CN210656892U - Nucleic acid extraction kit for preventing cross contamination - Google Patents
Nucleic acid extraction kit for preventing cross contamination Download PDFInfo
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- CN210656892U CN210656892U CN201921376320.1U CN201921376320U CN210656892U CN 210656892 U CN210656892 U CN 210656892U CN 201921376320 U CN201921376320 U CN 201921376320U CN 210656892 U CN210656892 U CN 210656892U
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 34
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 34
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 34
- 238000012864 cross contamination Methods 0.000 title claims abstract description 28
- 238000000605 extraction Methods 0.000 title claims abstract description 24
- 238000007789 sealing Methods 0.000 claims abstract description 11
- 238000005096 rolling process Methods 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 6
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 230000008093 supporting effect Effects 0.000 description 5
- 238000009434 installation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 244000188472 Ilex paraguariensis Species 0.000 description 1
- 230000002337 anti-port Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
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Abstract
The utility model relates to the technical field of reagent kit, and discloses a nucleic acid extraction reagent kit for preventing cross contamination, which comprises a box body, wherein the box body is a hollow structure with an opening at the top, the opening position is connected with a matched box cover through a clamping mechanism, a matched mounting seat is arranged in the box body, a plurality of test tube grooves which are uniformly distributed are arranged on the upper surface of the mounting seat, matched test tubes are inserted in the test tube grooves, an elastic clamp is arranged between the test tubes and the notches of the test tube grooves, matched sealing plugs are fixedly connected at the positions of the lower surface of the box cover corresponding to the tube openings of the test tubes, the sealing plug extends into the test tube, a screw rod is rotatably connected in the box body through a bearing, one end of the screw rod, which is far away from the bearing, penetrates through the side wall of the box body and extends outwards, and the lead screw is rotationally connected with the side wall of the box body, two opposite threads are arranged on the lead screw, and the lead screw is arranged below the mounting seat. The utility model discloses conveniently carry out nucleic acid and connect the material, effectively avoid artifical too much contact and cause cross contamination.
Description
Technical Field
The utility model relates to a kit technical field especially relates to a prevent cross contamination's nucleic acid extraction kit.
Background
Nucleic acids include DNA and RNA, and nucleic acid extraction kits refer to reagents capable of extracting nucleic acids from a sample.
Traditional nucleic acid extraction test generally accomplishes through 96 deep-well plates, but traditional 96 deep-well plate is comparatively inconvenient in the use, can only stretch into to 96 deep-well plates through external reagent injection tube and take the nucleic acid of observing, and the manual hand is got to the reagent in reagent injection process is got and is put in addition, causes cross contamination easily.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the problem that the nucleic acid extraction in the prior art needs to be carried out the manual work to get the reagent and put, which easily causes the cross contamination, and the provided nucleic acid extraction kit for preventing the cross contamination.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a nucleic acid extraction kit preventing cross contamination comprises a box body, wherein the box body is of a hollow structure with an opening at the top, a matched box cover is connected to the opening position through a clamping mechanism, a matched mounting seat is arranged in the box body, a plurality of test tube grooves which are uniformly distributed are formed in the upper surface of the mounting seat, matched test tubes are inserted in the test tube grooves, an elastic clamp is arranged between the test tubes and the notches of the test tube grooves, matched sealing plugs are fixedly connected to the positions, corresponding to tube orifices of the test tubes, of the lower surface of the box cover and extend into the test tubes, a lead screw is rotatably connected in the box body through a bearing, one end, away from the bearing, of the lead screw penetrates through the side wall of the box body and extends outwards, the lead screw is rotatably connected with the side wall of the box body, two opposite-direction threads are arranged on the lead screw and located below the mounting seat, and two matched moving, the lower surface of the mounting seat is symmetrically hinged with two connecting rods, and the other ends of the connecting rods are hinged with the upper surfaces of the corresponding moving blocks.
Preferably, latch mechanism includes that the draw-in groove at lid both ends is seted up to two symmetries, the lateral wall of box body corresponds draw-in groove position department and has seted up the sliding tray, the tank bottom fixedly connected with spring of sliding tray, the fixture block that other end fixedly connected with and the draw-in groove of spring matees, and fixture block and sliding tray sliding connection, the one end that the spring was kept away from to the fixture block passes the notch of sliding tray and extends to the draw-in groove in and set up, the fixture block is close to one side fixedly connected with pull rod of sliding tray tank bottom, the tank bottom that the other end of pull rod passed the spring and run through the sliding tray outwards extends, and the tank bottom.
Preferably, the bottom symmetry fixedly connected with L type pole of mount pad, the other end fixedly connected with slider of L type pole, the spout that matches is seted up in the position department that the inner wall of box body corresponds the slider, the slider offsets with the lateral wall that the spout is close to the bottom, and slider and spout sliding connection.
Preferably, the lower surface of the moving block is fixedly connected with a moving rod, the bottom end of the moving rod is fixedly provided with a roller, the lower surface inside the box body is provided with a roller groove matched with the roller, and the roller is in rolling connection with the roller groove.
Preferably, one end of the screw rod, which is far away from the bearing, is fixedly connected with a rotating handle.
Preferably, the box body is symmetrically and fixedly connected with positioning blocks on the opposite inner walls close to the top, and the lower surface of the box cover is abutted to the upper surfaces of the positioning blocks.
Preferably, the upper surface of the box cover is fixedly connected with a handle.
Preferably, a first magnetic block is fixedly sleeved on the pull rod positioned outside the sliding groove, the outer side wall of the box body is fixedly connected with supporting rods which are symmetrical relative to the position of the pull rod, the other ends of the two supporting rods are fixedly connected with a second magnetic block together, the pull rod penetrates through the second magnetic block, and the pull rod is in sliding connection with the second magnetic block.
Preferably, one end of the pull rod, which is far away from the fixture block, is fixedly connected with a pull ring.
Preferably, one side of the sliding block, which is close to the bottom of the sliding groove, is provided with a ball groove, a ball is arranged in the ball groove, one end, which is far away from the bottom of the ball groove, of the ball groove penetrates through a notch of the ball groove and extends outwards, and the ball is connected to the bottom of the sliding groove in a rolling manner.
Compared with the prior art, the utility model provides a prevent cross contamination's nucleic acid extraction kit possesses following beneficial effect:
1. this prevent cross contamination's nucleic acid extraction kit, through setting up the box body, the lid, the mount pad, the test tube groove, the test tube, the elasticity presss from both sides, the sealing plug, the lead screw, movable block and connecting rod, the elasticity presss from both sides the stability that improves the test tube and place in the test tube groove, when needing to carry out nucleic acid injection, open the lid, the lead screw rotates, there are two opposite direction's screw thread on the lead screw, make two threaded connection's movable block move in opposite directions, drive articulated connecting rod, thereby make the mount pad rise in the box body, make things convenient for the test tube to connect the material, avoid artifical too much contact and cause cross contamination, after connecing the material, the antiport lead screw, drive the mount pad decline, make the test tube be located the box body, close the lid, seal the test tube through the sealing.
2. This prevent cross contamination's nucleic acid extraction kit, through setting up the box body, the lid, the draw-in groove, the sliding tray, a spring, fixture block and pull rod, the pulling is connected with the pull rod of fixture block, make fixture block and draw-in groove break away from, can conveniently separate lid and box body, the pulling pull rod, make the fixture block slide in the sliding tray and compression spring, arrange the lid in the box body, when the fixture block corresponds with the draw-in groove on the lid, loosen the pull rod, the spring takes place to reset, can make fixture block and draw-in groove block, accomplish the installation of lid, thereby the dismouting of convenient control lid.
The part that does not relate to in the device all is the same with prior art or can adopt prior art to realize, the utility model discloses conveniently carry out nucleic acid and connect the material, effectively avoid artifical too much contact and cause cross contamination.
Drawings
FIG. 1 is a schematic structural diagram of a nucleic acid extraction kit for preventing cross contamination according to the present invention;
FIG. 2 is an enlarged view of portion A of FIG. 1;
FIG. 3 is an enlarged view of portion B of FIG. 1;
fig. 4 is an enlarged view of a portion C in fig. 1.
In the figure: the test tube test device comprises a box body 1, a box cover 2, a mounting base 3, a test tube groove 4, a test tube 5, an elastic clamp 6, a sealing plug 7, a lead screw 8, a moving block 9, a connecting rod 10, a clamping groove 11, a sliding groove 12, a spring 13, a clamping block 14, a pull rod 15, a 16L-shaped rod, a slide block 17, a slide groove 18, a moving rod 19, a roller 20, a roller groove 21, a rotating handle 22, a positioning block 23, a handle 24, a first magnetic block 25, a support rod 26, a second magnetic block 27, a pull ring 28, a ball groove 29 and balls 30.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Referring to fig. 1-4, a nucleic acid extraction kit for preventing cross contamination, comprising a box body 1, wherein the box body 1 is a hollow structure with an open top, the open position is connected with a matched box cover 2 through a clamping mechanism, a matched mounting seat 3 is arranged in the box body 1, the upper surface of the mounting seat 3 is provided with a plurality of uniformly distributed test tube grooves 4, matched test tubes 5 are inserted in the test tube grooves 4, an elastic clamp 6 is arranged between the test tubes 5 and the notches of the test tube grooves 4, a matched sealing plug 7 is fixedly connected to the lower surface of the box cover 2 at the position corresponding to the pipe orifices of the test tubes 5, the sealing plug 7 extends into the test tubes 5, a screw rod 8 is rotatably connected to the box body 1 through a bearing, one end of the screw rod 8, which is far away from the bearing, penetrates through the side wall of the box body 1 and extends outwards, the screw rod 8 is rotatably connected to, the screw rod 8 is arranged below the mounting seat 3, the screw rod 8 is symmetrically in threaded connection with two matched moving blocks 9, the lower surface of the mounting seat 3 is symmetrically hinged with two connecting rods 10, the other ends of the connecting rods 10 are hinged with the upper surfaces of the corresponding moving blocks 9, the elastic clamp 6 effectively improves the stability of placing the test tube 5 in the test tube groove 4, when nucleic acid injection is needed, the box cover 2 is opened, the screw rod 8 is rotated, the screw rod 8 is provided with two threads with opposite directions, the two threaded moving blocks 9 move oppositely to drive the hinged connecting rods 10, so that the mounting seat 3 rises in the box body 1, the test tube 5 is convenient to receive materials, cross contamination caused by excessive manual contact is avoided, after the material receiving is finished, the screw rod 8 is reversely rotated to drive the mounting seat 3 to fall, the test tube 5 is located in the box body 1, the box cover 2 is closed, and the test tube 5 is sealed through the, contamination of the nucleic acid in the test tube 5 can be effectively avoided.
The clamping mechanism comprises two clamping grooves 11 symmetrically arranged at two ends of the box cover 2, a sliding groove 12 is arranged at the position of the side wall of the box body 1 corresponding to the clamping groove 11, a spring 13 is fixedly connected with the bottom of the sliding groove 12, a clamping block 14 matched with the clamping groove 11 is fixedly connected with the other end of the spring 13, the clamping block 14 is in sliding connection with the sliding groove 12, one end of the clamping block 14 far away from the spring 13 penetrates through the notch of the sliding groove 12 and extends into the clamping groove 11, a pull rod 15 is fixedly connected with one side of the clamping block 14 close to the bottom of the sliding groove 12, the other end of the pull rod 15 penetrates through the spring 13 and extends outwards through the bottom of the sliding groove 12, the pull rod 15 is in sliding connection with the bottom of the sliding groove 12, the pull rod 15 connected with the clamping block 14 is pulled to separate the clamping block 14 from the clamping groove 11, the box cover 2 can be conveniently separated from, the box cover 2 is arranged in the box body 1, when the clamping block 14 corresponds to the clamping groove 11 on the box cover 2, the pull rod 15 is loosened, the spring 13 is reset, the clamping block 14 can be clamped with the clamping groove 11, the installation of the box cover 2 is completed, and therefore the disassembly and assembly of the box cover 2 are conveniently controlled.
The bottom symmetry fixedly connected with L type pole 16 of mount pad 3, the other end fixedly connected with slider 17 of L type pole 16, the spout 18 that matches is seted up to the position department that the inner wall of box body 1 corresponds slider 17, slider 17 offsets with the lateral wall that spout 18 is close to the bottom, and slider 17 and 18 sliding connection of spout, improve the stability that mount pad 3 goes up and down, avoid taking place the skew at the lift in-process, and when slider 17 descends and conflicts with the 18 lateral walls of spout, mount pad 3 is in the state that resets, the convenient lift resets.
The lower surface of the moving block 9 is fixedly connected with a moving rod 19, the bottom end of the moving rod 19 is fixedly provided with a roller 20, the lower surface inside the box body 1 is provided with a roller groove 21 matched with the roller 20, and the roller 20 is in rolling connection with the roller groove 21, so that the guiding and supporting effects are achieved, and the stability is ensured.
One end of the screw rod 8 far away from the bearing is fixedly connected with a rotating handle 22, so that the rotation of the screw rod 8 is conveniently controlled.
The positioning blocks 23 are symmetrically and fixedly connected to the inner walls, close to the top, of the box body 1, the lower surface of the box cover 2 abuts against the upper surfaces of the positioning blocks 23, and when the box cover 2 abuts against the positioning blocks 23, the clamping blocks 14 correspond to the clamping grooves 11, so that the box cover 2 is convenient to install and position.
The upper surface of the box cover 2 is fixedly connected with a handle 24, which is convenient for taking the box cover 2.
The first magnetic block 25 is fixedly sleeved on the pull rod 15 positioned on the outer side of the sliding groove 12, the supporting rods 26 which are symmetrical relative to the position of the pull rod 15 are fixedly connected on the outer side wall of the box body 1, the second magnetic block 27 is fixedly connected to the other ends of the two supporting rods 26 together, the pull rod 15 penetrates through the second magnetic block 27, the pull rod 15 is slidably connected with the second magnetic block 27, when the pull rod 15 is pulled to separate the clamping block 14 from the clamping groove 11, the first magnetic block 25 on the pull rod 15 is abutted to the second magnetic block 27, attraction is generated between the first magnetic block 25 and the second magnetic block 27, and is greater than the tension of the spring 13, when the pull rod 15 is loosened, the spring 13 cannot be reset, the situation that the box cover 2 needs to pull the pull rod 15 all the time to avoid the reset of the spring 13 in the.
One end of the pull rod 15 far away from the fixture block 14 is fixedly connected with a pull ring 28, so that the pull rod 15 is conveniently driven to move.
One side of the sliding block 17 close to the bottom of the sliding groove 18 is provided with a ball groove 29, a ball 30 is arranged in the ball groove 29, one end, far away from the bottom of the ball groove 29, of the ball 30 penetrates through the notch of the ball groove 29 and extends outwards, the ball is connected to the bottom of the sliding groove 18 in a rolling mode, and friction force between the sliding block 17 and the sliding groove 18 is reduced.
In the utility model, the elastic clamp 6 effectively improves the stability of placing the test tube 5 in the test tube groove 4, when nucleic acid injection is needed, the box cover 2 is opened, the lead screw 8 is rotated, two opposite screw threads are arranged on the lead screw 8, the two moving blocks 9 connected by screw threads move oppositely to drive the hinged connecting rod 10, so that the mounting seat 3 rises in the box body 1, the test tube 5 can be conveniently connected, cross contamination caused by manual excessive contact is avoided, after the connection is finished, the lead screw 8 is reversely rotated to drive the mounting seat 3 to descend, the test tube 5 is positioned in the box body 1, the box cover 2 is closed, the test tube 5 is sealed by the sealing plug 7, and the nucleic acid in the test tube 5 can be effectively prevented from being contaminated; the pull rod 15 connected with the clamping block 14 is pulled to separate the clamping block 14 from the clamping groove 11, so that the box cover 2 can be conveniently separated from the box body 1, the pull rod 15 is pulled to slide the clamping block 14 into the sliding groove 12 and compress the spring 13, the box cover 2 is arranged in the box body 1, when the clamping block 14 corresponds to the clamping groove 11 on the box cover 2, the pull rod 15 is loosened, the spring 13 is reset, the clamping block 14 can be clamped with the clamping groove 11, the installation of the box cover 2 is completed, and the disassembly and assembly of the box cover 2 are conveniently controlled.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (10)
1. The nucleic acid extraction kit capable of preventing cross contamination comprises a kit body (1) and is characterized in that the kit body (1) is of a hollow structure with an open top, the open position is connected with a matched box cover (2) through a clamping mechanism, a matched mounting seat (3) is arranged in the kit body (1), the upper surface of the mounting seat (3) is provided with a plurality of test tube grooves (4) which are uniformly distributed, matched test tubes (5) are inserted in the test tube grooves (4), an elastic clamp (6) is arranged between the test tubes (5) and the notches of the test tube grooves (4), the lower surface of the box cover (2) corresponding to the positions of the tube openings of the test tubes (5) is fixedly connected with matched sealing plugs (7), the sealing plugs (7) extend into the test tubes (5), a lead screw (8) is rotatably connected in the kit body (1) through a bearing, one end, far away from the bearing, of the lead screw (8) penetrates through the side wall of the kit body (1, the lead screw (8) is rotatably connected with the side wall of the box body (1), two opposite-direction threads are arranged on the lead screw (8), the lead screw (8) is arranged below the mounting seat (3), two matched moving blocks (9) are symmetrically in threaded connection on the lead screw (8), two connecting rods (10) are symmetrically hinged to the lower surface of the mounting seat (3), and the other ends of the connecting rods (10) are hinged to the upper surfaces of the corresponding moving blocks (9).
2. The nucleic acid extraction kit for preventing cross contamination according to claim 1, wherein the clamping mechanism comprises two clamping grooves (11) symmetrically formed at two ends of the box cover (2), a sliding groove (12) is formed in a position of the side wall of the box body (1) corresponding to the clamping groove (11), a spring (13) is fixedly connected to the bottom of the sliding groove (12), a clamping block (14) matched with the clamping groove (11) is fixedly connected to the other end of the spring (13), the clamping block (14) is slidably connected with the sliding groove (12), one end of the clamping block (14) far away from the spring (13) penetrates through the notch of the sliding groove (12) and extends into the clamping groove (11), a pull rod (15) is fixedly connected to one side of the clamping block (14) close to the bottom of the sliding groove (12), and the other end of the pull rod (15) penetrates through the spring (13) and extends outwards through the bottom of the sliding groove (12), and the pull rod (15) is connected with the bottom of the sliding groove (12) in a sliding way.
3. The nucleic acid extraction kit for preventing cross contamination according to claim 1, wherein the bottom of the mounting seat (3) is symmetrically and fixedly connected with an L-shaped rod (16), the other end of the L-shaped rod (16) is fixedly connected with a sliding block (17), a matching sliding groove (18) is formed in the inner wall of the box body (1) at a position corresponding to the sliding block (17), the sliding block (17) abuts against the side wall of the sliding groove (18) near the bottom end, and the sliding block (17) is slidably connected with the sliding groove (18).
4. The nucleic acid extraction kit for preventing cross contamination according to claim 1, wherein a moving rod (19) is fixedly connected to a lower surface of the moving block (9), a roller (20) is fixedly mounted at a bottom end of the moving rod (19), a roller groove (21) matched with the roller (20) is formed in a lower surface of the interior of the box body (1), and the roller (20) is in rolling connection with the roller groove (21).
5. The nucleic acid extraction kit for preventing cross contamination according to claim 1, wherein a rotating handle (22) is fixedly connected to one end of the screw rod (8) far away from the bearing.
6. The nucleic acid extraction kit for preventing cross contamination according to claim 1, wherein the opposite inner walls of the box body (1) near the top are symmetrically and fixedly connected with positioning blocks (23), and the lower surface of the box cover (2) is abutted against the upper surfaces of the positioning blocks (23).
7. The nucleic acid extraction kit for preventing cross contamination according to claim 1, wherein a handle (24) is fixedly connected to the upper surface of the box cover (2).
8. The nucleic acid extraction kit for preventing cross contamination according to claim 2, wherein a first magnetic block (25) is fixedly attached to the pull rod (15) located outside the sliding groove (12), a support rod (26) symmetrical with respect to the position of the pull rod (15) is fixedly attached to the outer side wall of the box body (1), a second magnetic block (27) is fixedly attached to the other end of the two support rods (26), the pull rod (15) penetrates through the second magnetic block (27), and the pull rod (15) is slidably connected with the second magnetic block (27).
9. The nucleic acid extraction kit for preventing cross contamination according to claim 2, wherein a pull ring (28) is fixedly connected to an end of the pull rod (15) away from the latch (14).
10. The nucleic acid extraction kit for preventing cross contamination according to claim 3, wherein a ball groove (29) is formed in one side of the slide block (17) close to the bottom of the slide groove (18), a ball (30) is arranged in the ball groove (29), and one end of the ball (30) far away from the bottom of the ball groove (29) penetrates through the notch of the ball groove (29) and extends outwards, and is connected to the bottom of the slide groove (18) in a rolling manner.
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CN201921376320.1U CN210656892U (en) | 2019-08-23 | 2019-08-23 | Nucleic acid extraction kit for preventing cross contamination |
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CN201921376320.1U CN210656892U (en) | 2019-08-23 | 2019-08-23 | Nucleic acid extraction kit for preventing cross contamination |
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CN114480071B (en) * | 2022-01-26 | 2024-01-30 | 深圳潘道医学检验实验室 | PCR nucleic acid extraction device convenient for operation of staff |
CN115639368A (en) * | 2022-10-25 | 2023-01-24 | 南京帝基生物科技有限公司 | Rapid detection kit for respiratory viruses and preparation method thereof |
CN115639368B (en) * | 2022-10-25 | 2024-04-26 | 南京帝基生物科技有限公司 | Rapid detection kit for respiratory viruses and preparation method thereof |
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