CN115786117A - Biological stem cell storage culture reagent case - Google Patents
Biological stem cell storage culture reagent case Download PDFInfo
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- CN115786117A CN115786117A CN202310046421.7A CN202310046421A CN115786117A CN 115786117 A CN115786117 A CN 115786117A CN 202310046421 A CN202310046421 A CN 202310046421A CN 115786117 A CN115786117 A CN 115786117A
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- 238000003860 storage Methods 0.000 title claims abstract description 91
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 20
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 20
- 238000007789 sealing Methods 0.000 claims abstract description 66
- 238000005192 partition Methods 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000012258 culturing Methods 0.000 claims description 12
- 238000003825 pressing Methods 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 2
- 238000010030 laminating Methods 0.000 abstract description 4
- 238000009434 installation Methods 0.000 description 6
- 230000002349 favourable effect Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 235000003869 genetically modified organism Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a biological stem cell storage and culture reagent box which comprises a box body and a partition plate arranged in the box body in a sliding manner, wherein the top of the partition plate is fixedly connected with a plurality of storage frames, and one side of the top of each storage frame is hinged with a sealing plate; the sealing plate is connected with the storage frame through a limiting assembly, a jacking assembly is assembled on the limiting assembly, a through groove is formed in one side of the box body, one end of the partition plate penetrates through the through groove and is fixedly connected with a pulling plate, and when the sealing plate covers the top of the storage frame, the limiting assembly locks the sealing plate and the storage frame; after the closing plate with store the frame laminating, can lock the closing plate through spacing subassembly, make its stable lid fit on storing the frame, and through the in-process that the arm-tie pulls out baffle and storage frame, exert pressure to the closing plate through the roof pressure subassembly for the closing plate is inseparable with the contact between the storage frame, thereby avoids outside air to enter into and causes the pollution to the culture dish in the holding vessel.
Description
Technical Field
The invention relates to the technical field of cell storage, in particular to a biological stem cell storage and culture reagent box.
Background
The biotechnology utilizes scientific technology and biological technology to modify agriculture or other aspects, scientifically utilizes modern genetic engineering technology to accurately select genes with certain excellent characteristics of organisms to be transferred to another species, and enables new genetically modified organisms to have expected specific characteristics.
Chinese patent CN213139539U discloses a biological stem cell storage culture reagent box which comprises a mounting box body, a placing plate and a placing mechanism; installing a box body: the front side of the box body is open, the inner wall of the installation box body is transversely and fixedly connected with two placing plates, and the bottom surface of the left side of the installation box body is fixedly connected with a support frame; a placement mechanism: contain backup pad, connecting seat, temperature sensor, humidity transducer and alarm lamp, backup pad equidistance fixed connection lean on the last board placed in the installation box on the surface, the backup pad be provided with the culture dish standing groove on the surface, the connecting seat is rectangle array fixed connection and places the board in the middle of the installation box on the surface, the connecting seat outward appearance is cylindric, and this biological stem cell storage culture reagent case does not select for use elastic component, and stability in the use is good, can deposit multiunit cell sample simultaneously, excellent in use effect.
Above-mentioned device places the board through the multilayer in box and the box and places the culture dish, though can look over the culture dish at edge through the observation window that sets up, but when needs observe the culture dish that is close to the intermediate position in the box, then have to open movable door board and will place the board and shift out, at this in-process, the bacterium of external environment can cause the pollution to the culture dish on the one hand, on the other hand, when the environment of outside gets into inside the box, can break the environment of constant temperature and humidity in the box.
Therefore, it is necessary to provide a reagent kit for storing and culturing biological stem cells to solve the above technical problems.
Disclosure of Invention
The invention aims to provide a reagent box for storing and culturing biological stem cells, which aims to solve the problems that in the prior art, when a culture dish close to the middle position in a box body needs to be observed, a movable door plate has to be opened to move a placing plate out, bacteria in the external environment pollute the culture dish, and the constant-temperature and constant-humidity environment in the box body is broken when the external environment enters the box body.
Based on the above thought, the invention provides the following technical scheme: a reagent box for storing and culturing biological stem cells comprises a box body and a partition plate arranged in the box body in a sliding mode, wherein the top of the partition plate is fixedly connected with a plurality of storage frames, and one side of the top of each storage frame is hinged with a sealing plate;
the sealing plate is connected with the storage frame through the limiting assembly, the top pressure assembly is assembled on the limiting assembly, a through groove is formed in one side of the box body in a penetrating mode, one end of the partition plate penetrates through the through groove and is fixedly connected with a pulling plate, when the sealing plate covers the top of the storage frame, the limiting assembly locks the sealing plate and the storage frame, the partition plate is pulled out of the box body through the pulling plate in the process, and the top pressure assembly exerts pressure on the sealing plate to enable the sealing plate to be in close contact with the storage frame.
As a further scheme of the invention: the limiting assembly comprises a limiting plate which is fixedly connected with the sealing plate and is L-shaped in cross section, a groove matched with the limiting plate is formed in the top surface of the storage frame, a limiting block is connected in the groove in a sliding mode, one side of the top of the limiting block is arranged to be an inclined plane, a strip-shaped groove is formed in the side wall, close to the sealing plate, of the inner cavity of the groove, the limiting block is arranged in the strip-shaped groove in a sliding mode, a third spring is arranged at one end, located in the limiting block, of the strip-shaped groove, a guide groove is formed in the partition plate, an ejector rod is arranged in the guide groove in a sliding mode, a pull rope is fixedly arranged between the ejector rod and the limiting block, a first spring is arranged at one end inside the guide groove, and the two ends of the first spring are fixed to the end faces of the ejector rod and the guide groove respectively.
As a further scheme of the invention: the roof pressure subassembly includes spacing axle, spacing axle passes the limiting plate and rather than threaded connection, the recess internal rotation is connected with the pivot, and the bottom of pivot is passed and is stored the frame and extend to in the baffle, the top of pivot sets up in the recess, and the top end face of pivot has seted up the spacing groove, the one end that the closing plate was kept away from to spacing axle sets up the grafting portion to the platykurtic, grafting portion cooperatees with the epaxial spacing groove of pivot, the fixed cover in the outside of pivot is equipped with the gear, fixedly connected with and gear engaged with rack on the inner wall of box.
As a further scheme of the invention: the end face, close to the sealing plate, of the limiting shaft is provided with a round hole, a pressing block is arranged in the round hole in a sliding mode, a fourth spring is arranged in the round hole, and two ends of the fourth spring are fixed with the pressing block and one end face, opposite to the round hole, of the fourth spring respectively.
As a further scheme of the invention: the inner side surface of the sealing plate is fixedly connected with an arc-shaped supporting rod, a sliding groove matched with the supporting rod for use is formed in the storage frame, the supporting rod extends into the sliding groove and is in sliding fit with the storage frame through the sliding groove, a second spring is arranged at the bottom end inside the sliding groove, and the circle centers of the supporting rod and the sliding groove are coincided with the rotation center of the sealing plate.
As a further scheme of the invention: the inside fixedly connected with of box carries out the backup pad that supports to the baffle, backup pad and baffle sliding fit.
As a further scheme of the invention: the outer side peripheral surface of the ejector rod is provided with a rope groove, and one end of the pull rope, far away from the limiting block, is fixed with the inner bottom surface of the rope groove.
As a further scheme of the invention: the inner wall of the box body is fixedly connected with a lug which is matched with the sealing plate.
As a further scheme of the invention: the inner wall of the through groove is fixedly provided with a sealing gasket which is made of rubber materials.
As a further scheme of the invention: the both sides of arm-tie all rotate and are connected with the pull rod, fixedly connected with and the couple of pull rod looks block on the box lateral wall.
Compared with the prior art, the invention has the beneficial effects that: when the closing plate lid fits when storing the frame top, the closing plate is in the horizontality, and the closing plate equals with the height that leads to the groove with the height that stores the frame stack together this moment, and then be convenient for will store frame and closing plate and pull out, extrude the closing plate through the chamfer that leads to the groove and make its gradual downwardly rotating and laminate mutually with storing the frame, after closing plate and the laminating of storing the frame, can lock the closing plate through spacing subassembly, make its stable lid fit on storing the frame, and at the in-process that pulls out baffle and storage frame through the arm-tie, exert pressure to the closing plate through the roof pressure subassembly, make the closing plate with store between the frame contact inseparable, thereby avoid outside air to get into to cause the pollution to the culture dish in the holding vessel.
Drawings
The invention is further illustrated with reference to the following figures and examples:
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the case of the present invention;
FIG. 3 is a schematic view of the storage frame, sealing plate and partition plate of the present invention;
FIG. 4 is a schematic view of the structure of the pulling plate and the pulling rod of the present invention;
FIG. 5 is an enlarged view of the structure at A in FIG. 3 according to the present invention;
FIG. 6 is an enlarged view of the structure at B in FIG. 3 according to the present invention;
FIG. 7 is a cross-sectional view of the storage frame, partition and sealing plate of the present invention;
FIG. 8 is an enlarged view of FIG. 7 at C according to the present invention;
FIG. 9 is a schematic view of the compact of the present invention;
fig. 10 is a schematic view of the push rod and pull rope structure of the present invention.
In the figure: 1. a box body; 2. a pull rod; 3. pulling a plate; 4. a storage frame; 5. a partition plate; 6. sealing plates; 7. a rack; 8. a through groove; 9. a gear; 10. a support bar; 11. a limiting plate; 12. a storage tank; 13. a groove; 14. a top rod; 15. a notch; 16. an air bag; 17. a limiting shaft; 18. a plug-in part; 19. a rotating shaft; 20. a support plate; 21. a first spring; 22. a bump; 23. a second spring; 24. a chute; 25. a third spring; 26. pulling a rope; 27. a fourth spring; 28. briquetting; 29. rope grooves; 30. a limiting groove; 31. and a limiting block.
Description of the preferred embodiment
As shown in fig. 1-2, a biological stem cell storage culture reagent case, including the box 1 that is used for placing the culture dish, and slide in the box 1 and be provided with baffle 5, a plurality of storage frames 4 of the top fixedly connected with of baffle 5, a plurality of storage frames 4 set up of laminating each other, and 1 one side of box is the opening, the opening part articulates there is the door plant to in sealing up box 1, keep the constant temperature and humidity environment in the box 1, during the in-service use, set up storage tank 12 at the top of storage frame 4, place the culture dish in storage tank 12 and close the door plant.
In the practical application process, can install temperature sensor and humidity transducer in box 1, thereby be convenient for detect the temperature and humidity in the box 1, temperature sensor and humidity transducer's output all is connected with a controller, for example PCL or singlechip, carry out analysis and processing and transmit to outside display terminal through the information that the controller was gathered temperature sensor and humidity transducer, thereby be convenient for the staff knows the cultivation environment in the box 1 in real time, thereby intervene it, of course, the concrete structure and the circuit connection etc. of above-mentioned device are ripe technical means, do not do here and describe repeatedly.
As shown in fig. 2-8 and 10, a sealing plate 6 is hinged to one side of the top of the storage frame 4, the sealing plate 6 is connected with the storage frame 4 through a limiting component, a jacking component is assembled on the limiting component, a through groove 8 is formed in one side of the box body 1, so that one end of the partition plate 5 penetrates through the through groove 8 and is in sliding fit with the box body 1, the bottom surface of the partition plate 5 is flush with the inner bottom surface of the through groove 8, the partition plate 5 can smoothly slide out of the box body 1, a pulling plate 3 is arranged at the position, located in the through groove 8, of the side surface of the box body 1, and one end, penetrating out of the through groove 8, of the partition plate 5 is fixedly connected with the pulling plate 3.
In the actual use process, when the culture dish in the box body 1 needs to be checked, the partition plate 5 can be pulled outwards through the pull plate 3, the storage frame 4 at the top of the partition plate 5 can be driven to move outwards synchronously through the partition plate 5, and when the storage frame 4 moves to the through groove 8 of the box body 1, the sealing plate 6 is extruded by the side wall of the box body 1, so that the sealing plate 6 can rotate towards the direction of the storage frame 4 and finally covers the top of the storage frame 4 to seal the storage groove 12, specifically, when the sealing plate 6 covers the top of the storage frame 4, the sealing plate 6 is in a horizontal state, and the height of the sealing plate 6 and the storage frame 4 which are overlapped together is equal to the height of the through groove 8, so that the storage frame 4 and the sealing plate 6 can be pulled out conveniently, and in practical application, set up a chamfer on leading to the edge of groove 8 inboard side, be favorable to extrudeing closing plate 6, make closing plate 6 be being close to the in-process of box 1 lateral wall gradually, extrude closing plate 6 through the chamfer on leading to the groove 8 and make it rotate downwards gradually and laminate mutually with storage frame 4, after closing plate 6 and the laminating of storage frame 4, can lock closing plate 6 through spacing subassembly, make its stable lid fit on storage frame 4, and in-process pulling out baffle 5 and storage frame 4 through arm-tie 3, exert pressure to closing plate 6 through the roof pressure subassembly, make closing plate 6 and store between the frame 4 contact inseparable, thereby avoid outside air to get into and cause the pollution to the culture dish in the holding vessel 12.
Above-mentioned spacing subassembly includes the limiting plate 11 that is the L type with two cross sections of closing plate 6 fixed connection, two limiting plates 11 set up in closing plate 6 and are close to one side of storing frame 4, and both sides all seted up around the top surface of storing frame 4 with limiting plate 11 matched with recess 13, sliding connection has stopper 31 in recess 13, stopper 31 top one side sets up to the inclined plane, specifically, the bar groove has been seted up on the recess 13 inner chamber is close to the lateral wall of closing plate 6, and stopper 31 then slides and sets up in this bar inslot, furthermore, at least, be provided with a third spring 25 at the bar inslot, third spring 25's both ends respectively with stopper 31 and the relative side fixed connection in bar groove.
Further, it is provided with ejector pin 14 to slide in baffle 5, be provided with stay cord 26 between ejector pin 14 and the stopper 31, specifically, offer on the baffle 5 with 14 sliding fit's of ejector pin guide way, and the inside one end of guide way is provided with first spring 21, the both ends of first spring 21 are fixed mutually with the terminal surface of ejector pin 14 and guide way respectively, and the terminal surface fixed connection in the bar groove is arranged in to the one end of stay cord 26 and stopper 31, the other end of stay cord 26 passes and stores frame 4 and extend to in the baffle 5 with ejector pin 14 fixed connection.
The top pressure component comprises a limiting shaft 17, the limiting shaft 17 penetrates through the limiting plate 11 and is in threaded connection with the limiting plate, a rotating shaft 19 is connected to the groove 13 in a rotating mode, the bottom end of the rotating shaft 19 penetrates through the storage frame 4 and extends into the partition plate 5, the rotating shaft 19 is connected with the storage frame 4 and the partition plate 5 in a rotating mode, the top end of the rotating shaft 19 is arranged in the groove 13, a limiting groove 30 is formed in the end face of the top of the rotating shaft 19, a flat inserting portion 18 is arranged at one end, away from the sealing plate 6, of the limiting shaft 17, and the inserting portion 18 is matched with the limiting groove 30 in the rotating shaft 19.
Furthermore, a gear 9 is fixedly sleeved on the outer side of the rotating shaft 19, in order to facilitate the installation of the gear 9, a notch matched with the gear 9 is formed in the partition plate 5, so that the gear 9 is rotatably arranged in the notch, the outer edge of the gear 9 protrudes out of the notch, a rack 7 meshed with the gear 9 is fixedly connected to the inner wall of the box body 1, and the rack 7 is parallel to the partition plate 5.
When the storage box is in actual use, the partition plate 5 and the storage frame 4 are gradually pulled outwards through the pull plate 3, when the partition plate 5 slides outwards, the ejector rods 14 on the partition plate 5 gradually break away from contact with the inner wall of the box body 1, at the moment, the ejector rods 14 slide towards the outer side of the guide groove under the action of the first spring 21, and the pull rope 26 is loosened, at the moment, the limiting blocks 31 protrude outwards under the action of the third spring 25, when the sealing plate 6 gradually approaches the through groove 8, the sealing plate 6 is extruded through the side edges of the through groove 8, the sealing plate 6 gradually rotates towards the direction of the storage frame 4 and is attached to the storage frame 4, in the process that the sealing plate 6 is attached to the storage frame 4, the limiting plates 11 are gradually inserted into the grooves 13 and extrude the limiting blocks 31, because the tops of the limiting blocks 31 are inclined planes, therefore, the limiting blocks 31 are gradually extruded through the limiting plates 11 to overcome the action of the third springs 25 and are accommodated into the strip-shaped grooves, when the L-shaped limiting plates 11 pass through the limiting plates 31 and are staggered with the limiting plates 31, at the tops of the third springs 25, so that the sealing plates can limit the sealing plates 6 to limit the tops of the storage frame 6 to the storage groove 12, and prevent the storage frame 4 from being contaminated by air in the storage groove 12, and the storage container 12;
when the limiting plate 11 passes over the limiting block 31, the limiting shaft 17 is arranged between the limiting block 31 and the rotating shaft 19, and the inserting portion 18 on the limiting shaft 17 is arranged in the limiting groove 30 of the rotating shaft 19, so that the rotating shaft 19 can drive the limiting shaft 17 to rotate, at the moment, the baffle plate 5 and the storage frame 4 are continuously pulled outwards through the pulling plate 3, when the baffle plate 5 drives the gear 9 to move to the rack 7, the rack 7 is meshed with the gear 9 and gradually drives the gear 9 to rotate, the rotating shaft 19 can be driven to rotate through the gear 9, the rotating shaft 19 can synchronously drive the limiting shaft 17 to rotate by utilizing the matching of the inserting portion 18 and the limiting groove 30, because the limiting shaft 17 is in threaded engagement with the limiting plate 11, the limiting shaft 17 can gradually move upwards and is extruded with the limiting block 31 in the rotating process, so that the limiting shaft 17 is compressed between the limiting block 31 and the limiting plate 11, and the sealing plate 6 and the storage frame 4 are tightly arranged by forming opposite pressure, which is beneficial to further improving the sealing property between the sealing plate 6 and the storage frame 4, and the sealing property of observing the storage frame 4 by adopting transparent materials such as glass.
In summary, in the device, the sealing plate 6 and the limiting component are arranged, so that when the storage frame 4 is pulled out, the sealing plate 6 rotates downwards and is attached to the top of the storage frame 4, and the sealing plate 6 and the storage frame 4 are locked by the limiting component, so that the culture dish is sealed in the storage groove 12, and meanwhile, the sealing plate 6 and the storage frame 4 are closely attached by the arranged jacking component, so that the sealing performance between the sealing plate 6 and the storage frame 4 is further improved.
As shown in fig. 7 and 9, because the friction between the limiting plate 11 and the limiting block 31 causes the connection between the limiting plate 11 and the limiting block 31 to become loose gradually along with the increase of the service time, in this solution, the requirement of the close fit between the sealing plate 6 and the storage frame 4 is further met by the further fit between the limiting shaft 17 and the limiting block 31, but in order to avoid the problem that the partition 5 is difficult to be drawn out due to the excessive contact between the limiting shaft 17 and the limiting block 31, a circular hole is opened on the end surface of the limiting shaft 17 close to the sealing plate 6, a pressing block 28 is slidably disposed in the circular hole, in addition, a fourth spring 27 is further disposed in the circular hole, so that two ends of the fourth spring 27 are respectively fixed with the pressing block 28 and one end surface opposite to the circular hole, when the limiting shaft 17 is driven to rotate by the rotating shaft 19, the rotating shaft 19 drives the pressing block 28 to be extruded with the limiting block 31, and the pressing block 28 overcomes the pressure of the fourth spring 27 to be contracted inward of the circular hole, thereby avoiding the problem that the partition 5 is difficult to be drawn out due to the excessive extrusion between the limiting shaft 17 and the limiting block 31.
As shown in fig. 7, in actual installation, mounting grooves communicated with each other are formed on the inner side surface of the sealing plate 6 and the top surface of the storage frame 4, hinges are arranged in the mounting grooves, so that the sealing plate 6 is hinged to the storage frame 4 through the hinges and can cover the top of the storage frame 4, and in order to enable the sealing plate 6 to be reset, an arc-shaped support rod 10 is fixedly connected to the inner side surface of the sealing plate 6, a chute 24 matched with the support rod 10 is formed in the storage frame 4, the chute 24 is also set to be pasty, so that the support rod 10 extends into the chute 24 and is in sliding fit with the storage frame 4 through the chute 24, a second spring 23 is arranged at the bottom end inside the chute 24, two ends of the second spring 23 are fixed to the end surfaces opposite to the support rod 10 and the chute 24, and the centers of circles of the support rod 10 and the chute 24 coincide with the rotation center of the sealing plate 6.
During the in-service use, when needs reinsert baffle 5 to box 1 inside, can push baffle 5 towards box 1 inside through arm-tie 3, along with the extrusion gradually of ejector pin 14 and box 1 inner wall, make ejector pin 14 accomodate to the guide way in, stay cord 26 is pull this moment, thereby stimulate stopper 31 to the bar inslot and stagger with spacing axle 17, can make bracing piece 10 and closing plate 6 outwards rotate to vertical form under the effort of second spring 23 this moment, thereby open hold up tank 12, the culture dish exposes in box 1 this moment, and the width of baffle 5 will be less than the width of box 1, make the air of box 1 can be evenly to the culture dish contact of upper and lower floor.
As shown in fig. 1, a handle is fixedly connected to the outer side of the pulling plate 3, the pulling rod 2 is rotatably connected to both sides of the pulling plate 3, and a hook engaged with the pulling rod 2 is fixedly connected to the side wall of the box body 1, so that in actual use, when the partition board 5 is pushed into the box body 1 through the pulling plate 3, the pulling rod 2 is rotated to be engaged with the hook, and the pulling plate 3 is fixed.
And a supporting plate 20 for supporting the partition plate 5 is fixedly connected inside the box body 1, and the supporting plate 20 is in sliding fit with the partition plate 5.
As shown in fig. 4, two side walls inside the through groove 8 are both provided with notches 15, and an airbag 16 is fixedly arranged at the notches 15, so that the gear 9 can be prevented from interfering with the through groove 8 in the process that the partition plate 5 slides out of the box body 1 through the arranged notches 15, and the airbag 16 is favorable for being attached to the partition plate 5 and the side surface of the storage frame 4, thereby achieving the sealing effect and preventing external gas from entering the box body 1 through the through groove 8;
meanwhile, can be at the sealed pad of fixed setting by the rubber materials preparation on the inner wall that leads to groove 8, when closing plate 6 lid fits and stores frame 4 top and take baffle 5 out through arm-tie 3, baffle 5, store frame 4 and closing plate 6 and enclose the whole of closing and will laminate mutually with this sealed pad, thereby make the in-process of observing the culture dish, outside air is difficult to get into to 1 inside of box through leading to groove 8, be favorable to keeping 1 internal environment's of box stability, therefore, this scheme not only can avoid causing the pollution to the culture dish at the in-process of observing, also can avoid the outside air to cause the pollution to the environment in the box 1.
As shown in fig. 7, a bump 22 is further fixedly connected to the inner wall of the box body 1, when the partition 5 drives the sealing plate 6 to move toward the through groove 8, the sealing plate 6 contacts the bump 22 first, and the sealing plate 6 can be urged to rotate toward the storage frame 4 by pressing the bump 22 against the sealing plate 6, thereby facilitating stable extraction of the partition 5 and the storage frame 4.
As shown in fig. 7 and 10, a rope groove 29 is formed in the outer circumferential surface of the ejector rod 14, and one end of the pulling rope 26, which is far away from the limiting block 31, is fixed to the inner bottom surface of the rope groove 29, so that the pulling rope 26 is prevented from being clamped between the ejector rod 14 and the partition plate 5 in the moving process of the ejector rod 14.
Claims (10)
1. The utility model provides a biological stem cell storage culture reagent case, includes that box and slip set up the baffle in the box, the top fixedly connected with of baffle stores the frame, its characterized in that in a plurality of: a sealing plate is hinged to one side of the top of the storage frame;
the utility model discloses a storage box, including box, closing plate, storage frame, baffle, roof pressure subassembly, closing plate, storage frame, baffle one end, baffle lid, roof pressure subassembly, baffle and storage frame, the closing plate is connected through spacing subassembly with storing between the frame, be equipped with the roof pressure subassembly on the spacing subassembly, the logical groove that runs through is seted up to box one side, baffle one end is passed logical groove and fixedly connected with arm-tie, and when the closing plate lid fits the storage frame top, spacing subassembly locks closing plate and storage frame, and pulls out the in-process of box with the baffle through the arm-tie, the roof pressure subassembly exerts pressure to the closing plate for closing plate and storage frame in close contact with.
2. The reagent box for storing and culturing the biological stem cells as claimed in claim 1, wherein: the limiting assembly comprises a limiting plate which is fixedly connected with the sealing plate and is L-shaped in cross section, a groove matched with the limiting plate is formed in the top surface of the storage frame, a limiting block is connected in the groove in a sliding mode, one side of the top of the limiting block is arranged to be an inclined plane, a strip-shaped groove is formed in the side wall, close to the sealing plate, of the inner cavity of the groove, the limiting block is arranged in the strip-shaped groove in a sliding mode, a third spring is arranged at one end, located in the limiting block, of the strip-shaped groove, a guide groove is formed in the partition plate, an ejector rod is arranged in the guide groove in a sliding mode, a pull rope is fixedly arranged between the ejector rod and the limiting block, a first spring is arranged at one end inside the guide groove, and the two ends of the first spring are fixed to the end faces of the ejector rod and the guide groove respectively.
3. The reagent box for storing and culturing the biological stem cells as claimed in claim 2, wherein: the roof pressure subassembly includes spacing axle, spacing axle passes the limiting plate and rather than threaded connection, the recess internal rotation is connected with the pivot, and the bottom of pivot is passed and is stored the frame and extend to in the baffle, the top of pivot sets up in the recess, and the top end face of pivot has seted up the spacing groove, the one end that the closing plate was kept away from to spacing axle sets up the grafting portion to the platykurtic, grafting portion cooperatees with the epaxial spacing groove of pivot, the fixed cover in the outside of pivot is equipped with the gear, fixedly connected with and gear engaged with rack on the inner wall of box.
4. The reagent box for storing and culturing the biological stem cells as claimed in claim 3, wherein: the limiting shaft is provided with a round hole on the end face, close to the sealing plate, of one end, a pressing block is arranged in the round hole in a sliding mode, a fourth spring is arranged in the round hole, and the two ends of the fourth spring are fixed with the pressing block and one end face, opposite to the round hole, of the pressing block respectively.
5. The reagent box for storing and culturing the biological stem cells as claimed in claim 1, wherein: the inner side surface of the sealing plate is fixedly connected with an arc-shaped supporting rod, a sliding groove matched with the supporting rod for use is formed in the storage frame, the supporting rod extends into the sliding groove and is in sliding fit with the storage frame through the sliding groove, a second spring is arranged at the bottom end inside the sliding groove, and the circle centers of the supporting rod and the sliding groove are coincided with the rotation center of the sealing plate.
6. The reagent box for storing and culturing the biological stem cells as claimed in claim 1, wherein: the inside fixedly connected with of box carries out the backup pad that supports to the baffle, backup pad and baffle sliding fit.
7. The reagent box for storing and culturing the biological stem cells as claimed in claim 2, wherein: the outer side peripheral surface of the ejector rod is provided with a rope groove, and one end of the pull rope, far away from the limiting block, is fixed with the inner bottom surface of the rope groove.
8. The reagent box for storing and culturing the biological stem cells as claimed in claim 1, wherein: the inner wall of the box body is fixedly connected with a lug which is matched with the sealing plate.
9. The reagent box for storing and culturing the biological stem cells as claimed in claim 1, wherein: and a sealing gasket is fixedly arranged on the inner wall of the through groove and is made of a rubber material.
10. The reagent box for storing and culturing the biological stem cells as claimed in claim 2, wherein: the both sides of arm-tie all rotate and are connected with the pull rod, fixedly connected with and the couple of pull rod looks block on the box lateral wall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310046421.7A CN115786117B (en) | 2023-01-31 | 2023-01-31 | Biological stem cell storage culture reagent case |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116040115A (en) * | 2023-04-03 | 2023-05-02 | 成都大学 | Visual save set that seed ultralow temperature preserved usefulness |
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