CN106367330A - Shooting identification device and method for morphology of microorganisms - Google Patents
Shooting identification device and method for morphology of microorganisms Download PDFInfo
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- CN106367330A CN106367330A CN201611032836.5A CN201611032836A CN106367330A CN 106367330 A CN106367330 A CN 106367330A CN 201611032836 A CN201611032836 A CN 201611032836A CN 106367330 A CN106367330 A CN 106367330A
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- morphology
- microbial
- shoot
- lighting source
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Abstract
The invention discloses a shooting identification device and method for morphology of microorganisms, and belongs to the field of microbiological detection. The device comprises a box body (16), a lighting source (10), a dodging plate (11), an image sensor (12), an image acquisition card (13) and a record identification system (14), wherein the dodging plate (11) is arranged on the inner side of the box body (16), and the lighting source (10) is arranged between the dodging plate (11) and the box body (16); the image sensor (12) is located on the top face of the box body (16), the image sensor (12) is connected with the image acquisition card (13), and the output end of the image acquisition card (13) is connected with the record identification system (14). According to the method, the morphology of the microorganisms is collected, feature extraction and pattern recognition are performed on the obtained microorganism morphological images by means of a computer vision technology, and the microorganisms are identified combined with biochemistry and mass spectrometry. The device is simple in structure, convenient to operate, and fast and accurate in identification.
Description
Technical field
The present invention relates to microorganism detection field is and in particular to a kind of microbial morphology shoots identification apparatus and method.
Background technology
The identification of microorganism plays key player in clinic, is the foundation of relevant disease diagnosis and treatment.In recent years,
Occur in that many new identification technologies and method, such as full-automatic biochemical reaction identification systems, Mass Spectrometric Identification system etc..Although it is above-mentioned
Advanced technology has been developed that, but because its equipment price is expensive, complex operation is so that traditional microbial morphology
Learn authentication method and still occupy critical role, be microorganism classification and the basis of identification, indispensable.
Microbial morphology identification is main to include culture dish specimen and microscopic structures, and culture dish specimen is mainly used in seeing
Examine the features such as the size of bacterium colony, outward appearance, edge configuration, color, gloss, haemolysis situation；The micro- life of microscopic structures main detection
The individual size of thing, shape, arrangement, dyeability, the number of mycelia and spore and form, have or not the spies such as brood cell, pod membrane and flagellum
Different structure, and further combined with biochemical, mass spectrum property, identification classification is carried out with this to microorganism.
In medical photography, microbial morphology be shoot important object, its feature instantiation whether true, prominent, be
Weigh the standard of shooting quality, and shooting condition is (as the color of light source, light angle, light intensity, shooting angle, image sensing
Device etc.) directly influence the quality of microbial morphology picture.
Chinese invention patent application 104561229a discloses a kind of culture of nanometer bacteria and shape on April 29th, 2015
State authentication method；Disclose " based on biotechnology and calculating in the Ph.D. Dissertation of " Jilin University " fourth skin of bamboo of 2010
The food microorganisms quick detection research of machine vision ".But not yet retrieve in prior art and microbial culture dish form is carried out
The relevant device shooting and identifying.
Content of the invention
It is an object of the invention to, provide a kind of microbial morphology to shoot identification apparatus and method, this apparatus structure is simple,
Easy to operate, identification is quick, accurately.
For achieving the above object, technical scheme is as follows:
A kind of microbial morphology shoots identification apparatus, and this device includes casing 16, lighting source 10, even tabula rasa 11, image
Sensor 12, image pick-up card 13, record identification systems 14, wherein, described casing 16 side is provided with opening, sets inside casing 16
There is even tabula rasa 11, between even tabula rasa 11 and casing 16, be provided with lighting source 10；The top surface of casing 16 is provided with through hole, imageing sensor
12 top surfaces being located at casing 16, and corresponding with through hole, imageing sensor 12 is connected with the input of image pick-up card 13, image
The outfan of capture card 13 is connected with the input of record identification systems 14.
The brightness of described lighting source 10 and Color tunable；The shooting angle of described image sensor 12 is adjustable.
The cross section of described even tabula rasa 11 is circular arc or rectangle.
Described lighting source 10 is LED lamp, electric filament lamp, Halogen light or fluorescent lamp.
Described even tabula rasa 11 is lucite, polyformaldehyde or nylon.
Described image sensor 12 and image pick-up card 13, image pick-up card 13 and the connected mode recording identification systems 14
For wired connection or wireless connection.
A kind of microbial morphology using described device shoots authentication method, comprises the steps:
1) microbial culture dish 15 filling microbe colony is positioned over the bottom of casing 1, opens lighting source 1, will
, to desired value, the light that lighting source 1 sends is irradiated to micro- after the reflection and refraction of equal tabula rasa 11 for brightness and color adaptation
On bacterium colony in biological culture dish 15；
2) open imageing sensor 12, the colonial morphology in microbial culture dish 15 is acquired；
3) colonial morphology collecting is sent to image pick-up card 13, and is further conveyed to record in identification systems 14
Recorded and identified.
Controlled using adjusting electric current or being illuminated the luminous power of light source 10 by the way of adjusting voltage.
Step 3) in, with computer vision technique, the microbial morphology picture of acquisition is carried out feature extraction and pattern is known
Not.
Feature extraction and pattern recognition comprise the steps:
Carry out the separation of bacterium colony and background according to gray feature, i.e. carrying out image threshold segmentation；Followed by Edge extraction
Carry out the feature extraction of colonial morphology with gray scale morphology algorithm；Finally utilize neutral net, algorithm of support vector machine to current
Feature classified and retrieved, carry out the differentiation of strain.
The beneficial effects of the present invention is:
The microbial morphology of the present invention shoots identification apparatus and method passes through the lighting source of adjustable brightness and color,
Under the reflection and refraction action of even tabula rasa, by direct irradiation and back lighting, using the image sensing of adjustable shooting angle
Device, the size of image pick-up card shooting bacterium colony of wired or wireless connection, outward appearance, edge configuration, color, gloss, haemolysis situation
Etc. feature, and using record identification systems, features described above is identified, realizes in combination with identification of means such as mass spectrum, biochemistry fast
Speed, precise Identification.
Brief description
Fig. 1 is the structural representation of the microbial morphology shooting identification apparatus of the present invention.
Fig. 2 is the partial schematic diagram of the microbial morphology shooting identification apparatus of the present invention.
Reference
10 lighting sources
11 even tabula rasas
12 imageing sensors
13 image pick-up cards
14 record identification systems
15 microbial culture dishs
16 casings
Specific embodiment
Below in conjunction with the accompanying drawings, the specific embodiment of the present invention is described in further detail.
The microbial morphology of the present invention shoots identification apparatus and method by being acquired to microbial morphology, and with meter
The microbial morphology picture of acquisition is carried out feature extraction and pattern recognition by calculation machine vision technique, carries out in conjunction with biochemical, mass spectrography
The Rapid identification of microorganism.
As shown in Figure 1-2, the microbial morphology of the present invention shoots identification apparatus and includes casing 16, is used for illuminating microorganism shape
The lighting source 10 of the brightness of state and Color tunable, the even tabula rasa 11 for all even protection lighting source 10, be used for shooting micro-
The adjustable imageing sensor of biomophic shooting angle 12 and obtain the image pick-up card 13 of transmission view data, be used for recording
Record identification systems 14 with the utilization computer vision technique of identification microbial morphology.
Casing 16 side is provided with opening, is used for picking and placeing microbial culture dish 15, is provided with even tabula rasa 11, even light inside casing 16
It is provided with lighting source 10 between plate 11 and casing 16；The top surface of casing 16 is provided with through hole, and imageing sensor 12 is located at casing 16
Top surface, and corresponding with through hole, described image sensor 12 can carry out the overall situation by optics and numeral amplification and details shoots；
Imageing sensor 12 is connected with the input of image pick-up card 13, the outfan of image pick-up card 13 and record identification systems 14
Input connects.
The cross section of described even tabula rasa 11 is circular arc or rectangle.
Described lighting source 10 is preferably LED lamp, electric filament lamp, Halogen light or fluorescent lamp, and lighting source 10 can provide directly
Connect irradiation and back lighting, distribution angle is in circular arc or rectangle, brightness can be carried out and color is adapted to different cultures
The shooting of different colonial morphologies on base.
Described even tabula rasa 11 is preferably lucite, polyformaldehyde or nylon, and even tabula rasa 11 can be by lighting source 10
The refraction emitting beam and reflection make maintaining uniform illumination.
Described image sensor 12 and image pick-up card 13, image pick-up card 13 and the connected mode recording identification systems 14
For wired connection or wireless connection.For example, wired connection mode can be connected using usb agreement, by recording identification systems 14
Send the instruction obtaining image, imageing sensor 12 is exposed and is imaged, and carries out modulus and turn in the presence of image pick-up card 13
Change, and digital information is sent to record identification systems 14 by usb connecting line, complete image acquisition.Wireless mode then can lead to
Cross wifi agreement, concrete gatherer process is consistent with above-mentioned wired connection mode.
The microbial morphology of the present invention shoots authentication method and comprises the steps:
1) microbial culture dish 15 filling microbe colony is positioned over the bottom of casing 1, opens lighting source 1, will
, to desired value, the light that lighting source 1 sends is irradiated to micro- after the reflection and refraction of equal tabula rasa 11 for brightness and color adaptation
On bacterium colony in biological culture dish 15；
The mode that feature according to lighting source 10 is carried out based on adjusting electric current or regulation voltage is illuminated light source 10
The strong and weak control of light.For example, LED light source adopts current constant mode to drive, and carries out electric current regulation, Jin Ergai by circuit such as amplifiers
Become luminous intensity；Electric filament lamp, by the SCR light regulation circuit with rectifier bridge, by changing the angle of flow of IGCT, makes to be added to day
The power frequency ac voltage at light lamp tube two ends changes, and then changes luminous intensity.
2) open imageing sensor 12, to colonial morphology (such as size, outward appearance, edge shape in microbial culture dish 15
The features such as state, color, gloss, haemolysis situation) it is acquired；
3) colonial morphology collecting is sent to image pick-up card 13, and is further conveyed to record in identification systems 14
Recorded and identified, carried out the separation of bacterium colony and background according to gray feature, i.e. carrying out image threshold segmentation；Followed by image side
Edge extracts the feature extraction carrying out colonial morphology with gray scale morphology scheduling algorithm；Finally utilize neutral net, support vector machine etc.
Algorithm is classified to current feature and is retrieved, and carries out the differentiation of strain.
Embodiment 1
Embodiment 1 is taking the shooting of escherichia coli (escherichia coli) and identification as a example.
Escherichia coli belongs to Escherichia, is aerobic or facultative anaerobe, and on blood agar plate, 35 DEG C of incubations 24 are little
When, the microbial morphology that the microbial culture dish 15 filling escherichia coli is placed on the present invention shoots in identification apparatus, leads to
Cross the setting brightness 80% of circular arc, color is set to lily lighting source 10, lucite even tabula rasa 11 anti-
Penetrate with refraction action under, by direct irradiation and the back lighting of lighting source 10, using adjusting the figure that shooting angle is 90 degree
As sensor 12, by wifi wirelessly connect image pick-up card 13 record the size of the bacterium colony in microbial culture dish 15, outward appearance,
The features such as edge configuration, color, gloss, haemolysis situation, and by record identification systems 14, features described above is recorded and know
Not, the colony characteristicses according to escherichia coli, you can form a diameter of 2～3mm, slightly convex, circular, moistening, canescence, edge whole
Together, opaque colony, can judge this bacterium as escherichia coli in conjunction with mass spectrum and biochemical identification simultaneously, complete to shoot and identify.
Above are only the specific embodiment of the present invention, but the design concept of the present invention is not limited thereto, all utilize this
Design carries out the change of unsubstantiality to the present invention, all should belong to the behavior invading the scope of the present invention.
Claims (10)
1. a kind of microbial morphology shoot identification apparatus it is characterised in that:
This device include casing (16), lighting source (10), even tabula rasa (11), imageing sensor (12), image pick-up card (13),
Record identification systems (14), wherein,
Described casing (16) side is provided with opening, is provided with even tabula rasa (11), even tabula rasa (11) and casing (16) inside casing (16)
Between be provided with lighting source (10)；The top surface of casing (16) is provided with through hole, and imageing sensor (12) is located at the top surface of casing (16),
And corresponding with through hole, imageing sensor (12) is connected with the input of image pick-up card (13), image pick-up card (13) defeated
The input going out end with record identification systems (14) is connected.
2. microbial morphology according to claim 1 shoot identification apparatus it is characterised in that:
The brightness of described lighting source (10) and Color tunable；
The shooting angle of described image sensor (12) is adjustable.
3. microbial morphology according to claim 1 shoot identification apparatus it is characterised in that:
The cross section of described even tabula rasa (11) is circular arc or rectangle.
4. microbial morphology according to claim 1 shoot identification apparatus it is characterised in that:
Described lighting source (10) is LED lamp, electric filament lamp, Halogen light or fluorescent lamp.
5. microbial morphology according to claim 1 shoot identification apparatus it is characterised in that:
Described even tabula rasa (11) is lucite, polyformaldehyde or nylon.
6. microbial morphology according to claim 1 shoot identification apparatus it is characterised in that:
Described image sensor (12) and image pick-up card (13), image pick-up card (13) and the connection recording identification systems (14)
Mode is wired connection or wireless connection.
7. a kind of microbial morphology using the device according to any one of claim 1-6 shoots authentication method, its feature
It is:
Comprise the steps:
1) microbial culture dish (15) filling microbe colony is positioned over the bottom of casing (1), opens lighting source (1),
By brightness and color adaptation to desired value, the light that lighting source (1) sends shines after the reflection and refraction of equal tabula rasa (11)
It is mapped on the bacterium colony in microbial culture dish (15)；
2) open imageing sensor (12), the colonial morphology in microbial culture dish (15) is acquired；
3) colonial morphology collecting is sent to image pick-up card (13), and is further conveyed to record in identification systems (14)
Recorded and identified.
8. microbial morphology according to claim 7 shoot authentication method it is characterised in that:
Controlled using adjusting electric current or being illuminated the luminous power of light source (10) by the way of adjusting voltage.
9. microbial morphology according to claim 7 shoot authentication method it is characterised in that:
Step 3) in, with computer vision technique, the microbial morphology picture of acquisition is carried out feature extraction and pattern recognition.
10. microbial morphology according to claim 9 shoot authentication method it is characterised in that:
Feature extraction and pattern recognition comprise the steps:
Carry out the separation of bacterium colony and background according to gray feature, i.e. carrying out image threshold segmentation；Followed by Edge extraction and ash
Degree Morphology Algorithm carries out the feature extraction of colonial morphology；Finally utilize neutral net, algorithm of support vector machine to current spy
Levy and classified and retrieve, carry out the differentiation of strain.
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| CN107680129A (en) * | 2017-09-11 | 2018-02-09 | 浙江大学 | A kind of portable bacterium colony robot scaler and method based on smart mobile phone |
| CN109852663A (en) * | 2019-04-02 | 2019-06-07 | 天津科技大学 | A kind of method and system based on machine vision high throughput screening of microorganisms |
| CN110675386A (en) * | 2019-09-26 | 2020-01-10 | 北京大学第一医院 | Detection system of B-group streptococcus |
| CN112798593A (en) * | 2020-12-28 | 2021-05-14 | 南京威派视半导体技术有限公司 | Microscopic observation device and method for liquid-based sample |
| CN113025483A (en) * | 2021-03-24 | 2021-06-25 | 四川若斌生物科技有限责任公司 | Acquisition and analysis system and analysis method of microorganism culture and analysis cabinet |
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