CN210328951U - Bee larva breeding device - Google Patents
Bee larva breeding device Download PDFInfo
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- CN210328951U CN210328951U CN201920962766.6U CN201920962766U CN210328951U CN 210328951 U CN210328951 U CN 210328951U CN 201920962766 U CN201920962766 U CN 201920962766U CN 210328951 U CN210328951 U CN 210328951U
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Abstract
The utility model belongs to the technical field of insect breeding devices, in particular to a bee larva breeding device which is provided with an incubator and a climatic chamber, wherein the incubator is positioned in the climatic chamber; the incubator is provided with an incubator bottom surface, an incubator side surface, a cover, N baffles, a cell culture plate and a humidity/temperature probe, wherein the cover is positioned at the top end of the incubator side surface, the incubator is hollow, the baffles are arranged on the incubator bottom surface, the humidity/temperature probe is arranged on the outer surface of the incubator side surface, and the cell culture plate is positioned at the top end of the baffles; the climate box is formed by connecting an upper part, a middle part and a lower part in sequence, wherein the upper part is provided with a display, the lower part is provided with M heat dissipation holes, and the middle part is hollow; one side surface of the middle part is fixedly provided with a door, and the inside of the middle part is provided with a humidified gas input pipe, a bracket, a standby box and a heater. Simple structure, convenient operation, low cost and high survival rate of larvae.
Description
Technical Field
The utility model belongs to the technical field of the insect breeding device, especially, relate to a honeybee larva breeding device.
Background
The bee consists of queen bee, male bee and worker bee, wherein the queen bee and worker bee are developed from fertilized ovum, and the male bee is developed from unfertilized ovum cell. The bee is a completely metamorphic and developmental insect, and the three types of bees pass through 4 developmental stages of eggs, larvae, pupae and imagoes (adult bees). The 4 stages of bees are morphologically different.
The white worm of bee larva is C-shaped initially, and as the larva grows up, the larva straightens and the head faces the honeycomb. In nature, worker bees feed the larvae. The fertilized eggs are hatched into female larvae, and if larva slurry containing honey and pollen is fed to royal jelly in the first 3 days, they develop into worker bee. The same female larva, if fed with a large amount of royal jelly uninterruptedly in the larval stage, will develop into queen bee. When the worker bee larva grows to the end of 6 days, the worker bee seals the honeycomb opening with a wax cover. The larvae in the closed nest spin to cocoon and then pupate. The larvae and pupae of the cap are collectively called the cap, and the comb with most of the cap is called the cap spleen (pupa spleen). The sealing cover of the worker bee pupae is slightly protruded, and the whole sealing cover spleen looks relatively flat. The sealing cover of the drone pupae is convex, and the honeycomb is larger, so that the drone pupae and the drone pupae are easy to distinguish. Pupating the worker bee larva at the end of 2 days after the cap is closed.
The artificial breeding of the bee larvae is a technical difficulty obstacle, the humidity and the temperature are difficult to control, and the technical problems and problems that the temperature control and the humidity control are not constant, and particularly the humidity control is difficult to stabilize exist.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a honeybee larva breeding device, simple structure, convenient operation, it is with low costs, easily accuse control by temperature change is wet and stable, and the larva survival rate is high.
Solve above technical problem the utility model provides a honeybee larva breeding device, its characterized in that: the device is provided with an incubator and a climate box, wherein the incubator is positioned in the climate box; the incubator is provided with an incubator bottom surface, an incubator side surface, a cover, N baffles, a cell culture plate and a humidity/temperature device, wherein the cover is positioned at the top end of the incubator side surface, the incubator is hollow, the baffles are arranged on the incubator bottom surface, the humidity/temperature device is arranged on the incubator side surface, and the cell culture plate is positioned at the top end of the baffles; the climate box is formed by connecting an upper part, a middle part and a lower part in sequence, wherein the upper part is provided with a display, the lower part is provided with M heat dissipation holes, and the middle part is hollow; one end of the humidified gas input pipe is connected with the humidifier, and the other end of the humidified gas input pipe penetrates through the standby box and extends into the incubator from an opening at the top end of the incubator; the device is also provided with an incubator circuit wire, one end of the circuit wire is connected with the humidity/temperature probe, and the other end of the circuit wire is connected with the power supply base; the opening side of the interior of the climate box is provided with a climate box door for closing the climate box, so that a relatively closed space is formed in the interior of the climate box, the temperature and the stability of the temperature are kept, and the regulation and the operation are convenient.
The circuit seat can be a power socket externally arranged outside the climate box, and can also be a storage battery or a circuit board arranged in the climate box. When the weather box is a power socket, the circuit wire penetrates through a gap between the weather box door and the weather box, and a plug and the power socket which are arranged at the end port of the circuit wire are electrified.
The storage battery or the circuit board is provided with the switch, and the storage battery or the circuit board is placed in the climate box, so that the use is convenient and safer.
The humidity/temperature device is provided with a humidity/temperature probe and a humidity/temperature display, the humidity/temperature display and the probe are connected into a whole through an electric wire, the humidity/temperature display is arranged on the outer surface of the side surface of the incubator, and the humidity/temperature probe is arranged on the outer surface of the incubator or on the inner side surface of the incubator. When the humidity/temperature probe is arranged on the inner surface of the side surface of the incubator, a small hole is formed on the side surface, and an electric wire passes through the small hole to connect the humidity/temperature display and the humidity/temperature probe together.
The baffles are arranged on the bottom surface of the box in a staggered mode, and the saturated solution can be guaranteed to be communicated with the bottom of the whole box.
The height of the baffle is 4-6cm, and the optimal scheme is 5 cm.
N is more than or equal to 2; the M is more than 1, and is generally a plurality.
A liquid layer is further arranged in the box body and is positioned on the bottom surface in the box body, and bee larvae are cultured and humidity and temperature can be controlled. The liquid layer is a saturated solution prepared. The liquid layer is penetrated through the baffle layer arranged on the bottom surface. The liquid layer is at a height lower than the height of the baffle.
The side top or the upper portion of the larva incubator is provided with a jack, so that the humidifying gas input pipe can be directly penetrated into or taken out of the jack, and the operation is facilitated.
The cell culture plate is a 6-well, 24-well or 96-well cell culture plate.
The utility model discloses well incubator provides humidity for the larva growth in-process, and the larva is cultivated in 24 holes or 96 hole cell culture boards. The larva food is artificially prepared food, injected into the culture hole, and supplied to the larva. The incubator is placed in a constant temperature climatic chamber, and the set temperature is the natural suitable temperature of the bee larva.
The utility model discloses well display is the running state and the settlement control panel of the temperature, humidity, the illumination of weather case. The liquid input pipe is used for inputting mist humidifying gas to promote the environment humidity to reach more than 90 percent as far as possible, and the high humidity required by the growth of the bee larvae in the nearly closed larva incubator can be 94 percent finally through the humidifying action of the input humidifying gas and the chemical saturated solution at the bottom of the incubator.
The utility model discloses a natural environment of dark simulation honeybee, wherein humidity can reach more than 94%. Humidity varies with age, and larvae are stabilized at 94% and pupal stage at 75%. The climatic box has a light irradiation function and can be specially set to be a non-light environment according to the growth environment and the habit of the bee larvae. The temperature of the larvae is controlled at 35 ℃ in the whole process of cultivation.
The utility model is suitable for a cultivate bee larva, ability constant temperature and humidity (> 94%), can cultivate bee larva 2 days old and play to the pupa stage, the survival rate > 95%. The operation is simple, the cost is low, and the temperature is easy to control and is wet and stable.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments:
fig. 1 and 2 are schematic diagrams of the middle device of the present invention
FIG. 3-FIG. 6 are schematic structural views of the incubator of the present invention
FIG. 7 is a schematic diagram of the structure of the well cell culture plate of the present invention
Wherein the labels in the figure are:
1. the culture box body (1-1, bottom surface of the box, 1-2, side surface of the box, 1-3, cover), 2, baffle, 3, humidity/temperature device, 4, circuit line of the culture box, 5, humidified gas input tube, 6, climate box, 7, bracket, 8, standby box, 9, climate box door, 10, display, 11, heat dissipation hole, 12, cell culture plate, 13, liquid layer, 14, jack, 15, storage battery or circuit board
Detailed Description
The present invention will be further described with reference to the following detailed description:
example 1
A bee larva breeding device is provided with an incubator and a climatic chamber, wherein the incubator is positioned in the climatic chamber; the incubator is provided with an incubator bottom surface, an incubator side surface, a cover, N baffles, a cell culture plate and a humidity/temperature device, wherein the cover is positioned at the top end of the incubator side surface, the incubator is hollow, the baffles are arranged on the incubator bottom surface, the humidity/temperature device is arranged on the surface of the incubator side surface, and the cell culture plate is positioned at the top end of the baffles; the climate box is formed by connecting an upper part, a middle part and a lower part in sequence, wherein the upper part is provided with a display, the lower part is provided with M heat dissipation holes, and the middle part is hollow; one end of the humidified gas input pipe is connected with the humidifier, and the other end of the humidified gas input pipe penetrates through the standby box and extends into the incubator from an opening at the top end of the incubator; the device is also provided with an incubator circuit wire, one end of the circuit wire is connected with the humidity/temperature probe, and the other end of the circuit wire is connected with the power supply base; the opening side of the interior of the climate box is provided with a climate box door for closing the climate box, so that a relatively closed space is formed in the interior of the climate box, the temperature and the stability of the temperature are kept, and the regulation and the operation are convenient.
The circuit base is a power socket externally arranged outside the climate box, the circuit line penetrates through a gap between the climate box door and the climate box, and a plug arranged at the port of the circuit line is electrified with the power socket. This is low cost.
The humidity/temperature indicator is provided with a humidity/temperature probe and a humidity/temperature display, the humidity/temperature display and the probe are connected into a whole through an electric wire, the humidity/temperature display is arranged on the outer surface of the side surface of the incubator, and the humidity/temperature probe is also arranged on the outer surface of the incubator.
The cell culture plate is a 6-well cell culture plate. The baffles are arranged on the bottom surface of the box in a staggered manner, so that the saturated solution can be ensured to be communicated with the bottom of the whole box.
The height of the baffle is 4 or 6cm, N is 12, and M is 20.
A liquid layer is also arranged in the box body and is positioned on the bottom surface in the box body, and the liquid layer is used for culturing bee larvae and controlling humidity and temperature. The liquid layer is a saturated solution of some formulation. The liquid layer is penetrated through the baffle layer arranged on the bottom surface. The liquid layer is at a height lower than the height of the baffle.
The humidified gas supply pipe can extend into the incubator from the top of the side of the incubator, i.e. between the side of the incubator and the lid, for humidification.
Example 2
A bee larva breeding device is provided with an incubator and a climatic chamber, wherein the incubator is positioned in the climatic chamber; the incubator is provided with an incubator bottom surface, an incubator side surface, a cover, N baffles, a cell culture plate and a humidity/temperature device, wherein the cover is positioned at the top end of the incubator side surface, the incubator is hollow, the baffles are arranged on the incubator bottom surface, the humidity/temperature device is arranged on the surface of the incubator side surface, and the cell culture plate is positioned at the top end of the baffles; the climate box is formed by connecting an upper part, a middle part and a lower part in sequence, wherein the upper part is provided with a display, the lower part is provided with M heat dissipation holes, and the middle part is hollow; a door is fixedly arranged on one side surface of the middle part, a warming gas input pipe, a support, a standby box and a warmer are arranged in the door, the support is fixedly arranged on the inner side surface of the middle part, the incubator is arranged on the support, the standby box is arranged below the support, the humidifier is arranged in the standby box, one end of the humidifying gas input pipe is connected with the humidifier, and the other end of the humidifying gas input pipe penetrates through the standby box and extends into the incubator from an opening at the top end of the incubator; the device is also provided with an incubator circuit wire, one end of the circuit wire is connected with the humidity/temperature probe, and the other end of the circuit wire is connected with the power supply base; the opening side of the interior of the climate box is provided with a climate box door for closing the climate box, so that a relatively closed space is formed in the interior of the climate box, the temperature and the stability of the temperature are kept, and the regulation and the operation are convenient.
The circuit base is a storage battery or a circuit board placed in the climate box. The storage battery or the circuit board is provided with the switch, and the storage battery or the circuit board is placed in the climate box, so that the use is convenient and safer.
The humidity/temperature device is provided with a humidity/temperature probe and a humidity/temperature display, the humidity/temperature display and the probe are connected into a whole through an electric wire, the humidity/temperature display is arranged on the outer surface of the side surface of the incubator, and the humidity/temperature probe is arranged on the inner side surface of the incubator. The side surface of the incubator is provided with a small hole, and an electric wire passes through the small hole to connect the humidity/temperature display and the humidity/temperature probe together.
The cell culture plate is a 24-well or 96-well cell culture plate. The baffles are arranged on the bottom surface of the box in a staggered manner, so that the saturated solution can be ensured to be communicated with the bottom of the whole box.
The baffle is 5cm, and N is 10; m >1, may be plural.
A liquid layer is also arranged in the box body and is positioned on the bottom surface in the box body, and the liquid layer is used for culturing bee larvae and controlling humidity and temperature. The liquid layer is a saturated solution of some formulation. The liquid layer is penetrated through the baffle layer arranged on the bottom surface. The liquid layer is at a height lower than the height of the baffle.
The top end or the upper part of the side surface of the larva incubator is provided with a jack, so that the humidifying gas input pipe can be conveniently and directly penetrated into or taken out of the jack, and the operation is convenient.
The utility model discloses well transparent material for the incubator is pleasing to the eye also be convenient for observe the larva condition temporarily. The cover at the top of the box can be opened and closed at any time, and the vaseline is pasty and has a sealing effect to keep humidity. The cell culture plate is placed on a baffle plate, and a humidity and temperature probe is stuck on the wall. The top of the incubator is provided with a cover, and the seal is sealed by Vaseline to achieve a closed state.
The baffle and the cell culture plate can be overlapped for a plurality of layers, and the cell culture plate is directly and flatly placed on the bottom baffle. One layer of baffle, one layer of cell culture plate, the other layer of baffle and one layer of cell culture plate. The cell culture plates were loaded with the corresponding solutions and self-prepared food for feeding larvae.
The nutrient substances in the cell culture plate are added by opening the door of the climatic chamber and then opening the cover of the incubator each time, and then the incubator is put into the climatic chamber. The feed is fed once in the morning and at night every day, the feed amount is different according to the age of the larvae, 50 microliter is generally fed every time when the larvae are aged for 1 to 2 days, and more than 100 microliter is fed every time when the larvae are aged for 3 days.
The utility model is suitable for breeding bee larva; a chemical solution moisturizing device is adopted, and saturated chemical solution is added to the bottom in an incubator, so that a larva culture environment with 94% of humidity and 35 ℃ is guaranteed to be realized in the approximately closed incubator.
The utility model applies and obtains special fund funding for the construction of the modern agricultural industry technology system (No. CARS-44-KXJ 21).
The utility model discloses the operation explanation: putting the incubator into a climatic incubator, adjusting the temperature to 35 ℃, uncovering the incubator, pouring a prepared chemical saturated solution (the moisturizing solution can be repeatedly used for 1 to 2 times), and switching on a humidifier steam conduit to adjust the flow to a proper value. Larvae of the appropriate age (2, 3 days old) were picked from the spleens of honey comb with a larva-transfering needle and placed flat in 24-well cell plates. The plates were then placed in a larval incubator. The food is administered 1-2 times daily. Can breed worker bee or drone larva to eclosion stage.
The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above, and the above embodiments and description have been described only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention, which will also have various changes and modifications, all of which will fall within the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A bee larva breeding device which is characterized in that: the device is provided with an incubator and a climate box, wherein the incubator is positioned in the climate box; the incubator is provided with an incubator bottom surface, an incubator side surface, a cover, N baffles, a cell culture plate and a humidity/temperature device, wherein the cover is positioned at the top end of the incubator side surface, the incubator is hollow, the baffles are arranged on the incubator bottom surface, the humidity/temperature device is arranged on the outer surface of the incubator side surface, and the cell culture plate is positioned at the top end of the baffles; the climate box is formed by connecting an upper part, a middle part and a lower part in sequence, wherein the upper part is provided with a display, the lower part is provided with M heat dissipation holes, and the middle part is hollow; one end of the humidified gas input pipe is connected with the humidifier, and the other end of the humidified gas input pipe penetrates through the standby box and extends into the incubator from an opening at the top end of the incubator; the device is also provided with an incubator circuit wire, one end of the circuit wire is connected with the humidity/temperature probe, and the other end of the circuit wire is connected with the power supply base; and a climate box door is arranged on the opening side of the middle part of the climate box and used for closing the climate box.
2. A bee larva cultivating device according to claim 1, wherein: the power socket is a power socket externally arranged outside the climate box, or a storage battery or a circuit board arranged in the climate box.
3. A bee larva cultivating device according to claim 1, wherein: the humidity/temperature device is provided with a humidity/temperature probe and a humidity/temperature display, the humidity/temperature display and the probe are connected into a whole through an electric wire, the humidity/temperature display is arranged on the outer surface of the side surface of the incubator, and the humidity/temperature probe is arranged on the outer surface of the incubator or on the inner side surface of the incubator.
4. A bee larva cultivating device according to claim 3, wherein: when the humidity/temperature probe is arranged on the inner surface of the side surface of the incubator, a small hole is formed in the side surface, and an electric wire penetrates through the small hole to connect the humidity/temperature display and the humidity/temperature probe together.
5. A bee larva cultivating device according to claim 1, wherein: the baffles are arranged on the bottom surface of the box in a staggered manner.
6. A bee larva cultivating device according to claim 5, wherein: the height of the baffle is 4-6cm, and the optimal scheme is 5 cm.
7. A bee larva cultivating device according to claim 1, wherein: n is more than or equal to 2; the M > 1.
8. A bee larva cultivating device according to claim 1, wherein: still be equipped with the one deck liquid layer in the incubator box, be located on the incasement bottom surface.
9. A bee larva cultivating device according to claim 1, wherein: the cell culture plate is a 6-well, 24-well or 96-well cell culture plate.
10. A bee larva cultivating device according to claim 1, wherein: the top end or the upper part of the side surface of the larva incubator is provided with a jack for passing through the humidified gas input pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920962766.6U CN210328951U (en) | 2019-06-25 | 2019-06-25 | Bee larva breeding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920962766.6U CN210328951U (en) | 2019-06-25 | 2019-06-25 | Bee larva breeding device |
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| Publication Number | Publication Date |
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| CN210328951U true CN210328951U (en) | 2020-04-17 |
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| CN201920962766.6U Active CN210328951U (en) | 2019-06-25 | 2019-06-25 | Bee larva breeding device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113491258A (en) * | 2021-06-22 | 2021-10-12 | 重庆师范大学 | Butterfly wedding flying room constructed manually |
-
2019
- 2019-06-25 CN CN201920962766.6U patent/CN210328951U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113491258A (en) * | 2021-06-22 | 2021-10-12 | 重庆师范大学 | Butterfly wedding flying room constructed manually |
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