CN211356041U - Head fixing device and two-photon imaging auxiliary system - Google Patents
Head fixing device and two-photon imaging auxiliary system Download PDFInfo
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- CN211356041U CN211356041U CN201921131016.0U CN201921131016U CN211356041U CN 211356041 U CN211356041 U CN 211356041U CN 201921131016 U CN201921131016 U CN 201921131016U CN 211356041 U CN211356041 U CN 211356041U
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Abstract
The utility model belongs to biomedical formation of image field discloses a head fixing device, include: a stage; the head frame is used for fixing the head of the experimental animal; two head frame fixing parts arranged on the carrying platform and respectively connected with one end of the head frame; two first height adjustment members, each first height adjustment member drivingly connected to one of the head mount members, configured to individually adjust the height of each head mount member relative to the stage. The utility model also discloses a two-photon imaging auxiliary system who has above-mentioned head fixing device. The utility model discloses an above-mentioned head fixing device, by the fixed headstock of two headstock mountings, realize the independent control of two headstock mounting heights through first altitude mixture control spare afterwards, and then can the accurate focusing imaging plane to level of adjusting, improve experimental quality.
Description
Technical Field
The utility model relates to a biomedical formation of image field especially relates to a head fixing device and two-photon formation of image auxiliary system.
Background
The brain is the most complex organ of the human body and the study of its structure and function has been a great challenge. The brain science has become the leading research field of the 21 st century, and the research result of the brain science not only is beneficial to the prevention and treatment of brain diseases, but also can generate great promotion effect on the development of information and intelligent industries. At present, a two-photon microscope adopts near-infrared ultrafast laser as an excitation light source, and is particularly suitable for real-time imaging of living cranial nerve cells, glial cells and the like because the two-photon microscope has low cytotoxicity and deeper penetration on samples. Two-photon imaging has become an important indispensable experimental means in brain science research to date.
In the two-photon living body brain imaging process, the brain of a sample needs to be fixed firstly, so that the stability of an animal brain cell observation plane image is ensured, and meanwhile, the stability of the vital signs of a living body animal is also ensured, so that a long-time high-quality fine imaging image is obtained. However, most of the commonly used two-photon microscope lens holder fixing devices are simple fixing frames, and are difficult to accurately adjust a focusing imaging plane of mouse brain tissue, and after a commercialized mouse adapter is used, the mouse adapter is easy to move in an observation process to cause imaging image displacement, and meanwhile, the special fixing mode of the mouse adapter can cause increase of mouse cranial pressure, so that the state of glial cells is abnormal. Secondly, the commonly used two-photon microscope lens frame fixing device is mostly used for animals after abdominal cavity injection anesthesia, the anesthesia depth and the anesthesia time are difficult to control, the animals are easy to be awake when the anesthesia is shallow, and the respiration and heartbeat under the shallow anesthesia state can influence the observation record, so that the two-photon microscope lens frame fixing device is not suitable for long-time stable image acquisition. Meanwhile, in order to maintain the normal operation of the instrument, the temperature control of the two-photon microscope laboratory is generally 20 ℃, the temperature is easy to cause hypothermia or accidental death of the anesthetized mouse, the existing two-photon microscope lens frame fixing device is not compatible with a body temperature detection and control device, and in addition, an externally-matched body temperature detection pad is also easy to move in the imaging process and is not beneficial to maintaining the physical signs of the mouse in the long-time imaging process.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a head fixing device and two-photon formation of image auxiliary system can focus imaging plane to level by the accurate adjustment, improves experiment quality.
To achieve the purpose, the utility model adopts the following technical proposal:
a head fixation device comprising:
the carrying platform is used for placing the experimental animal;
the head frame is used for fixing the head of the experimental animal;
the two head frame fixing parts are arranged on the carrying platform and are respectively connected with one end of the head frame;
two first height adjustment members, each of the first height adjustment members drivingly connected to one of the head mount members, configured to individually adjust the height of each of the head mount members relative to the stage.
Preferably, each first height adjusting part comprises a frame body, a rotatable screw rod is arranged on the frame body in a penetrating mode, a sliding block is connected to the screw rod in a threaded mode, the sliding block is slidable in the vertical direction along the frame body, a knob is fixedly connected to one end of the screw rod, and the headstock fixing part is installed on the sliding block.
Preferably, each first height adjusting member includes a frame body and a linear driving member disposed on the frame body, and an output end of the linear driving member is connected to the head frame fixing member and is configured to drive the head frame fixing member to ascend and descend.
Preferably, the anesthesia apparatus further comprises an anesthesia mask fixing part and a second height adjusting part for driving the anesthesia mask fixing part to ascend and descend, and the second height adjusting part is installed on the carrying platform.
Preferably, the adjustment accuracy of the first height adjusting member and the second height adjusting member is 0.01 mm.
Preferably, the carrier is provided with a temperature adjusting assembly for maintaining the body temperature of the experimental animal and/or adjusting the ambient temperature of the experimental animal.
Preferably, the temperature adjusting assembly comprises a temperature pad, a fixing groove is formed in the carrier, and the temperature pad is arranged in the fixing groove.
Preferably, the carrying platform is provided with a fixing elastic sheet positioned at the edge of the fixing groove, and the fixing elastic sheet is used for clamping and fixing the temperature pad.
Preferably, the carrier is provided with at least two fixed card slots.
The utility model also provides a two-photon imaging auxiliary system, including foretell head fixing device, still include:
the microscope comprises a two-photon microscope, wherein the carrying platform is fixed on a carrying platform of the two-photon microscope;
the anesthesia device comprises an anesthesia breathing mask, and the anesthesia breathing mask is fixed by an anesthesia mask fixing part;
and the body temperature monitoring device is used for monitoring the body temperature of the experimental animal.
The utility model has the advantages that:
the utility model discloses an above-mentioned head fixing device, by the fixed headstock of two headstock mountings, realize the independent control of two headstock mounting heights through first altitude mixture control spare afterwards, and then can the accurate focusing imaging plane to level of adjusting, improve experimental quality, and can not cause the rising that the experimental animals cranium pressed.
Through setting up the anaesthetic mask mounting, connect anaesthesia device afterwards, can realize gaseous anesthesia and drug anesthesia, can reduce the artifact that breathing and heartbeat brought, reduce experimental animals' mortality.
Through setting up temperature regulation subassembly, can maintain experimental animals's normal physiological function, more accord with experimental animals' ethical experiment standard and animal welfare.
The utility model discloses a two-photon imaging auxiliary system, maintain and the temperature monitoring integration on head fixing device through anesthesia, the temperature with experimental animals, more be fit for long-time experiment and survey.
Drawings
Fig. 1 is a schematic structural view of the head fixation device of the present invention;
FIG. 2 is an enlarged view of FIG. 1 at A;
FIG. 3 is an enlarged view of FIG. 1 at B;
fig. 4 is a schematic structural diagram of the two-photon imaging auxiliary system of the present invention.
In the figure:
1. a stage; 11. fixing grooves; 12. fixing the elastic sheet; 13. fixing the clamping groove; 2. a headstock fixing member; 21. a splint; 3. a first height adjustment member; 31. a frame body; 32. a screw; 33. a slider; 34. a knob; 4. an anaesthetic mask fixing member; 41. a card slot; 5. a second height adjustment member; 6. experimental animals; 10. a head fixing device; 20. a head frame; 30. a two-photon microscope; 40. an anesthesia apparatus; 50. a body temperature monitoring device.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some of the structures related to the present invention are shown in the drawings, not all of the structures.
In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, detachably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
In the description of the present embodiment, the terms "upper", "lower", "right", etc. are used in an orientation or positional relationship based on that shown in the drawings only for convenience of description and simplicity of operation, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used only for descriptive purposes and are not intended to have a special meaning.
The utility model provides a head fixing device 10, it can be applied to two photon imaging auxiliary system for fix and realize the planar horizontal adjustment of focus imaging the head of experimental animals 6, in this embodiment, above-mentioned experimental animals 6 can be the mouse, also can be other small-size animals.
As shown in fig. 1, the head fixing device 10 includes a carrier 1, a head mount holder 2, a first height adjusting member 3, an anesthetic mask holder 4, a second height adjusting member 5, a head mount 20, and a temperature adjusting assembly, wherein:
the stage 1 can be placed on the stage of the two-photon microscope 30 (as shown in fig. 4), and may have a rectangular structure or a plate-like structure having another shape. The platform deck 1 can be used for placing an experimental animal 6 and matching with the two-photon microscope 30 to carry out an experiment. In this embodiment, at least two fixing clip grooves 13 are provided in the stage 1, and the stage 1 can be fixed to the stage of the two-photon microscope 30 by the fixing clip grooves 13. Further, four fixed card slots 13 are provided in the embodiment, and the four fixed card slots 13 are located at four corners of the carrier 1.
The two headstock fixing members 2 are symmetrically arranged on the carrier 1, and are used for fixing two sides of the headstock 20, in this embodiment, the headstock 20 is specifically a structure for fixing the head of the experimental animal 6, and as shown in fig. 4, the headstock can be fixed by a double-sided blade through clamping; other frame structures are also possible, as long as the clamping fixation of the head of the experimental animal 6 can be satisfied. The headgear attachment 2 may be a clamping assembly as shown in fig. 2, i.e., the headgear 20 is clamped and fixed by two clamping plates 21, and then the two clamping plates 21 are fixed by bolts to ensure a stable clamping force. The headgear mount 2 may also be a plate or block structure to which the headgear 20 may be secured by screws.
The first height adjusting member 3 is drivingly connected to the head mount 2, and is capable of driving the head mount 2 to move up and down relative to the carrier 1, so as to adjust the height of the head mount 2. In this embodiment, the first height adjusting component 3 can achieve independent height adjustment of the two headgear fixing components 2, that is, the height of one headgear fixing component 2 can be adjusted as required. Through the height of independent adjustment headstock mounting 2, realize the fine setting to the focusing imaging plane, finally make the focusing imaging plane adjusted to the level, the imaging effect is better, has improved the experiment quality. In addition, the embodiment can also realize the adjustment of the overall height of the focusing imaging plane by simultaneously adjusting the two head frame fixing parts 2 to ascend or descend to the same height.
Optionally, as shown in fig. 2, the first height adjusting part 3 includes two frame bodies 31, a screw rod 32 penetrates through each frame body 31, and the screw rod 32 can rotate around its axis relative to the frame body 31, a sliding block 33 is connected to the screw rod 32 through a thread, a sliding slot (not shown in the figure) is provided in the frame body 31 along the vertical direction, the sliding block 33 is disposed in the sliding slot, and the sliding block 33 can slide in the sliding slot through the rotation of the screw rod 32. A knob 34 is fixedly connected to one end of the screw 32, which penetrates out of the top of the frame 31, and the screw 32 can be rotated by screwing the knob 34. The head mount fixing member 2 is mounted on the slider 33 and slides together with the slider 33. Specifically, as shown in fig. 2, the two clamping plates 21 of the headstock fixing member 2 may be fixed to the slider 33 by bolts, and the bolts may also achieve clamping of the two clamping plates 21. By rotating any one of the knobs 34, the knob 34 can drive the screw 32 connected with the knob to rotate, and the screw 32 drives the corresponding sliding block 33 to vertically move along the sliding groove, so that the sliding block 33 drives the headstock fixing member 2 to lift relative to the frame body 31. And through setting up the spout, can ensure that headstock mounting 2 moves and the stability of removal is higher along with slider 33 along vertical direction, more does benefit to the fine setting of focus imaging plane.
It should be noted that, the first height adjusting element 3 of the present embodiment may further include a frame body 31, and linear driving elements symmetrically disposed on two sides of the frame body 31, wherein an output end of the linear driving element is connected to the headstock fixing element 2, and is used for driving the headstock fixing element 2 to ascend and descend. The linear driving part can be a linear motor or a cylinder and other parts capable of doing linear motion, the headstock fixing part 2 is driven to ascend and descend through the linear driving part, and the automatic adjustment of the height of the headstock fixing part 2 can be achieved. And each linear driving piece drives one headstock fixing piece 2 to lift, so that the height of the headstock fixing piece 2 can be independently adjusted.
In this embodiment, the adjustment precision of the first height adjusting part 3 is 0.01mm, which can realize the precise adjustment of the head mount 2, and further the precise adjustment of the height and the levelness of the focusing imaging plane.
In this embodiment, as shown in fig. 1 and fig. 3, the second height adjusting part 5 is fixed on the carrier 1, the anesthetic mask fixing part 4 is used for fixing the anesthetic breathing mask, and the height of the anesthetic mask fixing part 4 can be adjusted by the second height adjusting part 5, so that the height adjustment of the anesthetic breathing mask is realized, and the anesthetic breathing mask can be covered on the head of the experimental animal 6 to anesthetize the experimental animal 6.
The anesthetic mask fixing member 4 may be, as shown in fig. 3, provided with a clamping groove 41 to clamp and fix the anesthetic breathing mask, or may be of another structure capable of fixing the anesthetic breathing mask. The structure of the second height adjusting member 5 may be the same as that of the first height adjusting member 3, and the adjustment accuracy of the second height adjusting member 5 is 0.01 mm.
The temperature adjusting assembly is arranged on the carrier 1 and used for adjusting the ambient temperature of the experimental animal 6 so as to maintain the normal physiological function of the experimental animal 6 and better accord with the ethical experiment specification and animal welfare of the experimental animal 6.
In this embodiment, the temperature adjustment assembly includes a temperature pad (not shown), a fixing groove 11 is formed on the carrier 1, and the temperature pad is disposed in the fixing groove 11. Meanwhile, the fixed elastic sheet 12 positioned at the edge of the fixed groove 11 is arranged on the carrying platform 1, the fixed elastic sheet 12 is rotatably arranged on the carrying platform 1, and the temperature pad can be prevented from falling off or deviating by rotating the fixed elastic sheet 12 to compress the temperature pad. By the temperature pad, the body temperature of the experimental animal 6 can be made constant, and the temperature around the experimental animal 6 can be always maintained in the optimum experimental temperature range.
It is understood that the temperature adjusting component of the present embodiment may also be a heating wire or a heating tube, and the temperature around the experimental animal 6 is adjusted by electrifying the heating wire or the heating tube.
The head fixing device 10 of the present embodiment is easy to fix the head frame 20 during observation, and can precisely adjust the focusing imaging plane to the horizontal, so as to improve the experimental quality, and compared with the mouse adaptor used in the prior art, the head fixing device 10 of the present embodiment does not cause the increase of the cranial pressure of the experimental animal 6 after long-time observation.
The utility model provides a two-photon imaging auxiliary system, as shown in fig. 4, this two-photon imaging auxiliary system includes foretell head fixing device 10, two-photon microscope 30, anaesthesia device 40 and body temperature monitoring devices 50, wherein: on microscope carrier 1 of headstock mounting 2 was fixed in two-photon microscope 30's objective table, anaesthesia device 40 included anesthesia respirator, and anesthesia respirator is fixed by anesthesia mask mounting 4 for anaesthetizing experimental animals 6, this anesthesia can be gaseous anesthesia and/or drug anesthesia moreover, can reduce the artifact that respiratory and heartbeat brought, reduces experimental animals 6's mortality.
The body temperature monitoring device 50 is used for monitoring the body temperature of the experimental animal 6, and may be a temperature sensor or a temperature probe. By monitoring the body temperature of the laboratory animal 6 and the temperature of its surroundings in real time, it can be ensured that the laboratory animal 6 is in an optimal laboratory temperature environment.
It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Numerous obvious variations, rearrangements and substitutions will now occur to those skilled in the art without departing from the scope of the invention. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. A head fixation device (10) comprising:
a carrier (1) for placing an experimental animal (6);
a head frame (20) for fixing the head of the experimental animal (6);
the two head frame fixing parts (2) are arranged on the carrying platform (1) and are respectively connected with one end of the head frame (20);
two first height adjustment members (3), each first height adjustment member (3) being drivingly connected to one of the head mount (2) and configured to individually adjust the height of each head mount (2) relative to the carrier (1).
2. The head fixation device (10) according to claim 1, wherein each first height adjustment member (3) comprises a frame body (31), a rotatable screw rod (32) is arranged on each frame body (31) in a penetrating manner, a sliding block (33) is connected to each screw rod (32) in a threaded manner, each sliding block (33) is slidable in a vertical direction along the frame body (31), a knob (34) is fixedly connected to one end of each screw rod (32), and the headstock fixation member (2) is mounted on each sliding block (33).
3. The head fixation device (10) according to claim 1, wherein each of the first height adjustment members (3) comprises a frame body (31) and a linear driving member provided on the frame body (31), an output end of the linear driving member being connected to the head mount (2) for driving the head mount (2) to ascend and descend.
4. A head fixation device (10) according to claim 2 or 3, further comprising an anaesthetic mask fixing member (4) and a second height adjustment member (5) for driving the anaesthetic mask fixing member (4) to move up and down, the second height adjustment member (5) being mounted on the carrier (1).
5. A head fixation device (10) according to claim 4, wherein the adjustment accuracy of the first height adjustment member (3) and the second height adjustment member (5) is 0.01 mm.
6. Head fixation device (10) according to claim 1, wherein said carrier (1) is provided with a temperature adjustment assembly for maintaining the body temperature of said laboratory animal (6) and/or adjusting the ambient temperature of said laboratory animal (6).
7. Head fixation device (10) according to claim 6, wherein said temperature regulation means comprise a temperature pad, and wherein said carrier (1) has a fixation groove (11) defined therein, said temperature pad being positioned in said fixation groove (11).
8. The head fixture (10) according to claim 7, wherein the carrier (1) is provided with a fixing spring (12) at the edge of the fixing groove (11), and the fixing spring (12) is used for clamping and fixing the temperature pad.
9. Head fixation device (10) according to claim 1, characterized in that at least two fixation slots (13) are provided on the carrier (1).
10. A two-photon imaging support system comprising the head restraint (10) of any one of claims 1-9, and further comprising:
a two-photon microscope (30), wherein the stage (1) is fixed on the stage of the two-photon microscope (30);
the anesthesia device (40) comprises an anesthesia breathing mask, and the anesthesia breathing mask is fixed by an anesthesia mask fixing part (4);
a body temperature monitoring device (50) for monitoring the body temperature of the laboratory animal (6).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921131016.0U CN211356041U (en) | 2019-07-18 | 2019-07-18 | Head fixing device and two-photon imaging auxiliary system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921131016.0U CN211356041U (en) | 2019-07-18 | 2019-07-18 | Head fixing device and two-photon imaging auxiliary system |
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| CN211356041U true CN211356041U (en) | 2020-08-28 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114209279A (en) * | 2021-04-08 | 2022-03-22 | 浙江大学 | Device for long-time in-vivo imaging of awake small animal |
| JP7141578B1 (en) * | 2021-04-08 | 2022-09-26 | 浙江大学 | Apparatus for long-term intravital imaging of awake small animals |
-
2019
- 2019-07-18 CN CN201921131016.0U patent/CN211356041U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114209279A (en) * | 2021-04-08 | 2022-03-22 | 浙江大学 | Device for long-time in-vivo imaging of awake small animal |
| JP7141578B1 (en) * | 2021-04-08 | 2022-09-26 | 浙江大学 | Apparatus for long-term intravital imaging of awake small animals |
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