CN206096147U - Immunity side direction chromatography detecting system - Google Patents
Immunity side direction chromatography detecting system Download PDFInfo
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- CN206096147U CN206096147U CN201621147634.0U CN201621147634U CN206096147U CN 206096147 U CN206096147 U CN 206096147U CN 201621147634 U CN201621147634 U CN 201621147634U CN 206096147 U CN206096147 U CN 206096147U
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Abstract
The utility model discloses an immunity side direction chromatography detecting system, include: the bottom plate, it is including being used for bearing the first offset plate of sample pads, nitrocellulose membranes with be used for bearing the second offset plate of pad of absorbing water, first offset plate and second offset plate respectively with nitrocellulose membranes's both ends fixed connection, nitrocellulose membranes on be provided with detect the area with matter the accuse take, the sample pad, it is fixed first offset plate on and with nitrocellulose membranes contact in order to guarantee to climb water, the pad that absorbs water, it is fixed the second offset plate on and with nitrocellulose membranes contact in order to guarantee to climb water. The utility model discloses a detecting system have with low costs, climb the effectual advantage of water.
Description
Technical field
The utility model is related to medical domain, and in particular to a kind of immune lateral chromatography detecting system.
Background technology
Immune lateral chromatography diagnostic techniques is adapted in various real-time test as a kind of stable and practical technology
Or onsite application (POCT).It is divided into by mark part and mainly have colloid gold immune lateral chromatography method, common fluorescent immunity laterally
Chromatography, time-resolved fluoroimmunoassay lateral chromatography method, on turn electrochemiluminescent immunoassay lateral chromatography method, the lateral layer of quantum dot fluorescence immunity
Analysis method etc., mainly has nitrocellulose filter, composite (fusion5), micro-fluidic etc. by coating part.
With the development of immunochromatography technique (immunochromatography) and colloidal gold technique, the especially nineties
Afterwards, diagnose the illness in vitro colloid gold immune lateral chromatography method (Gold immunochromatography assay, GICA) inspection
It is widely applied in survey.Quantitative determination is but carried out now, more and more higher is required to project precision and repeatability, pass through
The development in many years, nearest all kinds of fluorescence immunoassay lateral chromatography methods emerge in an endless stream.Main flow advanced technology is become by many public affairs
Department praises highly.
Current fluorescence immunoassay lateral chromatography method, typically using nitrocellulose filter as climbing water material, but at present
The length of nitrocellulose filter that adopts of detection means it is longer, it is bad to climb water effect, and offset plate is when reagent card is assembled, and needs
First nitrocellulose filter, sample pad, adsorptive pads are cut (5mm width) and then recomposition, packaging efficiency is low, and technique is loaded down with trivial details, because
Sample pad had been processed, and the order of sample pad both forward and reverse directions, fore-and-aft direction and sample pad is all random, is also manually put
The error of the position of sample pad can all affect precision when finally detecting.
Utility model content
The purpose of this utility model is to provide a kind of immune lateral chromatography detecting system, can effectively reduce cellulose nitrate
The length of plain film, reduces detection time, improves the degree of accuracy of detection, has saved cost.
The purpose of this utility model is achieved by the following technical solution：
The utility model provides a kind of immune lateral chromatography detecting system, including：
Base plate, it includes the first offset plate for carrying sample pad, nitrocellulose filter and for carrying adsorptive pads
Two offset plates；Described the first offset plate and the second offset plate is fixedly connected respectively with the two ends of described nitrocellulose filter；Described
Detection band and quality control band are provided with nitrocellulose filter；
Sample pad, it is fixed on the first described offset plate and with described nitrocellulose filter and contacts to ensure to climb water；
Adsorptive pads, it is fixed on the second described offset plate and with described nitrocellulose filter and contacts to ensure to climb water.
Further, also including jam, it includes：
Kerve, it is used for fixed described base plate；
Upper lid, is provided with the well for being loaded in described sample pad；
Detection window, it is used to detecting the detection window of described detection band and quality control band；Described detection window is arranged on kerve
Or on upper lid.
Further, described baseplate width is 4-6mm, and length is 6.5-7.5cm.
Further, the length of the first described offset plate is 1.8-2.0cm；The length of the second described offset plate is 1.5-
1.7cm；The length of described sample pad is 1.7-1.8cm；The length of described adsorptive pads is 1.2-1.4cm.
The utility model additionally provides a kind of preparation method of immune lateral chromatography detecting system, comprises the steps：
(1) detection band and quality control band are coated with nitrocellulose filter, by the both sides of nitrocellulose filter respectively with offset plate A
It is fixedly connected with offset plate B, described detection band is located between offset plate A and quality control band；
(2) sample pad is fixed on described offset plate A and with described nitrocellulose filter and contacts to ensure to climb water, will
Adsorptive pads are fixed on described offset plate B and with described nitrocellulose filter and contact to ensure to climb water；Obtain detecting system hair
Base；
(3) described detecting system blank is cut into into required size and obtains final product described immune lateral chromatography detecting system.
Further, described detecting system blank is cut into after required size and is also comprised the steps：
Described detecting system blank is cut into after required size and is put in jam, described jam includes：
Kerve, it is used for fixed described base plate；The kerve bottom is provided with for detecting described detection band and Quality Control
The detection window of band；
Upper lid, is provided with the well for being loaded in described sample pad.
Further, described step (2) specifically includes following steps：
Have fixed described base plate with " work " pattern, described sample pad is pasted onto on offset plate A and is pushed down described nitre
Acid cellulose film, pushes down width for 1-3mm；Described adsorptive pads are pasted onto on offset plate B and are pushed down described celluloid
Film, pushes down width for 1-3mm.
Further, described offset plate A width is 1.8-2.0cm, and the width of described nitrocellulose filter is 3.3-
The width of 3.8cm, described offset plate B is 1.5-1.7cm.
Further, described required size is that width is 4-6mm.
Compared with prior art, the utility model application at least possesses following beneficial effect：
Offset plate will be arranged to below sample pad and adsorptive pads, the length for reducing nitrocellulose filter (is reduced to original
Come 1/2nd of length or so), because the surface of offset plate is similar to minute surface, its resistance very little to climbing water, sample pad and suction
Water cushion aperture is very big, the resistance very little to climbing water, and compared with nitrocellulose filter, it might even be possible to ignore, this just drops significantly
The low distance for climbing water, so as to reduce the water time is climbed, and improves the accuracy of testing result.Wedge-shaped card adds after offset plate, than
In the case that overall product length is constant, celluloid film length reduces to 3.5cm, nitrocellulose filter cost by original 7cm
It is 10 times or so of offset plate, cost of material can be reduced.
Wedge-shaped card, when reagent card is assembled, needs first nitrocellulose filter, sample pad, adsorptive pads to be cut (5mm without offset plate
It is wide) and then recomposition, packaging efficiency is low, and technique is loaded down with trivial details；And new system is added after offset plate, can be when kilocalorie be assembled (before cutting out)
Sample pad, adsorptive pads are posted, is then cut again, packaging efficiency is greatly improved, cost of labor can be reduced, because of sample pad
Processed, the order of sample pad both forward and reverse directions, fore-and-aft direction and sample pad is all random, the position of also artificial setting-out product pad
The error put can all affect precision when finally detecting；And new system is added after offset plate, can be in kilocalorie sample pad, suction
Water cushion is posted, and is then cut again, sample pad both forward and reverse directions, fore-and-aft direction and the order of sample pad determined, while making
The site error of sample pad is less, reduces detection CV, improves system precision and repeatability.
Description of the drawings
Fig. 1 is the structural representation of existing detection card in the utility model immunity lateral chromatography detecting system embodiment；
Fig. 2 is a kind of structural representation of detection card in the utility model immunity lateral chromatography detecting system embodiment；
Fig. 3 is a kind of internal structure schematic diagram of kerve in the utility model immunity lateral chromatography detecting system embodiment；
Fig. 4 is a kind of structural representation of upper lid in the utility model immunity lateral chromatography detecting system embodiment.
Specific embodiment
For convenience of skilled artisan understands that technical solutions of the utility model, below in conjunction with the accompanying drawings and preferred embodiment pair
The technical solution of the utility model is further elaborated, it will be appreciated that preferred embodiment is the understanding of this programme for convenience, and
Not as restriction of the present utility model.
The detection card of existing immune lateral chromatography system is as shown in figure 1, it includes nitrocellulose filter 1, the He of sample pad 2
Adsorptive pads 3, sample pad 2 and adsorptive pads 3 are separately positioned on the two ends of nitrocellulose filter 1, and sample pad 2 and adsorptive pads 3 are all whole
It is pressed on nitrocellulose filter 1, carries out climbing water by the capillarity of nitrocellulose filter 1, on nitrocellulose filter 1 successively
Detection band 4 and quality control band 5 are provided with, are respectively used to detect and verify testing result.
The utility model scheme immune lateral chromatography detecting system as shown in Fig. 2 it includes nitrocellulose filter 8,
Nitrocellulose filter two ends are connected with offset plate, and wherein one end is the first offset plate 6 for carrying sample pad 2, and the other end is use
In the second offset plate 7 for carrying adsorptive pads 3, sample pad 2 and adsorptive pads 3 are separately fixed on corresponding offset plate, in celluloid
Detection band 4 and quality control band 5 are provided with film 8, are respectively used to detect and verify testing result.
Compared with existing detection card, detection card two ends of the present utility model replace part celluloid using offset plate
Film, shortening is climbed the water time, reduces the cost of detection card.
It will be clear that the utility model scheme is not limited the sequence of positions of detection band 4 and quality control band 5, root
Suitable order and position are selected according to needs.
Embodiment 1
The preparation method of the immune lateral chromatography detecting system that the utility model is provided is as follows：
Stick lower offset plate：
Nitrocellulose filter specification：23*3.5cm,
Offset plate A specifications：23* (1.9+0.2) cm, having at 2mm in side has a tangent line；
Offset plate B specifications：23* (1.6+0.2) cm, having at 2mm in side has a tangent line；
Note：Offset plate surface carries double faced adhesive tape, and the tangent line of its both sides is to cut double faced adhesive tape, it is convenient subsequently and nitrocellulose filter
Connection.
Two blocks of offset plates are attached to respectively nitre by the 2mm that tears respectively on the outside of two pieces of offset plate tangent lines wide double faced adhesive tape outside paster
The both sides of acid cellulose film, do not tear double faced adhesive tape is partially located in nitrocellulose filter outside, and it is fine that the part torn is pressed in nitric acid
On the plain film of dimension.
Patch sample pad：
The nitrocellulose filter of offset plate will be posted, according to the locality for being indicated, adsorptive pads end is put into what is made upper
In " work " shaped mold.Guarantee upper edge and the mould alignment of backboard, it is to avoid leave gap, push down nitric acid with " work " font fixture fine
The plain film of dimension and by its clamping in a mold (one side of non cohesive gel plate is upper), then tear sample pad location and (remove 2mm on offset plate A
Part beyond wide little bar) double faced adhesive tape (specification：23*1.9cm).Be careful not to the adsorptive pads of the mould of " work " font and
Sample pad end puts back.
By the sample pad for cutting (wide 1.75cm), along " work " shaped mold close coated film side start from one end it is right
Together (sample pad pushes down nitrocellulose filter 2mm), sample pad is pressed lightly on and is attached on offset plate to the other end successively.
Gently it is placed in the sample pad for having posted with the double faced adhesive tape paster torn before again, right-handed forefinger is again from a left side
Press one time to the right, it is ensured that sample pad smooth and pasted completely, then take down double faced adhesive tape paster.
By the adsorptive pads for cutting (wide 1.3cm) according to patch sample pad the step of be attached on offset plate B, by the profiled sheeting for posting from
Take out in mould.The unnecessary sample pad in profiled sheeting both sides and adsorptive pads are cut with scissors.
Slitting
The above-mentioned big plate for having posted is cut into into required width detection card, generally 5mm width.The structural representation of detection card
Figure is as shown in Figure 2 (structure is for example aforementioned).
Group card
Plastics jam is introduced
Plastics jam is made up of upper lid and bottom lid two parts, and Fig. 3 is the structural representation of kerve, and Fig. 4 is the structure of upper lid
Schematic diagram；As shown in Fig. 2 kerve includes fixing hole 11, it is used to fix kerve with upper lid, detects card standing groove, and it is by solid
The stopper slot 10 at fixed board 9 and two ends is surrounded, for placing detection card；In the middle part of detection card standing groove and nitrocellulose filter 8
Corresponding position is provided with detection window 12, and it is used to collect the testing result of detection band 4 and quality control band 5.
Bottom land is confirmed before being installed without dirty, completely without incompleteness, especially detects that the fixed plate 9 of card standing groove both sides is complete,
Can normally use.
Confirm that detection card is complete, sample pad, adsorptive pads N/D, detection card invariably specification shape, film no marking, without hair
Side, face are without signature pen trace etc..
Adsorptive pads one end is directed at into the site at the top of the jam of card bottom suction side, then by detection card compacting, it is ensured that detection
Card is all smooth to be placed in film groove, and the contact surface of bottom land is seamless, without rocking.
Buckle is covered beyond the Great Wall：
Cap sheathing mechanism as shown in figure 4, there is fixed column 13 inside, its with kerve on fixing hole 11 use cooperatively with by upper lid
It is fixed together with kerve；Sample holes 14 are provided with the position corresponding with sample pad 2, it is used to that sample to be added dropwise in sample pad 2
Product.
Cover whether complete in inspection, whether bar code has been sprayed on upper lid, whether there is breakage or incompleteness, it is ensured that above cover intact, can be with
Normally use.
Be placed in the kerve for assembling is neat on levels operation table top, and check the position of detection card whether accurately,
Completely, test strips order should not put back.
Again by upper cover buckle on kerve (sample holes 14 of upper lid are consistent with the extreme direction of sample pad 2 of kerve), will be upper with hand
Lid is pushed down.
By the detecting system for assembling, number according to nitrocellulose filter, being put into respectively on case pressing machine carries out pressure shell.Press
Shell to be checked whether the upper and lower covers for checking jam press sternly.
Detecting system assembling, posting label is successively placed in the valve bag of corresponding numbering, loads appropriate drying
Agent, in placing into aluminium foil bag, and carries out mark, and with sealing machine sack is sealed, standby.
Embodiment 2
A kind of preparation method of immune lateral chromatography detecting system, comprises the steps：
Coated antibody：Human muscle hemoglobin (MYO) detection antibody 1 is diluted to into fixed concentration (2.0mg/ with coating buffer solution
Ml), contrast agents 1 (goat-anti chicken IgY) are diluted to fixed concentration (2.0mg/ml), using special coating equipment BIODOT companies
XYZ3060 by above-mentioned two liquid coating on Sai Duolisi nitrocellulose filters 140 (NC films), 37 DEG C of drying boxes are dried 4
Hour (respectively obtaining detection band and quality control band), it is standby.Coating buffer solution Jia 3% for the phosphate buffer (PBs) of 0.01Mol/l
Sucrose as protective agent.
Labelled antibody：Human muscle hemoglobin (MYO) detection antibody 2 and contrast agents 2 (chicken IgY) are used into latex fluorescence labeling,
It is stored in storing liquid, standby, (50mMol/l Tris, 0.5%BSA, pH7.8).
Label prepares：Above-mentioned labelled antibody is pressed into 5 microlitres of volumes of desired concn specking in testing tube, is dried standby
With.
It is prepared by sample pad：By sample treatment liquid specking in sample pad or on, drying for standby.Sample treatment liquid is：Wherein
Tris-CL for molar concentration, remaining is weight/mass percentage composition.Ju Ti Pei Fang is as follows：Tris-CL：50mMol/l, 0.5%
Casein, 0.5%BSA, 0.1%Tween-20,0.05%PEG, 0.1%Tween-80,0.05%PVP, 0.3% 2 hydration lemon
Lemon acid acid sodium, 2% sucrose.
Abbreviation：BSA：Bovine serum albumin(BSA), Casein：Casein, Tris-CL：Trishydroxymethylaminomethane hydrochloric acid, PEG：
Polyethylene glycol, Tween-20：Polysorbas20, Tween-80：Tween 80, PVP：Polyvinylpyrrolidone.
Assembling detection card：Will coating it is good needed for the nitrocellulose filter of reagent, offset plate and processed good sample pad and
Adsorptive pads are pasted together according to mould, and are cut into required width detection card, generally 5mm width, the detection card group sheared
It is attached in corresponding jam (Cassette)；(old system is first nitrocellulose filter and processed good sample pad and water suction
Pad all cuts, generally 5mm width, is then being assembled in corresponding jam (Cassette) in order) jam is in sample pad
There is well top, and there is observation window nitrocellulose filter lower section side, for data acquisition.With common suction nozzle pipette samples dilution
(sample diluting liquid is that many people point mix, i.e. one big bottle of sample diluting liquid of box product) is to the testing tube for having put fluorescent material
It is interior, then add sample, blow and beat repeatedly for several times, dissolve fluorescent material, and the immune response between measured object in sample.It is then added to jam
In well, data are read within 11 minutes.Or first add sample in the testing tube for having put fluorescent material, then add Sample Dilution
Liquid, blows and beats for several times repeatedly, dissolves fluorescent material, and the immune response between measured object in sample.It is then added in jam well,
Due to capillary action lateral chromatography forward, when 11 minutes data are read.
When human muscle hemoglobin (MYO) project is done, because nitrocellulose filter shortens, climb water and substantially accelerate, specifically look at
Following table：
Explain：It is high 10 seconds that this method climbs water extraction.
Embodiment 3
Coated antibody：Troponin (cTnI) detection antibody 1 is diluted to into 3.0mg/ml concentration with coating buffer solution, is compareed
Reagent 1 (goat-anti chicken IgY) is diluted to 3.0mg/ml concentration, will be upper using the XYZ3060 of special coating equipment BIODOT companies
Two liquid coatings are stated on Sai Duolisi nitrocellulose filters (NC), 37 DEG C of drying boxes are dried 4 hours, standby.Coating buffering
Liquid for 0.01Mol/l phosphate buffer (PBs) Jia 3% sucrose as protective agent.
Labelled antibody：Troponin (cTnI) detection antibody 2 and contrast agents 2 (chicken IgY) are used into latex fluorescence labeling, is stored up
Exist in storing liquid, standby, (50mM Tris, 0.5%BSA, pH 7.8).
Label prepares：Above-mentioned labelled antibody is pressed into 5 microlitres of volumes of desired concn specking in suction nozzle inwall, is dried
It is standby.
It is prepared by sample pad：By sample treatment liquid press desired concn immersion or specking in sample pad or on, drying for standby.One
The formula of individual optimization is：Wherein Tris-CL for molar concentration, remaining is mass percent.Ju Ti Pei Fang is as follows：50mMol/l
Tris-CL, 0.5%Casein, 0.5%BSA, 0.1%Tween-20,0.05%PEG, 0.1%Tween-80,0.05%PVP,
0.3% 2 citric acid monohydrate acid sodium, 2% sucrose.
Abbreviation：BSA：Bovine serum albumin(BSA), Casein：Casein, Tris-CL：Trishydroxymethylaminomethane hydrochloric acid, PEG：
Polyethylene glycol, Tween-20：Polysorbas20, Tween-80：Tween 80, PVP：Polyvinylpyrrolidone.
Assembling detection card：New system will be coated with the nitrocellulose filter and offset plate and processed good sample of good required reagent
Pad and adsorptive pads are pasted together according to mould, and are cut into required width detection card, generally 5mm width, the inspection sheared
Survey card to be assembled in corresponding jam (Cassette)；Old system first nitrocellulose filter and processed good sample pad and
Adsorptive pads all cut, generally 5mm width, are then assembled in corresponding jam (Cassette) in order.Jam is in sample
There is well product pad top, and there is observation window nitrocellulose filter top, for data acquisition.With with label fluorescent material
Suction nozzle pipette samples are blown and beaten for several times repeatedly in reaction buffer bottle, dissolve fluorescent material, and between measured object in sample
Immune response.It is then added in jam well, due to capillary action lateral chromatography forward,
Data are read when 19 minutes.
When troponin (cTnI) project is done, body series precision is significantly improved, and specifically looks at following table：
Explain：Either fluorescent value or detectable concentration, this method coefficient of variation (CV) is at least enhanced about more than once, i.e., this
Method precision improves significantly.
The not most part of the utility model, those skilled in the art can as needed select existing technological means
Into, such as, and the particular location of detection band and quality control band, the particular location of well etc., will not be described here.
The above, specific embodiment only of the present utility model, but protection domain of the present utility model do not limit to
In this, any those familiar with the art can readily occur in change in the technical scope that the utility model is disclosed
Or replace, all should cover within protection domain of the present utility model.Therefore, protection domain of the present utility model should be with the power
The protection domain that profit is required is defined.
Claims (4)
1. a kind of immune lateral chromatography detecting system, it is characterised in that include：
Base plate, it includes the first offset plate for carrying sample pad, nitrocellulose filter and the second glue for carrying adsorptive pads
Plate；Described the first offset plate and the second offset plate is fixedly connected respectively with the two ends of described nitrocellulose filter；Described nitric acid
Detection band and quality control band are provided with cellulose membrane；
Sample pad, it is fixed on the first described offset plate and with described nitrocellulose filter and contacts to ensure to climb water；
Adsorptive pads, it is fixed on the second described offset plate and with described nitrocellulose filter and contacts to ensure to climb water.
2. immune lateral chromatography detecting system according to claim 1, it is characterised in that also including jam, it includes：
Kerve, it is used for fixed described base plate；
Upper lid, is provided with the well for being loaded in described sample pad；
Detection window, it is used to detecting the detection window of described detection band and quality control band；Described detection window be arranged on kerve or on
Cover.
3. immune lateral chromatography detecting system according to claim 1, it is characterised in that described baseplate width is 4-
6mm, length is 6.5-7.5cm.
4. immune lateral chromatography detecting system according to claim 3, it is characterised in that the length of the first described offset plate
For 1.8-2.0cm；The length of the second described offset plate is 1.5-1.7cm；The length of described sample pad is 1.7-1.8cm；Institute
The length of the adsorptive pads stated is 1.2-1.4cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621147634.0U CN206096147U (en) | 2016-10-21 | 2016-10-21 | Immunity side direction chromatography detecting system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621147634.0U CN206096147U (en) | 2016-10-21 | 2016-10-21 | Immunity side direction chromatography detecting system |
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| Publication Number | Publication Date |
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| CN206096147U true CN206096147U (en) | 2017-04-12 |
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ID=58485003
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370849A (en) * | 2016-10-21 | 2017-02-01 | 北京康思润业生物技术有限公司 | Immune-lateral-chromatography detecting system and preparing method thereof |
| CN109521207A (en) * | 2018-12-28 | 2019-03-26 | 广州菲康生物技术有限公司 | A kind of IGF-1 fluorescence immune chromatography detection kit |
-
2016
- 2016-10-21 CN CN201621147634.0U patent/CN206096147U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370849A (en) * | 2016-10-21 | 2017-02-01 | 北京康思润业生物技术有限公司 | Immune-lateral-chromatography detecting system and preparing method thereof |
| CN109521207A (en) * | 2018-12-28 | 2019-03-26 | 广州菲康生物技术有限公司 | A kind of IGF-1 fluorescence immune chromatography detection kit |
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