CN211510154U

CN211510154U - Plant tissue culture container

Info

Publication number: CN211510154U
Application number: CN201921834995.6U
Authority: CN
Inventors: 李丹青; 张佳平; 夏宜平; 王小斌; 张栋; 邵灵梅
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2019-10-29
Filing date: 2019-10-29
Publication date: 2020-09-18
Anticipated expiration: 2029-10-29

Abstract

The utility model belongs to the technical field of the laboratory glassware, especially, relate to a plant tissue culture container. The container comprises a container body and a cover body, wherein the container body is a cylindrical bottle with an open top surface, a plurality of concentric annular grooves are arranged on the inner bottom surface of the container body, and partition rings are arranged in the annular grooves; the cover body is arranged on the container body. The utility model improves the test efficiency and scientificity; the body, the cover body and the separating ring are made of polypropylene materials, so that the cost is low, and the high-pressure sterilization device can also be used for high-pressure sterilization. In conclusion, the plant tissue culture vessel has extremely high practicability.

Description

Plant tissue culture container

Technical Field

The utility model belongs to the technical field of the laboratory glassware, especially, relate to a plant tissue culture container.

Background

The plant tissue culture can be used for quickly constructing a clone to obtain neat and consistent plant products, carrying out stem tip detoxification to obtain virus-free plants, and can also be used for constructing a genetic transformation system to obtain transgenic plants. However, a large number of repeated experiments are required for obtaining the optimal explant surface disinfection mode, culture medium formula and the like, and the operation flow comprises a plurality of steps: preparing culture medium, subpackaging into culture containers, sterilizing at high temperature and high pressure, inoculating, etc. The proper culture container can greatly improve the working efficiency and the quality.

At present, a culture dish or a cylindrical bottle is mostly adopted for plant tissue culture as a culture container. When using the culture dish as culture vessel, need carry out the partial shipment to the culture dish on superclean bench after the culture medium carries out high temperature autoclaving earlier, the partial shipment will go on when the culture medium does not solidify, because the temperature is higher, the operation is more difficult, scalds the staff easily or spills the culture medium to superclean bench or culture dish outside and causes the pollution, still need wrap up the culture dish for use after the partial shipment is accomplished, the step is loaded down with trivial details, and efficiency is lower. The cylindrical bottle is adopted to culture the plant tissue, so that the defects can be avoided, but the cylindrical bottle usually occupies a larger space in the processes of autoclaving and later culture, and particularly does not need a large space for growth in the callus induction or plant tissue bud induction period, so that the resource occupation and waste are caused. In addition, the cylindricality bottle is compared the culture dish and is cultivateed, and the air permeability is often relatively poor, and the comdenstion water appears easily in the bottle wall, influences the observation and the shooing in later stage. For strict scientific experiments, the culture medium volume in each treatment is required to be consistent, and if a common culture dish or a cylindrical bottle is adopted as a culture container, the culture dish or the cylindrical bottle is bound to be matched with measuring instruments such as a measuring cylinder for use, so that the workload is increased, and more possibility of causing culture medium pollution is generated. Therefore, a plant tissue culture vessel has been proposed to address the above problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a simple structure, low cost, convenient to use's plant tissue culture container overcomes prior art's is not enough in order to improve plant tissue culture work efficiency and quality.

For solving the defect that exists among the prior art, the utility model discloses a following technical scheme realizes above-mentioned purpose:

providing a plant tissue culture container, which comprises a container body and a cover body, wherein the container body is a cylindrical bottle with an open top surface, a plurality of concentric annular grooves are arranged on the inner bottom surface of the container body, and separating rings are arranged in the annular grooves; the cover body is arranged on the container body.

As an improvement, the top of the outer wall of the container body and the inner wall of the cover body are provided with matched threads for connecting the container body and the cover body through the threads.

As an improvement, a circle of vent ring with the width of 1cm is arranged on the container body below the thread, and an activated carbon fiber membrane serving as a breathable filter membrane is arranged on the inner side of the vent ring.

As a modification, the height of the annular groove is 2 mm.

As an improvement, the outer wall of the container body is also provided with volume scale marks such as 5mL, 10mL, 15mL and the like and a frosted mark area, and the frosted mark area is used for marking information such as sample names, serial numbers, test dates and batches.

As an improvement, the volume of the container body is 0.1-1.0L.

As an improvement, the container body, the cover body and the separating ring are made of polypropylene.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model can ensure the good sealing performance of the container through the thread design of the outer side of the bottle mouth of the body and the inner side of the cover body, and simultaneously avoid sealing by using a sealing film or a preservative film and the like, thereby reducing the workload and reducing the later pollution rate; the lower end of the bottle mouth is provided with the circle of ventilation ring and the ventilation filter membrane is matched at the inner side, so that the filter membrane can be protected, compared with a container only provided with one ventilation hole on the bottle cap, the ventilation performance is better, the generation of condensed water can be avoided, and the shooting from the cover body and the recording of the growth state of plant materials are not influenced; the side wall of the body is provided with marks and scale marks, so that the test record can be facilitated, and the consistency of the volume of the culture medium in each bottle of container can be ensured; the bottle bottom groove is matched with the separating ring to separate the container into different areas, and the container is used for a contrast test with strictly consistent test conditions, so that the test efficiency and the test scientificity are improved; the body, the cover body and the separating ring are made of polypropylene materials, so that the cost is low, and the high-pressure sterilization device can also be used for high-pressure sterilization. In conclusion, the plant tissue culture vessel has extremely high practicability.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Description of reference numerals: 1-container body, 2-cover body, 3-cover body inner side thread, 4-bottle mouth outer side thread, 5-body vent ring, 6-breathable filter membrane, 7-scale mark, 8-frosted mark area, 9-annular groove and 10-separating ring.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the accompanying drawings and the embodiments.

As shown in fig. 1, the present embodiment includes a container body 1 and a lid body 2, the container body 1 is a cylindrical bottle with an open top surface, the lid body 2 is engaged with the outer thread 4 of the mouth of the container body 1 by an inner thread 3, and the bottom of the container body 1 is provided with a plurality of concentric annular grooves 9 with a height of 2mm for placing a separation ring 10 to separate the space in the container. The lower end of the thread 4 of the container body 1 is provided with a vent ring 5, a ventilation filter membrane 6 for sealing the vent ring 5 is arranged in the vent ring, in order to improve the durability and the heat resistance of the ventilation filter membrane 6, the ventilation filter membrane 6 can still be used after repeated high-temperature and high-pressure sterilization, and the filter membrane 6 is an activated carbon fiber membrane. The material can resist high temperature and high pressure (up to 500 ℃), and can be recycled after being baked at 100 ℃.

For convenience of use, the outer side of the body 1 is provided with scale lines 7 and a frosted mark area 8, such as 5mL, 10mL, 15mL and the like. In order to reduce the manufacturing cost, the container body 1, the cover body 2 and the separating ring 10 are all made of polypropylene, and the material is not easy to crack, has good pressure resistance, can be sterilized at high temperature and high pressure, and has light weight and low price.

When the culture medium is used, the container body 1, the cover body 2 and the separating ring 10 are respectively sterilized, then the sterilized culture medium and the separating ring 10 are placed into the container body 1, and finally the inner side threads 3 of the cover body 2 are screwed into the outer side threads 4 of the bottle mouth of the container body 1. Because the 1cm wide vent ring 5 is arranged below the bottle mouth of the body 1 and the inside of the vent ring is provided with the air-permeable filter membrane 6, bacteria and dust in the air can be effectively adsorbed, and the external bacteria and dust are prevented from entering the container body 1 to pollute the tissue sample. The diameter and height of the container body 1 and the lid body 2 can be adjusted as required.

Finally, it is to be noted that the above-mentioned embodiments are only specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the invention should be considered as within the scope of the invention.

Claims

1. A plant tissue culture container comprises a container body and a cover body, and is characterized in that the container body is a cylindrical bottle with an open top surface, a plurality of concentric annular grooves are arranged on the inner bottom surface of the container body, and separating rings are arranged in the annular grooves; the cover body is arranged on the container body.

2. The container for plant tissue culture according to claim 1, wherein the top of the outer wall of the container body and the inner wall of the cover are provided with mating threads for threadedly coupling the container body and the cover.

3. The container for plant tissue culture according to claim 2, wherein a vent ring having a width of 1cm is provided on the container body below the screw thread, and an activated carbon fiber membrane is provided as a gas permeable filter membrane inside the vent ring.

4. A plant tissue culture container according to claim 1, wherein the height of said annular groove is 2 mm.

5. The plant tissue culture container according to claim 1, wherein the outer wall of the container body is further provided with volume scale marks and frosted mark areas.

6. The plant tissue culture container according to claim 1, wherein the container body has a volume of 0.1 to 1.0L.