CN114480071A - PCR nucleic acid extraction device convenient to staff's operation - Google Patents
PCR nucleic acid extraction device convenient to staff's operation Download PDFInfo
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- CN114480071A CN114480071A CN202210096773.9A CN202210096773A CN114480071A CN 114480071 A CN114480071 A CN 114480071A CN 202210096773 A CN202210096773 A CN 202210096773A CN 114480071 A CN114480071 A CN 114480071A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 35
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 35
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 35
- 238000000605 extraction Methods 0.000 title claims abstract description 21
- 238000002474 experimental method Methods 0.000 claims abstract description 19
- 230000005540 biological transmission Effects 0.000 claims description 69
- 238000000429 assembly Methods 0.000 claims description 14
- 230000000712 assembly Effects 0.000 claims description 14
- 230000001105 regulatory effect Effects 0.000 claims description 14
- 230000008878 coupling Effects 0.000 claims description 9
- 238000010168 coupling process Methods 0.000 claims description 9
- 238000005859 coupling reaction Methods 0.000 claims description 9
- 230000001276 controlling effect Effects 0.000 claims description 6
- 238000006073 displacement reaction Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000001174 ascending effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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Abstract
The utility model relates to a PCR nucleic acid extraction element convenient to staff's operation, PCR nucleic acid extraction element includes the casing, the door body, the plummer, the telescopic link, first dwang and second dwang, the cavity of casing is the experiment cabin, the opening with experiment cabin intercommunication is seted up to casing one side, door body one side is rotated with the opening lateral wall and is connected, so that door body free end rotates to the direction of being close to or keeping away from ground, first dwang one end is rotated with the door body and is connected, the other end rotates with plummer one side and is connected, second dwang one end is rotated with the plummer opposite side and is connected, the other end rotates with experiment cabin lateral wall and is connected, telescopic link one end slides with the plummer and is connected, the other end and experiment cabin diapire fixed connection. When the staff opened the door body, the door body drove first dwang and rotates to make the second dwang rotate, simultaneously because the limiting displacement of telescopic link to the plummer, thereby make the plummer to removing near open-ended direction, and then realize the purpose of the staff's operation of being convenient for.
Description
Technical Field
The application relates to the field of equipment for PCR nucleic acid extraction and detection, in particular to a PCR nucleic acid extraction device convenient for workers to operate.
Background
The nucleic acid extraction device is an instrument which automatically finishes the work of extracting the nucleic acid of a sample by using a matched nucleic acid extraction reagent.
In the related art, for example, chinese patent with patent publication No. CN210711506U proposes a multi-sample general-purpose nucleic acid extraction instrument, which includes a nucleic acid extraction instrument body, a transparent sealing door, a magnetic frame, a magnetic rod sleeve frame, a magnetic rod sleeve, a fixing frame, a deep hole plate, a heating element, a first positioning element, and a second positioning element. The nucleic acid extractor body is provided with a touch control type operation panel, and an experiment cabin is arranged inside the touch control type operation panel; the transparent sealing door is arranged on the nucleic acid extractor body and seals the experiment chamber; the magnetic force frame is arranged on the nucleic acid extractor body and is positioned in the experiment cabin, the magnetic rod sleeve frame is arranged on the nucleic acid extractor body and is positioned in the experiment cabin, and the magnetic force frame is positioned above the magnetic rod sleeve frame; the magnetic bar is vertically arranged on the magnetic frame, and the magnetic bar sleeve is vertically arranged on the magnetic bar sleeve frame. The opening of the fixed frame faces upwards and is arranged on the nucleic acid extraction instrument body, and the fixed frame is positioned below the magnetic rod sleeve frame; in the deep hole board inserted fixed frame, the heating member setting can be contradicted on fixed frame and with the deep hole board, and first setting element transversely sets up on fixed frame and inserts the deep hole board, and the vertical setting of second setting element inserts the deep hole board in fixed frame. The staff passes through touch-control formula operating panel control extraction appearance, utilizes the bar magnet on the magnetic frame in the machine, and the magnetic bead that will adsorb the nucleic acid moves to different reagent inslot, utilizes the bar magnet cover of cover in the bar magnet outer layer to stir liquid again, thereby makes it mix uniformly and realize the extraction of nucleic acid.
With respect to the related art among the above, the inventors consider that the following drawbacks exist: because the space is less in the experiment cabin, it is comparatively inconvenient that the staff gets at every turn and puts the deep hole board.
Disclosure of Invention
In order to realize the purpose of facilitating the operation of workers, the application provides a PCR nucleic acid extraction device facilitating the operation of the workers.
The application provides a PCR nucleic acid extraction device convenient to staff's operation relates to following technical scheme:
the utility model provides a PCR nucleic acid extraction element convenient to staff's operation, includes the casing and the door body, and the cavity of casing is the experiment cabin, the opening with experiment cabin intercommunication is seted up to casing one side, door body one side is rotated with the opening lateral wall and is connected to make a body free end to the direction of being close to or keeping away from ground rotates, PCR nucleic acid extraction element still includes plummer, telescopic link, first dwang and second dwang, first dwang one end is rotated with the door body and is connected, and the other end rotates with plummer one side and is connected, second dwang one end is rotated with the plummer opposite side and is connected, and the other end rotates with experiment cabin lateral wall and is connected, telescopic link one end slides with the plummer and is connected, other end and experiment cabin diapire fixed connection.
Through adopting above-mentioned technical scheme, when the staff opened the door body, the door body drove first dwang and rotates to make the second dwang rotate, simultaneously because the limiting displacement of telescopic link to the plummer, thereby make the plummer to removing near the open-ended direction, and then realize the purpose of the staff's operation of being convenient for.
Optionally, be provided with the fixed subassembly that is used for fixed deep hole board on the plummer, fixed subassembly includes polylith fixed plate, polylith the fixed plate sets up along deep hole board circumference, and the fixed plate passes through coupling assembling and is connected with the plummer to make the fixed plate to the direction removal that is close to or keeps away from the deep hole board.
Through adopting above-mentioned technical scheme, place the deep hole board on the plummer, control coupling assembling to make coupling assembling drive the fixed plate and remove, conflict until fixed plate and deep hole board, thereby accomplish the fixed of deep hole board, with the difficult mesh that rocks of deep hole board in the realization PCR nucleic acid extraction element operation process.
Optionally, the fixed plate is contradicted with the plummer mesa, coupling assembling includes connecting rod and connecting sleeve, connecting sleeve and fixed plate fixed connection, the connecting rod rotate with the plummer be connected and with connecting sleeve threaded connection, be provided with on the plummer and be used for driving connecting rod pivoted actuating mechanism.
Through adopting above-mentioned technical scheme, control actuating mechanism to make actuating mechanism drive the connecting rod and rotate, because connecting rod and connecting sleeve threaded connection, the connecting sleeve is difficult for rotating along with the connecting rod under the fixed plate effect, thereby makes the connecting sleeve drive the fixed plate under the connecting rod effect and removes to the direction of being close to or keeping away from the deep hole board.
Optionally, the driving mechanism includes a driving assembly and a plurality of transmission assemblies, the plurality of transmission assemblies correspond to the plurality of fixing plates one to one, and the plurality of transmission assemblies are all connected with the driving assembly, the driving assembly includes a driving bevel gear, a driving rack and a driving gear, the driving rack is fixedly connected with one end of the telescopic rod far away from the inner wall of the shell, the driving gear is rotatably connected with the bearing platform and meshed with the driving rack, and the driving bevel gear is coaxially and fixedly connected with the driving gear and connected with the transmission assemblies.
Through adopting above-mentioned technical scheme, when the staff opened the door body, the door body drove first pivot pole to make first pivot pole drive the plummer and remove to being close to the open-ended direction, thereby make drive gear and drive rack emergence relative displacement, and then make drive gear rotate, and drive bevel gear and make transmission assembly drive the connecting rod and rotate, in order to realize the fixed plate to being close to or keeping away from the purpose of the removal of deep hole board direction.
Optionally, the transmission assembly comprises a first transmission bevel gear, a second transmission bevel gear, a third transmission bevel gear, a fourth transmission bevel gear and a fifth transmission bevel gear, the first transmission bevel gear is coaxially and fixedly connected with the connecting rod, the second transmission bevel gear is coaxially and fixedly connected with the third transmission bevel gear, the first transmission bevel gear and the third transmission bevel gear are both rotatably connected with the bearing table, the second transmission bevel gear is meshed with the first transmission bevel gear, the fourth transmission bevel gear is coaxially and fixedly connected with the fifth transmission bevel gear, the fourth transmission bevel gear and the fifth transmission bevel gear are both rotatably connected with the bearing table, the fourth transmission bevel gear is meshed with the third transmission bevel gear, and the fifth transmission bevel gear is meshed with the driving bevel gear.
By adopting the technical scheme, the driving bevel gear rotates to drive the fifth transmission bevel gear to rotate so as to drive the fourth transmission bevel gear to drive the third transmission bevel gear to rotate, and further the second transmission bevel gear drives the first transmission bevel gear and enables the connecting rod to rotate.
Optionally, one side of the fixing plate close to the deep hole plate is fixedly connected with an elastic pad.
Through adopting above-mentioned technical scheme, the setting of cushion has improved the closely knit nature of contradicting between fixed plate and the deep hole board.
Optionally, a sliding groove along the radial direction of the connecting rod is formed in the connecting rod, positioning grooves corresponding to the sliding grooves one to one are formed in the first transmission bevel gear, sliding blocks connected with the side walls of the sliding grooves in a sliding mode are arranged in the sliding grooves, and adjusting assemblies used for adjusting the sliding of the sliding blocks are arranged on the connecting rod.
Through adopting above-mentioned technical scheme, control adjusting part to make the sliding block part slide in the constant head tank, thereby first transmission bevel gear is difficult for continuing to drive the connecting rod and rotates, thereby makes the conflict power between fixed plate and the deep hole difficult too big.
Optionally, the adjusting part is including adjusting pole and regulating spring, set up on the connecting rod with the holding chamber of the groove intercommunication that slides, adjust the pole and slide along connecting rod length direction and holding chamber lateral wall and be connected, along the direction that is close to the fixed plate, adjust the pole and reduce along the ascending size in sliding block sliding direction gradually, regulating spring one end and sliding groove lateral wall fixed connection, the other end and sliding block fixed connection, under the effect of regulating spring elasticity, the sliding block is contradicted with the regulation pole, be provided with the regulation and control subassembly that is used for regulating and control to adjust the pole and slides on the connecting rod, the side slope that the sliding block is close to the constant head tank bottom sets up.
Through adopting above-mentioned technical scheme, control regulation and control subassembly to make the regulation pole remove and drive the sliding block and slide along the sliding groove, thereby realize the regulation of sliding block and constant head tank joint state.
In summary, the present application includes at least one of the following advantageous technical effects:
1. the arrangement of the first rotating rod, the second rotating rod and the bearing table realizes the purpose of facilitating the operation of workers;
2. the arrangement of the fixing plate realizes the purpose that the deep hole plate is not easy to shake.
Drawings
FIG. 1 is a schematic diagram of the overall structure of an embodiment of the present application;
FIG. 2 is a partial cross-sectional view of an embodiment of the present application;
FIG. 3 is an enlarged schematic view of portion A of FIG. 2;
FIG. 4 is a partial cross-sectional view of an embodiment of the present application to illustrate an adjustment assembly;
fig. 5 is an enlarged schematic view of a portion B in fig. 4.
Description of reference numerals: 100. a housing; 110. a bearing table; 111. a chute; 112. an accommodating chamber; 120. a telescopic rod; 121. a support sub-rod; 122. a connecting sub-rod; 123. a slider; 130. a first rotating lever; 140. a second rotating lever; 150. an opening; 200. a door body; 300. a fixing plate; 311. connecting holes; 400. a connecting assembly; 410. a connecting rod; 411. a sliding groove; 412. a sliding block; 413. an accommodating cavity; 420. a connecting sleeve; 500. a drive mechanism; 600. a drive assembly; 610. a drive bevel gear; 620. a drive gear; 630. a drive rack; 700. a transmission assembly; 710. a first drive bevel gear; 711. positioning a groove; 720. a second drive bevel gear; 730. a third drive bevel gear; 740. a fourth drive bevel gear; 750. a fifth drive bevel gear; 760. a connecting rod; 800. an adjustment assembly; 810. adjusting the spring; 820. adjusting a rod; 900. a regulatory component; 910. a regulating and controlling rod; 920. regulating and controlling a spring; 930. a regulatory block.
Detailed Description
The present application is described in further detail below with reference to figures 1-5.
The embodiment of the application discloses a PCR nucleic acid extraction device convenient for workers to operate. Referring to FIG. 1, the PCR nucleic acid extracting apparatus includes a housing 100, a cavity of the housing 100 is a test chamber, and an opening 150 is formed on a side wall of the housing 100 and is communicated with the test chamber. The side wall of the casing 100 is rotatably connected with a door 200 for opening and closing the opening 150, and the position where the door 200 is rotatably connected with the casing 100 is located on one side of the opening 150 close to the ground. The casing 100 and the door body 200 are both fixedly connected with magnetic sheets, and the casing 100 is magnetically connected with the magnetic sheets on the door body 200.
Referring to FIG. 2, the PCR nucleic acid isolation apparatus further includes a platform 110, a retractable rod 120, a first rotating rod 130 and a second rotating rod 140 disposed in the cavity of the housing 100, in this embodiment, two first rotating rods 130 and two second rotating rods 140 are disposed, and the first rotating rod 130 and the second rotating rod 140 are separately disposed at two sides of the platform 110. Two first rotating rods 130 are arranged in parallel at intervals, one end of each first rotating rod 130 is rotatably connected with the door body 200, and the other end of each first rotating rod 130 is rotatably connected with the side wall of the corresponding side of the bearing table 110. The two second rotating rods 140 are arranged in parallel at intervals, one end of each second rotating rod 140 is rotatably connected with the corresponding side wall of the plummer 110, and the other end of each second rotating rod 140 is rotatably connected with the side wall of the experiment chamber.
The telescopic rod 120 comprises a supporting sub-rod 121 and a connecting sub-rod 122, one end of the supporting sub-rod 121 is fixedly connected with the bottom wall of the experiment chamber, the other end of the supporting sub-rod is slidably sleeved on the outer side of the connecting sub-rod 122, and one end, far away from the supporting sub-rod 121, of the connecting sub-rod 122 is slidably connected with the bearing table 110. In this embodiment, the plummer 110 is provided with a sliding groove 111 along a direction close to or far away from the opening 150, the connecting rod 122 is fixedly connected with a sliding block 123, and the sliding block 123 slides along the length direction of the sliding groove 111 and is connected with the side wall of the sliding groove 111, so that the sliding connection between the telescopic rod 120 and the plummer 110 is realized.
In order to make the staff place the back on plummer 110 with the deep hole board, the difficult emergence of deep hole board is removed, is provided with the fixed subassembly that is used for fixed deep hole board on the plummer 110 consequently.
Referring to fig. 2 and 3, the cross-section of the deep hole plate is quadrilateral, the fixing assembly includes four fixing plates 300, the four fixing plates 300 are circumferentially arranged along the deep hole plate, each fixing plate 300 corresponds to a side wall of the deep hole plate, and the fixing plate 300 is connected with the bearing table 110 through the connecting assembly 400, so that the fixing plate 300 is moved along a direction close to or away from the deep hole plate and is abutted against the corresponding side wall of the deep hole plate.
Coupling assembling 400 includes connecting rod 410 and connecting sleeve 420, fixedly connected with engaging lug on the plummer 110, connecting rod 410 uses its self axis to rotate for axis of rotation and engaging lug and be connected, connecting sleeve 420 one end and fixed plate 300 fixed connection, the other end and connecting rod 410 threaded connection, fixed plate 300 is contradicted with plummer 110 mesa, thereby when making connecting rod 410 rotate, connecting sleeve 420 and connecting rod 410 can rotate relatively, so that connecting sleeve 420 drives fixed plate 300 and removes to the direction that is close to or keeps away from the deep hole board.
The susceptor 110 has a receiving cavity 112. The carrier table 110 is provided with a driving mechanism 500 for driving the plurality of connecting rods 410 to rotate synchronously. The driving mechanism 500 includes four sets of transmission assemblies 700 and driving assemblies 600, wherein the transmission assemblies 700 are disposed in four sets, and one set of transmission assembly 700 is disposed corresponding to one connecting rod 410. The present embodiment is illustrated with a set of transmission assemblies 700 as an example. The driving assembly 700 includes a first driving bevel gear 710, a second driving bevel gear 720, a third driving bevel gear 730, a fourth driving bevel gear 740, a fifth driving bevel gear 750, and an engaging rod 760. The first transmission bevel gear 710 is coaxially connected with the connecting rod 410 and is engaged with the second transmission bevel gear 720. The second transmission bevel gear 720 and the third transmission bevel gear 730 are coaxially and fixedly connected through a rotating shaft, and the rotating shaft is rotatably connected with the bearing table 110. The fourth bevel drive gear 740 and the fifth bevel drive gear 750 are coaxially and fixedly connected by an engaging rod 760, and the engaging rod 760 is rotatably connected to the side wall of the accommodating chamber 112. The fourth bevel drive gear 740 is engaged with the third bevel drive gear 730, and the fifth bevel drive gear 750 is connected to the driving assembly to drive the fifth bevel drive gear to rotate.
The driving assembly 600 is controlled to rotate the fifth bevel gears 750 of the four sets of driving assemblies 700, that is, the engaging rods 760 drive the fourth bevel gears 740 to rotate, so that the third bevel gears 730 drive the rotating shafts to rotate the second bevel gears 720, and the second bevel gears 720 are engaged with the first bevel gears 710 to rotate the first bevel gears 710 and drive the connecting rods 410 to rotate.
The driving assembly 600 includes a driving bevel gear 610, a driving gear 620 and a driving rack 630, wherein the driving rack 630 is disposed along the sliding direction of the sliding block 123 and is fixedly connected with the sliding block 123. The driving gear 620 is disposed in the sliding slot 111 and is rotatably connected to a sidewall of the sliding slot 111, and the driving gear 620 is engaged with the driving rack 630 and is coaxially and fixedly connected to the driving bevel gear 610.
When the worker opens the door body 200, the door body 200 drives the first rotating rod 130 to rotate, so that the bearing platform 110 moves towards the direction close to the ground, and moves relative to the sliding block 123, that is, the driving gear 620 and the driving rack 630 move relative to each other, so that the driving gear 620 rotates, and the driving bevel gear 610 is driven to rotate, so that the transmission assembly 700 drives the corresponding connecting rod 410 to rotate.
Referring to fig. 4 and 5, in order to adapt the fixing assembly to the positioning of deep hole plates with different sizes, a plurality of sliding grooves 411 are formed in the connecting rod 410 along the radial direction of the connecting rod 410, sliding blocks 412 connected with the side walls of the sliding grooves 411 in a sliding manner are arranged in the sliding grooves 411, and one side surfaces of the sliding blocks 412 are inclined surfaces. The first transmission bevel gear 710 is provided with positioning grooves 711 corresponding to the sliding grooves 411 one by one, the positioning grooves 711 are opposite to the notches of the sliding grooves 411, and the sliding block 412 and the positioning grooves 711 can be clamped.
The connecting rod 410 is provided with an adjusting component 800 for adjusting the sliding of the sliding block 412. The adjusting assembly 800 comprises an adjusting spring 810 and an adjusting rod 820, the adjusting spring 810 is located in the sliding slot 411, one end of the adjusting spring 810 is fixedly connected with the sliding block 412, and the other end of the adjusting spring 810 is fixedly connected with the side wall of the sliding slot 411. The connecting rod 410 is internally provided with an accommodating cavity 413 communicated with the sliding groove 411, the adjusting rod 820 is connected with the side wall of the accommodating cavity 413 in a sliding manner along the axial direction of the connecting rod 410, the side wall of the sliding block 412 far away from the positioning groove 711 can abut against the adjusting rod 820, and the diameter of the adjusting rod 820 is gradually reduced along the direction close to the fixing plate 300. The connecting rod 410 is provided with a regulating component 900 for regulating and controlling the sliding of the regulating rod 820.
The fixing plate 300 is formed with a connection hole 311 communicating with the accommodating chamber 413. The adjustment assembly 900 includes an adjustment lever 910, an adjustment spring 920, and an adjustment block 930. The control block 930 is slidably coupled to a sidewall of the coupling hole 311 along a length direction of the coupling hole 311. One end of the adjusting and controlling rod 910 is fixedly connected to the adjusting and controlling block 930, and the other end thereof penetrates into the accommodating cavity 413 and is rotatably connected to the adjusting rod 820. One end of the adjusting spring 920 is fixedly connected with the adjusting rod 910, and the other end is fixedly connected with the inner wall of the connecting sleeve 420, and the adjusting block 930 partially extends out of the connecting hole 311 under the elastic force of the adjusting spring 920.
When the adjusting block 930 abuts against the deep hole plate, the fixing plate 300 continues to move in a direction close to the deep hole plate, the adjusting rod 910 drives the adjusting rod 820 and the connecting rod 410 to move relatively, when the fixing plate 300 moves to abut against the side wall of the deep hole plate, the adjusting rod 910 drives the adjusting rod 820 to move to a position where the abutting state of the adjusting rod 820 is released, at this time, the sliding block 412 slides under the elastic force of the adjusting spring 810 to a position where the inclined surface of the sliding block 412 abuts against the side wall of the positioning groove 711, so that when the first transmission bevel gear 710 continues to rotate, the side wall of the positioning groove 711 pushes the sliding block 412 to move in a direction close to the adjusting rod 820, so that the first transmission bevel gear 710 and the connecting rod 410 rotate relatively, and the fixing plate 300 cannot continue to move in a direction close to the deep hole plate; when the first transmission bevel gear 710 reversely rotates, so that the fixing plate 300 moves towards the direction far away from the deep hole plate, the side surface of the sliding block 412 is abutted against the side wall of the positioning groove 711, the first transmission bevel gear 710 and the connecting rod 410 are not easy to rotate relatively, and the first transmission bevel gear 710 rotates to drive the connecting rod 410 to rotate.
The implementation principle of the embodiment of the application is as follows: the installation of the deep hole plate can be achieved by placing the deep hole plate on the bearing table 110 and then rotating the door body 200 to close the opening 150.
The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.
Claims (8)
1. The utility model provides a PCR nucleic acid extraction element convenient to staff's operation, includes casing (100) and door body (200), and the cavity of casing (100) is the experiment cabin, opening (150) with the experiment cabin intercommunication are seted up to casing one side, door body (200) one side is rotated with opening (150) lateral wall and is connected to make door body (200) free end to the direction rotation that is close to or keeps away from ground, its characterized in that: PCR nucleic acid extraction element still includes plummer (110), telescopic link (120), first dwang (130) and second dwang (140), first dwang (130) one end is rotated with the door body (200) and is connected, and the other end rotates with plummer (110) one side and is connected, second dwang (140) one end rotates with plummer (110) opposite side to be connected, and the other end rotates with experiment cabin lateral wall to be connected, telescopic link (120) one end slides with plummer (110) and is connected, the other end and experiment cabin diapire fixed connection.
2. The PCR nucleic acid extracting apparatus convenient for a worker to operate according to claim 1, wherein: be provided with the fixed subassembly that is used for fixed deep hole board on plummer (110), fixed subassembly includes polylith fixed plate (300), polylith fixed plate (300) set up along deep hole board circumference, and fixed plate (300) are connected with plummer (110) through coupling assembling (400) to make fixed plate (300) to the direction removal that is close to or keeps away from the deep hole board.
3. The PCR nucleic acid extracting apparatus convenient for a worker to operate according to claim 2, wherein: fixed plate (300) are contradicted with plummer (110) mesa, coupling assembling (400) are including connecting rod (410) and connecting sleeve (420), connecting sleeve (420) and fixed plate (300) fixed connection, connecting rod (410) rotate with plummer (110) be connected and with connecting sleeve (420) threaded connection, be provided with on plummer (110) and be used for driving connecting rod (410) pivoted actuating mechanism (500).
4. The PCR nucleic acid extracting apparatus convenient for a worker to operate according to claim 3, wherein: the driving mechanism (500) comprises a driving assembly (600) and a plurality of transmission assemblies (700), the transmission assemblies (700) correspond to the fixing plates (300) one by one, the transmission assemblies (700) are connected with the driving assembly (600), the driving assembly (600) comprises a driving bevel gear (610), a driving rack (630) and a driving gear (620), the driving rack (630) is fixedly connected with one end, far away from the inner wall of the shell (100), of the telescopic rod (120), the driving gear (620) is rotatably connected with the bearing table (110) and meshed with the driving rack (630), and the driving bevel gear (610) is coaxially and fixedly connected with the driving gear (620) and connected with the transmission assemblies (700).
5. The PCR nucleic acid extracting apparatus convenient for a worker to operate according to claim 4, wherein: the transmission assembly (700) comprises a first transmission bevel gear (710), a second transmission bevel gear (720), a third transmission bevel gear (730), a fourth transmission bevel gear (740) and a fifth transmission bevel gear (750), the first transmission bevel gear (710) is coaxially and fixedly connected with the connecting rod (410), the second transmission bevel gear (720) is coaxially and fixedly connected with the third transmission bevel gear (730), and both are rotationally connected with the bearing platform (110), the second transmission bevel gear (720) is meshed with the first transmission bevel gear (710), the fourth transmission bevel gear (740) is coaxially and fixedly connected with the fifth transmission bevel gear (750), and the fourth transmission bevel gear (740) is meshed with the third transmission bevel gear (730), and the fifth transmission bevel gear (750) is meshed with the driving bevel gear (610).
6. The PCR nucleic acid extracting apparatus convenient for a worker to operate according to claim 3, wherein: one side of the fixing plate (300) close to the deep hole plate is fixedly connected with an elastic pad.
7. The PCR nucleic acid extracting apparatus convenient for the worker to operate according to claim 5, wherein: offer on connecting rod (410) along radial sliding groove (411) of connecting rod (410), offer on first transmission bevel gear (710) with sliding groove (411) one-to-one constant head tank (711), be provided with in sliding groove (411) and slide sliding block (412) of being connected with sliding groove (411) lateral wall slip, but slide sliding block (412) and constant head tank (711) joint, be provided with on connecting rod (410) and be used for adjusting part (800) that slide sliding block (412) slided.
8. The PCR nucleic acid extracting apparatus convenient for a worker to operate according to claim 7, wherein: the adjustment assembly (800) comprises an adjustment lever (820) and an adjustment spring (810), the connecting rod (410) is provided with an accommodating cavity (413) communicated with the sliding groove (411), the adjusting rod (820) is connected with the side wall of the containing cavity (413) in a sliding way along the length direction of the connecting rod (410) and is close to the direction of the fixing plate (300), the size of the adjusting rod (820) along the sliding direction of the sliding block (412) is gradually reduced, one end of the adjusting spring (810) is fixedly connected with the side wall of the sliding groove (411), the other end is fixedly connected with the sliding block (412), under the elastic force of the adjusting spring (810), the sliding block (412) is pressed against the adjusting rod (820), the connecting rod (410) is provided with a regulating component for regulating and controlling the sliding of the regulating rod (820), the sliding block (412) is obliquely arranged close to the side surface of the bottom of the positioning groove (711).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210096773.9A CN114480071B (en) | 2022-01-26 | 2022-01-26 | PCR nucleic acid extraction device convenient for operation of staff |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210096773.9A CN114480071B (en) | 2022-01-26 | 2022-01-26 | PCR nucleic acid extraction device convenient for operation of staff |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114480071A true CN114480071A (en) | 2022-05-13 |
| CN114480071B CN114480071B (en) | 2024-01-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210096773.9A Active CN114480071B (en) | 2022-01-26 | 2022-01-26 | PCR nucleic acid extraction device convenient for operation of staff |
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| Country | Link |
|---|---|
| CN (1) | CN114480071B (en) |
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| CN114480071B (en) | 2024-01-30 |
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