CN112300904B - Graphene oxide hydrogel attached stem cell soaking device - Google Patents
Graphene oxide hydrogel attached stem cell soaking device Download PDFInfo
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- 238000002791 soaking Methods 0.000 title claims abstract description 75
- 239000000017 hydrogel Substances 0.000 title claims abstract description 43
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 26
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 229910021389 graphene Inorganic materials 0.000 title claims abstract description 25
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 21
- 239000010935 stainless steel Substances 0.000 claims abstract description 21
- 238000004113 cell culture Methods 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 3
- 239000010963 304 stainless steel Substances 0.000 claims description 2
- 229910000589 SAE 304 stainless steel Inorganic materials 0.000 claims description 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 abstract description 19
- 239000012620 biological material Substances 0.000 abstract description 17
- 239000000463 material Substances 0.000 abstract description 14
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 210000002421 cell wall Anatomy 0.000 abstract description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052782 aluminium Inorganic materials 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 description 7
- 239000011148 porous material Substances 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
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- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000037314 wound repair Effects 0.000 description 2
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 206010063560 Excessive granulation tissue Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
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- 238000010008 shearing Methods 0.000 description 1
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- 238000006467 substitution reaction Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Abstract
The invention discloses a graphene oxide hydrogel attached stem cell soaking device, which comprises a soaking device body; the soaking device body be equipped with the box, be equipped with soaking structure in the box, timing device and moving structure, soaking structure be cell culture soaking tank, be equipped with the soft network in the cell culture soaking tank, moving structure for setting up the movable rod around the soft network, the slide rail setting is on the cell culture soaking tank's cell wall, the position that the movable rod set up and the slide rail position mutually matches on the cell culture soaking tank, timing device be the timing controller. The invention adopts stainless steel, plastic, aluminum materials and the like to manufacture the timed intermittent soaking device for carrying the mesenchymal stem cells by the hydrogel materials, and solves the problem of carrying the mesenchymal stem cells by the hydrogel biological materials.
Description
Technical Field
The invention relates to the field of novel biological material preparation and experiments, in particular to a graphene oxide hydrogel attached stem cell soaking device.
Background
The mesenchymal stem cells are adult stem cells with self-renewal and multidirectional differentiation potential, and become hot spots for wound repair because the mesenchymal stem cells can differentiate into various mesenchymal tissues such as skin, blood vessels, skin appendages, bones, cartilage, fibrous tissue progenitor cells, bone marrow stroma and the like. Research shows that in the wound healing process, the induced BMSCs can differentiate into vascular endothelial-like cells, are closely related to the formation of small blood vessels in granulation tissues, participate in wound repair, and have better prospects compared with the traditional medicine treatment of mesenchymal stem cells. At present, few biological materials are available for carrying mesenchymal stem cells, the mesenchymal stem cells are limited to be applied to the treatment of chronic refractory wounds in a large range through external application, and hydrogel has congenital advantages as a biological material with a molecular structure similar to that of a natural extracellular matrix (ECM) in an organism. The hydrogel not only has good hydrophilicity and is beneficial to substance exchange, but also can provide good supporting and differentiating environment for cells.
However, in the process of carrying mesenchymal stem cells with new biological materials such as hydrogels, the details of the new biological materials need to be noted are relatively many, for example: the material soaking time in the culture medium, the fixation of the material in the soaking process, the time control of the soaking process and the like, and researches show that the intermittent soaking mode (namely, taking out the material after the culture medium is soaked for a fixed time and then putting the material into the material for soaking and repeatedly operating) is more beneficial to the growth of the stem cells in the culture medium containing the mesenchymal stem cells.
Disclosure of Invention
The technical problem to be solved by the invention is that the existing novel biological materials such as hydrogel have more detail problems in the process of carrying mesenchymal stem cells, for example: the soaking time of the material in the culture medium, the fixation of the material in the soaking process, the time control of the soaking process, and the like.
In order to solve the technical problems, the invention adopts the following technical means:
a graphene oxide hydrogel attached stem cell soaking device comprises a soaking device body; the soaking device body be equipped with the box, be equipped with soaking structure in the box, timing device and moving structure, soaking structure be cell culture soaking tank, be equipped with the soft network in the cell culture soaking tank, moving structure for setting up the movable rod around the soft network, the slide rail setting is on the cell culture soaking tank's cell wall, the position that the movable rod set up and the slide rail position mutually matches on the cell culture soaking tank, timing device be the timing controller.
The invention adopts stainless steel, plastic, aluminum materials and the like to manufacture the timed intermittent soaking device for carrying the mesenchymal stem cells by the hydrogel materials, and solves the problem of carrying the mesenchymal stem cells by the hydrogel biological materials.
Preferably, the invention further adopts the technical scheme that:
the top of the box body is provided with an opening, the opening is provided with a cover plate, the size of the cover plate is matched with the size of the opening, the bottom of the box body is provided with a box bottom, four walls are arranged around the box body, and the cover plate, the box bottom and the four walls enclose a soaking cavity.
The soft net on be equipped with the fixation clamp, the soft net passes through the mount to be fixed in the box, the quantity of fixation clamp is three, be equipped with the filtration pore on the soft net, the quantity of filtration pore is a plurality of, the even distribution of filtration pore on the soft net.
The timing controller is connected with the data line through a power line to control the soft net to lift on the sliding rail.
The cover plate is provided with an observation structure, the observation structure is an observation window, and the observation window is made of transparent glass.
The outer side of the box body is provided with a handle.
The cover plate, the box body, the cell culture soaking tank and the handle are all made of 304 stainless steel.
The number of the moving rods is four, and the number of the sliding rails is four, so that the sliding rails are matched with each other.
The soft net is a stainless steel net, the fixing clamp is a soft fixing clamp, the hydrogel is placed on the stainless steel net, and the hydrogel is fixed on the stainless steel net through the soft fixing clamp.
The soaking device body be equipped with supporting broken scissors, be by fixed screw with left main blade and right main blade connection fixed to all have a left handle and right handle respectively at the end, all fix right pair blade and left pair blade on main blade by right fixed screw and left fixed screw respectively in the upper end of main blade, all by the fixed screw fixation, make can free rotation, and pair blade is fixed respectively and keep the distance with main blade by left fixed screw and right fixed screw. When in use, the scissor heads are stretched into the container to contact sample tissues, and the scissor handles are opened and closed to cut the sample tissues.
The invention provides a timing-intermittent soaking device for attaching stem cells to graphene oxide hydrogel, which can enable biological materials such as hydrogel to become more convenient and accurate in the process of attaching the stem cells, remarkably reduce deviation of carrying the stem cells on the hydrogel biological materials caused by human factors, and enable the stem cells to grow better in the biological materials such as the graphene oxide hydrogel.
The benefit effect of the invention is as follows: the graphene oxide hydrogel attached stem cell timing-intermittent soaking device can realize full-automatic mesenchymal stem cell loading of hydrogel materials, standardized production is realized, production efficiency and standard are improved, and waste of manpower and materials is obviously reduced.
The invention has the advantages that:
1. on the basis of carrying mesenchymal stem cells on original artificial control graphene oxide hydrogel biological materials, the device realizes full-automatic standard production of mesenchymal stem cell hydrogel;
2. the process of carrying the mesenchymal stem cells by the hydrogel biological material is controllable, so that the mesenchymal stem cells on the biological material are distributed more uniformly;
3. the device reduces material cost and preparation time, improves work efficiency.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
Reference numerals illustrate: 1-a cover plate; 2-a box body; 3-timing controller; 4-soft net; 5-a cell culture soaking tank; 6-viewing window.
Description of the embodiments
The invention will be further illustrated with reference to the following examples.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Referring to fig. 1, the graphene oxide hydrogel attached stem cell soaking device of the present invention includes a soaking device body; the soaking device body is equipped with box 2, is equipped with soaking structure in the box 2, timing device and moving structure, soaking structure be cell culture soaking tank 5, be equipped with soft web 4 in the cell culture soaking tank 5, moving structure for setting up the movable rod around soft web 4, the slide rail sets up on cell culture soaking tank 5's cell wall, the position that the movable rod set up and the slide rail position on cell culture soaking tank 5 match each other, movable rod quantity be four, the quantity of slide rail is also four, the mutual matching, timing device be timing controller 3, box 2 top is equipped with the opening, the opening part is equipped with apron 1, the size of apron 1 and the size of opening mutually support, box 2 bottom is equipped with the bottom of the case, be equipped with the wall all around of box 2, apron 1, bottom of the case and wall enclose into soaking cavity, be equipped with the fixation clamp on soft web 4, the quantity of fixation clamp is three on box 2, be equipped with the filtration pore on soft web 4, the quantity of filtration pore is a plurality of evenly distributed on the flexible screen 4 is equipped with the window, the window glass window on the window glass that is equipped with on the window glass fiber optics, the window 3, the window is equipped with on the transparent glass-frame 6, the observation box is equipped with on the transparent glass-frame 6, the window is equipped with the transparent structure is on the observing window is 1, the window is equipped with the window glass-shaped structure is 6, the observing window is on the glass structure is equipped with the window is 6, the transparent material.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Referring to fig. 1, according to the graphene oxide hydrogel attached stem cell soaking device, the graphene oxide hydrogel attached stem cell timing-intermittent soaking device utilizes a time controller to realize intermittent soaking of graphene oxide hydrogel in a culture medium tank containing a certain cell number, the hydrogel is placed on a stainless steel net and fixed on the stainless steel net through a soft fixing clamp, a fixing program is input into the time controller, the stainless steel net moves up and down intermittently through a sliding rail, the hydrogel is soaked in the culture medium in a controllable intermittent manner, standardized operation of carrying mesenchymal stem cells by hydrogel biological materials is realized, influence of human factors is avoided, and efficiency of carrying mesenchymal stem cells by the hydrogel is greatly improved.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Referring to fig. 1, according to the graphene oxide hydrogel-attached stem cell soaking device, a left main blade and a right main blade are connected and fixed by a fixing screw, a left handle and a right handle are respectively arranged at the tail ends of the left main blade and the right auxiliary blade and the left auxiliary blade are respectively fixed on the main blade by the right fixing screw and the left fixing screw at the upper ends of the main blade, and are respectively fixed by the fixing screw, so that the graphene oxide hydrogel-attached stem cell soaking device can rotate freely, and the auxiliary blade is respectively fixed by the left fixing screw and the right fixing screw and has a certain distance from the main blade, so that the use and the shearing of the two blades are not affected.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Referring to fig. 1, the graphene oxide hydrogel attached stem cell soaking device of the present invention is a timing-intermittent soaking device for graphene oxide hydrogel attached stem cells, and is composed of 5 parts, respectively: the stainless steel cover plate 1 is arranged; a stainless steel box body 2 is arranged; a soaking timing controller 3; a biological material stainless steel fixed soft net 4; stainless steel cell culture soaking tank 5.
The timing-intermittent soaking device for the graphene oxide hydrogel attached stem cells is composed of a stainless steel cover and stainless steel groove bodies, grooves are formed in two sides of the groove bodies for carrying, a stainless steel filter screen and soft fixing clamps are arranged in the stainless steel groove bodies, and a time controller can be used for timing the stainless steel mesh to move up and down along a sliding rail so as to ensure that graphene oxide hydrogel biological materials on the stainless steel mesh can be completely soaked in a culture medium.
Since the above description is only specific embodiments of the present invention, the protection of the present invention is not limited thereto, and any equivalent changes or substitutions of technical features of the present invention will be apparent to those skilled in the art, and are included in the scope of the present invention.
Claims (6)
1. A graphene oxide hydrogel attached stem cell soaking device comprises a soaking device body; the method is characterized in that: the soaking device body is provided with a box body, a soaking structure, a timing device and a moving structure are arranged in the box body, the soaking structure is a cell culture soaking tank, a soft net is arranged in the cell culture soaking tank, the moving structure is a moving rod arranged around the soft net, a sliding rail is arranged on the wall of the cell culture soaking tank, the position of the moving rod is matched with the position of the sliding rail on the cell culture soaking tank, the timing device is a timing controller, a fixed clamp is arranged on the soft net, the soft net is fixed in the box body through a fixed frame, the number of the fixed clamps is three, a plurality of filtering holes are arranged on the soft net, the filtering holes are uniformly distributed on the soft net, the timing controller is connected with a control soft net through a power line and a data line to control the soft net to lift on the sliding rail, the soaking device body is provided with a matched crushing shear, the left main blade and the right main blade are connected and fixed through fixing screws, a left handle and a right handle are respectively arranged at the tail end of the soaking device body, the right auxiliary blade and the left auxiliary blade are respectively fixed on the main blade through the right fixing screws and the left fixing screws at the upper end of the main blade, the auxiliary blade is fixed through the fixing screws and can rotate freely, the auxiliary blade is respectively fixed through the left fixing screws and the right fixing screws and keeps a distance from the main blade, the soft net is a stainless steel net, the fixing clips are soft fixing clips, and the hydrogel is placed on the stainless steel net and is fixed on the stainless steel net through the soft fixing clips.
2. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the top of the box body is provided with an opening, the opening is provided with a cover plate, the size of the cover plate is matched with the size of the opening, the bottom of the box body is provided with a box bottom, four walls are arranged around the box body, and the cover plate, the box bottom and the four walls enclose a soaking cavity.
3. The graphene oxide hydrogel attached stem cell soaking device according to claim 2, wherein: the cover plate is provided with an observation structure, the observation structure is an observation window, and the observation window is made of transparent glass.
4. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the outer side of the box body is provided with a handle.
5. The graphene oxide hydrogel attached stem cell soaking device according to claim 2, wherein: the cover plate, the box body, the cell culture soaking tank and the handle are all made of 304 stainless steel.
6. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the number of the moving rods is four, and the number of the sliding rails is four, so that the sliding rails are matched with each other.
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Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005168435A (en) * | 2003-12-12 | 2005-06-30 | Fuji Photo Film Co Ltd | Apparatus for culturing cell and method for culturing cell |
KR20140098714A (en) * | 2013-01-31 | 2014-08-08 | 서울대학교산학협력단 | Scaffold including graphene fiber for cell culture and method for stem cell culture using the same |
CN105062965A (en) * | 2015-08-06 | 2015-11-18 | 深圳爱生再生医学科技有限公司 | Three-dimensional culture method for mesenchymal stem cells |
CN105238747A (en) * | 2015-09-24 | 2016-01-13 | 深圳华毓造血干细胞研究有限公司 | Method for culturing mesenchymal stem cells by artificial simulation of bone marrow microenvironment |
CN205398649U (en) * | 2016-03-08 | 2016-07-27 | 兰青 | 3D prints perfusion culture apparatus who contains cell aquogel support |
KR20170052800A (en) * | 2015-11-04 | 2017-05-15 | 중앙대학교 산학협력단 | Hydrogel for cell culturing comprising graphene oxide and medium components |
KR20170071450A (en) * | 2017-04-24 | 2017-06-23 | 서울대학교산학협력단 | 3D cell culture scaffold and co-culture method using the same |
CN111253579A (en) * | 2020-03-05 | 2020-06-09 | 大连理工大学 | Preparation method and application of glucan-hyaluronic acid hydrogel for three-dimensional cell culture |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2881434B1 (en) * | 2005-02-02 | 2007-05-11 | Coletica Sa | DEVICE FOR SUPPORTING CELL CULTURE |
US20190030211A1 (en) * | 2017-07-31 | 2019-01-31 | The Regents Of The University Of California | Hydrogel scaffold for three dimensional cell culture |
-
2020
- 2020-10-27 CN CN202011159797.1A patent/CN112300904B/en active Active
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005168435A (en) * | 2003-12-12 | 2005-06-30 | Fuji Photo Film Co Ltd | Apparatus for culturing cell and method for culturing cell |
KR20140098714A (en) * | 2013-01-31 | 2014-08-08 | 서울대학교산학협력단 | Scaffold including graphene fiber for cell culture and method for stem cell culture using the same |
CN105062965A (en) * | 2015-08-06 | 2015-11-18 | 深圳爱生再生医学科技有限公司 | Three-dimensional culture method for mesenchymal stem cells |
CN105238747A (en) * | 2015-09-24 | 2016-01-13 | 深圳华毓造血干细胞研究有限公司 | Method for culturing mesenchymal stem cells by artificial simulation of bone marrow microenvironment |
KR20170052800A (en) * | 2015-11-04 | 2017-05-15 | 중앙대학교 산학협력단 | Hydrogel for cell culturing comprising graphene oxide and medium components |
CN205398649U (en) * | 2016-03-08 | 2016-07-27 | 兰青 | 3D prints perfusion culture apparatus who contains cell aquogel support |
KR20170071450A (en) * | 2017-04-24 | 2017-06-23 | 서울대학교산학협력단 | 3D cell culture scaffold and co-culture method using the same |
CN111253579A (en) * | 2020-03-05 | 2020-06-09 | 大连理工大学 | Preparation method and application of glucan-hyaluronic acid hydrogel for three-dimensional cell culture |
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