CN211401879U - Boxed immunohistochemical pen - Google Patents
Boxed immunohistochemical pen Download PDFInfo
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- CN211401879U CN211401879U CN201921425326.3U CN201921425326U CN211401879U CN 211401879 U CN211401879 U CN 211401879U CN 201921425326 U CN201921425326 U CN 201921425326U CN 211401879 U CN211401879 U CN 211401879U
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- immunohistochemical pen
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Abstract
The utility model relates to an experimental apparatus technical field especially relates to a box-packed immunohistochemical pen. The boxed immunohistochemical pen comprises a box and a sealing film roll loaded in the box; the top cover of the box is provided with a rectangular opening for extracting the sealing film roll. Because the sealing film is waterproof and moisture-proof, has the advantages of strong corrosion resistance and no tearing after being repeatedly folded and used, can well optimize the existing immunohistochemical pen in the market, effectively reduce the use amount of antigen and antibody as far as possible, avoid the phenomena of drying and stripping caused by the loss of surface reagents, maintain the reagents on the glass slide to the maximum extent and reduce the experiment cost. Therefore, the product has the advantages of low production cost, long shelf life and difficult volatilization.
Description
Technical Field
The utility model relates to an experimental apparatus technical field especially relates to a box-packed immunohistochemical pen.
Background
Immunohistochemical technology is a method for analyzing the existence, location and semi-quantitative of corresponding antigens by specific binding and color development of antigens and antibodies. The kit has strong specificity, high sensitivity, rapidness, simplicity and convenience, thus becoming one of indispensable technical means in clinical pathological diagnosis and examination and providing important basis for precise medical treatment. An immunohistochemical pen is a marker pen with a special formula, and can form a thin film-shaped slightly green hydrophobic barrier by making a circle around a sliced sample. The barrier plays a role of hydrophobic isolation, can reduce the dosage of antibody and reagent, and avoids liquid flowing and diffusing during dyeing. In addition, when several different specimen sections are stained simultaneously on the same slide, the mixing between staining solutions can be prevented well. The immunohistochemical pen is suitable for various immunohistochemical staining experiments of pathological glass slides and has wide application.
However, the average price of the commercialized immunohistochemical pen in the prior art is more than 300 yuan, and the imported immunohistochemical pen also needs thousands of yuan, so that the cost performance is not high, and the popularization of each laboratory is difficult. Meanwhile, the shelf life of the commercialized immunohistochemical pen is only 2 years. Moreover, the existing commercial immunohistochemical pens are all made of volatile materials, are easy to dry and hard and cannot be normally used. In addition, the immunohistochemical pen is not easy to operate in a wet sheet, and if the quality of the immunohistochemical pen is poor, the composition materials of the immunohistochemical pen are likely to permeate into the reagent to influence the experimental result. Therefore, at present, people often use materials such as thin-head oil-based marking pens, lipsticks, paraffin, vaseline, neutral resin glue and the like to replace immunohistochemical pens, but generally, the water resistance is poor, and the phenomena of dry and peeling are caused, so that the experiment cannot be efficiently and smoothly completed.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a box-packed immunohistochemical pen to the weak point among the prior art, this box-packed immunohistochemical pen has low in production cost, and the shelf life is long and difficult volatile advantage.
In order to achieve the above purpose, the present invention is achieved by the following technical solutions.
The boxed immunohistochemical pen comprises a box and a sealing film roll loaded in the box; the top cover of the box is provided with a rectangular opening for extracting the sealing film roll.
The box is a waste box loaded with pathological glass slides.
The roll of sealing film is a Parafilm sealing film.
The length of the opening is 5.5cm, and the width is 0.6 cm.
The sealing film roll is a sealing film roll which is repeatedly folded into a rectangle.
The rectangle is 5.5cm in length and 2cm in width.
The thickness of the sealing film roll is 127 mu m.
The utility model has the advantages that:
(1) the utility model provides a boxed immunohistochemical pen, which comprises a box and a sealing film roll loaded in the box; the top cover of the box is provided with a rectangular opening for extracting the sealing film roll. Because the sealing film is waterproof and moisture-proof, has the advantages of strong corrosion resistance and no tearing after being repeatedly folded and used, can well optimize the existing immunohistochemical pen in the market, effectively reduce the use amount of antigen and antibody as far as possible, avoid the phenomena of drying and stripping caused by the loss of surface reagents, maintain the reagents on the glass slide to the maximum extent and reduce the experiment cost. And the quality guarantee period and the storage condition of the boxed immunohistochemical pen are not limited, thereby being convenient for forming commercialization in the market and particularly realizing the sharing of each common laboratory. And is suitable for immune staining experiments of conventional pathological glass slides such as immunohistochemistry, immunofluorescence, in situ hybridization and the like.
Drawings
Fig. 1 is a schematic structural diagram of a boxed immunohistochemical pen of the present invention in use.
Reference numerals:
a pathology slide 1 to be stained;
a sealing film 2;
the scratch edge region 3.
Detailed Description
In order to make the technical problem, technical solution and advantageous effects solved by the present invention more clearly understood, the following embodiments and drawings are combined to further explain the present invention in detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1.
The boxed immunohistochemical pen comprises a box and a sealing film roll loaded in the box; the top cover of the box is provided with a rectangular opening for extracting the sealing film roll.
Wherein, the box is a waste box loaded with pathological glass slides.
Among them, the roll of sealing film is preferably a Parafilm sealing film.
Wherein, the length of the opening is 5.5cm, and the width is 0.6 cm.
Wherein, the sealing film roll is a sealing film roll which is repeatedly folded into a rectangle.
Wherein the rectangle is 5.5cm in length and 2cm in width.
Wherein the thickness of the sealing film roll is 127 mu m.
The preparation method of the boxed immunohistochemical pen comprises the following steps:
step one, material preparation: preparing a sealing film roll, a box, a ruler, a knife, scissors, gummed paper and a marking pen;
measuring a rectangle with the length of 5.5cm and the width of 0.6cm in the middle of the top cover of the box by using a ruler, and marking by using a marking pen;
cutting along the rectangle which is just marked by using a knife;
removing the rectangles which are just separated to form an opening through which the sealing film roll can be drawn out;
cutting two long sealing films with the width of 5cm along the center line of the sealing film roll by using scissors;
sixthly, adhering the head and the tail of the two long sealing films together by using gummed paper;
step seven, repeatedly folding the long strip sealing film into a rectangle with the length of 5.5cm and the width of 2cm by the width of 2cm, namely the optimized immunohistochemical pen;
drawing a triangle with the long side of 2.2cm and the short side of 0.2cm at four corners of the folded sealing film by using a marking pen, and then cutting the triangle along the bevel edge by using scissors;
step nine, unfolding each sealing film and then repeatedly folding;
fixing one end of the folded long sealing film on a base in the box by using adhesive paper, drawing the other end of the folded long sealing film out of a rectangular opening of a top cover of the cut box, and then covering the box;
step eleven, taking the immunohistochemical pen formed by the sealing film at ordinary times, and only slightly tearing the pen.
The application method of the boxed immunohistochemical pen comprises the following steps:
step one, material preparation: preparing scissors, a sealing film, a plastic scraping blade and a pathological glass slide 1 to be dyed;
step two, tearing out a sealing film 2 from the boxed immunohistochemical pen;
step three, folding the sealing film 2 in half according to the size of the sliced specimen, drawing a rectangle with the area larger than or equal to half of the size of the specimen by using a marking pen, namely drawing the rectangle with the width unchanged and the length slightly larger than half of the length of the specimen, and cutting a small rectangle with the area larger than the area of the specimen by using scissors along the mark of the marking pen;
unfolding the cut sealing film 2, uncovering the transparent film part of the sealing film 2 from the paper part, and adhering the contact surface of the sealing film 2 to the pathological glass slide 1 to be dyed after being washed by PBS buffer solution;
fifthly, scraping several circles by using the smooth surface of the plastic scraper close to the edge of the sealing film 2 of the specimen to form a scraping edge area 3 so as to increase the contact surface of the sealing film 2 and the pathological glass slide 1 to be dyed to the maximum extent;
step six, dropwise adding a reagent to continue a next staining test of the pathological slide;
and seventhly, after the sealing film 2 is used, only slightly uncovering the sealing film 2.
It should be finally noted that the above embodiments are only intended to illustrate the technical solution of the present invention and not to limit the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced with other equivalents without departing from the spirit and scope of the technical solution of the present invention.
Claims (6)
1. The utility model provides a box-packed immunohistochemical pen which characterized in that: comprises a box and a sealing film roll loaded in the box; the top cover of the box is provided with a rectangular opening for extracting the sealing film roll; the roll of sealing film is a Parafilm sealing film.
2. The packaged immunohistochemical pen according to claim 1, wherein: the box is a waste box loaded with pathological glass slides.
3. The packaged immunohistochemical pen according to claim 1, wherein: the length of the opening is 5.5cm, and the width is 0.6 cm.
4. The packaged immunohistochemical pen according to claim 1, wherein: the sealing film roll is a sealing film roll which is repeatedly folded into a rectangle.
5. The packaged immunohistochemical pen according to claim 4, wherein: the rectangle is 5.5cm in length and 2cm in width.
6. The packaged immunohistochemical pen according to claim 1, wherein: the thickness of the sealing film roll is 127 mu m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921425326.3U CN211401879U (en) | 2019-08-28 | 2019-08-28 | Boxed immunohistochemical pen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921425326.3U CN211401879U (en) | 2019-08-28 | 2019-08-28 | Boxed immunohistochemical pen |
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| Publication Number | Publication Date |
|---|---|
| CN211401879U true CN211401879U (en) | 2020-09-01 |
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| CN201921425326.3U Active CN211401879U (en) | 2019-08-28 | 2019-08-28 | Boxed immunohistochemical pen |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441118A (en) * | 2019-08-28 | 2019-11-12 | 广东医科大学 | A kind of box-packed immunohistochemistry pen and its preparation method and application method |
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2019
- 2019-08-28 CN CN201921425326.3U patent/CN211401879U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441118A (en) * | 2019-08-28 | 2019-11-12 | 广东医科大学 | A kind of box-packed immunohistochemistry pen and its preparation method and application method |
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