CN102305791B - Slide specimen image acquiring method - Google Patents
Slide specimen image acquiring method Download PDFInfo
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- CN102305791B CN102305791B CN2011102010625A CN201110201062A CN102305791B CN 102305791 B CN102305791 B CN 102305791B CN 2011102010625 A CN2011102010625 A CN 2011102010625A CN 201110201062 A CN201110201062 A CN 201110201062A CN 102305791 B CN102305791 B CN 102305791B
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Abstract
The invention discloses a slide specimen image acquiring method which relates to a method for acquiring a photograph of a slide specimen by a biological microscope. The method is used for solving the problem that a microscope can obtain a clear image which meets the requirement under a high-power lens under the condition that the thickness of the slide specimen is uneven. The method comprises the following steps of: presetting a slide specimen attribute for selecting; and presetting the focusing grade of the biological microscope for executing image acquisition under the high-power lens, wherein the focusing grade comprises four grades. After an objective lens is roughly adjusted under the high-power lens, the system automatically executes image acquisition work according to the corresponding relation of the attribute of the slide specimen selected by a user, the attribute of the preset slide specimen and the focusing grade, a low-power lens vision image and the corresponding focusing grade.
Description
Technical field
The present invention relates to a kind of biology microscope lens device that is used for, particularly gather the method for the photo of slide sample with biological microscope.
Background technology
Along with automaticity constantly develops, increasing full-automatic biological microscope platform is used for Clinical detection, teaching, scientific research.Full-automatic biological microscope platform can move automatically, cooperates the microscope acquisition system, and sample image that can acquisition testing is used for clinical analysis.This type research focuses mostly on and cuts into slices in the animals and plants sample; Tissue sections etc., these samples have a topmost characteristic just to be to use observation under the low power lens, the thickness high conformity of section simultaneously; Automatically behind this collection of bulk sample and the automatic focusing, need not artificial focusing in the process and can obtain picture rich in detail.But observation dyeing blood smear has very big difference with it, the first, and sample need observe under high power lens usually that (high power lens: oily mirror), error of focusing causes picture to blur; The second, sample area is big, and the thickness consistance is bad, and the whole one-tenth of thickness graded causes figure fuzzy.The focusing bearing calibration of the blood smear of observation dyeing at present can't be satisfied the observation of blood sample.
Summary of the invention
The technical matters that the present invention will solve provides a kind of method of slide sample IMAQ, is used to solve under the situation of slide sample became uneven, and microscope can obtain satisfactory picture rich in detail under high power lens.
For addressing the above problem, the present invention proposes a kind of method of slide sample IMAQ, comprising:
Import the attribute of slide sample in advance to the system of biological microscope: the kind and the quality category of slide sample are set in advance, and this classification supplies user to operate selection;
In advance to system's input focusing grade: the biomicroscope focusing grade that carries out image is gathered under high power lens is set in advance; The focusing grade comprises four grades: based on next width of cloth field-of-view image focusing of low power lens once; Focusing is a cell focusing to relative center, the visual field, again each cell in the visual field is carried out the collection of high power lens hypograph; With next width of cloth visual field separated into two parts of low power lens, these two parts all to be focused once, each focusing all is cell focusing placed in the middle relatively in the visual field, one's respective area, after every parts of images focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph; Next width of cloth visual field of low power lens is divided into three parts, each parts of images is all focused once, each focusing all is cell focusing placed in the middle relatively in the visual field, one's respective area, after each part focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph; Next width of cloth visual field of low power lens is divided into four parts, each parts of images is all focused once, each focusing all is cell focusing placed in the middle relatively in the visual field, one's respective area, after each part focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph;
The attribute of slide sample and the corresponding relation of focusing grade are set in advance; The attribute of section has determined the focusing grade.
The quantity of the cell image that needs collection is set in advance;
The user carries out the attribute selection of slide sample according to the attribute of slide sample;
Under the low power objective of biological microscope, slide sample is carried out the low power IMAQ, form the low-power field image;
Object lens turn to rough focusing under the high power lens;
Object lens are under high power lens after the coarse adjustment, and the attribute and the corresponding relation of the attribute that slide sample is set in advance with the focusing grade of the slide sample that system is selected according to the user are accordinged to the low-power field image automatically according to the focusing grade carries out image collecting work of correspondence;
The quantity of the image of the cell of gathering promptly stops IMAQ after reaching the quantity of cell image of the needs collection that is provided with in advance.
Relative center, the visual field is meant the observation center of a cell of the selection in central area, the visual field as the visual field.This center is to confirm with the cell position of central area, and it is not fixed the absolute position, is a relative position, so be defined as relative center.
Preferably: said kind is a blood smear.Can also be that uterine neck section, marrow sheet, phlegm section and urine section etc. all can.
Preferably: said quality category comprises: automatic smear, semi-automatic smear and artificial smear any.
Preferably: said low power lens is 10 times of mirrors.
Preferably: said high power lens is oily mirror.According to different slide samples, high power lens can be selected 60 times of equimultiples.Low power lens is relative with high power lens, and the difference of object is selected low power lens and high power lens according to the observation.Some institutional framework is observed, and under 10 times of mirrors, observes earlier, can achieve the goal at 20 times of object lens then.
Preferably: said object lens turn to coarse adjustment under the high power lens, and wherein the focusing center of rough focusing is the cell that is positioned at the field-of-view image central area.
Preferably: said cell is the leucocyte in the blood smear.
This beneficial effect of the invention:
The present invention selects to focus by grade based on the field-of-view image pair cell based on the focusing grade that is provided with under high power lens after under the microscopical low power lens take pictures in the completion visual field voluntarily.This mode make system can be automatically according to the adjustment of focusing of the attribute characteristics of smear, within the specific limits, even the smear became uneven can not influence the readability of images acquired yet.Except the image of gathering was more clear, the zone of collection was also more extensive.
Description of drawings
Fig. 1 is the block scheme of one embodiment of the present of invention.
Fig. 2 is the image of A cell under 10 times of mirrors of blood smear.
Fig. 3 is the focusing center under the blood smear oil mirror among Fig. 2.
Fig. 4 is the image of the A cell under the blood picture oil mirror among Fig. 2.
Embodiment
The present invention proposes a kind of method of slide sample IMAQ, comprising:
Import the attribute of slide sample in advance to system: the kind and the quality category of slide sample are set in advance, and this classification supplies user to operate selection;
In advance to system's input focusing grade: the biomicroscope focusing grade that carries out image is gathered under high power lens is set in advance; The focusing grade comprises four grades: based on next width of cloth field-of-view image focusing of low power lens once; Focusing is a cell focusing to relative center, the visual field, again each cell in the visual field is carried out the collection of high power lens hypograph; With next width of cloth visual field separated into two parts of low power lens, these two parts all to be focused once, focusing is a cell focusing to relative center, the visual field, after every parts of images focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph; Next width of cloth visual field of low power lens is divided into three parts, each parts of images is all focused once, focusing is a cell focusing to relative center, the visual field, after each part focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph; Next width of cloth visual field of low power lens is divided into four parts, each parts of images is all focused once, focusing is a cell focusing to relative center, the visual field, after each part focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph;
The attribute of slide sample and the corresponding relation of focusing grade are set in advance;
The quantity of the cell image that needs collection is set in advance;
The user carries out the attribute selection of slide sample according to the attribute of slide sample;
Under the low power objective of biological microscope, slide sample is carried out the low power IMAQ, form the low-power field image;
Object lens turn to rough focusing under the high power lens;
Object lens are under high power lens after the coarse adjustment, and the attribute and the corresponding relation of the attribute that slide sample is set in advance with the focusing grade of the slide sample that system is selected according to the user are accordinged to the low-power field image automatically according to the focusing grade carries out image collecting work of correspondence;
The quantity of the image of the cell of gathering promptly stops IMAQ after reaching the quantity of cell image of the needs collection that is provided with in advance.
Technical scheme of the present invention is described below by way of example.
It is example that this example is selected blood smear for use.The difference in thickness of the blood smear of selecting for use is 0.01 ~ 0.2mm.The difference in thickness of smear is not limited to above-mentioned scope.
Get semi-automatic blood smear sample, the system's option to the biological microscope systemic presupposition has automatic blood smear, semi-automatic blood smear and three kinds of selections of manual blood smear earlier.It is semi-automatic blood smear that the user selects slide sample.
In advance to system's input focusing grade: set in advance the biomicroscope focusing grade that carries out image is gathered under high power lens; The focusing grade comprises four grades: focusing grade one, to view picture field-of-view image focusing under the low power lens once; Focusing is a cell focusing to relative center, the visual field, then each cell in the visual field is focused and collection image under high power lens; The focusing grade two, next width of cloth visual field of low power lens is divided into left and right sides two parts; These two parts are all focused once; Focusing is a cell focusing to relative center, the visual field, after every parts of images focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph; The focusing grade three, next width of cloth visual field of low power lens is divided into left, center, right three parts; Each parts of images is all focused once; Focusing is a cell focusing to relative center, the visual field, after each part focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph; Focusing grade four, next width of cloth visual field of low power lens is divided into upper left, lower-left, upper right and bottom right four parts; All focus once according to each parts of images; Focusing is a cell focusing to relative center, the visual field, after each part focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph.Each focusing all is cell focusing placed in the middle relatively in the visual field, one's respective area.
The attribute of slide sample and the corresponding relation of focusing grade are set in advance, the blood smear of semi-automatic smear are established correspondence be changed to focusing grade two.If as there being 5 leucocytes in the blood smear of sample, estimate to gather under 10 times of mirrors field-of-view image 39 width of cloth, the preset quantity of leucocyte that needs is 100 (or a plurality of).After possessing some and be provided with, the biology microscope mirror system can move.
Under 10 times of mirrors, take the field-of-view image of 39 width of cloth (also can be several) slide sample, and it is stored, piece image wherein is the location drawing of A cell under 10 times of mirrors referring to shown in Figure 2.System adjusts under object lens to the 100 times oily mirror then, and user's oil dripping can be reminded by system in the transfer process.At this moment microscope can wait step-length to move big displacement, is called for short rough focusing, and the purpose of rough focusing is to confirm the roughly focal plane of collection of cells, and the observation cell is in and knows state under the mirror.
In the time of rough focusing, system is an origin with a leucocyte of field-of-view image central area, carries out accurate focusing to this origin then; Referring to Fig. 3 is focusing center figure under the high power lens; All leucocytes in the system identification field-of-view image then are referring to A cell position figure shown in Figure 4, for example; There are five leucocytes in this visual field, and give the relative initial point of each leucocyte leukocytic coordinate figure.System carries out images acquired one by one to each leucocyte then, obtains each leukocytic picture rich in detail.After accomplishing the corresponding leucocyte IMAQ of a width of cloth field-of-view image, read second width of cloth field-of-view image then, system is 100 times of mirrors of coarse adjustment again, and accurate focusing is given each leucocyte coordinate then, then each leucocyte is carried out IMAQ.And the like, up to accomplishing 100 leukocytic IMAQs, can calculate thus, when accomplishing the 26th width of cloth field-of-view image, just accomplished 100 leukocytic IMAQ tasks, therefore, remaining 13 width of cloth are not used.Field-of-view image is in this process, as the effect of leukocytic coordinate navigation picture.System also can field-of-view image the upper left corner be origin, other leucocyte is given corresponding coordinate with reference to this initial point, but system need be with a leucocyte of the central area of the field-of-view image center as the visual field.
For the bigger smear of plate coating thickness difference, above-mentioned focusing grade two poor effect that may seem.Can select above-mentioned focusing grade three this moment for use, and field-of-view image is divided into three parts in left, center, right, respectively accurate focusing carried out in the zone of three parts, one by one the leucocyte in the scope carried out images acquired then.The blood smear of same type, original method can be suitable for 0.01 ~ 0.1mm thickness difference smear, and this example can obviously be suitable for the smear of 0.01 ~ 0.2mm thickness difference, under this thickness difference, can obtain distinct image in the same old way.
Being provided with of above-mentioned this focusing grade can make microscopic system be suitable for different section and quality types, makes the microscopical scope of application wider.
The kind of slide sample is except being the blood smear.Can also be uterine neck section, marrow sheet, phlegm section and urine section etc. all can, the grade that those skilled in the art can select high power lens and low power lens and select focusing based on method of the present invention for use based on existing techniques voluntarily.
The above results shows that the present invention compares prior art, and they are just like the property difference of following table:
| Comparison point | Original method | New method |
| Picture clarity | Low | High |
| Pickup area | Little | Greatly |
| The focusing mode | Once | Repeatedly |
| Gather plate coating thickness | Thin | Thickness all can |
Find out that by last table the present invention has produced that picture is clear, pickup area is wide and has gathered the bigger good result of thickness difference through the mode of focusing repeatedly.
Fig. 1 can be regarded as the simplification of the foregoing description and describe: the user selects the blood smear of semi-automatic processing; Under 10 times of mirrors, gather several field-of-view images and storage then; Carry out 100 times of oily mirror coarse adjustment again; Reading of data then, system select the user blood pictorial information of semi-automatic processing and focusing grade to mate, and produce the data of images acquired, and system reads this data, carries out subsequent action according to these data; System reads the field-of-view image of 10 times of mirrors shooting according to focusing grade and focusing of leucocyte areal distribution situation and images acquired; If the picture sum of gathering is less than the quantity of leucocyte of preset requirement then continue to read the field-of-view image of taking 10 times of mirrors under, the leucocyte of the field-of-view image of taking up to 10 times of all mirrors reads accomplishes just end; If the picture sum of gathering more than or equal to the total white blood cells of preset requirement, then finishes to gather.
Claims (7)
1. the method for a slide sample IMAQ comprises:
Import the attribute of slide sample in advance to the system of biological microscope: the kind and the quality category of slide sample are set in advance, and this classification supplies user to operate selection;
In advance to system's input focusing grade: the biomicroscope focusing grade that carries out image is gathered under high power lens is set in advance; The focusing grade comprises four grades: based on next width of cloth field-of-view image focusing of low power lens once; Focusing is a cell focusing to relative center, the visual field, again each cell in the visual field is carried out the collection of high power lens hypograph; With next width of cloth visual field separated into two parts of low power lens, these two parts all to be focused once, each focusing all is cell focusing placed in the middle relatively in the visual field, one's respective area, after every parts of images focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph; Next width of cloth visual field of low power lens is divided into three parts, each parts of images is all focused once, each focusing all is cell focusing placed in the middle relatively in the visual field, one's respective area, after each part focusing is accomplished, promptly the cell in this part is carried out the high power lens IMAQ; Next width of cloth visual field of low power lens is divided into four parts, each parts of images is all focused once, each focusing all is cell focusing placed in the middle relatively in the visual field, one's respective area, after each part focusing is accomplished, promptly the cell in this part is carried out the collection of high power lens hypograph;
The attribute of slide sample and the corresponding relation of focusing grade are set in advance;
The quantity of the cell image that needs collection is set in advance;
The user carries out the attribute selection of slide sample according to the attribute of slide sample;
Under the low power objective of biological microscope, slide sample is carried out the low power IMAQ, form the low-power field image;
Object lens turn to rough focusing under the high power lens;
Object lens are under high power lens after the coarse adjustment, and the attribute and the corresponding relation of the attribute that slide sample is set in advance with the focusing grade of the slide sample that system is selected according to the user are accordinged to the low-power field image automatically according to the focusing grade carries out image collecting work of correspondence;
The quantity of the image of the cell of gathering promptly stops IMAQ after reaching the quantity of cell image of the needs collection that is provided with in advance.
2. the method for slide sample IMAQ according to claim 1 is characterized in that: said kind is a blood smear.
3. the method for slide sample IMAQ according to claim 1 is characterized in that: said quality category comprises: automatic smear, semi-automatic smear and artificial smear any.
4. the method for slide sample IMAQ according to claim 1 is characterized in that: said low power lens is 10 times of mirrors.
5. the method for slide sample IMAQ according to claim 1 is characterized in that: said high power lens is oily mirror.
6. the method for slide sample IMAQ according to claim 1 is characterized in that: said object lens turn to coarse adjustment under the high power lens, and wherein the focusing center of rough focusing is the cell that is positioned at the field-of-view image central area.
7. the method for slide sample IMAQ according to claim 2 is characterized in that: said cell is the leucocyte in the blood smear.
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| CN103175834B (en) * | 2013-01-28 | 2016-05-18 | 宁波江丰生物信息技术有限公司 | A kind of digital pathological section quality determining method and system |
| CN105223110B (en) * | 2014-06-27 | 2018-10-30 | 苏州惠生电子科技有限公司 | A kind of microscope locating and tracking imaging method, device and urinal system |
| CN105067520B (en) * | 2015-07-28 | 2018-10-23 | 爱威科技股份有限公司 | A kind of microscopy recognition methods and device |
| CN115032411A (en) * | 2016-07-29 | 2022-09-09 | 希森美康株式会社 | Specimen transport device, specimen image capturing system, and specimen analysis system |
| CN110231259B (en) * | 2019-05-28 | 2022-02-18 | 怀光智能科技（武汉）有限公司 | Digital diagnosis system for cervical cell slide |
| CN114152610B (en) * | 2021-11-02 | 2023-06-27 | 桂林优利特医疗电子有限公司 | Slide cell scanning method based on visual target mark |
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