CN201403420Y

CN201403420Y - Magnetic suspension aquatic organism cultivation device

Info

Publication number: CN201403420Y
Application number: CN200920032465XU
Authority: CN
Inventors: 尹大川; 王玺凯; 卢慧甍; 郭卫红; 商澎
Original assignee: Northwestern Polytechnical University
Current assignee: Northwestern Polytechnical University
Priority date: 2009-04-03
Filing date: 2009-04-03
Publication date: 2010-02-17
Anticipated expiration: 2019-04-03

Abstract

The utility model discloses a magnetic suspension aquatic organism cultivation device which comprises a CCD camera, a suspension cavity and a light source. The magnetic suspension aquatic organism cultivation device is characterized in that the device also comprises light pervious sheets, an ultraviolet light source, a grid filter, a circulating water pipe and a localizer; wherein the suspension cavity is an enclosed cavity which is formed by a wall, an upper light pervious sheet and a lower light pervious sheet, an LED cold light illuminating light source is fixed connected below the lower light pervious sheet, the microminiature CCD camera is arranged above the suspension cavity through the light pervious sheets, optical fibers are fixedly connected around the microminiature CCD camera,the circulating water pipe penetrates the lower light pervious sheet and is connected with two pipe connectors at the bottom of the grid filter in the suspension cavity, the ultraviolet light source is positioned on the wall on one side above the suspension cavity, and the localizer is positioned at the bottom of the wall. Water and nutrition are provided for the suspension organism in the suspension cavity through the supply system formed by the circulating water pipe and the grid filter, so that the suspension organism can survive for a long term, long-term weightless suspension cultivationof living aquatic organism can be realized, and the analog research of the weightless environment can be performed.

Description

Apparatus for magnetic suspension culture of aquatic organisms

Technical field

The utility model relates to a kind of apparatus for magnetic suspension culture, particularly apparatus for magnetic suspension culture of aquatic organisms.

Background technology

With reference to Fig. 3.Document " Takahashi; K.; et al.; Magnetic orientation of paraffin in a magnetic levitationfurnace.Physica B; 2004.346:p.277-281 " discloses a kind of magnetic levitation system, aims at solid suspension 5 and melts to solidify under suspended state and handle and design.This device comprises wall 1, CCD camera 2, suspension chamber 6, light source 9 and speculum 14, wall 1 is the cylindrical structure of inserting magnet, wall 1 built-in several speculums 14, light source 9 is a YAG laser, the YAG laser of light source 9 emissions, after the reflection of speculum 14 minute surfaces, can shine on the suspension 5, by the state of the small ccd camera 2 Real Time Observation suspensions 5 on wall 1 sidewall.

Though this device also can make biologies such as apple, rice be in suspended state, yet owing to do not possess water, gas, the continuous circulation supply system of nutrition; Suspension is in the release position, vulnerable to pollution, and volatile; Adopt the mirror mirror reflection to obtain the suspension image, make the restriction of factors such as suspension picture quality is relatively poor, can not carry out long-time magnetic suspension to the living animal suspension and cultivate.

Summary of the invention

Do not possess better seal in order to overcome existing apparatus, can not obtain the high image of quality and can not carry out the deficiency that long-time magnetic suspension is cultivated the living animal suspension.The utility model provides a kind of apparatus for magnetic suspension culture of aquatic organisms, by water, gas, the continuous circulation supply system of nutrition and in conjunction with microminiature CCD, just can realize that living animal is carried out magnetic suspension to be cultivated, and can realize experimentation is carried out Real Time Observation and obtains best image information.

The technical scheme that its technical problem that solves the utility model adopts: a kind of apparatus for magnetic suspension culture of aquatic organisms, comprise the CCD camera, the suspension chamber, light source, be characterized in also comprising light transmission piece, ultraviolet source, the grid filter, circulating water pipe and positioner, described suspension chamber is an airtight chamber, constitute by wall and light transmission piece up and down, following light transmission piece below is fixed with LED cold light illumination light source, the top in suspension chamber is provided with microminiature CCD camera by light transmission piece, be fixed with optical fiber around the microminiature CCD camera, circulating water pipe passes down light transmission piece and is connected with two pipe interfaces of grid filter bottom in the suspension chamber, ultraviolet source is positioned on the wall of top, suspension chamber one side, and positioner is positioned at the wall bottom.

The beneficial effects of the utility model are: the feed system of forming by circulating water pipe and grid filter provides the life support material of aqueous vapor, nutrition to testing biology, make the experiment biology can carry out the research of Simulated Weightlessness for a long time, realized the long-time weightless suspension culture of live body aquatile; By adopting optical fibre illumination, provide the natural daylight light source; , comprise that magnetizing force that biological sample produces can offset the characteristics of gravity and come Simulated Weightlessness, thereby realize the research of live body aquatile Simulated Weightlessness diamagnetic substance by big strong magnetic field gradient that superconducting magnet possessed; Luminous by the scattering of adopting ultraviolet source, can realize fluorescence molecule mark in the hydrobiological body of live body is carried out Real Time Observation, this function will become the favourable instrument of biological experiment in the magnet; By adopting 10 pairs of suspension chambeies of hygrosensor to monitor in real time, can realize accuracy control over temperature; By the video acquisition system that adopts microminiature CCD camera and lighting source, ultraviolet source and optical fiber to form, characteristic per sample adopts best light source condition, can obtain the excellent images quality.

Below in conjunction with drawings and Examples the utility model is elaborated.

Description of drawings

Fig. 1 is the utility model apparatus for magnetic suspension culture of aquatic organisms structural representation.

Fig. 2 is the enlarged drawing in suspension chamber among Fig. 1.

Fig. 3 is a background technology magnetic levitation system structural representation.

Among the figure: 1-wall, 2-CCD camera, 3-light transmission piece, 4-ultraviolet source, 5-suspension, 6-suspension chamber, 7-grid filter, 8-circulating water pipe, 9-lighting source, 10-hygrosensor, 11-optical fiber, 12-positioner, 13-spiral interface, 14-speculum.

Embodiment

With reference to Fig. 1, Fig. 2.The utility model apparatus for magnetic suspension culture of aquatic organisms comprises wall 1, microminiature CCD camera 2, light transmission piece 3, ultraviolet source 4, suspension chamber 6, grid filter 7, circulating water pipe 8, lighting source 9, hygrosensor 10 and positioner 12.

Superconducting magnet is fixed on the level ground, its center is the cylinder shape cavity body, diameter is 50mm, be about 1210mm, apparatus for magnetic suspension culture of aquatic organisms is packed into by the bottom in the superconducting magnet, adjusts suspensions 5 present positions by the positioner 12 that is fixed on the superconducting magnet wall 1, make its magnetizing force direction suffered upwards in this position, size equates with gravity, thereby counteracting gravity makes it be in simulated weightlessness.

The utility model device wall 1 all need adopt nonferromugnetic material, comprise epoxy resin, copper, aluminium etc., wall 1 is divided into two parts up and down, and light transmission piece 3 is all inlayed by seccotine in inside, wall 1 top and the bottom, and linking to each other by spiral interface 13 forms airtight suspension chamber 6.

Suspension chamber 6 provides airtight no container environment for suspension 5, it is an airtight chamber, there is light transmission piece 36 upper ends, suspension chamber, its top is microminiature CCD camera 2 and optical fiber 11, microminiature CCD camera 2 is positioned at optical fiber 11 central authorities, both position relative fixed, and optical fiber 11 adopts common biography light optical fiber, like this by collective optics highly effective gathering sunshine, under not carrying out the prerequisite that energy transforms, simulate illumination in to suspension chamber 6; Microminiature CCD camera 2 by light transmission piece 3 can 6 inside, Real Time Observation suspension chamber suspension 5; There is light transmission piece 36 lower ends, suspension chamber, the lighting source 9 of its light transmission piece 3 belows is fixed in the bottom of light transmission piece 3 by seccotine, lighting source 9 is selected the LED cold light source of adjustable brightness for use, this device is a light emitting diode, can provide projection light for observing suspension 5 like this, and can not influence the temperature in the suspension chamber 6 during this light source works.

But the internal diameter of circulating water pipe 8 employing high-temperature sterilizations is 1 millimeter a teflon hose, and the peristaltic pump outer with superconducting magnet links to each other, and water circulation is provided, and the flow rate of liquid of water circulation is set by peristaltic pump; Article two, flexible pipe enters suspension chamber 6 and is connected with grid filter 7 from the light transmission piece 3 of 6 belows, suspension chamber, and grid filter 7 can adopt the spun yarn net as filter course, and two pipe interfaces are arranged at its bottom, and interface is connected to form EGR with circulating water pipe 8 insertions; Grid filter 7 in the suspension chamber 6 plays the effect of avoiding suspended biological to pass through, and can be as placing this biological platform before the experiment; Suspension 5 is positioned at above the grid filter 7.

Hygrosensor 10 is positioned on the 6 bottom sidewalls of suspension chamber, and transmission line links to each other with the calculator of periphery by the light transmission piece 3 of 6 bottoms, suspension chamber, the real time temperature in the monitoring suspension chamber 6; Hygrosensor 10 adopts elements such as RTD or thermocouple.Ultraviolet source 4 is positioned on the wall 1 of 6 tops, suspension chamber, one side, and power line is connected with the power supply of periphery by the light transmission piece 3 at 6 tops, suspension chamber; Ultraviolet source 4 adopts mercury lamp equal excitation light source.Positioner 12 is positioned at the bottom of wall 1, by regulating the height of positioner 12, can adjust the levitation position of suspension chamber 6 in superconducting magnet.

Can be suspended in the airtight suspension chamber 6 as aquatiles such as turbellarian worms, realize the long-time existence under no container and the Simulated Weightlessness, and experimentation is monitored in real time, it can be taken out laggard line correlation after experiment finishes and detect and analyze.Operation principle is: with suspension 5-turbellarian worm, be positioned on the grid filter 7 in the suspension chamber 6, connect by the top and the bottom of spiral interface 13, insert, regulate positioner 12 to determine the levitation position of suspension 5-turbellarian worm in superconducting magnet from the centre of superconducting magnet bottom with wall 1; Open the power supply and the data wire of lighting source 9, hygrosensor 10 and temperature indicator; By data wire microminiature CCD camera 2 and calculator are connected to form the Real Time Observation system, and observe image in the suspension chamber 6 by calculator; Use syringe to inject water earlier by circulating water pipe 8, enter grid filter 7 in the suspension chamber 6 by flexible pipe, turbellarian worm on it is in the water, and observe water droplets by microminiature CCD camera 2 and become big gradually, but water droplet does not break away from grid filter 7, because grid filter 7 tops are levitation position, so this drop is suspended state.At last that circulating water pipe 8 and superconducting magnet is outer peristaltic pump links to each other, and forms water circulation path, guarantees that suspension 5-turbellarian worm is in the middle of the circulating water.

Claims

1, a kind of apparatus for magnetic suspension culture of aquatic organisms, comprise the CCD camera, the suspension chamber, light source, it is characterized in that: also comprise light transmission piece, ultraviolet source, the grid filter, circulating water pipe and positioner, described suspension chamber is an airtight chamber, constitute by wall and light transmission piece up and down, following light transmission piece below is fixed with LED cold light illumination light source, the top in suspension chamber is provided with microminiature CCD stylus by light transmission piece, be fixed with optical fiber around the microminiature CCD stylus, circulating water pipe passes down light transmission piece and is connected with two pipe interfaces of grid filter bottom in the suspension chamber, ultraviolet source is positioned on the wall of top, suspension chamber one side, and positioner is positioned at the wall bottom.

2, apparatus for magnetic suspension culture of aquatic organisms according to claim 1 is characterized in that: described wall, its material are any of epoxy resin, copper or aluminium.

3, apparatus for magnetic suspension culture of aquatic organisms according to claim 1 is characterized in that: described circulating water pipe, its material is a polytetrafluoroethylene (PTFE).

4, apparatus for magnetic suspension culture of aquatic organisms according to claim 1 is characterized in that: described grid filter is to adopt the spun yarn net as filter course.

5, apparatus for magnetic suspension culture of aquatic organisms according to claim 1 is characterized in that: described ultraviolet source is a mercury lamp.

6, apparatus for magnetic suspension culture of aquatic organisms according to claim 1 is characterized in that: also comprise a hygrosensor that is positioned on the sidewall of bottom, suspension chamber.