CN210620836U

CN210620836U - Novel cell culture bag with acid-base indicator and culture medium separated

Info

Publication number: CN210620836U
Application number: CN201920487798.5U
Authority: CN
Inventors: 王媛媛; 刘进平; 李建伟; 魏永; 董宁宁; 王睿升
Original assignee: Shanxi Junnuo Kangyuan Biotechnology Co Ltd
Current assignee: Shanxi Junnuo Kangyuan Biotechnology Co Ltd
Priority date: 2019-04-11
Filing date: 2019-04-11
Publication date: 2020-05-26
Anticipated expiration: 2029-04-11

Abstract

The utility model discloses a novel cell culture bag of acid-base indicator and culture medium separation belongs to biological cell culture field. The cell culture bag is mainly composed of a main cell culture bag and an auxiliary cell culture bag, wherein the main cell culture bag is connected with the auxiliary cell culture bag through a guide pipe, and a device for controlling the on-off of the guide pipe is arranged on the guide pipe. The volume of the auxiliary cell culture bag is smaller than that of the main cell culture bag, and the auxiliary cell culture bag is used for storing a small amount of cell culture medium and an acid-base indicator which are obtained from the main cell culture bag. The method solves the general defects in the prior art that the pH value of the culture medium needs to be indicated by phenol red in the culture medium during cell culture, but the phenol red component in the culture medium has undesirable influence on the cell culture, and the pH value range of the culture medium cannot be judged by using the culture medium without adding the phenol red. The method has the advantages that phenol red can be used for indicating the pH value of the cell culture medium without influencing cell culture.

Description

Novel cell culture bag with acid-base indicator and culture medium separated

Technical Field

The utility model belongs to biological cell culture field, concretely relates to cell culture bag.

Background

The culture medium in cell culture should satisfy the requirements of cells on nutrient components, growth promoting factors, hormones, osmotic pressure and the like, and also satisfy the requirement of pH value. Most animal cells require slightly alkaline conditions, and the pH value is about 7.2-7.4. However, this pH is affected by the gases required for cell survival, which are among the essential conditions for cell survival, mainly oxygen and carbon dioxide. Oxygen is involved in the tricarboxylic acid cycle, providing the cell with the ability to grow, proliferate and synthesize various components. Carbon dioxide is a cell metabolite and a component required by cells, and the carbon dioxide directly influences the pH value of a cell culture medium, because the carbon dioxide is continuously released along with the increase of the number of the cells and the enhancement of metabolic activity in the cell growth process, and the increase of the carbon dioxide in the cell culture medium gradually becomes acidic, namely the pH value is reduced, so that the real-time observation of the change of the pH value of the cell culture medium is very important for grasping the influence of the pH value on the cell culture process or adopting necessary measures to control the pH value.

Currently, a culture medium added with phenol red is mostly used for culturing cells in a laboratory, and the pH value of the culture medium is indicated by the phenol red: the color is red in neutral, yellow in acidic and purple in alkaline. The range of the pH value of the medium can be judged by visual observation. However, the phenol red component of the medium may mimic the effects of human steroid hormones, particularly estrogens. Therefore, in order to avoid the steroid reaction, a medium to which phenol red is not added is generally used for culturing mammalian cells, but the pH range of the culture solution cannot be determined when a phenol red-free medium is used.

Therefore, it is necessary to develop a technology that can indicate the pH of a cell culture medium using phenol red without affecting cell culture.

Disclosure of Invention

In order to solve the problem, the utility model provides a can instruct culture medium pH value and not influence cell culture's novel cell culture bag again, realized the separation of cell culture medium and phenol red indicator and can be real-time, survey the pH value of culture medium in succession.

The utility model discloses a following technical scheme realizes: the novel cell culture bag comprises a main cell culture bag and an auxiliary cell culture bag, wherein the main cell culture bag and the auxiliary cell culture bag are connected through a guide pipe, and a device for controlling the connection and disconnection of the guide pipe is arranged on the guide pipe.

The volume of the auxiliary cell culture bag is smaller than that of the main cell culture bag, and the auxiliary cell culture bag is used for storing a small amount of cell culture medium and an acid-base indicator which are obtained from the main cell culture bag.

During the use, the pipe through main cell culture bag edge and external intercommunication injects the culture medium into the main cell culture bag and is used for culturing the cell, then control on the pipe of connecting main cell culture bag and supplementary cell culture bag control to lead the pipe to make, disconnected device makes the pipe be in the connected state, make appropriate amount cell culture medium flow into supplementary cell culture bag through the pipe, control on the pipe is followed and is led the pipe to make, disconnected device makes this pipe be in the non-connected state, follow that to inject appropriate amount concentration appropriate phenol red reagent into supplementary cell culture bag through the pipe of supplementary cell culture bag edge and external intercommunication, seal each mouth of pipe of main cell culture bag and supplementary cell culture bag at last, and put into it and carry out cell culture in the cell culture equipment. And the pH value is judged by observing the color of a culture medium system in the auxiliary cell culture bag at any time in the culture process.

The utility model has the advantages as follows:

(1) the indicator is separated from the cell culture medium, so that the influence of the indicator on the cell culture medium is avoided, and particularly, the sterol reaction is avoided. Placing the cell culture medium in a main cell culture bag, taking a small amount of the cell culture medium to enter an auxiliary cell culture bag, and injecting a proper amount of phenol red indicator into the auxiliary cell culture bag, so that the culture medium in the main cell culture bag is separated from the phenol red indicator in the auxiliary cell culture bag, and the influence of the phenol red indicator on the culture medium in the main cell culture bag is avoided;

(2) the acid-base condition in the auxiliary cell culture bag can accurately reflect the acid-base condition in the main cell culture bag. The cell culture medium in the auxiliary cell culture bag is taken from the main cell culture bag, has the same components and state as the culture medium in the main cell culture bag, is equivalent to a sample of the culture medium in the main cell culture bag, can completely represent the culture medium in the main cell culture bag in composition and property, is simultaneously placed in cell culture equipment, is completely in the same cell culture environment, can completely represent the culture condition of cells in the main cell culture bag in the auxiliary cell culture bag, and completely represents the acidity and alkalinity of the main cell culture bag in the acid-base performance indicated by phenol red in the auxiliary cell culture bag;

(3) frequent sampling is not required and the observation of the acid-base condition is real-time, continuous. At present, in order to avoid phenol red reagent to produce the influence and not use phenol red reagent to cell culture, need follow the sample in the cell culture bag and indicate its acid-base nature in cell culture bag external phenol red reagent when wanting to know the acid-base nature of cell culture medium, the drawback of this kind of method lies in troublesome poeration, and observes not continuously, and the observation result is influenced by the frequency of sample observation to the reaction of true condition, the technical scheme of the utility model, realized not using phenol red reagent in the main cell culture bag, but can indirectly know the acid-base nature condition in the main cell culture bag through assisting cell culture bag, do not need frequent sample, and the observation of acid-base nature condition is real-time, continuous.

Drawings

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

Fig. 1 is a schematic structural diagram of the present invention.

In the figure: 1-main cell culture bag, 2-auxiliary cell culture bag, 3-clamp and 4-conduit.

Detailed Description

The novel cell culture bag with the acid-base indicator separated from the culture medium comprises a main cell culture bag and an auxiliary cell culture bag, wherein the main cell culture bag is connected with the auxiliary cell culture bag through a guide pipe, a clamp for controlling the on-off of the guide pipe is arranged on the guide pipe, and the guide pipe is made of a soft material. The volume of the auxiliary cell culture bag is smaller than that of the main cell culture bag, a small amount of cell culture medium and an acid-base indicator are stored and obtained from the main cell culture bag, two guide pipes communicated with the outside are arranged at the edge of the main cell culture bag, and two guide pipes communicated with the outside are also arranged at the edge of the auxiliary cell culture bag.

During the use, the pipe through main cell culture bag edge and external intercommunication injects the culture medium into in the main cell culture bag and is used for culturing the cell, then open the clip on the pipe of connecting main cell culture bag and supplementary cell culture bag, make proper amount cell culture medium flow into supplementary cell culture bag through the pipe, loosen the clip afterwards and make this pipe be in the non-connected state by the clip clamp, after that inject proper amount concentration appropriate phenol red reagent into in the supplementary cell culture bag through the pipe of supplementary cell culture bag edge and external intercommunication, seal each mouth of pipe of main cell culture bag and supplementary cell culture bag at last, and put into it and carry out cell culture in the cell culture equipment. And the pH value is judged by observing the color of a culture medium system in the auxiliary cell culture bag at any time in the culture process.

Claims

1. The novel cell culture bag is characterized by comprising a main cell culture bag and an auxiliary cell culture bag, wherein the main cell culture bag and the auxiliary cell culture bag are connected through a guide pipe, and a device for controlling the on-off of the guide pipe is arranged on the guide pipe.

2. The novel cell culture bag with acid-base indicator separated from culture medium of claim 1, wherein the volume of the auxiliary cell culture bag is smaller than that of the main cell culture bag, and the auxiliary cell culture bag is used for storing a small amount of cell culture medium and acid-base indicator obtained from the main cell culture bag.