CN205426928U - Half quantitative determination kit - Google Patents
Half quantitative determination kit Download PDFInfo
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- CN205426928U CN205426928U CN201620268093.0U CN201620268093U CN205426928U CN 205426928 U CN205426928 U CN 205426928U CN 201620268093 U CN201620268093 U CN 201620268093U CN 205426928 U CN205426928 U CN 205426928U
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Abstract
The utility model provides a half quantitative determination kit, tie (3) and color comparison snap ring (4) including colloidal gold immunoassay chromatography test paper strip (1), box body (2), lid, color comparison snap ring (4) surround in box body (2) surface to can be the axle center with the axis of box body (2) rotates for box body (2), the surface box body (2) including hole (21), opening part (22) that box body (2) diapire was seted up that observation window (23), box body (2) roof were as a result seted up, test paper strip (1) place the box body in, wherein one end is tied (3) with the lid and is connected, the other end lies in near opening part (22), test paper strip (1) stretch out and return box body (2) by opening part (22), the lid tie (3) and stretch out and return box body (2) by hole (21), the kit when (3) atress is tied to the lid to hole (21) the motion stop gear (5).
Description
Technical field
This utility model relates to colloid gold immune detection field, in particular to a kind of half-quantitative detection test kit.
Background technology
The main biological significance of IGFBP-1 is transport and regulation insulin-like growth factor-i is combined with receptor.Recent study finds, after IGFBP-1 and gestation, parent and embryo growth and development etc. have substantial connection.
Rupture of membranes before just before giving birth is referred to as premature rupture of fetal membrane, is a kind of common obstetric complication, and its incidence rate accounts for the 2%～25% of childbirth sum.As do not diagnosed in time and process, female youngster's life can be jeopardized.Recent study finds, the diagnosable premature rupture of fetal membrane of biochemical marker in detection cervicovaginal liquid, there are some researches show, IGFBP-1 is as the index of Diagnosis of Premature Rupture, there is higher Sensitivity and Specificity, be an effective inspection method.
Meanwhile, IGFBP-1 and gestational diabetes occurrence risk are negative correlation.
Therefore the concentration of IGFBP-1 needs strict control.
The IGFBP-1 detection kit used at present can only carry out qualitative detection to IGFBP-1 in amniotic fluid, and not for IGFBP-1 immue quantitative detection reagent box of content in amniotic fluid.
And the existing test kit utilizing colloidal gold immunity chromatography to carry out half-quantitative detection, colorimetric card is fixed, it is not easy to the colored intensity with testing result compare, and need to detect again after well is loaded when being loaded, experiment flow is complicated, need the operation of professional, if operational error occurs, by loss sample and pollute experimental apparatus.
Utility model content
In view of this, this utility model provides a kind of half-quantitative detection test kit, and described test kit includes colloidal gold immuno-chromatography test paper strip, box body, lid bolt and colorimetric snap ring；Described colorimetric snap ring is surrounded on cartridge outer surface, and can rotate relative to box body with the axis of box body for axle center；；Described box body includes the opening part that hole that result watch window, box body roof offer, box portion bottom wall are offered；Described test strips is placed in box body, and wherein one end tethers with lid and connects, and the other end is positioned near described opening part；Described test strips is stretched out by opening part and returns box body；Described lid bolt is stretched out by hole and returns box body；Described test kit includes the position-limit mechanism moved during lid bolt stress to described hole.
Preferably, described lid bolt includes key and bolt cap；Described position-limit mechanism is removable endless member；Described removable endless member is buckled between the roof of bolt cap and described box body.
Preferably, described removable endless member is the side snap ring with opening.
Preferably, described removable endless member is the draw ring being connected on described bolt cap to remove.
Preferably, the opening part of described box body is provided with the seal closure matching with it and can be removed.
Preferably, sample-adding pad that the test strips of described colloidal gold immunochromatographimethod includes being sequentially connected with, conjugate pad, coated film, adsorptive pads；Containing gold colloidal glucagon like growth factor associated proteins I monoclonal antibody cross-linking agent on described conjugate pad；The coated film of described Test paper along deviate from conjugate pad direction be sequentially provided with one detection line T with together with nature controlling line C；Described detection line T is coated with immobilised glucagon like growth factor associated proteins I monoclonal antibody, and described nature controlling line C is coated with immobilization sheep anti mouse polyclonal antibody.
Preferably, described sample-adding pad has the sample-adding clinch being overlapped on described conjugate pad, and described conjugate pad has the conjugate clinch being overlapped on described coated film, and described adsorptive pads has the water suction clinch being overlapped on coated film.
Preferably, described sample-adding clinch leaves spacing with described conjugate clinch in their extension direction.
Preferably, the conjugate pad that described conjugate pad is made up of polyester cellulose film or glass fibre membrane.
Preferably, the coated film that described coated film is made up of nitrocellulose filter.
Compared with prior art, use the half-quantitative detection test kit that this utility model provides by colloid gold chromatographic test paper strip is inserted directly in sample sampling, utilize colloidal gold immunity chromatography that determinand is quickly detected.It is not required in prior art utilize liquid-transfering gun or the process of colloid dropper sampling, shortens the time needed for experiment flow.Avoiding because of operational error in sampling process, sample drops to, on laboratory table or other experimental articles, instrument, pollute.It also avoid because of sample loss, and need the situation of repeated sampling.
Further, contain with box body axis as axle center due to test kit, it is possible to the colorimetric snap ring rotated relative to box body, testing result can contrast by more convenient and on colorimetric snap ring colour band, it is judged that colored intensity.The half-quantitative detection test kit provided due to this utility model decreases sampling process, shortens experiment flow, and sample can carry out the contrast of colour developing result within the shorter time, it is simple to obtain testing result the most accurately.
Further, owing to the test strips in test kit can move up and down relative to the box body of test kit, test strips is extended and returns box body, it is to avoid the pollution of test strips and damage, the perfect test kit defencive function for test strips.
It can thus be seen that compared with existing detection device, above improvement obtains: save experiment flow required time, it is to avoid the pollution being likely to occur in experimentation and repeated sampling, detection process is the easiest, quick, accurate.
Accompanying drawing explanation
Fig. 1 is the reagent cartridge configuration schematic diagram that this utility model provides；
Fig. 2 is the structural representation of the test strips that this utility model provides；
Fig. 3 be the test strips result of determination that this utility model provides be schematic diagram time positive；
Fig. 4 be the test strips result of determination that this utility model provides be schematic diagram time negative；
Fig. 5 is the test strips result of determination that provides of this utility model schematic diagram when being invalid.
Wherein, test strips 1, box body 2, lid bolt 3, colorimetric snap ring 4, position-limit mechanism 5, seal closure 6, it is loaded pad 11, conjugate pad 12, coated film 13, adsorptive pads 14, detects line T, nature controlling line C, hole 21, opening part 22, result observation panel 23, key 31, bolt cap 32.
Detailed description of the invention
Claim of the present utility model is described in further detail by the mode below in conjunction with specific embodiment, but it is not intended that any restriction of the present utility model, the amendment of anyone limited number of time made in this utility model right, still within right of the present utility model.
For the ease of understanding this utility model, close embodiment below and further illustrate the technical solution of the utility model.
The schematic diagram of the box body 2 of the half-quantitative detection test kit provided for this utility model refering to Fig. 1 to Fig. 2, Fig. 1, Fig. 2 is the schematic diagram of test strips 1.
This experiment is novel provides a kind of half-quantitative detection test kit, and described test kit includes colloidal gold immuno-chromatography test paper strip 1, box body 2, lid bolt 3 and colorimetric snap ring 4；Described colorimetric snap ring 4 is surrounded on box body 2 outer surface, and can rotate relative to box body 2 with the axis of box body 2 for axle center；Described box body 2 includes the opening part 22 that hole 21 that result watch window 23, box body 2 roof offer, box body 2 diapire are offered；Described test strips 1 is placed in box body, and wherein one end is connected with lid bolt 3, and the other end is positioned near described opening part 22；Described test strips 1 is stretched out by opening part 22 and returns box body 2；Described lid bolt 3 is stretched out by hole 21 and returns box body 2；Described test kit includes the position-limit mechanism 5 moved during lid bolt 3 stress to described hole 21.
Above-mentioned, colorimetric snap ring 4 can be with the axis of box body 2 as axis rotation, it is simple to comparison and detection result, and the testing result therefore drawn is the most accurate.
Box body 2 two ends are equipped with opening, are to be opened in the hole 21 of box body 2 roof and be opened in the opening part 22 of box portion bottom wall respectively；The opening at box body two ends is easy to test strips 1 and lid bolt 3 stretches out and returns box body 2；
Test strips 1 one end is connected with lid bolt 3, and lid bolt 3 is stretched out by the hole 21 of box body roof along the direction of test strips 1 and returns box body 2；The other end of test strips 1 is near box body 2 diapire opening part 22, when lid bolt 3 is by the power along test strips 1 direction, and lid bolt 3 is returned in box body 2 by hole 21, and test strips 1 is stretched out box body 2 along Impact direction by opening part 22 simultaneously；
It is understood that when the opening part 22 of box body 2 exposes test strips 1, i.e. sampling.During detection, the test strips 1 stretching out box body 2 is inserted in sample, utilize colloidal gold immunity chromatography, sample will move along test strips 1 to the direction of lid bolt 3, and finally obtain testing result, i.e. can be observed, by result watch window 23, the situation that gold colloidal develops the color, colour developing situation is contrasted with colorimetric snap ring 4, it is judged that the colored intensity of testing result.
It is to be appreciated that before for avoiding sampling, test strips 1 stretches out box body 2, being contaminated or lose, causing testing result inaccurate, so being provided with position-limit mechanism 5.Position-limit mechanism 5 moves to the direction, hole 22 of box body 2 when can limit lid bolt 3 stress；Thus limit the test strips 1 being attached thereto and stretch out box body 2.
In this utility model embodiment, described lid bolt 3 includes key 31 and bolt cap (32)；Described position-limit mechanism 5 is removable endless member；Described removable endless member is buckled between the roof of bolt cap and described box body.
Above-mentioned, position-limit mechanism 5 is the endless member between the roof being buckled in bolt cap 32 and described box body 2.That is, before detection, endless member moves to the direction, hole 22 of box body 2 when can limit bolt cap 32 stress；Thus limit test strips 1 and stretch out box body 2.During detection, after removing endless member, move to the direction, hole 22 of box body 2 during bolt cap 32 stress；Test strips 1 stretches out box body 2, samples.
In this utility model embodiment, described removable endless member is the side snap ring with opening.
Seeing the schematic diagram that Fig. 1 is snap ring, endless member is the snap ring of one end open, this opening part, when removing snap ring, snap ring stress generation elastic deformation to opening part can be by the key 31 of lid bolt 3.
In other embodiments of this utility model, described removable endless member is the draw ring being connected on described bolt cap 32 to remove.
Above-mentioned, the form of draw ring has multiple, meets and can remove and spacing function.Draw ring can be connected with box body 2 by connecting post, the draw ring button of one end after removing from box body 2, key 31 can move downward relative to box body 2, thus exposes sample-adding pad 11.
In this utility model embodiment, the opening part 22 of described box body 2 is provided with the seal closure 6 matching with it and can be removed.
Above-mentioned, seal closure 6 can be PVC seal closure, is placed at box opening 21, when needing sampling, can remove, it is also possible to be the seal closure of other materials such as aluminum film on box body 2.Perfect paper box box body 2 protects the not contaminated function of test strips 1.
In this utility model embodiment, sample-adding pad 11 that the test strips 1 of described colloidal gold immunochromatographimethod includes being sequentially connected with, conjugate pad 12, coated film 13, adsorptive pads 14；Containing gold colloidal glucagon like growth factor associated proteins I monoclonal antibody cross-linking agent on described conjugate pad 12；The coated film 13 of described Test paper along deviate from conjugate pad 12 direction be sequentially provided with one detection line T with together with nature controlling line C；Described detection line T is coated with immobilised glucagon like growth factor associated proteins I monoclonal antibody, and described nature controlling line C is coated with immobilization sheep anti mouse polyclonal antibody.
Above-mentioned, can be by the sample of liquid condition (such as blood, blood plasma and other humoral sample diluents etc.) it is loaded onto on sample-adding pad 11, the sample of this liquid condition can pass through water sorption, move along sample-adding pad 11 to the conjugate pad 12 being attached thereto, move to the coated film 13 being attached thereto further along conjugate pad 12 after touching conjugate pad 12, move to the adsorptive pads 14 overlapped therewith further along coated film 13 after touching coated film 13.
After sample adds on sample-adding pad 11, sample is split by the capillarity of different directions, moves to successively on conjugate pad 12 and the coated film 13 of each test strips 1 after shunting.When sample moves to conjugate pad 12, detection antibody in sample, such as people's antibody, can combine with the specific antibody of coated colloid gold label on conjugate pad 12, the sample then containing the material combining or mixing from knot thing conjunction pad 12 continues to move on coated film 13, and combine with coated specific antibody on coated film 13 and be trapped, so that the determinand in sample is detected.It should be understood that the coated liquid of specific antibody is coated on coated film 13, i.e. detection line T；Nature controlling line C is usually coated liquid coated sheep anti mouse polyclonal antibody or goat-anti rabbit polyclonal antibody.Suitable spacing should be left between detection line T and nature controlling line C, and be set to the region being parallel to each other, both differentiations of being more convenient for.
In this utility model embodiment, described sample-adding pad 11 has the sample-adding clinch being overlapped on described conjugate pad 12, described conjugate pad 12 has the conjugate clinch being overlapped on described coated film 13, and described adsorptive pads 14 has the water suction clinch being overlapped on coated film 13.
Above-mentioned, " overlap joint " refers to, the head and the tail of two adjacent assemblies partly overlap, and form the attachment structure that fluid sample can be allowed to move at overlapping.For example, it is possible to the head and the tail part at two adjacent assemblies forms overlay structure more closely by pressing operation so that sample can be moved to downstream components by this overlay structure from the afterbody of upstream component.Upstream component can overlap the top of downstream components, it is also possible to overlaps lower section.When detection, can be by the sample of liquid condition (such as blood, blood plasma and other body fluid diluents etc.) it is loaded onto on sample-adding pad 11, the sample of this liquid condition can pass through water sorption, move along sample-adding pad 11 to the conjugate pad 12 overlapped therewith, move to the coated film 13 overlapped therewith further along conjugate pad 12 after touching conjugate pad 12, move to the adsorptive pads 14 overlapped therewith further along coated film 13 after touching coated film 13.
In this utility model embodiment, described sample-adding clinch leaves spacing with described conjugate clinch in their extension direction.
Above-mentioned, it is commonly referred to chromatography effect by sample-adding pad 11 movements to adsorptive pads 14 direction, it can thus be appreciated that, further, described sample clinch leaves spacing with described conjugate clinch in their extension direction, so that after colloid gold label, specific antibody can leave sufficient binding time with the determined antigen in sample on conjugate pad, as testing sample exists specific antigen to be detected, then after containing colloid gold label, antigen-antibody colloidal gold composite at the conjugate pad of specific antibody, can be formed；Described conjugate clinch leaves spacing with described water suction clinch in their extension direction, so that above-mentioned antigen-antibody colloidal gold composite forms antibody-antigen-antibody colloidal gold composite on coated film, i.e. there is the specific binding of double antibodies sandwich in reserved sufficient binding time.
In this utility model embodiment, the conjugate pad 12 that described conjugate pad 12 is made up of polyester cellulose film or glass fibre membrane.
Above-mentioned, conjugate pad 12 can be made up of polyester cellulose film, and polyester cellulose film is better than, with the affinity of gold colloidal, the conjugate pad that other materials is made, and prevents gold colloidal from occurring to assemble or form precipitation.
The sample-adding pad that sample-adding pad 11 can be made up of polyester film, cellulosic filter paper or non-woven fabrics, preferably glass fiber sample pad；Glass fibre is good relative to other materials dehydration property, be not adhered sample.
In this utility model embodiment.The coated film 13 that described coated film 13 is made up of nitrocellulose filter.
Above-mentioned, that the preferred nitrocellulose filter of coated film 13 is made coated film, the proteopexy ability of nitrocellulose filter is better than the coated film that other materials is made, it is possible to preferably solidification is coated on protein thereon.
In addition present invention also provides the detection method of this half-quantitative detection test kit:
1) removing the position-limit mechanism 5 of half-quantitative detection test kit, test strips 1 stretches out box body 2；
2) sample water sorption by adsorptive pads 14, moves along sample-adding pad 11 to the conjugate pad 12 overlapped therewith,
If containing determinand in sample liquid, determinand can be combined formation antigen-antibody colloidal gold composite by gold colloidal anti-determinand monoclonal antibody cross-linking agent on conjugate pad 3, sample liquid containing described complex continuously moves on coated film 13, and at detection line T, coated anti-determinand monoclonal antibody is combined on coated film 13, form anti-determinand clonal antibody determinand anti-determinand Antibody Gold Au composite, there is red stripes in detection line T, also occurring red stripes at nature controlling line C, testing result is positive；
If there is not determinand in sample liquid, detection line T then redfree band occurs red stripes occur at only nature controlling line C, and testing result is negative；
If red stripes does not all occur in detection line T and nature controlling line C, then representative operation is wrong or testing result is invalid, need to repeat test.
Result judges: after being loaded 10 minutes, observed result window 23, and refering to Fig. 3, when nature controlling line C and detection line T has red stripes to occur containing the antibody can being combined with detection protein-specific in explanation sample, determinand is positive；
Refering to Fig. 4, when only nature controlling line C has red stripes to detect line T redfree band, illustrate that in sample, determinand is feminine gender.
All do not occur red stripes refering to Fig. 5 as nature controlling line C and detection line T, then representative operation is wrong or testing result is invalid, need to repeat test.
Testing result is contrasted with colorimetric card, it is judged that colour developing situation.
Additionally, present invention also provides the preparation method of colloidal gold immuno-chromatography test paper strip 1 in half-quantitative detection test kit: test strips 1 includes sample-adding pad 11, conjugate pad 12, coated film 13 and the adsorptive pads 14 being from left to right sequentially connected with；Containing gold colloidal anti-determinand monoclonal antibody cross-linking agent on the conjugate pad 12 of described Test paper；From left to right be sequentially provided with on the coated film 13 of described Test paper one detection line T with together with nature controlling line C；Described detection line T is coated with immobilised anti-determinand monoclonal antibody, and described nature controlling line C is coated with immobilization sheep anti mouse polyclonal antibody；
Wherein:
1) preparation method of sample-adding pad 11 is: sample-adding pad 11 is cut into the size of 20mm × 300mm, is immersed in sample pad buffer, take out, in drying at room temperature 16-18 hour after 1 hour.
The buffer formulation of sample-adding pad 11 is as follows: 2%BSA, 1%PVP, 0.5%Tween are dissolved in PBS (pH7.4) buffer of 0.01.
2) preparation method of conjugate pad 12:
1. the preparation of colloidal gold solution: be 1mg/ml chlorauric acid solution 950ml heated and boiled 5～10 minutes by concentration, adds the citric acid three sodium solution 50ml that concentration is 100mg/ml, mixing, continues heating 5～10 minutes, till solution went clear rose.Add purified water and supply volume to 1000ml.
The preparation of the most anti-determinand antibody gold label liquid: take above-mentioned colloidal gold solution 300ml, adds 0.1MK2CO3Being placed in and be stirred at room temperature, the pH value of regulation solution is to 8.0；Take anti-determinand monoclonal antibody 6mg, add concentration be 0.015M volume be that 20mlPBS buffer solution adds in the colloidal gold solution in stirring after mixing；To stir 2 hours under above-mentioned mixed solution room temperature；Stirring adds 3% PEG 20000 solution 160ml after terminating, mixing；With 13000 revs/min under room temperature, it is centrifuged and separates above-mentioned solution in 30 minutes；Abandoning supernatant after centrifugal end, takes precipitation, is preserved in liquid by this golden labeling antibody being precipitated and dissolved in 100ml.
3. after above-mentioned anti-determinand monoclonal antibody gold mark liquid gold labeling antibody being preserved liquid dilution, it is laid on conjugate pad 12, is dried 4～6 hours in being placed in the drying room that temperature is 37 DEG C；Sealing bag together put into by dried gold mark conjugate pad 12 with desiccant, puts in glass desicator and saves backup.
3) preparation method of coated film 13:
1. it is coated
It is coated the preparation of liquid: take boric acid 12g, sodium hydroxide 2g and be dissolved in purified water and be settled to 1000ml.
Detection line T: rule on coated film 13, one end marking pen at film carries out nature controlling line C and the labelling of detection line T, the distance of nature controlling line C and detection line T is 0.5cm, detection line T is 1cm with the distance of coated film 13 lower edge, anti-determinand monoclonal antibody is diluted to 1.2mg/mL it is coated with the above-mentioned liquid that is coated, line concentration is 1 μ L/cm, speed 100mm/s.
Nature controlling line C: sheep anti mouse polyclonal antibody is diluted to 1.2mg/mL and is coated by the above-mentioned liquid that is coated, line concentration is 1 μ L/cm, speed 100mm/s.
2. it is dried
It is placed in 37 DEG C of drying rooms 4～6 hours；Coated film 13 is together put into after Gan Zaoing sealing bag with desiccant be placed in glass desicator and save backup.
It addition, the assemble method of colloidal gold immunity chromatography test strips 1 is as follows:
1. utilizing guillotine to cut sample-adding pad 11, conjugate pad 12 and adsorptive pads 14, sample-adding pad 11 width after cutting is 10mm, a length of 58mm；It is 7mm that conjugate 12 after cutting pads width, a length of 58mm；Adsorptive pads 14 width after cutting is 10mm, a length of 58mm.
2. the adsorptive pads 14 that width is 10mm is mounted on PVC base 1; and make its lower end and coated film 13 laminate 2mm; then conjugate pad 12 lower end protecting film is torn off; the conjugate pad 12 of width 7mm is laminated 2mm with coated film 13, below conjugate pad 12, pastes the sample-adding pad 11 of width 10mm further；Need during stickup to advance absorbent paper, conjugate pad 3, sample-adding pad 11 roller uniform, slight, to add strong adhesive power, and prevent bubble.
3. taking out the Test paper pasted to be placed on lath cutter, being cut into width is 2mm, and the desiccant then putting into a bag 1g in aluminium foil bag and the Test paper cut, by aluminium foil bag heat sealing on sealing machine.
In this experiment new embodiment, detection device also can be containing not shown dropper, desiccant, description, color label, sample collection tube and sample diluting liquid.
Detection device provided herein uses colloidal gold immunity chromatography, utilize the tag recognition being positioned on adsorptive pads 5 label 6 and the watch window 8 offered of box body 7 match, improve the efficiency of Clinical detection, avoid owing to mark is not clear simultaneously, the waste of the detection device caused.
In this experiment new embodiment, detection device also can be containing not shown dropper, desiccant, description, color label, sample collection tube and sample diluting liquid.
Claims (10)
1. a half-quantitative detection test kit, it is characterised in that: described test kit includes colloidal gold immuno-chromatography test paper strip (1), box body (2), lid bolt (3) and colorimetric snap ring (4)；Described colorimetric snap ring (4) is surrounded on box body (2) outer surface, and can be that axle center rotates relative to box body (2) with the axis of box body (2)；Box body (2) described in surface includes the opening part (22) that hole (21) that result watch window (23), box body (2) roof offer, box body (2) diapire are offered；Described test strips (1) is placed in box body, and wherein one end is connected with lid bolt (3), and the other end is positioned near described opening part (22)；Described test strips (1) is stretched out by opening part (22) and returns box body (2)；Described lid bolt (3) is stretched out by hole (21) and returns box body (2)；The position-limit mechanism (5) moved to described hole (21) when described test kit includes lid bolt (3) stress.
2. half-quantitative detection test kit as claimed in claim 1, it is characterised in that: described lid bolt (3) includes key (31) and bolt cap (32)；Described position-limit mechanism (5) is removable endless member；Described removable endless member is buckled between the roof of bolt cap (32) and described box body (2).
3. half-quantitative detection test kit as claimed in claim 2, it is characterised in that: described removable endless member is the side snap ring with opening.
4. half-quantitative detection test kit as claimed in claim 2, it is characterised in that: described removable endless member is the draw ring being connected on described bolt cap (32) to remove.
5. half-quantitative detection test kit as claimed in claim 1, it is characterised in that: the opening part (22) of described box body (2) is provided with the seal closure (6) matching with it and can be removed.
6. half-quantitative detection test kit as claimed in claim 1, it is characterised in that: sample-adding pad (11) that the test strips (1) of described colloidal gold immunochromatographimethod includes being sequentially connected with, conjugate pad (12), coated film (13), adsorptive pads (14)；Described conjugate pad (12) is upper containing gold colloidal glucagon like growth factor associated proteins I monoclonal antibody cross-linking agent；The coated film (13) of described Test paper is sequentially provided with one detection line (T) and nature controlling line (C) together along deviating from conjugate pad (12) direction；Described detection line (T) is coated with immobilised glucagon like growth factor associated proteins I monoclonal antibody, and described nature controlling line (C) is coated with immobilization sheep anti mouse polyclonal antibody.
7. half-quantitative detection test kit as claimed in claim 6, it is characterized in that: described sample-adding pad (11) has the sample-adding clinch being overlapped on described conjugate pad (12), described conjugate pad (12) has the conjugate clinch being overlapped on described coated film (13), and described adsorptive pads (14) has the water suction clinch being overlapped on coated film (13).
8. half-quantitative detection test kit as claimed in claim 6, it is characterised in that: described sample-adding clinch leaves spacing with described conjugate clinch in their extension direction.
9. half-quantitative detection test kit as claimed in claim 6, it is characterised in that: the conjugate pad (12) that described conjugate pad (12) is made up of polyester cellulose film or glass fibre membrane.
10. half-quantitative detection test kit as claimed in claim 6, it is characterised in that: the coated film (13) that described coated film (13) is made up of nitrocellulose filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620268093.0U CN205426928U (en) | 2016-03-31 | 2016-03-31 | Half quantitative determination kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620268093.0U CN205426928U (en) | 2016-03-31 | 2016-03-31 | Half quantitative determination kit |
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| Publication Number | Publication Date |
|---|---|
| CN205426928U true CN205426928U (en) | 2016-08-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620268093.0U Expired - Fee Related CN205426928U (en) | 2016-03-31 | 2016-03-31 | Half quantitative determination kit |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107860908A (en) * | 2017-11-07 | 2018-03-30 | 兰赫(上海)生物科技有限公司 | Biochemical detection kit for detection and analysis |
| CN108439305A (en) * | 2018-04-27 | 2018-08-24 | 深圳华创生物医药科技有限公司 | A kind of ampoule bottle rotarily opens device and detection reagent packing box |
| CN110045124A (en) * | 2019-04-01 | 2019-07-23 | 芜湖森爱驰生物科技有限公司 | Interleukin-6 detection kit |
-
2016
- 2016-03-31 CN CN201620268093.0U patent/CN205426928U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107860908A (en) * | 2017-11-07 | 2018-03-30 | 兰赫(上海)生物科技有限公司 | Biochemical detection kit for detection and analysis |
| CN108439305A (en) * | 2018-04-27 | 2018-08-24 | 深圳华创生物医药科技有限公司 | A kind of ampoule bottle rotarily opens device and detection reagent packing box |
| CN110045124A (en) * | 2019-04-01 | 2019-07-23 | 芜湖森爱驰生物科技有限公司 | Interleukin-6 detection kit |
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