CN209460143U - The Rapid identification instrument of pathogenic microorganism - Google Patents
The Rapid identification instrument of pathogenic microorganism Download PDFInfo
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- CN209460143U CN209460143U CN201821942926.2U CN201821942926U CN209460143U CN 209460143 U CN209460143 U CN 209460143U CN 201821942926 U CN201821942926 U CN 201821942926U CN 209460143 U CN209460143 U CN 209460143U
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Abstract
The utility model relates to the Rapid identification instruments of pathogenic microorganism, belong to microbial identification technical field.The Rapid identification instrument of pathogenic microorganism includes white light imaging unit and Raman cell, white light imaging unit is for shooting sample white light picture, realize cell automatic identification and positioning, Raman cell obtains the fingerprint characteristic of pathogenic microorganism cell for measuring pathogenic microorganism cell Raman spectrum, when shooting white light picture identification cell, white light imaging unit moves into optical path, and Raman cell removes optical path, when measuring Raman signal, white light imaging unit removes optical path, and Raman cell moves into optical path.Compared with prior art, the utility model substantially reduces the time of microbial identification due to not needing to increase bacterium and be separately cultured.In the instrument using the utility model is tested, most only need a few minutes that the getable result of original 1-3 talent can be obtained fastly.
Description
Technical field
The utility model relates to microbial identification technical fields, more particularly, to the Rapid identification instrument of pathogenic microorganism.
Background technique
The identification of pathogenic microorganism is mainly divided to culture and does not cultivate two general orientation in existing urine.The work identified after incubation
Make in process, the pathogenic microorganism in urine obtains pure bacterial strain through 16-40 hours culture of isolated, can directly pass through mass spectrum later
Mode identifies microorganism kind, or by being sequenced after DNA cloning；Or by biochemical reaction, one is cultivated under different substrates
(16-40 hours usual) judges most possible kind classification results according to the metabolic characteristics to different substrates after the section time.
In the workflow without culture, there is the immune reagent kit for certain class pathogen that can more be rapidly performed by identification.
In the above-mentioned methods, the method identified after cultivation, the time is longer, could usually go out at 1-2 days as a result, and passing through
The method cost of sequencing is very high, and the requirement to operator is also higher.The method limitation of immune reagent kit is very big, and every kind immune
Kit can only be directed to extremely limited pathogenic microorganism type.
Chinese patent CN1606691A describes the cell quickly and delicately identified in medicine, industry and environmental sample
With the effective ways of viral target.Then first labels targets detect them using large area imaging.The difficult point of this method is to need elder generation
Labels targets, then detected.
Utility model content
The purpose of this utility model is exactly to provide pathogenic microorganism to overcome the problems of the above-mentioned prior art
Rapid identification instrument.
The purpose of this utility model can be achieved through the following technical solutions:
The Rapid identification instrument of the utility model offer pathogenic microorganism.
It is provided by the utility model that the Rapid identification instrument of pathogenic microorganism is provided, comprising:
White light imaging unit: for shooting sample white light picture, cell automatic identification and positioning are realized；
Raman cell: for measuring pathogenic microorganism cell Raman spectrum, the fingerprint characteristic of pathogenic microorganism cell is obtained；
When shooting white light picture identification cell, white light imaging unit moves into optical path, and Raman cell removes optical path, when measurement is drawn
When graceful signal, white light imaging unit removes optical path, and Raman cell moves into optical path.
In an embodiment of the utility model, the Rapid identification instrument of the pathogenic microorganism further includes for putting
Set the glass slide of sample, and the auto-translating platform for moving glass slide.
The glass slide, also referred to as chip are provided with a label, for marking for placing sample on glass slide
Remember coordinate origin.
The glass slide is advisable with can more preferably reduce laser reflection while enhance the material of Raman signal collection or structure,
Such as quartz, calcirm-fluoride or coat of metal material.
In an embodiment of the utility model, the Rapid identification instrument of the pathogenic microorganism further include white light at
The object lens shared as unit and Raman cell.
In an embodiment of the utility model, the white light imaging unit includes white light, semi-transparent semi-reflecting eyeglass
Group and camera,
The white light is used to issue light,
The semi-transparent semi-reflecting eyeglass group is passed through for being focused on sample after reflecting light through object lens, and by sample scattering light
Object lens are collected back reflection and are gone out,
The camera is used to receive the light of semi-transparent semi-reflecting eyeglass group reflection, records image.
In an embodiment of the utility model, the semi-transparent semi-reflecting eyeglass group includes two semi-transparent semi-reflecting lens, white
The light that light lamp issues focuses on sample after first semi-transparent semi-reflecting lens reflects, then through object lens, and sample scattering light is received through object lens
Collection, transmitted through after first semi-transparent semi-reflecting lens after second semi-transparent semi-reflecting lens reflect, by cameras record image.
In an embodiment of the utility model, the Raman cell includes:
Grating: Raman signal for generating sample under laser action by Wavelength distribution by different angle diffraction,
Detector: for collecting and recording the Raman spectrum after grating beam splitting.
In an embodiment of the utility model, the Raman cell further includes laser emission element, the laser
Transmitting unit includes laser, laser narrow-band optical filter, and the laser is used for generating laser, the laser narrow-band optical filter
In filtering out the miscellaneous line in laser.
In an embodiment of the utility model, the laser be 488nm laser, 514nm laser,
One of 532nm laser, 633nm laser or 785nm laser.
In an embodiment of the utility model, the usually optional 532/2nm of laser narrow-band optical filter is to filter out laser
In miscellaneous line, transmitance is greater than 90% at 532nm, transmittance curve full width at half maximum 2nm.
In an embodiment of the utility model, the laser emission element further includes laser power attenuator, is used
In carrying out different degrees of decaying to laser intensity, to adjust laser intensity.
In an embodiment of the utility model, the optional 6 groups of attenuators of laser power attenuator are to laser intensity
Different degrees of decaying is carried out, to better meet protection sample and enhance the function of Raman signal.
In an embodiment of the utility model, the Raman cell further includes Raman optical filter, the Raman filter
Mating plate, which is used to reflect after the laser reflection that microscope group launches, focuses to sample by object lens, when measuring Raman signal, institute
Object lens are stated for collecting the Raman scattering signal and Rayleigh scattering signal of sample scattering, and make Raman scattering signal and Rayleigh scattering
Signal returns to Raman optical filter, and the Rayleigh scattering signal that the Raman optical filter is also used to that sample is stopped to scatter is dissipated through sample
The Raman scattering signal penetrated.
In an embodiment of the utility model, the Raman cell further includes being copolymerized burnt pin hole, slit,
The burnt pin hole of the copolymerization and slit are arranged at Raman optical filter rear, for projecting grating after passing through laser
On, the grating is reflective gratings.
The burnt pin hole of the copolymerization under confocal mode for stopping spurious signal, and promotion sample signal is strong under non-confocal mode
Degree.
The burnt pin hole of the copolymerization by adjusting aperture size, reach stop under confocal mode spurious signal, improve sample at
The three-dimensional space resolution ratio of picture promotes the purpose of sample signal intensity under non-confocal mode；Grating is by Raman signal by wavelength point
Cloth presses different angle diffraction.
In an embodiment of the utility model, the detector selects EMCCD detector, to record grating point
Raman spectrum after light.
In an embodiment of the utility model, the Raman cell further includes reflection microscope group, the reflection microscope group
Including the first reflecting mirror and the second reflecting mirror.
When measuring Raman signal, exciting light is issued through laser, filters out miscellaneous line by laser narrow-band optical filter, then through swashing
After optical power attenuation piece adjusting strength, the incident angle after being optimized laser by reflecting mirror is projected to Raman optical filter；Laser
It is focused on sample after the reflection of Raman optical filter by object lens；Auto-translating platform can be according to software under white light imaging for cell
The recognition result of coordinate automatically moves sample and cell is moved to the focal point of object lens and acquires Raman spectrum, to reach scanning
Purpose is imaged；By Raman scattering signal (Raman signal is orange) and Rayleigh scattering signal (laser signal green) quilt of sample scattering
Return Raman optical filter after object lens are collected, is blocked in this Rayleigh scattering signal and Raman scattering signal penetrates Raman optical filter；
Raman scattering signal projects on reflective gratings after being collimated using the burnt aperture of copolymerization and slit by concave mirror, through optical grating diffraction
Assemble again through another concave mirror to detector by the directional light that wavelength is arranged afterwards, final output Raman signal.
In an embodiment of the utility model, the Rapid identification instrument of the pathogenic microorganism further includes at data
Equipment is managed,
The data processing equipment realizes cell certainly for analyzing the sample white light picture that white light imaging unit is shot
It is dynamic to identify and position；And it analyzed, compared and is judged for Raman spectrum obtained to Raman cell, identify the micro- life of cause of disease
Object.
In an embodiment of the utility model, the data processing equipment is computer, the computer and photograph
Camera, detector, the connection of auto-translating platform.
In an embodiment of the utility model, the Rapid identification instrument of the pathogenic microorganism is mainly used for urine
The Rapid identification of middle pathogenic microorganism, i.e. the Rapid identification instrument as pathogenic microorganism in kind of urine use.
The technical program is the first equipment with single cell Raman spectrum to being identified including microorganism in urine.With it is existing
There is technology to compare, due to not needing to increase bacterium and be separately cultured, substantially reduces the time of microbial identification.Practical new using this
During the instrument of type is tested, most only need a few minutes that the getable result of original 1-3 talent can be obtained fastly.
Detailed description of the invention
Fig. 1 is to use structural schematic diagram when white light imaging cell operation；
Fig. 2 is structural schematic diagram when being worked using Raman cell；
Fig. 3 is to carry out principal component analysis result to collected spectrum using LDA function in embodiment 3.
Shown in figure label: 1, laser, 2, laser narrow-band optical filter, 3, laser power attenuator, the 4, first reflecting mirror,
5, the second reflecting mirror, 6, Raman optical filter, 7, object lens, 8, glass slide, 9, auto-translating platform, 10, the burnt pin hole of copolymerization, 11, slit,
12, grating, 13, detector, 14, white light, 15 first semi-transparent semi-reflecting eyeglasses, 16, second semi-transparent semi-reflecting eyeglasses, 17, photograph
Camera.
Specific embodiment
The utility model is described in detail in the following with reference to the drawings and specific embodiments.
Embodiment 1
The Rapid identification instrument of pathogenic microorganism is provided.
It is provided in this embodiment that the Rapid identification instrument of pathogenic microorganism is provided with reference to Fig. 1, Fig. 2, comprising:
White light imaging unit: for shooting sample white light picture, cell automatic identification and positioning are realized；
Raman cell: for measuring pathogenic microorganism cell Raman spectrum, the fingerprint characteristic of pathogenic microorganism cell is obtained；
For placing the glass slide 8 of sample, and the auto-translating platform 9 for moving glass slide 8；
The object lens 7 that white light imaging unit and Raman cell share；
Data processing equipment: the data processing equipment is for dividing the sample white light picture that white light imaging unit is shot
Cell automatic identification and positioning are realized in analysis；And it analyzed, compared and is judged for Raman spectrum obtained to Raman cell,
Identify pathogenic microorganism.
When shooting white light picture identification cell, white light imaging unit moves into optical path, and Raman cell removes optical path, with reference to Fig. 1,
When measuring Raman signal, white light imaging unit removes optical path, and Raman cell moves into optical path, with reference to Fig. 2.
In the present embodiment, the white light imaging unit includes white light 14, semi-transparent semi-reflecting eyeglass group and camera 17, described
White light 14 focuses on sample for issuing light after the semi-transparent semi-reflecting eyeglass group is used to reflect light through object lens 7, and will
Sample scatters light and goes out through the collection back reflection of object lens 7, and the camera 17 is used to receive the light of semi-transparent semi-reflecting eyeglass group reflection, record figure
Picture.
Wherein, the semi-transparent semi-reflecting eyeglass group includes two semi-transparent semi-reflecting lens, and the light that white light 14 issues is through first half
It after saturating semi-reflective mirror 15 reflects, then focuses on sample through object lens 7, sample scattering light is collected through object lens 7, transmitted through first half
After saturating semi-reflective mirror 15 after second semi-transparent semi-reflecting lens 16 reflects, image is recorded by camera 17.
In the present embodiment, the Raman cell includes:
Laser emission element, the laser emission element include laser 1, laser narrow-band optical filter 2, laser power decaying
Piece 3, the laser 1 are used to filter out the miscellaneous line in laser, laser power for generating laser, the laser narrow-band optical filter 2
Attenuator 3, for carrying out different degrees of decaying to laser intensity, to adjust laser intensity；
Reflect microscope group: the reflection microscope group includes the first reflecting mirror 4 and the second reflecting mirror 5；
Raman optical filter 6: the Raman optical filter 6 will be for that will reflect after the laser reflection that microscope group launches by object lens 7
It focuses on sample, when measuring Raman signal, the object lens 7 are used to collect the Raman scattering signal of sample scattering and Rayleigh dissipates
Signal is penetrated, and Raman scattering signal and Rayleigh scattering signal is made to return to Raman optical filter 6, the Raman optical filter 6 is also used to hinder
The Rayleigh scattering signal for keeping off sample scattering, through the Raman scattering signal of sample scattering:
Be copolymerized burnt pin hole 10, slit 11: the burnt pin hole 10 of the copolymerization is arranged with slit 11 at 6 rear of Raman optical filter, uses
It is projected after passing through laser on grating 12；
Grating 12: for making the Raman signal of sample generation under laser action by Wavelength distribution by different angle diffraction, institute
Stating grating 12 is reflective gratings；
Detector 13: for collecting and recording the Raman spectrum after grating beam splitting.
Wherein, the laser 1 be 488nm laser, 514nm laser, 532nm laser, 633nm laser or
One of 785nm laser.The usually optional 532/2nm of laser narrow-band optical filter 2 is to filter out the miscellaneous line in laser, at 532nm
Transmitance is greater than 90%, transmittance curve full width at half maximum 2nm.3 optional 6 groups of attenuators of laser power attenuator are to laser intensity
Different degrees of decaying is carried out, to better meet protection sample and enhance the function of Raman signal.The burnt pin hole 10 of the copolymerization
For stopping spurious signal under confocal mode, sample signal intensity is promoted under non-confocal mode.The burnt pin hole 10 of the copolymerization is logical
Aperture size is overregulated, the three-dimensional space resolution ratio for reaching and stopping spurious signal under confocal mode, improve sample imaging, non-confocal
The purpose of sample signal intensity is promoted under mode；Raman signal is pressed different angle diffraction by Wavelength distribution by grating 12.The spy
It surveys device 13 and selects EMCCD detector, to record the Raman spectrum after grating beam splitting.
When measuring Raman signal, exciting light is issued through laser, filters out miscellaneous line by laser narrow-band optical filter, then through swashing
After optical power attenuation piece adjusting strength, the incident angle after being optimized laser by reflecting mirror is projected to Raman optical filter；Laser
It is focused on sample after the reflection of Raman optical filter by object lens；Auto-translating platform can be according to software under white light imaging for cell
The recognition result of coordinate automatically moves sample and cell is moved to the focal point of object lens and acquires Raman spectrum, to reach scanning
Purpose is imaged；By Raman scattering signal (Raman signal is orange) and Rayleigh scattering signal (laser signal green) quilt of sample scattering
Return Raman optical filter after object lens are collected, is blocked in this Rayleigh scattering signal and Raman scattering signal penetrates Raman optical filter；
Raman scattering signal projects on reflective gratings after being collimated using the burnt aperture of copolymerization and slit by concave mirror, through optical grating diffraction
Assemble again through another concave mirror to detector by the directional light that wavelength is arranged afterwards, final output Raman signal.
In the present embodiment, the glass slide 8, also referred to as chip are provided with one on glass slide 8 for placing sample
A label, for marking coordinate origin.The glass slide 8 is collected with can more preferably reduce laser reflection while enhance Raman signal
Material or structure be advisable, such as quartz, calcirm-fluoride or coat of metal material.
In the present embodiment, the data processing equipment is computer, the computer and camera 17, detector 13, from
Dynamic translation stage 9 connects.
In the present embodiment, the Rapid identification instrument of the pathogenic microorganism is mainly used for the quick of pathogenic microorganism in urine
Identification, i.e. the Rapid identification instrument as pathogenic microorganism in kind of urine use.
Embodiment 2
The rapid identification method of pathogenic microorganism is carried out using the instrument that embodiment 1 provides, comprising the following steps:
A, white light imaging unit photographs sample white light picture realizes cell automatic identification and positioning；
B, after cellular localization, cell Raman spectrum is measured using Raman cell, obtains the Raman light of pathogenic microorganism cell
Spectrum, i.e., spectrum to be identified；
C, the Raman spectrum of spectrum to be identified and known multiple-microorganism is compared, determines pathogenic microorganism to be detected
Kind.
In the present embodiment, Rapid identification of the method for pathogenic microorganism in urine.
In the present embodiment, before carrying out Rapid identification to pathogenic microorganism in urine, centrifuge washing is passed through to urine sample
Mode carries out pre-treatment.
In the present embodiment, the pre-treating method of urine sample are as follows:
50ul-1ml urine is taken, in the culture medium that 2 times of isometric concentration is wherein added.Culture medium is usually MH culture
Base.Later under the conditions of 35-37 DEG C, 0-16h is cultivated.Sample is washed by way of centrifugation later, specifically:
Be centrifuged 2-10min under 3000-10000rpm revolving speed, after discarding supernatant, be added certain volume deionized water or distilled water or
Person's ultrapure water blows and beats uniformly resuspension by oscillation or with liquid-transfering gun, is centrifuged again by above-mentioned condition, discards supernatant lay equal stress on again
It is outstanding.It can repeat 0-2 times later.The final sample being resuspended in water, takes on certain volume (0.2ul-10ul) to glass slide, from
After so drying or dry, the Rapid identification instrument setup test of pathogenic microorganism is moved to.
In the present embodiment, step A's method particularly includes:
There is a label for marking coordinate origin on A1, glass slide, the Rapid identification instrument of pathogenic microorganism first can
The position of mark point in correct for slide, auto-translating platform 9 drives glass slide mobile later, the Rapid identification instrument of pathogenic microorganism
The glass slide that record is loaded with sample by device mobile direction and distance；
A2, using camera shooting picture before, camera imaging size need to be by the standard size sample of micron level
Correction, to record the size of the corresponding phase of camera chip single pixel, camera chip refers to the CCD or CMOS of camera, thus
The size of captured article is read in subsequent shooting sample photo；
In A3, unicellular automatic identification and position fixing process, camera 17 shoots sample white light picture, and cell and impurity particle are equal
It is recorded in image file, by analyzing the contrast of image, brightness can distinguish background and sample, pass through analysis sample
Size, surface smooth degree, shape etc. can be separated cell by pre-set screening conditions from impurity,
And the direction and range information mobile according to coordinate origin and glass slide, obtain the two-dimensional coordinate of cell present position.
Wherein, the contrast of image is analyzed, the embedded software reality of background and sample by data processing equipment is distinguished in brightness
It is existing, it analyzes size, surface smooth degree, shape of sample etc. and is realized by the embedded software of data processing equipment, and
Screening conditions are preset on data processing equipment, these technologies can be realized with existing technological means, for example,
The image processing algorithms such as Sobel/Prewitt.
In an embodiment of the utility model, step B's method particularly includes:
Label laser measurement point (has a fixed position, laser is focused to the fixed bit on the chip of test sample
Set, the coordinate of the point be set as (0,0)), and selected target measurement cell is (under white light imaging, due to known coordinate origin
(0,0) it is also known that lower per unit is imaged apart from corresponding space actual range (on CCD), therefore can provide each under imaging
Coordinate of the cell relative to (0,0), when needing to test Raman spectrum, according to this coordinate, mobile laser point/or focusing
On central point to this target cell), according to white light imaging unit photographs sample white light picture, realize cell automatic identification and positioning
Afterwards, according to the cell coordinate position of record, each cell, which corresponds to laser measurement point, will obtain a motion-vector parameter, at data
Equipment triggering auto-translating platform is managed according to each motion-vector parameter, target cell is individually successively moved to laser measurement point,
And issue one by auto-translating platform immediately and adopt spectrum trigger signal to detector, instruct what detector completed the cell to adopt spectrum, such as
This repeated several times, completes the micro-imaging of microbial single-cell, and each sample should acquire 10-200 single celled Raman figures
Spectrum, is denoted as spectrum to be identified.
In the present embodiment, the Raman spectrum of known multiple-microorganism is to be directed to known multiple-microorganism in advance in step C,
Using resulting Raman spectrum after the Rapid identification instrument detection of pathogenic microorganism, and pass through supervised
Classification is classified mark to the strain of microorganism, kind, category etc., and with support vector machine,
k-nearest neighbour、Linear Discriminant Analysis、Convolutional Neural
Networks scheduling algorithm is extracted the feature of classification.
Embodiment 3
Fresh urine 20ml is acquired, is 0.22 μm of membrane filtration with aperture.The bacterium below LB liquid medium kind culture
Strain, Pseudomonas aeruginosa (ATCC 27853), Enterococcus faecalis (ATCC29212),
Klebsiella pneumoniae (ATCC 700603), Staphylococcus epidermidis (ATCC12228),
Escherichia coli (ATCC 25922), Acinetobacter baumannii, (ATCC 19606) are stayed overnight.
The bacterium solution 1ml, 5000rpm that are incubated overnight is taken to be centrifuged 2min, the sterile water gun nozzle piping and druming of 1ml, which is added, is resuspended precipitating,
It is seeded to filtered urine with 1:1000 ratio, cultivates 0h-2h in 37 DEG C of shaking tables.
1ml urine is taken, 5000rpm is centrifuged 2min, and the sterile water gun nozzle piping and druming of 1ml, which is added, is resuspended precipitating, repeats the process
1-3 all over after, add 50 μ l sterile waters be resuspended, using embodiment 1 equipment and embodiment 2 method to single celled Raman spectrum into
Row acquisition, amounts to 903 by every kind bacterium spectra collection quantity 51-302.
In R software (version 3 .4.3), utilize ' mass ' software package in LDA function to collected spectrum into
The result of row principal component analysis, each bacterium classification is as shown in Figure 3, it can be seen that different strains rough can be distinguished.It utilizes
Under ' mass ' data set ' predict ' order, the Raman spectrum of variety classes bacterium is identified, and with truth pair
Than the accuracy rate of available identification, as shown in table x.
It can understand the above description of the embodiments is intended to facilitate those skilled in the art and use practical
It is novel.Person skilled in the art obviously easily can make various modifications to these embodiments, and illustrating herein
General Principle be applied in other embodiments without having to go through creative labor.Therefore, the utility model is not limited to above-mentioned
Embodiment, those skilled in the art's announcement according to the present utility model, do not depart from improvement that the utility model scope is made and
Modification should be all within the protection scope of the utility model.
Claims (8)
1. the Rapid identification instrument of pathogenic microorganism characterized by comprising
White light imaging unit: for shooting sample white light picture, cell recognition and positioning are realized；
Raman cell: for measuring pathogenic microorganism cell Raman spectrum, the fingerprint characteristic of pathogenic microorganism cell is obtained；It is described
Raman cell includes grating (12) and detector (13), grating (12) be used to making sample generates under laser action Raman signal by
Wavelength distribution presses different angle diffraction, and detector (13) is for collecting and recording the Raman spectrum after grating beam splitting；
For placing the glass slide (8) of sample, and,
For moving the auto-translating platform (9) of glass slide (8), and,
The object lens (7) of white light imaging unit and Raman cell；
When shooting white light picture identification cell, white light imaging unit moves into optical path, and Raman cell removes optical path, when measurement Raman letter
Number when, white light imaging unit remove optical path, Raman cell move into optical path.
2. the Rapid identification instrument of pathogenic microorganism according to claim 1, which is characterized in that the white light imaging unit packet
White light (14), semi-transparent semi-reflecting eyeglass group and camera (17) are included,
The white light (14) is used to issue light,
The semi-transparent semi-reflecting eyeglass group scatters light through object for being focused on sample after reflecting light through object lens (7), and by sample
Mirror (7) is collected back reflection and is gone out,
The camera (17) is used to receive the light of semi-transparent semi-reflecting eyeglass group reflection, records image.
3. the Rapid identification instrument of pathogenic microorganism according to claim 1, which is characterized in that the Raman cell further includes
Laser emission element, the laser emission element include laser (1), laser narrow-band optical filter (2), and the laser (1) is used
In generating laser, the laser narrow-band optical filter (2) is used to filter out the miscellaneous line in laser.
4. the Rapid identification instrument of pathogenic microorganism according to claim 3, which is characterized in that the laser (1) is
One of 488nm laser, 514nm laser, 532nm laser, 633nm laser or 785nm laser.
5. the Rapid identification instrument of according to claim 1 or 2 or 3 or 4 pathogenic microorganisms, which is characterized in that the Raman
Unit further includes Raman optical filter (6), the Raman optical filter (6) for will reflect after the laser reflection that microscope group launches by
Object lens (7) focus on sample, and when measuring Raman signal, the object lens (7) are used to collect the Raman scattering letter of sample scattering
Number and Rayleigh scattering signal, and Raman scattering signal and Rayleigh scattering signal is made to return to Raman optical filter (6),
The Rayleigh scattering signal that the Raman optical filter (6) is also used to that sample is stopped to scatter, through the Raman scattering of sample scattering
Signal.
6. the Rapid identification instrument of pathogenic microorganism according to claim 5, which is characterized in that the Raman cell further includes
It is copolymerized burnt pin hole (10), slit (11),
The burnt pin hole (10) of the copolymerization and slit (11) are arranged at Raman optical filter (6) rear, for projecting after passing through laser
To on grating (12),
The burnt pin hole (10) of the copolymerization under confocal mode for stopping spurious signal, and promotion sample signal is strong under non-confocal mode
Degree.
7. the Rapid identification instrument of pathogenic microorganism according to claim 1, which is characterized in that further include that data processing is set
It is standby,
The data processing equipment realizes that cell is known automatically for analyzing the sample white light picture that white light imaging unit is shot
Not and position；And it analyzed, compared and is judged for Raman spectrum obtained to Raman cell, identify pathogenic microorganism.
8. the Rapid identification instrument of pathogenic microorganism according to claim 7, which is characterized in that the data processing equipment is
Computer, the computer are connect with camera (17), detector (13), auto-translating platform (9).
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| CN201821942926.2U CN209460143U (en) | 2018-11-23 | 2018-11-23 | The Rapid identification instrument of pathogenic microorganism |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110702664A (en) * | 2019-10-31 | 2020-01-17 | 上海氘峰医疗器械有限公司 | Method for rapidly identifying gram-negative and gram-positive bacteria |
| CN113983632A (en) * | 2021-11-16 | 2022-01-28 | 武汉工程大学 | Combined air conditioning unit purifying and sterilizing device and method |
| US11358984B2 (en) | 2018-08-27 | 2022-06-14 | Regeneran Pharmaceuticals, Inc. | Use of Raman spectroscopy in downstream purification |
-
2018
- 2018-11-23 CN CN201821942926.2U patent/CN209460143U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11358984B2 (en) | 2018-08-27 | 2022-06-14 | Regeneran Pharmaceuticals, Inc. | Use of Raman spectroscopy in downstream purification |
| CN110702664A (en) * | 2019-10-31 | 2020-01-17 | 上海氘峰医疗器械有限公司 | Method for rapidly identifying gram-negative and gram-positive bacteria |
| CN113983632A (en) * | 2021-11-16 | 2022-01-28 | 武汉工程大学 | Combined air conditioning unit purifying and sterilizing device and method |
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