CN219456182U

CN219456182U - Myocardial damage detection card based on colloidal gold method

Info

Publication number: CN219456182U
Application number: CN202320364857.6U
Authority: CN
Inventors: 裘科斌; 张晖; 徐文淑
Original assignee: Safecare Boitech Hangzhou Co ltd
Current assignee: Safecare Boitech Hangzhou Co ltd
Priority date: 2023-02-24
Filing date: 2023-02-24
Publication date: 2023-08-01
Anticipated expiration: 2033-02-24

Abstract

The utility model discloses a myocardial damage detection card based on a colloidal gold method, which aims to solve the problems of complex structure and complicated detection in the prior art.

Description

Myocardial damage detection card based on colloidal gold method

Technical Field

The utility model belongs to a disease detection technology, in particular to a myocardial damage detection card based on a colloidal gold method.

Background

The immunological detection technology is an experimental means for determining antigens, antibodies, immune cells, chemical components and the like by applying the design of the immunological principle, and is widely used for samples which are derived from human bodies and animal bodies and can be used for disease diagnosis and health detection and for environmental, pharmaceutical analysis, food and industrial analysis. The commonly used immunological detection techniques include immunological turbidity techniques, solid phase enzyme immunoassay techniques, chemiluminescent detection techniques, immunofluorescence labeling techniques, flow cytometry, colloidal gold techniques, etc.

The colloidal gold technology is a common detection technology and is widely applied to detection of myocardial injury markers including, but not limited to, cTnI, creatine Kinase-MB, myoglobin, NT-proBNP and D-Dimer, so as to realize clinical diagnosis of cardiovascular diseases such as myocardial infarction, membrane injury and the like.

However, the prior art is not perfect, in the detection process, a test tube filled with whole blood is required to be centrifuged, then serum is extracted by adopting a dropper, and a sample can be dripped on a detection card through the dropper extracted with the serum to complete clinical detection.

Disclosure of Invention

In order to overcome the defects and the existing problems in the prior art, the utility model provides a myocardial damage detection card based on a colloidal gold method.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a myocardial damage detection card based on colloidal gold method, includes the hose and inserts the detection box of establishing in the hose, is provided with the test paper that is used for detecting myocardial damage marker in the detection box, and the detection box includes the sampling hole, and the sampling hole upper cover is established at the sealing membrane, and sampling hole and sealing membrane set up in the hose, are formed with the drainage groove in the hose, are formed with the blade in the drainage groove, and the blade is used for cutting the sealing membrane.

Preferably, the hose comprises an elastic thin wall protruding outwards, the elastic thin wall is surrounded into a drainage groove, and the blade is formed in the elastic thin wall.

Preferably, the bottom of the hose comprises a V-shaped plate, and the cross section of the V-shaped plate is V-shaped.

Preferably, a countersunk groove is formed at the outer end of the sampling hole, and the sealing film is fixedly arranged in the countersunk groove.

Preferably, the detection box comprises an upper cover and a lower cover, wherein the upper cover is detachably arranged on the lower cover, and the detection test paper is arranged between the upper cover and the lower cover.

Preferably, the upper cover comprises a plug, the lower cover comprises a slot, and the plug is in interference fit with the slot.

Preferably, the lower cover is provided with a positioning groove, the detection test paper is arranged in the positioning groove, the upper cover is provided with a pressing plate, and the pressing plate is used for limiting the detection test paper in the positioning groove.

Preferably, the number of the pressing plates is at least three, and more than three pressing plates are sequentially arranged in the length direction of the test paper.

Preferably, the detection test paper comprises a sampling pad, a filtering pad, a combining pad, a nitrocellulose membrane and water absorbing filter paper which are sequentially arranged together, wherein a quality control section and a detection section are arranged on the nitrocellulose membrane, and the combining pad, the detection section, the quality control section and the water absorbing filter paper are sequentially arranged on the nitrocellulose membrane, and the sampling pad is opposite to the sampling hole.

Compared with the prior art, the utility model has the following outstanding and beneficial technical effects:

in the utility model, after a sample is filled in the hose, the myocardial damage detection card based on the colloidal gold method can be placed on a centrifugal machine for centrifugal treatment, then the blade on the hose is extruded to cut the sealing film, and then the hose is extruded to enable the sample to be added on the detection test paper along the drainage groove and the sampling hole without using a dropper, so that the myocardial damage detection card based on the colloidal gold method has the advantages of simple structure, simplicity and convenience in operation and high detection efficiency.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a schematic structural view of the cartridge of the present utility model;

FIG. 3 is a schematic view of the cartridge of the present utility model after removal of the sealing membrane;

FIG. 4 is a schematic cross-sectional view of the cartridge of the present utility model;

FIG. 5 is a schematic cross-sectional view of the test strip of the present utility model;

FIG. 6 is a schematic cross-sectional view of the lower cover of the present utility model;

FIG. 7 is a schematic cross-sectional view of the upper cover of the present utility model;

FIG. 8 is a schematic cross-sectional view of a hose of the present utility model;

FIG. 9 is a second schematic cross-sectional view of the hose of the present utility model;

in the figure: 1-hose, 2-detection box, 3-test paper, 4-blade, 11-elastic thin wall, 12-drainage groove, 13-V template, 21-upper cover, 22-lower cover, 23-sealing film, 211-sampling hole, 212-countersink, 213-plug, 214-pressing plate, 215-observation window, 221-slot, 222-positioning groove, 31-sampling pad, 32-filter pad, 33-combination pad, 34-nitrocellulose film, 35-water absorbing filter paper, 341-quality control section and 342-detection section.

Detailed Description

The present utility model is further described below with reference to the drawings and specific embodiments for the purpose of facilitating understanding by those skilled in the art.

As shown in fig. 1 to 9, a myocardial damage test card based on a colloidal gold method includes a flexible tube 1 and a test cartridge 2.

The hose 1 is made of a soft and elastic material. The soft and resilient material may be polypropylene or high density polyethylene. The whole structure of the hose 1 is a test tube with a tube orifice at the upper end. In actual use, the hose 1 can be filled with a sample, and the sample in the hose 1 can be extruded to the tube mouth of the test tube by extruding the hose 1.

The detection box 2 is used for detecting myocardial damage markers on a sample. The detection box 2 is internally provided with detection test paper 3, and the detection test paper 3 is used for detecting myocardial damage markers. The test cassette 2 includes a sampling hole 211, the sampling hole 211 faces the test paper 3 in the test cassette 2, and in actual use, a sample can be added to the test paper in the test cassette 2 through the sampling hole 211.

The hose 1 is inserted into the detection box 2. The detection box 2 plays a role in plugging the pipe orifice of the hose 1, and is equivalent to a 'plug' of the hose 1. The upper cover 21 of the sampling hole 211 is provided with a sealing film 23, and the sealing film 23 plays a role in blocking the sampling hole 211, so that the sample is prevented from entering the detection box 2 during centrifugation. A drainage groove 12 is formed in the hose 1, the drainage groove 12 is arranged on the sealing film 23, in actual use, the hose 1 is extruded, and a sample in the hose 1 can reach the sealing film 23 under the drainage of the drainage groove 12. The blade 4 is formed in the drainage groove 12, the blade 4 is used for cutting the sealing film 23, the blade 4 can be made of hard plastic and welded in the drainage groove 12, in actual use, the blade 4 on the hose 1 is extruded, the blade 4 can cut the sealing film 23, the sealing film 23 can be cut, and a sample drained through the drainage groove 12 can enter the sampling hole 211 through the cut in the sealing film 23.

In the utility model, after a sample is filled in the hose 1, the myocardial damage detection card based on the colloidal gold method can be placed on a centrifuge for centrifugal treatment, then the sealing film 23 is cut by the blade 4 on the hose 1, and then the hose 1 is extruded so that the sample is added on the detection test paper 3 along the drainage groove 12 and the sampling hole 211 without using a dropper, therefore, the myocardial damage detection card based on the colloidal gold method has the advantages of simple structure, simple and convenient operation and high detection efficiency.

The hose 1 comprises an elastic thin wall 11 protruding outwards, the elastic thin wall 11 is surrounded to form a drainage groove 12, the blade 4 is formed in the elastic thin wall 11, in actual use, a user can squeeze the elastic thin wall 11 to drive the blade 4 to move towards the sealing film 23, and the design of the elastic thin wall 11 is convenient for the user to press the blade 4.

The bottom of the hose 1 comprises a V-shaped plate 13, and the cross section of the V-shaped plate 13 is V-shaped. In actual use, the V-shaped plates 13 can be folded together when the user squeezes the hose 1, so that the sample in the hose 1 can be quickly moved into the drainage groove 12.

The outer end of the sampling hole 211 is provided with a countersunk groove 212, the sealing film 23 is fixedly arranged in the countersunk groove 212, the sealing film 23 can be adhered in the countersunk groove 212, and the design of the countersunk groove 212 improves the surface flatness of the detection box 2.

The detection box 2 comprises an upper cover 21 and a lower cover 22, the upper cover 21 is detachably arranged on the lower cover 22, the detection test paper 3 is arranged between the upper cover 21 and the lower cover 22, and the upper cover 21 and the lower cover 22 are designed so that the detection test paper 3 is arranged in the detection box 2.

The upper cover 21 includes a plug 213, and the lower cover 22 includes a slot 221, and the plug 213 is interference fit in the slot 221, so as to realize detachable connection of the upper cover 21 and the lower cover 22.

The lower cover 22 is provided with a positioning groove 222, the test paper 3 is arranged in the positioning groove 222, the upper cover 21 is provided with a pressing plate 214, and the pressing plate 214 is used for limiting the test paper 3 in the positioning groove 222, so that the connection firmness is improved.

The number of the pressing plates 214 is three, and the three pressing plates 214 are uniformly arranged in the length direction of the test paper 3.

The test paper 3 comprises a sampling pad 31, a filter pad 32, a combination pad 33, a nitrocellulose membrane 34 and a water absorbing filter paper 35 which are sequentially arranged together, a quality control section 341 and a detection section 342 are arranged on the nitrocellulose membrane 34, and the combination pad 33, the detection section 342, the quality control section 341 and the water absorbing filter paper 35 are sequentially arranged on the nitrocellulose membrane 34, and the sampling pad 31 is opposite to the sampling hole 211.

The length of the sampling pad 31 is 9mm, the sampling pad 31 is used for absorbing a sample dripped on test paper, and the sampling pad 31 is made of a glass fiber membrane. In actual use, the sample may flow rapidly to filter pad 32 under capillary action of sampling pad 31. The filter pad 32 has a length of 11mm and is used for filtering impurities in the sample and reducing interference factors in the sample.

The length of the conjugate pad 33 is 5mm, and the myocardial protein type I detection antigen and the quality control substance marked by the marker are coated on the conjugate pad 33. The label refers to a substance with color display, such as fluorescent microsphere, colloidal gold, colored microsphere, etc., which can be combined with the antibody.

The detection section 342 and the quality control section 341 are spaced apart from each other and disposed in parallel on the nitrocellulose membrane 34. The detection segment 342 is composed of a novel coronavirus monoclonal capture antigen recognizing a single antibody epitope, the detection segment 342 is coated on the nitrocellulose membrane 34, and the detection segment 342 is adjacent to the binding pad 33. The quality control section 341 is formed by adopting quality control capture antigen, the quality control section 341 is coated on the nitrocellulose membrane 34, and the quality control section 341 is close to the water absorption filter paper 35. The length of the water absorbing filter paper 35 was 6mm.

The upper cover 21 is provided with an observation window 215, and in actual use, a user can observe the change of the test paper 3 through the observation window 215.

The above embodiments are only preferred embodiments of the present utility model, and are not intended to limit the scope of the present utility model in this way, therefore: all equivalent changes in structure, shape and principle of the utility model should be covered in the scope of protection of the utility model.

Claims

1. The utility model provides a myocardial damage detects card based on colloidal gold method, a serial communication port, including hose (1) and insert detection box (2) of establishing in hose (1), be provided with in detection box (2) and be used for detecting myocardial damage marker's test paper (3), detection box (2) are including sampling hole (211), sampling hole (211) upper cover (21) are established at sealing membrane (23), sampling hole (211) and sealing membrane (23) set up in hose (1), be formed with drainage groove (12) in hose (1), be formed with blade (4) in drainage groove (12), blade (4) are used for cutting sealing membrane (23).

2. The card for detecting myocardial damage based on the colloidal gold method according to claim 1, wherein the hose (1) comprises an elastic thin wall (11) protruding outwards, the elastic thin wall (11) is surrounded into a drainage groove (12), and the blade (4) is formed in the elastic thin wall (11).

3. The myocardial damage test card based on the colloidal gold method as recited in claim 1, wherein the bottom of the hose (1) comprises a V-shaped plate (13), and the cross section of the V-shaped plate (13) is V-shaped.

4. The card for detecting myocardial damage based on the colloidal gold method as recited in claim 1, wherein the outer end of the sampling hole (211) is formed with a countersunk groove (212), and the sealing film (23) is fixedly arranged in the countersunk groove (212).

5. The card for detecting myocardial damage based on the colloidal gold method according to claim 1, wherein the detection box (2) comprises an upper cover (21) and a lower cover (22), the upper cover (21) is detachably arranged on the lower cover (22), and the detection test paper (3) is arranged between the upper cover (21) and the lower cover (22).

6. The card for detecting myocardial damage based on the colloidal gold method according to claim 5, wherein the upper cover (21) comprises a plug (213), the lower cover (22) comprises a slot (221), and the plug (213) is interference fit in the slot (221).

7. The card for detecting myocardial damage based on the colloidal gold method according to claim 5 or 6, wherein a positioning groove (222) is formed on the lower cover (22), the test paper (3) is disposed in the positioning groove (222), a pressing plate (214) is formed on the upper cover (21), and the pressing plate (214) is used for limiting the test paper (3) in the positioning groove (222).

8. The card for detecting myocardial damage based on the colloidal gold method according to claim 7, wherein at least three pressing plates (214) are provided, and more than three pressing plates (214) are sequentially provided in the length direction of the test paper (3).

9. The myocardial damage detection card based on the colloidal gold method according to claim 1, wherein the detection test paper (3) comprises a sampling pad (31), a filtering pad (32), a combining pad (33), a nitrocellulose membrane (34) and a water absorbing filter paper (35) which are sequentially arranged together, a quality control section (341) and a detection section (342) are arranged on the nitrocellulose membrane (34), and the combining pad (33), the detection section (342) and the quality control section (341) and the water absorbing filter paper (35) are sequentially arranged on the nitrocellulose membrane (34), and the sampling pad (31) is opposite to the sampling hole (211).