CN112918867B

CN112918867B - DNA extraction kit

Info

Publication number: CN112918867B
Application number: CN202110223119.5A
Authority: CN
Inventors: 李志航; 郜敏伟
Original assignee: Zhuhai Baorui Biotechnology Co ltd
Current assignee: Zhuhai Baorui Biotechnology Co ltd
Priority date: 2021-03-01
Filing date: 2021-03-01
Publication date: 2022-06-03
Anticipated expiration: 2041-03-01
Also published as: CN112918867A

Abstract

The invention relates to the field of kits, in particular to a DNA extraction kit, which comprises a kit body, a rotating structure, a fixing structure, a clamping structure, a freezing structure, a drawing structure and a moving structure; through the internally mounted rotating-structure at the box body, not only can play sealed protection's effect to the box body, can also guarantee to place and be in vertical state all the time in the inside test tube of box body, thereby can avoid when transporting the box body, the condition of weeping appears in the test tube, greatly increased the transportation security of reagent, and the cooperation is installed in the inside freezing structure of box body, not only can play cold-stored effect to placing in the inside reagent of box body, can also concentrate the collection processing to the outside condensation drop of water, and can also realize quick installation and dismantlement, convenient operation, the rotating-structure's through placing the inside sets up fixed knot structure, can lock the outside lateral wall of male test tube and fix, avoid when transporting reagent, the condition that the test tube takes place to rock, prevent the weeping.

Description

DNA extraction kit

Technical Field

The invention relates to the field of kits, in particular to a DNA extraction kit.

Background

DeoxyriboNucleic Acid (abbreviated as DNA) is one of the four kinds of nucleic acids contained in biological cells. DNA carries the genetic information necessary for the synthesis of RNA and proteins, and is a biological macromolecule essential for the development and proper functioning of an organism. DNA is a macromolecular polymer composed of deoxynucleotides. Deoxynucleotides are composed of bases, deoxyribose, and phosphate. Wherein, the basic groups are 4 types: adenine (a), guanine (G), thymine (T) and cytosine (C). In the structure of a DNA molecule, two polydeoxyribonucleotide chains are coiled around a common central axis to form a double helix structure. The deoxyribose-phosphate chain is outside the helix, with the bases facing inward. The two polydeoxyribonucleotide strands are complementary in opposite directions and are linked by base pairing via hydrogen bonds between the bases to form a relatively stable combination. The kit is a box for containing chemical reagents for detecting chemical components, drug residues, virus species and the like. The DNA extraction kit is a storage box for containing DNA extraction reagents.

At present, a general DNA extraction kit is simple in structure and basically comprises a box body and a test tube groove for placing test tubes, when the kit needs to be transported, the kit can incline, the test tubes placed in the kit can be in an inclined state at the moment, liquid leakage is easy to occur, and because the inner diameter of the general test tube groove is larger than the outer diameter of the test tubes, when the kit is transported, if jolting occurs, the test tubes can shake violently, so that the transportation safety of reagents is seriously influenced, and because the general reagents need to be refrigerated, when the kit is transported for a long distance, the temperature in the kit can be higher, so that the transportation quality of the reagents is seriously influenced, if ice bags are placed, water drops generated on the ice bags are not easy to collect and discharge, and the ice bags are inconvenient to replace and carry out sealed heat preservation, inconvenient pulling box body when not needing to remove, sets up the gyro wheel on the box body and is inconvenient to be accomodate, just probably has the condition of placing unstability, seriously influences the use of reagent.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides a DNA extraction kit.

The technical scheme adopted by the invention for solving the technical problems is as follows: a DNA extraction kit comprises a box body, a rotating structure, a fixing structure, a clamping structure, a freezing structure, a drawing structure and a moving structure, wherein the rotating structure which is used for sealing the box body and can ensure that a test tube is always kept in a vertical state to prevent liquid leakage is arranged at one end of the box body which is used for storing the test tube, and the fixing structure which can lock and fix the test tube to prevent the test tube from shaking is arranged in the rotating structure; the clamping structure capable of clamping and fixing the test tube is arranged in the rotating structure, and the freezing structure capable of cooling and freezing the reagent in the box body is arranged in the box body; the bottom of box body is inside to be set up and to carry out the convenient pulling of pull the pull structure the bottom internally mounted of box body is convenient the box body removes remove the structure.

Specifically, revolution mechanic includes bearing, pivot, places cover, standing groove, case lid, sealing washer, hinge and weight piece the both ends lateral wall of box body is fixed one respectively the bearing is two the inside of bearing is all fixed one the pivot is two it is fixed between the pivot place the cover, just place the cover and pass through the pivot with the bearing with the box body rotates to be connected place the inside of cover is equipped with a plurality ofly and is latticed structure the standing groove the one end of box body is passed through the hinge rotates to be connected the case lid the bottom of case lid is glued the sealing washer place the bottom mounting of cover the weight piece.

Specifically, the fixed knot constructs including first guide arm, fixed plate, block rubber and first spring, every the both sides of standing groove all fix one first guide arm the outside of first guide arm slides the fixed plate, just the fixed plate with place the cover and slide the glued block rubber of one end of fixed plate, just the cross-section of block rubber is trapezium structure the fixed plate with the centre gripping is fixed between the box body first spring.

Specifically, the centre gripping structure includes dwang, second guide arm, splint, rubber pad and second spring, every the both sides of standing groove are all fixed one the second guide arm the outside of second guide arm is slided splint, just splint with place the cover and slide splint with it is fixed to place the centre gripping between the cover the second spring the inside of box body is rotated the dwang, just contradict respectively at the both ends of dwang splint with the fixed plate.

Specifically, the freezing structure comprises a sealing cover, an ice bag, air outlet holes, a filter screen, two water storage tanks, a sealing plug, an inserting rod, a third spring, an inserting slot and a button, wherein one water storage tank is arranged inside each of two ends of the box body, the two water storage tanks are communicated, one filter screen is fixed inside each of the two water storage tanks, the ice bag is arranged inside each water storage tank, the air outlet holes which are inclined are arranged on the side walls of the two ends inside the box body, the sealing plug is arranged at one end of the box body, the sealing cover is arranged at the top end of each water storage tank, the inserting rod slides inside the sealing cover, the third spring is clamped and fixed between the inserting rod and the sealing cover, one end of the inserting rod, which deviates from the third spring, is of a trapezoidal structure, the inserting slot with a cross-shaped structure is arranged inside the box body, and the button in a cross-shaped structure slides in the slot.

Specifically, the drawing structure comprises a stretching rod, a handle, a pull rod, limiting holes, a limiting rod and a fourth spring, the bottom sliding section of the box body is of a T-shaped structure, one end of the stretching rod is fixed to the handle, the stretching rod is provided with two limiting holes, the internal sliding section of the box body is of a T-shaped structure, the limiting rod and the box body are clamped and fixed through the fourth spring, the limiting rod can be inserted into the limiting holes, one end of the limiting rod is fixed to the pull rod in a perpendicular relation, and the pull rod and the box body slide.

Specifically, the moving structure comprises a fixed column, two mounting rods, a roller, a gear, a rack, a slider and a fifth spring, the fixed column is rotated inside the bottom end of the box body, the two mounting rods are fixed outside the fixed column, one end of each mounting rod is rotated to enable the roller to be accommodated inside the box body, the gear is fixed outside the fixed column and enabled to rotate with the box body, the rack slides inside the box body and is meshed with the gear, the rack can abut against the stretching rod, the slider is fixed at one end of the rack in a vertical relation, and the fifth spring is clamped and fixed between the slider and the box body.

The invention has the beneficial effects that:

(1) the DNA extraction kit not only can play a role in sealing and protecting the box body, but also can ensure that the test tube placed in the box body is always in a vertical state by installing the rotating structure in the box body, thereby avoiding the situation of liquid leakage of the test tube when the box body is transported, greatly improving the transportation safety of the reagent, and the DNA extraction kit is matched with the freezing structure arranged in the box body, not only can play a role in refrigerating the reagent placed in the box body, but also can intensively collect and treat externally condensed water drops, can realize quick installation and disassembly, is convenient to operate, only needs to open the box cover when in use, then inserts the test tube containing the reagent into the placing groove in the placing sleeve in sequence, and because the placing sleeve is rotatably connected with the box body through the rotating shaft and the bearing, and because the weighting block is installed at the bottom end of the placing sleeve, thereby ensuring that no matter how the box body inclines, the test tube placed in the placing groove is in a vertical state, thereby effectively avoiding the condition of reagent leakage caused by the inclination of the box body during transportation, greatly improving the transportation safety, the two ends of the box body are respectively provided with a water storage groove, the interior of the water storage groove can be used for placing ice bags, the refrigeration effect on the reagent in the test tube can be achieved through the two ice bags in a symmetrical relation, the ice bags can be supported through fixing a filter screen in the water storage groove, thereby the condensed water drops outside the ice bags can be intensively collected and discharged, the drainage is convenient, the sealing cover arranged at the top end of the water storage groove can achieve the sealing and heat preservation effects on the ice bags, because the inserting rod slides through a third spring in the sealing cover, and because one end of the inserting rod is in a trapezoidal structure, the installation of the sealing cover can not be influenced, because the inside at the box body is equipped with the slot, after the inserted bar inserted the inside of slot, sealed lid will realize installing fast fixed this moment, and through the setting of button, can also conveniently dismantle, conveniently install and dismantle convenient operation to the ice bag promptly.

(2) The DNA extraction kit can lock and fix the outer side wall of the inserted test tube by arranging the fixing structure in the rotating structure for placing the test tube, avoid the situation that the test tube shakes during reagent transportation, prevent liquid leakage, and can also act on the clamping structure in the fixing structure, and further clamp and fix the outer side wall of the test tube by driving the clamping structure to act through the fixing structure, so that the fixing stability of the test tube can be further improved, the safety of test tube transportation is ensured, namely when the test tubes containing the reagents are sequentially inserted into the placing grooves, as the rubber blocks with trapezoidal cross sections are all slid on the inner side wall of each placing groove, the rubber blocks can be slid towards the inside of the placing sleeve by the extrusion force of the downward sliding of the test tubes on the rubber blocks, as the fixing plate is fixed at one end of the rubber blocks, at the fixed plate with place the fixed first spring of centre gripping between the cover, the spring action at first spring, can make the block rubber conflict all the time in the outside lateral wall of test tube, just can realize fixed to the locking of test tube outside lateral wall, effectively prevent that the test tube from appearing the condition of rocking when the transportation, avoid the weeping, because the inside rotation dwang of placing the cover, and the both ends of dwang are contradicted with fixed plate and splint respectively, when the fixed plate slides and extrudes the dwang and rotate, splint that will contradict will be contradicted to the central point of standing groove to the other end of dwang puts and slides, because the one end at splint fixed rubber pad, the lateral wall of test tube will be contradicted to the rubber pad this moment, thereby realize that the further centre gripping of test tube is fixed, make the stability of placing of test tube higher.

(3) The DNA extraction kit can not only conveniently pull the box body, but also conveniently store the pulling assembly into the box body for locking and fixing when the pulling assembly does not need to move so as to effectively avoid space occupation, and the pulling structure can also act on the moving structure to enable the moving structure to rotate out or store so as to avoid moving when the pulling assembly needs to be placed, can conveniently rotate out and move when the pulling assembly needs to be moved, and is simple and convenient to operate, because the stretching rod with the T-shaped structure in the inner sliding section at the bottom end of the box body can be conveniently pulled out to pull the box body and also can be stored, space occupation when the pulling assembly does not need to be used is avoided, and because two limiting holes are arranged on the stretching rod and are matched with the limiting rods sliding in the box body, just can realize tensile pole spacing fixed, prevent that tensile pole from taking place the condition of self-sliding when pulling out and accomodating, and when tensile pole pulls out, the rack no longer receives tensile pole's conflict power this moment, the rack will take place to slide under the elastic force of fifth spring, the rack will drive the gear and take place clockwise rotation this moment, just can will fix the outside installation pole and the gyro wheel of fixed column and roll out, just can conveniently carry out the removal of box body this moment, when tensile pole is accomodate, will extrude the rack and slide, the gear takes place the antiport this moment, just can accomodate the inside of advancing the box body once more with installation pole and gyro wheel, thereby make things convenient for the stationarity of placing of box body.

Drawings

The invention is further illustrated with reference to the following figures and examples.

FIG. 1 is a schematic structural diagram of the overall structure of a preferred embodiment of the DNA extraction kit provided by the present invention;

FIG. 2 is a schematic view showing a coupling structure of the cartridge and the rotation structure shown in FIG. 1;

FIG. 3 is an enlarged view of portion A of FIG. 2;

FIG. 4 is an enlarged view of the portion B shown in FIG. 2;

FIG. 5 is a schematic view of the connection between the case and the freezing mechanism shown in FIG. 1;

FIG. 6 is an enlarged view of the portion C shown in FIG. 5;

FIG. 7 is an enlarged view of the portion D shown in FIG. 5;

fig. 8 is an enlarged view of the portion E shown in fig. 5.

In the figure: 1. a box body, 2, a rotating structure, 21, a bearing, 22, a rotating shaft, 23, a placing sleeve, 24, a placing groove, 25, a box cover, 26, a sealing ring, 27, a hinge, 28, a weighting block, 3, a fixing structure, 31, a first guide rod, 32, a fixing plate, 33, a rubber block, 34, a first spring, 4, a clamping structure, 41, a rotating rod, 42, a second guide rod, 43, a clamping plate, 44, a rubber pad, 45, a second spring, 5, a freezing structure, 51, a sealing cover, 52, an ice bag, 53, an air outlet hole, 54, a filter screen, 55, a water storage groove, 56, a sealing plug, 57, an insertion rod, 58, a third spring, 59, a slot, 59a button, 6, a drawing structure, 61, a stretching rod, 62, a handle, 63, a pull rod, 64, a limiting hole, 65, a limiting rod, 66, a fourth spring, 7, a moving structure, 71, a fixing column, 72, a mounting rod, 73 and a roller, 74. gear 75, rack 76, slider 77, fifth spring.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1-8, the DNA extraction kit of the present invention comprises a box body 1, a rotating structure 2, a fixing structure 3, a clamping structure 4, a freezing structure 5, a pulling structure 6 and a moving structure 7, wherein the rotating structure 2 for sealing the box body 1 and ensuring that the test tube is always kept in a vertical state to prevent leakage is installed at one end of the box body 1 for storing the test tube, and the fixing structure 3 for locking and fixing the test tube to prevent the test tube from shaking is arranged inside the rotating structure 2; the clamping structure 4 capable of clamping and fixing the test tube is arranged in the rotating structure 2, and the freezing structure 5 capable of cooling and freezing the reagent in the box body 1 is arranged in the box body 1; the inside setting in bottom of box body 1 can carry out the convenient pulling of pull structure 6 the bottom internally mounted of box body 1 is convenient box body 1 removes removal structure 7.

Specifically, the rotating structure 2 includes a bearing 21, a rotating shaft 22, a placing sleeve 23, a placing groove 24, a box cover 25, a sealing ring 26, a hinge 27 and a weighting block 28, wherein one bearing 21 is fixed on each of the two end side walls of the box body 1, one rotating shaft 22 is fixed inside each of the two bearings 21, the placing sleeve 23 is fixed between the two rotating shafts 22, the placing sleeve 23 is rotatably connected with the box body 1 through the rotating shaft 22 and the bearing 21, a plurality of placing grooves 24 in a grid structure are arranged inside the placing sleeve 23, one end of the box body 1 is rotatably connected with the box cover 25 through the hinge 27, the sealing ring 26 is cemented to the bottom end of the box cover 25, the weighting block 28 is fixed to the bottom end of the placing sleeve 23, when in use, only the box cover 25 needs to be opened, and then test tubes containing reagents are sequentially inserted into the placing grooves 24 inside the placing sleeve 23, because the placing sleeve 23 is rotatably connected with the box body 1 through the rotating shaft 22 and the bearing 21, and the weighting block 28 is installed at the bottom end of the placing sleeve 23, the test tube placed in the placing groove 24 is in a vertical state no matter how the box body 1 inclines, so that the condition that the reagent is leaked due to the fact that the box body 1 inclines in the transportation process can be effectively avoided, and the transportation safety is greatly improved.

Specifically, the fixing structure 3 includes a first guide rod 31, a fixing plate 32, a rubber block 33 and a first spring 34, one first guide rod 31 is fixed on each of the two sides of the placement groove 24, the fixing plate 32 slides outside the first guide rod 31, the fixing plate 32 slides with the placement sleeve 23, the rubber block 33 is glued to one end of the fixing plate 32, the cross section of the rubber block 33 is in a trapezoidal structure, the first spring 34 is clamped and fixed between the fixing plate 32 and the box body 1, when the test tubes containing the reagents are sequentially inserted into the placement grooves 24, the rubber block 33 having a trapezoidal structure in cross section slides on each of the inner side walls of the placement grooves 24, and the rubber block 33 can slide into the placement sleeve 23 by the pressing force of the test tube sliding down on the rubber block 33, because the one end of block rubber 33 is fixed plate 32 with it is fixed to place the centre gripping between the cover 23 first spring 34 the elastic action of first spring 34 can make block rubber 33 contradicts in the outside lateral wall of test tube all the time, just can realize fixed to the locking of the outside lateral wall of test tube, effectively prevents that the condition that the test tube rocked from appearing when the transportation, avoids the weeping.

Specifically, the clamping structure 4 includes a rotating rod 41, a second guide rod 42, a clamping plate 43, a rubber pad 44 and a second spring 45, one of the second guide rod 42 is fixed on each of the two sides of the placement groove 24, the clamping plate 43 slides on the outside of the second guide rod 42, and the clamping plate 43 slides on the placement sleeve 23, the clamping plate 43 and the placement sleeve 23 are clamped and fixed by the second spring 45, the rotating rod 41 rotates inside the box body 1, and the two ends of the rotating rod 41 respectively abut against the clamping plate 43 and the fixing plate 32, since the rotating rod 41 rotates inside the placement sleeve 23, and the two ends of the rotating rod 41 respectively abut against the fixing plate 32 and the clamping plate 43, when the fixing plate 32 slides and extrudes the rotating rod 41, the other end of the rotating rod 41 abuts against the clamping plate 43 to slide on the center position of the placement groove 24, because the rubber pad 44 is fixed to one end of the clamping plate 43, the rubber pad 44 will abut against the side wall of the test tube, so that the test tube is further clamped and fixed, and the placing stability of the test tube is higher.

Specifically, the freezing structure 5 includes a sealing cover 51, an ice bag 52, air outlets 53, a filter screen 54, water storage tanks 55, a sealing plug 56, an insertion rod 57, a third spring 58, a slot 59 and a button 59a, wherein one water storage tank 55 is arranged inside each of two ends of the box body 1, the two water storage tanks 55 are communicated, one filter screen 54 is fixed inside each of the two water storage tanks 55, the ice bag 52 is arranged inside the water storage tank 55, a plurality of inclined air outlets 53 are arranged on each of two side walls inside the box body 1, the sealing plug 56 is mounted at one end of the box body 1, the sealing cover 51 is arranged at the top end of the water storage tank 55, the insertion rod 57 is slid inside the sealing cover 51, the third spring 58 is clamped and fixed between the insertion rod 57 and the sealing cover 51, and one end of the insertion rod 57 departing from the third spring 58 is in a trapezoidal structure, the insertion groove 59 having a cross-shaped cross-section is provided in the box body 1, the button 59a having a cross-shaped cross-section is provided in the insertion groove 59 in a sliding manner, the water storage grooves 55 are provided in the two ends of the box body 1, the ice bag 52 can be placed in the water storage grooves 55, the two symmetrical ice bags 52 can provide a cold storage effect for reagents in a test tube, the filter net 54 is fixed in the water storage groove 55 to support the ice bag 52, so that condensed water drops outside the ice bag 52 can be collected and discharged in a concentrated manner, and drainage is facilitated, the sealing cover 51 provided at the top end of the water storage groove 55 can provide a sealing and heat-preserving effect for the ice bag 52, and the insertion rod 57 is provided in the sealing cover 51 in a sliding manner through the third spring 58, and because the one end of inserted bar 57 is the trapezium structure, can not influence the installation of sealed lid 51, because the inside of box body 1 is equipped with slot 59, work as inserted bar 57 inserts behind the inside of slot 59, sealed lid 51 will realize quick installation fixed this moment, and pass through button 59 a's setting, can also conveniently dismantle, it is convenient right promptly ice bag 52 is installed and is dismantled, convenient operation.

Specifically, the pulling structure 6 includes a stretching rod 61, a handle 62, a pulling rod 63, a limiting hole 64, a limiting rod 65 and a fourth spring 66, the stretching rod 61 with a T-shaped sliding section at the bottom end of the box body 1, the handle 62 fixed at one end of the stretching rod 61, the two limiting holes 64 arranged on the stretching rod 61, the limiting rod 65 with a T-shaped sliding section in the box body 1, the fourth spring 66 clamped and fixed between the limiting rod 65 and the box body 1, the limiting rod 65 capable of being inserted into the limiting hole 64, the pulling rod 63 fixed at one end of the limiting rod 65 in a perpendicular relationship, and the pulling rod 63 sliding with the box body 1, when needing to move, can be conveniently rotated out and moved, the operation is simple and convenient, because the stretching rod 61 with a T-shaped sliding section in the bottom end of the box body 1, therefore, the stretching rod 61 can be conveniently pulled out to pull the box body 1 and can be stored, so that space occupation is avoided when the stretching rod 61 is not needed, and the stretching rod 61 is provided with two limiting holes 64 matched with the limiting rods 65 sliding in the box body 1, so that the limiting fixation of the stretching rod 61 can be realized, and the condition that the stretching rod 61 slides automatically when being pulled out and stored is prevented.

Specifically, the moving mechanism 7 includes a fixed column 71, mounting rods 72, a roller 73, a gear 74, a rack 75, a slider 76, and a fifth spring 77, wherein the fixed column 71 is rotated inside the bottom end of the case 1, two of the mounting rods 72 are fixed to the outside of the fixed column 71, the roller 73 is rotated at one end of the mounting rods 72, the roller 73 can be received inside the case 1, the gear 74 is fixed to the outside of the fixed column 71, the gear 74 is rotated with the case 1, the rack 75 is slid inside the case 1, the rack 75 is engaged with the gear 74, the rack 75 can be abutted against the stretching rod 61, the slider 76 is fixed to one end of the rack 75 in a perpendicular relationship, the fifth spring 77 is held between the slider 76 and the case 1, and when the stretching rod 61 is pulled out, at this time, the rack 75 is no longer subjected to the contact force of the stretching rod 61, the rack 75 slides under the elastic force of the fifth spring 77, at this time, the rack 75 drives the gear 74 to rotate clockwise, the mounting rod 72 and the roller 73 fixed outside the fixed column 71 can be rotated out, at this time, the box body 1 can be conveniently moved, when the stretching rod 61 is stored, the rack 75 is extruded to slide, at this time, the gear 74 rotates reversely, the mounting rod 72 and the roller 73 can be stored in the box body 1 again, and therefore, the placing stability of the box body 1 is facilitated.

When in use, only the box cover 25 is needed to be opened, and then the test tubes containing the reagents are sequentially inserted into the placing groove 24 inside the placing sleeve 23, since the placing sleeve 23 is rotatably connected with the box body 1 through the rotating shaft 22 and the bearing 21, and since the weighting block 28 is installed at the bottom end of the placing sleeve 23, it can be ensured that the test tubes placed inside the placing groove 24 are in a vertical state no matter how the box body 1 inclines, thereby effectively avoiding the reagent leakage caused by the inclination of the box body 1 during transportation, greatly improving the transportation safety, the water storage grooves 55 are respectively arranged inside the two ends of the box body 1, the ice bags 52 can be placed inside the water storage grooves 55, and the effect of refrigerating the reagents inside the test tubes can be achieved through the two symmetrical ice bags 52, by fixing the filter net 54 inside the water storage tank 55, the ice pack 52 can be supported, thereby the condensed water drops outside the ice bag 52 can be collected and discharged in a centralized way, the water is convenient to drain, the sealing cover 51 arranged at the top end of the water storage tank 55 can play a role in sealing and heat preservation of the ice bag 52, since the insert rod 57 is slid inside the sealing cap 51 by the third spring 58, and since one end of the insertion rod 57 is a trapezoidal structure, the installation of the sealing cover 51 is not affected, since the insertion groove 59 is formed in the inner portion of the box body 1, when the insertion rod 57 is inserted into the insertion groove 59, the sealing cover 51 is quickly installed and fixed, the button 59a is arranged, so that the ice bag 52 can be conveniently detached, namely, the ice bag is conveniently mounted and detached, and the operation is convenient; when the test tubes containing the reagent are sequentially inserted into the placing groove 24, the rubber block 33 with the trapezoidal sliding cross section is arranged in the rubber block 33 of the placing groove 24, the rubber block 33 can slide towards the inside of the placing sleeve 23 through the extrusion force of the sliding of the test tubes to the rubber block 33, the fixing plate 32 is fixed at one end of the rubber block 33, the first spring 34 is clamped and fixed between the fixing plate 32 and the placing sleeve 23, the rubber block 33 can always abut against the outer side wall of the test tube under the elastic action of the first spring 34, the outer side wall of the test tube can be locked and fixed, the test tube is effectively prevented from shaking during transportation, liquid leakage is avoided, the rotating rod 41 rotates inside the placing sleeve 23, and the two ends of the rotating rod 41 abut against the fixing plate 32 and the clamping plate 43 respectively, when the fixed plate 32 slides and presses the rotating rod 41 to rotate, the other end of the rotating rod 41 will abut against the clamping plate 43 to slide towards the central position of the placing groove 24, and because the rubber pad 44 is fixed at one end of the clamping plate 43, the rubber pad 44 will abut against the side wall of the test tube, so that the test tube is further clamped and fixed, and the placing stability of the test tube is higher; when the stretching rod 61 with a T-shaped sliding section is arranged in the bottom end of the box body 1, the stretching rod 61 can be conveniently pulled out to pull the box body 1 and can be stored, so that the occupied space is avoided, the stretching rod 61 is provided with two limiting holes 64 which are matched with the limiting rod 65 sliding in the box body 1, the limiting and fixing of the stretching rod 61 can be realized, the stretching rod 61 is prevented from automatically sliding during pulling out and storing, when the stretching rod 61 is pulled out, the rack 75 does not bear the contact force of the stretching rod 61 any more, the rack 75 slides under the elastic force of the fifth spring 77, and the rack 75 drives the gear 74 to rotate clockwise, the mounting rod 72 and the roller 73 fixed to the outside of the fixing post 71 can be rotated out, so that the box body 1 can be conveniently moved, when the stretching rod 61 is accommodated, the rack 75 is pressed to slide, and the gear 74 rotates in the opposite direction, so that the mounting rod 72 and the roller 73 can be accommodated in the box body 1 again, and the placing stability of the box body 1 is facilitated.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the embodiments and descriptions given above are only illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A DNA extraction kit, which is characterized in that: the test tube box comprises a box body (1), a rotating structure (2), a fixing structure (3), a clamping structure (4), a freezing structure (5), a drawing structure (6) and a moving structure (7), wherein one end of the box body (1) used for storing test tubes is provided with the rotating structure (2) which is used for sealing the box body (1) and can ensure that the test tubes are always kept in a vertical state to prevent liquid leakage, and the fixing structure (3) which can lock and fix the test tubes to prevent the test tubes from shaking is arranged in the rotating structure (2); the clamping structure (4) capable of clamping and fixing the test tube is arranged in the rotating structure (2), and the freezing structure (5) capable of cooling and freezing the reagent in the box body (1) is arranged in the box body (1); the drawing structure (6) capable of being drawn and conveniently pulled is arranged in the bottom end of the box body (1), and the moving structure (7) capable of facilitating the movement of the box body (1) is arranged in the bottom end of the box body (1); the rotating structure (2) comprises a bearing (21), a rotating shaft (22), a placing sleeve (23), a placing groove (24), a box cover (25), a sealing ring (26), a hinge (27) and a weighting block (28), wherein the side walls of two ends of the box body (1) are respectively fixed with one bearing (21), the rotating shaft (22) is fixed in each of the two bearings (21), the placing sleeve (23) is fixed between the two rotating shafts (22), the placing sleeve (23) is rotatably connected with the box body (1) through the rotating shaft (22) and the bearing (21), the placing grooves (24) in a grid structure are arranged in the placing sleeve (23), one end of the box body (1) is rotatably connected with the box cover (25) through the hinge (27), and the sealing ring (26) is cemented at the bottom end of the box cover (25), fixing the weighting block (28) at the bottom end of the placing sleeve (23); the fixing structure (3) comprises first guide rods (31), fixing plates (32), rubber blocks (33) and first springs (34), wherein one first guide rod (31) is fixed on each of two sides of each placing groove (24), the fixing plates (32) are slid outside the first guide rods (31), the fixing plates (32) and the placing sleeves (23) are slid, the rubber blocks (33) are glued at one ends of the fixing plates (32), the cross sections of the rubber blocks (33) are in a trapezoid structure, and the first springs (34) are clamped and fixed between the fixing plates (32) and the box body (1); the clamping structure (4) comprises a rotating rod (41), second guide rods (42), clamping plates (43), rubber pads (44) and second springs (45), one second guide rod (42) is fixed on each of two sides of each placing groove (24), the clamping plates (43) slide outside the second guide rods (42), the clamping plates (43) slide with the placing sleeves (23), the second springs (45) are clamped and fixed between the clamping plates (43) and the placing sleeves (23), the rotating rod (41) is rotated inside the box body (1), and two ends of the rotating rod (41) respectively abut against the clamping plates (43) and the fixing plates (32); the freezing structure (5) comprises a sealing cover (51), an ice bag (52), air outlet holes (53), filter screens (54), water storage tanks (55), sealing plugs (56), insertion rods (57), a third spring (58), an insertion slot (59) and a button (59 a), wherein the two water storage tanks (55) are respectively arranged inside two ends of the box body (1), the two water storage tanks (55) are communicated, one filter screen (54) is fixed inside the two water storage tanks (55), the ice bag (52) is arranged inside the water storage tank (55), the air outlet holes (53) which are inclined are respectively arranged on the side walls of two ends inside the box body (1), the sealing plugs (56) are arranged at one end of the box body (1), the sealing covers (51) are arranged at the top ends of the water storage tanks (55), and the insertion rods (57) slide inside the box body (51), the third spring (58) is clamped and fixed between the inserted rod (57) and the sealing cover (51), one end of the inserted rod (57) departing from the third spring (58) is in a trapezoidal structure, the inserting groove (59) with the cross-shaped section is arranged in the box body (1), and the button (59 a) with the cross-shaped structure slides in the inserting groove (59); the drawing structure (6) comprises a drawing rod (61), a handle (62), a pull rod (63), a limiting hole (64), a limiting rod (65) and a fourth spring (66), the stretching rod (61) with a T-shaped sliding section at the bottom end of the box body (1), one end of the stretching rod (61) is fixed with the handle (62), the stretching rod (61) is provided with two limit holes (64), the limiting rod (65) with a T-shaped sliding section is arranged in the box body (1), the fourth spring (66) is clamped and fixed between the limiting rod (65) and the box body (1), and the limit rod (65) can be inserted into the limit hole (64), the pull rod (63) is fixed at one end of the limiting rod (65) in a vertical relation, and the pull rod (63) slides with the box body (1); the moving structure (7) comprises a fixed column (71), mounting rods (72), a roller (73), a gear (74), a rack (75), a slider (76) and a fifth spring (77), the fixed column (71) is rotated inside the bottom end of the box body (1), two mounting rods (72) are fixed outside the fixed column (71), the roller (73) is rotated at one end of the mounting rods (72), the roller (73) can be accommodated inside the box body (1), the gear (74) is fixed outside the fixed column (71), the gear (74) rotates with the box body (1), the rack (75) slides inside the box body (1), the rack (75) is engaged with the gear (74), the rack (75) can abut against the stretching rod (61), the slider (76) is fixed at one end of the rack (75) in a perpendicular relationship, and the fifth spring (77) is clamped and fixed between the sliding block (76) and the box body (1).