CN115639368A - Rapid detection kit for respiratory viruses and preparation method thereof - Google Patents
Rapid detection kit for respiratory viruses and preparation method thereof Download PDFInfo
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- CN115639368A CN115639368A CN202211308599.6A CN202211308599A CN115639368A CN 115639368 A CN115639368 A CN 115639368A CN 202211308599 A CN202211308599 A CN 202211308599A CN 115639368 A CN115639368 A CN 115639368A
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- 241000700605 Viruses Species 0.000 title claims abstract description 41
- 230000000241 respiratory effect Effects 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 27
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- 102000036639 antigens Human genes 0.000 claims description 17
- 108091007433 antigens Proteins 0.000 claims description 17
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- 229920001220 nitrocellulos Polymers 0.000 claims description 6
- 241000309551 Arthraxon hispidus Species 0.000 claims description 4
- 238000009434 installation Methods 0.000 abstract description 4
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- 241000725643 Respiratory syncytial virus Species 0.000 description 8
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- 208000015181 infectious disease Diseases 0.000 description 3
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- 208000017667 Chronic Disease Diseases 0.000 description 1
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- 230000001154 acute effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
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- 238000003759 clinical diagnosis Methods 0.000 description 1
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Abstract
The invention relates to the technical field of biology, in particular to a rapid detection kit for respiratory viruses and a preparation method thereof, and the rapid detection kit comprises a box body, a mixing component, a fixing component and an auxiliary component, wherein the mixing component comprises a motor, a fixing column and a rotating frame, the box body provides installation conditions for the mixing component and the fixing component, the mixing component rotates at a high speed to uniformly mix a collected sample and an identification reagent in a test tube, the fixing column is arranged to facilitate the installation of the rotating frame and the output end of the motor, specifically, the motor is started, the electrified output end of the motor drives the fixing column to rotate, and the rotating frame is driven to rotate at a high speed in the box body by the rotation of the fixing column, so that the collected sample and the identification reagent in the test tube are rapidly mixed without manual oscillation, the detection efficiency of the collected sample is improved, the working strength of medical staff is reduced, and the problem that the manual shaking of the uniform sample and reagent are adopted in the detection of the virus of the existing detection kit is solved, and the working strength of the detectors is increased.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a rapid detection kit for respiratory viruses and a preparation method thereof.
Background
Acute respiratory tract infection is the most common infectious disease, has high morbidity and mortality in the world, has the characteristics of strong infectivity, quick transmission, acute morbidity and the like of respiratory tract pathogens, can cause serious complications in children, old people, patients with chronic diseases, malnutrition, low immunity and the like, so that early and accurate judgment of the infection type of the disease is the basis of reasonable application of antibiotic treatment, shortening of the course of the disease and improvement of prognosis, and provides a pathogenic reference basis for clinical diagnosis and treatment, reasonable medication and epidemic disease monitoring.
The existing method for judging the disease infection type adopts a kit for detection, and after a detection sample is collected, the detection sample and an identification reagent need to be vibrated and uniformly mixed, but the existing detection kit does not have a vibrating function and also needs manual vibration, so that the working strength of a detector is increased, and the detection is inconvenient.
Disclosure of Invention
The invention aims to provide a rapid detection kit for respiratory viruses and a preparation method thereof, and aims to solve the problem that the work intensity of a detector is increased by manually shaking up a sample and a reagent in the conventional detection kit for detecting the viruses.
In order to achieve the above object, in a first aspect, the present invention provides a rapid detection kit for respiratory viruses, comprising a box body, a mixing component and a fixing component, wherein the mixing component is arranged in the box body, the fixing component is arranged on the top of the mixing component, and the mixing component comprises a motor, a fixing column and a rotating frame;
the motor with box body fixed connection, and be located in the box body, the fixed column with motor output fixed connection, and be located and keep away from box body one side, the rotating turret with the fixed column is dismantled and is connected, and is located the fixed column outside.
The rotating frame comprises a connector, a sliding frame and an adjusting clamp, the connector is detachably connected with the fixed column and is located on the outer side of the fixed column, the sliding frame is fixedly connected with the connector and is located far away from one side of the fixed column, and the adjusting clamp is arranged in the sliding frame.
The auxiliary assembly comprises a base, a pressing block and a sucking disc, the base is fixedly connected with the box body and located at the bottom of the box body, the pressing block is connected with the base in a sliding mode and located in the base, and the sucking disc is fixedly connected with the pressing block and located in the sucking disc.
Wherein, the balladeur train includes mounting bracket and universal wheel, the mounting bracket with connector fixed connection just is located and keeps away from fixed column one side, the universal wheel with the mounting bracket rotates to be connected, and is located and is close to motor one side.
Wherein, fixed subassembly includes apron, joint and splint, the apron with the balladeur train rotates to be connected, and is located and keeps away from motor one side, the joint with balladeur train fixed connection just is located and keeps away from motor one side, splint with connector sliding connection, and run through the connector.
Wherein, splint include clamping part, second spring and slide, the slide with connector sliding connection, and run through the joint head, the clamping part with slide fixed connection, and be located and be close to apron one side, the second spring with clamping part fixed connection, and with joint head fixed connection, and be located the clamping part with between the connector, the slide has the kerve, the kerve is located the slide is kept away from clamping part one side.
In a second aspect, the present invention also provides a method for preparing a rapid detection kit for respiratory viruses, comprising the following steps:
collecting a virus sample to prepare a virus antigen;
placing the virus antigen and the reagent into a test tube, and uniformly shaking the virus antigen and the reagent in a reagent box to obtain a mixed solution;
and fixing the mixed solution to a nitrocellulose membrane for colored reaction detection to obtain a detection result.
According to the rapid detection kit for the respiratory viruses, the box body provides installation conditions for the mixing assembly and the fixing assembly, the mixing assembly rotates at a high speed to uniformly mix a collected sample and an identification reagent in a test tube, the fixing assembly is used for fixing the test tube to prevent the test tube from loosening and damaging when the mixing assembly rotates, the fixing column is arranged to facilitate the connection of the rotating frame and the output end of the motor, specifically, a medical worker starts the motor by using a hand, the electrified output end of the motor drives the fixing column to rotate, and the rotating frame is driven by the rotation of the fixing column to rotate at a high speed in the box body, so that the collected sample and the identification reagent in the test tube are rapidly mixed without manual oscillation, the efficiency of the kit for detecting the collected sample is improved, the working strength of the medical worker is reduced, and the problem that the manual shaking-up of the sample and the reagent are adopted in the detection of the viruses of the existing detection kit is solved, and the working strength of the detector is increased.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural diagram of a rapid detection kit for respiratory viruses provided by the present invention.
FIG. 2 is a top view of the rapid detection kit for respiratory viruses provided by the present invention.
Fig. 3 isbase:Sub>A cross-sectional view taken along planebase:Sub>A-base:Sub>A of fig. 2.
FIG. 4 is a top view of the kit for rapid detection of respiratory viruses according to the present invention.
FIG. 5 is a schematic connection diagram of the fixing component of the rapid detection kit for respiratory viruses provided by the present invention.
1-box body, 2-mixing component, 3-fixing component, 4-auxiliary component, 5-motor, 6-fixing column, 7-rotating frame, 8-connecting head, 9-sliding frame, 10-adjusting clamp, 11-mounting frame, 12-universal wheel, 13-cover plate, 14-clamping joint, 15-clamping plate, 16-clamping part, 17-second spring, 18-sliding plate, 19-pull groove, 20-clamping plate, 21-first spring, 22-sliding rod, 23-sleeve, 24-base, 25-pressing block and 26-sucking disc.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
Referring to fig. 1 to 5, the present invention provides a rapid detection kit for respiratory viruses, and in a first aspect, the present invention provides a rapid detection kit for respiratory viruses and a preparation method thereof, including a box body 1, a mixing component 2, a fixing component 3 and an auxiliary component 4, wherein the mixing component 2 is disposed in the box body 1, the fixing component 3 is disposed at the top of the mixing component 2, the auxiliary component 4 is disposed at the outer side of the box body 1, and the mixing component 2 includes a motor 5, a fixing column 6 and a rotating frame 7;
the motor 5 with 1 fixed connection of box body, and be located in the box body 1, fixed column 6 with 5 output end fixed connection of motor, and be located and keep away from 1 one side of box body, rotating turret 7 with 6 dismantlements of fixed column are connected, and are located the 6 outsides of fixed column.
In this embodiment, box body 1 is right mixing subassembly 2 with fixed subassembly 3 provides the installation condition, mixing subassembly 2 rotates at a high speed makes collection sample and the identification reagent homogeneous mixing in the test tube, fixed subassembly 3 is used for fixed test tube, prevents that the not hard up emergence of test tube damages when mixing subassembly 2 rotates, the setting of fixed column 6 is convenient for rotating turret 7 with the connection of motor 5 output, specifically, medical personnel utilize hand starter motor 5, motor 5 circular telegram output drive fixed column 6 rotates, fixed column 6 rotates and drives rotating turret 7 is in high-speed rotation in the box body 1 to make collection sample and the identification reagent rapid mixing in the test tube, need not artifical the vibration, improved the efficiency that the kit detected the collection sample, alleviateed medical personnel's working strength simultaneously, solved current detection kit detection virus and adopted artifical sample and reagent of shaking, increased surveyor person's working strength's problem.
Further, the rotating frame 7 comprises a connector 8, a sliding frame 9 and an adjusting clamp 10, the connector 8 is detachably connected with the fixed column 6 and is located on the outer side of the fixed column 6, the sliding frame 9 is fixedly connected with the connector 8 and is located on one side far away from the fixed column 6, and the adjusting clamp 10 is arranged in the sliding frame 9; balladeur train 9 includes mounting bracket 11 and universal wheel 12, mounting bracket 11 with 8 fixed connection of connector, and be located and keep away from fixed column 6 one side, universal wheel 12 with mounting bracket 11 rotates to be connected, and is located and is close to motor 5 one side.
In this embodiment, through connector 8 will balladeur train 9 mounting bracket 11 is connected on fixed column 6, insert the test tube again in mounting bracket 11's the standing groove, the test tube that gets into the standing groove passes through it is fixed to adjust the centre gripping of clamp 10, the setting of adjusting clamp 10 makes mounting bracket 11 is applicable in the not test tube of equidimension, improves mounting bracket 11's practicality, last motor 5 drive when fixed column 6 rotates, fixed column 6 drives mounting bracket 11 rotates, mounting bracket 11 passes through this moment universal wheel 12 is in slide in the box body 1, realize the pivoted process.
Further, the fixing assembly 3 includes a cover plate 13, a clamping head 14 and a clamping plate 15, the cover plate 13 is rotatably connected with the carriage 9 and located at a side far from the motor 5, the clamping head 14 is fixedly connected with the carriage 9 and located at a side far from the motor 5, and the clamping plate 15 is slidably connected with the clamping head 14 and penetrates through the clamping head 14; splint 15 includes clamping part 16, second spring 17 and slide 18, slide 18 with joint 14 sliding connection, and run through joint 14, clamping part 16 with slide 18 fixed connection, and be located and be close to apron 13 one side, second spring 17 with clamping part 16 fixed connection, and with connector 8 fixed connection, and be located clamping part 16 with between the connector 8, slide 18 has kerve 19, kerve 19 is located slide 18 is kept away from clamping part 16 one side.
In this embodiment, the clamping head 14 provides a mounting condition for the clamping plate 15, the cover plate 13 is inserted into the clamping head 14 and fixed to the clamping plate 15, so as to fix the top of the test tube in the mounting frame 11, and prevent the mounting frame 11 from rotating, so that the test tube rotating at a high speed is loosened and damaged, specifically, after the test tube is placed in the placement groove of the mounting frame 11, a medical staff pulls the sliding plate 18 with a hand to slide the sliding plate 18 to a side away from the cover plate 13, thereby driving the clamping portion 16 to slide in the clamping head 14, at this time, the clamping portion 16 compresses the second spring 17, and again rotates the cover plate 13 with a hand to press the top of the water tube by the cover plate 13 and insert the top of the water tube into the clamping head 14, and releases the sliding plate 18, the second spring 17 resets to push the sliding plate 18 and the clamping portion 16 to reset, the clamping portion 16 firmly clamps the cover plate 13 in the clamping head 14, that is fixed, and the guide rod is used for guiding the adjustment clamp 10 to prevent the adjustment clamp 10 from shifting and affecting the clamping effect of the test tube, and the clamping plate 19 is set for the medical staff to pull the groove 18.
Further, the auxiliary assembly 4 includes a base 24, a pressing block 25 and a suction cup 26, the base 24 is fixedly connected with the box body 1 and is located at the bottom of the box body 1, the pressing block 25 is slidably connected with the base 24 and is located in the base 24, and the suction cup 26 is fixedly connected with the pressing block 25 and is located in the suction cup 26.
In this embodiment, the base 24 provides a mounting condition for the pressing block 25, and the pressing block 25 is pressed to press the pressing block 25 into the base 24, so as to push the suction cup 26 to press down to contact with a desktop, wherein the suction cup 26 is used for adsorbing the desktop to fix the box body 1, and the box body 1 is prevented from moving on the desktop when a detection sample and an identification reagent are mixed.
Further, the adjusting clip 10 includes a clip piece 20 and a first spring 21, the first spring 21 is fixedly connected to the sliding frame 9 and located in the sliding frame 9, the clip piece 20 is fixedly connected to the first spring 21 and located on one side of the first spring 21.
In this embodiment, the clamping piece 20 clamps and fixes the inserted test tube by the reaction of the first spring 21, and simultaneously, the position of the clamping piece 20 in the placing hole of the mounting rack 11 is adjusted by the arrangement of the first spring 21, so that the mounting rack 11 can be applied to test tubes of different sizes.
Further, the guide bar includes a sliding rod 22 and a sleeve 23, the sleeve 23 is fixedly connected to the sliding frame 9 and located inside the sliding frame 9, the sliding rod 22 is slidably connected to the sleeve 23 and fixedly connected to the clamping piece 20 and located between the sleeve 23 and the clamping piece 20.
In this embodiment, when the first spring 21 deforms and stretches out and draws back, the sliding rod 22 slides in the sleeve 23, it is right to guide the clamping piece 20, and it is right to prevent that the influence is right after the first spring 21 laterally deflects the clamping effect of the clamping piece 20 to the test tube.
In a second aspect, the present invention also provides a method for preparing a rapid detection kit for respiratory viruses, comprising the following steps:
s1, collecting a virus sample to prepare a virus antigen;
artificially synthesizing an optimized respiratory syncytial virus F protein antigen partial gene sequence F1, constructing a prokaryotic expression vector, expressing respiratory syncytial virus F1 antigen protein by using escherichia coli, and renaturing the inclusion body by adopting a dialysis method, a gradient dilution method and a gel chromatography method to obtain the recombinant respiratory syncytial virus F1 protein antigen with a three-dimensional structure and immunocompetence.
S2, placing the virus antigen and the reagent into a test tube, and uniformly shaking the virus antigen and the reagent in a reagent box to obtain a mixed solution;
after the test tube was put into mounting bracket 11's standing groove, medical personnel utilized the hand pulling slide 18, made slide 18 to keeping away from apron 13 one side slides, thereby drives clamping part 16 is in slide in the connector 8, at this moment clamping part 16 compresses the spring, and reuse hand rotates apron 13 makes apron 13 pushes down the water pipe top and inserts in the connector 8, unclamp slide 18, the spring resets and promotes slide 18 with clamping part 16 resets, clamping part 16 then will apron 13 firmly presss from both sides in the connector 8, accomplish promptly to the fixing of test tube, starter motor 5, the drive of motor 5 circular telegram output end fixed column 6 rotates, fixed column 6 rotates and drives rotating turret 7 is in high-speed rotation in the box body 1 to make the collection sample and the fast mixing of appraisal reagent in the test tube, obtain mixed liquid.
S3, fixing the mixed solution to a nitrocellulose membrane for colored reaction detection to obtain a detection result.
Fixing the mixed solution on a nitrocellulose membrane, fixing a labeled antibody (secondary antibody) on a solid phase carrier, contacting a sample to be detected with the labeled antibody (secondary antibody), forming an antibody-labeled secondary antibody complex by the respiratory syncytial virus antibody and the labeled secondary antibody if the respiratory syncytial virus antibody exists in the sample to be detected, enabling the complex to horizontally move on the nitrocellulose membrane, and when the complex meets the respiratory syncytial virus antigen fixed on the membrane to generate reaction, the complex with the label is specifically fixed on the antigen, and the position of the fixed antigen has a color reaction; if the sample to be detected does not contain the respiratory syncytial virus antibody, the complex can not be fixed on the position of the antigen, no color reaction exists here, and the detection result, namely whether the respiratory syncytial virus antibody exists in the sample to be detected can be judged according to whether the position of the fixed antigen of the nitrocellulose membrane has the color reaction.
The invention also provides a preparation method of the rapid detection kit for the respiratory viruses, which comprises the following steps:
although the above-described preferred embodiments of the rapid test kit for respiratory viruses and the preparation method thereof are disclosed in the present invention, it is understood that the scope of the present invention is not limited thereto, and those skilled in the art can understand that all or part of the procedures for implementing the above-described embodiments and equivalent variations made according to the claims of the present invention still fall within the scope of the present invention.
Claims (7)
1. A rapid detection kit for respiratory viruses is characterized in that,
the mixing device comprises a box body, a mixing component, a fixing component and an auxiliary component, wherein the mixing component is arranged in the box body, the fixing component is arranged at the top of the mixing component, the auxiliary component is arranged on the outer side of the box body, and the mixing component comprises a motor, a fixing column and a rotating frame;
the motor with box body fixed connection, and be located in the box body, the fixed column with motor output fixed connection, and be located and keep away from box body one side, the rotating turret with the fixed column is dismantled and is connected, and is located the fixed column outside.
2. The rapid detection kit for a respiratory virus according to claim 1,
the rotating turret includes connector, balladeur train and regulation clamp, the connector with the fixed column is dismantled and is connected, and is located the fixed column outside, the balladeur train with connector fixed connection just is located and keeps away from fixed column one side, the regulation clamp is established in the balladeur train.
3. The rapid detection kit for a respiratory virus according to claim 2,
the auxiliary assembly comprises a base, a pressing block and a sucker, the base is fixedly connected with the box body and located at the bottom of the box body, the pressing block is slidably connected with the base and located in the base, and the sucker is fixedly connected with the pressing block and located in the sucker.
4. The rapid detection kit for a respiratory virus according to claim 2,
the balladeur train includes mounting bracket and universal wheel, the mounting bracket with connector fixed connection just is located and keeps away from fixed column one side, the universal wheel with the mounting bracket rotates to be connected, and is located and is close to motor one side.
5. The rapid detection kit for a respiratory virus according to claim 2,
the fixed subassembly includes apron, joint and splint, the apron with the balladeur train rotates to be connected, and is located and keeps away from motor one side, the joint with balladeur train fixed connection just is located and keeps away from motor one side, splint with connector sliding connection, and run through the connector.
6. The rapid detection kit for a respiratory virus according to claim 5,
splint include clamping part, second spring and slide, the slide with connector sliding connection, and run through the joint head, the clamping part with slide fixed connection, and be located and be close to apron one side, the second spring with clamping part fixed connection, and with joint head fixed connection, and be located the clamping part with between the connector, the slide has the kerve, the kerve is located the slide is kept away from clamping part one side.
7. The method for preparing a rapid detection kit for respiratory viruses, which is applied to the rapid detection kit for respiratory viruses according to claim 1, is characterized by comprising the following steps:
collecting a virus sample to prepare a virus antigen;
placing the virus antigen and the reagent into a test tube, and uniformly shaking the virus antigen and the reagent in a reagent box to obtain a mixed solution;
and fixing the mixed solution to a nitrocellulose membrane for colored reaction detection to obtain a detection result.
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| CN202211308599.6A CN115639368B (en) | 2022-10-25 | 2022-10-25 | Rapid detection kit for respiratory viruses and preparation method thereof |
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| CN117699211A (en) * | 2023-12-27 | 2024-03-15 | 梁洪彬 | Kit for detecting department of anesthesia for collecting saliva samples |
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| CN114432954A (en) * | 2022-01-25 | 2022-05-06 | 王水琴 | Even equipment of shaking is used in detection of hematology branch of academic or vocational study vein blood sample |
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| CN117699211A (en) * | 2023-12-27 | 2024-03-15 | 梁洪彬 | Kit for detecting department of anesthesia for collecting saliva samples |
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