CN204092265U - A kind of animal spinal cord living imaging Orientation observation device - Google Patents
A kind of animal spinal cord living imaging Orientation observation device Download PDFInfo
- Publication number
- CN204092265U CN204092265U CN201420617651.0U CN201420617651U CN204092265U CN 204092265 U CN204092265 U CN 204092265U CN 201420617651 U CN201420617651 U CN 201420617651U CN 204092265 U CN204092265 U CN 204092265U
- Authority
- CN
- China
- Prior art keywords
- adjusting device
- mobile adjusting
- wishbone
- screw
- slide block
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
Landscapes
- Surgical Instruments (AREA)
Abstract
A kind of animal spinal cord living imaging Orientation observation device, it is made up of base plate (1), the mobile adjusting device in left side (2), the mobile adjusting device in right side (3) and implanted observation window (4); The mobile adjusting device in left side (2), the mobile adjusting device in right side (3) are fixed on base plate (1) by screw, and implanted observation window (4) plugs together location by the rectangular preiection of the horizontal location slide block on the rectangular opening on its V-type frame and left side mobile adjusting device (2), the mobile adjusting device in right side (3).This utility model can either be conducive to the installation operation of implanted observation window, makes the stability of spinal column better, can be conducive to again the dynamic observation of postoperative two-photon spinal cord imaging.Left and right side moves the internal fixtion state that the horizontal location slide block in adjusting device can not only provide horizontal direction stable to the operation of implantation device, gives stable extenal fixation and support when more can observe after surgery.
Description
Technical field:
This utility model relates to a kind of animal spinal cord living imaging Orientation observation device, it is used in the experimentation in neurobiology field, the installation operation of implanted observation window can be carried out to animal, and the change that live body dynamically observes spinal nervous tissue and blood vessel thereof can be carried out for a long time, to the particularly physiology of spinal cord injury and the pathological change of research nervus centralis, and after spinal cord injury, neural renewable reservoir has very large value.
Background technology:
In the experimentation in neurobiology field, traditional animal spinal cord living imaging means are the vertebral plate of an excision animal sections thus exposing spinal cord, after using whole body suspension method or local Clamping stabilizing spine, fluorescence microscope or Two Photon Fluorescence is used to carry out the dynamic imaging of live body to the animal spinal cord exposed.Imaging terminates rear layer-by-layer suture muscle and skin, cuts skin and muscle more next time, expose original segments of spinal cord with this repeatedly when observing.But this process can not last long, anesthesia and repeat surgery not only can bring the harm of whole body function to animal for a long time, more can improve animal infected risk, increase inflammatory reaction, cause tissue injury, initiation animal is more painful, is unfavorable for further observing and experimentation.And the operation of not seeking unity of standard at present is fixing and clinical follow external fixer, causes operation technique complicated, the difficulty that postoperative imaging is fixing.
Utility model content:
1, object: the purpose of this utility model is to provide a kind of animal spinal cord living imaging Orientation observation device.It can either be conducive to the installation operation of implanted observation window, makes the stability of spinal column better, can be conducive to again the dynamic observation of postoperative two-photon spinal cord imaging.The internal fixtion state that the horizontal location slide block of living imaging external fixer provide horizontal direction stable can not only to the operation of implantation device, gives stable extenal fixation and supports when more can observe after surgery.
2, technical scheme: the technical solution of the utility model is: a kind of animal spinal cord living imaging Orientation observation device, it is made up of base plate (1), the mobile adjusting device in left side (2), the mobile adjusting device in right side (3) and implanted observation window (4); Position annexation between them is: the mobile adjusting device in left side (2), the mobile adjusting device in right side (3) are fixed on base plate (1) by screw, and implanted observation window (4) plugs together location by the rectangular preiection of the horizontal location slide block on the rectangular opening on its V-type frame and left side mobile adjusting device (2), the mobile adjusting device in right side (3).
Described base plate (1) is one piece of rectangle plate, its be each side provided with two install screwed holes for the mobile adjusting device in left side (2), the mobile adjusting device in right side (3) fixing.
The mobile adjusting device in described left side (2) is made up of dovetail groove base (2-1), horizontal location slide block (2-2) and dog screw (2-3); Position annexation between them is: this dovetail groove base (2-1) is in swallow-tail form in the horizontal direction, can make horizontal location slide block (2-2) bilateral reciprocation being within it similarly swallow-tail form; When sliding into target location, screw dog screw (2-3), dog screw withstands dovetail groove base (2-1), reaches the object preventing dovetail groove base (2-1) and horizontal location slide block (2-2) relative sliding.The rectangular shape of this dovetail groove base (2-1) profile, horizontal direction is provided with swallow tail shaped slot, and vertical direction is rectangular block.This horizontal location slide block (2-2) is that rectangular slab materials and parts process, and the screw that it is arranged is used for and the connection of dog screw (2-3) and location, and end is processed into rectangular preiection and is convenient to plug together location with the rectangular opening on wishbone; Afterbody is processed into dovetail form and is convenient to slide in dovetail groove base (2-1).This dog screw (2-3) is commercial connector.
The mobile adjusting device in described right side (3) is identical with left side mobile adjusting device (2) structure, and specular is installed;
Described implanted observation window (4) is made up of left side wishbone (4-1), right side wishbone (4-2), strip pad (4-3), rectangle coverslip (4-4) and mounting screw (4-5); Position annexation between them is: left side wishbone (4-1) and right side wishbone (4-2) are respectively provided with two screwed holes, after position is fixing, strip pad (4-3) is vertically placed on left and right sides wishbone screwed hole, by mounting screw (4-5), strip pad (4-3) and left and right sides wishbone is fixed.In the middle of two strip pads (4-3), rectangle coverslip (4-4) can be inserted.Distance between left side wishbone (4-1) and right side wishbone (4-2) can be regulated by the adjustment hole of strip pad (4-3).
Operation principle of the present utility model and flow process brief introduction as follows:
Operation principle of the present utility model is: by living imaging external fixer and implanted observation window, realize the object of installing animal spinal cord implanted observation window, and then postoperative use Two Photon Fluorescence carries out living imaging to spinal cord, thus complete the operation of vivo observation spinal cord neural cell dynamic change.
Workflow of the present utility model is: for further illustrating the technical solution of the utility model, is now briefly described as follows by the operative process that this utility model carries out:
(1) operation installed by implanted observation window: by Animal Anesthesia, back uses iodophor disinfection after shaving hair, is placed on base plate, and skin is cut in projection place of edge back part of animal central authorities breast 13-waist 2, cleaning breast 13-waist 2 vertebral plate Surrounding muscles and soft tissue, appear breast 13-waist 2 vertebral plate.
(2) both sides horizontal location slide block is stretched into wishbone slotted eye, namely both sides wishbone load level positioning sliding block, horizontal slip horizontal location slide block, makes both sides wishbone clamping breast 13-waist 2 vertebral body.
(3) use micro-shears by waist 1 vertebrae plate resection, fully expose waist 1 segments of spinal cord.
(4) strip pad is positioned over above both sides wishbone and spinal cord, tightens four screws.
(5) at the biological silicone rubber of spinal cord surface coverage one deck, by rectangle coverslip covering resin.Use rapid-curing cutback seccotine and dental base acrylic resin powder fix and close implanted observation window, after seccotine and dental base acrylic resin powder solidify, both sides horizontal location slide block is shifted out both sides V-type frame slotted eye, skin suture has been performed the operation.
(6) Two Photon Fluorescence is observed fixing: by Animal Anesthesia, is positioned over living imaging external fixer central authorities.By both sides horizontal location slide block horizontal slip, stretch into wishbone both sides slotted eye, fixing implanted observation window and animal spinal column.
(7) Two Photon Fluorescence is used to carry out live body neuroimaging to animal spinal cord.
(8) observed, both sides horizontal location slide block has been shifted out both sides V-type frame slotted eye.
This utility model has the following advantages:
1, implanted observation window is conducive to using Two Photon Fluorescence to carry out long-term live body to animal spinal cord and dynamically observe, and avoids the problem that repeat surgery brings.
2, external fixer is not only conducive to the carrying out that operation installed by implanted observation window, is more conducive to the fixing of postoperative animal spinal cord observation.
3, this system is applied widely, can be used for the large-scale experiment animals such as rat, rabbit, Canis familiaris L., monkey after change size.
Accompanying drawing explanation
Fig. 1 is the structure chart of this animal spinal cord living imaging Orientation observation device
Fig. 2 a is the structure chart of implanted observation window
Fig. 2 b is wishbone schematic diagram
Fig. 2 c is strip pad schematic diagram
Fig. 2 d is rectangle coverslip schematic diagram
Fig. 3 a is the structure chart of mobile adjusting device
Fig. 3 b is horizontal location slide block schematic diagram
Fig. 3 c is dovetail groove base schematic diagram
Fig. 4 is base plate schematic diagram
In figure, sequence number is described as follows:
1, base plate; 2, the mobile adjusting device in left side; 3, the mobile adjusting device in right side; 4, implanted observation window;
2-1, dovetail groove base; 2-2, horizontal location slide block; 2-3, dog screw;
4-1, left side wishbone; 4-2, right side wishbone; 4-3, strip pad; 4-4, rectangle coverslip; 4-5, mounting screw.
Detailed description of the invention
See Fig. 1-Fig. 4, the technical solution of the utility model is: a kind of animal spinal cord living imaging Orientation observation device, and it is made up of base plate (1), the mobile adjusting device in left side (2), the mobile adjusting device in right side (3), implanted observation window (4); Position annexation between them is: the mobile adjusting device in left side (2), the mobile adjusting device in right side (3) are fixed on base plate (1) by screw, and implanted observation window (4) plugs together location by the rectangular preiection on rectangular opening on it and left side mobile adjusting device (2), the mobile adjusting device in right side (3).
Described installation (1) is one piece of rectangle plate, its each side arrange two install screwed holes for the mobile adjusting device in left side (2), the mobile adjusting device in right side (3) fixing.
The mobile adjusting device in described left side (2) is made up of dovetail groove base (2-1), horizontal location slide block (2-2) and dog screw (2-3); Position annexation between them is: this dovetail groove base (2-1) is in swallow-tail form in the horizontal direction, can make horizontal location slide block (2-2) bilateral reciprocation being within it similarly swallow-tail form; When sliding into target location, screw dog screw (2-3), dog screw withstands dovetail groove base (2-1), reaches the object preventing dovetail groove base (2-1) and horizontal location slide block (2-2) relative sliding.The rectangular shape of this dovetail groove base (2-1) profile, horizontal direction is provided with swallow tail shaped slot, and vertical direction is rectangular block.This horizontal location slide block (2-2) is that rectangular slab materials and parts process, and the screw that it is arranged is used for and the connection of dog screw (2-3) and location, and end is processed into rectangular preiection and is convenient to plug together location with the rectangular opening on wishbone; Afterbody is processed into dovetail form and is convenient to slide in dovetail groove base (2-1).This dog screw (2-3) is commercial connector.
The mobile adjusting device in described right side (3) is identical with left side mobile adjusting device (2) structure, and mirror image is installed;
Described implanted experiment porch (4) is made up of left side wishbone (4-1), right side wishbone (4-2), strip pad (4-3), rectangle coverslip (4-4) and mounting screw (4-5); Position annexation between them is: left side wishbone (4-1) and right side wishbone (4-2) respectively have two screwed holes, after position is fixing, strip pad (4-3) is vertically placed on left and right sides wishbone screwed hole, by mounting screw (4-5), strip pad (4-3) and left and right sides wishbone is fixed.In the middle of two strip pads (4-3), rectangle coverslip (4-4) can be inserted.Distance between left side wishbone (4-1) and right side wishbone (4-2) can be regulated by the adjustment hole of strip pad (4-3).
Be schematic with reference to accompanying drawing to description of the present utility model above, do not have restricted, those skilled in the art should be understood that, in reality is implemented, in this utility model, the shape of each component and layout type some all may occur change; And under enlightenment of the present utility model, other staff also can make the design similar with this utility model or this utility model is modified and certain component be equal to replacement.Special needs to be pointed out is, only otherwise depart from design aim of the present utility model, all apparent changes and have equivalent similar Design of replacing, are all included within protection domain of the present utility model.
Claims (1)
1. an animal spinal cord living imaging Orientation observation device, is characterized in that: it is made up of base plate (1), the mobile adjusting device in left side (2), the mobile adjusting device in right side (3) and implanted observation window (4); The mobile adjusting device in left side (2), the mobile adjusting device in right side (3) are fixed on base plate (1) by screw, and implanted observation window (4) plugs together location by the rectangular preiection of the horizontal location slide block on the rectangular opening on its V-type frame and left side mobile adjusting device (2), the mobile adjusting device in right side (3);
Described base plate (1) is one piece of rectangle plate, its be each side provided with two install screwed holes for the mobile adjusting device in left side (2), the mobile adjusting device in right side (3) fixing;
The mobile adjusting device in described left side (2) is made up of dovetail groove base (2-1), horizontal location slide block (2-2) and dog screw (2-3); This dovetail groove base (2-1) is in swallow-tail form in the horizontal direction, makes horizontal location slide block (2-2) bilateral reciprocation being within it similarly swallow-tail form; When sliding into target location, screw dog screw (2-3), dog screw withstands dovetail groove base (2-1); The rectangular shape of this dovetail groove base (2-1) profile, horizontal direction is provided with swallow tail shaped slot, and vertical direction is rectangular block; This horizontal location slide block (2-2) is that rectangular slab materials and parts process, the screw that it is arranged is used for and the connection of dog screw (2-3) and location, end is processed into rectangular preiection and is convenient to plug together location with the rectangular opening on wishbone, and afterbody is processed into dovetail form and is convenient to slide in dovetail groove base (2-1);
The mobile adjusting device in described right side (3) is identical with left side mobile adjusting device (2) structure, and specular is installed;
Described implanted observation window (4) is made up of left side wishbone (4-1), right side wishbone (4-2), strip pad (4-3), rectangle coverslip (4-4) and mounting screw (4-5); Left side wishbone (4-1) and right side wishbone (4-2) are respectively provided with two screwed holes, after position is fixing, strip pad (4-3) is vertically placed on left and right sides wishbone screwed hole, by mounting screw (4-5), strip pad (4-3) and left and right sides wishbone is fixed; In the middle of two strip pads (4-3), insert rectangle coverslip (4-4), the distance between left side wishbone (4-1) and right side wishbone (4-2) is then regulated by the adjustment hole of strip pad (4-3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420617651.0U CN204092265U (en) | 2014-10-23 | 2014-10-23 | A kind of animal spinal cord living imaging Orientation observation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420617651.0U CN204092265U (en) | 2014-10-23 | 2014-10-23 | A kind of animal spinal cord living imaging Orientation observation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204092265U true CN204092265U (en) | 2015-01-14 |
Family
ID=52260736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420617651.0U Withdrawn - After Issue CN204092265U (en) | 2014-10-23 | 2014-10-23 | A kind of animal spinal cord living imaging Orientation observation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204092265U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104287865A (en) * | 2014-10-23 | 2015-01-21 | 中国人民解放军总医院 | Fixing and observing device for living imaging of animal spinal cords |
| CN104873289A (en) * | 2015-02-06 | 2015-09-02 | 张金辉 | Animal spinal fixator |
| CN107796695A (en) * | 2017-10-17 | 2018-03-13 | 首都医科大学附属北京世纪坛医院 | For biomaterial tensile test fixture and grip method |
| CN107802372A (en) * | 2017-10-19 | 2018-03-16 | 中国科学院深圳先进技术研究院 | A kind of animal eyeball fixing device for photoacoustic imaging system |
-
2014
- 2014-10-23 CN CN201420617651.0U patent/CN204092265U/en not_active Withdrawn - After Issue
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104287865A (en) * | 2014-10-23 | 2015-01-21 | 中国人民解放军总医院 | Fixing and observing device for living imaging of animal spinal cords |
| CN104873289A (en) * | 2015-02-06 | 2015-09-02 | 张金辉 | Animal spinal fixator |
| CN104873289B (en) * | 2015-02-06 | 2017-05-31 | 张金辉 | animal spinal fixing device |
| CN107796695A (en) * | 2017-10-17 | 2018-03-13 | 首都医科大学附属北京世纪坛医院 | For biomaterial tensile test fixture and grip method |
| CN107796695B (en) * | 2017-10-17 | 2019-08-30 | 首都医科大学附属北京世纪坛医院 | For biomaterial tensile test fixture and grip method |
| CN107802372A (en) * | 2017-10-19 | 2018-03-16 | 中国科学院深圳先进技术研究院 | A kind of animal eyeball fixing device for photoacoustic imaging system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204092265U (en) | A kind of animal spinal cord living imaging Orientation observation device | |
| ES2550238T3 (en) | Cell populations that co-express CD49c and CD90 | |
| CN104287865A (en) | Fixing and observing device for living imaging of animal spinal cords | |
| Fernández-García et al. | M2 cortex-dorsolateral striatum stimulation reverses motor symptoms and synaptic deficits in Huntington’s disease | |
| WO2014008369A1 (en) | Radiolucent cranial implants for neural applications | |
| Gulati et al. | Multi-layer cortical Ca2+ imaging in freely moving mice with prism probes and miniaturized fluorescence microscopy | |
| CN206473428U (en) | A kind of integrated lighting temperature control micro- stereotaxic instrument of improvement | |
| CN204501481U (en) | A kind of sizable in-vitro eyeball fixture | |
| Madden et al. | Brain tumor imaging: live imaging of glioma by two-photon microscopy | |
| Overton et al. | Improved methods for acrylic-free implants in nonhuman primates for neuroscience research | |
| CN111419181A (en) | Device for long-time living body imaging of small animal and using method thereof | |
| CN102028546A (en) | Carp electrode transforation implantation brain stereotaxic method | |
| CN104323869B (en) | Be applicable to the constant operation table accessory that intracranial implants rat | |
| US20210282716A1 (en) | Awake-Animal Imaging Restrainer System for Animal Models of Brain Diseases | |
| CN204744104U (en) | Fixed formation of image of animal back of body root neuromere - spinal cord and oppression damage composite set | |
| CN209499967U (en) | Mouse for studying the pernicious biological property relationship of breast cancer cell observes device | |
| CN104771145A (en) | Animal dorsal root ganglion-spinal cord fixed imaging and compression injury combining device | |
| Maiorino et al. | Longitudinal intravital imaging through clear silicone windows | |
| Acharya et al. | Stem cell transplantation strategies for the restoration of cognitive dysfunction caused by cranial radiotherapy | |
| Cisneros et al. | Evaluation of the microgrinding procedure for the microscopic analysis of temporal bones | |
| Liu et al. | Spinal pathomorphological changes in the breeding giant salamander juveniles | |
| Lin et al. | Imaging neural activity in the primary somatosensory cortex using Thy1-GCaMP6s transgenic mice | |
| Trautmann et al. | Design of an implantable artificial dural window for chronic two-photon optical imaging in non-human primates | |
| RU2375012C1 (en) | Device for laboratory animal fixation | |
| Geraldo et al. | Dynamic immunotherapy study in brain tumor-bearing mice |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20150114 Effective date of abandoning: 20161012 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |