CN213708372U - Cell counting plate and stored grain fungus detection device - Google Patents
Cell counting plate and stored grain fungus detection device Download PDFInfo
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- CN213708372U CN213708372U CN202022494986.6U CN202022494986U CN213708372U CN 213708372 U CN213708372 U CN 213708372U CN 202022494986 U CN202022494986 U CN 202022494986U CN 213708372 U CN213708372 U CN 213708372U
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Abstract
An embodiment of the utility model discloses a cell counting board and storage grain fungus detection device, include: the counting plate is provided with a counting area, a sample application area, an overflow groove and a cover glass platform, wherein the counting area is used for observing a sample; the sampling area is used for sampling the sample liquid to the sampling area; the overflow groove is used for discharging redundant sample liquid; the cover glass table is used for placing a cover glass. The utility model discloses to present problem, provide a cell counting board, can predetermine focus, check point etc for the fungi detects and to reach the effect that check-up time is short, and the rate of accuracy is high.
Description
Technical Field
The utility model relates to a detection device, more specifically relates to a cell counting board and a storage grain fungus detection device.
Background
The harm of fungi is a problem of grain storage safety which faces the world together. According to FAO statistics, the grain loss caused by fungal hazards accounts for 25% of the grain yield every year, the grain loss caused by fungal hazards in the storage process accounts for a considerable proportion, the direct economic loss caused every year in the United states is about $ 10 million, and in some regions of China, the local heating and mildew phenomena of grains in the storage process sometimes occur, and the phenomenon is even more serious in some regions, so that the grain safety of China is seriously threatened.
When detecting grain storage fungi, the method is mainly based on a plate colony counting method, is a classic method for food microorganism detection, can be used for detecting most fungi, and is complex to operate and needs to be operated by professionals under a sterile condition. The time is long, and seven days are usually required. The method is used for detecting the fungi harmful to stored grains, and because of the growth characteristics of an important fungus, namely aspergillus glaucus, in the fungi, even if the fungi grow in an optimal high-sugar high-salt culture medium, the fungi grow very slowly, sometimes the fungi are cultured for more than 10 days, spores cannot completely germinate, and the grown hyphae cannot form regular colonies, so that the difficulty is brought to colony counting, and the experimental error is directly increased. Therefore, the method is low in efficiency, and the detection device aiming at the method cannot be applied to quick automatic detection.
The industrial standard of detection of grain and oil inspection and grain storage fungi-spore counting method is promulgated and implemented in 2018 in 3 months, and fills the gap of detection of the grain storage fungi in China. The method needs the tester to identify and count the fungal spores by means of microscope amplification effect and visual observation. Therefore, the detection personnel is required to have a certain microorganism operation basis, and the detection personnel at the grain depot base level generally do not have the professional knowledge of the microorganisms, so that the popularization and the application of the method are limited to a certain extent.
Based on the reasons, an instrumental fungal spore counting device needs to be developed, the rapid automatic detection can be realized while the accuracy is ensured, and the instrumental fungal spore counting device has great significance for the detection of the grain storage fungi.
Disclosure of Invention
An object of the utility model is to provide a cell counting plate.
Another object of the utility model is to provide a store up grain fungus detection device.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model discloses the first aspect provides a cell count board, include: the counting plate is provided with a counting area, a sampling area, an overflow groove and a cover glass platform, wherein
The counting area is used for observing the sample;
the sampling area is used for sampling the sample liquid to the sampling area;
the overflow groove is used for discharging redundant sample liquid;
the cover glass table is used for placing a cover glass.
In one embodiment, a sample application zone is provided at the edge of each counting zone, and a sample solution is applied to the sample application zone and flows into the counting zone by capillary action of the sample solution after the cover slip is placed on the cover slip stage.
In one embodiment, the height of the sample fluid is 0.1mm after the sample fluid enters the counting zone.
In a specific embodiment, the counting area is 0.1mm lower than the cover slip stage.
In a specific embodiment, an overflow groove is arranged around the counting area, and the overflow groove is 0.2mm lower than the cover glass stage.
In one specific embodiment, a focusing line is arranged in the center of the counting area, and the focusing line is a cross line consisting of 0.01mm thick line sections and 0.06mm long line sections.
In one embodiment, the focusing lines are 10 rows and 10 columns in total, each row and each column being spaced 0.1mm apart, forming a black focusing area.
The utility model discloses the second aspect provides a store up grain fungus detection device, including above-mentioned counting plate, injector, three-dimensional automatic displacement focus subassembly, enlarge light path subassembly and shoot the subassembly, wherein
The counting plate is used for manufacturing a sample wafer;
the sample injector is used for fixing the counting plate on the sample table, the corresponding position of each counting area is marked with a counting area number, and the edge of the sample injector is fixed in the center of the movable sample table through a dovetail slot type design;
the three-dimensional automatic displacement focusing assembly is used for moving the counting plate in position and automatically focusing the counting plate to the center of the visual field of the shooting assembly;
the amplifying light path component is used for amplifying the image of the counting area in the sample wafer;
the shooting component is used for collecting the image of the counting area in the sample wafer and sending the collected image to the computer.
In one embodiment, the three-dimensional automatic displacement focusing assembly comprises: a stepping motor, a coupling, a screw, a ball nut base, a screw holder and a mobile station, wherein
The stepping motor is connected with the screw rod through the coupler, the screw rod can be driven to rotate by rotation of the stepping motor, the mobile platform is connected with the screw rod through the ball nut base, and when the screw rod rotates, the ball nut base converts rotation of the screw rod into linear motion, so that linear motion of the mobile platform is achieved.
In one embodiment, the magnifying optical path assembly comprises a 40-fold objective lens, an infinite independent achromatic system and a 0.5-inch 500-thousand-pixel industrial camera, and the actual shooting range of the optical path is 0.2mm x 0.3 mm.
The utility model has the advantages as follows:
1. the focus, the detection points and the like are preset through software, and the chip controls the stepping motor to automatically finish the moving shooting of all preset points, so that the automatic detection of the instrument is realized.
2. The utility model discloses a microscopic auto focus function is realized to the image definition algorithm, reduces the personnel's operation degree of difficulty, the utility model discloses check-out time is short, and the rate of accuracy is high, has realized the automatic intellectual detection system of storage grain fungus.
Drawings
The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.
Fig. 1 shows a schematic composition diagram of the counting plate in the present embodiment.
Fig. 2 shows a schematic diagram of a black focus region in the present embodiment.
Fig. 3 shows a schematic composition diagram of the detection device for grain storage fungi in this embodiment.
Fig. 4 shows a schematic diagram of the sample injector and sample stage in this example.
Detailed Description
In order to explain the present invention more clearly, the present invention will be further described with reference to the preferred embodiments and the accompanying drawings. Similar parts in the figures are denoted by the same reference numerals. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the invention.
Example one
As shown in figure 1, the utility model discloses a cell counting plate, the counting plate is made by glass, is provided with count region 4, point appearance region 2, overflow launder 5 and cover glass platform 1 on the counting plate, wherein
The counting area 4 is used for observing the sample;
the sample application area 2 is used for applying sample liquid to the sample application area;
the overflow groove 5 is used for discharging redundant sample liquid;
the cover glass stage 1 is used for placing a cover glass.
In one embodiment, the edge of each counting area 4 is provided with a spot area 2, and when the cover glass is placed on the cover glass stage 1, a spot of the sample liquid is applied to the spot area 2 and flows into the counting area by capillary action of the sample liquid.
In one embodiment, the height of the sample fluid is 0.1mm after the sample fluid enters the counting area 4.
In a specific embodiment, the counting area 4 is 0.1mm lower than the cover glass stage 1.
In a specific embodiment, the H-shaped area around the upper and lower counting areas is provided with an overflow groove 5, and the overflow groove 5 is 0.2mm lower than the cover glass table 1.
In a specific embodiment, for example, every counting board sets up 6 count districts, can be used to observe 6 samples, 6 point appearance district, 6 black focusing areas, and every upper and lower two count district H type region all around is provided with the overflow launder, the utility model discloses it is only exemplary to set up 6 count districts to every counting board, the utility model discloses do not do the restriction to the number that every counting board set up count district, can set up according to the demand by oneself.
In one specific embodiment, a focusing line is arranged in the center of the counting area, and the focusing line is a cross line consisting of 0.01mm thick line sections and 0.06mm long line sections.
In one embodiment, the focusing lines are 10 rows and 10 columns in total, each row and each column being spaced 0.1mm apart, forming a black focusing area.
In a specific embodiment, every count district middle part is provided with the focus district for focus the subassembly and find the position of focusing, for black in this embodiment to focus the schematic diagram in district as shown in fig. 2, it is used for the detection of shooing to predetermine 16 points around the district of focusing, so 16 photos need to be shot to every sample, 16 count result sums of spore image multiply the result of relevant dilution multiple again, be the fungal spore quantity of this sample promptly, the utility model discloses it is only exemplary to shoot 16 photos to every sample setting, the utility model discloses the number of shooting the photo is not restricted to every sample setting, can set up according to the demand by oneself.
The utility model discloses to present problem, provide a cell counting board, can predetermine focus, check point etc for the fungi detects and to reach the effect that check-up time is short, and the rate of accuracy is high.
Example two
As shown in FIG. 3, the utility model discloses a store up grain fungus detection device, detection device includes counter plate, injector, three-dimensional auto-displacement subassembly of focusing, enlargies light path subassembly and shoots the subassembly, wherein
The counting plate is used for manufacturing a sample wafer;
the sample injector is used for fixing the counting plate on the sample table, the corresponding position of each counting area is marked with a counting area number, and the edge of the sample injector is fixed in the center of the counting plate through a dovetail slot type design;
the three-dimensional automatic displacement focusing assembly is used for moving the counting plate in position and automatically focusing the counting plate to the center of the visual field of the shooting assembly;
the amplifying light path component is used for amplifying the image of the counting area in the sample wafer;
the shooting component is used for collecting the image of the counting area in the sample wafer and sending the collected image to the computer.
In a specific embodiment, after preparation of a fungal spore sample liquid, a cover glass is placed on a cover glass table of a counting plate, the sample liquid is transferred through a dropper, the sample liquid is spotted to a sample spotting area, the sample liquid flows into the counting area through capillary action after contacting the edge of the cover glass, redundant sample liquid is led out through an overflow groove, the counting plate is fixed on a sample injector, the sample preparation is completed, the sample injector is used for fixing the counting plate and the sample table, the sample injector is marked with numbers of 1-6 at the corresponding position of the counting plate and used for marking the counting area, as shown in figure 4, the sample injector and the sample table are schematically shown, the sample injector is inserted from a sample inlet of a front panel of the instrument and is fixed through a clamping groove after being inserted to the bottom, the sample injector is reserved for 1cm outside the instrument, and is conveniently taken out after detection is. It should be noted that, the number of the serial numbers of the sample injector set at the corresponding position of the counting plate is only exemplary, and the present invention does not limit this.
In one embodiment, the three-dimensional automatic displacement focusing assembly comprises: a stepping motor, a coupling, a screw, a ball nut base, a screw holder and a mobile station, wherein
After a detection program of the detection device starts, the software sends a detection instruction and a moving coordinate to the hardware moving control chip, the chip drives the stepping motor to rotate, the stepping motor is connected with the lead screw through the coupler, the lead screw can be driven to rotate by the rotation of the stepping motor, the moving platform is connected with the lead screw through the ball nut base, and when the lead screw rotates, the ball nut base converts the rotation of the lead screw into linear motion, so that the linear motion of the moving platform is achieved.
And moving the mobile platform to enable the focus of the counting area to be placed under the visual field of the shooting assembly, calculating the image definition through a brenner image algorithm, adjusting the focal length, moving the counting area to the visual field by the mobile platform after the focal length is adjusted, further adjusting the focal length by the shooting assembly, shooting the spot, and sending the spore image to software for identification.
In one embodiment, the magnifying optical path assembly comprises a 40-fold objective lens, an infinite independent achromatic system and a 0.5-inch 500-ten-thousand-pixel industrial camera, the optical path takes an actual shot range of 0.2mm 0.3mm, each counting area takes 16 photographs due to the actual shot range of 0.2mm 0.3mm of each photograph, and the total shot range is 0.2mm 0.3mm 16 mm 0.96mm2Since the height of the sample solution is 0.1mm, the total volume of the photographed sample solution is 0.1mm3And calculating the actual spore content of the sample by calculating the dilution times of the sample solution and the mass of the sample.
In one embodiment, the back of the detection device is provided with a power socket and a data transmission socket, and the data transmission socket is connected with a computer.
The utility model provides a grain storage fungus detection device, which can automatically complete the moving shooting of all preset points by a chip control stepping motor through presetting a focus point, a detection point and the like by software, thereby realizing the automatic detection of an instrument; the utility model discloses a microscopic auto focus function is realized to the image definition algorithm, reduces the personnel's operation degree of difficulty, the utility model discloses check-out time is short, and the rate of accuracy is high, has realized the automatic intellectual detection system of storage grain fungus.
Obviously, the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it is obvious for those skilled in the art to make other variations or changes based on the above descriptions, and all the embodiments cannot be exhausted here, and all the obvious variations or changes that belong to the technical solutions of the present invention are still in the protection scope of the present invention.
Claims (10)
1. A cell counting plate, comprising: the counting plate is provided with a counting area, a sampling area, an overflow groove and a cover glass platform, wherein
The counting area is used for observing the sample;
the sampling area is used for sampling the sample liquid to the sampling area;
the overflow groove is used for discharging redundant sample liquid;
the cover glass table is used for placing a cover glass.
2. The counting plate of claim 1, wherein each counting area has a spot area at an edge thereof, and wherein a spot of sample fluid is applied to the spot area and flows into the counting area by capillary action of the sample fluid when the cover slip is placed on the cover slip platform.
3. The counting plate of claim 1, wherein the height of the sample liquid is 0.1mm after the sample liquid enters the counting area.
4. The count plate of claim 1 wherein said count zone is 0.1mm below the cover glass stage.
5. The count plate of claim 1 wherein an overflow well is provided around said counting area, said overflow well being 0.2mm below the cover glass stage.
6. The counting plate of claim 1, wherein the counting area is centrally provided with a focusing line which is a cross line consisting of 0.01mm thick and 0.06mm long sections.
7. The counting plate of claim 6, wherein said focusing lines are 10 rows and 10 columns in total, each row being spaced 0.1mm apart, forming a black focusing area.
8. A grain storage fungus detection device, which is characterized by comprising the counting plate, a sample injector, a three-dimensional automatic displacement focusing assembly, an amplifying light path assembly and a shooting assembly of any one of claims 1 to 7, wherein the counting plate, the sample injector, the three-dimensional automatic displacement focusing assembly, the amplifying light path assembly and the shooting assembly are arranged in sequence, and the grain storage fungus detection device is characterized in that the counting plate
The counting plate is used for manufacturing a sample wafer;
the sample injector is used for fixing the counting plate on the sample table, the corresponding position of each counting area is marked with a counting area number, and the edge of the sample injector is fixed in the center of the movable sample table through a dovetail slot type design;
the three-dimensional automatic displacement focusing assembly is used for moving the counting plate in position and automatically focusing the counting plate to the center of the visual field of the shooting assembly;
the amplifying light path component is used for amplifying the image of the counting area in the sample wafer;
the shooting component is used for collecting the image of the counting area in the sample wafer and sending the collected image to the computer.
9. The apparatus of claim 8, wherein the three-dimensional auto-displacement focusing assembly comprises: a stepping motor, a coupling, a screw, a ball nut base, a screw holder and a mobile station, wherein
The stepping motor is connected with the screw rod through the coupler, the screw rod can be driven to rotate by rotation of the stepping motor, the mobile platform is connected with the screw rod through the ball nut base, and when the screw rod rotates, the ball nut base converts rotation of the screw rod into linear motion, so that linear motion of the mobile platform is achieved.
10. The apparatus of claim 8, wherein the magnifying beam path assembly comprises a 40 x objective, an infinity independent achromatic system, and a 0.5 inch 500 ten thousand pixel industrial camera, and the beam path captures an actual capture range of 0.2mm x 0.3 mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022494986.6U CN213708372U (en) | 2020-11-02 | 2020-11-02 | Cell counting plate and stored grain fungus detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022494986.6U CN213708372U (en) | 2020-11-02 | 2020-11-02 | Cell counting plate and stored grain fungus detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023133714A1 (en) * | 2022-01-12 | 2023-07-20 | 广州工商学院 | Fungus quantity measurement device and measurement method for testing yogurt quality |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023133714A1 (en) * | 2022-01-12 | 2023-07-20 | 广州工商学院 | Fungus quantity measurement device and measurement method for testing yogurt quality |
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