CN108823095A - A kind of environmental Kuznets Curves and observation analysis system in living cells incubation - Google Patents
A kind of environmental Kuznets Curves and observation analysis system in living cells incubation Download PDFInfo
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Abstract
The present invention relates to a kind of environmental Kuznets Curves in living cells incubation and observation analysis systems, wherein, electric boiling plate, headlamp and ultraviolet germicidal are provided in cabinet, motion platform is provided with below headlamp, motion platform is arranged with microcobjective, the light that LED light source issues injects light splitting piece by optical fiber beam, enters microcobjective by light a part of light splitting piece, another part enters CCD camera；CCD camera shoots cell by microcobjective timing；Computer analyzes image data, and combines optimal splitting time theoretical value, picks out the maximum cell of potential；Multiple environmental sensors are additionally provided in cabinet, environmental sensor is by the environmental parameter Real-time Feedback of box house to control panel, the environmental parameter that control panel will test is compared with the environmental parameter of setting, and is controlled according to comparison result solenoid valve, blower, electric boiling plate and headlamp.The present invention can Automatic sieve select optimal embryo.
Description
Technical field
The present invention relates to Bioexperiment instrument and equipment technical fields, more particularly to one kind in living cells incubation
Environmental Kuznets Curves and observation analysis system.
Background technique
Traditional carbon dioxide incubator has been widely used for cell tissue, the culture studies of bacterium, can be cell
Culture provide suitable temperature, pressure, illumination and oxygen and carbon dioxide concentration.Currently, to be seen to the cell in culture
It examines, cell need to be taken out from incubator and move to room temperature environment, this not only adds the possibility that environment interferes cell contamination,
Operating error risk is added to experimenter, especially in embryo's screening process.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of environmental Kuznets Curves in living cells incubation and sights
Analysis system is examined, intelligent control can be carried out to the environment in incubator.
The technical solution adopted by the present invention to solve the technical problems is：It provides a kind of in living cells incubation
Environmental Kuznets Curves and observation analysis system, including cabinet and LED light source, there are two air inlet and an outlets for setting on the cabinet
Mouthful, solenoid valve and blower are provided on the gas outlet and two air inlets, an air inlet is used in described two air inlets
In transport of carbon dioxide, another air inlet is for conveying oxygen；Electric boiling plate, headlamp and ultraviolet are provided in the cabinet
Bactericidal lamp, the headlamp lower section are provided with the motion platform being connected with computer, and the motion platform is arranged with micro- object
Mirror, the light that the LED light source issues inject light splitting piece by optical fiber beam, described in light a part entrance by light splitting piece
Microcobjective, another part enter the CCD camera being connected with computer；The CCD camera observes position by microcobjective in real time
In the Growth trends of each embryo on motion platform, and timing shoots cell；The computer is by Embryo Culture
Process is divided into several stages, and analyzes with intelligent algorithm realization image data, raw to the embryo in each stage
It is long to provide scoring, and optimal splitting time theoretical value is combined, pick out the maximum cell of potential；It is additionally provided in the cabinet more
A environmental sensor, the environmental sensor are connected with control panel, the control panel also with solenoid valve, wind it is mechanical, electrical plus
Hot plate, headlamp are connected with ultraviolet germicidal；The environmental sensor is by the environmental parameter Real-time Feedback of box house to control
Panel, the environmental parameter that the control panel will test are compared with the environmental parameter of setting, and according to comparison result pair
Solenoid valve, blower, electric boiling plate and headlamp are controlled.
Even photosystem is additionally provided between the optical fiber beam and light splitting piece, the even photosystem issues LED light source
Light become directional light.
The environmental sensor includes optical sensor, pressure transducer, temperature sensor, electrochemical oxygen sensor and red
Outside line carbon dioxide sensor.
Central controller when temperature sensor detects that the intracorporal temperature of case is higher than desired temperature, on control panel
The electric boiling plate is closed, when temperature sensor detects the intracorporal temperature of case lower than desired temperature, on control panel
Central controller opens the electric boiling plate；When pressure transducer detects that the intracorporal pressure of case is greater than pressure settings, control
Central controller on panel processed closes solenoid valve and blower on two air inlets, opens the electromagnetic valve and wind on gas outlet
The intracorporal extra gas of case is discharged in machine, when pressure transducer detects that the intracorporal pressure of case is less than pressure settings, then closes
The solenoid valve and blower on gas outlet are closed, solenoid valve and blower on two air inlets are opened；When infrared ray carbon dioxide sensing
When the gas concentration lwevel that device detects is more than gas concentration lwevel setting value, the central controller on control panel opens conveying
Solenoid valve and blower on the air inlet of oxygen open simultaneously solenoid valve and blower on gas outlet, reduce the intracorporal dioxy of case
Change concentration of carbon；Center control when light intensity sensor detects that the intracorporal luminous intensity of case is higher than light intensity setting value, on control panel
Device processed reduces the brightness of headlamp, when light intensity sensor detects the intracorporal luminous intensity of case lower than light intensity setting value, control plane
The brightness of headlamp is turned up in central controller on plate.
Central controller on the control panel periodically opens ultraviolet germicidal for sterilizing to box house.
Pneumatic filter is provided on two air inlets.
The computer uses intelligent algorithm realization to carry out analysis to image data, and the specific method is as follows：Acquisition is different
The embryonic cell image of fixed period；In conjunction with clinical expert evaluation method, scoring mark is designed to different periods embryonic cell image
Remember module；Mark scoring image data with the convolutional Neural of deep learning technology training bottom according to clinical expert, study can
Then the structure of discrimination is trained different scoring images on high-rise convolutional Neural, thus for specific cell point
Change problem using less training image identification and the embryonic differentiation associated image specific structure, when finally will be each fixed
The scoring of section, which is accumulated to subsequent period, obtains final score.
Beneficial effect
Due to the adoption of the above technical solution, compared with prior art, the present invention having the following advantages that and actively imitating
Fruit：The present invention carries out the indoor environment of chamber by multiple indoor environment of sensor test chamber, and according to the numerical value detected
It adjusts in real time, guarantees that embryo's (observing without moving on in outdoor environment) in optimum chamber develops, while observing each
The Growth trends of embryo can periodically shoot cell by built-in inversion microcobjective, by Embryo Culture process point
For several stages, realizes with artificial intelligence to analysis of image data function, scoring is provided to the embryo growth in each stage, most
It is optimal splitting time theoretical value that accumulation scoring is highest afterwards, picks out the maximum embryo quality of potential, automatic screening is unqualified
Embryo reduces embryo caused by one man operation and screens error, and transplanting parent improves list and matches implantation rate, greatly reduces workload.
Detailed description of the invention
Fig. 1 is appearance diagram figure of the invention；
Fig. 2 is the structural schematic diagram of cabinet in the present invention；
Fig. 3 is control principle schematic diagram of the invention.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, those skilled in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Range.
Embodiments of the present invention are related to a kind of environmental Kuznets Curves in living cells incubation and observation analysis system,
As depicted in figs. 1 and 2, including cabinet 1 and LED light source 19, there are two air inlet and a gas outlets for setting on the cabinet 1
18, solenoid valve and blower 12, an air inlet in described two air inlets are provided on the gas outlet 18 and two air inlets
Mouth 15 is used for transport of carbon dioxide, another air inlet 14 is provided with gas filtration on two air inlets for conveying oxygen
Device 13；Electric boiling plate 10, headlamp 4 and ultraviolet germicidal 2 are provided in the cabinet 1, be provided with below the headlamp 4 with
The connected motion platform 9 of computer, the motion platform 9 are arranged with microcobjective 17, the light that the LED light source 19 issues
By optical fiber beam 20 inject light splitting piece, by light splitting piece light a part enter the microcobjective 17, another part into
Enter the CCD camera 11 being connected with computer；Even photosystem 16 is additionally provided between the optical fiber beam 20 and light splitting piece, it is described even
The light that photosystem 16 issues LED light source 19 becomes directional light；Multiple environmental sensors are additionally provided in the cabinet 1,
The environmental sensor is connected with control panel 22, the control panel 22 also with solenoid valve, blower 12, electric boiling plate 10,
Headlamp 4 is connected with ultraviolet germicidal 2；The environmental sensor is by the environmental parameter Real-time Feedback of box house to control plane
Plate, the environmental parameter that the control panel 22 will test are compared with the environmental parameter of setting, and according to comparison result pair
Solenoid valve, blower 12, electric boiling plate 10 and headlamp 4 are controlled.In present embodiment, the environmental sensor includes that light passes
Sensor 3, pressure transducer 5, temperature sensor 6, electrochemical oxygen sensor 7 and IR carbon dioxide sensors 8.
In present embodiment, the inside of the cabinet 1 is stainless steel material, and inner hollow, top is equipped with ultraviolet sterilization
Lamp 2, headlamp 4, optical sensor 3, pressure transducer 5, temperature sensing/6, electrochemical oxygen sensor 7, IR carbon dioxide
Signal can be transmitted to control panel 22 in time by sensor 8, the sensor, and 1 left and right sides inner wall of cabinet is equipped with electric heating
Plate 10, electric boiling plate 10 are connected with control panel 22, for being adjusted to the temperature in cabinet 1, described two air inlets 14,
15 are mounted on the right side lower end of cabinet 1, are equipped with the blower 12 of inside air-breathing and the pneumatic filter 13 of sterilizing dust removing, gas thereon
Body filter 13 is mounted on the right side of blower 12, and the gas outlet 18 is mounted on upper end on the left of cabinet, is equipped with thereon to outlet
The blower 12 of gas is equipped with electromagnetic valve in air inlet and air outlet, for the adjusting of oxygen in case and carbon dioxide pressure, cabinet
1 inside immediately below be equipped with microcobjective 17 be the long depth of field be inverted microcobjective, amplification factor be higher than 20 ×, the microcobjective 17
Imaging system is constituted with CCD camera 11, the long depth of field, which is inverted above microcobjective, is equipped with motion platform 9, the motion platform 9
Precision is higher than 10 microns, and motion platform 9 is connected with computer, and motion platform 9 is for selection each for supporting a variety of object carrying supports
The orifice plate or multiple culture dishes of kind specification adjust visual field position when can observe cell in real time by adjusting the position of motion platform
It sets.
When this system works normally, each sensor can be by pressure, light intensity, oxygen concentration, the carbon dioxide inside cabinet 1
The parameters such as concentration, temperature Real-time Feedback will test value compared with setting value to control panel 22, by control panel, thus to training
Feeding case is controlled.Wherein, temperature sensor transmits a signal to control panel 22 for the temperature in detection box 1 in real time
On central controller, when the temperature detected is higher than desired temperature, the central controller on control panel 22 closes electricity
Heating plate 10, when the temperature detected is lower than desired temperature, the central controller on control panel 22 opens electric boiling plate
10.Carbon dioxide sensor, oxygen sensor and pressure transducer are for controlling 1 internal gas concentration of cabinet and pressure, in real time
Feed back to the central controller on control panel 22.When the pressure in cabinet 1 is greater than pressure settings, on control panel 22
Central controller can close oxygen air inlet 14 and solenoid valve and blower 12 on carbon dioxide mouth 15, open gas outlet
The extra gas in cabinet 1 is discharged in solenoid valve and blower 12 on 18, conversely, then close solenoid valve on gas outlet 18 and
Blower 12 opens oxygen air inlet 14 and solenoid valve and blower 12 on carbon dioxide mouth 15；When the titanium dioxide detected
When concentration of carbon is more than carbon dioxide setting value, the central controller on control panel 22 opens 14 solenoid valve of oxygen air inlet and wind
Machine 12 opens simultaneously solenoid valve and blower 12 on gas outlet 18, reduces the gas concentration lwevel in cabinet 1.Light intensity sensor
For the luminous intensity in detection box 1, the central controller on control panel 22 is transmitted a signal in real time, when the light detected
When being higher than light intensity setting value by force, the central controller on control panel 22 reduces by 4 brightness of headlamp, when the light intensity detected is higher than
When light intensity setting value, the brightness of headlamp 4 is turned up in the central controller on control panel 22；Ultraviolet germicidal 2 is for periodically right
1 inner sterilization of cabinet, the internal environment of strict control cabinet 1.
This system carries out soft fast imaging using near-infrared transmission light LED, and compatible various cell culture containers own
Accurately hardware trigger and fast illumination may be implemented in LED, greatly reduces phototoxicity, and LED light source is passed through again by the transmission of optical fiber 20
Light splitting piece is to the light path system of object lens, and entire light path system sealing waterproof, motion platform 9 is connected with computer, containing more
Chamber cell culture chamber, computer can control the movement of motion platform 9, adjust field positions, and CCD camera 11 is fallen with the long depth of field
Microcobjective is set to be connected, can be by the cell picture transmission observed in real time to computer, computer uses intellectual analysis software
Data analysis function, time point in cell division period is annotated, in conjunction with optimal splitting time theoretical value, picks out potential most
Big cell, the transplanting for the later period.
Laboratory need to observe the mitosis during Embryo Culture when carrying out embryo transfer.This system can be real-time
Imaging guarantees that embryo while (observing without moving on in outdoor environment) development, observes each embryo in optimum chamber
The Growth trends of tire can periodically shoot cell by built-in inversion microcobjective, Embryo Culture process is divided into
Several stages are realized to analysis of image data function with artificial intelligence, provide scoring to the embryo growth in each stage, finally
Highest accumulation scoring is optimal splitting time theoretical value, picks out the maximum embryo quality of potential, the unqualified embryo of automatic screening
Tire reduces embryo caused by one man operation and screens error, and transplanting parent improves list and matches implantation rate, greatly reduces workload.Its
In, specific analytical method is as follows：1. acquiring the embryonic cell image of different fixed periods；2. clinical expert evaluation method is combined,
Scoring mark module is designed to different periods embryo's image；3. marking scoring image data to use deep learning according to clinical expert
The convolutional Neural of technique drill bottom learns recognizable structure, then to different scoring images on high-rise convolutional Neural
Be trained, so as to use less training image for specific cell differentiation problem, identification faster, more efficient with
The scoring of each fixed period is finally accumulated to subsequent period and is obtained finally by the specific structure of the embryonic differentiation associated image
Score, 60 points can be arranged according to characteristics of image the following are unqualified, which period no matter arrive, accumulation scoring is just said lower than 60 points
This bright embryonic differentiation is severe, and score highest is then optimum embryo, and transplanting has higher success rate；4. visual using Attention etc.
Change analytical technology to check how algorithm is predicted, these technologies produce a thermal map, show which pixel or image are special
Structure is levied for predicting that specific differentiation is mostly important, clinical expert is helped to generate more targeted hypothesis；5. with sample number
Increase, system can continue to optimize automatically correct its accuracy, realize iteration upgrading.
It is not difficult to find that the present invention is by multiple indoor environment of sensor test chamber, and according to the numerical value detected to chamber
Indoor environment is adjusted in real time, guarantees that embryo's (observing without moving on in outdoor environment) in optimum chamber develops,
The Growth trends for observing each embryo simultaneously can periodically shoot cell by built-in inversion microcobjective, will
Embryo Culture process is divided into several stages, realizes with artificial intelligence to analysis of image data function, to the embryo in each stage
Growth provides scoring, and highest finally accumulation scoring is optimal splitting time theoretical value, picks out the maximum embryo quality of potential,
The unqualified embryo of automatic screening reduces embryo caused by one man operation and screens error, and transplanting parent improves list and matches implantation rate, significantly
Reduce workload.
Claims (7)
1. a kind of environmental Kuznets Curves and observation analysis system in living cells incubation, including cabinet and LED light source, special
Sign is, setting is respectively provided with there are two air inlet and a gas outlet on the gas outlet and two air inlets on the cabinet
There are solenoid valve and blower, an air inlet is used for transport of carbon dioxide in described two air inlets, another air inlet is for defeated
Send oxygen；It is provided with electric boiling plate, headlamp and ultraviolet germicidal in the cabinet, is provided with and calculates below the headlamp
The connected motion platform of machine, the motion platform are arranged with microcobjective, and the light that the LED light source issues passes through optical fiber light
Beam enters light splitting piece, enters the microcobjective by light a part of light splitting piece, another part enters and is connected with computer
CCD camera；The CCD camera is dynamic by the growth that microcobjective observes each embryo being located on motion platform in real time
State, and timing shoots cell；Embryo Culture process is divided into several stages by the computer, and is calculated with artificial intelligence
Image data is analyzed in method realization, provides scoring to the embryo growth in each stage, and combine optimal splitting time theoretical
Value, picks out the maximum cell of potential；Be additionally provided with multiple environmental sensors in the cabinet, the environmental sensor with control
Panel processed is connected, and the control panel is also connected with solenoid valve, blower, electric boiling plate, headlamp and ultraviolet germicidal；The ring
The environmental parameter that border sensor will test the environmental parameter Real-time Feedback of box house to control panel, the control panel
It is compared with the environmental parameter of setting, and solenoid valve, blower, electric boiling plate and headlamp is controlled according to comparison result
System.
2. the environmental Kuznets Curves and observation analysis system according to claim 1 in living cells incubation, feature
It is, even photosystem, the light that the even photosystem issues LED light source is additionally provided between the optical fiber beam and light splitting piece
Line becomes directional light.
3. the environmental Kuznets Curves and observation analysis system according to claim 1 in living cells incubation, feature
It is, the environmental sensor includes optical sensor, pressure transducer, temperature sensor, electrochemical oxygen sensor and infrared
Line carbon dioxide sensor.
4. the environmental Kuznets Curves and observation analysis system according to claim 3 in living cells incubation, feature
It is, when temperature sensor detects that the intracorporal temperature of case is higher than desired temperature, the central controller on control panel is closed
Close the electric boiling plate, when temperature sensor detects the intracorporal temperature of case lower than desired temperature, on control panel in
Controller is entreated to open the electric boiling plate；When pressure transducer detects that the intracorporal pressure of case is greater than pressure settings, control
Central controller on panel closes solenoid valve and blower on two air inlets, opens the electromagnetic valve and wind on gas outlet
The intracorporal extra gas of case is discharged in machine, when pressure transducer detects that the intracorporal pressure of case is less than pressure settings, then closes
The solenoid valve and blower on gas outlet are closed, solenoid valve and blower on two air inlets are opened；When infrared ray carbon dioxide sensing
When the gas concentration lwevel that device detects is more than gas concentration lwevel setting value, the central controller on control panel opens conveying
Solenoid valve and blower on the air inlet of oxygen open simultaneously solenoid valve and blower on gas outlet, reduce the intracorporal dioxy of case
Change concentration of carbon；Center control when light intensity sensor detects that the intracorporal luminous intensity of case is higher than light intensity setting value, on control panel
Device processed reduces the brightness of headlamp, when light intensity sensor detects the intracorporal luminous intensity of case lower than light intensity setting value, control plane
The brightness of headlamp is turned up in central controller on plate.
5. the environmental Kuznets Curves and observation analysis system according to claim 1 in living cells incubation, feature
It is, the central controller on the control panel periodically opens ultraviolet germicidal for sterilizing to box house.
6. the environmental Kuznets Curves and observation analysis system according to claim 1 in living cells incubation, feature
It is, is provided with pneumatic filter on two air inlets.
7. the environmental Kuznets Curves and observation analysis system according to claim 1 in living cells incubation, feature
Be, the computer using intelligent algorithm realize to image data carry out analysis the specific method is as follows：Acquisition is different solid
The embryonic cell image of timing section；In conjunction with clinical expert evaluation method, scoring label is designed to different periods embryonic cell image
Module；Scoring image data is marked to learn distinguishable with the convolutional Neural of deep learning technology training bottom according to clinical expert
Then other structure is trained different scoring images on high-rise convolutional Neural, to be directed to specific cell differentiation
Problem recognizes the specific structure with the embryonic differentiation associated image using less training image, finally by each fixed period
Scoring accumulate and to subsequent period obtain final score.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500453A (en) * | 2020-04-30 | 2020-08-07 | 龙岩学院 | Sealed cultivation device for biological cell cultivation and use method thereof |
| CN112662551A (en) * | 2020-12-29 | 2021-04-16 | 上海药明生物医药有限公司 | Cell culture control method and system |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174396A (en) * | 2011-03-02 | 2011-09-07 | 暨南大学 | Box real-time living cell culture recording system |
| CN102289066A (en) * | 2011-08-12 | 2011-12-21 | 北京航空航天大学 | Automatic microscopic imaging system for multicellutar culture course |
| CN104411817A (en) * | 2012-06-25 | 2015-03-11 | 尤尼森斯繁殖技术公司 | Method and apparatus |
| CN104774906A (en) * | 2015-04-24 | 2015-07-15 | 南京爱贝生物科技有限公司 | Continuous observation method for living cells |
| CN105408746A (en) * | 2013-02-28 | 2016-03-16 | 普罗吉涅股份有限公司 | Apparatus, method, and system for image-based human embryo cell classification |
| CN205313575U (en) * | 2016-01-17 | 2016-06-15 | 南阳师范学院 | Carbon dioxide incubator |
| CN106202997A (en) * | 2016-06-29 | 2016-12-07 | 四川大学 | A kind of cell division detection method based on degree of depth study |
| KR20170019049A (en) * | 2015-08-11 | 2017-02-21 | 을지대학교 산학협력단 | Time lapse incubator |
| CN106485251A (en) * | 2016-10-08 | 2017-03-08 | 天津工业大学 | Egg embryo classification based on deep learning |
| CN206783690U (en) * | 2017-06-02 | 2017-12-22 | 江苏蒙彼利生物科技有限公司 | New NK cell culture CO2gas incubator |
| CN206872848U (en) * | 2017-04-10 | 2018-01-12 | 中国人民解放军总医院 | A kind of cell culture incubator |
-
2018
- 2018-06-08 CN CN201810586766.0A patent/CN108823095A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174396A (en) * | 2011-03-02 | 2011-09-07 | 暨南大学 | Box real-time living cell culture recording system |
| CN102289066A (en) * | 2011-08-12 | 2011-12-21 | 北京航空航天大学 | Automatic microscopic imaging system for multicellutar culture course |
| CN104411817A (en) * | 2012-06-25 | 2015-03-11 | 尤尼森斯繁殖技术公司 | Method and apparatus |
| CN105408746A (en) * | 2013-02-28 | 2016-03-16 | 普罗吉涅股份有限公司 | Apparatus, method, and system for image-based human embryo cell classification |
| CN104774906A (en) * | 2015-04-24 | 2015-07-15 | 南京爱贝生物科技有限公司 | Continuous observation method for living cells |
| KR20170019049A (en) * | 2015-08-11 | 2017-02-21 | 을지대학교 산학협력단 | Time lapse incubator |
| CN205313575U (en) * | 2016-01-17 | 2016-06-15 | 南阳师范学院 | Carbon dioxide incubator |
| CN106202997A (en) * | 2016-06-29 | 2016-12-07 | 四川大学 | A kind of cell division detection method based on degree of depth study |
| CN106485251A (en) * | 2016-10-08 | 2017-03-08 | 天津工业大学 | Egg embryo classification based on deep learning |
| CN206872848U (en) * | 2017-04-10 | 2018-01-12 | 中国人民解放军总医院 | A kind of cell culture incubator |
| CN206783690U (en) * | 2017-06-02 | 2017-12-22 | 江苏蒙彼利生物科技有限公司 | New NK cell culture CO2gas incubator |
Non-Patent Citations (3)
| Title |
|---|
| MATOS ET AL.: "A method using artificial neural networks to morphologically assess mouse blastocyst quality", 《JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY》 * |
| 李建华等: "时差成像监测在评估胚胎发育潜能中的作用", 《中国优生与遗传杂志》 * |
| 赵卫等: "《超高时空分辨多维信息获取技术及其应用》", 30 November 2016, 国防工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500453A (en) * | 2020-04-30 | 2020-08-07 | 龙岩学院 | Sealed cultivation device for biological cell cultivation and use method thereof |
| CN112662551A (en) * | 2020-12-29 | 2021-04-16 | 上海药明生物医药有限公司 | Cell culture control method and system |
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