CN108469497B - Experimental device for determining toxicity of spider mites - Google Patents
Experimental device for determining toxicity of spider mites Download PDFInfo
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- CN108469497B CN108469497B CN201810228196.8A CN201810228196A CN108469497B CN 108469497 B CN108469497 B CN 108469497B CN 201810228196 A CN201810228196 A CN 201810228196A CN 108469497 B CN108469497 B CN 108469497B
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- 241001454295 Tetranychidae Species 0.000 title abstract description 43
- 231100000419 toxicity Toxicity 0.000 title abstract description 10
- 230000001988 toxicity Effects 0.000 title abstract description 10
- 239000011521 glass Substances 0.000 claims abstract description 26
- 230000001018 virulence Effects 0.000 claims abstract description 15
- 238000004088 simulation Methods 0.000 claims abstract description 9
- 241000239290 Araneae Species 0.000 claims abstract description 8
- 238000012360 testing method Methods 0.000 claims description 59
- 241001454294 Tetranychus Species 0.000 claims description 15
- 239000007921 spray Substances 0.000 claims description 15
- 230000007704 transition Effects 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 231100000820 toxicity test Toxicity 0.000 claims description 11
- 238000007789 sealing Methods 0.000 claims description 8
- 238000003491 array Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 5
- 238000000889 atomisation Methods 0.000 claims description 4
- 229920005372 Plexiglas® Polymers 0.000 claims 1
- 239000004926 polymethyl methacrylate Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 11
- 238000002474 experimental method Methods 0.000 abstract description 9
- 206010059866 Drug resistance Diseases 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 6
- 238000011161 development Methods 0.000 abstract description 5
- 238000005286 illumination Methods 0.000 abstract description 5
- 230000002045 lasting effect Effects 0.000 abstract description 3
- 231100000518 lethal Toxicity 0.000 abstract description 3
- 230000001665 lethal effect Effects 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 abstract description 2
- 238000005457 optimization Methods 0.000 description 9
- 239000002435 venom Substances 0.000 description 5
- 231100000611 venom Toxicity 0.000 description 5
- 210000001048 venom Anatomy 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 241001454293 Tetranychus urticae Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
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- 239000000243 solution Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to a spider mite virulence determination experimental device, which comprises four box bodies, wherein each box body is a cube formed by glass plates on the front, the back, the left and the right sides, a plurality of simulation lamps are arranged on the glass plate on the upper part of the front side of each box body, the simulation lamps are arranged on a lamp holder frame on the top of each box body, an opening and closing door is arranged on the lower part of the front side of each box body, a plurality of atomizing nozzles are arranged on the glass plate on the rear side of each box body, the atomizing nozzles are connected with atomizing air pipelines, and the atomizing air; the device of the invention not only can feed and breed the spider mites, but also can carry out toxicity determination experiments on the spider mites. And the drug resistance of the spider mites is measured and analyzed to obtain the lethal middle concentration, sublethal concentration, maximum lasting period and other data of the spider mites, so that the method is favorable for developing the work of controlling the spider mites and has great significance for the development of the green planting industry. The device can control the humidity, temperature, illumination, gas atmosphere and other conditions of each box body, and further finely adjusts the growth and toxicity measurement experiment process of the spider mites.
Description
Technical Field
The invention relates to insect culture, in particular to a tetranychus toxicity test experimental device.
Background
With the rapid development of national economy, the green planting industry expands rapidly, so that the requirement of cultivation area is greatly increased, cultivation conditions are diversified, and more challenges are faced. Insect pests are a big problem in the complicated planting process, and the problems of the conventional large-size insect pest cause body types and the like can be solved by physical means, chemical means and crude drug means. The microminiaturization of insect pests needs to be solved by some high-precision killing means, such as the application of pesticide by gene cutting to prevent and control spider mites. However, this type of approach requires a large number of experimental samples to be tested for optimal results. Therefore, large-scale standardized breeding and propagation of the tetranychus urticae are needed, and a special experimental device is used for carrying out virulence determination experiments on the tetranychus urticae, but the prior technology and equipment have the following problems: (1) the existing device can not simultaneously carry out the tetranychus feeding and breeding and the toxicity test experiment of tetranychus, needs two sets of devices to realize the experiment, and has high cost and difficult tetranychus migration process. And the data samples obtained by the existing virulence determination experimental device are few, and the significance of the control guidance of the spider mites is not great. (2) The existing device cannot mark the spider mites, is not beneficial to counting survival and death numbers of the spider mites in a subsequent toxicity determination experiment, and has inaccurate experimental data. (3) The existing device cannot carry out real-time observation, and meanwhile, has no control means matched with related conditions, such as humidity, temperature, illumination, gas atmosphere and the like which can influence the growth rate and the survival condition in the virulence determination experiment process, and cannot carry out targeted fine adjustment because the growth or death condition of the tetranychid cannot be obtained in real time. Therefore, aiming at the problems, designing and developing a tetranychus toxicity test experimental device is in urgent need.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide an experimental device for determining the toxicity of tetranychus urticae.
In order to achieve the purpose, the invention adopts the following technical scheme:
the spider mite virulence determination experimental device comprises four box bodies, wherein each box body is a cube formed by glass plates on the front, the back, the left and the right sides, a plurality of simulation lamps are arranged on the glass plate on the upper part of the front side of each box body, the simulation lamps and a lamp holder frame arranged at the top of each box body are arranged, an opening and closing door is arranged at the lower part of the front side of each box body, a plurality of atomizing nozzles are arranged on the glass plate on the rear side of each box body, the atomizing nozzles are connected with atomizing pipelines, and the atomizing pipelines are connected with an atomizer;
a plurality of temperature control rod arrays are arranged on a bottom plate of the box body, the temperature control rod arrays are connected with a temperature control machine table arranged at the bottom of the box body, and a time duration display plate is arranged at the top of the box body; an atmosphere circulating pipe is arranged on the glass plate on the left side of the box body, and two ends of the atmosphere circulating pipe are respectively connected with the air inlet machine and the air pump;
the four box bodies are connected in pairs through transition bridges, layered plates are arranged in the transition bridges, and sealing doors are arranged at the tops of the transition bridges;
the four box bodies are composed of an incubator, a first test box, a second test box and a third test box which are connected in sequence, a liquid tank is arranged at the top of the incubator, a marking spray head is connected to the bottom of the liquid tank, and one end of the marking spray head is positioned in the incubator;
the upper end and the lower end of the top plate of the first test box, the upper end and the lower end of the top plate of the second test box and the upper end and the lower end of the top plate of the third test box are respectively provided with a signal acquisition instrument and a track frame, the track frame is provided with a scanning travelling crane, and the signal acquisition instrument is electrically connected with the scanning travelling crane; the glass plate on first test box, second test box, third test box right side is provided with a plurality of injection pipes of puting in, put in the spray pipe and put in the groove machine and be connected, it is fixed that the groove machine of puting in passes through the support.
According to the further optimization of the technical scheme, the bottom ends of the incubator, the first test box, the second test box and the third test box are provided with supporting legs.
In the further optimization of the technical scheme, the glass plate material is made of resin glass.
According to the further optimization of the technical scheme, handles are arranged on the opening and closing door and the sealing door.
According to the further optimization of the technical scheme, the middle section of the atmosphere circulating pipe is provided with a plurality of air holes.
According to the further optimization of the technical scheme, one end of the mark spray head is provided with a spray hole.
According to the further optimization of the technical scheme, the LED panels are arranged on the two sides of the time duration display panel.
According to the further optimization of the technical scheme, the layering plate is provided with a strip hole.
According to the further optimization of the technical scheme, the oscillation atomization module and the volume scales are arranged on the throwing groove machine.
According to the further optimization of the technical scheme, the throwing nozzle is provided with a nozzle, and the throwing nozzle is of a telescopic deformation structure capable of being embedded and sleeved.
The invention has the beneficial effects that:
(1) the device of the invention not only can feed and breed the spider mites, but also can carry out toxicity determination experiments on the spider mites. And the statistical analysis is carried out on the drug resistance of the spider mites to obtain the data of lethal medium concentration, sublethal concentration, maximum lasting period and the like of the spider mites, which is beneficial to developing the work of preventing and controlling the spider mites and has great significance for the development of green planting industry.
(2) The device provided by the invention is used for marking the tetranychus in the incubator by spraying the marking liquid, so that the scanning statistics of survival and death numbers of the tetranychus in a subsequent toxicity determination experiment of drug resistance is facilitated, and the accuracy of experimental data is ensured.
(3) The device can observe and judge the growth condition of the spider mites in the incubator and the drug resistance condition of the spider mites after poisoning in the three test boxes in real time through the glass plate, and meanwhile, the device can control the conditions of humidity, temperature, illumination, gas atmosphere and the like of each box body, so that the experimental process of the growth and toxicity measurement of the spider mites is finely adjusted.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a first schematic view of the incubator and associated components of the present invention;
FIG. 3 is a schematic structural diagram of a marking nozzle and a liquid bath according to the present invention;
FIG. 4 is a schematic view of the structure of the atmosphere recycling tube and associated components of the present invention;
FIG. 5 is a schematic diagram of the structure of an analog lamp and associated components of the present invention;
FIG. 6 is a schematic view of the construction of an atomizing nozzle and associated components of the present invention;
FIG. 7 is a schematic diagram of the structure of the time duration display panel of the present invention;
FIG. 8 is a schematic structural view of a temperature control rod array and a temperature control machine according to the present invention;
FIG. 9 is a second schematic view of the incubator and related components of the present invention;
FIG. 10 is a schematic structural view of a transition bridge and associated components of the present invention;
FIG. 11 is a first schematic view of the first test chamber and related components of the present invention;
FIG. 12 is a schematic structural view of the dispensing spout and associated components of the present invention;
FIG. 13 is a schematic structural view of a scanning carriage and associated components of the present invention;
FIG. 14 is a second schematic structural view of the first testing chamber and related components of the present invention;
the system comprises an incubator 1, a glass plate 2, an opening and closing door 3, a temperature control rod array 4, a temperature control machine table 5, an atmosphere circulating pipe 6, an air inlet machine 7, an air extractor 8, an atomizing nozzle 9, an atomizing air pipeline 10, an atomizing machine 11, a simulation lamp 12, a lamp holder frame 13, a marking spray head 14, a liquid tank 15, a duration display panel 16, a transition bridge 17, a layering plate 18, a sealing door 19, a first test box 20, a scanning traveling crane 21, a track frame 22, a signal acquisition instrument 23, a support 24, a throwing groove machine 25, a throwing spray pipe 26, a second test box 27 and a third test box 28.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in FIGS. 1-14: the spider mite virulence determination experimental device comprises four box bodies, wherein each box body is a cube formed by glass plates 2 on the front, the back, the left and the right sides, a plurality of simulation lamps 12 are arranged on the glass plate 2 on the upper part of the front side of each box body, the simulation lamps 12 are connected with a lamp holder frame 13 arranged at the top of each box body, an opening and closing door 3 is arranged on the lower part of the front side of each box body, a plurality of atomizing nozzles 9 are arranged on the glass plate 2 on the rear side of each box body, the atomizing nozzles 9 are connected with an atomizing gas pipeline 10, and the atomizing gas pipeline 10 is connected with an atomizer 11;
a plurality of temperature control rod arrays 4 are arranged on a bottom plate of the box body, the temperature control rod arrays 4 are connected with a temperature control machine table 5 arranged at the bottom of the box body, and a time duration display plate 16 is arranged at the top of the box body; an atmosphere circulating pipe 6 is arranged on the glass plate 2 on the left side of the box body, and two ends of the atmosphere circulating pipe 6 are respectively connected with an air inlet machine 7 and an air extractor 8;
the four box bodies are connected pairwise through a transition bridge 17, a layered plate 18 is arranged in the transition bridge 17, and a sealing door 19 is arranged at the top of the transition bridge 17;
the four box bodies are composed of an incubator 1, a first test box 20, a second test box 27 and a third test box 28 which are connected in sequence, a liquid tank 15 is arranged at the top of the incubator 1, a mark spray head 14 is connected to the bottom of the liquid tank 15, and one end of the mark spray head 14 is positioned in the incubator 1;
the upper end and the lower end of the top plate of the first test box 20, the second test box 27 and the third test box 28 are respectively provided with a signal acquisition instrument 23 and a track frame 22, the track frame 22 is provided with a scanning travelling crane 21, and the signal acquisition instrument 23 is electrically connected with the scanning travelling crane 21; the glass plate 2 on the right side of the first test box 20, the second test box 27 and the third test box 28 is provided with a plurality of throwing jet pipes 26, the throwing jet pipes 26 are connected with a throwing groove machine 25, and the throwing groove machine 25 is fixed through a support 24.
The bottom ends of the incubator 1, the first test box 20, the second test box 27 and the third test box 28 are all provided with supporting legs.
The glass plate 2 is made of resin glass.
Handles are arranged on the opening and closing door 3 and the sealing door 19.
And a plurality of air holes are formed in the middle section of the atmosphere circulating pipe 6.
The marker spray head 14 is provided with a spray hole at one end.
The duration display panel 16 is provided with LED panels on both sides.
The layered plate 18 is provided with a plurality of holes.
And the throwing groove machine 25 is provided with a vibration atomization module and volume scales. The groove feeding machine 25 is used for placing the venom, and the vibration atomization module is favorable for uniformly mixing the venom.
The throwing nozzle 26 is provided with a nozzle, and the throwing nozzle 26 is of an embedded telescopic deformation structure. Is beneficial to the even administration of the venom and ensures that all spider mites occupy the venom.
When the invention works: the method comprises the following steps of putting spider mite larvae into an incubator 1 through an opening and closing door 3 for culture, filling a marking liquid into a liquid tank 15, spraying the marking liquid to the spider mite larvae in the incubator 1 through a marking spray head 14 for marking so as to facilitate the subsequent scanning of the statistics of the number of spider mites, and guiding the spider mites into a first test box 20 through a transition bridge 17 after the spider mites are cultured in the incubator 1 to a certain stage; the method comprises the following steps that spider mites are subjected to first-stage virulence determination in a first test box 20, firstly, the number of the spider mites in the first test box 20 is scanned through a scanning travelling crane 21, information of the number of the live spider mites is fed back to a signal acquisition instrument 23, then a certain amount of venom is fed into the first test box 20 through a feeding spray pipe 26, timing is conducted through a duration display board 16, the death condition of the spider mites is observed, after a certain period of time, the live spider mites are guided into a second test box 27 through a transition bridge 17, the dead spider mites are left in the first test box 20, then the number of the dead spider mites in the first test box 20 are scanned through the scanning travelling crane 21 again, the death information of the live spider mites is fed back to the signal acquisition instrument 23, and the first-stage virulence determination of the spider mites is completed; the tetranychid which survives the first stage virulence determination is subjected to tetranychid second stage virulence determination in a second test box 27, and the steps are the same as the first stage virulence determination; the spider mites which survive the second-stage virulence determination are subjected to a third-stage virulence determination of spider mites in a third test box 28, the steps being as described above; finally, the survival number, death number, poison input amount, test duration and the like of the spider mites in each stage are counted, and the statistical analysis is carried out on the drug resistance of the spider mites, so that the data of lethal medium concentration, sublethal concentration, maximum lasting period of the medicament and the like of the spider mites can be finally obtained, the development of spider mite control work is facilitated, and the method has great significance for the development of green planting industry.
The growth condition of the spider mites in the incubator 1 and the drug resistance condition of the spider mites after poisoning in the three test boxes can be observed and judged in real time through the glass plate 2, and meanwhile, the device can control the humidity, temperature, illumination, gas atmosphere and other conditions of the incubator 1, the first test box 20, the second test box 27 and the third test box 28, so that the experimental process of measuring the growth and toxicity of the spider mites is finely adjusted. The method specifically comprises the following steps: the temperature of the box body is controlled by combining the temperature control rod array 4 and the temperature control machine table 5; the control of the gas atmosphere in the box body is realized by combining the culture atmosphere circulating pipe 6, the gas inlet machine 7 and the gas pumping machine 8; the humidity in the box body is controlled by combining the atomizing nozzle 9, the atomizing air pipeline 10 and the atomizer 11; the control of the illumination intensity in the box body is realized through the analog lamp 12.
The dead spider mites in the first test box 20, the second test box 27 and the third test box 28 can be cleared through the opening and closing door 3. When the transitional bridge 17 guides the spider mites, fine crushed leaves or slurry are put into the transitional bridge 17 through the sealing door 19, the fine crushed leaves stay in the laminated plate 18, and the slurry flows into the bottom of the transitional bridge 17 through the strip holes of the laminated plate 18.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (10)
1. The spider mite virulence determination experimental device comprises four box bodies and is characterized in that the box bodies are cubes formed by glass plates (2) on the front, the back, the left and the right sides, a plurality of simulation lamps (12) are arranged on the glass plates (2) on the upper portion of the front side of the box bodies, the simulation lamps (12) are arranged on lamp holders (13) on the tops of the box bodies, an opening and closing door (3) is arranged on the lower portion of the front side of the box bodies, a plurality of atomizing nozzles (9) are arranged on the glass plates (2) on the rear side of the box bodies, the atomizing nozzles (9) are connected with atomizing gas pipelines (10), and the atomizing gas pipelines (10) are connected with an atomizer (11);
a plurality of temperature control rod arrays (4) are arranged on a bottom plate of the box body, the temperature control rod arrays (4) are connected with a temperature control machine table (5) arranged at the bottom of the box body, and a time duration display plate (16) is arranged at the top of the box body; an atmosphere circulating pipe (6) is arranged on the glass plate (2) on the left side of the box body, and two ends of the atmosphere circulating pipe (6) are respectively connected with an air inlet machine (7) and an air pump (8);
the four box bodies are connected with each other through a transition bridge (17), a layered plate (18) is arranged in the transition bridge (17), and a sealing door (19) is arranged at the top of the transition bridge (17);
the four box bodies are composed of an incubator (1), a first test box (20), a second test box (27) and a third test box (28) which are connected in sequence, a liquid tank (15) is arranged at the top of the incubator (1), a marking spray head (14) is connected to the bottom of the liquid tank (15), and one end of the marking spray head (14) is positioned in the incubator (1);
the upper end and the lower end of a top plate of the first test box (20), the upper end and the lower end of a top plate of the second test box (27) and the upper end and the lower end of a top plate of the third test box (28) are respectively provided with a signal acquisition instrument (23) and a track frame (22), the track frame (22) is provided with a scanning travelling crane (21), and the signal acquisition instrument (23) is electrically connected with the scanning travelling crane (21); be provided with a plurality of spouts (26) of puting in on glass sheet (2) on first test box (20), second test box (27), third test box (28) right side, put in spout (26) and put in groove machine (25) and be connected, it is fixed through support (24) to put in groove machine (25).
2. The tetranychus toxicity test experimental apparatus according to claim 1, wherein the bottom ends of the incubator (1), the first test box (20), the second test box (27) and the third test box (28) are provided with supporting legs.
3. The tetranychus toxicity test experimental apparatus according to claim 1, wherein the glass plate (2) is made of plexiglas.
4. A tetranychus toxicity test experimental apparatus as claimed in claim 1, wherein the opening/closing door (3) and the sealing door (19) are provided with handles.
5. The tetranychus toxicity test experimental apparatus according to claim 1, wherein a plurality of air holes are formed in the middle section of the atmosphere circulating pipe (6).
6. The tetranychus toxicity test experimental apparatus as claimed in claim 1, wherein one end of the mark nozzle (14) is provided with a spraying hole.
7. A spider mite virulence determination experimental apparatus according to claim 1, wherein LED panels are arranged on both sides of the time duration display panel (16).
8. A tetranychus toxicity test apparatus as claimed in claim 1, wherein the layered plate (18) is provided with a plurality of holes.
9. The tetranychus toxicity test experimental apparatus as claimed in claim 1, wherein the throwing groove machine (25) is provided with a vibration atomization module and volume scales.
10. The tetranychus toxicity test experimental apparatus according to claim 1, wherein the spraying nozzle (26) is provided with a nozzle, and the spraying nozzle (26) is of a telescopic deformation structure capable of being nested.
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| CN201810228196.8A CN108469497B (en) | 2018-03-13 | 2018-03-13 | Experimental device for determining toxicity of spider mites |
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| CN201810228196.8A CN108469497B (en) | 2018-03-13 | 2018-03-13 | Experimental device for determining toxicity of spider mites |
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| CN108469497B true CN108469497B (en) | 2020-06-02 |
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| CN114794030B (en) * | 2022-06-16 | 2022-10-14 | 广东省农业科学院植物保护研究所 | Citrus psylla drug resistance monitoring device and drug resistance determination method |
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| KR20110014726A (en) * | 2009-08-06 | 2011-02-14 | 건국대학교 산학협력단 | Apparatus and method for extracting enchytraeidae from soil |
| CN201830771U (en) * | 2009-12-02 | 2011-05-18 | 农业部农药检定所 | Ventilation-type insect toxicity test culture device |
| CN202218572U (en) * | 2011-07-14 | 2012-05-16 | 华中农业大学 | Full automatic midge incubation box |
| US20140261188A1 (en) * | 2012-04-03 | 2014-09-18 | Byeong-Gyu Chin | Apparatus for breeding fly larvae |
| CN203446334U (en) * | 2013-08-22 | 2014-02-26 | 成都医学院第一附属医院 | Animal model casing |
| CN104165972B (en) * | 2014-08-14 | 2016-01-20 | 西南大学柑桔研究所 | Predatory Mites toxicity test method |
| CN104165972A (en) * | 2014-08-14 | 2014-11-26 | 中国农业科学院柑桔研究所 | Predatory mite toxicity testing method |
| CN204070204U (en) * | 2014-09-23 | 2015-01-07 | 福建国际旅行卫生保健中心 | Constant temperature raises the experimental provision of mosquito, test and the novel mosquito prevention and control of assessment |
| CN204259645U (en) * | 2014-11-07 | 2015-04-15 | 福建农林大学 | A kind of fruit bat beat exposure test Special artificial climate box |
| CN204259646U (en) * | 2014-11-20 | 2015-04-15 | 中国农业科学院柑桔研究所 | The device of artificial indoor rearing tetranychid |
| CN104770340A (en) * | 2015-03-27 | 2015-07-15 | 华南农业大学 | Method for determining stomach toxicity of oriental fruit flies by utilizing simple device |
| CN205408845U (en) * | 2016-03-28 | 2016-08-03 | 张德珍 | Plant incubator is used in child care |
| CN106370797A (en) * | 2016-11-16 | 2017-02-01 | 广西壮族自治区农业科学院植物保护研究所 | Device and method for determining toxicity of Bactrocera cucurbitae feeding pesticide |
| CN206609854U (en) * | 2017-03-30 | 2017-11-03 | 贵阳学院 | Yellow tea mite toxicity test device |
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