CN115197812A

CN115197812A - Microbial culture device with high sealing performance and culture method thereof

Info

Publication number: CN115197812A
Application number: CN202210971797.4A
Authority: CN
Inventors: 于秋月
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-08-12
Filing date: 2022-08-12
Publication date: 2022-10-18

Abstract

The invention discloses a microbial cultivation device with high sealing performance and a cultivation method thereof, and relates to the field of microbial cultivation device structures. According to the invention, the culture dish to be taken out can be ejected through the groove support block through the mechanism, the baffle plate is shifted and the notch is exposed while the culture dish is ejected, so that the culture dish is ejected smoothly, external air is prevented from entering the box body to the maximum extent, the sealing property is maintained, when the telescopic rod retracts, the baffle plate is rapidly reset to plug the notch, the box body is kept in a sealing state, and the influence of the environment in the box in the external environment is reduced.

Description

Microbial culture device with high sealing performance and culture method thereof

Technical Field

The invention relates to the field of microbial culture device structures, in particular to a microbial culture device with high sealing performance and a culture method thereof.

Background

Microbial culture, which refers to the rapid growth and propagation of some (species) of microorganisms by means of artificially prepared culture media and artificially created culture conditions (such as culture temperature, etc.), is called microbial culture, which can be divided into pure culture and mixed culture, wherein the former refers to the culture and utilization of purified single strains; the latter means that microorganisms in mixed strains or natural samples (such as soil) are cultured, and then the diversity and the quantity of the microorganisms in the soil can be estimated to a certain extent according to the types and the quantity of the microorganisms growing on the culture medium, and the microorganism culture is generally carried out in a specific sealed incubator.

In the prior art, in the microbial process, a specific substance is generally added into a culture dish regularly, or the elongation condition of the microorganism is observed regularly, so that the culture dish needs to be taken out of a sealed incubator, the microorganism is contacted with the external environment, the growth environment of the microorganism is changed, the growth of the organism is influenced, and the use is inconvenient, so that the microbial culture device with strong sealing property and the culture method thereof are disclosed to meet the requirements of people.

Disclosure of Invention

The utility model aims to provide a microorganism culture apparatus and cultivation method that leakproofness is strong to in the microorganism process who proposes in solving above-mentioned background art, generally can add specific material to the culture dish in regular time, perhaps observe the microorganism extension condition regularly, need take out sealed incubator with the culture dish like this, cause microorganism and external environment to contact, changed the growth environment of microorganism, influence the growth of thing biology, use many inconvenient problems.

In order to achieve the above purpose, the present application provides the following technical solutions: the utility model provides a microorganism culture apparatus that leakproofness is strong, includes the box, the top side of box is equipped with sunken platform, the top surface of sunken platform is seted up jaggedly, the bottom side slidable mounting of sunken platform have with breach assorted baffle, the upper cover plate is installed to the top of sunken platform, install the stationary cylinder in the box, it rotates through the damping ring and installs downside ware frame and upside ware frame to go up the stationary cylinder, downside ware frame with all evenly seted up a plurality of step breach grooves on the ware frame of upside, it is a plurality of the culture dish has all been placed on the step groove, breach on the sunken platform with the culture dish phase-match, one side of downside ware frame has been seted up dodges the breach, downside ware frame with one side of upside ware frame is installed and is selected separately actuating mechanism, install first slide rail on the bottom side inner wall of box, slidable mounting has first slider on the first slide rail, the top side of first slider is installed the telescopic link, the drive unit is installed to one side of first slider, the top of telescopic link the recess tray, the top side of recess tray with the top side of culture dish, the bottom side assorted in the fastening piece install the recess, the synchronous opening unit with the baffle.

Preferably, select separately actuating mechanism includes initiative bevel gear, the slip unit is installed to one side of box, rotate through the pivot cover on the slip unit and install the dwang, initiative bevel gear cup joints and installs the one end of dwang, the other end extend to the outside of box is installed the second rolling disc, the top side of downside vessel frame with driven bevel gear is all installed to the bottom side of upside vessel frame, initiative bevel gear and two driven bevel gear meshes the matching in turn.

Preferably, the sliding unit includes a sliding plate, one side of the box body is provided with a mounting hole and is provided with a sliding chute frame, the sliding plate is slidably mounted on the sliding chute frame, the rotating shaft sleeve is mounted on the sliding plate, one side of the rotating shaft sleeve is provided with a transverse push rod, one side of the transverse push rod is provided with a fixing unit, and one side of the sliding chute frame is provided with a sealing unit.

Preferably, the fixing unit includes a bolt, a sliding hole is formed in one side of the transverse push rod, the bolt is slidably mounted in the sliding hole, two limiting slots are correspondingly mounted on one side of the box body, one end of the bolt is matched with the corresponding limiting slot, and the two limiting slots correspond to the two driven bevel gears.

Preferably, the sealing unit comprises an elastic sealing film, the elastic sealing film is sleeved on the rotating shaft sleeve, and the peripheral side of the elastic sealing film is arranged on one side of the chute frame.

Preferably, the driving unit comprises a threaded rod, a threaded hole is formed in one side of the first sliding block, a fixing frame is installed on the inner wall of the bottom side of the box body, the threaded rod is rotatably installed on the fixing frame, the threaded rod is matched with the threaded hole, and one end of the threaded rod extends to the outer side of the box body and is sleeved with a first rotating disc.

Preferably, the unit of opening in step includes the wedge, the second slide rail is installed to the bottom side of sunken platform, the baffle passes through the second slider and installs on the second slide rail, install one side of wedge one side of baffle, the gyro wheel is installed through the support rotation in one side of recess tray, the gyro wheel with the inclined plane phase-match of wedge, the unit that resets is installed to one side of wedge.

Preferably, the reset unit comprises a spring, one end of the spring is installed on one side of the wedge-shaped block, and the other end of the spring is installed on the inner side wall of the box body.

Preferably, the auxiliary fastening unit is provided with a sucker in a groove of the groove support block, and the top side of the groove support block and the sucker are both provided with the sucker.

Preferably, the method for culturing the microbial culture device with strong sealing performance comprises the following steps:

s1, during normal culture, the box body is sealed by an upper cover plate, a specific environment is arranged in the box body, a culture medium is placed in the culture dish and microorganisms are cultured, and the culture dishes can be loaded by the step gap grooves on the lower side dish frame and the upper side dish frame to simultaneously culture the microorganisms;

s2, when microorganisms need to be checked or observed, the upper cover plate is opened, the lower side vessel frame or the upper side vessel frame is selectively driven to rotate through the sorting driving mechanism, and the culture vessel needing to be taken out is rotated to a position corresponding to the notch on the sunken platform;

s3, moving a telescopic rod through a driving unit to enable a groove support block to correspond to the culture dish to be taken out, enabling the groove support block to support the bottom side of the culture dish through extension of the telescopic rod, fixing the culture dish through an auxiliary fastening unit, enabling the telescopic rod to extend continuously and enabling the culture dish to be separated from a step gap groove, enabling the culture dish to correspond to a gap on the concave platform through the first sliding block, enabling the telescopic rod to extend continuously and enabling the culture dish to be ejected out through the gap, and waiting for next operation;

s4, the avoidance notch can avoid the telescopic rod, the groove supporting block is ejected out to be located on the upper side vessel frame, the baffle is matched with the notch on the sunken platform and seals the notch, the box body is kept in a sealing state, when the telescopic rod is continuously extended to enable the culture dish to be ejected out through the notch, the synchronous opening unit enables the baffle to be opened and the notch to be exposed, the culture dish is smoothly ejected out, external air is prevented from entering the box body to the maximum extent, the sealing performance is kept, and when the telescopic rod retracts, the baffle is rapidly reset to seal the notch, so that the box body is kept in a sealing state.

In conclusion, the technical effects and advantages of the invention are as follows:

1. according to the invention, a specific environment is set in the box body, a culture medium is placed in the culture dish and microorganisms are cultured, when the microorganisms need to be checked or observed, the culture dish which needs to be taken out can be ejected through the groove support block through the mechanism, the baffle plate is shifted and the gap is exposed while the culture dish is ejected, so that the culture dish is smoothly ejected, external air is prevented from entering the box body to the maximum extent, the sealing property is kept, when the telescopic rod retracts, the baffle plate is quickly reset to plug the gap, the box body is kept in a sealing state, and the influence of the environment in the box body in the external environment is reduced.

2. According to the culture dish taking and placing device, the first rotating disc is rotated to enable the rotating rod to rotate on the rotating shaft sleeve through the arrangement of the driving bevel gear, the transverse push rod is pushed to enable the sliding plate to slide on the sliding groove frame through the arrangement of the sliding plate, so that the position of the rotating rod is changed, after the rotating rod slides to a corresponding position, the rotating shaft sleeve and the rotating rod can synchronously move along with the sliding plate through the locking position of the fixing unit, the driving bevel gear is meshed with one driven bevel gear, the corresponding lower side dish rack or the corresponding upper side dish rack rotates, and the purpose that a single culture dish can be taken and placed at will is achieved.

3. According to the invention, the elastic sealing film has certain elasticity and deforms when the rotating rod moves, so that the elastic sealing film can always cover one side of the sliding groove frame, the sealing of the sliding groove frame is kept, the sealing performance of the box body is kept, and through the arrangement of the wedge-shaped block, in the extension process of the telescopic rod, the roller is in contact with the inclined surface of the wedge-shaped block and pushes the wedge-shaped block, so that the wedge-shaped block slides to one side far away from the telescopic rod along the second sliding rail and drives the baffle to slide together, the gap is exposed, the probability of communication between the interior and the exterior of the box body is reduced, and the sealing performance is improved.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic view of a cutaway structure of the present invention;

FIG. 3 is a partial perspective view of a stationary cylindrical region according to the present invention;

FIG. 4 is a schematic view of a partially enlarged structure of a rotating rod region in the present invention;

FIG. 5 is a schematic view of the slide plate of the present invention in a partially cut-away configuration;

fig. 6 is a partial sectional structure diagram of the wedge block area in the invention.

In the figure: 1. a box body; 2. a recessed platform; 3. a piston cylinder; 4. a second rotating disk; 5. an elastic sealing film; 6. a first rotating disk; 7. a chute frame; 8. a transverse push rod; 9. fixing the cylinder; 10. a lower-side vessel holder; 11. an upper side vessel rack; 12. a culture dish; 13. a driven bevel gear; 14. rotating the rod; 15. a telescopic rod; 16. an air tube; 17. a first slider; 18. a first slide rail; 19. a threaded rod; 20. a step notch groove; 21. avoiding the notch; 22. a damping ring; 23. a drive bevel gear; 24. a sliding plate; 25. a groove support block; 26. a suction cup; 27. a support; 28. a roller; 29. a baffle plate; 30. a wedge block; 31. a spring; 32. a second slide rail; 33. a second slider; 34. a rotating shaft sleeve; 35. limiting slots; 36. a bolt; 37. and an upper cover plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example (b): referring to fig. 1-6, a microorganism culture apparatus with strong sealing performance, including a box 1, a concave platform 2 is arranged on the top side of the box 1, a notch is formed on the top surface of the concave platform 2, a baffle 29 matched with the notch is slidably mounted on the bottom side of the concave platform 2, an upper cover plate 37 is mounted above the concave platform 2, a fixed cylinder 9 is mounted in the box 1, a lower-side vessel frame 10 and an upper-side vessel frame 11 are rotatably mounted on the fixed cylinder 9 through a damping ring 22, a plurality of step notch grooves 20 are uniformly formed on the lower-side vessel frame 10 and the upper-side vessel frame 11, culture vessels 12 are respectively placed on the step notch grooves 20, notches on the concave platform 2 are matched with the culture vessels 12, an avoiding notch 21 is formed on one side of the lower-side vessel frame 10, a sorting driving mechanism is mounted on one side of the lower-side vessel frame 10 and the upper-side vessel frame 11, a first slide rail 18 is mounted on the inner wall of the bottom side of the box 1, a first slide rail 18 is slidably mounted with a first slide block 17, a telescopic rod 15 is mounted on the top side of the first slide rail 17, a fastening block 25 is mounted on the top side of the telescopic rod 15, a groove 25 matched with a groove 25, a synchronous opening unit of a groove 25 mounted in the synchronous opening assisting block 25 mounted on the groove 25 of the groove 25, and a synchronous opening unit of the synchronous groove 25 mounted in the synchronous groove 25.

Based on the above mechanism, the box body 1 is a transparent sealed box body, during normal culture, the upper cover plate 37 seals the box body 1, the interior of the box body 1 is arranged in a specific environment, culture mediums are placed in the culture dishes 12 and microorganisms are cultured, the plurality of stepped notch grooves 20 on the lower side dish rack 10 and the upper side dish rack 11 can bear a plurality of culture dishes 12 for simultaneous microorganism culture, when the microorganisms need to be checked or observed, the upper cover plate 37 is opened, the sorting driving mechanism selectively drives the lower side vessel frame 10 or the upper side vessel frame 11 to rotate, and the culture vessel 12 to be taken out is rotated to the position corresponding to the notch on the sunken platform 2, the telescopic rod 15 is moved by the driving unit so that the groove holder 25 corresponds to the culture dish 12 to be taken out, and the groove holder 25 is held on the bottom side of the culture dish 12 by extending the telescopic rod 15, meanwhile, the culture dish 12 is fixed through the auxiliary fastening unit, the telescopic rod 15 continuously extends and enables the culture dish 12 to be separated from the step gap groove 20, the culture dish 12 corresponds to the gap on the concave platform 2 through the first sliding block 17, the telescopic rod 15 continuously extends and enables the culture dish 12 to be ejected out through the gap, the avoiding gap 21 can avoid the telescopic rod 15 when waiting for the next operation, the culture dish 12 on the upper-side dish rack 11 is ejected out by the groove supporting block 25, the baffle 29 is matched with the gap on the concave platform 2 and blocks the gap, so that the box body 1 is kept in a sealed state, when the telescopic rod 15 is extended continuously to push out the culture dish 12 through the gap, the synchronous opening unit opens the baffle plate 29 and exposes the gap, so that the culture dish 12 is pushed out smoothly, external air is prevented from entering the box body 1 to the maximum extent, the sealing performance is kept, when the telescopic rod 15 retracts, the baffle 29 is quickly reset to block the gap, so that the box body 1 is kept in a sealed state.

As shown in fig. 3 to 5, the sorting driving mechanism includes a driving bevel gear 23, a sliding unit is installed on one side of the box body 1, a rotating rod 14 is installed on the sliding unit through a rotating shaft sleeve 34 in a rotating manner, the driving bevel gear 23 is installed at one end of the rotating rod 14 in a sleeved manner, the other end of the driving bevel gear 23 extends to the outer side of the box body 1 and is provided with a first rotating disk 6, driven bevel gears 13 are installed on the top side of the lower-side vessel holder 10 and the bottom side of the upper-side vessel holder 11, the driving bevel gear 23 is alternatively meshed with the two driven bevel gears 13, the rotating rod 14 rotates on the rotating shaft sleeve 34 by rotating the first rotating disk 6 through the arrangement of the driving bevel gear 23, the rotating shaft sleeve 34 and the rotating rod 14 are moved through the sliding unit, the driving bevel gear 23 is meshed with one of the driven bevel gears 13, and the corresponding lower-side vessel holder 10 or upper-side vessel holder 11 rotates, so as to realize a driving function.

As shown in fig. 4 and 5, the sliding unit includes a sliding plate 24, one side of the box body 1 is provided with a mounting hole and is provided with a chute frame 7, the sliding plate 24 is slidably mounted on the chute frame 7, a rotating shaft sleeve 34 is mounted on the sliding plate 24, a transverse push rod 8 is mounted on one side of the rotating shaft sleeve 34, a fixing unit is mounted on one side of the transverse push rod 8, a sealing unit is mounted on one side of the chute frame 7, the sliding plate 24 is made to slide on the chute frame 7 by pushing the transverse push rod 8 through the arrangement of the sliding plate 24, so that the position of the rotating rod 14 is changed, and after the sliding plate slides to a corresponding position, the sliding plate is locked through the fixing unit.

As shown in fig. 5, the fixing unit includes a plug 36, one side of the transverse push rod 8 is provided with a slide hole, the plug 36 is slidably mounted in the slide hole, one side of the box body 1 is correspondingly provided with two limit slots 35, one end of the plug 36 is matched with the corresponding limit slot 35, the two limit slots 35 correspond to the two driven bevel gears 13, and by setting the plug 36, after the sliding plate 24 slides to the corresponding position, one end of the plug 36 slides into the corresponding limit slot 35 through the slide hole, so as to limit the relative position of the sliding plate 24 and the box body 1, and thus, the fixing function is realized.

As shown in fig. 5, the sealing unit includes an elastic sealing film 5, the elastic sealing film 5 is sleeved on the rotating shaft sleeve 34, the periphery of the elastic sealing film 5 is installed on one side of the chute frame 7, the elastic sealing film 5 has certain elasticity and deforms when the rotating rod 14 moves, so that the elastic sealing film 5 can always cover one side of the chute frame 7, the sealing of the chute frame 7 is maintained, and the sealing performance of the box body 1 is maintained.

As shown in fig. 2, the driving unit includes a threaded rod 19, a threaded hole is opened on one side of the first slider 17, a fixing frame is installed on the inner wall of the bottom side of the box body 1, the threaded rod 19 is rotatably installed on the fixing frame, the threaded rod 19 is matched with the threaded hole, one end of the threaded rod 19 extends to the outer side of the box body 1 and is sleeved with the second rotating disc 4, the threaded rod 19 rotates on the fixing frame through the second rotating disc 4, and in the rotating process, the first slider 17 synchronously moves, so that the position of the telescopic rod 15 is driven to move, and the driving function is realized.

As shown in fig. 6, the synchronous opening unit includes a wedge block 30, a second slide rail 32 is installed on the bottom side of the recessed platform 2, a baffle 29 is installed on the second slide rail 32 through a second slide block 33, one side of the wedge block 30 is installed on one side of the baffle 29, a roller 28 is installed on one side of the groove support block 25 through a support 27 in a rotating mode, the roller 28 is matched with the inclined surface of the wedge block 30, a resetting unit is installed on one side of the wedge block 30, through the arrangement of the wedge block 30, in the process of extending the telescopic rod 15, the roller 28 is in contact with the inclined surface of the wedge block 30, and the wedge block 30 is pushed, so that the wedge block 30 slides along the second slide rail 32 to one side far away from the telescopic rod 15, and drives the baffle 29 to slide together, so that the gap is exposed, and the synchronous opening function is realized.

As shown in fig. 6, the reset unit includes a spring 31, one end of the spring 31 is installed on one side of the wedge block 30, the other end is installed on the inner side wall of the box body 1, through the setting of the spring 31, when the wedge block 30 is exposed to one side of the telescopic rod 15 and slides, one side of the wedge block 30 compresses the spring 31, when the telescopic rod 15 is shortened, the spring 31 pushes the wedge block 30 to slide to one side close to the telescopic rod 15, and the gap is sealed, so that the reset function is realized.

As shown in fig. 2 and 6, the auxiliary fastening unit includes a piston cylinder 3, a suction cup 26 is installed in the groove of the groove holder 25, the suction cup 26 is matched with the bottom side corresponding to the culture dish 12, the piston cylinder 3 is communicated with the suction cup 26 through an air pipe 16, a negative pressure is formed in the cylinder by operating the piston cylinder 3, a negative pressure is formed simultaneously on one side of the suction cup 26 communicated with the piston cylinder 3 through the air pipe 16, an adsorption force is formed on the culture dish 12, so that the culture dish 12 is fixed, the stability is increased, the piston cylinder 3 is reversely operated, the negative pressure disappears, and the suction cup 26 is easy to separate the culture dish 12.

A method for culturing a microbial culture device with high sealing performance, which comprises the following steps:

s1, during normal culture, the upper cover plate 37 seals the box body 1, the interior of the box body 1 is set to be a specific environment, culture media are placed in the culture dishes 12 and microorganisms are cultured, and the plurality of stepped notch grooves 20 in the lower side dish frame 10 and the upper side dish frame 11 can bear a plurality of culture dishes 12 and culture the microorganisms simultaneously;

s2, when the microorganisms need to be checked or observed, the upper cover plate 37 is opened, the lower side vessel frame 10 or the upper side vessel frame 11 is selectively driven to rotate through the sorting driving mechanism, and the culture vessel 12 needing to be taken out is rotated to a position corresponding to the notch on the concave platform 2;

s3, moving the telescopic rod 15 through the driving unit to enable the groove support block 25 to correspond to the culture dish 12 to be taken out, enabling the groove support block 25 to support the bottom side of the culture dish 12 through extension of the telescopic rod 15, fixing the culture dish 12 through the auxiliary fastening unit, enabling the telescopic rod 15 to extend continuously and enabling the culture dish 12 to be separated from the step notch groove 20, enabling the culture dish 12 to correspond to a notch on the concave platform 2 through the first sliding block 17, enabling the telescopic rod 15 to extend continuously and enabling the culture dish 12 to be ejected out through the notch to wait for next operation;

s4, the avoidance notch 21 can avoid the telescopic rod 15, the culture dish 12 on the upper side dish rack 11 is ejected out by the groove support block 25, the baffle 29 is matched with the notch on the sunken platform 2 and blocks the notch, the box body 1 is kept in a sealing state, when the telescopic rod 15 is extended continuously to enable the culture dish 12 to be ejected out through the notch, the synchronous opening unit enables the baffle 29 to be opened and the notch to be exposed, the culture dish 12 is ejected out smoothly, the external air is prevented from entering the box body 1 to the maximum degree, the sealing performance is kept, when the telescopic rod 15 retracts, the baffle 29 is reset rapidly to block the notch, and the box body 1 is kept in the sealing state.

The working principle of the invention is as follows:

during normal culture, the upper cover plate 37 seals the box body 1, the interior of the box body 1 is arranged in a specific environment, culture media are placed in the culture dishes 12 to culture microorganisms, the plurality of stepped notch grooves 20 on the lower-side dish rack 10 and the upper-side dish rack 11 can bear a plurality of culture dishes 12 to simultaneously carry out microorganism culture, when the microorganisms need to be checked or observed, the upper cover plate 37 is opened, the lower-side dish rack 10 or the upper-side dish rack 11 is selectively driven to rotate through the sorting driving mechanism, the culture dishes 12 needing to be taken out are rotated to the positions corresponding to the notches on the concave platform 2, the telescopic rod 15 is moved through the driving unit, the groove support block 25 corresponds to the culture dishes 12 needing to be taken out, the bottom sides of the culture dishes 12 are supported by the groove support block 25 through extension of the telescopic rod 15, and the culture dishes 12 are fixed through the auxiliary fastening unit, the telescopic rod 15 continues to extend and enables the culture dish 12 to be separated from the step gap groove 20, the culture dish 12 corresponds to a gap on the concave platform 2 through the first sliding block 17, the telescopic rod 15 continues to extend and enables the culture dish 12 to be ejected out through the gap, the next operation is waited, the avoiding gap 21 can avoid the telescopic rod 15, the groove support block 25 can eject the culture dish 12 on the upper-side dish frame 11, the baffle plate 29 is matched with the gap on the concave platform 2 and blocks the gap, the box body 1 is kept in a sealing state, when the telescopic rod 15 continues to extend and enables the culture dish 12 to be ejected out through the gap, the synchronous opening unit enables the baffle plate 29 to be opened and enables the gap to be exposed, the culture dish 12 is ejected out smoothly, external air is prevented from entering the box body 1 to the greatest extent, the sealing performance is kept, when the telescopic rod 15 retracts, the baffle plate 29 is quickly reset and blocks the gap, and the box body 1 is kept in a sealing state.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. The utility model provides a microorganism culture apparatus that leakproofness is strong, includes box (1), its characterized in that: the top side of the box body (1) is provided with a sunken platform (2), the top surface of the sunken platform (2) is provided with a notch, the bottom side of the sunken platform (2) is slidably provided with a baffle (29) matched with the notch, an upper cover plate (37) is arranged above the sunken platform (2), the box body (1) is internally provided with a fixed cylinder (9), the fixed cylinder (9) is rotatably provided with a lower-side vessel frame (10) and an upper-side vessel frame (11) through a damping ring (22), the lower-side vessel frame (10) and the upper-side vessel frame (11) are uniformly provided with a plurality of step notch grooves (20), a plurality of culture vessels (12) are respectively placed on the step notch grooves (20), the notch on the sunken platform (2) is matched with the culture vessels (12), one side of the lower-side vessel frame (10) is provided with an avoiding notch (21), one sides of the lower-side vessel frame (10) and the upper-side of the upper-side vessel frame (11) are provided with a sorting driving mechanism, the inner wall of the box body (1) is provided with a first slide rail (18), one side of the first slide rail (17) is provided with a first slide block (17), and a first slide block (17) is arranged on the top end of the telescopic rod (17), the top side of the groove support block (25) is matched with the bottom side of the culture dish (12), an auxiliary fastening unit is installed in the groove of the groove support block (25), a synchronous opening unit is installed on one side of the groove support block (25), and the synchronous opening unit is matched with the baffle (29).

2. The microbial cultivation device of claim 1, wherein: sorting drive mechanism includes drive bevel gear (23), the slip unit is installed to one side of box (1), rotate through pivot cover (34) on the slip unit and install dwang (14), drive bevel gear (23) cup joint and install the one end of dwang (14), the other end extends to the outside of box (1) is installed first rotary disk (6), the top side of downside vessel frame (10) with driven bevel gear (13) are all installed to the bottom side of upside vessel frame (11), drive bevel gear (23) and two driven bevel gear (13) mesh in turn matches.

3. The microbial cultivation device of claim 2, wherein: the sliding unit comprises a sliding plate (24), one side of the box body (1) is provided with a mounting hole and is provided with a sliding groove frame (7), the sliding plate (24) is slidably mounted on the sliding groove frame (7), the rotating shaft sleeve (34) is mounted on the sliding plate (24), one side of the rotating shaft sleeve (34) is provided with a transverse push rod (8), one side of the transverse push rod (8) is provided with a fixing unit, and one side of the sliding groove frame (7) is provided with a sealing unit.

4. The microbial cultivation device of claim 3, wherein: the fixing unit comprises a bolt (36), a sliding hole is formed in one side of the transverse push rod (8), the bolt (36) is slidably mounted in the sliding hole, two limiting slots (35) are correspondingly mounted in one side of the box body (1), one end of the bolt (36) is matched with the corresponding limiting slots (35), and the two limiting slots (35) correspond to the two driven bevel gears (13).

5. The microorganism culture apparatus with high sealing performance as claimed in claim 3, wherein: the sealing unit comprises an elastic sealing film (5), the elastic sealing film (5) is sleeved on the rotating shaft sleeve (34), and the peripheral side of the elastic sealing film (5) is installed on one side of the chute frame (7).

6. The microbial cultivation device of claim 5, wherein: the drive unit comprises a threaded rod (19), a threaded hole is formed in one side of the first sliding block (17), a fixing frame is installed on the inner wall of the bottom side of the box body (1), the threaded rod (19) is installed on the fixing frame in a rotating mode, the threaded rod (19) is matched with the threaded hole, and one end of the threaded rod (19) extends to the outer side of the box body (1) and is provided with a second rotating disc (4) in a sleeved mode.

7. The microorganism culture apparatus with high sealing performance as claimed in claim 1, wherein: the unit of opening in step includes wedge (30), second slide rail (32) are installed to the bottom side of sunken platform (2), install baffle (29) through second slider (33) on second slide rail (32), install one side of wedge (30) one side of baffle (29), rotate through support (27) one side of recess tray (25) and install gyro wheel (28), gyro wheel (28) with the inclined plane phase-match of wedge (30), the unit that resets is installed to one side of wedge (30).

8. The microbial cultivation device of claim 7, wherein: the reset unit comprises a spring (31), one end of the spring (31) is installed on one side of the wedge-shaped block (30), and the other end of the spring is installed on the inner side wall of the box body (1).

9. The microbial cultivation device of claim 1, wherein: the auxiliary fastening unit comprises a piston cylinder (3), a sucker (26) is installed in a groove of the groove supporting block (25), the sucker (26) is matched with the bottom side of the corresponding culture dish (12), and the piston cylinder (3) is communicated with the sucker (26) through an air pipe (16).

10. A method for cultivating a highly sealed microorganism culture apparatus according to any one of claims 1 to 9, the method comprising:

s1, during normal culture, an upper cover plate (37) seals the box body (1), a specific environment is arranged inside the box body (1), a culture medium is placed in the culture dish (12) and microorganisms are cultured, and a plurality of step gap grooves (20) in the lower side dish frame (10) and the upper side dish frame (11) can bear a plurality of culture dishes (12) and culture the microorganisms simultaneously;

s2, when microorganisms need to be checked or observed, the upper cover plate (27) is opened, the lower side vessel frame (10) or the upper side vessel frame (11) is selectively driven to rotate through the sorting driving mechanism, and the culture vessel (12) needing to be taken out is rotated to a position corresponding to the notch on the concave platform (2);

s3, moving a telescopic rod (15) through a driving unit, enabling a groove supporting block (25) to correspond to the culture dish (12) to be taken out, enabling the groove supporting block (25) to support the bottom side of the culture dish (12) through extension of the telescopic rod (15), fixing the culture dish (12) through an auxiliary fastening unit, enabling the telescopic rod (15) to extend continuously and enabling the culture dish (12) to be separated from a step gap groove (20), enabling the culture dish (12) to correspond to a gap in the concave platform (2) through the first sliding block (17), enabling the telescopic rod (15) to extend continuously, enabling the culture dish (12) to be ejected through the gap, and waiting for next operation;

s4, the avoidance notch (21) can avoid the telescopic rod (15), the groove supporting block (25) ejects the culture dish (12) located on the upper side dish frame (11), the baffle (29) is matched with the notch on the sunken platform (2) and blocks the notch, so that the box body (1) is kept in a sealing state, when the telescopic rod (15) is continuously extended to enable the culture dish (12) to be ejected out through the notch, the synchronous opening unit enables the baffle (29) to be opened and enables the notch to be exposed, the culture dish (12) is smoothly ejected out, external air is prevented from entering the interior of the box body (1) to the maximum degree, the sealing performance is kept, and when the telescopic rod (15) retracts, the baffle (29) is rapidly reset to block the notch, so that the box body (1) is kept in the sealing state.