CN210470813U

CN210470813U - Amnion tissue cryopreservation tank

Info

Publication number: CN210470813U
Application number: CN201921146669.6U
Authority: CN
Inventors: 任贺; 邢淑荣
Original assignee: Medsooner Medical Technology Tianjin Co ltd
Current assignee: Medsooner Medical Technology Tianjin Co ltd
Priority date: 2019-07-19
Filing date: 2019-07-19
Publication date: 2020-05-08
Anticipated expiration: 2029-07-19

Abstract

The utility model relates to an express delivery letter sorting technical field discloses an amnion tissue cryopreserving jar, include: the refrigerator comprises a conversion mechanism, a box body mechanism, a front door mechanism, a display screen and a freezing and storing mechanism, wherein the conversion mechanism comprises an inlet pipeline, the inlet pipeline is installed and fixed on the upper portion of the box body mechanism, the box body mechanism comprises a box body shell, the front portion of the box body shell is fixedly provided with the front door mechanism, the front portion of the box body shell is provided with the display screen, the display screen is installed on the lower portion of the front door mechanism, and the freezing and storing mechanism is fixedly installed inside the box body mechanism. The conversion mechanism further comprises a conversion head, a dry ice inlet and an ice block inlet, the dry ice inlet and the ice block inlet are fixedly arranged on the upper portion of the inlet pipeline, and the conversion head is fixedly arranged on the upper portions of the dry ice inlet and the ice block inlet. The utility model discloses a novel gel form frozen stock solution is frozen the final amnion tissue and is deposited, and the amnion after freezing the deposit has high activity, prolongs the date of keeping to can be used to clinical use.

Description

Amnion tissue cryopreservation tank

Technical Field

The utility model relates to an amniotic tissue cryopreserved technical field especially relates to an amniotic tissue cryopreserved jar.

Background

At present, most technical modes in the stem cell storage industry are that the amniotic membrane tissue is digested or cultured to obtain mesenchymal stem cells, and the cells are frozen and stored. However, the amnion tissue has more epithelial cells and collagen tissue besides the mesenchymal stem cells, and the tissue cryopreservation method is not mature.

In addition, glycerol or DMSO is used as a basis in the conventional amnion tissue cryopreservation, the tissue activity loss is serious usually in 3-9 months, the novel gel-like cryopreservation liquid is used for performing cryopreservation on the tissue in the research, the cell and protein components in the amnion tissue can be maintained for a long time, and the amnion tissue preserved by the aid of the technology can be directly applied to the clinical field of tissue engineering, so that the application range is expanded.

The novel gel frozen stock solution is matched with a gradient cooling program to freeze the amniotic tissues, so that the frozen amniotic membranes have high activity, cells can be cultured after long-term preservation, the frozen amniotic membranes can be directly used in the tissue engineering field and widely used clinically, and the frozen amniotic tissues have important significance for freezing and clinical use.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, the utility model aims to provide an amnion tissue cryopreservation tank, which adopts novel gel-like cryopreservation liquid to finally cryopreserve amnion tissues, and amnion after cryopreservation has high activity, prolongs the preservation date and can be used for clinical use.

The utility model discloses a following technical scheme realizes: an amniotic membrane tissue cryopreservation canister comprising: the refrigerator comprises a conversion mechanism, a box body mechanism, a front door mechanism, a display screen and a freezing and storing mechanism, wherein the conversion mechanism comprises an inlet pipeline, the inlet pipeline is fixedly arranged on the upper portion of the box body mechanism, the box body mechanism comprises a box body shell, the front door mechanism is fixedly arranged on the front portion of the box body shell, the display screen is arranged on the lower portion of the front door mechanism, and the freezing and storing mechanism is fixedly arranged in the box body mechanism.

Furthermore, the conversion mechanism also comprises a conversion head, a dry ice inlet and an ice block inlet, wherein the dry ice inlet and the ice block inlet are fixedly arranged at the upper part of the inlet pipeline, the conversion heads are fixedly arranged at the upper parts of the dry ice inlet and the ice block inlet, firstly, the ice block is used for cooling in a first gradient, then, the dry ice is injected for cooling in a second gradient, and the conversion of the inlet is completed by the conversion head.

Furthermore, the box mechanism further comprises a box upper cover, a sealing cover, an outlet pipe and a box sealing layer, the box sealing layer is fixedly arranged in the box shell, the sealing cover is fixedly arranged on the upper portion of the box shell, the box upper cover is fixedly arranged on the upper portion of the sealing cover, and the sealing cover can be used for ensuring that the temperature in the box is not influenced by the external environment.

Furthermore, the front door mechanism comprises a front door, an observation window and a front door handle, the front door is fixedly arranged at the front part of the box body shell, the observation window is fixedly arranged on the front door, the front door handle is fixedly arranged on the right side of the front door, the front door is opened to place the amniotic membrane tissues to be cryopreserved into the cryopreservation tank body, and the observation window can observe the cooling process at any time.

Further, the mechanism is deposited to freezing include cooling chamber, export mesh, freeze and deposit jar body filling hole, temperature sensor, frozen stock liquid injection pipeline and external joint, freeze and deposit jar body and install the inside at the box sealing layer, freeze and deposit jar body and box sealing layer between be equipped with the cooling chamber, cooling chamber bottom install the export mesh, freeze and deposit jar body top be equipped with and freeze and deposit jar body filling hole, box sealing layer inboard installation be fixed with temperature sensor, display screen all be connected with the controller through the wire, the controller uses STM32 single chip microcomputer controller commonly used, its low power dissipation, the processing information ability reinforce, gather the inboard temperature transmission of box sealing layer to STM32 single chip microcomputer controller through temperature sensor, show the screen real-time display temperature through STM32 single chip microcomputer controller control, freeze and deposit jar body left side installation and be fixed with frozen stock liquid injection pipeline, the left side of the freezing solution injection pipeline is fixedly provided with an external connector, the external connector is connected with an external freezing solution injection mechanism, and the freezing solution is injected into the amniotic tissues to be preserved.

Further, the outlet pipe is connected with the outlet mesh, and the melted ice blocks and the dry ice can flow out through the outlet pipe.

Compared with the prior art, the beneficial effects of the utility model reside in that:

1. the utility model provides an amnion tissue freezes deposits jar, the utility model discloses a gradient cooling mode, the first order cooling adopts the ice-cube cooling in cooling chamber, and the dry ice cooling is adopted in the second grade cooling, adopts the gel form frozen stock liquid of no DMSO at last to freeze and deposits the tissue, by the gradient cooling, finally realizes high active tissue frozen stock.

2. The utility model provides an amnion tissue freezes deposits jar, the utility model discloses a gel form frozen stock solution that does not have DMSO freezes deposits the tissue, reduces the cytotoxic reaction, at the in-process of gradient cooling, adopts temperature sensor and display screen to survey the change of temperature in real time.

3. The utility model provides an amnion tissue freezes deposits jar, the utility model discloses a conversion head, dry ice import and ice-cube import, when the cooling chamber needs first order or second level cooling, adopt the conversion head to carry out the injection of ice-cube or dry ice, realize the gradient cooling.

Drawings

FIG. 1 is a front view of an amniotic membrane tissue cryopreservation tank of the present invention;

FIG. 2 is a view showing an internal structure of an amnion tissue cryopreservation tank of the present invention;

FIG. 3 is a schematic view of the external structure of an amnion tissue cryopreservation tank of the present invention;

fig. 4 is a schematic structural diagram of a freezing mechanism of the amnion tissue freezing tank of the present invention.

In the figure: 1. a switching mechanism; 2. a box mechanism; 3. a front door mechanism; 4. a display screen; 5. a freezing and storing mechanism; 101. a conversion head; 102. a dry ice inlet; 103. an ice block inlet; 104. an inlet duct; 201. the box body is covered; 202. a sealing cover; 203. a case body shell; 204. an outlet pipe; 205. a box body sealing layer; 301. a front door; 302. an observation window; 303. a front door handle; 501. a cooling cavity; 502. an outlet mesh; 503. freezing and storing the tank body; 504. freezing and storing the tank injection hole; 505. a temperature sensor; 506. the frozen stock solution is injected into a pipeline; 507. an external joint.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that the embodiments or technical features described below can be arbitrarily combined to form a new embodiment without conflict.

As shown in fig. 1 to 4, an amniotic membrane tissue cryopreservation canister comprises: the conversion mechanism 1 comprises an inlet pipeline 104, the inlet pipeline 104 is fixedly arranged on the upper portion of the box body mechanism 2, the box body mechanism 2 comprises a box body shell 203, the front door mechanism 3 is fixedly arranged on the front portion of the box body shell 203, the display screen 4 is arranged on the lower portion of the front door mechanism 3, and the freezing storage mechanism 5 is fixedly arranged in the box body mechanism 2.

Furthermore, the conversion mechanism 1 further comprises a conversion head 101, a dry ice inlet 102 and an ice inlet 103, the dry ice inlet 102 and the ice inlet 103 are fixedly arranged at the upper part of the inlet pipeline 104, the conversion head 101 is fixedly arranged at the upper parts of the dry ice inlet 102 and the ice inlet 103, firstly, the ice is used for cooling in a first gradient, then, the dry ice is injected for cooling in a second gradient, and the conversion of the inlet is completed by means of the conversion head 101.

Further, the box mechanism 2 further comprises a box upper cover 201, a sealing cover 202, an outlet pipe 204 and a box sealing layer 205, the box sealing layer 205 is fixedly installed inside the box shell 203, the box sealing layer 205 is made of aluminum silicate heat-insulating plates, the heat conductivity coefficient of the box sealing layer 205 is low, the box sealing layer has good sealing and heat-insulating performance, the sealing cover 202 is fixedly installed on the upper portion of the box shell 203, the outlet pipe 204 is arranged at the side end of the bottom of the box shell 203, the box upper cover 201 is fixedly installed on the upper portion of the sealing cover 202, and the temperature inside the box can be guaranteed not to be affected by the external environment by using the sealing cover 202.

Further, the front door mechanism 3 comprises a front door 301, an observation window 302 and a front door handle 303, the front door 301 is fixedly installed at the front part of the box body shell 203, the observation window 302 is fixedly installed on the front door 301, the front door handle 303 is fixedly installed at the right side of the front door 301, the front door is opened to place the amniotic tissues to be cryopreserved into the cryopreservation tank body 503, and the observation window 302 can observe the cooling process at any time.

Further, the cryopreservation mechanism 5 comprises a cooling cavity 501, an outlet mesh 502, a cryopreservation tank body 503, a cryopreservation tank body injection hole 504, a temperature sensor 505, a cryopreservation liquid injection pipeline 506 and an external connector 507, wherein the cryopreservation tank body 503 is installed inside a tank body sealing layer 205, the cooling cavity 501 is arranged between the cryopreservation tank body 503 and the tank body sealing layer 205, the outlet mesh 502 is installed at the bottom of the cooling cavity 501, the cryopreservation tank body injection hole 504 is arranged at the top of the cryopreservation tank body 503, the temperature sensor 505 is fixedly installed on the inner side of the tank body sealing layer 205, the specific model of the temperature sensor 505 is pt100, the temperature range can be measured, the maximum allowable temperature deviation is-200 ℃ to 200 ℃, the temperature sensor 505 and the display screen 4 are both connected with a controller through leads, and the controller uses a common STM32 single chip microcomputer controller, the amnion tissue cryopreservation system is low in power consumption and high in information processing capacity, the specific model of the display screen 4 is a 7-inch USART HMI intelligent serial port screen, the display screen is in resistance type touch, the resolution ratio is 800 × 480, the working voltage is 4.75V-7V, the temperature of the inner side of the box body sealing layer 205 is collected through the temperature sensor 505 and transmitted to an STM32 single chip microcomputer controller, the display screen 4 is controlled to display the temperature in real time through the STM32 single chip microcomputer controller, the left side of the cryopreservation tank body 503 is fixedly provided with a cryopreservation liquid injection pipeline 506, the left side of the cryopreservation liquid injection pipeline 506 is fixedly provided with an external connector 507, the external connector 507 is connected with an external cryopreservation liquid injection mechanism, the amnion tissue to be preserved is injected with the cryopreservation liquid, and the cryopreservation liquid is specifically gel-like cryopreservation liquid.

Further, the outlet pipe 204 is connected to the outlet mesh 502, and the melted ice pieces and the dry ice can flow out through the outlet pipe 204.

In a specific embodiment of the design, as shown in fig. 1 to 4, in the tissue cryopreservation process, different cooling materials of a dry ice inlet 102 and an ice inlet 103 are injected into a cooling cavity 501 by means of a conversion head 101, the temperature of the cooling cavity 501 can be observed at any time through a display screen 4, a box upper cover 201, a sealing cover 202, a box outer shell 203 and a box sealing layer 205 are integrally adopted to seal a cryopreservation mechanism 5 so as to isolate temperature exchange with the external environment, a tissue to be cryopreserved is stored in a cryopreservation tank body 503, a cryopreservation liquid injection pipeline 506 and an external connector 507 are installed on the left side of the cryopreservation tank body 503, the cryopreservation liquid is connected with the external cryopreservation liquid injection mechanism by using the external connector 507 so as to inject the amniotic tissue to be reserved, the cryopreservation liquid is specifically gel-like cryopreservation liquid without DMSO, in addition, an outlet mesh 502 is installed at the bottom of the cooling cavity 501, and the outlet mesh 502 is connected with the outlet mesh 502, the melted ice pieces and dry ice can flow out through outlet pipe 204.

It should be understood that although the present description refers to embodiments, not every embodiment contains only a single technical solution, and such description is for clarity only, and those skilled in the art should take the description as a whole, and the technical solutions in the embodiments may be appropriately combined to form other embodiments understood by those skilled in the art.

Claims

1. An amniotic membrane tissue cryopreservation tank, comprising: conversion mechanism (1), box mechanism (2), front door mechanism (3), display screen (4) and freeze and deposit mechanism (5), conversion mechanism (1) including inlet pipe (104), inlet pipe (104) installation fix on the upper portion of box mechanism (2), box mechanism (2) including box shell (203), box shell (203) front portion installation be fixed with front door mechanism (3), box shell (203) front portion still install display screen (4), display screen (4) install the lower part of door mechanism (3) in the front, box mechanism (2) internally mounted be fixed with freeze and deposit mechanism (5).

2. The amniotic membrane tissue cryopreservation pot of claim 1, wherein: the conversion mechanism (1) further comprises a conversion head (101), a dry ice inlet (102) and an ice block inlet (103), the dry ice inlet (102) and the ice block inlet (103) are fixedly arranged on the upper portion of the inlet pipeline (104), and the conversion head (101) is fixedly arranged on the upper portions of the dry ice inlet (102) and the ice block inlet (103).

3. The amniotic membrane tissue cryopreservation pot of claim 1, wherein: the box mechanism (2) further comprises a box upper cover (201), a sealing cover (202), an outlet pipe (204) and a box sealing layer (205), the box sealing layer (205) is fixedly arranged in the box shell (203), the sealing cover (202) is fixedly arranged on the upper portion of the box shell (203), the outlet pipe (204) is arranged at the side end of the bottom of the box shell (203), and the box upper cover (201) is fixedly arranged on the upper portion of the sealing cover (202).

4. The amniotic membrane tissue cryopreservation pot of claim 1, wherein: the front door mechanism (3) comprises a front door (301), an observation window (302) and a front door handle (303), wherein the front door (301) is fixedly arranged at the front part of the box body shell (203), the observation window (302) is fixedly arranged on the front door (301), and the front door handle (303) is fixedly arranged at the right side of the front door (301).

5. The amniotic membrane tissue cryopreservation pot of claim 1, wherein: the freezing mechanism (5) comprises a cooling cavity (501), outlet meshes (502), a freezing tank body (503), a freezing tank body injection hole (504), a temperature sensor (505), a freezing liquid injection pipeline (506) and an external joint (507), wherein the freezing tank body (503) is installed inside a box body sealing layer (205), the cooling cavity (501) is arranged between the freezing tank body (503) and the box body sealing layer (205), the outlet meshes (502) are installed at the bottom of the cooling cavity (501), the freezing tank body injection hole (504) is arranged at the top of the freezing tank body (503), the temperature sensor (505) is installed and fixed on the inner side of the box body sealing layer (205), the temperature sensor (505) and a display screen (4) are connected with a controller through leads, the freezing tank body (503) is installed and fixed with the freezing liquid injection pipeline (506) on the left side, an external joint (507) is fixedly arranged at the left side of the freezing solution injection pipeline (506).

6. The amniotic membrane tissue cryopreservation pot of claim 3, wherein: the outlet pipe (204) is connected with the outlet mesh (502).