CN217438201U

CN217438201U - A orifice plate for cell mar experiment and supplementary device of shooing thereof

Info

Publication number: CN217438201U
Application number: CN202220718055.6U
Authority: CN
Inventors: 杨柳
Original assignee: Tianjin Kangting Biological Engineering Group Co Ltd
Current assignee: Tianjin Kangting Biological Engineering Group Co Ltd
Priority date: 2022-03-30
Filing date: 2022-03-30
Publication date: 2022-09-16
Anticipated expiration: 2032-03-30

Abstract

The utility model relates to an orifice plate and supplementary device of shooing for cell mar experiment, including the culture plate and with the cultivation lid of culture plate cooperation installation, the culture plate includes the orifice plate body, be equipped with the diaphragm orifice on the orifice plate body, orifice plate body four sides all are equipped with the support backplate admittedly, the support backplate lower surface is equipped with the scale mark along orifice plate body edge, and the equal symmetrical system in orifice plate body upper surface both sides that corresponds the vertical central axis position of diaphragm orifice has the mar guide slot, and the cultivation that corresponds the vertical central axis position of diaphragm orifice is covered and has still been seted up the mar passageway, and this passageway is used for the rifle point to pass and carry out the mar experiment to the downthehole cell of diaphragm orifice. The utility model discloses novel structure, easy operation, the cooperation of mar guide slot and mar passageway is used, can improve cell mar accuracy greatly, and owing to the setting of the scale mark on the support backplate and mark line, to the reading of experimental data after being convenient for shoot, simplifies experimental operation, improves experimental efficiency.

Description

A orifice plate for cell mar experiment and supplementary device of shooing thereof

Technical Field

The utility model belongs to biological assay research field relates to cell molecule experimental apparatus, especially a orifice plate and supplementary device of shooing for cell mar experiment.

Background

The cell scratching test (round Healing) is an in vitro test method for researching cell migration/tumor invasion, the method simulates the migration process of cells in the Healing process in vivo, is a simple and easy method for detecting cell movement, is simple to operate, is economical and practical, and is one of the earliest developed methods for researching the in vitro migration of directional cells.

The principle of the method is that when the cells grow to be fused into a single layer state, a blank area called as a scratch is artificially made on the fused single-layer cells, the cells at the edge of the scratch gradually enter the blank area to heal the scratch, the process of in vivo cell migration is simulated to a certain extent, and the method is the simplest method in an in vitro experiment for researching the cell migration. The basic steps include creating a "Wound" in the cell monolayer, observing the Wound at the beginning and by capturing images periodically during cell migration, and comparing the images to determine the rate of cell migration, as it is similar to the in vitro Wound Healing process, also known as the Wound Healing Assay (Wound-Healing Assay), widely used to allow observation of the effects of exogenous factors such as drugs, genes, etc. on cell migration and repair.

In the conventional scratch experiment method in the current scientific research experiment, a marker pen is used for drawing 5 uniform and parallel transverse lines on the back of a 6-hole plate, cells are paved in the hole plate, after the cells grow uniformly, a scratch wound is made by a gun head or a toothpick, and after the cells migrate, the cells are observed and photographed under a microscope. By using the traditional method, although the scratch is simple to operate, the scratch cannot be kept uniform due to the fact that the strength of the scratch is different, the distance between the 5 lines is difficult to ensure to be the same, observation is inconvenient to conduct during photographing, an experimental result is affected, and sundries are easily brought into each hole when each hole is frequently photographed, so that cell pollution and the like are caused.

No patent documents relevant to the present application were found by search.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art's weak point, provide a orifice plate for cell mar experiment and supplementary device of shooing thereof to reach the purpose of simple operation more in phase, solved the inaccurate problem of current cell mar orifice plate mark scale, can assist simultaneously and shoot under the microscope, need not adjust the microscope repeatedly and observe the position of ruling, simplify the experiment operation, improve experimental efficiency.

The utility model provides a its technical problem take following technical scheme to realize:

the utility model provides an orifice plate and supplementary device of shooing for cell mar experiment, including cultivate the board and with the cultivation lid of cultivateing the board cooperation installation, it includes the orifice plate body to cultivate the board, surface arrangement system has the pore plate on the orifice plate body, orifice plate body four sides all is equipped with the support backplate admittedly, the support backplate lower surface is equipped with the scale mark along orifice plate body edge, and the equal symmetrical system in orifice plate body upper surface both sides that corresponds the vertical center axis position of pore plate has the mar guide slot for the accurate central point who confirms the cell mar of ruler is put to accurate the inserting, and the cultivation that corresponds the vertical center axis position of pore plate is covered and has still seted up the mar passageway, and this passageway is used for the rifle point to pass and carry out the mar experiment to the downthehole cell of plate.

Moreover, the culture plate and the culture cover are made of transparent acrylic materials.

And the lower surface of the orifice plate body corresponding to the transverse central axis and the longitudinal central axis of the orifice plate is respectively provided with a transverse marking line and a longitudinal marking line, and the transverse marking line and the longitudinal marking line are intersected at the central point of each orifice plate hole to determine the central point.

And, support columns are installed at four corners of the lower surface of the support guard plate.

The utility model has the advantages that:

the utility model is suitable for a cell mar experiment, a cell migration is used for studying, moreover, the steam generator is simple in structure, and reasonable design, and is simple and convenient to operate, the device can be operated in arranging experiment operation panel in, also can be used to shoot under the microscope, aseptic technique has been realized, the device sets up the position of central line clearly visible at cell culture plate lower surface, in the design culture plate, cell mar apron has independently been designed, it is not straight to the scale mark with the ruler among the traditional approach to have solved, the inhomogeneous problem in position, during the time use at orifice plate body edge design mar guide slot, can be more convenient and fast's location mar position, and the experiment efficiency is improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic bottom view of the present invention.

Detailed Description

The present invention will be described in more detail with reference to the following embodiments, which are provided by way of illustration and not limitation, and the scope of the present invention should not be limited thereby.

A pore plate for cell scratch experiment and an auxiliary photographing device thereof comprise a culture plate 2 and a culture cover 1 arranged on the culture plate, the culture plate comprises a pore plate body 2-1, the upper surface of the pore plate body is provided with pore plates 2-2 in an arrangement way, the four sides of the orifice plate body are fixedly provided with support guard plates 2-4 to increase the stability of the culture plate, the lower surface of the supporting guard plate is provided with scale marks 2-9 along the edge of the orifice plate body, which is convenient for marking during experiments, scratch guide grooves 2-3 are symmetrically arranged on both sides of the upper surface of the orifice plate body corresponding to the longitudinal central axis of the orifice plate, the cell scratching test device is used for accurately inserting a ruler to accurately determine the central position of cell scratches, and a scratch channel 1-1 is also arranged on a culture cover corresponding to the longitudinal central axis position of a plate hole and used for enabling a gun tip to penetrate through and carrying out a scratch test on cells in the plate hole;

the utility model discloses a culture plate is made for transparent ya keli material, the utility model discloses a for better carry out mar and the shooting to the cell of mar experiment, the lower surface of the orifice plate body that corresponds the horizontal center pin of diaphragm orifice and vertical center pin position sets up horizontal mark line 2-8 and vertical mark line 2-7 respectively, horizontal mark line and vertical mark line all intersect at every diaphragm orifice central point, confirm central point 2-6, regard this central point as the observation point of shooing fixed, the unsettled problem of position when observing around solving;

the utility model discloses in the implementation, support backplate under surface mounting has support column 2-5 for the culture plate lower surface does not contact with the experiment mesa, avoids in the use, and because sprayed alcohol etc. and influential to the mark line of bottom, thereby influence the mar and shoot the record.

The utility model has novel structure and convenient use, compared with the traditional orifice plate, the bottom of the orifice plate does not need to be manually marked before the experiment, and the scratch is more accurate due to the arrangement of the scratch guide groove; still having cooperated one at this device and having cultivateed the lid, covering in the cultivation and being provided with the mar passageway, this passageway is applicable to 10 mu L rifle point and 200 mu L rifle point and passes through, can ensure the degree of accuracy of mar, during the use, need not open repeatedly and cultivate the lid, avoids polluting the cell of cultivateing in the culture plate, ensures that temperature and experiment temperature in the culture plate are unanimous, reduces the culture plate and takes out because of the vapor that the difference in temperature produced from the incubator, guarantees the result of shooing.

The utility model discloses specific use:

before the device is used for carrying out a scratch experiment, other parallel lines of the scratch are determined at the bottom of the culture plate through the scale lines on the lower surface of the supporting protection plate and the transverse marking lines, and each plate hole is ensured to penetrate through 5 transverse parallel lines; when the cell mar, plant the required cell of experiment in the pore plate of culture plate, then cover the cultivation lid, insert the ruler scratch passageway and clamp with the scratch guide slot on the orifice plate body, ensure that the ruler is overlooking projection and vertical mark line heavy line, guaranteed that ruler and horizontal mark line and other parallel lines are perpendicular, then the manual work inserts the rifle head with the pipettor, puts into the scratch passageway with the rifle head, carries out the mar to the cell.

Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.

Claims

1. The utility model provides a orifice plate and supplementary device of shooing for cell mar experiment, including cultivate the board and with the cultivation lid of cultivateing the board cooperation installation, it includes the orifice plate body to cultivate the board, orifice plate body upper surface arrangement system has the plate hole, its characterized in that: the utility model discloses a cell scratch test instrument, including orifice plate body, ruler, support backplate, the equal system of orifice plate body upper surface both sides that correspond the longitudinal center axis position of diaphragm orifice, the equal solid support backplate that is equipped with of orifice plate body four sides, support the backplate lower surface and be equipped with the scale mark along orifice plate body edge, the equal symmetry system in orifice plate body upper surface both sides that corresponds the longitudinal center axis position of diaphragm orifice has the mar guide slot for accurate central point that confirms the cell mar of inserting the ruler puts, and the cultivation that corresponds the longitudinal center axis position of diaphragm orifice is covered and has still seted up the mar passageway, and this passageway is used for the rifle point to pass and carries out the mar experiment to the downthehole cell of diaphragm orifice.

2. The well plate and the auxiliary photographing device thereof for the cell scratch experiment according to claim 1, wherein: the culture plate and the culture cover are made of transparent acrylic materials.

3. The well plate and the auxiliary photographing device thereof for the cell scratch experiment according to claim 1, wherein: and transverse marking lines and longitudinal marking lines are respectively arranged on the lower surface of the orifice plate body corresponding to the transverse central axis and the longitudinal central axis of the orifice plate, and the transverse marking lines and the longitudinal marking lines are intersected at the central point of each orifice plate hole to determine the central point.

4. The well plate and the auxiliary photographing device thereof for the cell scratch experiment according to claim 1, wherein: support columns are arranged at four corners of the lower surface of the support guard plate.