CN204744104U - Fixed formation of image of animal back of body root neuromere - spinal cord and oppression damage composite set - Google Patents
Fixed formation of image of animal back of body root neuromere - spinal cord and oppression damage composite set Download PDFInfo
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- CN204744104U CN204744104U CN201520224450.9U CN201520224450U CN204744104U CN 204744104 U CN204744104 U CN 204744104U CN 201520224450 U CN201520224450 U CN 201520224450U CN 204744104 U CN204744104 U CN 204744104U
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Abstract
The utility model relates to a composite set of fixed formation of image of animal back of the body root neuromere - spinal cord and oppression damage, composite set fixes image device and injection of micro - fluorescent dyes and spinal cord oppression damage integrated device including back of the body root neuromere - spinal cord, and wherein the animal carries on the back root neuromere - spinal cord fixed image device (1) including horizontal slider guide rail (1 -1), fixed part (1 -2), slide bar (1 -3), backbone fixation clamp (1 -4), part (2 -6) is oppressed with spinal cord oppression damage integrated device (2) including perpendicular - rotation regulation slide bar (2 -3), horizontal adjustment Assembly (2 -4), microinjection part (2 -5), spinal cord to the injection of micro - fluorescent dyes. The utility model discloses a composite set is with multiple functions collection in an organic whole, and is easy and simple to handle, switches at any time, conveniently makes spinal cord injury animal models in various degree to utilize two -photon microscope real time kinematic to observe the pathology physiology change process of back of the body root neuromere and spinal cord injury after reaching fluorescent dyes microinjection.
Description
Technical field
This utility model discloses a kind of animal back root neural section-spinal cord fixed imaging and compression device, and it is used in the experimentation in neurobiology field.Device of the present utility model can carry out the two-photon dynamic imaging of neuron and nerve fiber to the single or multiple dorsal root ganglion of animal and segments of spinal cord; And the compressive spinal cord injury animal model that can be formed in various degree.Device of the present utility model to the pathophysiological change studying dorsal root ganglion and spinal cord injury, and has very large value to the foundation of spinal cord compression injury's animal model.
Background technology
In the experimentation in neurobiology field, traditional dorsal root ganglion and spinal cord formation method make tissue slice, this technology needs to put to death animal and organizes with taking-up, tissue slice and dyeing is done after fixing dorsal root ganglion and spinal cord, then use fluorescence microscope histopathologic change, this method in real time, dynamically can not observe dorsal root ganglion and spinal nerves pathophysiological change process in vivo; Traditional NYU spinal cord compression injury device needs conputer controlled, and complicated operation involves great expense, and does not have supporting spinal fixation system.Publication number is that the utility application of CH104287865A discloses a kind of animal spinal cord living imaging Orientation observation device, and it is made up of base plate, the mobile adjusting device in left side, the mobile adjusting device in right side and implanted observation window; The mobile adjusting device in left side, the mobile adjusting device in right side are fixed on base plate by screw, implanted observation window plugs together location by the rectangular preiection of the horizontal location slide block in the mobile adjusting device of the rectangular opening on its V-type frame and left side, the mobile adjusting device in right side, this utility model can either be conducive to the installation operation of implanted observation window, make the stability of spinal column better, the dynamic observation of postoperative two-photon spinal cord imaging can be conducive to again.Left and right side moves the internal fixtion state that the horizontal location slide block in adjusting device can not only provide horizontal direction stable to the operation of implantation device, gives stable extenal fixation and support when more can observe after surgery.But which provides only the Orientation observation device of spinal cord, cannot dorsal root ganglion be observed, more can not complete the functions such as injection, imaging, damage modeling.In order to overcome above-mentioned technological deficiency, the utility model discloses a kind of animal back root neural section-spinal cord fixed imaging device and repressive damaging device and method for building up thereof, animal spinal column can be stablized very simply, microscopic fluorescence Dye Injections device is used single or multiple dorsal root ganglion and segments of spinal cord to be carried out to the microinjection of fluorescent dye, and by the air pump device of external conputer controlled, thus the accurate control to microinjection amount can be reached.Then Two Photon Fluorescence is used to carry out dynamic imaging to dorsal root ganglion and segments of spinal cord.Can provide the spinal compression damage dynamics of Different Weight, manufacture spinal cord injury model in various degree, microscopic fluorescence Dye Injections and compression device are integrated in one, easy and simple to handle, switch at any time, convenient spinal cord injury of observing in various degree simultaneously.
Utility model content
This utility model provides the combination unit of a kind of animal back root neural section-spinal cord fixed imaging and compression, it is characterized in that, described combination unit comprises dorsal root ganglion-spinal cord fixed imaging device (1) and microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus (2) and base plate (2-1), and dorsal root ganglion-spinal cord fixed imaging device (1) and microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus (2) are positioned at (2-1) on base plate.
Further, described dorsal root ganglion-spinal cord fixed imaging device comprises the fixture of at least two groups for fixing spinal cord, described fixture comprises spinal cord fixation clamp (1-4) and slide bar (1-3), wherein spinal cord fixation clamp (1-4) is fixed on slide bar, described spinal cord fixation clamp (1-4) comprises outside slip clamp (1-4-1) and inner slide folder (1-4-2), outside slip clamp wherein can upwards be mentioned, thus inside rotation spinal column, exposes lateral root dorsal ganglion to inward side.
Further, one end of described slide bar has screw thread, described spinal cord fixation clamp (1-4) is fixed on by the hole on it on screw thread of slide bar (1-3), and can be that axle rotates with slide bar, the hole of outside slip clamp (1-4-1) is wherein long and narrow strip, makes outside slip clamp except rotating and can move in the plane of slip clamp around slide bar, slip clamp has snap close, can be fixed by snap close after slip clamp is adjusted to the right place.
Further, described dorsal root ganglion-spinal cord fixed imaging device also comprises for the L-type slide bar fixed block (1-2-2) of fixing spinal cord fixation clamp and cross sliding clock (1-2-1) and cross sliding clock guide rail (1-1), described L-type slide bar fixed block (1-2-2) is fixed on cross sliding clock (1-2-1), and can move horizontally in cross sliding clock guide rail (1-1) along with cross sliding clock.
Further, described microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus comprise microscopic fluorescence Dye Injections device and spinal cord compression injury's device.
Further, described microscopic fluorescence Dye Injections device comprises the microinjector to dorsal root ganglion injecting virus-dyestuff, and preferably, described microinjector is connected with conputer controlled air pump device and carries out injection operation.
Further, described spinal cord compression injury's device comprises spinal compression post and weight tray, and weight tray is positioned at the top of spinal compression post, and the bottom of spinal compression post is tapered.
Further, described microscopic fluorescence Dye Injections device and spinal cord compression injury's device with vertical-rotate and regulate slide bar to be connected, vertically described-rotation regulates slide bar to comprise vertical adjusting slide bar bottom and rotate and regulates slide bar top, vertical adjusting slide bar bottom portion is fixed on base plate, and rotation regulates slide bar top and injection device are connected with damaging device and by rotating, injection device and damaging device can be moved to operating position.
Further, also comprise Two Photon Fluorescence, microscope is positioned at above spinal cord fixation clamp.
Further, cross sliding clock guide rail has dovetail groove, cross sliding clock bottom has the projection matched with dovetail groove, and this projection embeds in dovetail groove and also can slide along the direction of dovetail groove.
Further, described base plate has animal placing trough.
This utility model has the following advantages:
1, the strong fix to animal back root neural section and spinal cord is realized, by slip cross sliding clock in conjunction with the stable fixing single segmental of spinal column fixation clamp or multistage spinal column.After vertebral plate and pedicle of vertebral arch resection operation, by above putting forward the outside slip clamp of spinal column fixation clamp, spinal column can be rotated thus fully exposes outside dorsal root ganglion in direction, inner side, and then Two Photon Fluorescence can be used to carry out dynamic imaging to the neuron of dorsal root ganglion and spinal cord and nerve fiber simultaneously, overcome the defect simultaneously can not carrying out imaging to the neuron of dorsal root ganglion and spinal cord.
2, by the air pump device of external conputer controlled, minimum gas is pumped into the microinjector that air pump adapter connects, thus reach the accurate control to microinjection amount.
3, microscopic fluorescence Dye Injections is switched integrated with spinal cord compression injury, slide bar top is regulated by rotating, timely switching microscopic fluorescence Dye Injections and spinal cord compression injury, realize setting up spinal compression damage model to animal, by increase and the minimizing of the upper counterweight quantity of weight tray (2-6-2), the convenient spinal compression damage model set up in various degree.
4, the adjustable angle of microscopic fluorescence Dye Injections device on horizontal adjustment slide rail, is beneficial to control injection angles, avoids the position of per injection during manual operation inconsistent.
5, the hold-down nut of vertical adjusting slide bar bottom is regulated, slide bar top can be regulated by lifting rotation, distance between weight tray and animal spinal cord is adjusted, facilitates different building shape animal to use, can be used for the large-scale experiment animals such as mice, rat, rabbit, Canis familiaris L., pig, monkey.
6, adjust the spacing between spinal cord fixation kit, facilitate different building shape animal to use, can be used for the large-scale experiment animals such as mice, rat, rabbit, Canis familiaris L., pig, monkey.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of animal back root neural section-spinal cord fixed imaging device 1 and compression device 2.
Fig. 2 is the schematic diagram of dorsal root ganglion-spinal cord fixed imaging device 1.
Fig. 3 is the schematic diagram of the cross sliding clock guide rail 1-1 in dorsal root ganglion-spinal cord fixed imaging device 1.
Fig. 4 is the schematic diagram of cross sliding clock 1-2-1 in dorsal root ganglion-spinal cord fixed imaging device 1 and L-type slide bar fixed block 1-2-2.
Fig. 5 is the 1-4 schematic diagram of slide bar 1-3 in dorsal root ganglion-spinal cord fixed imaging device and spinal column fixation clamp, and described spinal column fixation clamp comprises outside slip clamp 1-4-1 and inner side fixation clamp 1-4-2.
Fig. 6 is the schematic diagram of microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus 2.
Fig. 7 is the schematic diagram of end 2-1 plate in microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus 2 and animal placing trough 2-2.
Fig. 8 be microscopic fluorescence Dye Injections with in spinal cord compression injury's integrated apparatus 2 vertical-rotate the schematic diagram regulating slide bar 2-3, wherein vertically-rotate and regulate slide bar 2-3-1 to comprise vertical adjusting slide bar bottom and rotate to regulate slide bar top 2-3-2.
Fig. 9 is the schematic diagram of horizontal adjustment slide rail in microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus and microinjector pallet and spinal compression lever bracket dish.
Figure 10 is the schematic diagram of microinjector in microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus and air pump adapter.
Figure 11 is the schematic diagram of spinal compression bar in microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus and weight tray.
Reference numeral:
1. dorsal root ganglion-spinal cord fixed imaging device, 1-1. cross sliding clock guide rail, 1-2. fixed part, 1-2-1. cross sliding clock, 1-2-2.L type slide bar fixed block, 1-3. slide bar, 1-4. spinal column fixation clamp, slip clamp outside 1-4-1., 1-4-2. inner slide is pressed from both sides.
2. microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus, 2-1. base plate, 2-2. animal placing trough, 2-3. is vertical-and rotate and regulate slide bar, 2-3-1. vertical adjusting slide bar bottom, 2-3-2. rotates and regulates slide bar top, 2-4. horizontal adjustment parts, 2-4-1. horizontal adjustment slide rail, 2-4-2. microinjector pallet, 2-4-3. spinal compression lever bracket dish, 2-5. microinjection parts, 2-5-1. microinjector, 2-5-2. air pump adapter, 2-6. spinal compression parts, 2-6-1. spinal compression post, 2-6-2. scale pan.
Detailed description of the invention
Concrete sees Fig. 1-11, and combination unit of the present utility model comprises dorsal root ganglion-spinal cord fixed imaging device 1, microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus 2.Dorsal root ganglion-spinal cord fixed imaging device is by regulating cross sliding clock 1-2-1 and L-type slide bar fixed block 1-2-2, reach and 1-4 is fixed to the clamp of the single or multiple sections of animal spinal column, then single phase or multistage vertebral plate and pedicle of vertebral arch resection operation is carried out, carry on the outside slip clamp 1-4-1 of spinal column fixation clamp 1-4, reach to inward side to rotating spinal column thus the object of exposure outside dorsal root ganglion, the postoperative microinjector 2-5-1 connected to air pump adapter 2-5-2 by conputer controlled air pump device carries out the microinjection of the dyestuffs such as adeno-associated virus-green fluorescent protein to dorsal root ganglion, in the neuron transfection of dorsal root ganglion after green fluorescent protein, Two Photon Fluorescence is used to carry out living imaging to the nerve fiber that dorsal root ganglion sends with it, observe the physiological structure of dorsal root ganglion neurons and nerve fiber.By regulating rotating rod top 2-3-2 to horizontally rotate on spinal cord by spinal compression bar 2-6-1, by the counterweight foundation compressive spinal cord injury model in various degree of Different Weight on weight tray 2-6-2.Dorsal root ganglion neurons and the dynamic pathological change of nerve fiber after spinal cord injury is observed again by Two Photon Fluorescence
Another aspect of this utility model is to provide the using method of a kind of animal back root neural section-spinal cord fixed imaging device and compression device:
Operation principle of the present utility model is: by dorsal root ganglion-spinal cord fixed imaging device 1, realize the strong fix to living animal dorsal root ganglion and spinal cord, by above carrying the outside slip clamp 1-4-1 of spinal column fixation clamp 1-4, reach to inward side to rotating spinal column thus the object of exposure outside dorsal root ganglion.By microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus 2, dorsal root ganglion is carried out to the micro-fixed-point injection of fluorescin or dyestuff, and then use Two Photon Fluorescence to carry out living imaging to the neuron of dorsal root ganglion-spinal cord and nerve fiber, thus complete the operation that live body continues to observe spinal cord neural cell dynamic change.Regulate slide bar 2-3-2 by rotating, spinal cord compression injury's device is rotated above spinal cord, realize the spinal compression damage model setting up animal, complete the operation of spinal compression model in various degree.
Workflow of the present utility model is: for further illustrating the technical solution of the utility model, is now described below by the operative process that this utility model carries out:
(1) vertebral plate and pedicle of vertebral arch resection operation: by Animal Anesthesia, back uses iodophor disinfection after shaving hair, being put in animal is seated in animal placing trough 2-2, skin is cut along projection place of back part of animal central authorities waist 1-waist 3, cleaning waist 1-waist 3 vertebral plate Surrounding muscles and soft tissue, appear waist 1-waist 3 vertebral plate.By the position and the distance that regulate cross sliding clock 1-2-1 and L-type slide bar fixed block 1-2-2 to regulate both sides spinal column fixation clamp 1-4, use both sides spinal column fixation clamp (1-4) to carry out clamp to waist 1 and waist 3 vertebral body respectively to fix, use micro-shears by waist 2 vertebrae plate resection, abundant exposure waist 2 segments of spinal cord, use micro-shears waist 2 pedicle of vertebral arch to be excised further, appear waist 2 dorsal root ganglion.Carrying on the outside slip clamp 1-4-1 of spinal column fixation clamp 1-4, reaching to inward side to rotating spinal column thus the abundant object exposing outside dorsal root ganglion.
(2) the dyestuff microinjection such as adeno-associated virus-green fluorescent protein: pump into minimum gas to the microinjector 2-5-1 that air pump adapter 2-5-2 connects by using the air pump device of conputer controlled, adeno-associated virus-green fluorescent protein or dyestuff 2 μ l fixed-point injection are entered in waist 2 dorsal root ganglion, transfection is carried out to intraganglionic neuronal cell.
(3) Two Photon Fluorescence dynamic imaging: before each imaging, uses spinal column fixation clamp 1-4 to fix animal both sides spinal column, carries on the slip clamp 1-4-1 of outside, reaches to inward side to rotating spinal column thus the abundant object exposing outside dorsal root ganglion.Under the state that excitation wavelength is 920nm, use 25 times of Two Photon Fluorescence camera lenses to carry out dynamic imaging to the neuronal cell of waist 2 dorsal root ganglion and nerve fiber, observe the dynamic change of neuron and nerve fiber.
(4) foundation of spinal cord compression injury's model: when animal waist 1 and waist 3 vertebral body clamp fixing with vertebrae plate resection after, by regulating rotating rod top 2-3-2, spinal compression post 2-6-1 is placed on the spinal cord of exposure gently, by regulating the weight of compressing at scale pan 2-6-2 swing weight-adding, simultaneously according to different pressing times foundation spinal compression injured animal model in various degree.
(5) postoperative layer-by-layer suture muscle and skin, uses the generation that antibiotic prophylaxis infects.
As Fig. 1, shown in 7, the combination unit of animal back root neural section-spinal cord fixed imaging of the present utility model and microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus, comprise animal back root neural section-spinal cord fixed imaging device 1 and compression device 2, dorsal root ganglion-spinal cord fixed imaging device 1 and microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus 2 are positioned on base plate 2-1, base plate 2-1 has animal placing trough 2-2, dorsal root ganglion-spinal cord fixed imaging device 1 is positioned at the both sides of base plate 2-1 with microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus 2, wherein animal back root neural section-spinal cord fixed imaging device 1 comprises cross sliding clock guide rail 1-1, fixed part 1-2, slide bar 1-3, spinal column fixation clamp 1-4, spinal cord compression injury's integrated apparatus 2 comprises vertically-rotates adjustment slide bar 2-3, horizontal adjustment parts 2-4, microinjection parts 2-5, spinal compression parts 2-6.Wherein, animal placing trough is at dorsal root ganglion-spinal cord fixed imaging device 1 and between microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus 2.Cross sliding clock guide rail 1-1 is fixed on above base plate 2-1, and fixed part 1-2 is positioned on cross sliding clock guide rail, and can along cross sliding clock slide.Slide bar 1-3 is fixed on fixed part 1-2 by the perforation on fixed part 1-2, slide bar one end connects spinal column fixation clamp 1-4, can regulate the position of slide bar 1-3 that spinal column fixation clamp 1-4 is positioned at above animal placing trough 2-2.Vertically-rotating adjustment slide bar 2-3 is fixed on above base plate 2-1, horizontal adjustment parts 2-4 is positioned at vertically-rotates and regulates slide bar 2-3 upper end, be parallel to base plate 2-1 and regulate slide bar 2-3 to arrange perpendicular to vertical-rotation, and can with vertical-rotation adjustment slide bar 2-3 for axle rotates, one end of horizontal adjustment parts 2-4 is connected with spinal compression parts 2-6, and the other end is connected with microinjection parts 2-5.
As shown in Figure 2-5, fixed part 1-2 is positioned on cross sliding clock guide rail 1-1, and can along cross sliding clock slide.Slide bar 1-3 is fixed on fixed part 1-2 by the perforation on fixed part 1-2, slide bar one end connects spinal column fixation clamp 1-4, can regulate the position of slide bar 1-3 that spinal column fixation clamp 1-4 is positioned at above animal placing trough 2-2.Wherein cross sliding clock guide rail 1-1 has dovetail groove.Fixed part 1-2 comprises cross sliding clock 1-2-1 and L-type slide bar fixed block 1-2-2.Cross sliding clock 1-2-1 lower end has the projection corresponding with the dovetail groove of cross sliding clock guide rail 1-1, and this projection embeds in cross sliding clock guide rail 1-1, and cross sliding clock 1-2-1 is slided along cross sliding clock guide rail 1-1.Cross sliding clock 1-2-1 has a nut, when cross sliding clock 1-2-1 slip puts in place, can be fixed on cross sliding clock guide rail 1-1 with nut.L-type sliding dowel block 1-2-2 is fixed on cross sliding clock 1-2-1, and L-type sliding dowel block 1-2-2 has a perforation, and this perforation allows slide bar 1-3 to pass, and is fixed by the nut be positioned at above L-type sliding dowel block 1-2-2.One end of slide bar 1-3 has screw thread, and screw thread is fixed with vertebra fixation clamp 1-4.Vertebra fixation clamp 1-4 comprises outside slip clamp 1-4-1 and inner slide folder 1-4-2.Inner slide folder 1-4-2 is relative to outside slip clamp 1-4-1 distance L-type slide bar fixed block.Inner slide folder 1-4-1 and outside slip clamp 1-4-2 there is through hole, they are fixed on slide bar 1-3 by through hole, through hole wherein on the slip clamp 1-4-2 of outside becomes long and narrow bar shaped, makes outside slip clamp 1-4-2 can also carry out translation along the direction with long and narrow through hole.As shown in Fig. 6,8-11, wherein vertically-rotate adjustment slide bar 2-3 to be positioned on base plate 2-1.Vertically-rotation regulates slide bar 2-3 to comprise vertical adjusting slide bar bottom 2-3-1 and regulates slide bar top 2-3-2 with rotation, and rotation regulates slide bar top 2-3-2 to stretch up and down in vertical adjusting slide bar bottom 2-3-1 and to be parallel to base plate rotation.Vertical adjusting slide bar bottom 2-3-1 has nut, can be used in fixing rotation and regulate slide bar top 2-3-2.Horizontal adjustment parts 2-4 is positioned at vertically-rotates and regulates slide bar 2-3 upper end, be parallel to base plate 2-1 and regulate slide bar 2-3 to arrange perpendicular to vertical-rotation, and can with vertical-rotation adjustment slide bar 2-3 for axle rotates, one end of horizontal adjustment parts 2-4 is connected with spinal compression parts 2-6, and the other end is connected with microinjection parts 2-5.Wherein microinjection parts 2.5 comprise microinjector 2-5-1 and the air pump adapter 2-5-2 be attached thereto.Spinal compression parts 2-6 comprises spinal compression post 2-6-1 and scale pan 2-6-2.Scale pan 2-6-2 is positioned at the top of spinal compression post 2-6-1, and it can place counterweight.The bottom of spinal compression post 2-6-1 is tapered.Spinal compression post 2-6-1 and microinjector 2-5-1 is fixed by the chuck at horizontal adjustment parts 2-4 two ends, chuck has nut for regulating the position of fixing spinal compression post 2-6-1 and microinjector 2-5-1.2-3-2. rotates the position regulating the top on slide bar top to have nut purposes secured adjusted horizontal adjustment parts 2-4.
The above; be only detailed description of the invention of the present utility model; but protection domain of the present utility model is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that this utility model discloses; the change that can expect easily or replacement, all should be encompassed within protection domain of the present utility model.Therefore, protection domain of the present utility model should be as the criterion with the protection domain of described claim.
Claims (11)
1. the combination unit of animal back root neural section-spinal cord fixed imaging and compression, it is characterized in that, described combination unit comprises dorsal root ganglion-spinal cord fixed imaging device (1) and microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus (2), and dorsal root ganglion-spinal cord fixed imaging device (1) and microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus (2) are positioned on base plate (2-1).
2. the combination unit of animal back root neural section-spinal cord fixed imaging according to claim 1 and compression, it is characterized in that, described dorsal root ganglion-spinal cord fixed imaging device comprises the fixture of at least two groups for fixing spinal cord, described fixture comprises spinal cord fixation clamp (1-4) and slide bar (1-3), wherein spinal cord fixation clamp (1-4) is fixed on slide bar, , described spinal cord fixation clamp (1-4) comprises outside slip clamp (1-4-1) and inner slide folder (1-4-2), outside slip clamp wherein can upwards be mentioned, thus inside rotation spinal column, expose lateral root dorsal ganglion to inward side.
3. the animal back root neural section-spinal cord fixed imaging according to any one of claim 1-2 and the combination unit of compression, it is characterized in that, one end of described slide bar has screw thread, described spinal cord fixation clamp (1-4) is fixed on by the hole on it on screw thread of slide bar (1-3), and can be that axle rotates with slide bar, the hole of outside slip clamp (1-4-1) is wherein long and narrow strip, make outside slip clamp except rotating and can move in the plane of slip clamp around slide bar, slip clamp has snap close, can be fixed by snap close after slip clamp is adjusted to the right place.
4. the combination unit of animal back root neural section-spinal cord fixed imaging according to claim 3 and compression, it is characterized in that, described dorsal root ganglion-spinal cord fixed imaging device also comprises for the L-type slide bar fixed block (1-2-2) of fixing spinal cord fixation clamp and cross sliding clock (1-2-1) and cross sliding clock guide rail (1-1), described L-type slide bar fixed block (1-2-2) is fixed on cross sliding clock (1-2-1), and can move horizontally in cross sliding clock guide rail (1-1) along with cross sliding clock.
5. the combination unit of animal back root neural section-spinal cord fixed imaging according to claim 4 and compression, it is characterized in that, described microscopic fluorescence Dye Injections and spinal cord compression injury's integrated apparatus (2) comprise microscopic fluorescence Dye Injections device (2-5) and spinal cord compression injury's device (2-6).
6. the combination unit of animal back root neural section-spinal cord fixed imaging according to claim 5 and compression, it is characterized in that, described microscopic fluorescence Dye Injections device comprises the microinjector to dorsal root ganglion injecting virus-dyestuff, preferably, described microinjector (2-5-1) is connected with conputer controlled air pump device (2-5-2) and carries out injection operation.
7. the combination unit of animal back root neural section-spinal cord fixed imaging according to claim 6 and compression, it is characterized in that, described spinal cord compression injury's device comprises spinal compression post and weight tray, weight tray (2-6-2) is positioned at the top of spinal compression post (2-6-1), and the bottom of spinal compression post is tapered.
8. the combination unit of animal back root neural section-spinal cord fixed imaging according to claim 7 and compression, it is characterized in that, described microscopic fluorescence Dye Injections device (2-5) and spinal cord compression injury's device (2-6) with vertical-rotate and regulate slide bar (2-3) to be connected, vertically described-rotation regulates slide bar (2-3) to comprise vertical adjusting slide bar bottom (2-3-1) and rotate and regulates slide bar top (2-3-2), vertical adjusting slide bar bottom portion is fixed on base plate (2-1), and rotate and regulate slide bar top (2-3-2) to be connected with damaging device (2-6) with injection device (2-5) and can pass through to rotate injection device (2-5) and damaging device (2-6) are moved to operating position.
9. the combination unit of animal back root neural section-spinal cord fixed imaging according to claim 7 and compression, it is characterized in that, also comprise Two Photon Fluorescence, microscope is positioned at above spinal cord fixation clamp.
10. the combination unit of animal back root neural section-spinal cord fixed imaging according to claim 8 and compression, it is characterized in that, cross sliding clock guide rail (1-1) has dovetail groove, cross sliding clock (1-2-1) bottom has the projection matched with dovetail groove, and this projection embeds in dovetail groove and also can slide along the direction of dovetail groove.
The combination unit of 11. animal back root neural section-spinal cord fixed imagings according to claim 9 and compression, is characterized in that described base plate having animal placing trough (2-2).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520224450.9U CN204744104U (en) | 2015-04-14 | 2015-04-14 | Fixed formation of image of animal back of body root neuromere - spinal cord and oppression damage composite set |
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| CN201520224450.9U CN204744104U (en) | 2015-04-14 | 2015-04-14 | Fixed formation of image of animal back of body root neuromere - spinal cord and oppression damage composite set |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104771145A (en) * | 2015-04-14 | 2015-07-15 | 中国人民解放军总医院 | Animal dorsal root ganglion-spinal cord fixed imaging and compression injury combining device |
| CN106420101A (en) * | 2016-09-22 | 2017-02-22 | 西安医学院 | Injecting and fixing device for mouse |
| CN108784881A (en) * | 2018-03-28 | 2018-11-13 | 中国人民解放军总医院 | A kind of mouse kidney live body micro-imaging and tracer corollary system |
-
2015
- 2015-04-14 CN CN201520224450.9U patent/CN204744104U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104771145A (en) * | 2015-04-14 | 2015-07-15 | 中国人民解放军总医院 | Animal dorsal root ganglion-spinal cord fixed imaging and compression injury combining device |
| CN104771145B (en) * | 2015-04-14 | 2017-04-12 | 中国人民解放军总医院 | Animal dorsal root ganglion-spinal cord fixed imaging and compression injury combining device |
| CN106420101A (en) * | 2016-09-22 | 2017-02-22 | 西安医学院 | Injecting and fixing device for mouse |
| CN106420101B (en) * | 2016-09-22 | 2018-01-16 | 西安医学院 | A kind of injection and fixing device for mouse |
| CN108784881A (en) * | 2018-03-28 | 2018-11-13 | 中国人民解放军总医院 | A kind of mouse kidney live body micro-imaging and tracer corollary system |
| CN108784881B (en) * | 2018-03-28 | 2023-05-16 | 中国人民解放军总医院 | Mouse kidney living body microscopic imaging and tracing matching system |
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