CN112357314A

CN112357314A - Biological kit for preventing leakage and pollution for experiment and use method thereof

Info

Publication number: CN112357314A
Application number: CN202011221976.3A
Authority: CN
Inventors: 项良成
Original assignee: 项良成
Current assignee: Yingkou Bo'ao medical laboratory Co.,Ltd.
Priority date: 2020-11-05
Filing date: 2020-11-05
Publication date: 2021-02-12
Anticipated expiration: 2040-11-05
Also published as: CN112357314B

Abstract

The invention discloses a biological kit for preventing leakage and pollution for experiments and a using method thereof, the biological kit comprises a box body, wherein a telescopic cavity with an upward opening is arranged in the box body, sliding blocks are arranged on the front side wall and the rear side wall in the telescopic cavity in a sliding manner, a circular cavity with an upward opening is arranged in the sliding block, a driven shaft is rotatably arranged on the bottom wall of the circular cavity, a rotary table is fixedly arranged on the driven shaft, six groups of bearing elements which have the same shape and structure characteristics and can be taken down are arranged on the rotary table, each bearing element comprises a placing block which vertically penetrates through the rotary table, a containing groove with an upward opening is arranged in the placing block, and spring grooves are symmetrically communicated with the left; the reagent is independently stored, when leakage occurs, the reagent is sealed in the reagent box, so that mutual pollution can not occur, the cost can be saved, meanwhile, the reagent can not pollute the environment, and the environment can be protected.

Description

Biological kit for preventing leakage and pollution for experiment and use method thereof

Technical Field

The invention relates to the technical field of biological kits, in particular to a biological kit for preventing leakage and pollution for experiments and a using method thereof.

Background

The biological reagent refers to biological materials or organic compounds related to life science research, and reagents for clinical diagnosis and medical research, and the biological kit is a container for storing the biological reagent, and the traditional biological kit has the following defects:

1. the control temperature mostly uses ice blocks, the temperature is unstable, the fluctuation range is large, and the reagent is not well stored;

2. when the reagent is stored, no independent storage space exists, and when leakage occurs, the reagent can pollute the environment, and meanwhile, the reagents can also mutually pollute each other, and even uncontrollable consequences can occur;

3. the reagents are arranged in sequence, so that reagent leakage caused by mutual collision of test tubes is easy to occur when workers arrange the reagents;

4. there is no function of preventing precipitation by premixing, and precipitation occurs when the reagent is taken out so that the effect of the reagent is lowered.

Disclosure of Invention

The invention aims to provide a biological kit for preventing leakage and pollution in experiments and a using method thereof, which are used for overcoming the defects in the prior art.

The invention relates to a biological kit for preventing leakage and pollution for experiments, which comprises a box body, wherein a telescopic cavity with an upward opening is arranged in the box body, sliding blocks are arranged on the front side wall and the rear side wall in the telescopic cavity in a sliding manner, a circular cavity with an upward opening is arranged in the sliding block, a driven shaft is rotatably arranged on the bottom wall of the circular cavity, a rotary table is fixedly arranged on the driven shaft, six groups of bearing elements with completely same shape and structure characteristics and capable of being taken down are arranged on the rotary table, each bearing element comprises a placing block which vertically penetrates through the rotary table, a containing groove with an upward opening is arranged in the placing block, spring grooves are symmetrically communicated at the left and right positions on the side wall of the containing groove, clamping blocks are slidably arranged in the spring grooves, the clamping blocks are fixedly connected with the spring grooves through springs, the clamping blocks extend inwards into, the symmetrical torsional spring of both sides position is connected with the clamp and grabs around the arc strip, it can take out first belt pulley to be equipped with on the holding tank lateral wall, first belt pulley below is equipped with can from the storage piece that holds inslot and take out, be equipped with the ascending storage chamber of opening in the storage piece, the bilateral position symmetry of holding tank left and right sides is equipped with the drive groove, the drive inslot rotation is installed with the gear shaft, set firmly the gear on the gear shaft, the meshing has the rack above the gear, the rack inwards extends to hold inslot and support above the storage piece, the rack inboard sets firmly first electro-magnet, the sliding block bottom surface left and right sides position symmetry is equipped with the sliding tray that opens downwards, sliding tray slidable mounting has the sliding strip in the sliding tray, the sliding strip extends to in the flexible intracavity and articulates there is telescopic rod group, flexible chamber below intercommunication has the sliding tray, the sliding cavity is internally provided with a fixed column, the fixed column is symmetrically and slidably provided with a thread block at the left side and the right side, the thread block upwards extends to the telescopic cavity and is hinged with the telescopic rod group, the part of the thread block, which is positioned in the telescopic cavity, penetrates through the telescopic cavity and is in threaded connection with a thread shaft rotatably arranged on the right side wall of the telescopic cavity, the thread shaft is in reverse threaded connection with the thread block at the left side, an intermittent element for providing power for the driven shaft is arranged in the sliding block, a linkage element for providing power for the thread shaft is arranged in the sliding block, and a refrigerating element for refrigerating is further arranged.

Optionally, the intermittent type component is including being located the motor chamber of circular chamber below, driven shaft downwardly extending extends to the motor intracavity has set firmly the sheave, six guide ways that the annular was equipped with the opening outwards and runs through from top to bottom on the sheave, be equipped with five openings between the soyasu family guide way outside and the cooperation groove that runs through from top to bottom, the sheave right side be equipped with rotate install in motor shaft on the motor chamber bottom wall, set firmly on the motor shaft can with cooperation groove sliding fit's cooperation piece, cooperation piece right side set firmly can with guide way sliding fit's guide bar, the epaxial top surface of motor be connected with set firmly in the motor of motor chamber roof rotates through intermittent type, makes the box body that the staff was waiting to load before the face always.

Optionally, the linkage element includes a transmission cavity located on the side where the telescopic cavity is located, the thread shaft extends to the right side in the transmission cavity and is fixedly provided with a first bevel gear, a rotating shaft is rotatably installed on the top wall of the transmission cavity, a second bevel gear meshed with the first bevel gear is fixedly arranged on the bottom surface of the rotating shaft, a driving cavity capable of being opened is arranged above the transmission cavity, the rotating shaft extends upwards to the inside of the driving cavity and is fixedly provided with a third bevel gear, a connecting shaft rotationally connected with the box body is fixedly arranged in the driving cavity in a front-back penetrating manner, and a fourth bevel gear meshed with the third bevel gear is fixedly arranged on the part, located in the driving cavity, of the connecting shaft.

Optionally, the refrigeration element includes a refrigeration cavity located on the left side of the telescopic cavity, the refrigeration cavity is communicated with the telescopic cavity through a ventilation net, a motor shaft is rotatably mounted on the left side wall in the refrigeration cavity, fan blades are fixedly arranged on the motor shaft, and the motor shaft extends out of the refrigeration cavity in a left direction and is connected with a motor embedded in the box body.

Optionally, hold the groove rear side and be equipped with the control chamber, gear axial extends to behind the control intracavity and set firmly the second belt pulley, be equipped with between the second belt pulley rotate install in main shaft on the lateral wall behind the control chamber, set firmly on the main shaft with the first belt pulley that the second belt pulley passes through the belt and is connected, set firmly on the wall before the control chamber with the controller that the main shaft is connected.

Optionally, an opening and closing cover capable of being opened is arranged above the box body, a second electromagnet capable of adsorbing the opening and closing cover is arranged in the box body, an open slot with a left opening is formed in the left side of the opening and closing cover, and four ground feet are fixedly arranged on the ground of the box body.

A use method of a biological kit for preventing leakage and pollution in experiments comprises the following specific steps:

the first step, controlling the second electromagnet to not adsorb the opening and closing cover any more, manually opening the opening and closing cover through an opening slot, driving a fourth bevel gear to rotate by a connecting shaft when the opening and closing cover is opened, driving a rotating shaft to transmit by a third bevel gear, driving a first bevel gear to rotate by a second bevel gear, enabling a threaded block to move inwards through thread matching, driving a telescopic rod group to push a sliding strip to move inwards, and starting to prepare for storing a test tube after the sliding block is jacked out of a telescopic cavity;

secondly, starting a motor, driving a matching block to rotate through a motor shaft, enabling a guide rod to enter a guide groove to push a grooved wheel to rotate intermittently, then driving a turntable to rotate intermittently through a driven shaft, enabling a placing block to rotate intermittently, keeping a state to be borne always before a worker, and changing a biological kit when the worker sees that a test tube exists;

thirdly, the test tubes have thickness difference, when the test tubes are placed in the containing groove, the outer walls of the test tubes are in contact with the arc-shaped strips and the clamping claws, the test tubes are in a clamped state through the torsion springs between the clamping claws and the arc-shaped strips, and the test tubes with larger diameters can push the arc-shaped strips to drive the clamping blocks to compress the springs to move towards two sides, so that the clamping is more thorough;

the fourth step, after the saturation, the lid that closes under the lid, connect the axle restaurant, make the screw thread axle reversal, then telescopic link group pulling sliding block downstream, make the second electro-magnet adsorb once more and open and close the lid, the starter motor, the motor shaft drives the refrigeration chamber and rotates, form refrigerated wind-force, make the internal temperature of biological box keep at low temperature state, after the emergence is revealed, the controller starts, the main shaft passes through the belt and drives the second belt pulley rotation, cross the winding on left second belt pulley through the belt, make left second belt pulley rotation opposite direction, then the rack inwards removes, first electro-magnet adsorbs each other, make reagent sealed up the phenomenon that the biological box polluted environment of outflow can not appear.

The invention has the beneficial effects that:

1. the temperature fluctuation range is reduced under the action of the fan, so that the reagent can be better stored without replacing ice cubes;

2. the reagent is stored separately, when leakage occurs, the reagent is sealed in the reagent box, so that mutual pollution is avoided, the cost is saved, and meanwhile, the reagent does not pollute the environment, so that the environment is protected;

3. the round placement enables workers to place easily and reduces errors;

4. through intermittent rotation, the reagent is uniformly fused again when the reagent is about to precipitate, the activity of the reagent is kept, and the experimental efficiency is improved.

Drawings

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.

FIG. 1 is a schematic diagram of a biological reagent kit for preventing contamination in experiments according to the present invention;

FIG. 2 is a schematic view of the structure at A-A in FIG. 1;

FIG. 3 is a schematic view of the structure at B-B in FIG. 1;

FIG. 4 is a schematic view of the structure at C-C in FIG. 1;

fig. 5 is a schematic view of the structure of the driving groove in fig. 1.

Detailed Description

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Referring to fig. 1-5, according to an embodiment of the present invention, a biological reagent kit for preventing leakage and contamination for experiments includes a box body 10, a telescopic cavity 22 with an upward opening is provided in the box body 10, a sliding block 65 is slidably installed in the telescopic cavity 22, a circular cavity 66 with an upward opening is provided in the sliding block 65, a driven shaft 48 is rotatably installed on the bottom wall of the circular cavity 66, a rotary table 36 is fixedly provided on the driven shaft 48, six sets of detachable bearing elements 101 with identical structural characteristics are provided on the rotary table 36, the bearing elements 101 include a placing block 52 vertically penetrating through the rotary table 36, a placing groove 14 with an upward opening is provided in the placing block 52, spring grooves 55 are symmetrically communicated with each other on the side walls of the placing groove 14, a clamping block 53 is slidably installed in the spring groove 55, the clamping block 53 and the spring groove 55 are fixedly connected through a spring 54, the clamping block 53 inwards extends to the containing groove 14 and the arc-shaped strip 15 is fixedly arranged, the symmetrical torsional springs at the front side and the rear side of the arc-shaped strip 15 are connected with clamping claws 51, the side wall of the containing groove 14 is provided with a first belt pulley 67 which can be taken out, the lower side of the first belt pulley 67 is provided with a storage block 20 which can be taken out from the containing groove 14, the storage block 20 is internally provided with a storage cavity 37 with an upward opening, the left side and the right side of the containing groove 14 are symmetrically provided with driving grooves 38, gear shafts 62 are rotatably arranged in the driving grooves 38, gears 61 are fixedly arranged on the gear shafts 62, racks 63 are meshed above the gears 61, the racks 63 inwards extend to the containing groove 14 and abut against the upper side of the storage block 20, the inner sides of the racks 63 are fixedly provided with first electromagnets 64, and sliding grooves 41 with downward openings are symmetrically arranged at the left, a sliding bar 40 is slidably mounted in the sliding groove 41, the sliding bar 40 extends downwards into the telescopic cavity 22 and is hinged with a telescopic rod group 42, a sliding cavity 29 is communicated in the bottom wall of the telescopic cavity 22, a fixing column 28 is fixedly arranged between the left side wall and the right side wall in the sliding cavity 29, the left and right positions of the fixed column 28 are symmetrically and slidably provided with screw blocks 27, the screw blocks 27 extend upwards into the telescopic cavity 22 and are hinged with the telescopic rod group 42, the screw blocks 27 in the telescopic cavity 22 are in threaded connection with a screw shaft 26 rotatably arranged on the right side wall of the telescopic cavity 22, the threaded shaft 26 is connected with the threaded block 27 on the left in a reverse threaded manner, an intermittent element 102 for supplying power to the driven shaft 48 is arranged in the sliding block 65, the box body 10 is internally provided with a linkage element 103 for providing power for the threaded shaft 26, and the box body 10 is also provided with a refrigeration element 104 for refrigeration.

Preferably, the intermittent element 102 includes a motor cavity 43 located below the circular cavity 66, the driven shaft 48 extends downward into the motor cavity 43 and is fixedly provided with a sheave 50, the sheave 50 is annularly provided with six guide grooves 49 which are opened outward and penetrate up and down, five matching grooves 44 which are opened outward and penetrate up and down are arranged between the guide grooves 49, the right side of the sheave 50 is provided with a motor shaft 47 rotatably mounted on the bottom wall of the motor cavity 43, the motor shaft 47 is fixedly provided with a matching block 45 which can be in sliding fit with the matching grooves 44, the side surface of the matching block 45 is fixedly provided with a guide rod 46 which can be in sliding fit with the guide grooves 49, the top surface of the motor shaft 47 is connected with a motor 39 fixedly mounted on the top wall of the motor cavity 43, and by intermittently rotating, a box 10 to be loaded is always in front of a worker, and is in a saturated state when a test tube is seen, the biological kit is then replaced.

Preferably, the linkage element 103 includes a transmission cavity 24 located on the right side of the telescopic cavity 22, the threaded shaft 26 extends rightward into the transmission cavity 24 and is fixedly provided with a first bevel gear 25, the top wall of the transmission cavity 24 is rotatably provided with a rotating shaft 21, the bottom surface of the rotating shaft 21 is fixedly provided with a second bevel gear 23 engaged with the first bevel gear 25, an openable driving cavity 16 is arranged above the transmission cavity 24, the rotating shaft 21 extends upward into the driving cavity 16 and is fixedly provided with a third bevel gear 19, a connecting shaft 18 rotatably connected with the box body 10 penetrates through the driving cavity 16 from front to back, a fourth bevel gear 17 engaged with the third bevel gear 19 is fixedly provided on the part of the connecting shaft 18 located in the driving cavity 16, and the machine is changed into a state to be supported by driving the threaded shaft 26 to rotate.

Preferably, the refrigeration component 104 comprises a refrigeration cavity 35 located on the left side of the telescopic cavity 22, the refrigeration cavity 35 is communicated with the telescopic cavity 22 through a ventilation net 30, a motor shaft 33 is rotatably installed on the left side wall of the refrigeration cavity 35, fan blades 32 are fixedly arranged on the motor shaft 33, the motor shaft 33 extends leftwards out of the refrigeration cavity 35 and is connected with a motor 34 embedded in the box body 10, the temperature in the machine is reduced through wind power, and reagents are stored.

Preferably, the rear side of the containing groove 14 is provided with a control cavity 59, the gear shaft 62 extends backwards into the control cavity 59 and is fixedly provided with a second belt pulley 56, a main shaft 57 rotatably mounted on the rear side wall of the control cavity 59 is arranged between the second belt pulleys 56, a first belt pulley 67 connected with the second belt pulley 56 through a belt 58 is fixedly arranged on the main shaft 57, and a controller 60 connected with the main shaft 57 is fixedly arranged on the front side wall of the control cavity 59.

Preferably, an opening and closing cover 12 capable of being opened is arranged above the box body 10, a second electromagnet 11 capable of adsorbing the opening and closing cover 12 is arranged in the box body 10, an opening groove 13 with a leftward opening is formed in the left side of the opening and closing cover 12, and four ground feet 31 are fixedly arranged on the ground of the box body 10.

firstly, controlling the second electromagnet 11 not to adsorb the opening and closing cover 12 any more, manually opening the opening and closing cover 12 through the opening groove 13, when the opening and closing cover 12 is opened, driving the fourth bevel gear 17 to rotate by the connecting shaft 18, driving the rotating shaft 21 to transmit by the third bevel gear 19, then driving the first bevel gear 25 to rotate by the second bevel gear 23, enabling the thread block 27 to move inwards through thread matching, driving the telescopic rod group 42 to push the sliding strip 40 to move inwards, and starting to prepare for storing test tubes after the sliding block 65 is jacked out of the telescopic cavity 22;

secondly, starting a motor 39, driving a matching block 45 to rotate through a motor shaft 47, enabling a guide rod 46 to enter a guide groove 49 to push a grooved pulley 50 to rotate intermittently, then driving a rotary disc 36 to rotate intermittently through a driven shaft 48, enabling a placing block 52 to rotate intermittently, keeping a state to be borne by a worker all the time, and changing a biological reagent box when the worker sees that a test tube exists;

thirdly, the test tubes have different thicknesses, when the test tubes are placed in the containing groove 14, the outer walls of the test tubes are in contact with the arc-shaped strips 15 and the clamping claws 51, the test tubes are in a clamped state through torsion springs between the clamping claws 51 and the arc-shaped strips 15, the test tubes with larger diameters push the arc-shaped strips 15 to drive the clamping blocks 53 to compress the springs 54 to move towards two sides, and the clamping is more thorough;

and fourthly, after saturation, the cover 12 is opened and closed under the cover, the connecting shaft 18 is reversed, the threaded shaft 26 is reversed, then the telescopic rod group 42 pulls the sliding block 65 to move downwards, the second electromagnet 11 adsorbs the opened and closed cover 12 again, the motor 34 is started, the motor shaft 33 drives the refrigeration cavity 35 to rotate, cooled wind power is formed, the temperature in the biological box is kept in a low-temperature state, after leakage occurs, the controller 60 is started, the main shaft 57 drives the second belt pulley 56 to rotate through the belt 58, the belt 58 is wound on the second belt pulley 56 on the left side in a crossed mode, the rotating directions of the second belt pulley 56 on the left side are opposite, then the rack 63 moves inwards, the first electromagnets 64 adsorb each other, and the reagent is sealed and cannot flow out to pollute the environment of the biological box.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. The utility model provides an it is experimental with preventing biological reagent box of leakage pollution, includes the box body, be equipped with the ascending flexible chamber of opening in the box body, lateral wall slidable mounting is equipped with the sliding block around flexible intracavity, be equipped with the ascending circular chamber of opening in the sliding block, it installs the driven shaft to rotate on the circular chamber diapire, the carousel has set firmly on the driven shaft, be equipped with six groups shape structural feature identical bearing element that can take off on the carousel, its characterized in that: the bearing element comprises a placing block which vertically penetrates through the rotary disc, a placing groove with an upward opening is arranged in the placing block, the left and right positions on the side wall of the placing groove are symmetrically communicated with a spring groove, a clamping block is slidably mounted in the spring groove, the clamping block and the spring groove are fixedly connected through a spring, the clamping block inwards extends into the placing groove and is fixedly provided with an arc strip, the front and rear side positions of the arc strip are symmetrically connected with a clamping claw through torsion springs, a first belt pulley capable of being taken out is arranged on the side wall of the placing groove, a storage block capable of being taken out from the placing groove is arranged below the first belt pulley, a storage cavity with an upward opening is arranged in the storage block, the left and right side positions of the placing groove are symmetrically provided with driving grooves, a gear shaft is rotatably mounted in the driving grooves, a gear is fixedly arranged on the gear shaft, and, the rack extends inwards to the containing groove and is abutted against the upper side of the storage block, a first electromagnet is fixedly arranged on the inner side of the rack, sliding grooves with downward openings are symmetrically arranged at the left and right positions of the bottom surface of the sliding block, sliding strips are slidably arranged in the sliding grooves, the sliding strips extend downwards to the inside of the telescopic cavity and are hinged with telescopic rod sets, a sliding cavity is communicated with the lower part of the telescopic cavity, fixed columns are fixedly arranged on the left and right side walls in the sliding cavity, threaded blocks are symmetrically and slidably arranged at the left and right positions on the fixed columns, the threaded blocks extend upwards to the inside of the telescopic cavity and are hinged with the telescopic rod sets, the part of the threaded blocks, which is positioned in the telescopic cavity, penetrates through and is in threaded connection with threaded shafts rotatably arranged on the right side wall of the telescopic cavity, the threaded shafts are in reverse threaded connection with the threaded blocks on the left side, the interior is equipped with for the linkage element of screw shaft power, still be equipped with the refrigeration component that is used for the refrigeration.

2. A contamination prevention biological kit for experiments according to claim 1, wherein: intermittent type component is including being located the motor chamber of circular chamber below, driven shaft downwardly extending has set firmly the sheave in the motor chamber, six guide ways that the annular was equipped with the opening and outwards just runs through from top to bottom on the sheave, be equipped with five openings between the soyasu family guide way and outwards just the cooperation groove that runs through from top to bottom, the sheave right side be equipped with rotate install in motor shaft on the motor chamber bottom wall, motor shaft on set firmly can with cooperation groove sliding fit's cooperation piece, cooperation piece right side set firmly can with guide way sliding fit's guide bar, motor shaft is gone up the top surface be connected with set firmly in the motor of motor chamber roof rotates through intermittent type, makes the box body that the staff was waiting to load before the face always.

3. A contamination prevention biological kit for experiments according to claim 1, wherein: the linkage element comprises a transmission cavity located on the side where the telescopic cavity is located, the thread shaft extends to the right side in the transmission cavity and is fixedly provided with a first bevel gear, a rotating shaft is rotatably installed on the top wall of the transmission cavity, a second bevel gear meshed with the first bevel gear is fixedly arranged on the bottom surface of the rotating shaft, a driving cavity capable of being opened is arranged above the transmission cavity, the rotating shaft extends upwards to the inside of the driving cavity and is fixedly provided with a third bevel gear, a connecting shaft rotationally connected with the box body is fixedly arranged in the driving cavity in a front-back penetrating mode, and a fourth bevel gear meshed with the third bevel gear is fixedly arranged on the part, located in the driving cavity, of the connecting shaft.

4. A contamination prevention biological kit for experiments according to claim 1, wherein: the refrigeration component comprises a refrigeration cavity which is located on the left side of the telescopic cavity, the refrigeration cavity is communicated with the telescopic cavity through a ventilation net, a motor shaft is rotatably installed on the left side wall in the refrigeration cavity, fan blades are fixedly arranged on the motor shaft, and the motor shaft extends out of the refrigeration cavity in a left direction and is connected with a motor embedded in the box body.

5. A contamination prevention biological kit for experiments according to claim 1, wherein: hold the groove rear side and be equipped with the control chamber, the gear axial extends to behind the control intracavity and set firmly the second belt pulley, be equipped with between the second belt pulley rotate install in main shaft on the lateral wall behind the control chamber, set firmly on the main shaft with the first belt pulley that the second belt pulley passes through the belt and is connected, set firmly on the wall before the control chamber with the controller that the main shaft is connected.

6. A contamination prevention biological kit for experiments according to claim 1, wherein: the box body top is equipped with the lid that opens and shuts that can open, be equipped with in the box body and adsorb the second electro-magnet of lid that opens and shuts, it is equipped with the open slot that the opening is left to open and shut the lid left side, box body ground has set firmly four lower margins.

7. The use method of the biological kit for preventing the leakage and the pollution in the experiment is characterized by comprising the following specific steps of: