CN206616225U

CN206616225U - A kind of cell scoring devices

Info

Publication number: CN206616225U
Application number: CN201720369145.8U
Authority: CN
Inventors: 殷刚; 李医民; 张逸敏; 张辉
Original assignee: Central South University
Current assignee: Central South University
Priority date: 2017-04-10
Filing date: 2017-04-10
Publication date: 2017-11-07
Anticipated expiration: 2027-04-10

Abstract

The utility model discloses a kind of cell scoring devices, including culture plate and cut pipette tips, the culture plate top surface offers some culture holes, the culture plate top surface is provided with cut plate lid, bottom surface is provided with cut location-plate, the cut location-plate is provided with the graduated scale of position observation cell migration distance, the cut plate covers each corresponding culture hole and offers some raised cut guiding ribs, cut gap is equipped with every cut guiding rib, the cut pipette tips include barral of a gun and cut pin, the cut pipette tips are socketed with inverted U interface card by subapical part, the cut guiding rib is inserted in the inverted U interface card and is oriented to during cut, the cut pipette tips upper end is provided with the jack connected for liquid-transfering gun.The characteristics of the utility model has with low cost, simple to operate, universal strong, U-shaped interface card can effectively control cut dynamics with the design that cut guiding rib agrees with, it is ensured that cut shape, the uniformity of size.

Description

A kind of cell scoring devices

Technical field

The utility model belongs to technical field of medical instruments, more particularly to a kind of cell scoring devices.

Background technology

Cell scratch experiment is a kind of simple, practical, classical cell experiment method, the migration for studying cell in vitro Acted on invasion and attack.At present also it is untapped go out complete effective scoring devices, the existing Main process steps of cell scratch experiment are： 1. experiment the previous day, by cell kind plate in six orifice plates, compares by using ruler, is rule in six orifice plates with liquid-transfering gun pipette tips.② Covered and made marks in six orifice plates with marker, taken pictures taken pictures with reference to the same site that is marked at of marker every time.By Often cause that the line marked is uneven in its limited experimental tool, dynamics is uneven to cause scratch width to differ, marker Mark is difficult to find identical site every time.So that existing in experimental result afterwards and data analysis certain inclined Difference, in order to improve this problem, the cell scoring devices for developing complete set are significant.

Utility model content

The purpose of this utility model is to provide a kind of cut accurate positioning, convenient and swift, economical and practical cell cut Device.

In order to solve the above technical problems, cell scoring devices provided by the utility model, including culture plate and cut rifle Head, the culture plate top surface offers some culture holes, and the culture plate top surface is provided with cut plate lid, and bottom surface is fixed provided with cut Position plate, the cut location-plate is provided with the graduated scale of position observation cell migration distance, and it is each that the cut plate covers correspondence Individual culture hole offers some raised cut guiding ribs, and cut gap, described stroke are equipped with every cut guiding rib Trace pipette tips are socketed with inverted U interface card by subapical part, and the cut guiding rib inserts the inverted U interface card during cut Interior to be oriented to, the cut pipette tips upper end is provided with the jack connected for liquid-transfering gun.

Further, the cut pipette tips are common tip heads.

Further, the cut pipette tips tip diameter is 0.5mm or 1mm or 2mm.

Further, the cut plate lid is by being arranged at the catch spiral-lock at its edge on culture plate.

Further, the culture plate is six well culture plates.

The utility model is drawn to be agreed with by the cut guiding rib and inverted U interface card perfection of cut plate lid, can effectively be controlled Cut dynamics, it is ensured that the width and shape of cut；Cell cut pipette tips of the present utility model directly can be placed on liquid-transfering gun, behaviour Facilitate, reduce pollution；Cut pipette tips can also be replaced with common tip heads, economical and practical；Tradition is replaced with cut location-plate Marker pens mark reduce the error in manual operation, cut accurate positioning, repeatability are high.

Brief description of the drawings

Fig. 1 is the schematic top plan view of the utility model cut plate lid；

Fig. 2 is the use schematic diagram of the utility model cut gun apparatus；

Fig. 3 is the schematic diagram of the utility model cut location-plate；

Wherein：1- cut plate lids；2- cut pipette tips；3- cut location-plates；4- cuts gap；5- cut guiding ribs；6- rifles Bar；7- inverted U interface cards；8- tips；9- culture plates.

Embodiment

Below in conjunction with the drawings and specific embodiments, the utility model is further described.

As Figure 1-3, cell scoring devices provided by the utility model, including culture plate 9 and cut pipette tips 2, institute It is six usually conventional well culture plates to state culture plate 9, and the top surface of culture plate 9 is provided with cut plate lid 1, and bottom surface is fixed provided with cut Position plate 3, catch spiral-lock of the cut plate lid 1 by being arranged at its edge is set on culture plate 9 on the cut location-plate 3 There is on the graduated scale of position observation cell migration distance, the cut plate lid 1 each culture hole of correspondence offer some convex The cut guiding rib 5 risen, is equipped with cut gap 4 on every cut guiding rib 5, and the cut pipette tips 2 lean on subapical portion Divide and be socketed with inverted U interface card, the cut guiding rib is inserted in the inverted U interface card 7 and is oriented to during cut, the cut rifle Head upper end is provided with the jack connected for liquid-transfering gun, specifically, the cut pipette tips 2 are by barral of a gun 6 and connection and stroke of barral of a gun lower end Trace pin 8 is constituted, and the tip diameter of cut pin 8 can select 0.5mm or 1mm or 2mm, institute according to different scratch width requirements State on the top that jack is arranged at the barral of a gun 6, the suit of inverted U interface card 7 and the barral of a gun 6, during cut, the cut is oriented to It is used to cut in the insertion inverted U of muscle 5 interface card 7 be oriented to.

Described cut location-plate, cut plate lid, culture plate length and width size it is identical, be 12.7cm × 8.5cm, cut positioning plate thickness is 2.5mm, and is transparent panel, and cut location-plate is marked with 6 mark lines.

It is described as follows using the experimentation of above-mentioned super practical cell cut rifle experimental rig：

Cut plate lid 1 is buckled on six well culture plates 9, and cut pipette tips 2 are arranged on liquid-transfering gun, cut pipette tips 2 are inserted Enter in cut gap 4, in the insertion inverted U of cut guiding rib 5 interface card 7, cut pipette tips 2 are slided to separately from the one end of cut gap 4 One end, cut location-plate 3 is placed on the lower section of six well culture plate 9, the graduated scale that microscope is focused on to cut location-plate 3, selection Cut is taken pictures site, writes down its corresponding coordinate；Focus and taken pictures in cell plane, put at regular intervals in same coordinate Taken pictures in site.

Embodiment 2

The present embodiment and the difference of embodiment 1 are that the cut pipette tips 2 are common tip heads, remaining be the same as Example 1.

Claims

1. a kind of cell scoring devices, including culture plate (9) and cut pipette tips (2), culture plate (9) top surface offer some Culture hole, it is characterised in that：Culture plate (9) top surface is provided with cut plate lid (1), and bottom surface is provided with cut location-plate (3), institute State and correspond to each on the graduated scale that cut location-plate (3) is provided with position observation cell migration distance, the cut plate lid (1) Culture hole offers some raised cut guiding ribs (5), and cut gap (4) is equipped with every cut guiding rib (5), The cut pipette tips (2) are socketed with inverted U interface card (7) by subapical part, cut guiding rib (5) insertion during cut It is oriented in the inverted U interface card (7), cut pipette tips (2) upper end is provided with the jack connected for liquid-transfering gun.

2. cell scoring devices according to claim 1, it is characterised in that：The cut pipette tips (2) are common tip heads.

3. cell scoring devices according to claim 1, it is characterised in that：Cut pipette tips (2) tip diameter is 0.5mm or 1mm or 2mm.

4. cell scoring devices according to claim 1, it is characterised in that：The cut plate lid (1) is by being arranged at it The catch spiral-lock at edge is on culture plate (9).

5. the cell scoring devices according to claim any one of 1-4, it is characterised in that：The culture plate (9) is six holes Culture plate.