CN106367330B - A kind of microbial morphology shooting identification apparatus and method - Google Patents
A kind of microbial morphology shooting identification apparatus and method Download PDFInfo
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Abstract
The invention discloses a kind of microbial morphology shooting identification apparatus and methods, belong to microorganism detection field, the device includes cabinet (16), lighting source (10), uniform light board (11), imaging sensor (12), image pick-up card (13), record identification systems (14), uniform light board (11) are equipped on the inside of cabinet (16), are equipped with lighting source (10) between uniform light board (11) and cabinet (16)；Imaging sensor (12) is located at the top surface of cabinet (16), and imaging sensor (12) is connect with the output end of image pick-up card (13), image pick-up card (13) with record identification systems (14).The microbial morphology picture of acquisition is carried out feature extraction and pattern-recognition by being acquired to microbial morphology, with computer vision technique by this method, carries out microbial identification in conjunction with biochemical, mass spectrography.Apparatus of the present invention structure is simple and convenient to operate, identifies quickly, accurately.
Description
Technical field
The present invention relates to microorganism detection fields, and in particular to a kind of microbial morphology shooting identification apparatus and method.
Background technique
The identification of microorganism plays key player in clinic, is the foundation of related disease diagnosing and treating.In recent years,
There are many new identification technologies and method, such as full-automatic biochemical reacts identification systems, Mass Spectrometric Identification system.Although above-mentioned
Advanced technology has been developed that, but since its equipment price is expensive, complicated for operation, so that traditional microbial morphology
It learns identification method still to occupy an important position, is the basis of microorganism classification and identification, it is indispensable.
Microbial morphology identification mainly includes culture dish sample and microscope flowering structure, and culture dish sample is mainly used for seeing
Examine the features such as size, appearance, edge configuration, color, gloss, the haemolysis situation of bacterium colony；The micro- life of microscope flowering structure main detection
The number and form of size, shape, arrangement, the dyeability of object individual, mycelia and spore, whether there is or not brood cell, pod membrane and flagellum etc. are special
Different structure, and further combined with biochemical, mass spectrum property, identification classification is carried out to microorganism with this.
In medical photography, microbial morphology is the important object of shooting, and the whether true of feature instantiation, protrusion are
The standard of measurement shooting quality, and shooting condition (color of such as light source, light angle, luminous intensity, shooting angle, image sensing
Device etc.) directly influence the quality of microbial morphology picture.
Chinese invention patent application 104561229A discloses the culture and shape of a kind of nanometer bacteria on April 29th, 2015
State identification method；2010《Jilin University》It discloses and " based on biotechnology and calculates in the Ph.D. Dissertation of the fourth skin of bamboo
The food microorganisms of machine vision quickly detect research ".But it not yet retrieves in the prior art and microbial culture dish form is carried out
The relevant device of shooting and identification.
Summary of the invention
The object of the present invention is to provide a kind of microbial morphology shooting identification apparatus and method, the apparatus structure is simple,
Easy to operate, identification is quickly, accurately.
To achieve the above object, technical scheme is as follows：
A kind of microbial morphology shooting identification apparatus, which includes cabinet 16, lighting source 10, uniform light board 11, image
Sensor 12, image pick-up card 13, record identification systems 14, wherein 16 side of cabinet is equipped with opening, sets on the inside of cabinet 16
There is uniform light board 11, lighting source 10 is equipped between uniform light board 11 and cabinet 16；The top surface of cabinet 16 is equipped with through-hole, imaging sensor
12 are located at the top surface of cabinet 16, and corresponding with through-hole, and imaging sensor 12 is connect with the input terminal of image pick-up card 13, image
The output end of capture card 13 is connect with the input terminal of record identification systems 14.
The brightness of the lighting source 10 and Color tunable；The shooting angle of described image sensor 12 is adjustable.
The cross section of the uniform light board 11 is arc-shaped or rectangle.
The lighting source 10 is LED light, incandescent lamp, halogen lamp or fluorescent lamp.
The uniform light board 11 is organic glass, polyformaldehyde or nylon.
Described image sensor 12 and image pick-up card 13, image pick-up card 13 and the connection type for recording identification systems 14
For wired connection or wireless connection.
A kind of microbial morphology shooting identification method using the device, includes the following steps：
1) microbial culture dish 15 for filling microbe colony is placed in the bottom of cabinet 1, opens lighting source 1, it will
To desired value, the light that lighting source 1 issues is irradiated to micro- after the reflection and refraction of equal tabula rasa 11 for brightness and color adaptation
On bacterium colony in biological culture dish 15；
2) imaging sensor 12 is opened, the colonial morphology in microbial culture dish 15 is acquired；
3) collected colonial morphology is transmitted to image pick-up card 13, and be further conveyed in record identification systems 14
It is recorded and is identified.
The strong and weak control that lighting source 10 shines is carried out by the way of adjusting electric current or adjusting voltage.
In step 3), the microbial morphology picture of acquisition is subjected to feature extraction with computer vision technique and mode is known
Not.
Feature extraction and pattern-recognition include the following steps：
The separation of bacterium colony and background, i.e. carrying out image threshold segmentation are carried out according to gray feature；Followed by Edge extraction
The feature extraction of colonial morphology is carried out with gray scale morphology algorithm；Finally using neural network, support vector machine algorithm to current
Feature classified and retrieved, carry out the differentiation of strain.
The beneficial effects of the present invention are：
Microbial morphology shooting identification apparatus of the invention and method pass through the lighting source of adjustable brightness and color,
Under the reflection and refraction action of uniform light board, by direct irradiation and back lighting, the image sensing of adjustable shooting angle is utilized
Device, wired or wireless connection image pick-up card shooting bacterium colony size, appearance, edge configuration, color, gloss, haemolysis situation
Etc. features, and features described above is identified using record identification systems, is realized in combination with identification of means such as mass spectrum, biochemistry fast
Speed, precise Identification.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that microbial morphology of the invention shoots identification apparatus.
Fig. 2 is the partial schematic diagram that microbial morphology of the invention shoots identification apparatus.
Appended drawing reference
10 lighting sources
11 uniform light boards
12 imaging sensors
13 image pick-up cards
14 record identification systems
15 microbial culture dishs
16 cabinets
Specific embodiment
With reference to the accompanying drawing, specific embodiments of the present invention will be described in further detail.
Microbial morphology shooting identification apparatus of the invention and method use meter by being acquired to microbial morphology
The microbial morphology picture of acquisition is carried out feature extraction and pattern-recognition by calculation machine vision technique, is carried out in conjunction with biochemical, mass spectrography
The Rapid identification of microorganism.
As shown in Figs. 1-2, microbial morphology of the invention shooting identification apparatus includes cabinet 16, for illuminating microorganism shape
The lighting source 10 of the brightness of state and Color tunable, the uniform light board 11, micro- for shooting for uniform protection lighting source 10
The adjustable imaging sensor 12 of the shooting angle of biomorph and acquisition transmit the image pick-up card 13 of image data, for recording
With the record identification systems 14 with computer vision technique of identification microbial morphology.
16 side of cabinet is equipped with opening, for picking and placing microbial culture dish 15, is equipped with uniform light board 11, even light on the inside of cabinet 16
Lighting source 10 is equipped between plate 11 and cabinet 16；The top surface of cabinet 16 is equipped with through-hole, and imaging sensor 12 is located at cabinet 16
Top surface, and it is corresponding with through-hole, described image sensor 12 can carry out global and details by optics and number amplification and shoot；
Imaging sensor 12 is connect with the input terminal of image pick-up card 13, output end and the record identification systems 14 of image pick-up card 13
Input terminal connection.
The cross section of the uniform light board 11 is arc-shaped or rectangle.
The lighting source 10 is preferably LED light, incandescent lamp, halogen lamp or fluorescent lamp, and lighting source 10 is capable of providing directly
Irradiation and back lighting are connect, distribution angle is arc-shaped or rectangle, is able to carry out brightness and color is adapted to different cultures
The shooting of different colonial morphologies on base.
The uniform light board 11 is preferably organic glass, polyformaldehyde or nylon, and uniform light board 11 can be by lighting source 10
The refraction and reflection to emit beam makes maintaining uniform illumination.
Described image sensor 12 and image pick-up card 13, image pick-up card 13 and the connection type for recording identification systems 14
For wired connection or wireless connection.For example, usb protocol connection can be used in wired connection mode, sent out by record identification systems 14
The instruction for obtaining image is sent, imaging sensor 12 is exposed and is imaged, and modulus is carried out under the action of image pick-up card 13 and is turned
It changes, and digital information is sent to record identification systems 14 by USB connecting line, complete Image Acquisition.Wireless mode can then lead to
WIFI agreement is crossed, specific collection process is consistent with above-mentioned wired connection mode.
Microbial morphology shooting identification method of the invention includes the following steps：
1) microbial culture dish 15 for filling microbe colony is placed in the bottom of cabinet 1, opens lighting source 1, it will
To desired value, the light that lighting source 1 issues is irradiated to micro- after the reflection and refraction of equal tabula rasa 11 for brightness and color adaptation
On bacterium colony in biological culture dish 15；
It is carried out carrying out the hair of lighting source 10 based on the mode for adjusting electric current or adjusting voltage according to the characteristics of lighting source 10
The strong and weak control of light.For example, LED light source is driven using current constant mode, and current regulation, Jin Ergai are carried out by circuits such as amplifiers
Become luminous intensity；Incandescent lamp makes to be added to day by the SCR light regulation circuit with rectifier bridge by changing the angle of flow of thyristor
The power frequency ac voltage at light lamp tube both ends changes, and then changes luminous intensity.
2) imaging sensor 12 is opened, to colonial morphology (such as size, appearance, the edge shape in microbial culture dish 15
The features such as state, color, gloss, haemolysis situation) it is acquired；
3) collected colonial morphology is transmitted to image pick-up card 13, and be further conveyed in record identification systems 14
It is recorded and is identified, the separation of bacterium colony and background, i.e. carrying out image threshold segmentation are carried out according to gray feature；Followed by image side
Edge extracts and gray scale morphology scheduling algorithm carries out the feature extraction of colonial morphology；Finally utilize neural network, support vector machine etc.
Algorithm is classified and is retrieved to current feature, and the differentiation of strain is carried out.
Embodiment 1
Embodiment 1 is by taking the shooting and identification of escherichia coli (Escherichia coli) as an example.
Escherichia coli belongs to Escherichia, is aerobic or facultative anaerobic bacteria, and 35 DEG C of incubations 24 are small on blood agar plate
When, the microbial culture dish 15 for filling escherichia coli is placed in microbial morphology shooting identification apparatus of the invention, is led to
Cross arc-shaped setting brightness 80%, color is set as lily lighting source 10, organic glass uniform light board 11 it is anti-
It penetrates under refraction action, direct irradiation and back lighting by lighting source 10 are 90 degree of figure using shooting angle is adjusted
As sensor 12, by WIFI be wirelessly connected image pick-up card 13 record the size of bacterium colony in microbial culture dish 15, appearance,
The features such as edge configuration, color, gloss, haemolysis situation, and features described above is recorded and known by recording identification systems 14
Not, according to the colony characteristics of escherichia coli, it is whole for 2~3mm, slightly convex, round, wet, canescence, edge that diameter can be formed
Together, opaque colony, while in combination with mass spectrum and biochemical identification, determine that this bacterium for escherichia coli, completes shooting and identification.
The above is only a specific embodiment of the present invention, but the design concept of the present invention is not limited to this, all to utilize this
Design makes a non-material change to the present invention, and should all belong to behavior that violates the scope of protection of the present invention.
Claims (9)
1. a kind of microbial morphology shoots identification apparatus, it is characterised in that：The device include cabinet (16), lighting source (10),
Uniform light board (11), imaging sensor (12), image pick-up card (13), record identification systems (14), wherein cabinet (16) side
Face is equipped with opening, is equipped with uniform light board (11) on the inside of cabinet (16), lighting source is equipped between uniform light board (11) and cabinet (16)
(10)；The top surface of cabinet (16) is equipped with through-hole, and imaging sensor (12) is located at the top surface of cabinet (16), and corresponding with through-hole,
Imaging sensor (12) is connect with the input terminal of image pick-up card (13), the output end of image pick-up card (13) and record identification system
The input terminal connection of system (14)；The brightness of the lighting source (10) and Color tunable；The shooting of described image sensor (12)
Adjustable angle.
2. microbial morphology according to claim 1 shoots identification apparatus, it is characterised in that：The cross of the uniform light board (11)
Section is arc-shaped or rectangle.
3. microbial morphology according to claim 1 shoots identification apparatus, it is characterised in that：The lighting source (10) is
LED light, incandescent lamp, halogen lamp or fluorescent lamp.
4. microbial morphology according to claim 1 shoots identification apparatus, it is characterised in that：The uniform light board (11) is to have
Machine glass, polyformaldehyde or nylon.
5. microbial morphology according to claim 1 shoots identification apparatus, it is characterised in that：Described image sensor (12)
It is wired connection with the connection type of image pick-up card (13), image pick-up card (13) and record identification systems (14) or wirelessly connects
It connects.
6. a kind of microbial morphology using device according to claim 1-5 shoots identification method, feature
It is：
Include the following steps：
1) microbial culture dish (15) for filling microbe colony is placed in the bottom of cabinet (1), is opened lighting source (1),
By brightness and color adaptation to desired value, the light that lighting source (1) issues shines after the reflection and refraction of uniform light board (11)
It is mapped on the bacterium colony in microbial culture dish (15)；
2) imaging sensor (12) are opened, the colonial morphology in microbial culture dish (15) is acquired；
3) collected colonial morphology is transmitted to image pick-up card (13), and be further conveyed in record identification systems (14)
It is recorded and is identified.
7. microbial morphology according to claim 6 shoots identification method, it is characterised in that：Using adjusting electric current or adjusting
The mode of voltage carries out the strong and weak control that lighting source (10) shine.
8. microbial morphology according to claim 6 shoots identification method, it is characterised in that：In step 3), with calculating
The microbial morphology picture of acquisition is carried out feature extraction and pattern-recognition by machine vision technique.
9. microbial morphology according to claim 8 shoots identification method, it is characterised in that：Feature extraction and pattern-recognition
Include the following steps：
The separation of bacterium colony and background, i.e. carrying out image threshold segmentation are carried out according to gray feature；Followed by Edge extraction and ash
Spend the feature extraction that Morphology Algorithm carries out colonial morphology；Finally using neural network, support vector machine algorithm to current spy
Sign is classified and is retrieved, and the differentiation of strain is carried out.
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| CN201611032836.5A CN106367330B (en) | 2016-11-19 | 2016-11-19 | A kind of microbial morphology shooting identification apparatus and method |
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| CN106367330B true CN106367330B (en) | 2018-11-27 |
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| CN107680129B (en) * | 2017-09-11 | 2020-10-20 | 浙江大学 | Portable bacterial colony automatic counting method based on smart phone |
| CN109852663A (en) * | 2019-04-02 | 2019-06-07 | 天津科技大学 | A kind of method and system based on machine vision high throughput screening of microorganisms |
| CN110675386B (en) * | 2019-09-26 | 2023-08-18 | 北京大学第一医院 | Detection system for group B streptococcus |
| CN112798593A (en) * | 2020-12-28 | 2021-05-14 | 南京威派视半导体技术有限公司 | Microscopic observation device and method for liquid-based sample |
| CN113025483A (en) * | 2021-03-24 | 2021-06-25 | 四川若斌生物科技有限责任公司 | Acquisition and analysis system and analysis method of microorganism culture and analysis cabinet |
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| CN2554658Y (en) * | 2002-05-16 | 2003-06-04 | 唯特科技股份有限公司 | Symmetric light-source lamp box of uniform lighting |
| CN201804346U (en) * | 2010-06-03 | 2011-04-20 | 合肥瑞韬电子科技有限公司 | Full automatic colony counter |
| JP6595156B2 (en) * | 2014-03-04 | 2019-10-23 | 富士フイルム株式会社 | Cell image acquisition apparatus and method, and program |
| CN104561229A (en) * | 2015-01-19 | 2015-04-29 | 中国人民解放军第三七一医院 | Nanobacteria culture and morphological identification method |
| CN104749180B (en) * | 2015-03-25 | 2017-09-01 | 北京浩辰星月科技有限公司 | Machine vision identification and analysis and susceptibility identification systems |
| CN105115970A (en) * | 2015-06-30 | 2015-12-02 | 厦门质谱仪器仪表有限公司 | Phenotypic identification method and mass spectrometry method comprehensive microorganism identification system and phenotypic identification method and mass spectrometry method comprehensive microorganism identification method |
| CN105954281B (en) * | 2016-04-21 | 2018-07-27 | 南京农业大学 | A kind of paddy goes mouldy the method for fungus colony non-damage drive |
| CN206204318U (en) * | 2016-11-19 | 2017-05-31 | 厦门大学 | A kind of microbial morphology shoots identification apparatus |
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Inventor after: Feng Bin Inventor after: He Jian Inventor before: He Jian Inventor before: Feng Bin |