CN115465572A - Rapid detection kit for pesticide residues and use method thereof - Google Patents
Rapid detection kit for pesticide residues and use method thereof Download PDFInfo
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- CN115465572A CN115465572A CN202211074253.4A CN202211074253A CN115465572A CN 115465572 A CN115465572 A CN 115465572A CN 202211074253 A CN202211074253 A CN 202211074253A CN 115465572 A CN115465572 A CN 115465572A
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- test tube
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- box
- pesticide residue
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- 239000000447 pesticide residue Substances 0.000 title claims abstract description 38
- 238000001514 detection method Methods 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000003860 storage Methods 0.000 claims abstract description 90
- 238000012360 testing method Methods 0.000 claims abstract description 71
- 238000010438 heat treatment Methods 0.000 claims abstract description 29
- 239000003814 drug Substances 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 13
- 238000005070 sampling Methods 0.000 claims description 8
- 235000012055 fruits and vegetables Nutrition 0.000 claims description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 102100032404 Cholinesterase Human genes 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000000151 deposition Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 101710083761 Cholinesterase Proteins 0.000 claims 1
- 235000013311 vegetables Nutrition 0.000 abstract description 9
- 239000000575 pesticide Substances 0.000 abstract description 5
- 108010053652 Butyrylcholinesterase Proteins 0.000 abstract description 4
- 235000013399 edible fruits Nutrition 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000005764 inhibitory process Effects 0.000 abstract description 2
- 102000021944 Butyrylcholinesterase Human genes 0.000 abstract 1
- 239000000047 product Substances 0.000 description 6
- 235000013305 food Nutrition 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 102100033639 Acetylcholinesterase Human genes 0.000 description 1
- 108010022752 Acetylcholinesterase Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 1
- 229960004373 acetylcholine Drugs 0.000 description 1
- 229940022698 acetylcholinesterase Drugs 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000152 carbamate pesticide Substances 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 231100000739 chronic poisoning Toxicity 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007830 nerve conduction Effects 0.000 description 1
- 239000003987 organophosphate pesticide Substances 0.000 description 1
- 210000001428 peripheral nervous system Anatomy 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/36—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents adapted to be used for non-packaging purposes after removal of contents
- B65D81/368—Foldable carton-boxes simulating, or transformable into, a special configuration, e.g. a car, a house, an animal
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
- B65D25/108—Devices, e.g. plates, presenting apertures through which the articles project
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/28—Handles
- B65D25/2835—Swingable handles
- B65D25/2852—Swingable handles provided on a local area near to or at the upper edge or rim
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D77/00—Packages formed by enclosing articles or materials in preformed containers, e.g. boxes, cartons, sacks or bags
- B65D77/04—Articles or materials enclosed in two or more containers disposed one within another
- B65D77/0446—Articles or materials enclosed in two or more containers disposed one within another the inner and outer containers being rigid or semi-rigid and the outer container being of polygonal cross-section not formed by folding or erecting one or more blanks
- B65D77/0453—Articles or materials enclosed in two or more containers disposed one within another the inner and outer containers being rigid or semi-rigid and the outer container being of polygonal cross-section not formed by folding or erecting one or more blanks the inner container having a polygonal cross-section
- B65D77/046—Articles or materials enclosed in two or more containers disposed one within another the inner and outer containers being rigid or semi-rigid and the outer container being of polygonal cross-section not formed by folding or erecting one or more blanks the inner container having a polygonal cross-section and being a tray or like shallow container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/34—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents for packaging foodstuffs or other articles intended to be cooked or heated within the package
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- Food Science & Technology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a rapid detection kit for pesticide residues and a using method thereof, and relates to the technical field of pesticide residue detection. The invention discloses a rapid pesticide residue detection kit which comprises a storage box, wherein a storage mechanism for storing a test tube and a detection medicament is arranged at the top of the storage box, a first box cover is hinged to the top of the storage box, and a sample storage mechanism is arranged on one side wall of the storage box. According to the invention, the operation of the sample is further facilitated during pesticide residue detection through the operation table component, so that the use of detection personnel is further facilitated, the solution during detection is further conveniently heated through the heating mechanism, the pain-sensitive object placing mechanism is further convenient for storing appliances during detection, the kit is further convenient to carry through the handle, the pesticide residue condition contained in the sample can be known through butyrylcholinesterase, so that the activity inhibition degree of pesticide endoenzyme can be conveniently measured, and further, the detection of various vegetables and fruits is facilitated.
Description
Technical Field
The invention relates to the technical field of pesticide residue detection, in particular to a pesticide residue rapid detection kit and a using method thereof.
Background
With the development of society and the improvement of living standard of people, people are more and more aware of the safety and legal awareness of agricultural products, vegetables are more and more concerned as indispensable consumer goods for three meals a day in the life of the people, due to various factors, the safety of the quality of the vegetables cannot be completely guaranteed, the vegetables with overproof edible residual pesticides can cause chronic poisoning and directly threaten the health of people, in order to control the production of toxic and harmful vegetables, vegetable pesticide residue detection work must be carried out, and pesticide residue refers to the phenomenon that after pesticides are applied in agricultural production, a part of pesticides directly or indirectly remain in grains, vegetables, fruits, livestock products, soil and water.
When food safety supervision is carried out on food retail outlets such as agricultural and sideline product wholesale markets, supermarkets and the like, on-site sampling and rapid detection are required to be configured, the on-site rapid detection is used as a first step for finding food safety problems, and the existing spectrophotometer for detecting agricultural and sideline products needs to be matched with a complex detection reagent and is complex to use.
The organophosphorus or carbamate pesticide can inhibit the activity of acetylcholinesterase in central and peripheral nervous system of insect, cause accumulation of nerve conduction medium acetylcholine, affect normal conduction and kill insect poisoning.
Therefore, a rapid detection kit for pesticide residues and a using method thereof are provided.
Disclosure of Invention
The invention aims to: the invention provides a rapid pesticide residue detection kit and a use method thereof, aiming at solving the problem that the existing detection of pesticide residues in agricultural and sideline products is complex.
The invention specifically adopts the following technical scheme for realizing the purpose:
the utility model provides a quick detect reagent box of pesticide residue, includes the case, the top of case is provided with the receiving mechanism who is used for depositing the test tube and detects the medicament, articulated first lid of installing in the top of case, a lateral wall of case is provided with sample storage mechanism, the preceding lateral wall of case is provided with the operation panel subassembly that is used for operation when detecting pesticide residue, who is having the side box another lateral wall of case, the preceding lateral wall of side box is provided with and is used for pesticide residue detection utensil to deposit puts thing mechanism, the top of side box is provided with heating mechanism, the articulated second lid of installing in the top of side box, a lateral wall of case articulates with another lateral wall of side box and installs the handle.
Further, receiving mechanism includes test tube unable adjustment base, the inboard bottom fixed mounting of case has test tube unable adjustment base, fixed recess has been seted up at test tube unable adjustment base's top, the inner wall fixed mounting of case has first test-tube rack, first test tube fixing hole has been seted up at the top of first test-tube rack, first test tube fixing hole has the multiunit with fixed recess's quantity, and the first test tube fixing hole of multiunit is corresponding the setting with fixed recess.
Further, the sample storage mechanism comprises a storage cavity, the storage cavity is formed in the storage box, a storage drawer is movably inserted in the storage cavity, a first sealing plate is arranged on one side wall of the storage drawer, and when the storage drawer and the first sealing plate are closed, the first sealing plate is flush with one side wall of the storage box.
Further, the operation panel subassembly includes the standing groove, the standing groove has been seted up to the preceding lateral wall of case, the articulated operation table of installing of the left and right sides wall of standing groove, the handle groove has been seted up to the bottom of operation table, the left and right sides wall fixed mounting of standing groove has the connection rope, the free end of connecting the rope sets up with the top grafting fixed mounting of operation table, and connects the rope and adopt flexible material to make.
Further, the object placing mechanism comprises an object placing cavity, the front side wall of the side box is provided with the object placing cavity, the object placing box is movably arranged inside the object placing cavity, and a second sealing plate is fixedly arranged on the front side wall of the object placing box.
Further, heating mechanism is including heating the cavity, heating the cavity has been seted up at the top of side box, heating the surface laminating of cavity has the hot plate, the inner wall fixed mounting of hot plate has the second test-tube rack, second test tube fixing hole has been seted up at the top of second test-tube rack.
Further, the application method of the pesticide residue rapid detection kit comprises the following steps:
s1: sampling; when sampling, samples from different plant leaves (at least one to eight leaves) are taken by a stainless steel tube sampler, fruits and vegetables are sampled from the skin to the pulp at a position of one to one point and five centimeters, and the sampled fruit and vegetable samples are stored in a sample storage mechanism;
s2: processing a sample; taking out the sample stored in the sample storage mechanism, unfolding the operating platform assembly, placing the sample on the operating platform assembly, chopping the sample through a prop, taking out a test tube from the storage mechanism, and adding the chopped sample into the test tube by four grams;
s3: extracting a sample extracting solution; adding twenty milliliters of buffer solution into a test tube in which a sample is stored, manually shaking for two minutes, pouring the extracting solution into a small test tube for three milliliters, and standing the small test tube for three to five minutes;
s4: detecting; fifty microliters of butyrylcholinesterase and fifty microliters of color-developing agent are added into a small test tube in which the extracting solution is stored, the small test tube is placed into a heating mechanism, placed for five minutes at a constant temperature of thirty-eight degrees, then fifty microliters of substrate is added, the small test tube is poured into a cuvette, and the small test tube is vibrated and then measured by using a pesticide residue quick-measuring instrument.
Furthermore, in step S4, the small tubes should be rapidly mixed when the substrate is added.
The invention has the following beneficial effects:
1. according to the invention, the storage of the test tube and the reagent is realized through the storage mechanism, the storage of samples sampled from fruits, vegetables and the like is realized through the sample storage mechanism, the operation of the samples is further facilitated when the pesticide residue is detected through the operation table component, the use of a detector is further facilitated, the solution is further facilitated when the detection is performed through the heating mechanism, the storage mechanism with pain feeling is further facilitated for storing instruments when the detection is performed, the kit is further facilitated to be carried through a handle, the pesticide residue condition contained in the samples can be known through the butyrylcholinesterase, the degree of inhibition of the activity of the enzyme in the pesticide is further facilitated to be measured, and the detection of various fruits and vegetables is further facilitated.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic side view of the present invention;
FIG. 3 is a schematic structural view in elevation and section of the present invention;
FIG. 4 is an enlarged view of the portion A of the present invention;
reference numerals are as follows: 1. a storage box; 2. a storage mechanism; 201. a test tube fixing base; 202. fixing the groove; 203. a first test tube rack; 204. a first test tube fixing through hole; 3. a first cover; 4. a sample storage mechanism; 401. a storage chamber; 402. storing the drawer; 403. a first sealing plate; 5. an operating floor assembly; 501. a placement groove; 502. operating the table plate; 503. a handle slot; 504. connecting ropes; 6. a side box; 7. a placement mechanism; 701. a storage cavity; 702. a storage box; 703. a second sealing plate; 8. a heating mechanism; 801. heating the cavity; 802. heating plates; 803. a second test tube rack; 804. a second test tube fixing through hole; 9. a second cover; 10. a handle.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined or explained in subsequent figures. Furthermore, the terms "first," "second," and the like are used solely to distinguish one from another, and are not to be construed as indicating or implying relative importance.
In the description of the embodiments of the present invention, it should be noted that the terms "inside", "outside", "upper", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships conventionally arranged when products of the present invention are used, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements indicated must have specific orientations, be constructed in specific orientations, and operated, and thus, cannot be construed as limiting the present invention.
As shown in fig. 1 to 4, a pesticide residue rapid detection kit and a using method thereof, including a storage box 1, a storage mechanism 2 for storing test tubes and detecting chemicals is arranged at the top of the storage box 1, a first box cover 3 is hinged at the top of the storage box 1, a sample storage mechanism 4 is arranged on one side wall of the storage box 1, an operation table component 5 for operation in pesticide residue detection is arranged on the front side wall of the storage box 1, a side box 6 is arranged on the other side wall of the storage box 1, a storage mechanism 7 for storing pesticide residue detectors is arranged on the front side wall of the side box 6, a heating mechanism 8 is arranged at the top of the side box 6, a second box cover 9 is hinged at the top of the side box 6, and a handle 10 is hinged at one side wall of the storage box 1 and the other side wall of the side box 6; specifically, accomodate test tube and reagent through receiving mechanism 2 realization, deposit mechanism 4 through the sample and then realize depositing the sample of sample such as fruit vegetables, through operation panel subassembly 5 and then be convenient for pesticide residue to detect time measuring and operate the sample, and then be convenient for the measurement personnel to use, through heating mechanism 8 and then be convenient for heat the solution that detects time measuring, deposit the utensil when detecting through putting thing mechanism 7 and then being convenient for, through handle 10 and then be convenient for carry this kit.
As shown in fig. 3, the accommodating mechanism 2 includes a test tube fixing base 201, the bottom of the inner side of the storage box 1 is fixedly provided with the test tube fixing base 201, the top of the test tube fixing base 201 is provided with a fixing groove 202, the inner wall of the storage box 1 is fixedly provided with a first test tube rack 203, the top of the first test tube rack 203 is provided with a first test tube fixing through hole 204, the number of the first test tube fixing through holes 204 and the fixing groove 202 is multiple groups, and the multiple groups of the first test tube fixing through holes 204 and the fixing groove 202 are correspondingly arranged; specifically, correspond and then be convenient for to take the test tube and deposit the medicine bottle of reagent through fixed recess 202 and first test tube fixing hole 204 and deposit.
As shown in fig. 3, the sample storage mechanism 4 includes a storage cavity 401, the storage cavity 401 is opened inside the storage box 1, a storage drawer 402 is movably inserted into the storage cavity 401, a first sealing plate 403 is arranged on a side wall of the storage drawer 402, and when the storage drawer 402 and the first sealing plate 403 are closed, the first sealing plate 403 is flush with the side wall of the storage box 1; specifically, after sampling, the storage drawer 402 is pulled out from the inside of the storage cavity 401 by pulling the first sealing plate 403, the sampled sample is placed in the storage drawer 402, and the storage drawer 402 enters the storage cavity 401 by pushing the first sealing plate 403, so that the sample is stored.
As shown in fig. 1, the operating platform assembly 5 includes a placing groove 501, the placing groove 501 is formed in the front side wall of the storage box 1, an operating table plate 502 is hinged to the left and right side walls of the placing groove 501, a handle groove 503 is formed in the bottom of the operating table plate 502, a connecting rope 504 is fixedly installed on the left and right side walls of the placing groove 501, the free end of the connecting rope 504 is fixedly installed on the top of the operating table plate 502 in an inserting manner, and the connecting rope 504 is made of a flexible material; specifically, pulling operation table 502 forward through handle slot 503 for operation table 502 is perpendicular setting with standing groove 501 under the effect of connecting rope 504, and then is convenient for handle the sample time convenient operation, uses the completion back, makes operation table 502 overturn to standing groove 501's inside and then withdraws operation table 502 through pushing operation table 502, is convenient for accomodate.
As shown in fig. 1 and 2, the storage mechanism 7 includes a storage cavity 701, a storage cavity 701 is opened on a front side wall of the side box 6, a storage box 702 is movably disposed in the storage cavity 701, and a second sealing plate 703 is fixedly mounted on the front side wall of the storage box 702; specifically, the storage box 702 is convenient for storing tools during detection.
As shown in fig. 3 and 4, the heating mechanism 8 includes a heating cavity 801, the top of the side box 6 is provided with the heating cavity 801, a heating plate 802 is attached to the surface of the heating cavity 801, a second test tube rack 803 is fixedly mounted on the inner wall of the heating plate 802, and a second test tube fixing through hole 804 is provided at the top of the second test tube rack 803; specifically, the test tube that will have the medicament is placed inside second test tube fixing through hole 804, will heat cavity 801's internal seal through second lid 9, and then realize heating cavity 801's inside through hot plate 802 to be convenient for heat the test tube.
As shown in figures 1 to 4, the application method of the pesticide residue rapid detection kit comprises the following steps:
s1: sampling; when sampling, samples from different plant leaves (at least one to eight leaves) are taken by a stainless steel tube sampler, fruits and vegetables are sampled from the skin to the pulp at a position of one to one point and five centimeters, and the sampled fruit and vegetable samples are stored in a sample storage mechanism 4;
s2: processing a sample; taking out the sample stored in the sample storage mechanism 4, unfolding the operation table assembly 5, placing the sample on the operation table assembly 5, cutting the sample into pieces through props, taking out the test tube from the storage mechanism 2, and adding the cut sample into four grams of the test tube;
s3: extracting a sample extracting solution; adding twenty milliliters of buffer solution into a test tube with a sample, manually shaking for two minutes, pouring the extracting solution into a small test tube for three milliliters, and standing the small test tube for three to five minutes;
s4: detecting; fifty microliters of butyrylcholinesterase and fifty microliters of color-developing agent are added into a small test tube in which the extracting solution is stored, the small test tube is placed into a heating mechanism 8, placed for five minutes at a constant temperature of thirty-eight degrees, then fifty microliters of substrate is added, the mixture is poured into a cuvette, and the mixture is vibrated and measured by using a rapid pesticide residue measuring instrument.
As shown in fig. 1-4; in step S4, the small test tubes are rapidly mixed when the substrate is added.
Claims (8)
1. The utility model provides a quick detect reagent box of pesticide residue, its characterized in that, includes case (1), the top of case (1) is provided with receiving mechanism (2) that are used for depositing the test tube and detect the medicament, articulated first lid (3) of installing in the top of case (1), a lateral wall of case (1) is provided with sample storage mechanism (4), the preceding lateral wall of case (1) is provided with and is used for operation when detecting pesticide residue operation platform subassembly (5), another lateral wall of case (1) who has side box (6), the preceding lateral wall of side box (6) is provided with and is used for that pesticide residue detection utensil carries out what deposit thing mechanism (7), the top of side box (6) is provided with heating mechanism (8), the articulated second lid (9) of installing in the top of side box (6), case (1) a lateral wall articulates with another lateral wall of side box (6) and installs handle (10).
2. The rapid pesticide residue detection kit according to claim 1, wherein the storage mechanism (2) comprises a test tube fixing base (201), the test tube fixing base (201) is fixedly mounted at the bottom of the inner side of the storage box (1), a fixing groove (202) is formed in the top of the test tube fixing base (201), a first test tube rack (203) is fixedly mounted on the inner wall of the storage box (1), a first test tube fixing through hole (204) is formed in the top of the first test tube rack (203), the first test tube fixing through hole (204) and the fixing groove (202) are in multiple groups, and the multiple groups of first test tube fixing through holes (204) and the fixing groove (202) are correspondingly arranged.
3. The pesticide residue rapid detection kit according to claim 1, wherein the sample storage mechanism (4) comprises a storage cavity (401), the storage cavity (401) is formed inside the storage box (1), a storage drawer (402) is movably inserted into the storage cavity (401), a first sealing plate (403) is arranged on one side wall of the storage drawer (402), and when the storage drawer (402) and the first sealing plate (403) are closed, the first sealing plate (403) is flush with one side wall of the storage box (1).
4. The pesticide residue rapid detection kit according to claim 1, wherein the operation table component (5) comprises a placement groove (501), the placement groove (501) is formed in the front side wall of the storage box (1), operation table plates (502) are hinged to the left side wall and the right side wall of the placement groove (501), a handle groove (503) is formed in the bottom of the operation table plates (502), connection ropes (504) are fixedly installed on the left side wall and the right side wall of the placement groove (501), the free ends of the connection ropes (504) are fixedly installed on the top of the operation table plates (502) in an inserted manner, and the connection ropes (504) are made of flexible materials.
5. The pesticide residue rapid detection kit according to claim 1, wherein the storage mechanism (7) comprises a storage cavity (701), the storage cavity (701) is formed in the front side wall of the side box (6), the storage box (702) is movably arranged in the storage cavity (701), and a second sealing plate (703) is fixedly arranged on the front side wall of the storage box (702).
6. The pesticide residue rapid detection kit according to claim 1, wherein the heating mechanism (8) comprises a heating cavity (801), the top of the side box (6) is provided with the heating cavity (801), the surface of the heating cavity (801) is attached with a heating plate (802), the inner wall of the heating plate (802) is fixedly provided with a second test tube rack (803), and the top of the second test tube rack (803) is provided with a second test tube fixing through hole (804).
7. The use method of the pesticide residue rapid detection kit according to any one of claims 1 to 6, characterized by comprising the following steps:
s1: sampling; when sampling, samples from different plant leaves (at least one to eight leaves) are taken by a stainless steel tube sampler, fruits and vegetables are sampled from the skin to the pulp at a position of one to one point and five centimeters, and the sampled fruit and vegetable samples are stored in a sample storage mechanism (4);
s2: processing a sample; taking out the sample stored in the sample storage mechanism (4), unfolding the operating table assembly (5), placing the sample on the operating table assembly (5), chopping the sample through props, taking out a test tube from the storage mechanism (2), and adding the chopped sample into the test tube by four grams;
s3: extracting a sample extracting solution; adding twenty milliliters of buffer solution into a test tube with a sample, manually shaking for two minutes, pouring the extracting solution into a small test tube for three milliliters, and standing the small test tube for three to five minutes;
s4: detecting; fifty microliters of butyrylcholine esterase and fifty microliters of color developing agent are added into a small test tube in which the extracting solution is stored, the small test tube is placed into a heating mechanism (8) and placed at a constant temperature of thirty-eight ℃ for five minutes, then fifty microliters of substrate is added, the small test tube is poured into a cuvette, and the cuvette is vibrated and then measured by using a pesticide residue rapid measuring instrument.
8. The use method of the rapid detection kit for pesticide residues according to claim 7, characterized in that in step S4, the small test tubes are rapidly mixed when the substrate is added.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211074253.4A CN115465572A (en) | 2022-09-02 | 2022-09-02 | Rapid detection kit for pesticide residues and use method thereof |
Applications Claiming Priority (1)
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| CN203624221U (en) * | 2014-01-07 | 2014-06-04 | 王俊凤 | Portable temporary sample storage box for use in food detection |
| CN105259276A (en) * | 2014-07-18 | 2016-01-20 | 农业药物毒物试验所 | Rapid extraction kit for agricultural product pesticide residue detection program and method for obtaining detection liquid stock solution from agricultural product sample |
| CN206476981U (en) * | 2017-01-10 | 2017-09-08 | 武汉赛云博生物科技有限公司 | A kind of kit detected for B cell leukemia minimal residual disease |
| CN212083239U (en) * | 2020-03-26 | 2020-12-04 | 山西中谱安信质检技术服务有限公司 | Pesticide residue detection case convenient to use |
| CN214844386U (en) * | 2020-12-10 | 2021-11-23 | 南京启迪环保新能源有限公司 | Be used for pesticide residue short-term test preliminary treatment box |
| CN215206003U (en) * | 2021-05-12 | 2021-12-17 | 深圳康生保生物技术有限公司 | Novel estradiol determination kit |
| CN216525671U (en) * | 2021-11-15 | 2022-05-13 | 甄研科技(上海)有限公司 | Food additive detector |
| CN217199781U (en) * | 2022-02-21 | 2022-08-16 | 崔宪红 | Test tube strorage device for medical science inspection |
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|---|---|---|---|---|
| CN203624221U (en) * | 2014-01-07 | 2014-06-04 | 王俊凤 | Portable temporary sample storage box for use in food detection |
| CN105259276A (en) * | 2014-07-18 | 2016-01-20 | 农业药物毒物试验所 | Rapid extraction kit for agricultural product pesticide residue detection program and method for obtaining detection liquid stock solution from agricultural product sample |
| US20160018305A1 (en) * | 2014-07-18 | 2016-01-21 | Agricultural Chemicals And Toxic Substances Research Institute, Council Of Agriculture | Quick extraction kit adapted to a procedure of detecting pesticide residues in agricultural products and a method of obtaining a primary test liquid from an agricultural sample by the quick extraction kit |
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| CN212083239U (en) * | 2020-03-26 | 2020-12-04 | 山西中谱安信质检技术服务有限公司 | Pesticide residue detection case convenient to use |
| CN214844386U (en) * | 2020-12-10 | 2021-11-23 | 南京启迪环保新能源有限公司 | Be used for pesticide residue short-term test preliminary treatment box |
| CN215206003U (en) * | 2021-05-12 | 2021-12-17 | 深圳康生保生物技术有限公司 | Novel estradiol determination kit |
| CN216525671U (en) * | 2021-11-15 | 2022-05-13 | 甄研科技(上海)有限公司 | Food additive detector |
| CN217199781U (en) * | 2022-02-21 | 2022-08-16 | 崔宪红 | Test tube strorage device for medical science inspection |
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