CN206476981U - A kind of kit detected for B cell leukemia minimal residual disease - Google Patents
A kind of kit detected for B cell leukemia minimal residual disease Download PDFInfo
- Publication number
- CN206476981U CN206476981U CN201720025086.2U CN201720025086U CN206476981U CN 206476981 U CN206476981 U CN 206476981U CN 201720025086 U CN201720025086 U CN 201720025086U CN 206476981 U CN206476981 U CN 206476981U
- Authority
- CN
- China
- Prior art keywords
- hinged
- box
- movable block
- cell leukemia
- residual disease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of kit detected for B cell leukemia minimal residual disease, including box body, lid is hinged with the box body, placed cavity is provided with the box body, refrigerating box is provided with the placed cavity, in the placed cavity fixed plate is equipped with relative side wall, the fixed plate is provided with the first connector, cylinder is hinged with first connector, the side of the lid is provided with two the second connectors being parallel to each other, the piston-rod end of the cylinder is hinged on the second connector, movable block is provided with the refrigerating box, cavity is provided with the movable block, drive device is provided with the cavity, the output shaft of the drive device is provided with first gear.The function of being implemented in combination with automatically opening up kit that the utility model passes through cylinder, drive device and folded sheet; the setting for placing plate and damping spring etc. simultaneously not only facilitates operation; the effect of damping is also acted as, the reagent bottle of the inside is protected, it is suitable to promote.
Description
Technical field
The utility model is related to biological technical field, more particularly to a kind of B cell leukemia minimal residual disease that is used for is detected
Kit.
Background technology
Bone-marrow-derived lymphocyte also can abbreviation B cell.From the multipotential stem cell of marrow.Birds are that life is developed in the bursa of farbricius
Into, therefore also known as bursa-dependent lymphocyte/marrow dependence lymphocyte abbreviation B cell, it is by the candidate stem cell in marrow point
Change development.Compared with T lymphocytes, its volume is bigger.After this lymphocyte receptor antigenic stimulus, meeting Proliferation, Differentiation goes out
A large amount of thick liquid cells.Thick liquid cell can be synthesized with secretory antibody and circulated in blood.B cell lymphoma is a kind of most common lymph
Chronic myeloid leukemia, the research about this disease is continued to bring out.In mammality developed in the tissue such as marrow of class capsule structure
's.Also known as bone marrowdependent lymphocyte.The stem cell come from marrow or pre B cell, after the bursa of farbricius or bursa-equivalent organ is moved into, by
Step is divided into the B cell of immunological competence.Ripe B cell is moved out through peripheral blood, into spleen, lymph node, is distributed mainly on
In lymph nodule under splenic corpuscle, splenic cords and lymph nodule, lymphatic cord and gastrointestinal mucosal, after antigenic stimulus, differentiation and proliferation
For thick liquid cell, synthetic antibody plays the function of humoral immunity.The most of vaccines used at present are exactly by stimulating this kind of B to drench
Bar cell produces antibody.Quantity of the B cell in marrow and aggregated lymphatic follicles is more compared with T cell, in blood and lymph node
Quantity is fewer than T cell, then less in ductus thoracicus, only a small number of to participate in recycling.There are many different marks on the cell membrane of B cell
Will, mainly surface antigen and surface receptor.These surface markers are all incorporated in the huge protein molecular on cell membrane.B1 cells
For T cell independence cell.B2 is T cell dependent cell.The time that B cell is survived in vivo is shorter, and only a couple of days is to number
Week, but its memory cell in vivo can long-term existence, at present to B cell leukemia residual detect when typically use reagent
Box is detected that still, existing reagent cartridge configuration is simple, and function is single, only with bearing function, it is impossible to reagent is carried out cold
Tibetan is handled, very inconvenient to use with less the function of automatic opening, therefore, being used for B cell leukemia we have proposed one kind
The kit of minimal residual disease detection solves the above problems.
Utility model content
The purpose of this utility model is that, in order to solve shortcoming present in prior art, and the one kind proposed is used for B cell
The kit of Minimal Residual Disease of Leukemia detection.
To achieve these goals, the utility model employs following technical scheme：
Box is hinged with a kind of kit detected for B cell leukemia minimal residual disease, including box body, the box body
It is provided with lid, the box body in placed cavity, the placed cavity and is provided with refrigerating box, is equipped with the placed cavity on relative side wall
Fixed plate, the fixed plate is provided with the first connector, first connector and is hinged with cylinder, and the side of the lid is set
There are two the second connectors being parallel to each other, the piston-rod end of the cylinder is hinged on the second connector, the refrigerating box
Interior be provided with movable block, the movable block is provided with cavity, and drive device, the output shaft of the drive device are provided with the cavity
It is provided with to run through on first gear, the movable block and is provided with rotating bar, the two ends of the rotating bar on the outside of movable block is equipped with
Roller, the rotating bar in cavity is provided with second gear, first gear and the second gear intermeshing, the refrigeration
The chute corresponding with roller is equipped with box on relative side wall, and roller is located in chute, the side of the movable block connects
Folded sheet is connected to, the one end of the folded sheet away from movable block is fixed on the side wall in refrigerating box, in the refrigerating box
Provided with refrigerating plant.
Preferably, the side of the box body is hinged with loading plate, what the side wall of the box body was parallel to each other provided with two
Bobbin winoler, the bobbin winoler is provided with drawstring, and the one end of the drawstring away from bobbin winoler is fixed on loading plate, the loading plate
On be equidistantly provided with multiple first standing grooves, the side of first standing groove is provided with two the second standing grooves, and described second puts
Groove is put more than the first standing groove.
Preferably, multiple upper cushion blocks, the side of the upper cushion block are equidistantly provided with side wall relative in the placed cavity
Damping spring is fixed with, the one end of the damping spring away from upper cushion block is fixed with lower cushion block, and one end of the lower cushion block is fixed
On the side wall of refrigerating box.
Preferably, the lid, which is provided with stopper slot, the stopper slot, is hinged with handle, and the handle is provided with anti-skidding
Line.
Preferably, it is equipped with heat-insulation layer on the side wall in the refrigerating box.
In the utility model, when being operated, cylinder can be opened lid by the flexible of piston rod, while bobbin winoler is logical
Cross drawstring to put down loading plate, drive device drives first gear to rotate by the rotation of output shaft, the rotational band of first gear
Dynamic second gear is rotated, so as to drive roller to be slided in chute, and then folded sheet is folded, operating personnel take out of refrigerating box
Go out reagent bottle, be positioned in corresponding standing groove and detected according to size, the utility model by cylinder, drive device and
The function of being implemented in combination with automatically opening up kit of folded sheet, while the setting for placing plate and damping spring etc. is not only square
Operation, also act as the effect of damping, protect the reagent bottle of the inside, it is suitable to promote.
Brief description of the drawings
Fig. 1 be the utility model proposes it is a kind of for B cell leukemia minimal residual disease detect kit structure
Schematic diagram；
Fig. 2 be the utility model proposes it is a kind of for B cell leukemia minimal residual disease detect kit inside
Structural representation；
Fig. 3 be the utility model proposes it is a kind of for B cell leukemia minimal residual disease detect kit damping
Apparatus structure schematic diagram.
In figure：1 lid, 2 loading plates, 3 handles, 4 stopper slots, 5 box bodys, 6 cylinders, 7 fixed plates, 8 bobbin winolers, 9 drawstrings,
10 refrigerating box, 11 damping springs, 12 movable blocks, 13 folded sheets.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of the utility model, rather than whole
Embodiment.
Reference picture 1-3, a kind of kit detected for B cell leukemia minimal residual disease, is below the utility model
The specifically used step of kit:
1st, One step RT-PCR step；
2nd, magnetic beads for purifying step
1) amount for taking out magnetic bead vibration mixing 5min 45 μ l/ samples of taking-up is put in room temperature 10min；
2) RT-PCR product respectively takes out 20 μ l, remaining 5 μ l justincase, then each sample adds 30 μ lH2O, most
Magnetic bead is added according to 45 μ l/ afterwards, is inhaled with rifle and blows about 10 mixings, be put in room temperature 2min；
3) uncap and be put in 1min or so on magnetic frame, liquid, which becomes clarification, can wash out liquid, add 125 μ l85% second
Wash out and discard after alcohol, 1min, magnetic bead is put in room temperature 8min (5-10min) and dried.
3rd, DNA concentration is determined with Nanodrop.If concentration is 20ng/ μ l, 5 times are diluted；If close to 40ng/ μ l,
10 times of dilution.
4th, end is repaired：Following component (50 μ l) is mixed in 1.5ml centrifuge tubes
1) 34 μ l are purifiedPatient's sample genomeDNA (or moisturizing to 34 μ l)
2) buffer solution is repaired in 5 μ l10 × end
3) 5 μ l2.5mMdNTP mixtures
4)5μl10mMATP
5) 1 μ l ends repair enzyme
Room temperature is placed 45 minutes.Purified with QIAquickPCR purification columns, eluted with 34 μ l buffer solutions.
5th, 3 ' ends add A：Following component (50 μ l) is mixed in 1.5ml centrifuge tubes：
1) DNA after the end of 34 μ l steps 4 is repaired
2) 5 μ lKlenow buffer solutions=NEBbuffer2
3)10μl1mMdATP
4) 1 μ lKlenow fragments (5 prime excision enzyme activities of 3 ' à 5 ')
(1mMdATP with 100mMdATP mother liquors dilute, often the μ l of pipe 25 dispense, can only freeze thawing once)
Purified with MinElutePCR purification columns, the elution of 12 μ l buffer solutions.
6th, joint is connected：Following component (30ul) is mixed in 1.5ml centrifuge tubes
1) DNA of 11 μ l steps 5
2) 15 μ lDNA connection buffer solutions
3)1μl1:The linker oligonucleotides of 20 dilutions
4) 3 μ lDNA ligases
If doing multiple repetitions simultaneously, it is ensured that each parallel reaction adds different joints, marks clear.
7th, gel screening removes unnecessary joint：
The 6 μ l sample-loading buffers for diluting 10 times are added in the reaction solution of the steps of 30 μ l the 6th, loading to two E-gel glue
Kong Zhong.The 50bpDNAladder loadings for taking 20 μ l to dilute 10 times in addition.Electrophoresis 20 minutes.
DNA of the size between 150bp to 450bp is cut, is purified with QIAquick gel purification kits,
30 μ l elution buffers are eluted.
8th, Illumina primer PCRs are used：
IlluminaPCR primers 1.1 and 2.1 are carried out 1 with Gibco water：1 is diluted in PCR pipe and mixes following component
(60ul)：
1) DNA of 30 μ l steps 4
2) 28 μ lPhusionPCR premixed liquids
3) primer 1.1 of 1 μ l dilutions
4) the primer 2 .1 of 1 μ l dilutions
PCR cycle:
9th, molecular weight screening is carried out on 2% Ago-Gel：
The 1 μ l sample-loading buffers diluted are added in the PCR reaction solutions of previous step, loading is into three E-gel glue holes.
50bp the and 100bpDNAladder loadings for taking 20 μ l to dilute 10 times respectively in addition.Electrophoresis 30 minutes.Size is cut in 400bp
The DNA of left and right.Purify DNA with QIAquick gel purification kits, eluted with 30 μ l elution buffers.
10th, DNA concentration is determined.Gained DNA concentration is determined with NanoDrop.
In the utility model, when being operated, cylinder 6 can be opened lid 1 by the flexible of piston rod, while coiling
Device 8 is put down loading plate 2 by drawstring 9, and drive device drives first gear to rotate by the rotation of output shaft, first gear
Rotate and drive second gear to rotate, so as to drive roller to be slided in chute, and then folded sheet 13 is folded, operating personnel are from cold
Hide and reagent bottle is taken out in box 10, be positioned in corresponding standing groove and detected according to size.
It is described above, only the utility model preferably embodiment, but protection domain of the present utility model is not
This is confined to, any one skilled in the art is in the technical scope that the utility model is disclosed, according to this practicality
New technical scheme and its utility model design are subject to equivalent substitution or change, should all cover in protection model of the present utility model
Within enclosing.
Claims (5)
1. a kind of kit detected for B cell leukemia minimal residual disease, including box body (5), it is characterised in that the box
It is hinged with body (5) in lid (1), the box body (5) and is provided with placed cavity, refrigerating box (10) is provided with the placed cavity, it is described
Fixed plate (7) is equipped with placed cavity on relative side wall, the fixed plate (7) is provided with the first connector, and described first connects
Cylinder (6) is hinged with fitting, the side of the lid (1) is provided with two the second connectors being parallel to each other, the cylinder
Piston-rod end is hinged on the second connector, is provided with the refrigerating box (10) in movable block (12), the movable block (12)
Provided with cavity, drive device is provided with the cavity, the output shaft of the drive device is provided with first gear, the movable block
(12) run through on and be provided with rotating bar, the two ends of the rotating bar on the outside of movable block (12) are equipped with roller, in cavity
Rotating bar is provided with side relative in second gear, first gear and the second gear intermeshing, the refrigerating box (10)
The chute corresponding with roller is equipped with wall, and roller is located in chute, the side of the movable block (12) is connected with folding
Plate (13), the one end of the folded sheet (13) away from movable block (12) is fixed on the side wall in refrigerating box (10), described
Refrigerating plant is provided with refrigerating box (10).
2. a kind of kit detected for B cell leukemia minimal residual disease according to claim 1, its feature exists
In the side of the box body (5) is hinged with loading plate (2), and the side wall of the box body (5) is provided with two coilings being parallel to each other
Device (8), the bobbin winoler (8) is provided with drawstring (9), and the one end of the drawstring (9) away from bobbin winoler (8) is fixed on loading plate
(2) multiple first standing grooves are equidistantly provided with, on the loading plate (2), the side of first standing groove is provided with two the
Two standing grooves, second standing groove is more than the first standing groove.
3. a kind of kit detected for B cell leukemia minimal residual disease according to claim 1, its feature exists
In equidistantly provided with multiple upper cushion blocks on relative side wall in the placed cavity, the side of the upper cushion block is fixed with damping bullet
Spring (11), the one end of the damping spring (11) away from upper cushion block is fixed with lower cushion block, and one end of the lower cushion block is fixed on cold
On the side wall for hiding box (10).
4. a kind of kit detected for B cell leukemia minimal residual disease according to claim 1, its feature exists
In the lid (1) is provided with stopper slot (4), the stopper slot (4) and is hinged with handle (3), and the handle (3) is provided with
Anti-slip veins.
5. a kind of kit detected for B cell leukemia minimal residual disease according to claim 1, its feature exists
In being equipped with heat-insulation layer on the side wall in the refrigerating box (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720025086.2U CN206476981U (en) | 2017-01-10 | 2017-01-10 | A kind of kit detected for B cell leukemia minimal residual disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720025086.2U CN206476981U (en) | 2017-01-10 | 2017-01-10 | A kind of kit detected for B cell leukemia minimal residual disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206476981U true CN206476981U (en) | 2017-09-08 |
Family
ID=59750684
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720025086.2U Expired - Fee Related CN206476981U (en) | 2017-01-10 | 2017-01-10 | A kind of kit detected for B cell leukemia minimal residual disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206476981U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115465572A (en) * | 2022-09-02 | 2022-12-13 | 湖南省产商品质量检验研究院 | Rapid detection kit for pesticide residues and use method thereof |
-
2017
- 2017-01-10 CN CN201720025086.2U patent/CN206476981U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115465572A (en) * | 2022-09-02 | 2022-12-13 | 湖南省产商品质量检验研究院 | Rapid detection kit for pesticide residues and use method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206300444U (en) | A kind of endocrine censorship test tube sterilizing and drying device | |
| US20110114877A1 (en) | Plasmid DNA Isolation | |
| CN206476981U (en) | A kind of kit detected for B cell leukemia minimal residual disease | |
| CN107249746A (en) | System and method for collecting sample of nucleic acid | |
| CN206474208U (en) | A kind of practical clinical examination rack for test tube | |
| CN109929813A (en) | Salmonella bacteriophage nanometer magnetic bead conjugate and its concentration and separation kit | |
| CN107512724A (en) | One kind is used to remove CO in admixture of gas2Silica zeolite | |
| CN207760321U (en) | The reagent disc of pre-treatment is automated for detection of nucleic acids | |
| CN208869582U (en) | The micro-fluidic chip of bacterium total nucleic acid in a kind of extraction human whole blood | |
| CN206529458U (en) | A kind of kit detected for T cell Minimal Residual Disease of Leukemia | |
| CN107603975A (en) | A kind of lily method for extracting total RNA | |
| CN110699243B (en) | Multifunctional nucleic acid detection device | |
| CN103898191B (en) | Detection Methods of Salmonella and detection substratum | |
| CN209514935U (en) | A kind of bioconversion medium sample displaying device | |
| CN207641522U (en) | A kind of test device of medical test | |
| CN208658280U (en) | A kind of chemical reagent storing compartment | |
| CN207970897U (en) | A kind of centrifuge tube extraction plate | |
| Wang et al. | Use of automated riboprinter and pulsed-field gel electrophoresis for epidemiological studies of invasive Haemophilus influenzae in Taiwan | |
| CN209401184U (en) | A kind of medical teaching parasite specimen displaying device | |
| CN206375908U (en) | A kind of low temperature hybridization instrument | |
| CN211984573U (en) | Categorised specimen cabinet | |
| CN108034581A (en) | A kind of device for being used to detect detection of Salmonella | |
| CN215664635U (en) | ARGs absolute quantitative kit suitable for 96-channel qPCR instrument | |
| CN220967410U (en) | Nursing medicine chest | |
| CN216071059U (en) | Rotavirus detection kit in intestinal tract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170908 Termination date: 20210110 |