CN211014311U

CN211014311U - Immunochromatographic test paper card of fipronil colloidal gold method

Info

Publication number: CN211014311U
Application number: CN201921841506.XU
Authority: CN
Inventors: 盛相国
Original assignee: Suzhou Kuaijiekang Biotechnology Co ltd
Current assignee: Suzhou Kuaijiekang Biotechnology Co ltd
Priority date: 2019-10-30
Filing date: 2019-10-30
Publication date: 2020-07-14
Anticipated expiration: 2029-10-30

Abstract

The utility model relates to a fipronil colloid gold method immunochromatography test paper card, be in including box body and setting the test paper strip of fipronil detection usefulness in the box body, still be provided with dry chamber in the box body, the dry intracavity is provided with the drier, be provided with observation window and the application of sample hole that are located the coplanar on the box body, its characterized in that: the box body comprises a base and a face cover which are embedded, sealing glue is adhered to the interface of the base and the face cover, a colorless transparent magnifier parallel to the face cover is embedded in the observation window, sealing glue is arranged at the joint of the colorless transparent magnifier and the observation window, and sealing films which can be torn are covered on the sample adding hole and the observation window; the utility model has the advantages that: effectively improve the leakproofness of test paper card to effectively reduce the probability that unused test paper card received external pollution inefficacy, and the observation window sets up the colorless transparent magnifying glass that is on a parallel with the face lid, enlargies the observation to the detection line, observes more convenient accuracy.

Description

Immunochromatographic test paper card of fipronil colloidal gold method

Technical Field

The utility model relates to a pesticide detection area, concretely relates to fipronil colloid gold method immunochromatography test paper card.

Background

Fipronil (fipronil), also known as fipronil, is a widely used phenylpyrazole insecticide. It interferes the central nervous system of insect body by inhibiting the chloride ion channel regulated by gamma-aminobutyric acid in the central nervous system of insect, thus leading the nerve and muscle of insect to be excited and over excited to die, and has excellent control effect on locust and rice pests such as snout moth's larva, brown planthopper, small brown planthopper and weevil.

However, the negative effects of fipronil are gradually shown, and fipronil can be difficult to metabolize in important organs such as high-fat-containing trachea, particularly adrenal gland and liver, thyroid gland, kidney and the like of mammals, so that the mammals, particularly human beings, vomit and anxiety, and epilepsy can be seriously caused, and the health of the human bodies is damaged.

The application range of fipronil is limited by food and agriculture organization and world health organization of the united nations at present, the method for detecting fipronil residue at home and abroad at present mainly adopts chromatography, but the instrument required by the chromatography is high in price, and a complex pretreatment and purification process is required during detection, so that the method is tedious and time-consuming, has higher professional requirement on the operation of technical personnel, is poor in timeliness and is high in cost. The fipronil immunoassay kit has high sensitivity and relatively simple analysis process, but also needs 2-3 hours of detection process and needs an enzyme-labeling instrument for assistance, and the field real-time monitoring cannot be carried out. Therefore, the main means of rapid drug residue screening is currently used in test strip detection, but the test strip is a disposable consumable product, is usually stored in a large quantity, is easily polluted and deteriorated, and is not easily distinguished when the colors of the detection line and the quality control line are observed through the shell during observation.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the purpose is: provides a fipronil colloidal gold immunochromatographic test paper card.

The technical scheme of the utility model is that: the utility model provides a fipronil colloid gold method immunochromatography test paper card, is in including box body and setting the test paper strip that the box body fipronil detected the usefulness, still be provided with dry chamber in the box body, the dry intracavity is provided with the drier, be provided with observation window and the application of sample hole that is located on the same face on the box body, the box body is including the base and the face lid of looks gomphosis, the base is stained with sealed glue with the looks interface of face lid, the observation window embedded be equipped with the parallel colorless transparent magnifying glass of face lid, colorless transparent magnifying glass with the junction of observation window is provided with sealing glue, it has the envelope that can tear to cover on application of sample hole and the observation window.

Further: the test strip comprises a bottom plate, and a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad which are arranged on the bottom plate and are tightly connected in sequence; the sample adding hole corresponds to the position of the sample pad on the test strip, the observation window corresponds to the position of the nitrocellulose membrane on the test strip, the nitrocellulose membrane is provided with a quality control line and a detection line for detecting fipronil, and the detection line is positioned on one side close to the sample pad.

Further: the quality control line is a goat anti-mouse IgG antibody printed line or a rabbit anti-mouse IgG antibody printed line or a goat anti-rabbit IgG antibody printed line or an SPA printed line, the detection line is a carrier protein printed line coupled with fipronil, and the quality control line and the detection line are parallel to each other.

Further: and the bottom plate is also provided with protective films, and the protective films are positioned at two ends of the bottom plate and are respectively connected with the outer edge of the sample pad and the outer edge of the water absorption pad.

Further: the bottom plate is a hard plastic strip or a hard paper strip which does not absorb water.

Further: the observation window all sets up with the application of sample hole the face is covered, the base internal partitioning have the test paper chamber that is linked together with dry chamber, the test paper strip sets up the test paper intracavity.

Compared with the prior art, the utility model has the advantages that: effectively improve the leakproofness of test paper card to effectively reduce the probability that unused test paper card received external pollution inefficacy, and the observation window sets up the colorless transparent magnifying glass that is on a parallel with the face lid, enlargies the observation to the detection line, observes more convenient accuracy.

Drawings

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by the ordinary skilled person in the art without creative work all belong to the protection scope of the present invention:

fig. 1 is a perspective view of the present invention;

FIG. 2 is a schematic view of the structure of the middle box body of the present invention;

FIG. 3 is a schematic structural view of a pilot test paper strip of the present invention;

wherein: 1. a box body; 1a, a base; 1b, a face cover; 2. a drying chamber; 3. an observation window; 4. a sample application hole; 5. a colorless transparent magnifier; 6. sealing the film; 7. a test paper cavity; 8. a base plate; 9. a sample pad; 10. a gold label pad; 11. a nitrocellulose membrane; 12. a water absorbent pad; 13. a quality control line; 14. and detecting lines.

Detailed Description

Example 1: combine figure 1 and the colloidal gold immunochromatography test paper card of fig. 2 show of fipronil, be in including box body 1 and setting the test paper strip 2 of fipronil detection usefulness in the box body 1, still be provided with dry chamber 2 in the box body 1, be provided with the drier in the dry chamber 2, be provided with observation window 3 and the sample application hole 4 that are located the coplanar on the box body 1, box body 1 is including the base 1a and the face lid 1b of looks gomphosis, observation window 3 all sets up with sample application hole 4 on the face lid 1b, the inside partition of base 1a have the test paper chamber 7 that is linked together with dry chamber 2, the test paper strip sets up in the test paper chamber 7, in this embodiment, dry chamber 2 has two and is located the both ends of test paper chamber 7 respectively, and drying effect is better.

The sealing device is characterized in that a sealing adhesive is adhered to the joint surface of the base 1a and the face cover 1b, the base 1a and the face cover 1b are sealed, external pollutants are effectively prevented from entering the box body 1 from the joint gap of the base 1a and the face cover 1b, a colorless transparent magnifier 5 parallel to the face cover 1b is embedded in the observation window 3, sealing glue is arranged at the joint of the colorless transparent magnifier 5 and the observation window 3, the colorless transparent magnifier 5 is effectively prevented from slipping and shifting, the pollutants are prevented from entering the box body 1 from the joint gap, the sample adding hole 4 and the observation hole 3 are covered with a sealing film 6 which can be torn, the sealing film 6 completely covers the sample adding hole 4 and the observation window 3 respectively, the edge of the sealing film 6 is adhered to the face cover 1b through the adhesive sticker, no adhesive sticker is arranged on the contact surface of the sample adding hole 4 and the observation window 3, and the box body 1 is sealed when the box body is not opened, the external pollution is avoided, and the colorless transparent magnifier 5 in the observation window 3 is prevented from being scratched and abraded by external hard objects to influence the observation.

The test strip comprises a bottom plate 8, and a sample pad 9, a gold label pad 10, a nitrocellulose membrane 11 and a water absorption pad 12 which are arranged on the bottom plate 8 and are tightly connected in sequence; fipronil monoclonal antibody-colloidal gold marker in the gold mark pad 10, have the application hole 4 with sample pad 9 position on the test paper strip is corresponding, the nitrocellulose membrane 11 position on observation window 3 and the test paper strip is corresponding, be equipped with quality control line 13 and the detection line 14 that fipronil detected usefulness on the nitrocellulose membrane 11, detection line 14 is located the one side that is close to sample pad 9. In this embodiment, the quality control line 13 is a goat anti-mouse IgG antibody printing line, the detection line 14 is a carrier protein printing line coupled to fipronil, and the quality control line 13 and the detection line 14 are parallel to each other.

And the bottom plate 8 is also provided with protective films, and the protective films are positioned at two ends of the bottom plate 8 and are respectively connected with the outer edge of the sample pad 9 and the outer edge of the water absorption pad 12. In this embodiment, the protective film is a transparent adhesive film, and the bottom plate 8 is a rigid plastic strip.

During detection, the test paper card is horizontally placed, the sealing glue 6 is torn, the sample liquid to be detected is dripped into the sample adding hole 4, and the color is observed from the observation window 3. After being combined with the fipronil monoclonal antibody-colloidal gold marker (gold-labeled antibody) in the gold-labeled pad 10, the sample solution to be detected is diffused to the nitrocellulose membrane 11 together; if the content of the fipronil in the sample liquid to be detected is high, the fipronil in the sample liquid to be detected can be combined with the gold-labeled antibody in the diffusion process, so that the antigen binding site of the fipronil on the gold-labeled antibody is completely sealed, the gold-labeled antibody is prevented from being combined with the carrier protein coupled with the fipronil on the nitrocellulose membrane 11, the detection line 14 does not develop color, the goat anti-mouse IgG (quality control line 13) can be combined with the gold-labeled antibody, and the quality control line 13 develops color; if the content of the fipronil in the sample liquid to be detected is low or zero, the antigen binding site on the gold-labeled antibody cannot be closed, the gold-labeled antibody is combined with the carrier protein coupled with the fipronil on the nitrocellulose membrane 11, the detection line 14 is colored, and meanwhile, the quality control line 13 can also be combined with the gold-labeled antibody, and the quality control line 13 is colored. If the quality control line 13 does not develop color, the test strip is disabled.

Example 2: in this example, the control line 13 is a straight line printed with rabbit anti-mouse IgG antibody, and the bottom plate is a non-absorbent hard paper strip, which is otherwise the same as example 1.

Example 3: in this example, the quality control line 13 is a straight line printed with goat anti-rabbit IgG antibody, and the bottom plate is a non-absorbent hard paper strip, which is otherwise the same as example 1.

Example 4: in this embodiment, the quality control lines 13 are SPA printing straight lines, and the others are the same as those in embodiment 1.

It is to be noted that, in this document, terms such as "comprises" or any other variation thereof are intended to cover a non-exclusive inclusion, such that an article or apparatus including a series of elements includes not only those elements but also other elements not explicitly listed or inherent to such article or apparatus.

The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose of the embodiments is to enable those skilled in the art to understand the contents of the present invention and to implement the present invention, which cannot limit the protection scope of the present invention. It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art will be able to make the description as a whole, and the embodiments may be appropriately combined to form other embodiments as will be apparent to those skilled in the art.

Claims

1. The utility model provides a fipronil colloid gold method immunochromatography test paper card, is in including box body and setting the test paper strip of fipronil detection usefulness in the box body, still be provided with dry chamber in the box body, the dry intracavity is provided with the drier, be provided with observation window and the application of sample hole that are located the coplanar on the box body, its characterized in that: the box body is including the base and the face lid of looks gomphosis, the face of meeting of base and face lid is stained with sealed glue, the observation window is embedded be equipped with the parallel colorless transparent magnifying glass of face lid, colorless transparent magnifying glass with the junction of observation window is provided with sealing glue, it has the envelope that can tear to all cover on application of sample hole and the observation window.

2. The fipronil colloidal gold immunochromatographic test paper card according to claim 1, which is characterized in that: the test strip comprises a bottom plate, and a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad which are arranged on the bottom plate and are tightly connected in sequence; the sample adding hole corresponds to the position of the sample pad on the test strip, the observation window corresponds to the position of the nitrocellulose membrane on the test strip, the nitrocellulose membrane is provided with a quality control line and a detection line for detecting fipronil, and the detection line is positioned on one side close to the sample pad.

3. The fipronil colloidal gold immunochromatographic test paper card according to claim 2, which is characterized in that: the quality control line is a goat anti-mouse I gG antibody printed line or a rabbit anti-mouse I gG antibody printed line or a goat anti-rabbit I gG antibody printed line or an SPA printed line, the detection line is a carrier protein printed line coupled with fipronil, and the quality control line and the detection line are parallel to each other.

4. The fipronil colloidal gold immunochromatographic test paper card according to claim 2, which is characterized in that: and the bottom plate is also provided with protective films, and the protective films are positioned at two ends of the bottom plate and are respectively connected with the outer edge of the sample pad and the outer edge of the water absorption pad.

5. The fipronil colloidal gold immunochromatographic test paper card according to claim 4, which is characterized in that: the bottom plate is a hard plastic strip or a hard paper strip which does not absorb water.

6. The fipronil colloidal gold immunochromatographic test paper card according to claim 1, which is characterized in that: the observation window all sets up with the application of sample hole the face is covered, the base internal partitioning have the test paper chamber that is linked together with dry chamber, the test paper strip sets up the test paper intracavity.