CN109521207A - A kind of IGF-1 fluorescence immune chromatography detection kit - Google Patents
A kind of IGF-1 fluorescence immune chromatography detection kit Download PDFInfo
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- CN109521207A CN109521207A CN201811620115.5A CN201811620115A CN109521207A CN 109521207 A CN109521207 A CN 109521207A CN 201811620115 A CN201811620115 A CN 201811620115A CN 109521207 A CN109521207 A CN 109521207A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention discloses a kind of IGF-1 fluorescence immune chromatography detection kits, and including detection casing clamping body set by test strips and test strips outside, the test strips are by backing bottom plate, and backing bottom plate is made of nitrocellulose filter, sample bonding pad and absorbing membrane；The invention also discloses a kind of preparation methods of IGF-1 fluorescence immune chromatography detection kit, and the preparation method is the following steps are included: S1: prepared by detection line (T line) and nature controlling line (C line)；S2: the preparation of bonding pad；S3: sample pad processing；S4: test strips assembling；S5: the assembling of test strips finished product and detection；The present invention uses the lanthanide series with unique fluorescent characteristic and its chelate as time-resolved fluorescence substance, avoid overlap problem existing for excitation and emission spectrum, and the fluorescence decay time of lanthanide series and its chelate is long, the features such as interference for excluding exciting light has high sensitivity, and high specificity, stability is good and "dead" pollution.
Description
Technical field
The present invention relates to time-resolved fluoroimmunoassay chromatographic technique field, specially a kind of IGF-1 fluorescence immune chromatography inspection
Test agent box.
Background technique
Immunoassay is the method using antigen and antibody response detection antigen or antibody.It is used in the prior art
Immunoassay is broadly divided into: radio immunoassay, enzyme-linked immunization, chemiluminescence immunoassay, chromatograph-mass spectrometer coupling
Technology.Wherein, radioactive immunoassay: be exactly using radioisotope labeling antigen or antibody, then with measured antibody
Or antigen binding, the principle of antigen antibody complex is formed to be analyzed；Enzyme-linked immunization: specificity with higher and spirit
Sensitivity, while can be applied to the detection of great amount of samples, it is widely used in clinical detection；Chemiluminescence immunoassay: it is
The product that chemiluminescence and immune response are combined is surveyed with a para-immunity of the direct labelled antibody of chemiluminescent agent or antigen
Determine method, chemiluminescence immunoassay has many advantages, such as that high sensitivity, easy to operate, analysis speed is fast and "dead"；
Chromatography-mass spectroscopy technology used in conjunction: be it is a kind of integrate efficiently separate the method qualitative, quantitative with multicomponent, to higher boiling, do not wave
The separation and identification of hair and heat-labile compound have unique advantage.
The prior art equally exists following deficiency:
1. radio immunoassay: poor repeatability, non-specific binding rate is high, and agents useful for same has certain radioactivity,
If human body Long Term Contact will cause certain injury；
2. enzyme-linked immunization: being difficult to analyze multiple components simultaneously, still will appear to the analysis detection of certain analogues
Cross reaction influences testing result, depends on the selection of reagent unduly, is not applied for the field quick detection of base；
3. chemiluminescence immunoassay:: in practical applications there are still deficiency, such as equipment instrument is excessive, practical sample
The interference of product matrix, the precision of small molecule detection are limited etc.；
4. chromatography-tandem mass spectrometry: instrumental method can accurately carry out quantitative analysis, but equipment it is expensive, it is complicated for operation,
Require sample purity that high, testing cost is high, can not execute-in-place, and professional is needed to operate；
For this purpose, the present invention proposes a kind of IGF-1 fluorescence immune chromatography detection kit and preparation method thereof to provide one kind
It is easy to operate, quick, the low-cost method of inspection.
Summary of the invention
The purpose of the present invention is to provide a kind of IGF-1 fluorescence immune chromatography detection kits and preparation method thereof, to mention
For a kind of easy to operate, quick, low-cost method of inspection.
To achieve the above object, the invention provides the following technical scheme: a kind of IGF-1 fluorescence immune chromatography detection reagent
Box including detection casing clamping body and detects test strips set in casing clamping body, and the main body of the test strips is by backing bottom plate, backing bottom
The sample bonding pad and absorbing membrane structure that nitrocellulose filter that plate upper end is bonded, nitrocellulose filter left and right ends are bonded
At；
The nitrocellulose filter upper end has been respectively arranged on the left side and the right side detection line and nature controlling line；
The sample bonding pad is made of the bonding pad that the sample pad of layer structure is bonded with sample pad side-lower.
Preferably, it is coated with the first monoclonal antibody of time-resolved fluorescence mass signatures on the bonding pad, and combines
It pads dry after surfactant buffer immersion treatment by glass fibre element film and obtains, the time-resolved fluorescence substance is lanthanum
Series elements substance.
Preferably, it is coated with another monoclonal antibody in the detection line, is coated with secondary antibody on the nature controlling line.
Preferably, the upper end of the detection casing clamping body is equipped with the test paper slot for mutually blocking the trough body structure matched with test strips, described
The side of test paper slot is equipped with the hinged groove of trough body structure, and fixation is inserted with the hinged of cylindrical structure in the groove body of hinged groove
Axis is hinged with the gland of plate structure in the outer circle of the articulated shaft.
Preferably, the lower end of the gland and the upper surface of test strips mutually press, the middle position of the gland upper surface
Place is equipped with the well of frame structure.
Preferably, the sample pad is that glass fibre element film is dried after surfactant buffer immersion treatment and obtained.
A kind of preparation method of IGF-1 fluorescence immune chromatography detection kit, the preparation method the following steps are included:
S1: prepared by detection line (T line) and nature controlling line (C line), first affix to nitrocellulose filter on backing bottom plate, uses
The anti-human IGF-1 monoclonal antibody of mouse is diluted to 0.5mg/mL by 15mmol/L pH7.4 phosphate buffer, is used to prepare T line；
Sheep anti-mouse antibody is diluted to 1mg/mL with 15mmol/L pH7.4 phosphate buffer, is used to prepare C line；Liquid is drawn by 1uL/cm
Amount is uniformly drawn the antibody after above two dilution to preparation T line and C line on nitrocellulose filter with film instrument is drawn；It will pull
Nitrocellulose filter be placed in 37 DEG C of drying boxes dry 3-4h；
S2: the preparation of bonding pad, the rabbit-anti people that time-resolved fluorescence substance latex beads will be marked with by drawing film instrument
IGF-1 antibody is sprayed on the polyester film that width is 6-9mm according to the flow of 6uL/cm, and bonding pad is prepared in 37 DEG C of dry 3-4h
S3: sample pad processing, after sample pad carries out closing in advance with the buffer immersion containing surfactant, 50 DEG C of dryings
Overnight, buffer herein are as follows: 100mM pH8.0Tris.Hcl buffer, wherein containing 2%BSA, 0.5%PEG 4000,
0.1% Tween-20 and 5% sucrose；
S4: sample pad obtained in abovementioned steps, fixed laminate of bonding pad are pasted onto nitrocellulose by test strips assembling
The nearly T line end of film, and water absorption pad is fixed and laminates the other end for being pasted onto nitrocellulose filter；
S5: the assembling of test strips finished product and detection, progress test strips finished product assembling first, by the good big paperboard item of column with cutting
Machine is cut by the width of every 2.5-4mm, and the test strips clamping after then cutting out is into test casing clamping body；Test strips
Detection, assembled test strips are detected with double-antibody method: sample to be tested about 100uL being taken to be added drop-wise in sample pad, to
Sample is blood sample and dilution liquid mixture, and the IGF-1 in sample is formed in conjunction with the monoclonal antibody marked on bonding pad
Antigen-labelled antibody compound continues to chromatograph when nitride layer to be composite is analysed to T line in conjunction with another immobilised antibody herein
Compound is formed with goat anti-rabbit antibody to C line, under the excitation of Time-resolved fluorescence assay instrument, labelled element shows
Fluorescence, the labelled antibody that T knot is closed is more, and fluorescence intensity is higher, and T line fluorescence intensity is directly proportional to the IGF-1 level in sample.
Compared with prior art, the beneficial effects of the present invention are:
1. it is different from traditional fluorescein label, using the lanthanide series and its chelate conduct with unique fluorescent characteristic
Time-resolved fluorescence substance, the Stokes displacement between lanthanide series exciting light and transmitting light is larger, avoids excitation and transmitting
Overlap problem existing for spectrum excludes the interference of exciting light, so that it is significantly different from background fluorescence by wavelength resolution mode；
2. and lanthanide series exciting light spectrum is wider, the optical filter for only allowing transmitting fluorescence to pass through can be used, thus into one
Step distinguishes specificity fluorescent and background fluorescence, eliminates interference；
It is the 10 of conventional fluorescent 3. the fluorescence decay time of lanthanide ion chelate is long3~106Times, it is poor by minute
High s/n ratio may be implemented；
4. it can receive excitation repeatedly simultaneously, to greatly improve the signal value that instrument detects, sample is effectively excluded
The features such as interference of natural fluorescence has high sensitivity, and high specificity, stability is good and "dead" pollution.
Detailed description of the invention
Fig. 1 structure of the invention axis side view；
Fig. 2 is structure of the invention half sectional view；
Fig. 3 is sample of the present invention test result and regression curve comparison diagram.
In figure: 1, detecting casing clamping body；2, test strips；3, gland；4, backing bottom plate；5, nitrocellulose filter；6, sample knot
Close pad；7, absorbing membrane；101, test paper slot；102, hinged groove；103, articulated shaft；301, well；501, detection line；502, Quality Control
Line；601, sample pad；602, bonding pad.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Fig. 1 to 3 is please referred to, the present invention provides a kind of technical solution: a kind of IGF-1 fluorescence immune chromatography detection kit,
Including detection casing clamping body 1 and detect test strips 2 set in casing clamping body 1, it is characterised in that；The main body of test strips 2 is by backing bottom
The sample that nitrocellulose filter 5 that plate 4,4 upper end of backing bottom plate are bonded, 5 left and right ends of nitrocellulose filter are bonded combines
Pad 6 and absorbing membrane 7 are constituted；
5 upper end of nitrocellulose filter has been respectively arranged on the left side and the right side detection line 501 and nature controlling line 502；
602 structure of bonding pad that sample bonding pad 6 is bonded by the sample pad 601 and 601 side-lower of sample pad of layer structure
At.
Further, the first monoclonal antibody of time-resolved fluorescence mass signatures is coated on bonding pad 602, it is single herein
Clonal antibody is the anti-IGF-1 monoclonal antibody of mouse, the IGF-1 and combination when sample to be tested is contacted with bonding pad 602, in sample
The monoclonal antibody marked on pad 602 combines, and forms antigen-labelled antibody compound, and bonding pad 602 is by glass fibre element
Film is dry after surfactant buffer immersion treatment and obtains, also can be by polyester film through surfactant here in connection with pad 602
It dries and obtains after buffer immersion treatment, and glass fibre element film and polyester film make sample pad and bonding pad all have good branch
Intensity, stable chemical inertness and good permeability are supportted, time-resolved fluorescence substance is lanthanide series substance, herein group of the lanthanides member
Plain substance is one of lanthanide series, the conjugate of lanthanide series and latex, the chelate of lanthanide series, and lanthanide series is
Europium, one of terbium, the fluorescence spectrum of lanthanide series have biggish Stokes to be displaced, will not phase between excitation spectrum and emission spectrum
Mutually overlapping, in addition the spectral signal peak of its transmitting is very narrow, fluorescence lifetime is long, can interfere to avoid background fluorescence, improve detection
Precision.
Further, nitrocellulose filter 5 is equipped with detection line 501 and nature controlling line 502, and detection line 501 is coated with another
Monoclonal antibody, nature controlling line 502 are coated with secondary antibody, when nitride layer to be composite is analysed to detection line 501 with it is immobilised herein
Antibody combines, and continues to form compound with goat anti-rabbit antibody at chromatography to nature controlling line 502.
Further, the upper end for detecting casing clamping body 1 is equipped with the test paper slot 101 for the trough body structure matched with 2 phase card of test strips, examination
The side of paper groove 101 is equipped with the hinged groove 102 of trough body structure, and fixation is inserted with cylindrical knot in the groove body of hinged groove 102
The articulated shaft 103 of structure, the gland 3 of plate structure is hinged in the outer circle of articulated shaft 103, and this structure facilitates consolidating for test strips 2
Fixed and test.
Further, the lower end of gland 3 and the upper surface of test strips 2 mutually press, and the middle position of 3 upper surface of gland is set
There is the well 301 of frame structure, makes test strips 2 by pressure labor in detection casing clamping body 1 by gland 3, and then entire test strips 2
Held stationary during test or movement.
Further, sample pad 601 is that glass fibre element film is dried after surfactant buffer immersion treatment and obtained,
Sample pad 601 also can be for polyester film is dried after surfactant buffer immersion treatment and obtains herein, and this set makes sample
When contacting with bonding pad 602, improve the IGF-1 activity in sample, make IGF-1 in sample can more fully with sample pad 601
In the monoclonal antibody that is marked combine.
A kind of preparation method of IGF-1 fluorescence immune chromatography detection kit, the preparation method the following steps are included:
S1: prepared by detection line (T line) and nature controlling line (C line), first affix to nitrocellulose filter on backing bottom plate, uses
The anti-human IGF-1 monoclonal antibody of mouse is diluted to 0.5mg/mL by 15mmol/L pH7.4 phosphate buffer, is used to prepare T line；
Sheep anti-mouse antibody is diluted to 1mg/mL with 15mmol/L pH7.4 phosphate buffer, is used to prepare C line；Liquid is drawn by 1uL/cm
Amount is uniformly drawn the antibody after above two dilution to preparation T line and C line on nitrocellulose filter with film instrument is drawn；It will pull
Nitrocellulose filter be placed in 37 DEG C of drying boxes dry 3-4h；
S2: the preparation of bonding pad, the rabbit-anti people that time-resolved fluorescence substance latex beads will be marked with by drawing film instrument
IGF-1 antibody is sprayed on glass fibre element film or polyester film that width is 6-9mm according to the flow of 6uL/cm, 37 DEG C of dry 3-4h
Bonding pad is prepared；Time-resolved fluorescence substance latex beads are latex beads containing europium, i.e., time-resolved fluorescence substance is lanthanum
The conjugate of series elements europium and latex；As needed, time-resolved fluorescence substance it is adjustable for lanthanide series, lanthanide series with
One of the conjugate of latex, chelate of lanthanide series；Lanthanide series can be one of europium, terbium.
S3: sample pad processing, after sample pad carries out closing in advance with the buffer immersion containing surfactant, 50 DEG C of dryings
Overnight, buffer herein are as follows: 100mM pH8.0Tris.Hcl buffer, wherein containing 2%BSA, 0.5%PEG 4000,
0.1% Tween-20 and 5% sucrose；
S4: sample pad obtained in abovementioned steps, bonding pad fixation are laminated on nitrocellulose filter by test strips assembling
Nearly T line end, and water absorption pad is fixed to the other end for being laminated on nitrocellulose filter；
S5: test strips detection is assembled with finished product, progress test strips finished product assembling first, by the good big paperboard item of column with cutting
Machine is cut by the width of every 2.5-4mm, and the test strips clamping after then cutting out is into test casing clamping body；Test strips
Detection, assembled test strips are detected with double-antibody method: sample to be tested about 100uL being taken to be added drop-wise in sample pad, to
Sample is blood sample and dilution liquid mixture, and the IGF-1 in sample is formed in conjunction with the monoclonal antibody marked on bonding pad
Antigen-labelled antibody compound continues to chromatograph when nitride layer to be composite is analysed to T line in conjunction with another immobilised antibody herein
Compound is formed with goat anti-rabbit antibody to C line, under the excitation of Time-resolved fluorescence assay instrument, labelled element shows
Fluorescence, the labelled antibody that T knot is closed is more, and fluorescence intensity is higher, and T line fluorescence intensity is directly proportional to the IGF-1 level in sample.
Standard curve making: pattern detection takes IGF-1 calibration object a set of.Each 100uL of each calibration object is taken, detection is added to
In well 301 on casing clamping body 1, after 10 minutes, luminous value is detected with time-resolved fluorescence quantitative analysis instrument.Occurrence is shown in
Following table.
According to testing result, using T/C value as X-axis, regression analysis is carried out by Y-axis of concentration value, obtains regression equation y=
0.5843e10.793x, goodness of fit R2=0.98, acquired results and regression curve comparison diagram are as shown in Figure 3.
It can be observed by the above results, the kit in the present invention has high sensitivity, stability good and "dead"
The features such as pollution.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. set in a kind of IGF-1 fluorescence immune chromatography detection kit, including detection casing clamping body (1) and detection casing clamping body (1)
Test strips (2), it is characterised in that；The main body of the test strips (2) is bonded by backing bottom plate (4), backing bottom plate (4) upper end
Nitrocellulose filter (5), the sample bonding pad (6) that is bonded of nitrocellulose filter (5) left and right ends and absorbing membrane (7) structure
At；
Nitrocellulose filter (5) upper end has been respectively arranged on the left side and the right side detection line (501) and nature controlling line (502)；
The bonding pad that the sample bonding pad (6) is bonded by the sample pad (601) and sample pad (601) side-lower of layer structure
(602) it constitutes.
2. a kind of IGF-1 fluorescence immune chromatography detection kit according to claim 1, it is characterised in that: the combination
The first monoclonal antibody of time-resolved fluorescence mass signatures is coated on pad (602), and bonding pad (602) is by glass fibre element
Film is dry after surfactant buffer immersion treatment and obtains, and the time-resolved fluorescence substance is lanthanide series substance.
3. a kind of IGF-1 fluorescence immune chromatography detection kit according to claim 1, it is characterised in that: the detection
It is coated with another monoclonal antibody on line (501), is coated with secondary antibody on the nature controlling line (502).
4. a kind of IGF-1 fluorescence immune chromatography detection kit according to claim 2, it is characterised in that: the detection
The upper end of casing clamping body (1) is equipped with the test paper slot (101) for mutually blocking the trough body structure matched with test strips (2), the test paper slot (101)
Side is equipped with the hinged groove (102) of trough body structure, and the fixed hinge for being inserted with cylindrical structure in the groove body of hinged groove (102)
Spindle (103) is hinged with the gland (3) of plate structure in the outer circle of the articulated shaft (103).
5. a kind of IGF-1 fluorescence immune chromatography detection kit according to claim 2, it is characterised in that: the gland
(3) lower end and the upper surface of test strips (2) mutually press, and the middle position of gland (3) upper surface is equipped with frame structure
Well (301).
6. a kind of IGF-1 fluorescence immune chromatography detection kit according to claim 2, it is characterised in that: the sample
Pad (601) is that glass fibre element film is dried after surfactant buffer immersion treatment and obtained.
7. a kind of system of the IGF-1 time-resolved fluoroimmunoassay chromatography detection kit as described in claim 1-6 any one
Preparation Method, it is characterised in that: the preparation method the following steps are included:
S1: prepared by detection line (T line) and nature controlling line (C line), first affix to nitrocellulose filter on backing bottom plate, uses
The anti-human IGF-1 monoclonal antibody of mouse is diluted to 0.5mg/mL by 15mmol/L pH7.4 phosphate buffer, is used to prepare T line；
Sheep anti-mouse antibody is diluted to 1mg/mL with 15mmol/L pH7.4 phosphate buffer, is used to prepare C line；Liquid is drawn by 1uL/cm
Amount is uniformly drawn the antibody after above two dilution to preparation T line and C line on nitrocellulose filter with film instrument is drawn；It will pull
Nitrocellulose filter be placed in 37 DEG C of drying boxes dry 3-4h；
S2: the preparation of bonding pad is resisted the rabbit-anti people IGF-1 for being marked with time-resolved fluorescence substance latex beads by drawing film instrument
Body is sprayed on the polyester film that width is 6-9mm according to the flow of 6uL/cm, and bonding pad is prepared in 37 DEG C of dry 3-4h
S3: sample pad processing, after sample pad carries out closing in advance with the buffer immersion containing surfactant, 50 DEG C dried
Night, herein buffer are as follows: 100mM pH8.0Tris.Hcl buffer, wherein containing 2%BSA, 0.5%PEG 4000,0.1%
Tween-20 and 5% sucrose；
S4: sample pad obtained in abovementioned steps, fixed laminate of bonding pad are pasted onto nitrocellulose filter by test strips assembling
Nearly T line end, and water absorption pad is fixed and laminates the other end for being pasted onto nitrocellulose filter；
S5: the assembling of test strips finished product and detection, progress test strips finished product assembling first, by the good big paperboard item cutting machine of column
It is cut by the width of every 2.5-4mm, the test strips clamping after then cutting out is into test casing clamping body；Test strips inspection
It surveys, assembled test strips is detected with double-antibody method: sample to be tested about 100uL being taken to be added drop-wise in sample pad, it is to be measured
Sample is blood sample and dilution liquid mixture, and the IGF-1 in sample is formed anti-in conjunction with the monoclonal antibody marked on bonding pad
Original-labelled antibody compound continues chromatography to C when nitride layer to be composite is analysed to T line in conjunction with another immobilised antibody herein
Compound is formed with goat anti-rabbit antibody at line, under the excitation of Time-resolved fluorescence assay instrument, labelled element shows glimmering
Light, the labelled antibody that T knot is closed is more, and fluorescence intensity is higher, and T line fluorescence intensity is directly proportional to the IGF-1 level in sample.
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| CN110609135A (en) * | 2019-10-22 | 2019-12-24 | 中秀科技股份有限公司 | Kit for double-antibody sandwich fluorescence chromatography and preparation method thereof |
| CN111220806A (en) * | 2020-03-09 | 2020-06-02 | 上海雄图生物科技有限公司 | Method for manufacturing ultrasensitive rapid time-resolved fluorescence immunochromatographic test strip |
| CN111879938A (en) * | 2020-06-16 | 2020-11-03 | 烟台市疾病预防控制中心 | Quantum dot immunochromatography test strip for rapidly detecting pyrimethanil residues and preparation method |
| CN114280312A (en) * | 2020-09-27 | 2022-04-05 | 河北特温特生物科技发展有限公司 | Whole blood separation membrane for immunofluorescence chromatography detection and preparation method and application thereof |
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