CN206204318U - A kind of microbial morphology shoots identification apparatus - Google Patents
A kind of microbial morphology shoots identification apparatus Download PDFInfo
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- CN206204318U CN206204318U CN201621254188.3U CN201621254188U CN206204318U CN 206204318 U CN206204318 U CN 206204318U CN 201621254188 U CN201621254188 U CN 201621254188U CN 206204318 U CN206204318 U CN 206204318U
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- casing
- card
- image pick
- uniform light
- light board
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Identification apparatus is shot the utility model discloses a kind of microbial morphology, belong to microorganism detection field, the device includes casing (16), lighting source (10), uniform light board (11), imageing sensor (12), image pick-up card (13), record identification systems (14), casing (16) inner side is provided with uniform light board (11), and lighting source (10) is provided between uniform light board (11) and casing (16)；Positioned at the top surface of casing (16), imageing sensor (12) and image pick-up card (13), the output end of image pick-up card (13) are connected imageing sensor (12) with record identification systems (14).The utility model apparatus structure is simple and convenient to operate, identifies quick, accurate.
Description
Technical field
The utility model is related to microorganism detection field, and in particular to a kind of microbial morphology shoots identification apparatus.
Background technology
The identification of microorganism plays key player in clinic, is the foundation of relevant disease diagnosis and treatment.In recent years,
Occur in that many new identification technologies and method, such as full-automatic biochemical reaction identification systems, Mass Spectrometric Identification system.Although above-mentioned
Advanced technology has been developed that, but because its equipment price is expensive, complex operation so that traditional microbial morphology
Learn authentication method and still occupy critical role, be the basis of microorganism classification and identification, it is indispensable.
Microbial morphology identification mainly includes culture dish sample and microscopic structures, and culture dish sample is mainly used in seeing
Examine the features such as size, outward appearance, edge configuration, color, gloss, the haemolysis situation of bacterium colony；The micro- life of microscopic structures main detection
The number and form of thing individual size, shape, arrangement, dyeability, mycelia and spore, whether there is the spies such as brood cell, pod membrane and flagellum
Different structure, and further combined with biochemical, mass spectrum property, identification classification is carried out to microorganism with this.
In medical photography, microbial morphology is the important object for shooting, and whether the true of its feature instantiation, protrusion are
Weigh the standard of shooting quality, and shooting condition (such as color of light source, light angle, luminous intensity, shooting angle, image sensing
Device etc.) directly influence the quality of microbial morphology picture.
Chinese invention patent application 104561229A disclosed culture and the shape of a kind of nanometer bacteria on April 29th, 2015
State authentication method；2010《Jilin University》Disclosed in the Ph.D. Dissertation of the fourth skin of bamboo " based on biotechnology and calculating
The food microorganisms quick detection research of machine vision ".But not yet retrieve is carried out in the prior art to microbial culture dish form
The relevant device for shooting and identifying.
The content of the invention
The purpose of this utility model is, there is provided a kind of microbial morphology shoots identification apparatus, and the apparatus structure is simple, behaviour
Make convenient, identification quick, accurate.
To achieve the above object, the technical solution of the utility model is as follows：
A kind of microbial morphology shoots identification apparatus, and the device includes casing 16, lighting source 10, uniform light board 11, image
Sensor 12, image pick-up card 13, record identification systems 14, wherein, the side of the casing 16 is provided with opening, and the inner side of casing 16 sets
There is uniform light board 11, lighting source 10 is provided between uniform light board 11 and casing 16；The top surface of casing 16 is provided with through hole, imageing sensor
12 top surfaces for being located at casing 16, and it is corresponding with through hole, imageing sensor 12 is connected with the input of image pick-up card 13, image
The output end of capture card 13 is connected with the input of record identification systems 14.
The brightness of the lighting source 10 and Color tunable；The shooting angle of described image sensor 12 is adjustable.
The cross section of the uniform light board 11 is circular arc or rectangle.
The lighting source 10 is LED, incandescent lamp, Halogen lamp LED or fluorescent lamp.
The uniform light board 11 is lucite, polyformaldehyde or nylon.
The connected mode of described image sensor 12 and image pick-up card 13, image pick-up card 13 and record identification systems 14
It is wired connection or wireless connection.
The beneficial effects of the utility model are：
Microbial morphology of the present utility model shoots identification apparatus by adjustable brightness and the lighting source of color, even
Under the reflection and refraction action of tabula rasa, by direct irradiation and back lighting, using the imageing sensor of adjustable shooting angle,
The image pick-up card of wired or wireless connection shoots the spies such as size, outward appearance, edge configuration, color, gloss, the haemolysis situation of bacterium colony
Levy, and features described above is identified using identification systems recorded, in combination with the identification of means such as mass spectrum, biochemistry realize it is quick,
Precise Identification.
Brief description of the drawings
Fig. 1 is the structural representation that microbial morphology of the present utility model shoots identification apparatus.
Fig. 2 is the partial schematic diagram that microbial morphology of the present utility model shoots identification apparatus.
Reference
10 lighting sources
11 uniform light boards
12 imageing sensors
13 image pick-up cards
14 record identification systems
15 microbial culture dishs
16 casings
Specific embodiment
Below in conjunction with the accompanying drawings, specific embodiment of the present utility model is described in further detail.
Microbial morphology of the present utility model shoots identification apparatus and is acquired by microbial morphology, and with calculating
The microbial morphology picture of acquisition is carried out feature extraction and pattern-recognition by machine vision technique, is carried out with reference to biochemical, mass spectrography micro-
Biological Rapid identification.
As shown in Figure 1-2, microbial morphology of the present utility model shoots identification apparatus including casing 16, for illuminating micro- life
The brightness of thing form and the lighting source 10 of Color tunable, the uniform light board 11 for uniform protection lighting source 10, for clapping
Take the photograph the adjustable imageing sensor 12 of shooting angle of microbial morphology and obtain the image pick-up card 13 of transmission view data, be used for
The record identification systems 14 of the utilization computer vision technique of record and identification microbial morphology.
The side of casing 16 is provided with opening, and for picking and placeing microbial culture dish 15, the inner side of casing 16 is provided with uniform light board 11, even light
Lighting source 10 is provided between plate 11 and casing 16；The top surface of casing 16 is provided with through hole, and imageing sensor 12 is located at casing 16
Top surface, and it is corresponding with through hole, described image sensor 12 can carry out global and details and shoot by optics and numeral amplification；
Imageing sensor 12 is connected with the input of image pick-up card 13, output end and the record identification systems 14 of image pick-up card 13
Input is connected.
The cross section of the uniform light board 11 is circular arc or rectangle.
The lighting source 10 is preferably LED, incandescent lamp, Halogen lamp LED or fluorescent lamp, and lighting source 10 can be provided directly
Irradiation and back lighting are connect, distribution angle is in circular arc or rectangle, can carry out brightness and color is adapted to different cultures
The shooting of different colonial morphologies on base.
The uniform light board 11 is preferably lucite, polyformaldehyde or nylon, and uniform light board 11 can be by lighting source 10
The refraction and reflection for emitting beam make maintaining uniform illumination.
The connected mode of described image sensor 12 and image pick-up card 13, image pick-up card 13 and record identification systems 14
It is wired connection or wireless connection.For example, wired connection mode can be connected using usb protocol, sent out by recording identification systems 14
The instruction for obtaining image is sent, imageing sensor 12 is exposed and is imaged, modulus is carried out in the presence of image pick-up card 13 and is turned
Change, and digital information is sent to record identification systems 14 by USB connecting lines, complete IMAQ.Wireless mode can then lead to
WIFI agreements are crossed, specific gatherer process is consistent with above-mentioned wired connection mode.
Microbial morphology of the present utility model shoots authentication method and comprises the following steps：
1) microbial culture dish 15 that will fill microbe colony is positioned over the bottom of casing 1, opens lighting source 1, will
Brightness and color adaptation are to desired value, and the light that lighting source 1 sends is micro- by being irradiated to after the reflection and refraction of equal tabula rasa 11
On bacterium colony in biological culture dish 15；
The mode based on regulation electric current or regulation voltage is carried out according to the characteristics of lighting source 10 to be illuminated light source 10 and send out
The strong and weak control of light.For example, LED light source is driven using current constant mode, and electric current regulation, Jin Ergai are carried out by circuits such as amplifiers
Become luminous intensity；Incandescent lamp, by changing the angle of flow of IGCT, makes to be added to day by the SCR light regulation circuit with rectifier bridge
The power frequency ac voltage at light lamp tube two ends changes, and then changes luminous intensity.
2) imageing sensor 12 is opened, to colonial morphology (such as size, outward appearance, edge shape in microbial culture dish 15
The features such as state, color, gloss, haemolysis situation) it is acquired；
3) colonial morphology that will be collected is sent to image pick-up card 13, and is further conveyed in record identification systems 14
Recorded and identified, the separation of bacterium colony and background, i.e. carrying out image threshold segmentation are carried out according to gray feature；Followed by image side
Edge extracts the feature extraction that colonial morphology is carried out with gray scale morphology scheduling algorithm；Finally using neutral net, SVMs etc.
Algorithm is classified and is retrieved to current feature, carries out the differentiation of strain.
Embodiment 1
Embodiment 1 is by taking the shooting of EHEC (Escherichia coli) and identification as an example.
EHEC belongs to Escherichia, is aerobic or facultative anaerobic bacteria, and 35 DEG C of incubations 24 are small on blood agar plate
When, the microbial culture dish 15 that will fill EHEC is placed on microbial morphology shooting identification apparatus of the present utility model
In, lily lighting source 10 is set to by the setting brightness 80%, color of circular arc, in the uniform light board 11 of lucite
Reflection and refraction action under, direct irradiation and back lighting by lighting source 10, using adjusting shooting angle for 90 degree
Imageing sensor 12, by WIFI wireless connections image pick-up card 13 record microbial culture dish 15 in bacterium colony size,
The features such as outward appearance, edge configuration, color, gloss, haemolysis situation, and features described above is recorded by recording identification systems 14
And identification, according to the colony characteristicses of EHEC, you can form a diameter of 2~3mm, slightly convex, circular, moistening, canescence, side
Edge is neat, opaque colony, while mass spectrum and biochemical identification can be combined, judges that this bacterium is EHEC, completes to shoot and reflects
It is fixed.
Specific embodiment of the present utility model is above are only, but design concept of the present utility model is not limited thereto,
All changes for carrying out unsubstantiality to the utility model using this design, all should belong to the row for invading the utility model protection domain
For.
Claims (6)
1. a kind of microbial morphology shoots identification apparatus, it is characterised in that：
The device include casing (16), lighting source (10), uniform light board (11), imageing sensor (12), image pick-up card (13),
Record identification systems (14), wherein,
Casing (16) side is provided with opening, and casing (16) inner side is provided with uniform light board (11), uniform light board (11) and casing (16)
Between be provided with lighting source (10)；The top surface of casing (16) is provided with through hole, imageing sensor (12) positioned at casing (16) top surface,
And it is corresponding with through hole, imageing sensor (12) is connected with the input of image pick-up card (13), image pick-up card (13) it is defeated
Go out end to be connected with the input of record identification systems (14).
2. microbial morphology according to claim 1 shoots identification apparatus, it is characterised in that：
The brightness of the lighting source (10) and Color tunable；
The shooting angle of described image sensor (12) is adjustable.
3. microbial morphology according to claim 1 shoots identification apparatus, it is characterised in that：
The cross section of the uniform light board (11) is circular arc or rectangle.
4. microbial morphology according to claim 1 shoots identification apparatus, it is characterised in that：
The lighting source (10) is LED, incandescent lamp, Halogen lamp LED or fluorescent lamp.
5. microbial morphology according to claim 1 shoots identification apparatus, it is characterised in that：
The uniform light board (11) is lucite, polyformaldehyde or nylon.
6. microbial morphology according to claim 1 shoots identification apparatus, it is characterised in that：
The connection of described image sensor (12) and image pick-up card (13), image pick-up card (13) and record identification systems (14)
Mode is wired connection or wireless connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621254188.3U CN206204318U (en) | 2016-11-19 | 2016-11-19 | A kind of microbial morphology shoots identification apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621254188.3U CN206204318U (en) | 2016-11-19 | 2016-11-19 | A kind of microbial morphology shoots identification apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206204318U true CN206204318U (en) | 2017-05-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621254188.3U Withdrawn - After Issue CN206204318U (en) | 2016-11-19 | 2016-11-19 | A kind of microbial morphology shoots identification apparatus |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106367330A (en) * | 2016-11-19 | 2017-02-01 | 厦门大学 | Shooting identification device and method for morphology of microorganisms |
| CN109087272A (en) * | 2018-10-17 | 2018-12-25 | 天津师范大学 | For acquiring the noise reduction system and application method of simulative medicine twilight image |
| CN111242141A (en) * | 2020-01-18 | 2020-06-05 | 深圳数马电子技术有限公司 | Key tooth shape code identification method and device, storage medium and image acquisition equipment |
| CN112090785A (en) * | 2020-08-27 | 2020-12-18 | 宁波丞达精机有限公司 | Optical lens sorting equipment |
-
2016
- 2016-11-19 CN CN201621254188.3U patent/CN206204318U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106367330A (en) * | 2016-11-19 | 2017-02-01 | 厦门大学 | Shooting identification device and method for morphology of microorganisms |
| CN109087272A (en) * | 2018-10-17 | 2018-12-25 | 天津师范大学 | For acquiring the noise reduction system and application method of simulative medicine twilight image |
| CN111242141A (en) * | 2020-01-18 | 2020-06-05 | 深圳数马电子技术有限公司 | Key tooth shape code identification method and device, storage medium and image acquisition equipment |
| CN112090785A (en) * | 2020-08-27 | 2020-12-18 | 宁波丞达精机有限公司 | Optical lens sorting equipment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170531 Effective date of abandoning: 20181127 |
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| AV01 | Patent right actively abandoned |