CN117343826B - Microorganism culture equipment - Google Patents
Microorganism culture equipment Download PDFInfo
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- CN117343826B CN117343826B CN202311644990.8A CN202311644990A CN117343826B CN 117343826 B CN117343826 B CN 117343826B CN 202311644990 A CN202311644990 A CN 202311644990A CN 117343826 B CN117343826 B CN 117343826B
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- 244000005700 microbiome Species 0.000 title claims abstract description 46
- 230000007246 mechanism Effects 0.000 claims abstract description 19
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 230000007723 transport mechanism Effects 0.000 claims abstract description 9
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims description 71
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
- 239000010935 stainless steel Substances 0.000 claims description 4
- 238000013461 design Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 10
- 238000000034 method Methods 0.000 description 23
- 230000008569 process Effects 0.000 description 21
- 239000001963 growth medium Substances 0.000 description 6
- 230000000903 blocking effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000002689 soil Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of microorganism culture, in particular to microorganism culture equipment, which comprises a constant temperature incubator; the constant temperature incubator comprises a box body, a box door, a heating device, a temperature sensor, a humidity adjusting device, an observation window, a light source, a control system and an operation panel; a culture mechanism and a transport mechanism are arranged in the box body; the top of the box body is provided with a picking and placing mechanism; the culture mechanism comprises a rotating assembly and a placing assembly; the rotating component is arranged in the box body; the placing component is arranged on the rotating component; the transport mechanism comprises a guide assembly and a transport assembly; the guide component is arranged in the box body and is positioned at the left side of the rotating component; a transport assembly is arranged in the guide assembly; the picking and placing mechanism comprises a picking and placing bin; the invention is mainly used for solving the problem that when the box door is opened, external gas can enter the incubator, so that the environment for culturing microorganisms in the incubator is easy to change, thereby influencing the culturing effect of the microorganisms.
Description
Technical Field
The invention belongs to the technical field of microorganism culture, and particularly relates to microorganism culture equipment.
Background
Microorganism culture refers to the rapid growth and propagation of certain microorganisms by means of artificially prepared culture media and artificially created culture conditions (such as culture temperature and the like), and is called microorganism culture. Microorganism culture can be classified into pure culture and mixed culture, the former means that purified single strain is cultured and utilized; the latter means that the microorganism in the mixed strain or natural sample (such as soil) is cultivated, and then the diversity and the number of the microorganism in the soil can be estimated to a certain extent according to the variety and the number of the microorganism growing on the culture medium;
the constant temperature incubator is a common device for culturing microorganisms, is also called a water-proof electrothermal cell (mould) incubator, and is used for bacterial culture, breeding, fermentation and other constant temperature tests in scientific research departments such as medical and health, medical industry, biochemistry, industrial production, agricultural science and the like;
when the constant temperature incubator is used for culturing microorganisms, the growth condition of the microorganisms needs to be checked regularly, so that the culture period of the microorganisms is adjusted in real time;
however, when the growth condition of the microorganism is periodically checked, a worker is required to open the box door, then take out the culture medium, sample the culture medium, and then check the growth condition of the microorganism, when the box door is opened, external gas can enter the incubator, and the environment in which the microorganism is cultured in the incubator is easily changed, so that the culture effect of the microorganism is affected.
Disclosure of Invention
In order to make up for the deficiency of the prior art, solve the above-mentioned technical problem; the invention provides microorganism culture equipment.
The technical scheme adopted for solving the technical problems is as follows: the microorganism culture equipment comprises a constant temperature incubator; the constant temperature incubator comprises a box body, a box door, a heating device, a temperature sensor, a humidity adjusting device, an observation window, a light source, a control system and an operation panel;
a culture mechanism and a transport mechanism are arranged in the box body; the top of the box body is provided with a picking and placing mechanism;
the culture mechanism comprises a rotating assembly and a placing assembly; the rotating component is arranged in the box body and is used for rotating the placing component; the placing component is arranged on the rotating component and is used for placing a culture dish for culturing microorganisms;
the transport mechanism comprises a guide assembly and a transport assembly; the guide component is arranged in the box body and is positioned at the left side of the rotating component; a transport assembly is arranged in the guide assembly and is used for transporting the culture dish;
the picking and placing mechanism comprises a picking and placing bin; the picking and placing bin is arranged at the top of the box body; the taking and placing bin is provided with a bin door;
the rotating assembly comprises two rotating rods; the two rotating rods rotate in the box body; the rotating rod far away from the picking and placing bin extends to the top of the box body and is fixedly connected with a first crank;
the outer ring surfaces of the two rotating rods are fixedly connected with evenly arranged chain wheels; the two opposite chain wheels on the two rotating rods are respectively connected with a chain in a rotating way;
the placement assembly comprises a uniformly arranged mounting plate; each mounting plate is fixedly connected with a chain; a placing ring is fixedly connected to one side of the mounting plate, which is not fixedly connected with the chain; the placing ring is provided with a placing table which is used for placing the culture dish;
a notch is formed in one side, far away from the mounting plate, of the placement ring, and penetrates through the placement ring;
the guide assembly comprises a rectangular guide bin; the rectangular guide bin is positioned at the left side of the chain and fixedly connected with the side wall of the box body; the rectangular guide bin extends to the upper part of the box body and is positioned in the picking and placing bin;
a round guide cylinder is fixedly connected to one side of the rectangular guide bin, which faces the chain; the top of the circular guide cylinder is attached to the box body; the top of the circular guide cylinder is provided with a circular groove on the box body, and the circular groove is positioned in the picking and placing bin;
one end of the rectangular guide bin, which faces the circular guide cylinder, is of an open type design; a slideway is arranged on one side of the circular guide cylinder, which faces the rectangular guide bin, and the width of the slideway is the same as the width of the inner cavity of the rectangular guide bin; the round guide cylinder is provided with uniformly arranged transportation openings in the vertical direction; the number of the conveying ports is the same as that of the chains;
the transport assembly includes a lead screw; the screw rod rotates in the rectangular guide bin; the top of the screw rod extends to the top of the picking and placing bin and is fixedly connected with a second crank;
the rectangular guide bin is slidably connected with a first sliding block, and the first sliding block is tightly attached to the rectangular guide bin and is in threaded engagement with the lead screw through threads; the first sliding block extends into the slideway and is attached to the slideway; the first sliding block is fixedly connected with a fixing plate, and the width of the fixing plate is slightly smaller than that of the notch; a tray is fixedly connected to one side, far away from the first sliding block, of the fixing plate, and the diameter of the tray is slightly smaller than the diameter of the inner ring of the placing table in the placing ring;
a second sliding block is slidably connected below the first sliding block in the rectangular guide bin, and extends into the slideway; the first sliding block is connected with the second sliding block through a connecting rod; the second sliding block is in threaded engagement with the lead screw through threads;
the second sliding block is fixedly connected with a first sealing disc, and the first sealing disc is positioned in the circular guide cylinder and is tightly attached to the circular guide cylinder;
a third sliding block is slidably connected above the first sliding block and is slidably connected on the screw rod; the third sliding block extends into the slideway; the third sliding block is fixedly connected with a second sealing disc, and the second sealing disc is positioned in the circular guide cylinder above the uppermost transportation port and is tightly attached to the circular guide cylinder;
the first sliding block and the third sliding block are connected through an elastic band; the upper surface of the third sliding block is fixedly connected with a pull rope, and the other end of the pull rope is fixedly connected to the top of the rectangular guide bin.
Preferably, a first motor is arranged in the box body; the first motor is connected with one of the rotating rods through a coupler; the lower part of the rectangular guide bin is positioned in the box body and fixedly connected with a second motor, and the second motor is connected with a screw rod through a coupler; the first motor and the second motor are controlled by a control system.
Preferably, the lower surface of the second sealing disc is fixedly connected with uniformly arranged limiting rods, and a plurality of limiting rods are enclosed into a circle;
and one side of the limiting rod, which is far away from the second sealing disc, is bent outwards.
Preferably, the upper surface of the third sliding block is fixedly connected with a spring, and the spring extends upwards around the screw rod and is fixedly connected with the top of the rectangular guide bin; the pull rope is staggered with the spring;
the circular guide cylinder is made of stainless steel with heat conducting performance;
the second sliding block, the third sliding block, the first sealing disk and the side face of the second sealing disk are fixedly connected with sealing layers.
The beneficial effects of the invention are as follows:
1. according to the microorganism culture equipment, the culture medium is taken and placed by the conveying mechanism, so that external gas can be prevented from entering the box body, the environment for culturing microorganisms in the constant-temperature incubator is changed, the culture effect of the microorganisms is affected, meanwhile, the gas between the first sealing disc and the second sealing disc can be prevented from directly entering the box body through heat exchange of the placed culture dishes, the culture environment in the box body is changed, the culture effect of the microorganisms is affected, and meanwhile, the rotating rod can be rotated to drive the culture dishes to rotate, so that the position of the culture dishes in the box body can be changed, the culture dishes can be better contacted with the culture environment in the box body, and the culture effect of the microorganisms is improved.
2. According to the microorganism culture equipment, the limiting rods fixedly connected with the lower surface of the second sealing disc form a round shape, one side of each limiting rod far away from the second sealing disc is bent outwards, the limiting rods can limit the culture dish in the process of upwards moving the culture dish, the culture dish is located among the limiting rods, the condition that the culture dish is turned over due to the fact that the culture dish slides on the tray when the tray moves upwards is avoided, meanwhile, when a worker places the inspected culture dish on the tray again, the worker pushes the second sealing disc upwards, then places the culture dish on the tray, then when the limiting rods are driven by the second sealing disc to downwards move, the culture dish can be pushed to move, the culture dish is located among the limiting rods again, in the process, the culture dish can be limited, the culture dish can be placed down at the same position as the culture dish when the culture dish is taken out, and the culture dish can enter the placing table of the placing ring better.
Drawings
The invention is further described below with reference to the accompanying drawings.
FIG. 1 is a perspective view of the entire present invention;
FIG. 2 is an internal block diagram of the present invention;
FIG. 3 is an enlarged view of a portion of FIG. 2A in accordance with the present invention;
FIG. 4 is a structural view of the culture mechanism and the transport mechanism in the present invention;
FIG. 5 is an enlarged view of a portion of the invention at B in FIG. 4;
FIG. 6 is an enlarged view of a portion of the invention at C in FIG. 4;
FIG. 7 is a block diagram of a rectangular guide cartridge and a circular guide cylinder in the present invention;
FIG. 8 is an enlarged view of a portion of the invention at D in FIG. 7;
FIG. 9 is a block diagram of a dish placed in the placement module of the present invention;
FIG. 10 is a block diagram of a placement module of the present invention;
FIG. 11 is a top view of the present invention;
FIG. 12 is a cross-sectional view taken along line E-E of FIG. 11 in accordance with the present invention;
FIG. 13 is an enlarged view of a portion of FIG. 12 at F in accordance with the present invention;
fig. 14 is an enlarged view of a portion of fig. 12 at G in accordance with the present invention.
In the figure: 1. a case; 11. a door; 12. taking and placing a bin; 13. a bin gate; 14. a culture dish; 15. a circular groove; 2. a rotating lever; 21. a first crank; 22. a sprocket; 23. a chain; 24. a mounting plate; 25. placing a ring; 26. a placement table; 27. a groove is formed; 28. a first motor; 3. a rectangular guide bin; 31. a screw rod; 311. a second motor; 32. a second crank; 33. a first slider; 331. a connecting rod; 34. a fixing plate; 35. a tray; 36. a second slider; 361. an elastic band; 37. a first sealing disk; 38. a third slider; 39. a second sealing plate; 391. a pull rope; 392. a limit rod; 4. a circular guide cylinder; 41. a slideway; 42. and a transport port.
Detailed Description
The invention is further described in connection with the following detailed description in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
As shown in fig. 1 to 14, a microorganism culture apparatus according to the present invention;
example 1
Comprises a constant temperature incubator; the constant temperature incubator comprises a box body 1, a box door 11, a heating device, a temperature sensor, a humidity adjusting device, an observation window, a light source, a control system and an operation panel;
a culture mechanism and a transport mechanism are arranged in the box body 1; a picking and placing mechanism is arranged at the top of the box body 1;
the culture mechanism comprises a rotating assembly and a placing assembly; the rotating component is arranged in the box body 1 and is used for rotating the placing component; the placing component is arranged on the rotating component and is used for placing a culture dish 14 for culturing microorganisms;
the transport mechanism comprises a guide assembly and a transport assembly; the guide component is arranged in the box body 1 and is positioned at the left side of the rotating component; a transport assembly is mounted in the guide assembly and is used for transporting the culture dish 14;
the picking and placing mechanism comprises a picking and placing bin 12; the picking and placing bin 12 is arranged at the top of the box body 1; a bin door 13 is arranged on the taking and placing bin 12;
the rotating assembly comprises two rotating rods 2; the two rotating rods 2 rotate in the box body 1; the rotating rod 2 far away from the picking and placing bin 12 extends to the top of the box body 1 and is fixedly connected with a first crank 21;
the outer ring surfaces of the two rotating rods 2 are fixedly connected with evenly arranged chain wheels 22; the two opposite chain wheels 22 on the two rotating rods 2 are respectively and rotatably connected with a chain 23;
the placement assembly includes a uniformly arranged mounting plate 24; each mounting plate 24 is fixedly connected with a chain 23; a placing ring 25 is fixedly connected to one side of the mounting plate 24, which is not fixedly connected with the chain 23; the placing ring 25 is provided with a placing table 26, and the placing table 26 is used for placing the culture dish 14;
a notch 27 is formed in one side, far away from the mounting plate 24, of the placement ring 25, and the notch 27 penetrates through the placement ring 25;
the guide assembly comprises a rectangular guide bin 3; the rectangular guide bin 3 is positioned at the left side of the chain 23 and is fixedly connected with the side wall of the box body 1; the rectangular guide bin 3 extends to the upper part of the box body 1 and is positioned in the taking and placing bin 12;
a circular guide cylinder 4 is fixedly connected to one side, facing the chain 23, of the rectangular guide bin 3; the top of the circular guide cylinder 4 is attached to the box body 1; the top of the circular guide cylinder 4 is provided with a circular groove 15 on the box body 1, and the circular groove 15 is positioned in the picking and placing bin 12;
one end of the rectangular guide bin 3, which faces the circular guide cylinder 4, is of an open type design; a slideway 41 is arranged on one side of the circular guide cylinder 4 facing the rectangular guide bin 3, and the width of the slideway 41 is the same as the width of the inner cavity of the rectangular guide bin 3; the round guide cylinder 4 is provided with evenly arranged transportation openings 42 in the vertical direction; the number of the transport openings 42 is the same as the number of the chains 23;
the transport assembly includes a lead screw 31; the screw rod 31 rotates in the rectangular guide bin 3; the top of the lead screw 31 extends to the top of the picking and placing bin 12 and is fixedly connected with a second crank 32;
the rectangular guide bin 3 is connected with a first sliding block 33 in a sliding way, and the first sliding block 33 is tightly attached to the rectangular guide bin 3 and is in threaded engagement with the lead screw 31 through threads; the first sliding block 33 extends into the slideway 41 and is attached to the slideway 41; the first sliding block 33 is fixedly connected with a fixing plate 34, and the width of the fixing plate 34 is slightly smaller than the width of the notch 27; a tray 35 is fixedly connected to one side, far away from the first sliding block 33, of the fixing plate 34, and the diameter of the tray 35 is slightly smaller than the diameter of the inner ring of the placing table 26 in the placing ring 25;
a second sliding block 36 is slidably connected below the first sliding block 33 in the rectangular guide bin 3, and the second sliding block 36 extends into the slideway 41; the first sliding block 33 is connected with the second sliding block 36 through a connecting rod 331; the second sliding block 36 is in threaded engagement with the lead screw 31 through threads;
the second sliding block 36 is fixedly connected with a first sealing disc 37, and the first sealing disc 37 is positioned in the circular guide cylinder 4 and is tightly attached to the circular guide cylinder 4;
a third sliding block 38 is slidably connected above the first sliding block 33 and above the rectangular guide bin 3, and the third sliding block 38 slides on the lead screw 31; the third slider 38 extends into the slideway 41; the third sliding block 38 is fixedly connected with a second sealing disc 39, and the second sealing disc 39 is positioned in the circular guide cylinder 4 above the uppermost transportation port 42 and is tightly attached to the circular guide cylinder 4;
the first sliding block 33 and the third sliding block 38 are connected through an elastic band 361; the upper surface of the third sliding block 38 is fixedly connected with a pull rope 391, and the other end of the pull rope 391 is fixedly connected to the top of the rectangular guide bin 3;
in the process of culturing microorganisms by using the constant temperature incubator, if the growth condition of the microorganisms needs to be checked, at the moment, a worker rotates the second crank 32, the second crank 32 drives the screw rod 31 to rotate, and in the process of rotating the screw rod 31, the first slide block 33 and the second slide block 36 are driven to move downwards in the rectangular guide bin 3 and the slideway 41 due to the fact that the first slide block 33 and the second slide block 36 are in threaded engagement with the screw rod 31, meanwhile, the first slide block 33 drives the fixing plate 34 and the tray 35 to move downwards in the circular guide cylinder 4, meanwhile, the second slide block 36 drives the first sealing disc 37 to move downwards in the circular guide cylinder 4, and as the first slide block 33 is connected with the second slide block 36 through the connecting rod 331, the distance between the first slide block 33 and the second slide block 36 can be ensured to be always the same, because the third sliding block 38 is connected with the top of the rectangular guide bin 3 through the pull rope 391, and because the third sliding block 38 is connected with the first sliding block 33 through the elastic band 361, in the process that the first sliding block 33 moves downwards, the second sealing disc 39 is pulled by the pull rope 391 and cannot be pulled downwards by the elastic band 361 to move downwards, so that the second sealing disc 39 is positioned in the circular guide cylinder 4 above the uppermost transportation port 42, the circular guide cylinder 4 is sealed in a blocking manner, the gas in the taking and placing bin 12 is prevented from entering the box body 1 through the circular guide cylinder 4, meanwhile, the third sliding block 38 can seal the rectangular guide bin 3 and the sliding way 41 in a blocking manner, and when the fixed plate 34 and the tray 35 move downwards to the bottom of the transportation port 42, a worker stops rotating the second crank 32 at the moment;
then, a worker rotates the first crank 21, the first crank 21 drives the rotating rod 2 to rotate, in the process of rotating the rotating rod 2, the chain wheel 22 is driven to rotate, meanwhile, the chain wheel 22 drives the chain 23 to rotate, when the chain 23 rotates, the chain wheel 22 on the other rotating rod 2 and the rotating rod 2 are driven to rotate, meanwhile, the mounting plate 24 and the placing ring 25 are driven to rotate, and as the culture dish 14 is placed on the placing table 26 of the placing ring 25, the culture dish 14 is driven to rotate, and when the culture dish 14 to be inspected is rotated into the transport port 42 formed in the circular guide cylinder 4, the rotation of the first crank 21 is stopped at the moment;
then the worker rotates the second crank 32 reversely, the first slider 33 and the second slider 36 are driven to move upwards in the process of rotating the second crank 32, the fixed plate 34 and the tray 35 are driven to move upwards gradually in the process of moving the first slider 33 upwards, the second slider 36 drives the first sealing plate 37 to move upwards at the same time, in the process of moving the fixed plate 34 and the tray 35 upwards gradually, the width of the fixed plate 34 is slightly smaller than the width of the notch 27, the diameter of the tray 35 is slightly smaller than the diameter of the inner ring of the placing table 26 in the placing ring 25, so that the fixed plate 34 gradually stretches into the notch 27 from the bottom gradually, simultaneously, the tray 35 gradually stretches into the placement ring 25 from the bottom, then the top of the tray 35 is contacted with the culture dish 14, the culture dish 14 is driven to move upwards in the process that the fixing plate 34 and the tray 35 continuously move upwards, the culture dish 14 is separated from the placement table 26, when the tray 35 drives the culture dish 14 to move upwards to be above the placement ring 25, a worker continuously rotates the first crank 21, the first crank 21 drives the chain 23 to rotate through the rotating rod 2 and the chain wheel 22, meanwhile, the chain wheel 22 drives the placement ring 25 to rotate, the placement ring 25 is rotated out of the transport port 42, and when the placement ring 25 is rotated out of the transport port 42, the worker continuously rotates the second crank 32;
in the process of continuously rotating the second crank 32, the first slider 33, the second slider 36, the tray 35 and the first sealing disc 37 are driven to gradually move upwards through the lead screw 31, the culture dish 14 is gradually close to the second sealing disc 39 in the process of gradually moving upwards the culture dish 14, after the culture dish 14 is contacted with the lower surface of the second sealing disc 39, the first sealing disc 37 is moved upwards to be above the uppermost transport port 42, so that the round guide cylinder 4 can be sealed through the first sealing disc 37, the second sealing disc 39 is firstly moved upwards into the picking and placing bin 12 through the round groove 15 in the process of gradually moving upwards the tray 35, the culture dish 14 and the first sealing disc 37, then the tray 35 is moved upwards into the picking and placing bin 12 with the culture dish 14, after the tray 35 and the culture dish 14 are moved upwards into the picking and placing bin 12, the rotation of the second crank 32 is stopped, the first sealing disc 37 is still positioned in the round guide cylinder 4, and the round guide cylinder 4 can be sealed, and meanwhile, the rectangular guide bin 3 and the slide way 41 can be sealed through the second slider 36;
then the staff opens the bin gate 13, takes the culture dish 14 out of the picking and placing bin 12, checks the growth condition of microorganisms in the culture dish 14, and because the first sealing disk 37 is still positioned in the circular guide cylinder 4 and seals the circular guide cylinder 4 in a blocking way, meanwhile, the second sliding block 36 can seal the rectangular guide bin 3 and the sliding way 41 in a blocking way, so that the air entering the picking and placing bin 12 from the outside can be prevented from entering the box body 1 through the circular guide cylinder 4 and the rectangular guide bin 3, after the staff checks, the staff opens the bin gate 13 again, places the culture dish 14 after the checking is finished on the tray 35, then the staff closes the bin gate 13 and rotates the second crank 32, and in the process of rotating the second crank 32, the tray 35 and the first sealing disk 37 are driven to move downwards by the first sliding block 33 and the second sliding block 36 of the lead screw 31, simultaneously, the first sliding block 33 pulls the third sliding block 38 to move downwards through the elastic band 361, so as to drive the second sealing disk 39 to move downwards, when the second sealing disk 39 moves downwards into the circular guide cylinder 4 and the first sealing disk 37 is also positioned in the circular guide cylinder 4, the rotation of the second crank 32 is stopped, at the moment, the internal gas temperature of the box body 1 can exchange heat between the first sealing disk 37 and the second sealing disk 39, after a period of heat exchange, the staff continues to rotate the second crank 32, when the tray 35 drives the culture dish 14 to move downwards to the top position of the transport port 42, the rotation of the second crank 32 is stopped, then the first crank 21 drives the placing ring 25 for placing the culture dish 14 to rotate to the lower side of the tray 35 through the rotating rod 2 and the chain 23, when the placing ring 25 rotates to the lower side of the tray 35, at the moment, the rotation of the first crank 21 is stopped, the second crank 32 continues to rotate, so as to drive the tray 35 and the fixed plate 34 to gradually move downwards, the fixed plate 34 and the tray 35 gradually extend into the notch 27 and the placement ring 25 from the top in the process of moving downwards, when the fixed plate 34 and the supporting plate move downwards below the placement ring 25, the culture dish 14 is placed on the placement table 26 in the placement ring 25, and then the placement ring 25 is rotated out of the transport port 42 by a worker;
in the above process, the culture medium is taken and placed by utilizing the conveying mechanism, so that the outside air can be prevented from entering the box body 1, the environment for culturing microorganisms in the constant temperature incubator is changed, the culture effect of the microorganisms is affected, meanwhile, the air between the first sealing disc 37 and the second sealing disc 39 can be prevented from directly entering the box body 1 by exchanging heat with the placed culture dish 14, the culture environment in the box body 1 is changed, the culture effect of the microorganisms is affected, meanwhile, the rotating rod 2 can be rotated to drive the culture dish 14 to rotate, the position of the culture dish 14 in the box body 1 can be changed, the culture dish 14 is enabled to be better contacted with the culture environment in the box body 1, and the culture effect of the microorganisms is improved.
Another embodiment as the first embodiment
A first motor 28 is arranged in the box body 1; the first motor 28 is connected with one of the rotating rods 2 through a coupler; a second motor 311 is fixedly connected in the box body 1 below the rectangular guide bin 3, and the second motor 311 is connected with the screw rod 31 through a coupler; the first motor 28 and the second motor 311 are controlled by a control system;
meanwhile, the first motor 28 can be further installed to be connected with the rotating rod 2, the second motor 311 connected with the screw rod 31 is installed, when the microorganisms in the box body 1 need to be checked regularly, a worker can send out instructions through the operation panel, then the control system controls the first motor 28 and the second motor 311 to rotate through programs, the rotating rod 2 can be driven to rotate in the rotating process of the first motor 28, and accordingly the placing ring 25 can be indirectly driven to rotate to the position of the transport port 42, meanwhile, the screw rod 31 can be driven to rotate in the rotating process of the second motor 311, the culture dish 14 can be indirectly driven to move up and down in the rotating process of the screw rod 31, picking and placing can be carried out, and the worker does not need to rotate the first crank 21 and the second crank 32 in the process, so that the labor cost of the worker is saved.
Example two
The lower surface of the second sealing disc 39 is fixedly connected with uniformly arranged limiting rods 392, and a plurality of limiting rods 392 are enclosed into a circle;
the side of the limiting rod 392 away from the second sealing disc 39 is bent outwards;
because the gag lever post 392 that the lower surface of second sealing disk 39 linked firmly forms the circular, and the one side that the gag lever post 392 kept away from second sealing disk 39 is crooked outside, at the in-process that culture dish 14 moved up, gag lever post 392 can be spacing to culture dish 14, make culture dish 14 be located between a plurality of gag lever posts 392, thereby avoid tray 35 when culture dish 14 moved up, culture dish 14 slides on tray 35, make culture dish 14 appear the condition of turning on one's side, simultaneously when the staff put on tray 35 with the culture dish 14 after will examining, the staff promotes first upwards second sealing disk 39, then put culture dish 14 on tray 35, then when second sealing disk 39 drives gag lever post 392 and moves down, can promote culture dish 14 removal, make culture dish 14 be located between a plurality of gag lever posts 392 again, in-process can be spacing to culture dish 14, can make put down culture dish 14 and culture dish 14 in same position when taking out, thereby make culture dish 14 better get into place in placing the platform 26 of ring 25.
Example III
The upper surface of the third sliding block 38 is fixedly connected with a spring, and the spring extends upwards around the screw rod 31 and is fixedly connected with the top of the rectangular guide bin 3; the stay 391 is staggered with the spring;
the circular guide cylinder 4 is made of stainless steel with heat conducting property;
the second sliding block 36, the third sliding block 38 and the side surfaces of the first sealing disk 37 and the second sealing disk 39 are fixedly connected with sealing layers;
because the upper surface of the third sliding block 38 is fixedly connected with the spring, when the culture dish 14 pushes the second sealing disk 39 to move upwards, the second sealing disk 39 drives the third sliding block 38 to move upwards, so that the spring is compressed, the culture dish 14 can be further fixed in the process, and meanwhile, when the tray 35 drives the culture dish 14 to move downwards, the second sealing disk 39 moves downwards along with the culture dish 14 under the pushing force of the spring, so that the culture dish can move downwards without the pulling force of the elastic belt 361;
the circular guide cylinder 4 is made of stainless steel, so that the heat exchange effect on the gas between the first sealing disc 37 and the second sealing disc 39 can be improved;
the second sliding block 36, the third sliding block 38, the first sealing disc 37 and the second sealing disc 39 are fixedly connected with sealing layers on the side surfaces, so that the rectangular guide bin 3, the slideway 41 and the circular guide cylinder 4 can be better sealed.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (3)
1. A microorganism culture apparatus comprising a constant temperature incubator; the constant temperature incubator comprises a box body (1), a box door (11), a heating device, a temperature sensor, a humidity adjusting device, an observation window, a light source, a control system and an operation panel;
the device is characterized in that a culture mechanism and a transport mechanism are arranged in the box body (1); a picking and placing mechanism is arranged at the top of the box body (1);
the culture mechanism comprises a rotating assembly and a placing assembly; the rotating component is arranged in the box body (1) and is used for rotating the placing component; the placing component is arranged on the rotating component and is used for placing a culture dish (14) for culturing microorganisms;
the transport mechanism comprises a guide assembly and a transport assembly; the guide component is arranged in the box body (1) and is positioned at the left side of the rotating component; a transport assembly is arranged in the guide assembly and is used for transporting the culture dish (14);
the picking and placing mechanism comprises a picking and placing bin (12); the picking and placing bin (12) is arranged at the top of the box body (1); a bin door (13) is arranged on the taking and placing bin (12);
the guide assembly comprises a rectangular guide bin (3); the rectangular guide bin (3) is positioned at the left side of the chain (23) and is fixedly connected with the side wall of the box body (1); the rectangular guide bin (3) extends to the upper part of the box body (1) and is positioned in the taking and placing bin (12);
a circular guide cylinder (4) is fixedly connected to one side, facing the chain (23), of the rectangular guide bin (3); the top of the circular guide cylinder (4) is attached to the box body (1); the top of the circular guide cylinder (4) is provided with a circular groove (15) on the box body (1), and the circular groove (15) is positioned in the taking and placing bin (12);
one end of the rectangular guide bin (3) facing the circular guide cylinder (4) is of an open type design; a slideway (41) is arranged on one side of the circular guide cylinder (4) facing the rectangular guide bin (3), and the width of the slideway (41) is the same as the width of the inner cavity of the rectangular guide bin (3); the round guide cylinder (4) is provided with uniformly arranged transportation openings (42) in the vertical direction; the number of the conveying ports (42) is the same as the number of the chains (23);
the transport assembly comprises a lead screw (31); the screw rod (31) rotates in the rectangular guide bin (3); the top of the screw rod (31) extends to the top of the picking and placing bin (12) and is fixedly connected with a second crank (32);
a first sliding block (33) is connected in a sliding manner in the rectangular guide bin (3), and the first sliding block (33) is tightly attached to the rectangular guide bin (3) and is in threaded engagement with the lead screw (31) through threads; the first sliding block (33) extends into the slideway (41) and is attached to the slideway (41); a fixed plate (34) is fixedly connected to the first sliding block (33), and the width of the fixed plate (34) is slightly smaller than the width of the notch (27); a tray (35) is fixedly connected to one side, far away from the first sliding block (33), of the fixing plate (34), and the diameter of the tray (35) is slightly smaller than the diameter of the inner ring of the placing table (26) in the placing ring (25);
a second sliding block (36) is slidably connected below the first sliding block (33) and below the rectangular guide bin (3), and the second sliding block (36) extends into the slideway (41); the first sliding block (33) and the second sliding block (36) are connected through a connecting rod (331); the second sliding block (36) is in threaded engagement with the lead screw (31) through threads;
the second sliding block (36) is fixedly connected with a first sealing disc (37), and the first sealing disc (37) is positioned in the circular guide cylinder (4) and is tightly attached to the circular guide cylinder (4);
a third sliding block (38) is slidably connected above the first sliding block (33) and above the rectangular guide bin (3), and the third sliding block (38) slides on the lead screw (31); the third sliding block (38) extends into the slideway (41); a second sealing disc (39) is fixedly connected to the third sliding block (38), and the second sealing disc (39) is positioned in the circular guide cylinder (4) above the uppermost transportation port (42) and is tightly attached to the circular guide cylinder (4);
after the culture dish (14) is contacted with the lower surface of the second sealing disc (39), the first sealing disc (37) moves upwards to the upper part of the uppermost conveying port (42), and after the tray (35) and the culture dish (14) move upwards to the picking and placing bin (12), the first sealing disc (37) is still positioned in the circular guide cylinder (4);
the first sliding block (33) is connected with the third sliding block (38) through an elastic band (361); the upper surface of the third sliding block (38) is fixedly connected with a pull rope (391), and the other end of the pull rope (391) is fixedly connected to the top of the rectangular guide bin (3);
the lower surface of the second sealing disc (39) is fixedly connected with uniformly arranged limiting rods (392), and a plurality of limiting rods (392) are enclosed into a circle;
the side of the limiting rod (392) far away from the second sealing disc (39) is bent outwards;
the upper surface of the third sliding block (38) is fixedly connected with a spring, and the spring extends upwards around the screw rod (31) and is fixedly connected with the top of the rectangular guide bin (3); the stay rope (391) is staggered with the spring;
the circular guide cylinder (4) is made of stainless steel with heat conducting property;
the rotating assembly comprises two rotating rods (2); the two rotating rods (2) rotate in the box body (1); the rotating rod (2) far away from the picking and placing bin (12) extends to the top of the box body (1) and is fixedly connected with a first crank (21);
the outer ring surfaces of the two rotating rods (2) are fixedly connected with evenly arranged chain wheels (22); the two opposite chain wheels (22) on the two rotating rods (2) are respectively connected with a chain (23) in a rotating way;
the placement assembly includes a uniformly arranged mounting plate (24); each mounting plate (24) is fixedly connected with a chain (23); a placing ring (25) is fixedly connected to one side of the mounting plate (24) which is not fixedly connected with the chain (23); the placing ring (25) is provided with a placing table (26), and the placing table (26) is used for placing the culture dish (14);
a notch (27) is formed in one side, far away from the mounting plate (24), of the placement ring (25), and the notch (27) penetrates through the placement ring (25).
2. A microorganism culture apparatus according to claim 1, wherein: a first motor (28) is arranged in the box body (1); the first motor (28) is connected with one of the rotating rods (2) through a coupler; a second motor (311) is fixedly connected in the box body (1) below the rectangular guide bin (3), and the second motor (311) is connected with a screw rod (31) through a coupler; the first motor (28) and the second motor (311) are controlled by a control system.
3. A microorganism culture apparatus according to claim 2, wherein: the second sliding block (36), the third sliding block (38) and the side surfaces of the first sealing disc (37) and the second sealing disc (39) are fixedly connected with sealing layers.
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| CN202311644990.8A CN117343826B (en) | 2023-12-04 | 2023-12-04 | Microorganism culture equipment |
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