CN210163438U

CN210163438U - Cell scratching device

Info

Publication number: CN210163438U
Application number: CN201920729982.6U
Authority: CN
Inventors: 汪晓强; 田婕; 俞卫锋
Original assignee: Renji Hospital Shanghai Jiaotong University School of Medicine
Current assignee: Renji Hospital Shanghai Jiaotong University School of Medicine
Priority date: 2019-05-20
Filing date: 2019-05-20
Publication date: 2020-03-20
Anticipated expiration: 2029-05-20

Abstract

A cell scratching device belongs to the field of biotechnology experimental equipment. The sheet bending piece is a rectangular sheet bending piece with two bent short edges and consists of a buckle, a central hollow groove, a horizontal panel and a graduated scale structure. Through special buckle and central dead slot, help the liquid-transfering gun rifle head to stabilize in the cell mar experiment and scratch without lateral displacement, obtain straight aequilate mar line, accomplish the cell mar experiment fast effectively. This cell mar ware simple structure, economical and practical can use repeatedly, and convenient environmental protection can greatly improve cell mar experiment success rate.

Description

Cell scratching device

Technical Field

The utility model relates to a cell mar ware belongs to biotechnology experimental apparatus field.

Background

The cell scratch experiment is a commonly used experiment for detecting cell migration capacity in scientific research work, and is particularly widely applied to research in the field of tumors. The experimental principle is as follows: the migration ability of cells was analyzed by artificially creating a rectangular area without cells on the bottom of a culture dish (or plate) full of cells by experimental tools, and then observing the speed at which the cells again full of the rectangular empty area. The currently common methods are: a ruler is placed on the edge wall of a cell culture dish (or culture plate) with the bottom full of cells, the ruler is placed in the air relative to the bottom of the cell culture dish (or culture plate), and then a line is drawn on the bottom plate of the cell culture plate along the edge of the ruler by using a pipette tip. When the method is used, the cell culture dish and the cell culture plate have certain depths, the ruler is placed in a suspended mode, the hand for holding the pipette head is in a suspended state during scribing, and the pipette head is difficult to ensure to be perpendicular to the culture plate all the time during scribing by depending on the edge of the straight ruler during scribing. When the line is scribed, the left and right and even the front and back shift are easy to generate, which all leads to the difficulty of ensuring the straight line and the consistent width of the line. The oblique scribing line and the scribing line with inconsistent width seriously affect the analysis of experimental results, and the difficulty in shooting beautiful and high-quality experimental pictures is one of the main reasons for experimental failure. Therefore, an auxiliary experimental device capable of helping to complete a straight cell scribing line with the same width is needed.

Disclosure of Invention

The utility model aims at providing a cell mar ware for solving the technical problem in the experiment of present cell mar, can help the experimenter to easily accomplish straight marking line of waiting wide through using this cell mar ware, and simple and convenient, quick, can accomplish the cell mar experiment repeatedly.

The utility model discloses the technical scheme who takes provides a cell mar ware, its characterized in that: the device consists of a front buckle, a rear buckle, a central hollow groove, a horizontal panel and a graduated scale; the buckles are vertically arranged at the two ends of the horizontal panel; the central empty groove is arranged at the midpoint of the horizontal panel along the length direction; and a graduated scale is arranged on the edge of the central empty groove.

Preferably, the distance between the front and rear clasps matches the size of the cell culture plate.

Preferably, the distance between the front and rear catches is 83 mm.

Preferably, the height of the cell scarifier or the height of the front and back buckles is matched with the cell culture plate structure.

Preferably, the height of the cell scarifier or the height of the front buckle and the rear buckle is 5mm

Preferably, the width of the central hollow groove is matched with the diameter of the section of the pipette head corresponding to the tip of the pipette head when the tip of the pipette head touches the bottom of the plate.

Preferably, the width of the central hollow groove is 3mm to 3.5 mm.

Preferably, the bending piece is made of 304 stainless steel.

Compared with the prior art, the utility model discloses following beneficial effect has:

1. the scratch line that uses this cell mar ware to draw out in cell mar experiment is straight aequilate, effectively solves the scratch line that current scratch experiment appears easily and skews and the scheduling problem that the width differs.

2. Simple structure, low manufacturing cost, repeated use, economy and environmental protection.

Drawings

Fig. 1 is a front view of a cell streaking device according to an embodiment of the present invention.

Fig. 2 is a perspective view of a cell streaking device according to an embodiment of the present invention.

Detailed Description

The embodiments of the present invention will be further explained with reference to the drawings.

As shown in fig. 1 and fig. 2: a cell scratching device is a rectangular thin plate bending piece integrally and consists of a front buckle 4, a rear buckle 4, a central empty groove 2, a horizontal panel 1 and a graduated scale 3; the buckles 4 are vertically arranged at the two ends of the horizontal panel 1; the central empty groove 2 is arranged at the midpoint of the horizontal panel 1 along the length direction; and a graduated scale 3 is arranged on the edge of the central empty groove 2. Wherein, distance b between front and back buckle 4 is 83mm, and width a is 20mm, and buckle 4 height e is 5mm, and central cavity length c is 80mm, and width d is 3 ~ 3.5 mm. The thickness of each part of the cell scratching device is 0.5-1 mm, and the contact condition of the gun head and the plate bottom cannot be influenced under the thickness.

The using process of the invention is as follows: place the cell culture board that will prepare and wait to do the mar experiment on super clean bench, detain the cell mar ware in cell culture board top, because the distance between buckle 4 around the cell mar ware and the minor diameter length phase-match of cell culture board, so buckle 4 can be just with cell mar ware card firmly in cell culture board top around the back to guarantee can not take place the displacement at the in-process cell mar ware of scratching. Then vertically inserting the prepared pipette tip into the central hollow groove 2 of the cell scratching device and touching the bottom of the culture plate, wherein the pipette tip is just clamped due to the fact that the diameter of the pipette tip clamped in the central hollow groove 2 is matched with the groove width of the central hollow groove 2, and therefore the pipette tip cannot move left and right or up and down in the scratching process. After the gun head touches the bottom of the culture plate and is clamped tightly, the gun head is held by a hand to slowly slide along the central hollow groove 2 at a proper speed and force, and the gun head is scratched downwards according to the scale marks of the graduated scale 3 beside the central hollow groove 2. And after the scratch length meets the requirement, pulling out the gun head upwards, discarding the gun head, and repeating the steps by using a new gun head.

The width of the central hollow groove is manufactured according to the diameter of the clamped circular section of the pipette tip clamped in the central hollow groove when the pipette tip of the 200 mul pipette gun most commonly used in laboratories touches the bottom of a culture plate. The height of the cell scratching device or the height of the front and back buckling structures is manufactured according to the distance between a horizontal panel and the bottom surface of the culture plate when a pipette tip of a 200-mul pipette gun most commonly used in laboratories touches the bottom of the culture plate and is clamped in a central empty groove. Through tests, the width of the central empty groove and the height of the buckle structure provided by the invention can be well compatible with pipette tips of a plurality of manufacturers, so that the pipette tips can contact the bottom of the culture plate after being inserted, and the pipette body is vertical and does not shake.

It will be understood that, although the terms "front," "back," etc. may be used herein to describe various elements, these elements should not be limited by these terms. These terms are only used to distinguish one element from another. For example, a front element may be referred to as a back element, and similarly a back element may be referred to as a front element, without departing from the scope of the exemplary embodiments.

While the invention has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention. Those skilled in the art can make various changes, modifications and equivalent arrangements, which are equivalent to the embodiments of the present invention, without departing from the spirit and scope of the present invention, and which may be made by utilizing the techniques disclosed above; meanwhile, any changes, modifications and variations of the above-described embodiments, which are equivalent to those of the technical spirit of the present invention, are within the scope of the technical solution of the present invention.

Claims

1. A cell streaking device, comprising: the device consists of a front buckle (4), a rear buckle (4), a central empty groove (2), a horizontal panel (1) and a graduated scale (3); the buckles (4) are vertically arranged at the two ends of the horizontal panel (1); the central empty groove (2) is arranged at the midpoint of the horizontal panel (1) along the length direction; a graduated scale (3) is arranged on the edge of the central empty groove (2).

2. The cell streaker device as in claim 1 wherein: the distance between the front buckle and the rear buckle of the front buckle and the rear buckle (4) is matched with the size of the cell culture plate.

3. The cell streaker device as in claim 2 wherein: the distance between the front buckle and the rear buckle (4) is 83 mm.

4. The cell streaker device as in claim 1 wherein: the height of the cell scarifier or the height of the front buckle and the rear buckle (4) is matched with the cell culture plate structure.

5. The cell streaker device as in claim 4 wherein: the height of the cell scarifier or the height of the front buckle and the rear buckle (4) is 5 mm.

6. The cell streaker device as in claim 1 wherein: the width of the central empty groove (2) is matched with the diameter of the pipette head of the pipette.

7. The cell streaker device as in claim 6 wherein: the width of the central empty groove (2) is 3-3.5 mm.