CN211576876U - Pollen monitoring device - Google Patents

Pollen monitoring device Download PDF

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Publication number
CN211576876U
CN211576876U CN202020019961.8U CN202020019961U CN211576876U CN 211576876 U CN211576876 U CN 211576876U CN 202020019961 U CN202020019961 U CN 202020019961U CN 211576876 U CN211576876 U CN 211576876U
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China
Prior art keywords
pollen
heating module
heating
rotating device
slide glass
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CN202020019961.8U
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Chinese (zh)
Inventor
戎恺
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Shanghai Kaiqing Intelligent Technology Co ltd
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Shanghai Kaiqing Intelligent Technology Co ltd
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Abstract

The utility model relates to the technical field of environmental monitoring, in particular to a pollen monitoring device; it includes rotating device, rotatory sample platform, slide glass and heating module, rotating device installs on a base, the vertical upwards setting of rotating device's pivot, the pivot top is connected with rotatory sample platform, rotatory sample bench is provided with a plurality of recesses, the recess is circular and its bottom is provided with the through-hole, detachably places slide glass in the recess, be provided with the gelatin layer on the slide glass, be provided with the glycerine layer on the gelatin layer, still install heating module on the base, the heating end of heating module corresponds the recess setting of rotatory sample platform, utilizes the glycerine layer on the slide glass to adsorb pollen granule, then utilizes the heating to make the gelatin melt, and pollen falls into the gelatin, treats that its cooling can accomplish the fixed of pollen granule, facilitates for subsequent photography, count, observation etc..

Description

Pollen monitoring device
Technical Field
The utility model belongs to the technical field of the environmental monitoring technique and specifically relates to pollen monitoring devices.
Background
The conventional collection method at present mainly adopts a gravity sedimentation method to collect pollen, and then adopts a manual microscopic examination method to classify and count the pollen. The manual microscopic examination method is to carefully observe the pollen sample piece amplified by 400 times under a biological optical microscope line by an experienced observer, manually distinguish the pollen particle types, and manually count the number of different pollens and the total number of the pollens. The quality guarantee period of the samples prepared by the microscopic examination method is short, so that the samples cannot be stored, and the samples cannot be counted again if data is lost. In addition, the problem that the sample cannot be counted for multiple times so as to prevent counting errors and the like is also caused.
For example, chinese patent discloses an online monitoring system for pollen in the atmosphere, patent No. 201510731452.1, in which: the system comprises an impact type sampler, an optical microscope, a sampling membrane belt, a transmission rotating shaft, a pneumatic control valve, a CCD camera, a sampling pump and an industrial personal computer; the impact type sampler is connected with the atmospheric environment; the sampling pump is connected with the impact type sampler through a connecting pipe, and the impact type sampler is used for extracting air from a sampling outer pipe of the impact type sampler and collecting pollen particles in the atmosphere on a sampling film; the sampling membrane band is transmitted to the lower part of the optical microscope through system control and is used for observation and analysis; the CCD camera is used for collecting pollen sample image information and transmitting the pollen sample image information to the computer for image analysis.
Above-mentioned technical scheme sample can't be preserved, destroys pollen sample more easily after the rolling of sampling film moreover.
Disclosure of Invention
Not enough to prior art, the utility model provides a pollen monitoring devices is favorable to the save of pollen sample.
The technical scheme of the utility model is that:
pollen monitoring devices, including rotating device, rotatory sample platform, slide glass and heating module, rotating device installs on a base, the vertical upwards setting of rotating device's pivot, the pivot top is connected with rotatory sample platform, rotatory sample bench is provided with a plurality of recesses, the recess is circular and its bottom is provided with the through-hole, detachably places slide glass in the recess, be provided with the gelatin layer on the slide glass, be provided with the glycerine layer on the gelatin layer, still install heating module on the base, the heating terminal of heating module corresponds the recess setting of rotatory sample platform for slide glass can be heated by the heating terminal of heating module when the recess rotates to the top of heating module. During the use, through equipment such as striking sample thief with pollen striking on the slide, the pollen is stuck by the glycerine layer this moment, then rotates rotatory sample platform for the slide in the recess is located heating module top, and heats. After heating, the pollen is sunk into the melted gelatin layer, and after cooling, the fixation of the pollen can be realized. And then rotating the rotary sample table, and observing the pollen sample by using an electron microscope.
In some embodiments, the rotating device employs an indexing disk. The angle of each rotation can be accurately controlled.
In some embodiments, the material of the rotary sample stage is one of a cast iron plate, a stainless steel plate or a high temperature resistant resin plate. Has enough hardness and is not afraid of high temperature.
In some embodiments, the heating module is a far infrared heater, and the heating direction of the heating module is vertically upward. As is known, the far infrared heater has a limited heating effect on an air medium, so most of heat can directly reach the glass slide, the heating effect is improved, the heat loss is reduced, and the air around the device cannot be greatly heated.
The utility model has the advantages that: the glycerol layer on the glass slide is used for adsorbing the pollen particles, then the gelatin is heated to be molten, the pollen falls into the gelatin, and the fixing of the pollen particles can be completed after the pollen is cooled, so that convenience is provided for subsequent photographing, counting, observation and the like; the protection of gelatin can greatly improve the repeated use times of the sample.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of the slide glass of the present invention;
fig. 3 is a schematic view of one of the usage modes of the present invention.
In the figure, 1, a base; 2. a rotating device; 3. a rotating shaft; 4. rotating the sample table; 5. a groove; 6. a through hole; 7. a glass slide; 8. a heating module; 9. an electron microscope; 10. a gelatin layer; 11. a glycerin layer.
Detailed Description
The following description of the embodiments of the present invention will be made with reference to the accompanying drawings:
as shown in figures 1 and 2, the pollen monitoring device comprises a rotating device, a rotary sample table, a glass slide and a heating module, the rotating device is arranged on a base, a rotating shaft of the rotating device is vertically and upwards arranged, the top of the rotating shaft is connected with a rotating sample table, the rotary sample table is provided with a plurality of grooves (4 in the embodiment, the number of the grooves can be set according to the requirement, and the grooves can be set at equal intervals), the grooves are circular, the bottoms of the grooves are provided with through holes, a glass slide is detachably arranged in the groove, a gelatin layer is arranged on the glass slide, a glycerin layer is arranged on the gelatin layer, the base is also provided with a heating module, the heating end of the heating module is arranged corresponding to the groove of the rotary sample table, so that the slide glass can be heated by the heating end of the heating module when the groove rotates to the upper part of the heating module. During the use, through equipment such as striking sample thief with pollen striking on the slide, the pollen is stuck by the glycerine layer this moment, then rotates rotatory sample platform for the slide in the recess is located heating module top, and heats. After heating, the pollen is sunk into the melted gelatin layer, and after cooling, the fixation of the pollen can be realized. And then rotating the rotary sample table, and observing the pollen sample by using an electron microscope.
The rotating device adopts an index plate. The angle of each rotation can be accurately controlled.
The material of the rotary sample table is one of a cast iron plate, a stainless steel plate or a high-temperature-resistant resin plate. Has enough hardness and is not afraid of high temperature.
The heating module is a far infrared heater, and the heating direction of the heating module is vertical upwards. As is known, the far infrared heater has a limited heating effect on an air medium, so most of heat can directly reach the glass slide, the heating effect is improved, the heat loss is reduced, and the air around the device cannot be greatly heated.
One of the ways of use (as shown in fig. 3):
60m from the surrounding air by means of impact collectors3The/h rate collects gas to impact pollen on the slide (of course, pollen may not be present in the air, and thus on the slide), and the collection cycle should be fixed, for example, one collection time of 3 hours.
The rotating sample table is rotated to the heating module, the glass slide is heated to 90 ℃, the viscous surface on the glass slide is melted at high temperature, so that the particles are sunk into the medium, the position of the particles is fixed and adjusted, and observation and focusing of a later electron microscope are facilitated. The rotation can be manual control also can be through controlgear such as PLC (the user installs additional by oneself) carry out automatic control, this is not the utility model discloses in the protection range. The heating temperature requires the user to obtain the optimal heating module parameters through limited experiments.
After heating, the glass slide after heating of the rotary sample stage reaches an observation position (the position cannot be at the position of the heating module necessarily, and actually, the position for installing the glass slide, the position for using the virtual impactor, the position of the heating module, the observation position and the position for taking out the glass slide should be well divided by a user during use), and at the moment, observation and recording can be carried out by an electron microscope. After the recording is completed, the slide with the pollen sample fixed thereon can be taken out and put into a storage box.
The foregoing embodiments and description have been provided to illustrate the principles and preferred embodiments of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed.

Claims (4)

1. Pollen monitoring devices, its characterized in that: including rotating device, rotatory sample platform, slide glass and heating module, rotating device installs on a base, the vertical upwards setting of rotating device's pivot, the pivot top is connected with rotatory sample platform, rotatory sample bench is provided with a plurality of recesses, the recess is circular and its bottom is provided with the through-hole, detachably places slide glass in the recess, be provided with the gelatin layer on the slide glass, be provided with the glycerine layer on the gelatin layer, still install heating module on the base, the heating terminal of heating module corresponds the recess setting of rotatory sample platform.
2. The pollen monitoring device of claim 1, wherein: the rotating device adopts an index plate.
3. The pollen monitoring device of claim 2, wherein: the material of the rotary sample table is one of a cast iron plate, a stainless steel plate or a high-temperature-resistant resin plate.
4. The pollen monitoring device of claim 3, wherein: the heating module is a far infrared heater, and the heating direction of the heating module is vertical upwards.
CN202020019961.8U 2020-01-06 2020-01-06 Pollen monitoring device Active CN211576876U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202020019961.8U CN211576876U (en) 2020-01-06 2020-01-06 Pollen monitoring device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202020019961.8U CN211576876U (en) 2020-01-06 2020-01-06 Pollen monitoring device

Publications (1)

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CN211576876U true CN211576876U (en) 2020-09-25

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Application Number Title Priority Date Filing Date
CN202020019961.8U Active CN211576876U (en) 2020-01-06 2020-01-06 Pollen monitoring device

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111896430A (en) * 2020-01-06 2020-11-06 上海恺擎智能科技有限公司 Pollen monitoring method and device
CN117907162A (en) * 2024-01-24 2024-04-19 重庆市生态环境科学研究院 Atmospheric pollen on-line detection system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111896430A (en) * 2020-01-06 2020-11-06 上海恺擎智能科技有限公司 Pollen monitoring method and device
CN117907162A (en) * 2024-01-24 2024-04-19 重庆市生态环境科学研究院 Atmospheric pollen on-line detection system

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