CN217908079U - Animal anaesthesia device suitable for two-photon microscope - Google Patents

Abstract

The utility model provides an animal anaesthesia device suitable for a two-photon microscope, which comprises a square box, a cylindrical pipe, an electric blanket and a rolling shaft, wherein the square box is enclosed by a bottom plate and four side walls to form a square box body; the rolling shaft can be movably arranged at the bottom in the square box; the bottom plate is divided into two layers, and the electric blanket is arranged between the two layers of bottom plates. The utility model provides an animal anaesthesia device can adopt the building blocks concatenation to form, but adjusting device's size, the roll axis of design can adjust experimental animals's the bulge position in a flexible way, can pinpoint the position of mouse intrathecal injection to can be better nimble, fix the position spinal cord that the mouse injected steadily, it is stable for a long time to make it maintain under certain specific high angle, more match with two-photon microscope, the accurate focus of being convenient for adjustment and long-time maintenance.

Description

Animal anaesthesia device suitable for two-photon microscope

Technical Field

The utility model belongs to the technical field of animal experiment equipment, concretely relates to animal anaesthesia device suitable for two-photon microscope.

Background

The gas anesthesia device in the prior art consists of a bottom plate, a cylindrical pipe, a medical adhesive tape and a rolling shaft and is used for performing operation operations such as intrathecal injection of spinal cords of rats and rats. The existing intrathecal injection device for rats and mice mainly comprises a base plate and a gas injection device, and can be used for intrathecal injection after gas anesthesia of rats and mice. Specifically, a tube for gas anesthesia is arranged in a gas anesthesia device, the gas anesthesia device is fixed by a medical adhesive tape and the like, and when the intrathecal injection is performed on a mouse, the rolling shaft is movably arranged at the position of the spine bulge under the mouse body. However, due to the difference of the individual body shapes of the experimental mouse animals, the head of the experimental mouse animal is difficult to adjust the depth extending into the anesthetic tube when the experimental mouse animal is anesthetized; when the intrathecal injection operation of the mouse is carried out, the roller under the experimental animal of the mouse cannot be fixed, so the convenient adjustment or the long-term fixation is difficult, and the phenomena of defocusing, coke running and difficult focusing can cause the difficult fixation during the injection when the intrathecal injection operation of the mouse is butted with a precision detection instrument such as a two-photon microscope platform and the like; if the ambient temperature is too low before anesthesia, the experimental mouse animal is anxious and unstable before anesthesia, the physiological and biochemical states are changed in a stress manner during anesthesia, the anesthesia time is prolonged, and long-time detection with the time axis as a variable is difficult to realize.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides an animal anaesthesia device suitable for two-photon microscope.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

an animal anesthesia device suitable for a two-photon microscope comprises a square box, a cylindrical pipe, an electric blanket and a rolling shaft, wherein the square box is surrounded by a bottom plate and four side walls to form a square box body; the rolling shaft can be movably arranged at the bottom in the square box; the electric blanket is arranged between the bottom plate and the upper plate.

According to the animal anesthesia apparatus, preferably, the rolling shafts are movably clamped on the side walls of the two opposite sides of the square box, and the rolling shafts are arranged close to the bottom of the square box; the outer ends of one side or two sides of the rolling shaft are provided with bulges, and correspondingly, two side walls connected with the rolling shaft are provided with grooves clamped with the bulges.

Preferably, the bottom plate and the four side walls are formed by splicing building blocks, and the building blocks are provided with protrusions and grooves which are matched with the protrusions for insertion.

In the animal anesthesia apparatus, the base plate is preferably square with a long side of 20-25 cm and a width of 15-20 cm, and the building blocks on the side wall are formed by splicing building blocks matched with the base plate in length.

In the animal anesthesia apparatus, preferably, the upper plate is provided with upper side strips respectively near two opposite side walls, the upper surface of the upper side strip is provided with a plurality of spaced protrusions, the lower surface of the upper side strip is provided with grooves, and the upper plate is provided with protrusions for clamping and fixing at positions corresponding to the grooves on the lower surface of the upper side strip; two ends of the rolling shaft can be respectively clamped between two adjacent bulges of the side strips at the upper layer side, and the rolling shaft is fixed.

According to the animal anesthesia device, preferably, the bottom side edge strip is clamped and fixed with the upper plate and the lower plate through the protrusions and the grooves.

The beneficial effects of the utility model reside in that:

the utility model provides an animal anaesthesia device, can adopt building blocks concatenation to form, can be as required, the size of adjusting device at any time, the rolling axis of design can adjust experimental animals spinal cord bulge position in a flexible way, maintain the stable angle and the height in appointed spinal cord position, compare in the rolling axis design of prior art, can not only pinpoint the position of mouse intrathecal injection, and can better nimble, fix the position spinal cord that the mouse injected well in a flexible way, steadily, make it maintain stable for a long time under certain specific altitude angle, more match with the two-photon microscope, the teaching and long-time maintenance of accurate focus of being convenient for; compared with the existing anesthesia device, the utility model is also added with an electric blanket, which relieves the tension and uneasiness of the experimental animal of the mouse before anesthesia and stabilizes the physiological and biochemical indexes of the experimental animal in anesthesia; the utility model discloses replace gas anesthesia machine with 50 mL's cylindrical pipe and anaesthetize the mouse, greatly made things convenient for experimenter's operation.

Drawings

Fig. 1 is a top view of the animal anesthesia apparatus of the present invention suitable for the two-photon microscope.

Fig. 2 is a schematic diagram of the present invention showing a partial structure in example 2.

[ description of reference ]

1: a square box;

2: a cylindrical tube;

3: a rolling shaft;

4: a side wall;

5: a circular hole;

6: a medical adhesive plaster;

7: a protrusion;

8: an upper plate;

9: an upper layer side edge strip;

10: a bottom layer plate;

11: a bottom layer side edge strip;

12: an electric blanket.

Detailed Description

The following examples are intended to further illustrate the invention but should not be construed as limiting the invention. Without departing from the spirit and essential premise of the utility model, the decoration or replacement of the utility model all belongs to the category of the utility model.

Unless otherwise indicated, the technical means used in the examples are conventional means well known to those skilled in the art, and unless otherwise specified, the reagents used in the methods are analytically pure or above.

Example 1

The utility model provides an animal anaesthesia device suitable for two-photon microscope, as shown in figure 1, it includes square box 1, cylindrical pipe 2, electric blanket and roll axis 3, square box 1 encloses into a side box by bottom plate and four lateral walls 4, one side of square box infrabasal plate is located to cylindrical pipe 2, the well lower part of cylindrical pipe 2 is fixed on the bottom plate with medical adhesive tape 5, the side of cylindrical pipe 2 is equipped with circular port 6, circular port 6 is located the inside of square box 1, be used for inserting and be equipped with the isoflurane liquid, can anaesthetize the animal of placing in square box 1, the volume of cylindrical pipe can be set to 50mL. The bottom plate of the square box 1 is divided into a bottom plate and an upper plate, bottom side strips are arranged between the bottom plate and the upper plate at intervals, and two bottom side strips are arranged and are arranged oppositely; an electric blanket is arranged between the bottom plate and the upper plate, and is used for maintaining the body temperature of an animal (such as a mouse or a rat) while performing intrathecal injection on the animal. The bottom in the square box is located to 3 mobile nature cards of roll axis, and 3 mobile nature cards of roll axis are on the relative both sides lateral wall 4 of square box, and the roll axis sets up near the bottom of square box, and the effect of roll axis is protruding animal like the backbone part of big mouse, conveniently operates the spinal cord of big mouse and small mouse. Specifically, the outer ends of one side or two sides of the rolling shaft 3 are provided with protrusions 7, and correspondingly, two side walls connected with the rolling shaft are provided with grooves for clamping the protrusions. The protrusions at one end or two ends of the rolling shaft 4 are clamped into the grooves of the side walls, so that the position of the rolling shaft can be manually adjusted.

The bottom plate and the four side walls 4 are formed by splicing building blocks, the building blocks of the bottom plate and the side walls are respectively provided with a protrusion and a groove which is matched with the protrusion for splicing, the specification of the bottom plate can be set to be a square with a long edge of 20cm-25cm and a width of 15cm-20cm, and the building blocks of the side walls are matched with the length of the bottom plate. The two layers of bottom plates, namely the bottom plate and the upper plate, are all building blocks and are used for being spliced with the side wall. The structures of the sidewall building blocks and the bottom plate building blocks and the upper plate building blocks are all provided with bulges on the upper surfaces, and grooves matched with the bulges and used for splicing are arranged on the lower surfaces.

Example 2

The difference of the embodiment lies in the arrangement of the rolling shaft 3 on the basis of the embodiment 1, as shown in fig. 2, the rolling shaft 3 in the embodiment is provided with an upper layer side edge strip 9 through the clamping connection of two opposite side edges on an upper layer plate 8, a plurality of spaced protrusions are arranged on the upper surface of the upper layer side edge strip 9, a groove is arranged below the upper layer side edge strip 9, a protrusion clamped with the groove is correspondingly arranged on the upper layer plate, and the groove and the protrusion fix the upper layer plate and the upper layer side edge strip together. The adjacent raised interval position is used for joint roll axle 3 on upper strata side strake 9, and two upper strata side strakes set up relatively, can be fixed roll axle 4 when using, can change the position of joint as required again. The bottom plate 10 and the upper plate 8 are spaced by the bottom side strips 11 which are oppositely arranged, and an electric blanket 12 is arranged between the bottom plate 10 and the upper plate 8. The bottom layer side edge strip 11 is the same as the upper layer side edge strip 9, a plurality of bulges are arranged on the upper surface, a plurality of grooves corresponding to the bulges are arranged on the lower surface of the bottom layer side edge strip, and the bulges clamped with the lower surface of the bottom layer side edge strip 11 are arranged on the bottom layer plate 10. The utility model discloses a set up protruding and recess joint setting of accessible, simple to operate.

The utility model discloses an anaesthesia device whole volume is less, when using, opens the power of electric blanket, puts into the anaesthetic in cylindrical pipe 2, places big mouse in the square box, anaesthetizes. The position of the rolling shaft 3 can be adjusted as required, the whole device can be placed between two-photon microscope objective lenses, and the structures of the designated sections of the spinal cords of the big mouse and the small mouse can be clearly observed by forming a vertical angle between the designated sections of the spinal cords of the big mouse and the small mouse and the objective lenses, so that the visual field is clearer. In the utility model, 1) the heating pad is arranged in the middle of the bottom plates of the upper layer and the lower layer, so that the big mouse and the small mouse are more stable in state, and the dynamic change of the big mouse and the small mouse can be conveniently observed for a long time; 2) The simple anesthesia device is fixed on the bottom plate, so that the space occupation of a large anesthesia machine is saved. 3) The rolling shaft and the groove on the side wall are used together, so that the designated sections (front and back of the rolling shaft) of the spinal cords of the big and small mice can form a vertical angle with the ocular lens of the microscope for a long time, and within the focal length regulation range of the microscope, the calcium ion flow or fluorescence co-location signal of the specific sections of the spinal cords can be accurately monitored for a long time.

The four walls of the square box are formed by splicing detachable building blocks, and the size of the square box can be conveniently set according to the size of an animal.

The novel experiment model has the advantages that the bottom plate and the side wall are fixed by building blocks, the bottom plate and the protruding part for the rolling shaft can be adjusted at will due to the characteristic, the rolling shaft can be fixed at any position of the long shaft, and the individual difference of mouse experimental animals can be better adapted; secondly, an electric blanket is added between the two layers of bottom plates, so that the body temperature of the mouse can be maintained when the mouse experimental animal performs intrathecal injection, anxiety of the mouse experimental animal in the early stage of anesthesia is relieved, the anesthesia time of the mouse is shortened, the stability of the detection environment and the consistency of the body position and the state of the experimental animal are maintained, the external environment interference is eliminated, the adaptability of the mouse experimental animal to detection equipment is improved, and the reliability, the repeatability and the sensitivity of detection are improved; moreover, the anesthesia device replaces a gas anesthesia machine with a 10ml syringe, thereby greatly facilitating the operation of experimenters and simplifying the gas anesthesia process.

Claims (6)

1. An animal anesthesia device suitable for a two-photon microscope is characterized by comprising a square box, a cylindrical pipe, an electric blanket and a rolling shaft, wherein the square box is surrounded by a bottom plate and four side walls to form a square box body; the rolling shaft can be movably arranged at the bottom close to the square box; the electric blanket is arranged between the bottom plate and the upper plate.

2. The animal anesthesia apparatus of claim 1, wherein the rolling axis is movably engaged with the side walls of the square box at opposite sides thereof, the rolling axis being disposed near the bottom of the square box; the outer ends of one side or two sides of the rolling shaft are provided with bulges, and the two corresponding side walls connected with the rolling shaft are provided with slots clamped with the bulges.

3. The animal anesthesia apparatus of claim 1, wherein the bottom plate and the four sidewalls are formed by splicing building blocks, each building block having a protrusion and a groove matching with the protrusion for insertion.

4. The animal anesthesia apparatus of claim 1, wherein the base plate is formed in a square shape having a long side of 20cm to 25cm and a width of 15cm to 20cm, and the blocks of the sidewall are formed by splicing blocks whose lengths are matched with those of the base plate.

5. The animal anesthesia apparatus of claim 1, wherein the upper plate is provided with upper side strips adjacent to the opposite side walls, the upper surface of the upper side strip is provided with a plurality of spaced protrusions, the lower surface of the upper side strip is provided with grooves, and the upper plate is provided with protrusions for engaging and fixing at positions corresponding to the grooves on the lower surface of the upper side strip; the two ends of the rolling shaft can be respectively clamped between two adjacent bulges of the side strips at the two upper layers, and the rolling shaft is fixed.

6. The animal anesthesia apparatus of claim 1, wherein the bottom side tabs are snap fit into the upper and lower plates with protrusions and recesses provided therebetween.

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