a set of with better function, use more convenient system, and there is simple in structure, the advantage such as integrated level is high, volume is little, function is strong, processing ease.
Brief description of the drawings
Fig. 1 is composition schematic diagram of the present utility model.
Embodiment
A kind of ultra micro of the utility model design, fluorescence sperm morphology multifunctional examining cls analysis system by reference to the accompanying drawings and embodiment be described in detail as follows:
System of the present utility model forms as shown in Figure 1, comprise the high power video camera 1 mainly being formed by camera and optical amplifier zoom camera lens, by the two-dimensional adjustment mechanism of object lens, eyepiece, movable working platform, sperm count pond, worktable and the optical microscope 2 that pedestal forms, the fluorescent apparatus being formed by fluorescence lamp box 4, fluorescence spectrophotometer device 3, fluorescence power supply 5, and microcomputerized controller 6 and computing machine (not shown); The annexation of each parts is: high power video camera 1 is arranged on the upper end of optical microscope 2 by mechanical fastener, and the eyepiece of camera (CCD), optical amplifier zoom camera lens and optical microscope 2 in high power video camera 1 is coaxial; The optical splitter 3 of described fluorescent apparatus is arranged in a framework, and this framework is fixed between the microscopical eyepiece and object lens of optical microscope 2; Level one side of this framework is connected with the connecting hole of fluorescence lamp box 4 by mechanical fastener, and optical splitter 3 is divided into the fluorescence receiving the blue streak illuminating vertically downward on worktable; The casing of this fluorescent lamp power supply 5 is arranged on the pedestal of optical microscope main body, and the power supply of fluorescent light is connected with the fluorescence power supply of fluorescence lamp box by electric wire.The main body of above-mentioned each parts construction system; Described microcomputerized controller, computing machine are connected with microscope body by circuit respectively.
The utility model system also comprises a stay-warm case with heating plate, and described main body is arranged in this stay-warm case.
Also comprise a sleeve, the camera of described high power video camera and optical amplifier zoom camera lens are arranged in sleeve.
In described computing machine, store the collection of illustrative plates of known multiple different sperm concentrations, for simulating sperm template, improve the precision of the sperm concentration of test sample.
Function and the embodiment of the each parts of native system are respectively described below:
High power video camera: camera adopts Hitachi-D20BP, for capturing the use of video image and signal.After adding D20BP device, can make system amplify 30,000 times.
Optical amplifier zoom camera lens adopts β multiplying power instrument (adopting existing device), so that control system changes enlarging function.
Optical microscope: adopt Olypus-Bx41 or Bx51 to amplify 1000 to 2000 times.Object lens are enlarged into: 10X, 20X, 40X, 100X; Eyepiece: be enlarged into 10X, 20X.Be responsible for video image collection.
Fluorescent apparatus: fluorescent light adopts power 100W-200W mercury lamp, for sending detection fluorescence.
Fluorescence spectrophotometer device: adopt stupefied mirror color separator, can decomposite the orthogonal blue light of direction and green glow.
Fluorescence is NFP-1(Ac-100-240V for power acquisition) be fluorescent light power supply.
(X, Y, Z) adopts three-dimensional motion controller (being conventional ripening product), be divided on microscope stage three micro-step motors 8,9 and 7(as shown in Figure 1 for controlling) do the accurate motion of three directions, its motion size is carried out precision by it to three-dimensional (X, Y, Z) completely and is controlled, the Dynamic Signal of three stepper motors, links controller by signal wire and automatically controls.
Computing machine: adopt the high performance brand name computer of asking magnificent Tongfang to be produced: P-IV-500 are overhead controls of the utility model image, signal processing, calculating, report output etc.Interior installing sperm, fluorescence sperm, three capture cards of DNA fragment with spermatozoon (capture card is known technology).
Native system can be applied following aspect:
Native system will differ optical microscope and (adopt Olypus-Bx41 or Bx51, eyepiece amplifies 10X doubly and 20X times, object lens amplify 10X, 20X, 40X, 100X and are amplified to 2000 times) and photoelectric amplification system scientifically integrate, can by end slightly blood carry out the assessment of human-body sub-health, and provide dietotherapy guidance program, carry out infantile tumour examination; Also can carry out live body detection to the sick body in various body fluid, secretion, microorganism, Chlamydia and mycoplasma that sperm tail is accompanying can clearly be detected.
Native system is according to the having or not of halation, DNA fragment integrated degree that size judges sperm.DNA fragment with spermatozoon is relevant to sperm morphology, vigor and motility rate.For the rate of fertilization of prediction standard I VF provides side information, even may predict natural pregnancy rate.DNA fragment with spermatozoon chromatin Structure analyze (SCSA) can predictor in, failed generation in vitro fertilization.
Claims (3)
1. ultra micro, a fluorescence sperm morphology multifunctional examining cls analysis system, is characterized in that, this system comprises main body, microcomputerized controller and the computing machine of system; This main body comprises the high power video camera being made up of camera and optical amplifier zoom camera lens, by the two-dimensional adjustment mechanism of object lens, eyepiece, movable working platform, sperm count pond, worktable and the optical microscope that pedestal forms, the fluorescent apparatus being formed by fluorescence lamp box, fluorescence spectrophotometer device, fluorescence power supply; The annexation of each parts is: high power video camera is arranged on the upper end of optical microscope by mechanical fastener, and the eyepiece of camera, optical amplifier zoom camera lens and optical microscope in high power video camera is coaxial; The optical splitter of described fluorescent apparatus is arranged in a framework, and this framework is fixed between the microscopical eyepiece and object lens of optical microscope; Level one side of this framework is connected with the connecting hole of fluorescence lamp box by mechanical fastener, and optical splitter is divided into the fluorescence receiving the blue streak illuminating vertically downward on worktable; The casing of this fluorescent lamp power supply is arranged on the pedestal of optical microscope main body, and the power supply of fluorescent light is connected with the fluorescence power supply of fluorescence lamp box by electric wire; Described microcomputerized controller, computing machine are connected with microscope body by circuit respectively.
2. system as claimed in claim 1, is characterized in that, this system also comprises a stay-warm case with heating plate, and described main body is arranged in this stay-warm case.
3. system as claimed in claim 1, is characterized in that, this system also comprises a sleeve, and the camera of described high power video camera and optical amplifier zoom camera lens are arranged in sleeve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420185062.XU CN203894149U (en) | 2014-04-16 | 2014-04-16 | Supermicron fluorescence sperm morphology multifunctional detection and analysis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420185062.XU CN203894149U (en) | 2014-04-16 | 2014-04-16 | Supermicron fluorescence sperm morphology multifunctional detection and analysis system |
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| Publication Number | Publication Date |
|---|---|
| CN203894149U true CN203894149U (en) | 2014-10-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420185062.XU Expired - Fee Related CN203894149U (en) | 2014-04-16 | 2014-04-16 | Supermicron fluorescence sperm morphology multifunctional detection and analysis system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104698158A (en) * | 2015-02-25 | 2015-06-10 | 刘敬松 | Device for detecting sperm activity |
| CN109064469A (en) * | 2018-10-31 | 2018-12-21 | 北京新网视信传媒科技有限公司 | Sperm quality detector and sperm quality detection system |
| CN111500530A (en) * | 2020-04-30 | 2020-08-07 | 延安大学 | Universal animal sperm sorting method and X sperm quality control method |
-
2014
- 2014-04-16 CN CN201420185062.XU patent/CN203894149U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104698158A (en) * | 2015-02-25 | 2015-06-10 | 刘敬松 | Device for detecting sperm activity |
| CN109064469A (en) * | 2018-10-31 | 2018-12-21 | 北京新网视信传媒科技有限公司 | Sperm quality detector and sperm quality detection system |
| CN109064469B (en) * | 2018-10-31 | 2023-08-15 | 北京新网视信传媒科技有限公司 | Sperm quality detector and sperm quality detection system |
| CN111500530A (en) * | 2020-04-30 | 2020-08-07 | 延安大学 | Universal animal sperm sorting method and X sperm quality control method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141022 |