CN209362538U - A kind of DNA purification work platform - Google Patents

A kind of DNA purification work platform Download PDF

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Publication number
CN209362538U
CN209362538U CN201821810404.7U CN201821810404U CN209362538U CN 209362538 U CN209362538 U CN 209362538U CN 201821810404 U CN201821810404 U CN 201821810404U CN 209362538 U CN209362538 U CN 209362538U
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China
Prior art keywords
ice bag
storage rack
reagent
reagent storage
dna purification
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CN201821810404.7U
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Inventor
鲁亮
秦伏波
汤军芝
张永霞
肖水冰
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Pu Jian Bio (wuhan) Technology Co Ltd
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Pu Jian Bio (wuhan) Technology Co Ltd
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Abstract

The utility model provides a kind of DNA purification work platform, including cylindrical cabinet body, circular support column is set among cabinet body, round operating platform is arranged in cabinet body top, multiple groups flexible clamp piece is set on operating platform for fixed purifying tube fixing device, circular cover body is set above operating platform, and cover side opens up action pane;Reagent storage rack is set below operating platform, ice bag tray is set below reagent storage rack;Operating platform, reagent storage rack and ice bag tray are removably secured on support column, and the reagent after rotation in reagent storage rack can be contacted with ice bag in ice bag tray;Cabinet body is equipped with opening, and the opening of cabinet body is equipped with the cabinet door being slidably connected with cabinet body.The dual function of reagent storage and purification process can be achieved in the DNA purification work platform of the utility model;Sample can be processed in batches and carry out DNA purification, improve working efficiency;Different sample room cross contaminations are prevented using cover;Facilitate and picks and places reagent and replacement ice bag.

Description

A kind of DNA purification work platform
Technical field
The utility model belongs to Bioexperiment equipment technical field, and in particular to a kind of DNA purification work platform.
Background technique
It usually requires that the plurality of reagents such as cleaning solution, eluent are added in DNA purifying pipe in DNA purification experiment, and passes through Repeatedly centrifugation removal sediment is crossed, the higher DNA solution of purity is finally obtained.It usually needs in DNA purifying for different Sample carries out a large amount of purification experiment, and in multiple purification process, required reagent is more, in order to improve conventional efficient, usually needs Batch addition reagent is carried out in purifying pipe to multiple samples.
Existing DNA purification process platform is unable to adjust purifying tube fixing device and operation when carrying out batch addition reagent Angle between personnel is not easy to operator and quick and precisely adds reagent, and does not have to be easy to produce poor pollution between sample.
Reagent needed for existing DNA is purified needs to store at a lower temperature mostly, and DNA purification process is needed in room temperature Lower progress, general DNA purification process platform do not have temp regulating function, cause to carry out high-volume DNA purification experiment in this way When reagent to take process more troublesome.
In addition it when existing purifying pipe carries out centrifugally operated, needs first to take out from purifying tube fixing device, is put into centrifuge tube In, then each centrifuge tube is respectively put into centrifuge and is centrifuged, purifying pipe is removed after being centrifuged again and is fixed on fixation On frame, it is added next step reagent, each purifying pipe, which requires individually repeatedly to be taken out-being put into-process fixed, to be caused Centrifugal process needs to consume the plenty of time, substantially reduces working efficiency.
Chinese patent CN205188264U discloses a kind of DNA purification process box: box body is cuboid, is behaviour at the top of box body Make platform, station two sides are respectively equipped with left side wall and right side wall, and rear wall is equipped on rear side of station, is divided on left side wall and right side wall It is not fixed with one group of limit chunking, every group of limit chunking includes the first limit block and the second limit block, the first limited block and second Card slot is formed between limited block, station rear wall top is fixed with baffle, multiple through-holes is evenly arranged on baffle, in box body Equipped with box built in two layers, upper layer is kit, and lower layer is ice chest, and kit and ice chest can be to two-way pumpings at left and right sides of box body It draws.The utility model structure is simple, and small volume can simply adjust environment temperature, facilitates operation.But the purification process box Only bottom surface contacts kit with ice chest, and ice chest is slower to the cooling rate of seminal plasma fructose detection kit, and reagent upper temp may be difficult To be maintained at lower temperature;And room temperature is needed in use, needing kit and ice chest all reaching workbench from two side-pullings Outside could take out ice cube, and use has inconvenience more.The kit is not suitable for use in high-volume DNA purification process, different Cross contamination is easy to produce between sample, centrifugal efficiency is relatively low.
Summary of the invention
In order to overcome the deficiencies of the prior art, one of the technical issues of the utility model aims to solve at least in the prior art, A kind of DNA purification work platform is provided, high-volume DNA purification process, and adjustable environment temperature are applicable in, realizes reagent storage and pure Change the dual function of operation.
The utility model is achieved through the following technical solutions: a kind of DNA purification work platform, the cabinet body including cylinder, Circular support column is set among cabinet body, and round operating platform is arranged in cabinet body top, and setting multiple groups flexible clamp piece is used on operating platform Tube fixing device is purified in fixed, circular cover body is set above operating platform, cover side opens up action pane;Below operating platform Reagent storage rack is set, ice bag tray is set below reagent storage rack;Operating platform, reagent storage rack and ice bag tray are equal It is removably secured on support column, the reagent after rotation in reagent storage rack can be contacted with ice bag in ice bag tray;Cabinet body Opening is equipped with for accessing article from reagent storage rack and ice bag tray, the opening of cabinet body, which is equipped with to slide with cabinet body, to be connected The cabinet door connect.
Rotatable operating platform is designed in the DNA purification work platform of the utility model, is arranged on operating platform multiple Tube fixing device is purified, some fixed frame can be made to turn to suitable operating position by rotation, high-volume is facilitated to be purified Experiment improves conventional efficient.Different samples can be placed in different fixed frames when use, certain in workbench is made by rotation One fixed frame is located at below the action pane of circular cover body, is added reagent from action pane, and circular cover body can be to prevent Only when reagent adding, sample prevents sample room cross contamination from splashed upward.The DNA purification work platform of the utility model can store Reagent can also carry out purification process:, can be by reagent storage in reagent storage rack, by rotating reagent when needing to refrigerate reagent Storage rack and ice bag tray make reagent and ice bag contact shut cabinet door and be refrigerated, and when it is desired to be used, open cabinet door, rotation It contacts ice bag and reagent not, and required reagent is rotated into cabinet door opening, facilitate pick-and-place reagent.When needing replacing ice bag When, ice bag tray can also be rotated after enabling, can easily carry out replacement ice bag.
Preferably, the two sides that the cover opens up action pane are equipped with the baffle slided up and down.Baffle is glided can be with It prevents sample during adding reagent from splashing from side, is better protected from cross contamination;When needing rotary operating platform By baffle pull-up, i.e. rotatable operating platform.
Preferably, the fixed frame is circular, multiple purifying pipe fixing grooves is arranged above, and multiple fixing grooves are around fixation It uniformly arranges in a ring in the center of circle of frame;Circular fixed frame can be directly placed into matched centrifugal basket and is centrifuged.It is excellent The purifying pipe with centrifuge tube can be placed on the fixed frame of scheme with Direct Uniform by selecting, and when needing to be centrifuged, not need to take out purifying pipe It puts into a centrifuge, and directly multiple fixed frames can be directly placed into suitable centrifugal basket one by one, balance is put Set, can batch centrifugation, greatly improve working efficiency.
Preferably, the different reagent bottle of multiple groups diameter fixed cave is set in the reagent storage rack, and every group of fixed cave is opposite Support column is uniformly arranged in a ring, and heat shield, reagent bottle is arranged between two neighboring fixed cave in the fixation cave of same group of annular It is put into when being fixed in fixed cave, rotation reagent storage rack can be such that the body of reagent bottle extend between the ice bag of ice bag tray.
It is highly preferred that the ice bag rack of multiple annulars is set on the half of disk of the ice bag tray, it is two neighboring There is interval between annular rack and is used for intercalating agent bottle body.
Ice bag after rotating is facilitated to contact with reagent bottle using the reagent storage rack and ice bag tray of above-mentioned preferred embodiment, when When portion of reagent needs to freeze, it can be deposited by rotating a certain angle or directly take out portion of reagent and not set ice Opposite position, contacts portion of reagent bottle not with ice bag, is arranged between two neighboring fixed cave heat-insulated on the half of disk of bag Piece, which to contact with ice bag, has heat insulation between the reagent bottle that does not contact with ice bag, realize to the portion of reagent of storage into The effect of row refrigeration.The reagent bottle of different-diameter can be placed in reagent storage rack, and each group reagent bottle uniformly arranges and forms annular It is embedded between the ice bag of annular, the contact surface of reagent bottle and ice bag is bigger, and refrigerating speed is fast.
Preferably, the limited block for fixing magnetic frame is also set up on the cover;The jack position of the magnetic frame with Purifying pipe position in fixed frame is oppositely arranged.Corresponding magnetic frame, which is arranged, in this programme can quickly carry out DNA extraction separation.
Preferably, the cabinet body of the operating platform, reagent storage rack, ice bag tray, circular cover body and cylinder is equal It is made of transparent plastic material.Using the more convenient observation inner case of transparent material, facilitate experimental implementation.
Preferably, the operating platform, reagent storage rack and ice bag tray are fixed on support by the positioning ring of lower section On column, positioning ring is fixed on the support columns by clamping screw.The operating platform, reagent storage rack and ice bag tray of this programme It can be needed according to operation and the height of reagent bottle moves up and down adjusting position on the support columns, then use positioning ring and locking Bolt is fixed in suitable height.
It is highly preferred that being equipped with anti-skidding sheet rubber above the positioning ring.The anti-slip rubber piece being arranged on positioning ring can be with Increase frictional force between operating platform, reagent storage rack and ice bag tray and positioning ring, makes its relatively stable fixation, prevent It is arbitrarily rotated, and only when needed, people, which applies certain external force to it, can just make its rotation.
In conclusion the utility model has the advantage that
(1) dual function of reagent storage and purification process can be achieved;And it can realize that the portion of reagent progress to storage is cold The effect of hiding;
(2) sample can be processed in batches and carry out DNA purification, improve working efficiency;
(3) different sample room cross contaminations are prevented;
(4) facilitate observation and experimental implementation, facilitate and pick and place reagent and replacement ice bag;
(5) centrifugal treating can be carried out in batches, improve working efficiency;
(6) height of operating platform, reagent storage rack and ice bag tray is adjustable, adapts to different personnel's operation and difference The reagent bottle of height stores.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of DNA purification work platform of the utility model embodiment 1.
Fig. 2 is operating platform, reagent storage rack and ice inside a kind of DNA purification work platform of the utility model embodiment 1 The structural schematic diagram of bag tray and support column.
Operating platform and support column pass through positioning ring inside a kind of DNA purification work platform of Fig. 3 the utility model embodiment 1 The fixed structural schematic diagram with clamping screw.
Fig. 4 is a kind of structural schematic diagram of DNA purification work platform of the utility model embodiment 2.
In figure: 1, cabinet body;2, support column;3, operating platform;4, fixed frame;5, cover;6, action pane;7, baffle;8, Reagent storage rack;9, ice bag tray;10, it is open;11, cabinet door;12, fixing groove;13, fixed cave;14, heat shield;15, it places Frame;16, magnetic frame;18, positioning ring;19, clamping screw.
Specific embodiment
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, with reference to embodiments, to this Utility model is further elaborated.It should be appreciated that specific embodiment described herein is only used to explain that this is practical It is novel, it is not used to limit the utility model.Based on the embodiments of the present invention, those of ordinary skill in the art are not having Every other embodiment obtained under the premise of creative work is made, is fallen within the protection scope of the utility model.
It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition, term " the One ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
Embodiment 1
As shown in Fig. 1 to 3, circular support is arranged among cabinet body including cylindrical cabinet body 1 in a kind of DNA purification work platform Round operating platform 3 is arranged in column 2,1 top of cabinet body, and setting multiple groups flexible clamp piece is fixed for fixed purifying pipe on operating platform 3 Circular cover body 5 is arranged above operating platform 3 for frame 4, and cover side opens up action pane 6, and the cover 5 opens up action pane Two sides are equipped with the baffle 7 slided up and down;Reagent storage rack 8 is set below operating platform 3, ice is set below reagent storage rack 8 Bag tray 9;Operating platform 3, reagent storage rack 8 and ice bag tray 9 are rotatably fixed by the positioning ring 18 of lower section On support column 2, positioning ring is fixed on the support columns by clamping screw 19;Anti-skidding rubber is equipped with above the positioning ring Piece;Cabinet body 1 is equipped with opening 10 for accessing article from reagent storage rack and ice bag tray, sets at the opening 10 of cabinet body 1 There is the cabinet door 11 being slidably connected with cabinet body 1, cabinet door is arranged between the upper and lower slide rail on the outside of cabinet body, and cabinet door is surrounded by sealing Item, cabinet door outside are equipped with handle.
The fixed frame 4 is preferably circular, multiple purifying pipe fixing grooves 12 is arranged above, and multiple fixing grooves 12 are around solid It uniformly arranges in a ring in the center of circle for determining frame;Circular fixed frame 4 can be directly placed into matched centrifuge with purifying pipe together and hang It is centrifuged in basket.
Ice bag is arranged in the half of disk upper bottom portion of the ice bag tray 9, and the ice bag rack 15 of multiple annulars is arranged, There is interval between two neighboring annular rack 15 and is used for intercalating agent bottle body.
The different reagent bottle of multiple groups diameter fixed cave 13 is set in the reagent storage rack 8, and every group of fixed cave is opposite to be supported Column is uniformly arranged in a ring, and heat shield 14 is arranged (in order to more clear between two neighboring fixed cave in the fixation cave of same group of annular The positional relationship between cave and ice bag rack is fixed in the signal of Chu, and clipped components in figure are only schematically drawn part Heat shield), reagent bottle is put into when fixing in fixed cave 13, and reagent bottle can be made by rotating reagent storage rack 8 or ice bag tray 9 Body is extend between the ice bag of ice bag tray 9.
The dual function of reagent storage and purification process can be achieved in the DNA purification work platform of the present embodiment;And it can realize pair The effect that the portion of reagent of storage is refrigerated;Sample can be processed in batches and carry out DNA purification, and centrifugal treating can be carried out in batches Improve working efficiency;Different sample room cross contaminations are prevented using cover;With rotatable operating platform, reagent storage rack and Ice bag tray, and height is adjustable, facilitates and picks and places reagent and replacement ice bag, adapts to different personnel's operation and different height Reagent bottle storage.
Embodiment 2
As shown in figure 4, comparing embodiment 1, limited block is also set up on the cover of the present embodiment, limited block is for connecting and fixing Magnetic frame 16;The jack position of the magnetic frame is oppositely arranged with the purifying pipe position in fixed frame 4.The operating platform, examination Agent storage rack, ice bag tray, circular cover body and cylindrical cabinet body are made of transparent plastic material.Using transparent The more convenient observation inner case of material, facilitate experimental implementation.It is other same as Example 1.
Compared with Example 1, the DNA purification work platform of the present embodiment is more convenient extracts DNA using paramagnetic particle method, and use is transparent The more convenient observation inner case of material, facilitate experimental implementation.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.

Claims (9)

1. a kind of DNA purification work platform, which is characterized in that including cylindrical cabinet body, circular support column, cabinet is arranged among cabinet body Round operating platform is arranged in body top, and for setting multiple groups flexible clamp piece for fixed purifying tube fixing device, operation is flat on operating platform Circular cover body is set above platform, and cover side opens up action pane;Reagent storage rack, reagent storage rack are set below operating platform Ice bag tray is arranged in lower section;Operating platform, reagent storage rack and ice bag tray are removably secured on support column, rotation Reagent after turning in reagent storage rack can be contacted with ice bag in ice bag tray;Cabinet body is equipped with opening for from reagent storage rack With access article in ice bag tray, the opening of cabinet body is equipped with the cabinet door being slidably connected with cabinet body.
2. a kind of DNA purification work platform according to claim 1, which is characterized in that the cover opens up action pane Two sides are equipped with the baffle slided up and down.
3. a kind of DNA purification work platform according to claim 1, which is characterized in that the fixed frame be it is circular, above Multiple purifying pipe fixing grooves are set, and multiple fixing grooves are uniformly arranged in a ring around the center of circle of fixed frame;Circular fixed frame can It is centrifuged with being directly placed into matched centrifugal basket.
4. a kind of DNA purification work platform according to claim 1, which is characterized in that be arranged in the reagent storage rack more The fixed cave of the different reagent bottle of group diameter, every group of fixed cave are uniformly arranged in a ring with respect to support column, the fixation of same group of annular Heat shield is set between two neighboring fixed cave in cave, and reagent bottle is put into when fixing in fixed cave, and rotation reagent storage rack can make The body of reagent bottle is extend between the ice bag of ice bag tray.
5. a kind of DNA purification work platform according to claim 4, which is characterized in that one side of something circle of the ice bag tray Ice bag is arranged in disk upper bottom portion, and the ice bag rack of multiple annulars is arranged, and there is interval between two neighboring annular rack and uses In intercalating agent bottle body.
6. according to claim 1 to a kind of 5 described in any item DNA purification work platforms, which is characterized in that also set on the cover Set the limited block for fixing magnetic frame;Purifying pipe position in the jack position and fixed frame of the magnetic frame is oppositely arranged.
7. according to claim 1 to a kind of 5 described in any item DNA purification work platforms, which is characterized in that the operating platform, Reagent storage rack, ice bag tray, circular cover body and cylindrical cabinet body are made of transparent plastic material.
8. according to claim 1 to a kind of 5 described in any item DNA purification work platforms, which is characterized in that the operating platform, Reagent storage rack and ice bag tray are fixed on the support columns by the positioning ring of lower section, and positioning ring is fixed on by clamping screw On support column.
9. a kind of DNA purification work platform according to claim 8, which is characterized in that be equipped with above the positioning ring anti-skidding Sheet rubber.
CN201821810404.7U 2018-11-05 2018-11-05 A kind of DNA purification work platform Active CN209362538U (en)

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CN201821810404.7U CN209362538U (en) 2018-11-05 2018-11-05 A kind of DNA purification work platform

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CN201821810404.7U CN209362538U (en) 2018-11-05 2018-11-05 A kind of DNA purification work platform

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112718038A (en) * 2020-12-18 2021-04-30 无锡市第五人民医院 Clinical pharmacy safety experiment table

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112718038A (en) * 2020-12-18 2021-04-30 无锡市第五人民医院 Clinical pharmacy safety experiment table

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