CN104873289B - animal spinal fixing device - Google Patents

animal spinal fixing device Download PDF

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Publication number
CN104873289B
CN104873289B CN201510060535.2A CN201510060535A CN104873289B CN 104873289 B CN104873289 B CN 104873289B CN 201510060535 A CN201510060535 A CN 201510060535A CN 104873289 B CN104873289 B CN 104873289B
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China
Prior art keywords
fixture
plate
gear shaping
type groove
fixing device
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Expired - Fee Related
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CN201510060535.2A
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Chinese (zh)
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CN104873289A (en
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张金辉
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Individual
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D3/00Appliances for supporting or fettering animals for operative purposes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Husbandry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Surgical Instruments (AREA)

Abstract

The present invention relates to field of fixation devices, more particularly to a kind of animal spinal fixing device.The animal spinal fixing device, including the first fixture, the second fixture and accommodation space;First fixture is provided with U-type groove;The front end of the both sides of U-type groove is bent to form two the first gear shapings;Second fixture is matched somebody with somebody with U-type groove and merges and can be slided in U-type groove;Second fixture is provided with the second gear shaping coordinated with the first gear shaping;Accommodation space and two the first gear shapings coordinate, and are connected with the clamping part of the first fixture and the second fixture.By the device, brake check is carried out after animal is clear-headed, it is found that braking effect is good, can simultaneously observe a neuromere and a spinal segment for vertebral levels.And in spinal cord axons research, the spinal cord axons of animal dorsal part can be observed.

Description

Animal spinal fixing device
Technical field
The present invention relates to field of fixation devices, more particularly to a kind of animal spinal fixing device.
Background technology
1mm deep structure and function under observing animal cortex under live body even waking state under nonlinear microscopy, There is unique value in nervous physiology pathological research.In current all of research, target when ensureing observation by performing the operation Position is braked completely, is the necessary requirement of research.In the research of DRGs, isolated test rank is still trapped at present Section;In the research of spinal cord axle/dendron, the observation to animal dorsal part is only limitted under narcosis, it is impossible under realizing waking state Observation.
The content of the invention
It is an object of the invention to provide a kind of animal spinal fixing device, to solve the above problems.
In order to achieve the above object, the technical proposal of the invention is realized in this way:
A kind of animal spinal fixing device, including the first fixture, the second fixture and accommodation space;First fixture is provided with U Type groove;The front end of the both sides of U-type groove is bent to form two the first gear shapings;Second fixture is matched somebody with somebody with U-type groove and merges and can be slided in U-type groove It is dynamic;Second fixture is provided with the second gear shaping coordinated with the first gear shaping;Accommodation space and two the first gear shapings coordinate, and with first The clamping part connection of fixture and the second fixture.
Further, the animal spinal fixing device also includes plate and U-shaped plate;Plate includes baffle plate and vertical with baffle plate connects The pressure tongue for connecing;Pressure tongue is used to be inserted into two inner surfaces of the first gear shaping;Baffle plate is used to coordinate with two gear shapings of the first fixture; Thickness of the height of baffle plate more than the first gear shaping;U-shaped plate is used to be formed with baffle plate the accommodation space of all round closure.
Preferably, the second fixture also includes and two the second gear shaping sliding blocks connected vertically;Sliding block and U-type groove are with merge can Slided in U-type groove.
Preferably, fastened by screw between the first fixture and the second fixture.
Preferably, the opposite face of the first gear shaping and the second gear shaping is provided with groove.
Preferably, plate also includes Handheld Division;Handheld Division is vertical with baffle plate to be connected.
Preferably, U-shaped plate includes two intermediate plates of side plate of two side plates and connection;Two bottoms of side plate also set respectively It is equipped with base plate;The baffle plate of plate is used to cooperatively form accommodation space with two side plates.
Compared with prior art, this have the advantage that:By the hair removal of animal dorsal part and use operation Knife cuts skin, by the part musculature and tendon detachment of animal vertebra, the first gear shaping and the second gear shaping is corresponded into four Clamped in aixs cylinder position insertion musculature.Then the pressure tongue of plate is corresponded into the first gear shaping, is inserted into the interior table of the first gear shaping Face, pushes plate tail end, struts skin and musculature.After injection biogum and solidification, U-shaped plate is placed in the of the first fixture On one gear shaping, the base plate of U-shaped plate is matched somebody with somebody with the first gear shaping and merge fixation.Brake check is carried out after animal is clear-headed, braking is found Work well, can simultaneously observe a DRGs and a spinal segment for vertebral levels.And spinal cord axons are studied In, the spinal cord axons of animal dorsal part can also be observed.
Brief description of the drawings
Fig. 1 is the structural representation of the first fixture provided in an embodiment of the present invention;
Fig. 2 is the structural representation of the second fixture provided in an embodiment of the present invention;
Fig. 3 is the assembling structure schematic diagram of the first fixture provided in an embodiment of the present invention and the second fixture.
Specific embodiment
Clear, complete description is carried out to the technical scheme of various embodiments of the present invention below with reference to accompanying drawing, it is clear that retouched The embodiment stated is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, The resulting all other embodiment on the premise of creative work is not made of those of ordinary skill in the art, belongs to this The protected scope of invention.
Embodiment 1
As depicted in figs. 1 and 2, the present embodiment provides a kind of animal spinal fixing device, including the first fixture, the second fixture And accommodation space;First fixture is provided with U-type groove 102;The front end of the both sides of U-type groove 102 is bent to form two the first gear shapings 103;Second fixture is matched somebody with somebody with U-type groove 102 and merges and can be slided in U-type groove;Second fixture is provided with the coordinated with the first gear shaping Two gear shapings 202;Accommodation space coordinates with two the first gear shapings 103, and is connected with the clamping part of the first fixture and the second fixture.
Specifically, skin is cut by the hair removal of animal dorsal part and using scalpel, by the part muscle of animal vertebra Tissue and tendon detachment, clamp during the first gear shaping and the second gear shaping are corresponded into four transverse process position insertion musculatures.Then By accommodation space it was observed that spinal segment, and the spinal cord axons of animal dorsal part are observed.
Further, the animal spinal fixing device also includes plate and U-shaped plate;Plate includes baffle plate and vertical with baffle plate connects The pressure tongue for connecing;Pressure tongue is used to be inserted into two inner surfaces of the first gear shaping;Baffle plate is used to coordinate with two gear shapings of the first fixture; Thickness of the height of baffle plate more than the first gear shaping;U-shaped plate is used to be formed with baffle plate the accommodation space of all round closure.
Specifically, in operation on nerve ganglion and spinal cord axle/dendron operation, the pressure tongue of plate is corresponded into the first gear shaping, The inner surface of the first gear shaping is inserted into, plate tail end is pushed, skin and musculature is strutted.After injection biogum and solidification, will be U-shaped Plate is placed on the first gear shaping of the first fixture, the base plate of U-shaped plate is matched somebody with somebody with the first gear shaping and is merged fixation.Carried out after animal is clear-headed Brake check, it is found that braking effect is good, can simultaneously observe a neuromere and a spinal segment for vertebral levels.And In spinal cord axons research, the spinal cord axons of animal dorsal part can be observed.
Preferably, the second fixture also includes and two the second gear shaping sliding blocks 203 connected vertically;Sliding block coordinates with U-type groove And can be slided in U-type groove.Wherein sliding block can be slided in U-type groove, adjust the distance between the first gear shaping and the second gear shaping, So as to neatly loosen the first fixture and the second fixture.
Preferably, fastened by screw between the first fixture and the second fixture.When the first gear shaping and the second gear shaping are inserted To in animal muscle tissue, screw is tightened, you can the first fixture and the second fixture are fixed on animal vertebra.Although animal is clear After waking up, the first fixture and the second fixture are permanently affixed on animal.
Preferably, the opposite face of the first gear shaping and the second gear shaping is provided with groove.That is the inner surface of the first gear shaping 103 sets First groove 104 is equipped with, the outer surface of the second gear shaping 202 is provided with second groove 201.First gear shaping 103 and the second gear shaping 202 It is inserted into musculature, in order to avoid the frictional force of the first gear shaping and the second gear shaping respectively between musculature is too small, leads Cause the first gear shaping and the second gear shaping to depart from from back part of animal, groove is set in the opposite face of the first gear shaping and the second gear shaping, make the One gear shaping and the second gear shaping increase with the frictional force of musculature respectively, so as to increase firmness.
Preferably, plate also includes Handheld Division;Handheld Division is vertical with baffle plate to be connected.Handheld Division is used for handle in surgical procedure Hold, so that plate coordinates more preferably with the first fixture and the second fixture.
Preferably, U-shaped plate includes two intermediate plates of side plate of two side plates and connection;Two bottoms of side plate also set respectively It is equipped with base plate;The baffle plate of plate is used to cooperatively form accommodation space with two side plates.Specifically, two side plates of U-shaped plate and gear Plate coordinates, and forms the accommodation space of all round closure.
In the present embodiment, the concrete structure of the animal spinal fixing device is described.For clearer embodiment, this is moved Other features of thing spinal fixing device, below by example 2 to embodiment 1 in be further described.
Embodiment 2
First-selection chooses adult fluorescent transgenic mouse, and conventional animal is injected by fluorescer certainly can also realize that fluorescence is seen Examine.
According to the body weight of mouse, by the yellow Jackets of intraperitoneal injection 10mg/mL, every gram of body weight injection 0.08mg of mouse Yellow Jackets and erythromycin spongaion.
After by mouse anesthesia, mouse is transferred to carries out the shaving treatment of corresponding site away from field of operation, and uses 70% wine The row that progresses greatly is cleaned, and is then applied to corresponding shaving area with appropriate depilatory cream, uses scraper plate suitable after 3-5min is waited after smearing uniformly To strike off depilatory cream and mouse hair.Light the wiping away of physiological saline is dipped with cotton balls and remove remaining hair remover and mouse hair, so repeatedly until clear Wash clean, finally dries skin with cotton balls.
Mouse is transferred to field of operation, and covers 3 layers and cut hole gauze.
With scalpel in mouse dorsal part midsection skin, otch is 2-2.5cm.With operating forceps blunt separation skin and flesh Manadesma between meat tissue.
After finding Section four to Section six transverse process, Section four and the 5th are corresponded to curved formula eye scissors and tweezers detachment side Save the whole of transverse process, the musculature and tendon of Section six transverse process first half, exposure transverse process;With sharp tweezer at Section four and Section six It is close to the aperture of vertebra blunt separation two in the position of horizontal figure.It should be noted that the device of the present embodiment can be used for first segment to Any sections of six section transverse process, observation corresponds to second section to the 5th DRGs of section and/or spinal cord dorsal part and side successively Face axle/dendron, can select different segment to be performed the operation according to different purposes.
Bounced back after the musculature detachment of backbone side, correspond to four horizontal strokes with four teeth of the first fixture and the second fixture respectively Prominent position insertion musculature, clamps the first fixture and the second fixture and screws down.Pay special attention to:Avoid the first fixture and The gear shaping of the second fixture penetrates back of the body peritonaeum.
Mouse is transferred on trestle, the tail end of the first fixture is clamped.Two pressure tongues of plate are corresponded into the first fixture Two the first gear shapings, be inserted to its inner surface, push plate tail end, strut skin and musculature fully expose surgical field of view.
Remaining muscle etc. is further removed with curved scissors and tweezers to organize, controlled bleeding and wiped off with cotton balls to be attached on vertebra Tissue, fully exposure Section of five transverse process under DRG (i.e. DRGs).Note:Avoid damage to be covered in the hard ridge on DRG Film.
Section of five transverse process is abraded with bradawl, and vertebral plate wear down around DRG is polished slightly grand for bone plane to DRG.Use cotton Field of operation is dried in ball hemostasis.Note:Removing process needs slowly soft, it is to avoid firmly excessive worn out vertebral plate.
Appropriate Kwik-Sil biogums are injected in DRG areas, gas bubbles left has been careful not to.
Closing DRG exposed regions directly are covered with cover glass after injection, soft extruding causes that biogum enters accommodation space Other parts.
General 10-20min biogums are waited to fix.
U-shaped plate is placed on the first gear shaping top of the first fixture, and the two is bonded with a small amount of superglue.
It is coated in appropriate dental basse acrylic resin liquid acrylic acid and square groove is formed around U-shaped plate, and other spaces are filled with it, it is to avoid imaging When the extravasation of square groove reclaimed water.
After about 3-5min, dental basse acrylic resin liquid propylene acid hardening, you can cut the Handheld Division of plate afterbody.
The device edge of skin edge and the present embodiment is applied to dental basse acrylic resin liquid acrylic acid, and accommodation space is filled with it, Good closing observation ward is provided.
By the operating method of the present embodiment, it is capable of achieving the fixation of the present embodiment device, and is studied for spinal cord axons, The spinal cord axons of animal dorsal part can be observed.
The above, is only presently preferred embodiments of the present invention, and any formal limitation is not made to the present invention, is appointed What those skilled in the art, without departing from the scope of the present invention, when in the technology using the disclosure above Appearance is made a little change or is modified to the Equivalent embodiments of equivalent variations, as long as being the content without departing from technical solution of the present invention, Any simple modification, equivalent variations and the modification made to above example according to technical spirit of the invention, still fall within this In the range of inventive technique scheme.

Claims (6)

1. a kind of animal spinal fixing device, it is characterised in that including the first fixture, the second fixture, accommodation space, plate and U Template;
First fixture is provided with U-type groove;The front end of the both sides of the U-type groove is bent to form two the first gear shapings;
Second fixture is matched somebody with somebody with the U-type groove and merges and can be slided in the U-type groove;Second fixture be provided with it is described The second gear shaping that first gear shaping coordinates;
The accommodation space and two first gear shapings coordinate, and connect with the clamping part of first fixture and the second fixture It is logical;
The plate include baffle plate and with baffle plate pressure tongue connected vertically;The pressure tongue is used to be inserted into two described first The inner surface of gear shaping;The baffle plate is used to coordinate with two gear shapings of first fixture;The height of the baffle plate is more than described The thickness of the first gear shaping;
The U-shaped plate is used to be formed with the baffle plate accommodation space of all round closure.
2. animal spinal fixing device as claimed in claim 1, it is characterised in that second fixture also includes and two institutes State the second gear shaping sliding block connected vertically;
The sliding block is matched somebody with somebody with the U-type groove and merges and can be slided in the U-type groove.
3. animal spinal fixing device as claimed in claim 2, it is characterised in that first fixture and second fixture Between by screw fasten.
4. animal spinal fixing device as claimed in claim 1, it is characterised in that first gear shaping and second gear shaping Opposite face be provided with groove.
5. animal spinal fixing device as claimed in claim 1, it is characterised in that the plate also includes Handheld Division;
The Handheld Division is vertical with the baffle plate to be connected.
6. animal spinal fixing device as claimed in claim 1, it is characterised in that the U-shaped plate includes two side plates and company Connect two intermediate plates of the side plate;
The bottom of two side plates is also respectively provided with base plate;
The baffle plate of the plate is used to cooperatively form the accommodation space with two side plates.
CN201510060535.2A 2015-02-06 2015-02-06 animal spinal fixing device Expired - Fee Related CN104873289B (en)

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CN104873289B true CN104873289B (en) 2017-05-31

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108652755B (en) * 2017-03-30 2023-06-06 崔家鸣 Tendon fixing device
CN109350292B (en) * 2018-07-20 2024-06-04 中国人民解放军第四军医大学 Mouse lateral position vertebra fixed bolster
CN113413231A (en) * 2021-06-21 2021-09-21 深圳市人民医院 Fixed observation device for animal spinal nerve living body imaging and use method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102871768A (en) * 2012-09-25 2013-01-16 南京航空航天大学 Big gecko's vertebral column three-dimensional positioning device and method
CN204092265U (en) * 2014-10-23 2015-01-14 中国人民解放军总医院 A kind of animal spinal cord living imaging Orientation observation device
CN204698746U (en) * 2015-02-06 2015-10-14 张金辉 Animal spinal fixing device

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07136196A (en) * 1993-11-18 1995-05-30 Yoshihiro Kishigami Atlas/epistropheus fixing device for veterinarian
US7337005B2 (en) * 2004-09-08 2008-02-26 Spinal Modulations, Inc. Methods for stimulating a nerve root ganglion

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102871768A (en) * 2012-09-25 2013-01-16 南京航空航天大学 Big gecko's vertebral column three-dimensional positioning device and method
CN204092265U (en) * 2014-10-23 2015-01-14 中国人民解放军总医院 A kind of animal spinal cord living imaging Orientation observation device
CN204698746U (en) * 2015-02-06 2015-10-14 张金辉 Animal spinal fixing device

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