CN108469497A - Tetranychid toxicity test experimental provision - Google Patents
Tetranychid toxicity test experimental provision Download PDFInfo
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- CN108469497A CN108469497A CN201810228196.8A CN201810228196A CN108469497A CN 108469497 A CN108469497 A CN 108469497A CN 201810228196 A CN201810228196 A CN 201810228196A CN 108469497 A CN108469497 A CN 108469497A
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- 231100000820 toxicity test Toxicity 0.000 title claims abstract description 35
- 239000011521 glass Substances 0.000 claims abstract description 26
- 238000000889 atomisation Methods 0.000 claims abstract description 12
- 238000004088 simulation Methods 0.000 claims abstract description 8
- 238000012360 testing method Methods 0.000 claims description 58
- 239000007788 liquid Substances 0.000 claims description 11
- 238000009991 scouring Methods 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 230000007246 mechanism Effects 0.000 claims description 5
- 229920005372 Plexiglas® Polymers 0.000 claims description 3
- 238000003491 array Methods 0.000 claims description 3
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 8
- 241000607479 Yersinia pestis Species 0.000 abstract description 6
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 5
- 238000005286 illumination Methods 0.000 abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 4
- 241000238876 Acari Species 0.000 abstract description 3
- 230000002045 lasting effect Effects 0.000 abstract description 3
- 231100000636 lethal dose Toxicity 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 14
- 241000238631 Hexapoda Species 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000002435 venom Substances 0.000 description 4
- 231100000611 venom Toxicity 0.000 description 4
- 210000001048 venom Anatomy 0.000 description 4
- 101100165177 Caenorhabditis elegans bath-15 gene Proteins 0.000 description 3
- 231100000572 poisoning Toxicity 0.000 description 3
- 230000000607 poisoning effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Catching Or Destruction (AREA)
Abstract
The present invention relates to tetranychid toxicity test experimental provisions, including four babinets, babinet is the cube that all around four sides is made of glass plate, multiple simulation lamps are provided on the glass plate on cabinet front top, simulation lamp is equipped with switching door, multiple atomizers is provided on the glass plate on rear side of babinet with the lamp frame of seat in top of the box, cabinet front lower part is arranged, atomizer is connect with atomization feed channel, and atomization feed channel is connect with atomizer；The device of the invention not only can captive breeding tetranychid, can also to tetranychid carry out toxicity test experiment.And analysis is measured to the drug resistance of tetranychid, the lethal concentration of 50 of tetranychid, sublethal concentration, the data such as maximum lasting period of medicament are obtained, is conducive to carry out Control of Pest Mites work, be of great significance to the development of green planting industry.The present apparatus can control the conditions such as the humidity of each babinet, temperature, illumination, atmosphere, and then be fine-tuned to tetranychid growth and toxicity test experimentation.
Description
Technical field
The present invention relates to insect cultures, and in particular to tetranychid toxicity test experimental provision.
Background technology
With the high speed development of national economy, green planting industry explosion, so that acreage under cultivation demand is substantially
Increase, cultivates condition more diversification, face more challenges.And insect pest is to ask greatly in complicated planting process
The problems such as topic, conventional big build insect pest is because of build, can be solved by physical means, chemical means and crude drug means.And
Microminiaturization insect pest then need by some it is high-grade, precision and advanced kill means and solve, cut out dispenser using gene and prevent tetranychid etc..
But this kind of means, which needs a large amount of experiment sample to carry out experiment, can play optimum efficiency.So needing to carry out tetranychid big
The captive breeding of scale standard, and toxicity test experiment, but current technology are carried out to tetranychid using special experimental provision
The problem of there is the following aspects with equipment：(1) current device cannot be carried out at the same time tetranychid captive breeding and tetranychid
Toxicity test experiment, need two covering devices to realize, it is not only of high cost, and it is difficult to migrate tetranychid process.And with current
The obtained data sample of toxicity test experimental provision it is less, it is little to the prevention directive significance of tetranychid.(2) current device without
Tetranychid is marked in method, be unfavorable for tetranychid in the experiment of follow-up toxicity test survive and dead number statistics, experimental data is not
Precisely.(3) current device can not be observed in real time, while without the control means of mating correlated condition, such as humidity, temperature
Degree, illumination, atmosphere etc. can influence the condition of growth rate and Survival in toxicity test experimentation, and because can not be real
When obtain the situation of tetranychid growth or death, and then can not targetedly be fine-tuned.So in view of the above-mentioned problems, setting
Meter exploitation tetranychid toxicity test experimental provision becomes current urgent need.
Invention content
For problems of the prior art, the object of the present invention is to provide tetranychid toxicity test experimental provisions.
To achieve the goals above, the present invention adopts the following technical scheme that：
Tetranychid toxicity test experimental provision, including four babinets, the babinet are that all around four sides is made of glass plate
Cube, be provided with multiple simulation lamps on the glass plate on the cabinet front top, the simulation lamp be arranged on babinet top
The lamp frame of seat in portion, the cabinet front lower part are equipped with switching door, and multiple atomization sprays are provided on the glass plate on rear side of the babinet
Mouth, the atomizer are connect with atomization feed channel, and the atomization feed channel is connect with atomizer；
It is provided with multiple temperature control stick arrays in the box baseplate, the temperature control stick array and the control in the bottom of box is set
Warm board connection, the top of the box is provided with duration display board；Atmosphere cycle is provided on glass plate on the left of the babinet
Pipe, atmosphere circulation pipe both ends are connect with into mechanism of qi and air exhauster respectively；
Four babinets are connected by transit bridge between any two, and demixing plate, the transition are provided in the transit bridge
It is provided with hermatic door at the top of bridge；
Four babinets are made of sequentially connected incubator, the first test box, the second test box, third test box,
Liquid bath is provided at the top of the incubator, the liquid bath bottom is connected with label nozzle, and described label nozzle one end is located at culture
In case；
First test box, the second test box, third test roof box upper and lower ends are respectively arranged with signal sampler
And track frame, scanning driving is provided in the track frame, the signal sampler is electrically connected with scanning driving；Described first
Test box, the second test box are provided with multiple dispensing jet pipes on the glass plate on the right side of third test box, the dispensing jet pipe with throw
Scouring machine connection is put, the dispensing scouring machine is fixed by holder holder.
The technical program advanced optimizes, the incubator, the first test box, the second test box, third test box bottom
End is both provided with support leg.
The technical program advanced optimizes, and the glass plate material is plexiglas material.
The technical program advanced optimizes, and handle is both provided on the switching door and hermatic door.
The technical program advanced optimizes, and the atmosphere circulation pipe is provided with multiple stomatas on stage casing.
The technical program advanced optimizes, and described label nozzle one end is provided with spray hole.
The technical program advanced optimizes, and both sides are both provided with LED panel on the duration display board.
The technical program advanced optimizes, and a hole is provided on the demixing plate.
The technical program advanced optimizes, and oscillating-atomizing module and volume markings are provided on the dispensing scouring machine.
The technical program advanced optimizes, and is provided with spout on the dispensing jet pipe, and launch jet pipe be can nesting stretch
Compression deformation structure.
The beneficial effects of the present invention are：
(1) the device of the invention not only can captive breeding tetranychid, can also to tetranychid carry out toxicity test experiment.And to leaf
The drug resistance of mite is for statistical analysis, obtains the number such as maximum lasting period of the lethal concentration of 50 of tetranychid, sublethal concentration, medicament
According to, be conducive to carry out Control of Pest Mites work, be of great significance to the development of green planting industry.
(2) the device of the invention is marked by spraying marking fluid to the tetranychid in incubator, is conducive to follow-up anti-medicine
Property toxicity test experiment in tetranychid survive and the scan statistics of dead number, ensure that experimental data is accurate.
(3) the device of the invention can in real time be observed by glass plate judges in incubator the growing state of tetranychid and three
Drug resistance status in test box after tetranychid poisoning, while the present apparatus can be to the humidity of each babinet, temperature, illumination, gas atmosphere
It the conditions such as encloses to be controlled, and then tetranychid growth and toxicity test experimentation is fine-tuned.
Description of the drawings
Fig. 1 is the structural schematic diagram of the present invention；
Fig. 2 is the structural schematic diagram one of incubator and associated components of the present invention；
Fig. 3 is the structural schematic diagram of present invention label nozzle, liquid bath；
Fig. 4 is the structural schematic diagram of atmosphere circulation pipe and associated components of the present invention；
Fig. 5 is the structural schematic diagram of present invention simulation lamp and associated components；
Fig. 6 is the structural schematic diagram of atomizer and associated components of the present invention；
Fig. 7 is the structural schematic diagram of duration display board of the present invention；
Fig. 8 is the structural schematic diagram of temperature control stick array of the present invention, temperature control board；
Fig. 9 is the structural schematic diagram two of incubator and associated components of the present invention；
Figure 10 is the structural schematic diagram of transit bridge of the present invention and associated components；
Figure 11 is the structural schematic diagram one of the first test box and associated components of the invention；
Figure 12 is the structural schematic diagram that the present invention launches jet pipe and associated components；
Figure 13 is the structural schematic diagram of present invention scanning driving and associated components；
Figure 14 is the structural schematic diagram two of the first test box and associated components of the invention；
Wherein, incubator 1, glass plate 2, switching door 3, temperature control stick array 4, temperature control board 5, atmosphere circulation pipe 6, into mechanism of qi
7, air exhauster 8, atomizer 9, atomization feed channel 10, atomizer 11, simulation lamp 12, lamp frame of seat 13, label nozzle 14, liquid bath
15, duration display board 16, transit bridge 17, demixing plate 18, hermatic door 19, the first test box 20, scanning driving 21, track frame 22,
Signal sampler 23, holder 24 launch scouring machine 25, launch jet pipe 26, the second test box 27, third test box 28.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As shown in Fig. 1~14：Tetranychid toxicity test experimental provision, including four babinets, the babinet are all around four
The cube that face is made of glass plate 2 is provided with multiple simulation lamps 12, the mould on the glass plate 2 on the cabinet front top
Quasi- lamp 12 is equipped with switching door 3, the glass on rear side of the babinet with the lamp frame of seat 13 in top of the box, the cabinet front lower part is arranged
Be provided with multiple atomizers 9 in glass plate 2, the atomizer 9 connect with atomization feed channel 10, the atomization feed channel 10 and
Atomizer 11 connects；
It is provided with multiple temperature control stick arrays 4 in the box baseplate, the temperature control stick array 4 and is arranged in the bottom of box
Temperature control board 5 connects, and the top of the box is provided with duration display board 16；It is provided with gas on glass plate 2 on the left of the babinet
Atmosphere circulation pipe 6,6 both ends of atmosphere circulation pipe are connect with into mechanism of qi 7 and air exhauster 8 respectively；
Four babinets are connected by transit bridge 17 between any two, and demixing plate 18, institute are provided in the transit bridge 17
It states 17 top of transit bridge and is provided with hermatic door 19；
Four babinets are by sequentially connected incubator 1, the first test box 20, the second test box 27, third test box
28 are constituted, and 1 top of the incubator is provided with liquid bath 15, and 15 bottom of the liquid bath is connected with label nozzle 14, the label spray
First 14 one end is located in incubator 1；
First test box 20, the second test box 27,28 top plate upper and lower ends of third test box are respectively arranged with signal
Acquisition Instrument 23 and track frame 22 are provided with scanning driving 21 in the track frame 22, and the signal sampler 23 is driven a vehicle with scanning
21 are electrically connected；First test box 20, the second test box 27, the right side of third test box 28 glass plate 2 on be provided with it is more
A dispensing jet pipe 26, the dispensing jet pipe 26 are connect with scouring machine 25 is launched, and the dispensing scouring machine 25 is fixed by 24 holder of holder.
The incubator 1, the first test box 20, the second test box 27,28 bottom end of third test box are both provided with support leg.
2 material of the glass plate is plexiglas material.
It is both provided with handle on the switching door 3 and hermatic door 19.
It is provided with multiple stomatas on 6 stage casing of atmosphere circulation pipe.
Described 14 one end of label nozzle is provided with spray hole.
Both sides are both provided with LED panel on the duration display board 16.
A hole is provided on the demixing plate 18.
It is provided with oscillating-atomizing module and volume markings on the dispensing scouring machine 25.Scouring machine 25 is launched to be used to place venom,
Oscillating-atomizing module is conducive to venom and is uniformly mixed.
Be provided with spout on the dispensing jet pipe 26, and launch jet pipe 26 be can nested dilatation structure.Be conducive to poison
Liquid is launched uniformly, ensures that all tetranychids all occupy venom.
When the present invention works：Tetranychid larva is put by switching door 3 in incubator 1 and is cultivated, label is filled in liquid bath 15
Liquid is marked by marking tetranychid larva of the nozzle 14 into incubator 1 to spray marking fluid to facilitate follow-up tetranychid number to unite
Tetranychid is directed to the first test box by the scanning of meter after tetranychid is cultivated in incubator 1 to certain phase by transit bridge 17
In 20；Tetranychid carries out first stage toxicity test in the first test box 20, preferred to carry out the first test by scanning driving 21
The scanning of tetranychid number in case 20, and tetranychid number information will be survived and feed back to signal sampler 23, then by launching jet pipe
26 launch a certain amount of venom into the first test box 20, by 16 timing of duration display board, observe the death condition of tetranychid, centainly
After time, tetranychid will be survived by transit bridge 17 and be directed in the second test box 27, and dead tetranychid is left on the first test
In case 20, then again by the number of dead tetranychid in 21 the first test box 20 of scanning of scanning driving, and it is dead to survive tetranychid
It dies information and feeds back to signal sampler 23, complete tetranychid first stage degree toxicity test；By first stage degree toxicity test at
Tetranychid living carries out tetranychid second stage degree toxicity test, step and first stage toxicity test phase in the second test box 27
Together；The tetranychid survived by second stage degree toxicity test carries out the survey of tetranychid phase III degree virulence in third test box 28
Fixed, step is same as above；The tetranychid in each stage is finally survived number, dead number, the amount of poisoning, length of testing speech etc. to be united
Meter, and to for statistical analysis of the drug resistance of tetranychid, may finally obtain the lethal concentration of 50, sublethal concentration, medicine of tetranychid
The data such as the maximum lasting period of agent are conducive to carry out Control of Pest Mites work, be of great significance to the development of green planting industry.
The present invention can observation judges the growing state of tetranychid and three test boxs in incubator 1 in real time by glass plate 2
Drug resistance status after the poisoning of interior tetranychid, while the present apparatus can be to incubator 1, the first test box 20, the second test box 27, the
The conditions such as humidity, temperature, illumination, the atmosphere of three test boxs 28 are controlled, and then real to tetranychid growth and toxicity test
The process of testing is fine-tuned.Specially：The control to spin manifold temperature is implemented in combination with by temperature control stick array 4, temperature control board 5；
By training atmosphere circulation pipe 6, realizing the control to atmosphere in babinet into mechanism of qi 7,8 triplicity of air exhauster；Pass through atomization
The control to humidity in babinet is realized in nozzle 9, atomization feed channel 10,11 triplicity of atomizer；By simulating the realization pair of lamp 12
The control of intensity of illumination in babinet.
First test box 20, the second test box 27, tetranychid dead in third test box 28 can be clear by switching door 3
Go out.Transit bridge 17 guide tetranychid when leaf or slurry fine crushing are put into transit bridge 17 by hermatic door 19, leaf fine crushing is being layered
Plate 18 stops, and slurry flows into 17 bottom of transit bridge by the hole of demixing plate 18.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although with reference to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution recorded in example is modified or equivalent replacement of some of the technical features, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (10)
1. tetranychid toxicity test experimental provision, including four babinets, which is characterized in that the babinet is all around on four sides by glass
The cube that glass plate (2) is constituted is provided with multiple simulation lamps (12), the mould on the glass plate (2) on the cabinet front top
Quasi- lamp (12) and the lamp frame of seat (13) in top of the box is set, the cabinet front lower part is equipped with switching door (3), after the babinet
Multiple atomizers (9) are provided on the glass plate (2) of side, the atomizer (9) connect with atomization feed channel (10), described
Atomization feed channel (10) is connect with atomizer (11)；
It is provided with multiple temperature control stick arrays (4) in the box baseplate, the temperature control stick array (4) and is arranged in the bottom of box
Temperature control board (5) connects, and the top of the box is provided with duration display board (16)；It is set on glass plate (2) on the left of the babinet
It is equipped with atmosphere circulation pipe (6), atmosphere circulation pipe (6) both ends are connect with into mechanism of qi (7) and air exhauster (8) respectively；
Four babinets are connected by transit bridge (17) between any two, and demixing plate (18) is provided in the transit bridge (17),
It is provided with hermatic door (19) at the top of the transit bridge (17)；
Four babinets are tested by sequentially connected incubator (1), the first test box (20), the second test box (27), third
Case (28) is constituted, and is provided with liquid bath (15) at the top of the incubator (1), liquid bath (15) bottom is connected with label nozzle
(14), described label nozzle (14) one end is located in incubator (1)；
First test box (20), the second test box (27), third test box (28) top plate upper and lower ends are respectively arranged with letter
Number Acquisition Instrument (23) and track frame (22) are provided with scanning driving (21), the signal sampler on the track frame (22)
(23) it is electrically connected with scanning driving (21)；First test box (20), the second test box (27), third test box (28) are right
Multiple dispensing jet pipes (26) are provided on the glass plate (2) of side, the dispensing jet pipe (26) connect with scouring machine (25) is launched, described
Scouring machine (25) is launched to fix by holder (24) holder.
2. tetranychid toxicity test experimental provision according to claim 1, which is characterized in that the incubator (1), first are surveyed
Examination case (20), the second test box (27), third test box (28) bottom end are both provided with support leg.
3. tetranychid toxicity test experimental provision according to claim 1, which is characterized in that glass plate (2) material is
Plexiglas material.
4. tetranychid toxicity test experimental provision according to claim 1, which is characterized in that the switching door (3) and sealing
It is both provided with handle on door (19).
5. tetranychid toxicity test experimental provision according to claim 1, which is characterized in that in the atmosphere circulation pipe (6)
It is provided with multiple stomatas in section.
6. tetranychid toxicity test experimental provision according to claim 1, which is characterized in that described label nozzle (14) one end
It is provided with spray hole.
7. tetranychid toxicity test experimental provision according to claim 1, which is characterized in that on the duration display board (16)
Both sides are both provided with LED panel.
8. tetranychid toxicity test experimental provision according to claim 1, which is characterized in that be arranged on the demixing plate (18)
There is a hole.
9. tetranychid toxicity test experimental provision according to claim 1, which is characterized in that set on the dispensing scouring machine (25)
It is equipped with oscillating-atomizing module and volume markings.
10. tetranychid toxicity test experimental provision according to claim 1, which is characterized in that on the dispensing jet pipe (26)
Be provided with spout, and launch jet pipe (26) be can nested dilatation structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810228196.8A CN108469497B (en) | 2018-03-13 | 2018-03-13 | Experimental device for determining toxicity of spider mites |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810228196.8A CN108469497B (en) | 2018-03-13 | 2018-03-13 | Experimental device for determining toxicity of spider mites |
Publications (2)
| Publication Number | Publication Date |
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| CN108469497A true CN108469497A (en) | 2018-08-31 |
| CN108469497B CN108469497B (en) | 2020-06-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810228196.8A Expired - Fee Related CN108469497B (en) | 2018-03-13 | 2018-03-13 | Experimental device for determining toxicity of spider mites |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114794030A (en) * | 2022-06-16 | 2022-07-29 | 广东省农业科学院植物保护研究所 | Diaphorina citri drug resistance monitoring device and drug resistance determination method |
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| CN114794030A (en) * | 2022-06-16 | 2022-07-29 | 广东省农业科学院植物保护研究所 | Diaphorina citri drug resistance monitoring device and drug resistance determination method |
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