CN112300904A - Graphene oxide hydrogel attached stem cell soaking device - Google Patents
Graphene oxide hydrogel attached stem cell soaking device Download PDFInfo
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- CN112300904A CN112300904A CN202011159797.1A CN202011159797A CN112300904A CN 112300904 A CN112300904 A CN 112300904A CN 202011159797 A CN202011159797 A CN 202011159797A CN 112300904 A CN112300904 A CN 112300904A
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- 238000002791 soaking Methods 0.000 title claims abstract description 58
- 239000000017 hydrogel Substances 0.000 title claims abstract description 44
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 30
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 229910021389 graphene Inorganic materials 0.000 title claims abstract description 27
- 238000004113 cell culture Methods 0.000 claims abstract description 21
- 210000002421 cell wall Anatomy 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 9
- 239000011148 porous material Substances 0.000 claims description 9
- 239000010963 304 stainless steel Substances 0.000 claims description 3
- 229910000589 SAE 304 stainless steel Inorganic materials 0.000 claims description 3
- 238000009826 distribution Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000012620 biological material Substances 0.000 abstract description 18
- 210000002901 mesenchymal stem cell Anatomy 0.000 abstract description 17
- 239000000463 material Substances 0.000 abstract description 15
- 229910001220 stainless steel Inorganic materials 0.000 abstract description 15
- 239000010935 stainless steel Substances 0.000 abstract description 15
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052782 aluminium Inorganic materials 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 5
- 230000008901 benefit Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 230000037314 wound repair Effects 0.000 description 2
- 206010063560 Excessive granulation tissue Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229940126672 traditional medicines Drugs 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
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Abstract
The invention discloses a graphene oxide hydrogel attached stem cell soaking device which comprises a soaking device body; the soak device body be equipped with the box, be equipped with in the box and soak the structure, timing device and moving structure, the structure of soaking soak the groove for cell culture, cell culture soaks the inslot and is equipped with soft net, moving structure for setting up at soft net carriage release lever all around, the slide rail setting soaks on the cell wall in groove at cell culture, the position that the carriage release lever set up and cell culture soak the groove and go up the slide rail position and match each other, timing device be timing controller. The invention adopts stainless steel, plastic, aluminum materials and the like to manufacture a hydrogel material carrying stem cell timed intermittent soaking device, and solves the carrying of hydrogel biological material carrying mesenchymal stem cells.
Description
Technical Field
The invention relates to the field of preparation and experiments of novel biological new materials, in particular to a graphene oxide hydrogel attached stem cell soaking device.
Background
Mesenchymal stem cells are adult stem cells with self-renewal and multidirectional differentiation potential, and become hot spots for wound repair because the mesenchymal stem cells can be differentiated into various mesenchymal tissues such as skin, blood vessels, skin appendages, bone, cartilage, fibrous tissue progenitor cells, bone marrow stroma and the like. Research shows that in the wound healing process, the induced BMSCs can be differentiated into vascular endothelium-like cells, are closely related to the formation of small blood vessels in granulation tissues, participate in wound repair, and have better prospects compared with mesenchymal stem cells treated by traditional medicines. At present, few biological materials can be used for carrying mesenchymal stem cells, the mesenchymal stem cells are limited to be applied to the treatment of chronic refractory wounds in a large range through external application, and the hydrogel has the inherent advantage as a biological material with a molecular structure similar to natural extracellular matrix (ECM) in an organism. The hydrogel not only has good hydrophilicity and is beneficial to material exchange, but also can provide good supporting and differentiation environment for cells.
However, for the current new biological materials such as hydrogels, the detailed problems that need to be noticed during the process of carrying the mesenchymal stem cells are more, for example: the method comprises the steps of soaking materials in a culture medium, fixing the materials in the soaking process, controlling the soaking process, and the like, and meanwhile, researches show that the novel biological material is more beneficial to the growth of stem cells in the culture medium containing the mesenchymal stem cells by adopting an intermittent soaking mode (namely, taking out the novel biological material after soaking the novel biological material in the culture medium for a fixed time, soaking the novel biological material after a certain time, and repeatedly operating).
Disclosure of Invention
The technical problem to be solved by the invention is that the detailed problems needing attention are more in the process of carrying mesenchymal stem cells for novel biological new materials such as hydrogel, for example: soaking time of the material in a culture medium, fixation of the material in the soaking process, time control of the soaking process and the like.
In order to solve the technical problems, the invention adopts the following technical means:
a graphene oxide hydrogel attached stem cell soaking device comprises a soaking device body; the soak device body be equipped with the box, be equipped with in the box and soak the structure, timing device and moving structure, the structure of soaking soak the groove for cell culture, cell culture soaks the inslot and is equipped with soft net, moving structure for setting up at soft net carriage release lever all around, the slide rail setting soaks on the cell wall in groove at cell culture, the position that the carriage release lever set up and cell culture soak the groove and go up the slide rail position and match each other, timing device be timing controller.
The invention adopts stainless steel, plastic, aluminum materials and the like to manufacture a hydrogel material carrying stem cell timed intermittent soaking device, and solves the carrying of hydrogel biological material carrying mesenchymal stem cells.
Preferably, the further technical scheme of the invention is as follows:
the box top be equipped with the opening, the opening part is equipped with the apron, the cover plate size and open-ended size mutually support, the bottom half is equipped with the bottom of the case, the box be equipped with four walls all around, apron, bottom of the case and four walls enclose into and soak the cavity.
The soft net on be equipped with the fixation clamp, the soft net passes through the mount to be fixed in the box, the quantity of fixation clamp is three, be equipped with the filtration pore on the soft net, the quantity of filtration pore is a plurality of, the even distribution of filtration pore is on the soft net.
The timing controller is connected with and controls the soft net to lift on the sliding rail through a power line and a data line.
The cover plate is provided with an observation structure, the observation structure is an observation window, and the observation window is made of transparent glass.
The outer side of the box body is provided with a handle.
The cover plate, the box body, the cell culture soaking tank and the handle are all made of 304 stainless steel.
The number of the moving rods is four, and the number of the sliding rails is four, so that the moving rods are matched with each other.
The soaking device body be equipped with supporting broken scissors, be by fixed screw with left main blade and right main blade connection fixed to all have a left handle and right handle respectively at the end, all fix right vice blade and left vice blade on the main blade by right fixed screw and left fixed screw respectively in the upper end of main blade, all fixed by the fixed screw, make freely rotatable, and vice blade by left fixed screw and right fixed screw respectively fixed and with main blade between keep apart from. When the tissue sample shearing device is used, the head of the scissors extends into the container to contact with sample tissues, and the handles of the scissors are opened and closed to shear the tissue samples.
The invention provides a graphene oxide hydrogel attaching stem cell timing-intermittent soaking device, which can enable biological materials such as hydrogel and the like to be more convenient and accurate in the process of attaching stem cells, obviously reduce the deviation of carrying stem cells by the hydrogel biological materials due to human factors, and enable the stem cells to grow better in the biological materials such as graphene oxide hydrogel and the like.
The invention has the following benefits and effects: the graphene oxide hydrogel attached stem cell timing-intermittent soaking device can realize full-automatic carrying of mesenchymal stem cells by a hydrogel material, realize standardized production, improve the production efficiency and standard and remarkably reduce the waste of manpower and materials.
The invention has the advantages that:
1. on the basis of carrying the mesenchymal stem cells by the original artificial control graphene oxide hydrogel biomaterial, the device realizes the full-automatic standard production of the mesenchymal stem cell hydrogel;
2. the process of carrying the mesenchymal stem cells by the hydrogel biomaterial is controllable, so that the mesenchymal stem cells on the biomaterial are distributed more uniformly;
3. the device reduces material cost and preparation time, improves work efficiency.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
Description of reference numerals: 1-cover plate; 2-a box body; 3-a timing controller; 4-soft net; 5-cell culture soaking tank; 6-observation window.
Detailed Description
The present invention will be further described with reference to the following examples.
Specific example 1:
referring to fig. 1, the graphene oxide hydrogel attached stem cell soaking device of the present invention includes a soaking device body; the soaking device body is provided with a box body 2, a soaking structure, a timing device and a moving structure are arranged in the box body 2, the soaking structure is a cell culture soaking groove 5, a soft net 4 is arranged in the cell culture soaking groove 5, the moving structure is moving rods arranged around the soft net 4, sliding rails are arranged on the groove wall of the cell culture soaking groove 5, the positions of the moving rods are matched with the positions of sliding rails on the cell culture soaking groove 5, the number of the moving rods is four, the number of the sliding rails is four, the sliding rails are matched with each other, the timing device is a timing controller 3, the top of the box body 2 is provided with an opening, a cover plate 1 is arranged at the opening, the size of the cover plate 1 is matched with the size of the opening, the bottom of the box body 2 is provided with a box bottom, four walls are arranged around the box body 2, the cover plate 1, the box bottom and the four walls are enclosed to form a soaking cavity, a fixing clamp is arranged on the soft, the quantity of fixation clamp is three, be equipped with the filtration pore on the soft net 4, the quantity of filtration pore is a plurality of, and the even distribution of filtration pore is on soft net 4, and timing controller 3 goes up and down on the slide rail through power cord and data line connection control soft net 4, is equipped with the observation structure on the apron 1, the observation structure be observation window 6, 6 transparent glass materials of observation window, the 2 outsides of box are equipped with the handle, apron 1, box 2, cell culture soak groove 5, handle are 304 stainless steel.
Specific example 2:
referring to fig. 1, the graphene oxide hydrogel attached stem cell soaking device, the graphene oxide hydrogel attached stem cell timing-intermittent soaking device, disclosed by the invention, utilizes a time controller to realize intermittent soaking of graphene oxide hydrogel in a culture medium groove containing a certain number of cells, the hydrogel is placed on a stainless steel mesh and fixed on the stainless steel mesh through a flexible fixing clamp, a fixing program is input into the time controller, the stainless steel mesh moves up and down intermittently through a sliding rail, controllable intermittent soaking of the hydrogel in the culture medium is realized, standardized operation of loading the hydrogel biomaterial on mesenchymal stem cells is realized, influence of human factors is avoided, and efficiency of loading the hydrogel on mesenchymal stem cells is greatly improved.
Specific example 3:
referring to fig. 1, the graphene oxide hydrogel attached stem cell soaking device is a special crushing shear for laboratories, and is characterized in that a left main blade and a right main blade are fixedly connected by a fixing screw, the tail ends of the left main blade and the right main blade are respectively provided with a left handle and a right handle, a right auxiliary blade and a left auxiliary blade are respectively fixed on the main blade by the right fixing screw and the left fixing screw and are fixed by the fixing screws, so that the graphene oxide hydrogel attached stem cell soaking device can freely rotate, the auxiliary blades are respectively fixed by the left fixing screw and the right fixing screw and are at a certain distance from the main blade, and the use and the shearing of the two blades are not influenced.
Specific example 4:
referring to fig. 1, the graphene oxide hydrogel attached stem cell soaking device of the present invention is composed of 5 parts, respectively: installing a stainless steel cover plate 1; installing a stainless steel box body 2; a soaking timing controller 3; a biomaterial stainless steel fixed soft net 4; stainless steel cell culture soaking tank 5.
The graphene oxide hydrogel attaching stem cell timing-intermittent soaking device is composed of a stainless steel cover and a stainless steel groove body, grooves are formed in two sides of the groove body and used for carrying, a stainless steel filter screen and a soft fixing clamp are arranged in the stainless steel groove body, a time controller can time the stainless steel mesh to move up and down along a sliding rail, and the graphene oxide hydrogel biological material on the stainless steel mesh can be completely soaked in a culture medium.
Since the above description is only a specific embodiment of the present invention, but the protection of the present invention is not limited thereto, any equivalent changes or substitutions of the technical features of the present invention which can be conceived by those skilled in the art are included in the protection scope of the present invention.
Claims (9)
1. A graphene oxide hydrogel attached stem cell soaking device comprises a soaking device body; the method is characterized in that: the soak device body be equipped with the box, be equipped with in the box and soak the structure, timing device and moving structure, the structure of soaking soak the groove for cell culture, cell culture soaks the inslot and is equipped with soft net, moving structure for setting up at soft net carriage release lever all around, the slide rail setting soaks on the cell wall in groove at cell culture, the position that the carriage release lever set up and cell culture soak the groove and go up the slide rail position and match each other, timing device be timing controller.
2. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the box top be equipped with the opening, the opening part is equipped with the apron, the cover plate size and open-ended size mutually support, the bottom half is equipped with the bottom of the case, the box be equipped with four walls all around, apron, bottom of the case and four walls enclose into and soak the cavity.
3. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the soft net on be equipped with the fixation clamp, the soft net passes through the mount to be fixed in the box, the quantity of fixation clamp is three, be equipped with the filtration pore on the soft net, the quantity of filtration pore is a plurality of, the even distribution of filtration pore is on the soft net.
4. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the timing controller is connected with and controls the soft net to lift on the sliding rail through a power line and a data line.
5. The graphene oxide hydrogel attached stem cell soaking device according to claim 2, wherein: the cover plate is provided with an observation structure, the observation structure is an observation window, and the observation window is made of transparent glass.
6. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the outer side of the box body is provided with a handle.
7. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the cover plate, the box body, the cell culture soaking tank and the handle are all made of 304 stainless steel.
8. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the number of the moving rods is four, and the number of the sliding rails is four, so that the moving rods are matched with each other.
9. The graphene oxide hydrogel attached stem cell soaking device according to claim 1, wherein: the soaking device body be equipped with supporting broken scissors, be by fixed screw with left main blade and right main blade connection fixed to all have a left handle and right handle respectively at the end, all fix right vice blade and left vice blade on the main blade by right fixed screw and left fixed screw respectively in the upper end of main blade, all fixed by the fixed screw, make freely rotatable, and vice blade by left fixed screw and right fixed screw respectively fixed and with main blade between keep apart from.
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| JP2005168435A (en) * | 2003-12-12 | 2005-06-30 | Fuji Photo Film Co Ltd | Apparatus for culturing cell and method for culturing cell |
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