CN216748268U - Microscopic imaging device with optimized focusing operation - Google Patents
Microscopic imaging device with optimized focusing operation Download PDFInfo
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- CN216748268U CN216748268U CN202122738879.8U CN202122738879U CN216748268U CN 216748268 U CN216748268 U CN 216748268U CN 202122738879 U CN202122738879 U CN 202122738879U CN 216748268 U CN216748268 U CN 216748268U
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Abstract
The utility model relates to a microscopic imaging device for optimizing focusing operation, which comprises a main control assembly, wherein a first driving part, a second driving part, a temperature control type objective table, a bilateral telecentric light path structural part and a light source which are connected with the main control assembly are also arranged on the device; the first driving part is connected with the temperature control type objective table to control the horizontal movement of the objective table, the second driving part is connected with the bilateral telecentric optical path structural part to control the vertical movement of the objective table, the object side of the bottom end of the bilateral telecentric optical path structural part corresponds to the upper part of the temperature control type objective table, and the image side of the top end of the bilateral telecentric optical path structural part is provided with an image sensor; the light emitted from the light source penetrates through a sample to be observed and detected and is incident to the bilateral telecentric light path structural part. The device adopts a bilateral telecentric light path structural member, has the characteristics of large depth of field and stable imaging quality, optimizes the focusing process of the conventional microscope device, can reduce the construction cost, and realizes the automation and rapid detection of biological samples.
Description
Technical Field
The utility model relates to the field of biological sample detection, in particular to a microscopic imaging device.
Background
In the field of biological sample analysis, such as semen quality analysis, blood cell counting, etc., it is becoming more common to acquire a series of sample images by using a microscopic imaging device, and then to analyze the biological sample with the assistance of a computer in a main control assembly.
In practical applications, a digital microscope is generally used to observe and analyze biological samples. However, the digital microscope is expensive, has high professional literacy requirement and low working efficiency during operation, is not beneficial to large-scale application of small and medium-sized production enterprises, and is particularly insufficient in the traditional microscope image analysis method for analyzing scenes with high real-time analysis requirements, such as rapid detection and subpackage of fresh pig sperms. The reason is that after the biological counting plate is switched every time, an operator needs to combine and adjust the coarse focusing screw and the fine focusing screw to perform focusing operation, so that the analyzed sample image is clearly imaged.
In order to solve the pain, many manufacturers or researchers have proposed an automatic focusing method based on image definition evaluation. According to the method, manual focusing is converted into automatic focusing, manual operation is reduced, production efficiency is improved to a certain extent, but due to the introduction of an automatic focusing system, on one hand, product cost can be correspondingly improved, and on the other hand, system reliability of products can be reduced.
In summary, there is still room for improvement in existing microscopic imaging apparatuses.
SUMMERY OF THE UTILITY MODEL
One technical problem addressed by one aspect of the present disclosure is to provide an improved microscopic imaging apparatus.
The technical scheme adopted by the utility model for solving the technical problems is as follows: the microscopic imaging device with optimized focusing operation comprises a main control assembly, and a first driving part, a second driving part, a temperature control type objective table, a bilateral telecentric light path structural part and a light source which are connected with the main control assembly are further arranged on the device; the first driving part is connected with the temperature-controlled objective table to control the horizontal movement of the objective table, the temperature-controlled objective table comprises an object placing part for placing a sample to be observed and detected and a temperature control assembly for adjusting the temperature of the object placing part, and the object placing part is provided with a light transmission position; the second driving part is connected with the bilateral telecentric optical path structural part to control the bilateral telecentric optical path structural part to vertically move, the object side at the bottom end of the bilateral telecentric optical path structural part corresponds to the upper part of the temperature control type objective table and is used for shooting a sample to be observed, and the image side at the top end of the bilateral telecentric optical path structural part is provided with an image sensor to transmit image information; and the light-emitting path of the light source passes through the light-transmitting position to penetrate through a to-be-observed detection sample to be incident to the bilateral telecentric light path structural part.
The microscopic imaging apparatus optimized for focusing operation as described above, the object side is provided with a fixed focus auxiliary ring for focusing,
the microscopic imaging device with optimized focusing operation comprises a temperature control component, a temperature control PCB circuit board and a metal heat conducting plate, wherein the temperature control component is connected with the temperature control PCB circuit board and the metal heat conducting plate, and the metal heat conducting plate is provided with a groove to form the object placing part.
In the microscopic imaging device optimized in focusing operation, the bottom of the groove is provided with a through channel to form the light transmission position; the light source is positioned below the light-transmitting position.
In the microscopic imaging device optimized in focusing operation, the two sides of the placing part are provided with the slots so as to take and place the detection sample to be observed.
In the microscopic imaging device with optimized focusing operation, the first driving part comprises a first stepping motor, and the transmission output end of the first stepping motor is connected with the metal heat-conducting plate to control the horizontal movement of the first stepping motor.
In the microscopic imaging device optimized in focusing operation, the second driving part includes a second stepping motor, a transmission output end of the second stepping motor is connected to a fixing seat, the fixing seat has an assembly hole, the bilateral telecentric optical path structural member is installed in the assembly hole, and has a preset longitudinal stroke compared with the assembly hole to realize the up-and-down movement under corresponding stress; and a buffer spring is arranged between the fixed seat and the fixed focus auxiliary ring.
In the microscopic imaging device optimized in focusing operation, the fixed-focus auxiliary ring is assembled with the bilateral telecentric optical path structural member by adopting a threaded structure.
One advantageous effect brought by one aspect of the present disclosure: the microscopic imaging device adopts a bilateral telecentric light path structural member, has the characteristics of large depth of field and stable imaging quality, optimizes the focusing process of the conventional microscopic device, can reduce the construction cost, realizes the automatic and rapid detection of biological samples, and has wide application prospect in the fields of semen analysis, urine analysis, cell analysis and the like.
Drawings
Certain embodiments of the utility model will now be described in detail, by way of example and not limitation, with reference to the figures, wherein like reference numerals identify identical or similar elements or portions. Those skilled in the art will appreciate that the drawings are not necessarily drawn to scale.
In the drawings:
FIG. 1 is a schematic view of a first aspect of the present invention;
FIG. 2 is a schematic view from a second perspective of the present invention;
FIG. 3 is a schematic view of a portion of a temperature controlled stage according to the present invention;
the designations in the figures illustrate the following:
1. a frame; 2. a temperature controlled stage; 200. an article placing part; 201. a first driving section; 202. a light transmitting position; 203. a slot position; 3. a bilateral telecentric optical path structure; 300. a fixed focus auxiliary ring; 301. an image sensor; 302. a second driving section; 303. a fixed seat; 304. a buffer spring; 4. a light source.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the drawings of the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments.
All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the utility model without any inventive step, are within the scope of protection of the utility model. Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs.
The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. Also, the use of the terms "a," "an," or "the" and similar referents do not denote a limitation of quantity, but rather denote the presence of at least one. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
Referring to fig. 1-3, a microscope imaging apparatus optimized for focusing operation is shown, the microscope imaging apparatus generally uses a frame 1 as a carrier to mount other corresponding structures, a main control assembly thereof generally includes a computer system, such as a common single chip system, an FPGA system, an android system, a Windows system, and the like, and data in the apparatus can be analyzed and processed through the system, and the corresponding structures can be operated and controlled. The device is also provided with a first driving part 201, a second driving part 302, a temperature control type objective table 2, a double-side telecentric light path structural part 3 and a light source 4 which are connected with the main control assembly; the first driving part 201 is connected with the temperature-controlled object stage 2 to control the horizontal movement of the object stage, the temperature-controlled object stage 2 comprises an object placing part 200 for placing a sample to be observed and a temperature control component for adjusting the temperature of the object placing part 200, and the object placing part 200 is provided with a light transmitting position 202; the second driving part 302 is connected with the double-sided telecentric optical path structural part 3 to control the vertical movement of the structural part, the object side at the bottom end of the double-sided telecentric optical path structural part 3 corresponds to the upper part of the temperature-controlled object stage 2 and is used for shooting a sample to be observed, and the image side at the top end of the double-sided telecentric optical path structural part 3 is provided with an image sensor 301 for transmitting image information; the light-emitting path of the light source 4 passes through the light-transmitting position 202 to penetrate through the sample to be observed and enter the double-sided telecentric light path structural member 3.
A counting plate is generally used as a carrier for biological samples to be observed and detected, the counting plate is placed on the object placing part 200, the temperature control assembly provides a stable and constant temperature environment suitable for detection for the biological samples by adjusting the temperature, light rays irradiate the biological samples on the counting plate through the light transmitting position 202 after the light source 4 is started, transmitted light vertically irradiated by the light source 4 is amplified through the bilateral telecentric light path structural part 3 and is subjected to induction imaging on the image sensor 301, the obtained images are transmitted to the computer system of the main control assembly, and the obtained images can be directly displayed and observed on a display or are analyzed through an algorithm to obtain related information of the biological samples.
The first driving unit 201 drives the temperature-controlled stage 2 (loaded cell counting plate) to move horizontally, and can provide a plurality of views for the image sensor 301. When the observation field of view needs to be switched, the second driving portion 302 drives the double-sided telecentric optical path structural member 3 to vertically move upwards until the lower end face of the double-sided telecentric optical path structural member 3 is separated from the upper end face of the cell counting plate by a distance and then stops (returns to the initial position). At this time, the first driving portion 201 controls the temperature-controlled object stage 2 to move horizontally, so as to switch the observation field of view, and after the observation field of view is switched, the second driving portion 302 drives the bilateral telecentric optical path structural member 3 to vertically move downwards (reach the detection position), thereby ensuring that the lower end surface of the bilateral telecentric optical path structural member 3 is in close contact with the upper end surface of the cell counting plate.
As is well known, as the field of view to be observed is continuously reduced, the corresponding optical magnification is gradually increased, the depth of field of the optical system is inevitably reduced, and the smaller the depth of field is, the higher the focusing requirement of the optical imaging system is, or the higher the precision requirement of the relative position is. For the traditional biological microscope, because the depth of field is very small (under 10 times of objective lens, the depth of field is about 8um), the precision requirement of the imaging carrier (objective table or counting plate) is beyond the conventional requirement, and the realization of the product is not facilitated.
The scheme adopts a (object image) double-sided telecentric optical path structural component 3, firstly confirms the magnification of the double-sided telecentric optical path structural component 3, the visual field range of a biological sample image and the size design requirement of a microscopic imaging device, and confirms the thickness processing error d of a cell counting plate1Calculating the depth of field d of the optical path system2Continuously adjusting the design to ensure that condition d is satisfied2>d1And selecting an optical device with a proper specification to build the double-side telecentric optical path structural member 3 and packaging the double-side telecentric optical path structural member into a module so as to be assembled and fixed with the image sensor 301 and the external fixed-focus auxiliary ring 300.
The bilateral telecentric optical path structural component 3 has the advantages of both an object-side telecentric optical path and an image-side telecentric optical path, and the magnification factor is not influenced no matter how far and near an object is or how far and near a camera is, so that when the biological sample moves back and forth on a focal plane, the magnification factor of a target is consistent, and repeated calibration in the imaging measurement process is avoided. Another outstanding advantage is large depth of field, for 0.5mm2-0.8mm2The field depth of field of the counting plate can reach 60um-120um, which is 3-6 times of that of the traditional optical microscope, and when the height error of the counting plate is controlled within the field depth range, the sample can be imaged clearly even after the counting plate is replaced. Therefore, the distance between the biological sample and the bilateral telecentric optical path structural member 3 can be controlled through the thickness and the size of the counting plate in the scheme, and the counting plate can still clearly image without focusing.
Further, the object side is provided with a fixed focus auxiliary ring 300 for focusing, that is, the distance between the lower end surface of the fixed focus auxiliary ring 300 and the object side end surface of the double-sided telecentric optical path structure 3 can be dynamically adjusted, for example, both are adjusted by using a screw structure. For different biological sample analysis scenes, the thickness of the cell counting plate is generally millimeter-scale difference, and the depth of field range of the imaging device is in micrometer scale, so that initial focusing is preferably performed when different types of cell counting plates are replaced. And adjusting the distance between the bilateral telecentric optical path structural member 3 and the cell counting plate by virtue of the fixed-focus auxiliary ring 300 to finish initial focusing. For products in different detection fields, initial focusing work is only required to be finished before delivery, and customers in different fields can directly use the products without focusing again.
Specifically, after the design of the microscopic imaging device is completed, the distance S between the target and the bilateral telecentric optical path structural member 3 is determined to be unchanged, corresponding fixed-focus initial distance L is designed in advance for different types of cell counting plates a and B or more types of cell counting plates, the thickness of a cover glass of the cell counting plate and the thickness of a sample layer are defined as X, and the relation L + X is satisfied. When the cell counting plates of different types need to be replaced, the fixed-focus auxiliary ring 300 is adjusted to a corresponding appointed position according to the types of the cell counting plates, initial focusing is completed, the cell counting plates of the same type are replaced, and the fixed-focus auxiliary ring 300 does not need to be adjusted. When the lower end face of the fixed-focus auxiliary ring 300 is in close contact with the upper end face of the cell counting plate, the biological sample target is just in the depth of field range of the bilateral telecentric optical path structural member 3, and the image sensor 301 can directly receive and store the clear biological sample target image, so that the focusing-free microscopic imaging is realized, the imaging speed is increased, and the cost is greatly reduced.
In some embodiments, the temperature control assembly includes a temperature control PCB and a metal heat conducting plate connected to each other, the metal heat conducting plate is provided with a groove to form the object placing portion 200, and the bottom of the groove is provided with a through channel to form the light transmitting portion 202; the light source 4 is located below the light-transmitting position 202. The general temperature control PCB circuit board has the heating function and the temperature measuring function, when the temperature is lower than the set threshold temperature (the temperature environment required by the biological sample is generally 37 ℃), the circuit board starts to be heated, and when the temperature is higher than or equal to the threshold temperature, the heating is stopped. The metal heat-conducting plate is in close contact with the temperature control PCB circuit board, and the heat of the temperature control PCB circuit board is transferred to the cell counting plate through the metal heat-conducting plate, so that a stable and suitable constant temperature environment for detection is provided for the biological sample.
In some embodiments, the bottom of the groove is provided with a through channel to form the light-transmitting position 202; the light source 4 is located below the light-transmitting position 202. The light source 4 can be installed on the frame 1 through fasteners such as bolts and the like, and is positioned right below the channel, and after the light source is started, the light rays vertically irradiate the counting plate of the object placing part 200.
In some embodiments, two sides of the placement part 200 are provided with slots 203 for taking and placing a test sample to be observed. The trench 203 on both sides is generally symmetrical setting, makes things convenient for the user to point to exert oneself and get the counting board.
In some embodiments, the first driving part 201 includes a first stepping motor, and a transmission output end of the first stepping motor is connected to the metal heat-conducting plate to control horizontal movement thereof. The linear motion of the corresponding structure is controlled by adopting a linear stepping component, the first stepping motor is fastened on a frame 1 of the microscopic imaging device, and the metal heat conducting plate is connected with a transmission output end of the first stepping motor, so that the first stepping motor is controlled by the metal heat conducting plate to move forwards and backwards so as to adjust the image visual field.
In some embodiments, the second driving portion 302 includes a second stepping motor, a transmission output end of the second stepping motor is connected to a fixing base 303, the fixing base 303 has a mounting hole, the double-sided telecentric optical path structural component 3 is mounted in the mounting hole, and has a preset longitudinal stroke compared with the mounting hole to realize the up-and-down movement under corresponding stress; a buffer spring 304 is disposed between the fixed seat 303 and the fixed focus auxiliary ring 300.
Specifically, the inner diameter of the assembly hole is slightly larger than the outer diameter of the double-side telecentric optical path structural member 3, the preset longitudinal stroke of the assembly hole and the double-side telecentric optical path structural member can be controlled by matching a common longitudinal stroke groove and a stroke stop block, and can also be controlled by an image sensor 301 at the top end of the double-side telecentric optical path structural member 3 and a focusing auxiliary ring 300 at the bottom end of the double-side telecentric optical path structural member, so that the double-side telecentric optical path structural member 3 can move up and down along the assembly hole for a certain stroke, and when the double-side telecentric optical path structural member moves to the end of the stroke, the stop block or the image sensor 301 and the focusing auxiliary ring 300 can prevent the double-side telecentric optical path structural member 3 from separating from the hole upwards or downwards.
The arrangement of the buffer spring 304 (compression spring) enables the bilateral telecentric optical path structural component 3 to be at the bottom end of the longitudinal stroke in the initial state, the transmission distance of the second stepping motor is slightly larger than the distance between the lower end face of the fixed-focus auxiliary ring 300 and the upper end face of the cell counting plate in the initial state in practical application, and the existence of the buffer spring 304 provides a possible buffer action for the fixed-focus auxiliary ring 300, so that the transmission distance of the stepping motor does not need to be accurately calculated, and the precision requirement on the stepping motor can be reduced.
In conclusion, the microscopic imaging device can timely adjust the observation field of view and the distance between the lens and the sample through the matching of the first driving part and the second driving part, the adopted double-side telecentric optical path lens obtains a larger field depth range, and the biological sample can be ensured to be in the field depth range of the clear imaging through counting plates with different thicknesses under the condition of no focusing structure. Of course, the device preferably employs a fixed focus auxiliary ring, thereby being flexibly adaptable to different thickness meter plates for detecting different types of biological samples. From this, microscopic imaging device in this application has big depth of field, imaging quality stable characteristics, can realize exempting from to focus or simplify focusing operation, realizes automation and short-term test to biological sample.
For scenes needing multi-view synchronous observation and analysis, the microscopic imaging device can be directly expanded and copied and placed according to a certain rule (unidirectional or array), and multi-view direct observation or synchronous analysis can be realized. For scenes with multi-view observation and low synchronization requirements, a single set of microscopic imaging device can be observed movably according to a certain rule (unidirectional or array), namely, the multi-view observation or analysis can be realized by moving the imaging device or the cell counting plate on an XY coordinate plane.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, as it will be apparent to those skilled in the art that various modifications, combinations and variations can be made in the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.
Claims (8)
1. Microscopic imaging device that focusing operation was optimized, including the master control subassembly, its characterized in that: the device is also provided with a first driving part, a second driving part, a temperature control type objective table, a bilateral telecentric light path structural part and a light source which are connected with the main control assembly;
the first driving part is connected with the temperature-controlled objective table to control the horizontal movement of the objective table, the temperature-controlled objective table comprises an object placing part for placing a sample to be observed and detected and a temperature control assembly for adjusting the temperature of the object placing part, and the object placing part is provided with a light transmission position;
the second driving part is connected with the bilateral telecentric optical path structural part to control the bilateral telecentric optical path structural part to vertically move, the object side at the bottom end of the bilateral telecentric optical path structural part corresponds to the upper part of the temperature control type objective table and is used for shooting a sample to be observed, and the image side at the top end of the bilateral telecentric optical path structural part is provided with an image sensor to transmit image information;
and the light-emitting path of the light source passes through the light-transmitting position to penetrate through a to-be-observed detection sample to be incident to the bilateral telecentric light path structural part.
2. A microscopic imaging apparatus optimized for focusing operations according to claim 1, wherein:
the object side is provided with a fixed focus auxiliary ring for focusing.
3. A microscopic imaging apparatus optimized for focusing operations as set forth in claim 2, wherein:
the temperature control assembly comprises a temperature control PCB and a metal heat-conducting plate which are connected with each other, and a groove is formed in the metal heat-conducting plate to form the object placing part.
4. A microscopic imaging apparatus optimized for focusing operations according to claim 3, wherein:
the bottom of the groove is provided with a through channel to form the light-transmitting position; the light source is positioned below the light-transmitting position.
5. A microscopic imaging apparatus optimized for focusing operations according to claim 3, wherein:
the two sides of the object placing part are provided with slot positions so as to conveniently take and place a sample to be observed and detected.
6. A microscopic imaging apparatus optimized for focusing operations according to claim 3, wherein:
the first driving part comprises a first stepping motor, and the transmission output end of the first stepping motor is connected with the metal heat-conducting plate to control the horizontal movement of the metal heat-conducting plate.
7. A microscopic imaging apparatus optimized for focusing operations as set forth in claim 2, wherein:
the second driving part comprises a second stepping motor, the transmission output end of the second stepping motor is connected with a fixed seat, the fixed seat is provided with an assembly hole, the bilateral telecentric optical path structural part is installed in the assembly hole, and the bilateral telecentric optical path structural part has a preset longitudinal stroke compared with the assembly hole so as to realize the up-and-down motion when correspondingly stressed; and a buffer spring is arranged between the fixed seat and the fixed focus auxiliary ring.
8. A microscopic imaging apparatus optimized for focusing operations as set forth in claim 2, wherein:
the fixed focus auxiliary ring is assembled with the bilateral telecentric optical path structural part by adopting a threaded structure.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115144404A (en) * | 2022-09-01 | 2022-10-04 | 茉丽特科技（深圳）有限公司 | Vision measuring instrument based on telecentric optics technology |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115144404A (en) * | 2022-09-01 | 2022-10-04 | 茉丽特科技（深圳）有限公司 | Vision measuring instrument based on telecentric optics technology |
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