CN114735358B

CN114735358B - Closed and sealed delivery system used after sampling and used for TRPC6 nucleic acid amplification detection

Info

Publication number: CN114735358B
Application number: CN202210226757.7A
Authority: CN
Inventors: 闫锦锦; 桂宁; 刘冬; 王明
Original assignee: Wuhu Maozhi Biotechnology Co ltd
Current assignee: Hangzhou Maozhi Biotechnology Co ltd; Wuhu Maozhi Biotechnology Co ltd
Priority date: 2022-03-09
Filing date: 2022-03-09
Publication date: 2022-09-30
Anticipated expiration: 2042-03-09
Also published as: CN114735358A

Abstract

The invention discloses a sealed conveying system after sampling for TRPC6 nucleic acid amplification detection, which comprises a conveying box, wherein a sealing strip is fixedly arranged in the conveying box, a sealing cover is rotatably arranged on the conveying box, a heat-insulating layer is fixedly arranged on the sealing cover, a base is fixedly arranged in the conveying box, a supporting gear is rotatably arranged on the base, a transfer rack is meshed with the supporting gear, a sealed kit structure is arranged on the transfer rack through a socket with a clamping block, a kit cover opening structure is arranged in the conveying box, the kit cover opening structure is connected with an inhibitor dripping structure through a pull rope, and the pull rope is movably arranged in a steering cable sleeve; according to the closed conveying system, the conveying box with the sealing and freezing mechanisms is used as a storage and conveying device for the RNA samples, inhibitor addition is not needed at indoor temperature, the operation time difference is effectively eliminated, the samples are guaranteed to be always in a low-temperature environment, and degradation is avoided.

Description

Closed and sealed delivery system used after sampling and used for TRPC6 nucleic acid amplification detection

Technical Field

The invention relates to the technical field of medical detection, in particular to a closed and sealed conveying system after sampling for TRPC6 nucleic acid amplification detection.

Background

Alzheimer's disease is a progressive degenerative disease of the nervous system with occult onset. Clinically, the overall dementia manifestations such as memory disorder, aphasia, disuse, agnosia, visual space skill impairment, executive dysfunction and personality and behavior change are characterized, and the diagnosis is mainly performed by multiple means such as medical history, symptoms and signs, neuropsychological test, body fluid detection, neuroimaging examination, electrophysiological detection and gene detection. Studies have shown that TRPC6 reduces the formation of the neurotoxic molecule Amyloid beta (Α β) in alzheimer's disease by interfering with the binding of Amyloid beta precursor (APP) to gamma secretase in the human brain. In addition, in the peripheral blood of AD patients, the level of TRPC6 gene transcription mRNA is reduced, so the content change of TRPC6 mRNA in the peripheral blood can be detected to play a role in auxiliary diagnosis of AD.

At present, when detection is carried out, a blood RNA extraction reagent device is firstly adopted to extract a total RNA sample in human blood leucocytes, and then the RNA sample is sent for detection. The single-stranded structure of RNA samples is fragile and is easily affected by temperature and degradation by RNase. Therefore, the sample is required to be stored at the temperature of-70 ℃ or below, the RNA enzyme pollution is reduced, the degradation is delayed, 1U/ul of RNA enzyme inhibitor is added into the extracted RNA sample, the degradation of the RNA enzyme can be effectively inhibited, and the low-temperature long-term storage is facilitated. However, in the operation process, after the RNA sample is extracted, the enzyme inhibitor is usually required to be added dropwise at room temperature, a time difference exists between the steps of extraction and low-temperature storage, so that the sample is easily degraded, and the existing conveying system for RNA sample storage and transportation has the problems of slow temperature reduction and uneven cooling, so that the time for the sample to reach the low temperature is long, and the degradation of the sample is further aggravated.

Disclosure of Invention

The invention aims to provide a closed and sealed delivery system after sampling for TRPC6 nucleic acid amplification detection, so as to achieve the purposes of eliminating the operation time difference of inhibitor addition and avoiding sample degradation, and solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a sampling rear airtight sealed conveying system for TRPC6 nucleic acid amplification detection comprises a conveying box, wherein a sealing strip is fixedly mounted in the conveying box, a sealing cover is rotatably mounted on the conveying box, a heat preservation layer is fixedly mounted on the sealing cover, a refrigerator is fixedly mounted in the conveying box, an air supply opening is formed in the refrigerator, a base is fixedly mounted in the conveying box, a supporting gear is rotatably mounted on the base, a transfer rack is meshed on the supporting gear and is of a runner type connected end to end, an airtight kit structure with a pressure opening function is mounted on the transfer rack through a socket with a fixture block, the supporting gear is driven through a driving structure with an one-way gap driving function, a kit cover opening structure with an avoiding function is mounted in the conveying box, and the kit cover opening structure is connected with an inhibitor dropwise adding structure with an automatic adding function through a pull rope, and the pull rope is movably arranged in the steering cable sleeve.

Preferably, the closed kit structure comprises an extraction kit inserted into the socket, a positioning groove is formed in the extraction kit, a closed cover is rotatably mounted on the extraction kit, an end block is fixedly connected to the closed cover, a hanging rod is rotatably mounted on the end block, and a ball is arranged at the bottom of the hanging rod.

Preferably, the extraction kit adopts a nucleic acid purification kit, the positioning groove is formed in the side face of the extraction kit and connected with the clamping block, the closed cover is installed through a hinge with a torsion spring, the end block is arranged at the front end of the closed cover, and the hanging rod is suspended on the side face of the extraction kit.

Preferably, the driving structure comprises a driving seat fixedly mounted on the base, a crank disc is rotatably mounted on the driving seat, a connecting rod is rotatably mounted on the crank disc and is rotatably connected to the sliding block, the sliding block is slidably mounted on the guide rod, a driving plate is fixedly connected to the sliding block, and one-way oblique teeth are fixedly mounted on the driving plate.

Preferably, the driving seat is installed on the top surface of the base, a speed reduction motor is arranged in the driving seat, the crank disc is connected to the driving shaft, the crank disc, the connecting rod and the sliding block form a crank-sliding block structure, the driving plate is vertically connected with the sliding block, and the unidirectional oblique teeth are arranged on the side face of the driving plate.

Preferably, the reagent box structure of uncapping is including the first spring of fixed connection on the base, and first spring fixed connection on the slide, slide fixed connection is on starting the push rod, and fixedly connected with lifting plate on the slide, fixed mounting has the cambered surface seat on the lifting plate, slide slidable mounting is in the bottom of transporting the case, and starts the push rod and be electric push rod, the top surface of cambered surface seat sets up to the arcwall face, the reagent box structure of uncapping is close to the turn of shifting the rack and installs.

Preferably, turn to the cable cover and install on the inner wall that transports the case, and turn to and be provided with the grooving in the cable cover, inhibitor dropwise add structure is including the inhibitor box of being connected with the stay cord, and inhibitor box slidable mounting is on the slide, fixed mounting has the second spring in the slide, and is provided with on the inhibitor box and adds sprue and liquid outlet, fixed mounting has the burette on the liquid outlet, and is provided with the sealing plug in the burette, and is connected with press switch on the sealing plug.

Preferably, the inhibitor box is filled with 1U/ul RNase inhibitor, the inhibitor box is slidably mounted in the slideway through a limiting block, the limiting block is connected with a second spring, the liquid outlet is arranged on the side surface of the inhibitor box, and the nozzle of the dropper faces downwards vertically.

Preferably, the sealing strip is installed around the inner wall of transporting the case, and the heat preservation is installed on the inner wall of sealed lid, the refrigerator is the frostless refrigerator of forced air cooling, two are installed at base top symmetry through the shaft to the support gear, and transfer the rack and connect between two support gears around rolling up.

Compared with the prior art, the invention has the beneficial effects that:

1. the closed conveying system of the invention utilizes the conveying box with the sealing and freezing mechanism as a storage and transportation device of RNA samples, the conveying box is an effective low-temperature heat insulation structure, the conveying box also utilizes a runway type transfer rack to form a circulating structure, the positions of closed reagent kits containing the RNA samples can be continuously changed, all the arranged closed reagent kit structures pass through the air outlet in turn to achieve the effect of uniformly cooling the RNA samples, furthermore, the closed conveying system can also complete the work of automatically adding inhibitors in the box, after the extraction reagent kits with the RNA samples are placed in the conveying box, the continuous position transfer of the samples can be simply carried out through the transfer rack to achieve the effect of uniformly cooling, the cover opening structure of the reagent kit can also be utilized to open the closed cover of the extraction reagent kit moved to a turning point, and simultaneously, the dropping structure of the inhibitors is utilized, the inhibitor enters the extraction kit, and the inhibitor does not need to be added independently at indoor temperature, so that the operation time difference is effectively eliminated, the sample is ensured to be always in a low-temperature environment, and degradation is avoided;

2. the movement of the extraction kit is achieved through the driving structure, the driving structure has the characteristic of one-way intermittent driving, so that the extraction kit can temporarily stop at the air outlet and the inhibitor dripping structure to complete rapid cooling and inhibitor adding work, the inhibitor dripping structure is connected with the kit cover opening structure through the pull rope, the extraction kit and the inhibitor dripping structure are in linkage and synchronously work, when the kit cover opening structure is in avoidance, the inhibitor dripping structure is also in an avoidance state, and two working modes (an inhibitor adding mode and a uniform refrigerating mode) of the conveying box can stably work.

Drawings

FIG. 1 is a first schematic view of the shipping box structure of the present invention;

FIG. 2 is a second schematic view of the shipping box structure of the present invention;

FIG. 3 is a third schematic view of the shipping box structure of the present invention;

FIG. 4 is a schematic diagram of a sample storage structure according to the present invention;

FIG. 5 is a schematic view of an intermittent drive configuration of the present invention;

FIG. 6 is a schematic view of a sample transfer structure according to the present invention;

FIG. 7 is an enlarged view of area A in FIG. 6;

FIG. 8 is a schematic view of an automatic inhibitor addition structure according to the present invention;

FIG. 9 is a schematic view of the lid opening structure of the present invention;

FIG. 10 is a schematic view showing a dropping structure of an inhibitor of the present invention.

In the figure: the device comprises a conveying box 1, a sealing strip 2, a sealing cover 3, a heat-insulating layer 4, a refrigerator 5, an air supply opening 6, a base 7, a supporting gear 8, a transfer rack 9, a socket 10, a fixture block 11, an extraction kit 12, a positioning groove 13, a sealing cover 14, an end block 15, a hanging rod 16, a driving seat 17, a crank disc 18, a connecting rod 19, a sliding block 20, a guide rod 21, a driving plate 22, a one-way inclined tooth 23, a first spring 24, a sliding seat 25, a starting push rod 26, a lifting plate 27, a cambered surface seat 28, a pull rope 29, a steering cable sleeve 30, a restraining kit 31, a slide way 32, a second spring 33, an injection opening 34, a liquid outlet 35, a dropper 36 and a press switch 37.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and the detailed description, wherein various embodiments or technical features described below can be arbitrarily combined to form a new embodiment without conflict, and it should be understood that the described embodiments are only a part of embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 to 10, the present invention provides a technical solution: a closed and sealed delivery system after sampling for TRPC6 nucleic acid amplification detection comprises a delivery box 1, and is characterized in that: the conveying box 1 is fixedly provided with a sealing strip 2, the conveying box 1 is rotatably provided with a sealing cover 3, the sealing cover 3 is fixedly provided with a heat preservation layer 4, the conveying box 1 is fixedly provided with a refrigerator 5, the refrigerator 5 is provided with an air supply outlet 6, the conveying box 1 is fixedly provided with a base 7, the base 7 is rotatably provided with a supporting gear 8, the supporting gear 8 is engaged with a transfer rack 9, the transfer rack 9 is of an end-to-end runway type, the transfer rack 9 is provided with a closed kit structure with a pressure opening function through a socket 10 with a clamping block 11, the supporting gear 8 is driven through a driving structure with a one-way gap driving function, the conveying box 1 is provided with a kit cover opening structure with an avoiding function, and the kit cover opening structure is connected with an inhibitor dripping structure with an automatic adding function through a pull rope 29, and the pull rope 29 is movably mounted in the steering cable cover 30.

The sealing strips 2 are arranged on the periphery of the inner wall of the conveying box 1, the heat-insulating layer 4 is arranged on the inner wall of the sealing cover 3, the refrigerator 5 is an air-cooled frost-free refrigerator, two supporting gears 8 are symmetrically arranged on the top of the base 7 through wheel shafts, and the transfer rack 9 is connected between the two supporting gears 8 in a winding manner;

the conveying box 1 with a sealing and freezing mechanism is used as a storage and transportation device of RNA samples, the sealing strip 2 is arranged in the conveying box 1, the heat-insulating layer 4 is arranged on the sealing cover 3, when the sealing cover 3 is covered, the heat-insulating layer 4 is tightly connected with the sealing strip 2, so that the conveying box 1 can form an effective heat-insulating structure, the conveying box 1 is internally provided with the air-cooled refrigerator 5, the air outlet 6 is used for generating cold air, the temperature in the box is quickly reduced to be below 70 ℃, and the frosting is not easy to occur;

the closed kit structure comprises an extraction kit 12 inserted in the socket 10, a positioning groove 13 is arranged on the extraction kit 12, a closed cover 14 is rotatably arranged on the extraction kit 12, an end block 15 is fixedly connected to the closed cover 14, a suspender 16 is rotatably arranged on the end block 15, and a ball is arranged at the bottom of the suspender 16;

the conveying box 1 is also provided with a supporting gear 8 through a base 7, so that a circulating structure is formed by the transfer rack 9 in the conveying box 1, the outer wall of the transfer rack 9 is provided with the sockets 10 in an arranging manner, the sockets 10 are used for quickly installing the closed kit structure, when the transfer rack 9 rotates, the position of the closed kit structure can be changed, all the arranged closed kit structures pass through the air outlet 6 in turn, and the effect of uniformly cooling RNA samples is achieved;

the extraction kit 12 adopts a nucleic acid purification kit, the positioning groove 13 is arranged on the side surface of the extraction kit 12 and is connected with the fixture block 11, the closed cover 14 is installed through a hinge with a torsion spring, the end block 15 is arranged at the front end of the closed cover 14, and the suspender 16 is suspended on the side surface of the extraction kit 12;

the main body of the closed kit structure is an extraction kit 12, which can perform high-quality purification extraction of RNA samples of blood leukocytes, the extraction kit 12 is directly inserted into a socket 10 after extraction, rotary uniform low-temperature preservation is directly performed in a transport box 1, a closed cover 14 is further arranged on the extraction kit 12, the closed cover 14 can be opened through stress of a suspender 16 in the rotary process of the extraction kit 12, automatic in-box inhibitor dripping operation is performed, and the RNA samples in the extraction kit 12 are guaranteed against degradation;

the driving structure comprises a driving seat 17 fixedly installed on the base 7, a crank disc 18 is installed on the driving seat 17 in a rotating mode, a connecting rod 19 is installed on the crank disc 18 in a rotating mode, the connecting rod 19 is connected to a sliding block 20 in a rotating mode, the sliding block 20 is installed on a guide rod 21 in a sliding mode, a driving plate 22 is fixedly connected to the sliding block 20, and one-way inclined teeth 23 are fixedly installed on the driving plate 22;

the movement of the transfer rack 9 and the extraction kit 12 thereon is achieved through a driving structure, and the driving structure has the characteristic of one-way intermittent driving, so that the extraction kit 12 can temporarily stay at the air outlet 6 and the inhibitor dripping structure, and the rapid cooling and inhibitor adding work is completed;

the driving seat 17 is installed on the top surface of the base 7, a speed reduction motor is arranged in the driving seat 17, the crank disk 18 is connected to the driving shaft, the crank disk 18, the connecting rod 19 and the sliding block 20 form a crank sliding block structure, the driving plate 22 is vertically connected with the sliding block 20, and the unidirectional helical teeth 23 are arranged on the side surface of the driving plate 22;

during driving, the crank disc 18 rotates to drive the sliding block 20 to slide on the guide rod 21 in a reciprocating manner, the one-way helical teeth 23 mounted on the sliding block 20 through the driving plate 22 can be meshed with the supporting gear 8, so that the supporting gear 8 rotates to further shift the position of the extraction kit 12, and when the one-way helical teeth 23 reset, the one-way helical teeth can slide relative to the supporting gear 8, so that one-way driving is performed;

the cover opening structure of the kit comprises a first spring 24 fixedly connected to the base 7, the first spring 24 is fixedly connected to a sliding seat 25, the sliding seat 25 is fixedly connected to a starting push rod 26, a lifting plate 27 is fixedly connected to the sliding seat 25, a cambered surface seat 28 is fixedly mounted on the lifting plate 27, the sliding seat 25 is slidably mounted at the bottom of the conveying box 1, the starting push rod 26 is an electric push rod, the top surface of the cambered surface seat 28 is set to be a cambered surface, and the cover opening structure of the kit is mounted close to the turning position of the transfer rack 9;

after the extraction kit 12 with the RNA sample is placed in the conveying box 1, the continuous position transfer of the sample can be simply carried out through the transfer rack 9, the effect of uniform cooling is achieved, the cover opening structure of the kit can also be utilized, the sealing cover 14 of the extraction kit 12 moved to the turning point is opened, meanwhile, the sealing cover 1 is utilized to act on the inhibitor dripping structure, so that the inhibitor enters the extraction kit 12, the automatic in-box dripping operation is completed, the inhibitor addition does not need to be carried out independently at indoor temperature, the sample is ensured to be always in a low-temperature environment, and the degradation is avoided;

the steering cable sleeve 30 is installed on the inner wall of the conveying box 1, a rope groove is formed in the steering cable sleeve 30, the inhibitor dripping structure comprises an inhibitor box 31 connected with the pull rope 29, the inhibitor box 31 is installed on a slide way 32 in a sliding mode, a second spring 33 is fixedly installed in the slide way 32, an injection port 34 and a liquid outlet 35 are formed in the inhibitor box 31, a dropper 36 is fixedly installed on the liquid outlet 35, a sealing plug is arranged in the dropper 36, and a press switch 37 is connected to the sealing plug;

the inhibitor dripping structure is connected with the kit cover opening structure through a pull rope 29, the inhibitor dripping structure and the kit cover opening structure are linked and work simultaneously, and when the kit cover opening structure avoids, the inhibitor dripping structure also keeps an avoiding state;

1U/ul RNase inhibitor is contained in the inhibitor box 31, the inhibitor box 31 is slidably mounted in the slideway 32 through a limiting block, the limiting block is connected with the second spring 33, the liquid outlet 35 is arranged on the side surface of the inhibitor box 31, and the nozzle of the dropper 36 faces vertically downwards;

when the closed cover 14 on the extraction reagent box 12 is opened, pressure is applied to the press switch 37, the dropper 36 is opened, so that the inhibitor in the inhibitor box 31 enters the extraction reagent box 12 from the dropper 36, and the automatic reagent box adding work is completed;

the low temperature and the sterile environment in the transport box 1 are ensured, because the whole inhibitor adding work is carried out in the sterile low temperature environment, the extraction kit 12 is not required to be worried about the bacteria being doped, the temperature can be directly reduced after the sample is extracted, the temperature reduction and the inhibitor adding work are carried out synchronously, the time of the sample at the unsuitable temperature is effectively reduced, the sample degradation degree is greatly reduced, when in use, the RNA sample in the extraction kit is detected by utilizing the TRPC6 nucleic acid amplification detection kit, the detection kit adopts the total RNA extracted from peripheral blood leucocytes in one-step fluorescence quantitative PCR as a template, and utilizes upstream and downstream primers specific to a target gene TRPC6 and an internal reference gene Actin to realize the amplification of TRPC6 and Actin genes in one-step method, and combines a statistical analysis method to analyze the ratio of the transcription mRNA levels of the TRPC6 and the Actin genes in the peripheral blood of diagnosed AD patients and non-AD patients, the detection can be finished;

when the invention is used: firstly, the invention uses a conveying box 1 with a sealing and freezing mechanism as a device for storing and transporting RNA samples, a sealing strip 2 is arranged in the conveying box 1, a heat-insulating layer 4 is arranged on a sealing cover 3, when the sealing cover 3 is covered, the heat-insulating layer 4 is tightly connected with the sealing strip 2, so that the conveying box 1 can form an effective heat-insulating structure, an air-cooled refrigerator 5 is arranged in the conveying box 1, air is generated by an air outlet 6, the temperature in the box is quickly reduced to be below 70 ℃, frosting is not easy to occur, the conveying box 1 is also provided with a supporting gear 8 through a base 7, so that a circulating structure is formed by a transfer rack 9 in the conveying box 1, the arrangement and installation of sockets 10 are carried out on the outer wall of the transfer rack 9, the sockets 10 carry out the quick installation of a closed reagent box structure, and when the transfer rack 9 rotates, the position of the closed reagent box structure can be changed, all the arranged closed reagent kit structures pass through the air outlet 6 in turn to achieve the effect of uniformly cooling RNA samples, the main body of the closed reagent kit structure is an extraction reagent kit 12 which can perform high-quality purification extraction on the RNA samples of blood leukocytes, the extraction reagent kit 12 is directly inserted into a socket 10 after extraction, rotary uniform low-temperature preservation is directly performed in the conveying box 1, a closed cover 14 is further arranged on the extraction reagent kit 12, the closed cover 14 can be opened through the stress of a suspender 16 in the rotary process of the extraction reagent kit 12 to perform automatic in-box inhibitor dripping operation, the RNA samples in the extraction reagent kit 12 are guaranteed not to be degraded, the movement of the transfer rack 9 and the extraction reagent kit 12 thereon is achieved through a driving structure, and the driving structure has the characteristic of unidirectional intermittent driving, so that the extraction reagent kit 12 can temporarily stop at the air outlet 6 and the inhibitor dripping structure, finishing the work of rapid cooling and inhibitor addition, during driving, the crank disc 18 rotates to drive the slide block 20 to slide on the guide rod 21 in a reciprocating manner, the one-way helical teeth 23 arranged on the slide block 20 by using the driving plate 22 can be meshed with the supporting gear 8, so that the supporting gear 8 rotates to further transfer the position of the extraction kit 12, and when the one-way helical teeth 23 reset, the one-way helical teeth can slide relative to the supporting gear 8, so that one-way driving is performed;

after the extraction kit 12 with the RNA sample is placed in the conveying box 1, the continuous position transfer of the sample can be simply carried out through the transfer rack 9 to achieve the effect of uniform cooling, the opening structure of the kit can also be utilized to open the closed cover 14 of the extraction kit 12 moved to the turning point, meanwhile, the closed cover 1 is utilized to act on the inhibitor dripping structure, so that the inhibitor enters the extraction kit 12 to complete the automatic in-box dripping operation, the inhibitor is not required to be added independently at indoor temperature, the sample is ensured to be always in a low-temperature environment and prevented from being degraded, the inhibitor dripping structure is connected with the opening structure of the kit through a pull rope 29, the inhibitor and the opening structure are linked and work simultaneously, when the opening structure of the kit is avoided, the inhibitor dripping structure also keeps an avoiding state, and particularly, when the closed cover 14 on the extraction kit 12 is opened, pressure is applied to the push switch 37 and the dropper 36 is opened, so that the inhibitor in the inhibitor box 31 enters the extraction kit 12 from the dropper 36, and automatic kit-by-kit addition work is completed.

The above embodiments are merely preferred embodiments of the present invention, and the scope of the present invention should not be limited thereto, and those skilled in the art will be able to make various modifications without inventive changes from the above concepts and the scope of the present invention.

Claims

A closed and sealed delivery system after sampling for TRPC6 nucleic acid amplification detection, which comprises a delivery box (1) and is characterized in that: the box is characterized in that a sealing strip (2) is fixedly mounted in the conveying box (1), a sealing cover (3) is rotatably mounted on the conveying box (1), a heat preservation layer (4) is fixedly mounted on the sealing cover (3), a refrigerator (5) is fixedly mounted in the conveying box (1), an air supply opening (6) is formed in the refrigerator (5), a base (7) is fixedly mounted in the conveying box (1), a supporting gear (8) is rotatably mounted on the base (7), a transfer rack (9) is meshed on the supporting gear (8), the transfer rack (9) is of an end-to-end runner type, a closed kit structure with a pressure opening function is mounted on the transfer rack (9) through a socket (10) with a clamping block (11), the supporting gear (8) is driven through a driving structure with an one-way gap driving function, and a kit cover opening structure with an avoiding function is mounted in the conveying box (1), the cover opening structure of the kit is connected with an inhibitor dripping structure with an automatic adding function through a pull rope (29), and the pull rope (29) is movably arranged in a steering cable sleeve (30);

the closed kit structure comprises an extraction kit (12) inserted in the socket (10), a positioning groove (13) is formed in the extraction kit (12), a closed cover (14) is rotatably mounted on the extraction kit (12), an end block (15) is fixedly connected to the closed cover (14), a hanging rod (16) is rotatably mounted on the end block (15), and a round ball is arranged at the bottom of the hanging rod (16); the driving structure comprises a driving seat (17) fixedly mounted on a base (7), a crank disc (18) is rotatably mounted on the driving seat (17), a connecting rod (19) is rotatably mounted on the crank disc (18), the connecting rod (19) is rotatably connected to a sliding block (20), the sliding block (20) is slidably mounted on a guide rod (21), a driving plate (22) is fixedly connected to the sliding block (20), and unidirectional oblique teeth (23) are fixedly mounted on the driving plate (22); the cover opening structure of the kit comprises a first spring (24) fixedly connected to a base (7), the first spring (24) is fixedly connected to a sliding seat (25), the sliding seat (25) is fixedly connected to a starting push rod (26), a lifting plate (27) is fixedly connected to the sliding seat (25), an arc surface seat (28) is fixedly mounted on the lifting plate (27), the sliding seat (25) is slidably mounted at the bottom of a conveying box (1), the starting push rod (26) is an electric push rod, the top surface of the arc surface seat (28) is arranged to be an arc surface, and the cover opening structure of the kit is mounted close to a turning part of a transfer rack (9); the steering cable sleeve (30) is installed on the inner wall of the conveying box (1), a rope groove is formed in the steering cable sleeve (30), the inhibitor dripping structure comprises an inhibitor box (31) connected with a pull rope (29), the inhibitor box (31) is installed on a slide way (32) in a sliding mode, a second spring (33) is fixedly installed in the slide way (32), an injection port (34) and a liquid outlet (35) are formed in the inhibitor box (31), a dropper (36) is fixedly installed on the liquid outlet (35), a sealing plug is arranged in the dropper (36), and a press switch (37) is connected to the sealing plug;

the extraction kit (12) adopts a nucleic acid purification kit, the positioning groove (13) is arranged on the side surface of the extraction kit (12) and connected with the clamping block (11), the closed cover (14) is installed through a hinge with a torsion spring, the end block (15) is arranged at the front end of the closed cover (14), and the hanging rod (16) is suspended on the side surface of the extraction kit (12); the driving seat (17) is installed on the top surface of the base (7), a speed reduction motor is arranged in the driving seat (17), the crank disc (18) is connected to the driving shaft, the crank disc (18), the connecting rod (19) and the sliding block (20) form a crank sliding block structure, the driving plate (22) is vertically connected with the sliding block (20), and the unidirectional oblique teeth (23) are arranged on the side surface of the driving plate (22); 1U/ul RNase inhibitor is arranged in the inhibitor box (31), the inhibitor box (31) is slidably mounted in the slideway (32) through a limiting block, the limiting block is connected with a second spring (33), the liquid outlet (35) is arranged on the side surface of the inhibitor box (31), and the nozzle of the dropper (36) is vertically downward.
2. The system for hermetically sealing and transporting a sample after sampling according to claim 1, which is used for the detection of the amplification of the TRPC6 nucleic acid, and which comprises: sealing strip (2) are installed around the inner wall that transports case (1), and heat preservation (4) are installed on the inner wall of sealed lid (3), refrigerator (5) are the air-cooled frostless refrigerator, support gear (8) are installed two at base (7) top symmetry through the shaft, and transfer rack (9) around the connection between two support gear (8).